##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295141_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 672046 Sequences flagged as poor quality 0 Sequence length 100 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.238317019965894 33.0 33.0 33.0 27.0 33.0 2 29.830321138731573 33.0 33.0 33.0 22.0 33.0 3 30.696275254967667 33.0 33.0 33.0 27.0 33.0 4 34.97820536094255 37.0 37.0 37.0 33.0 37.0 5 35.38091291369936 37.0 37.0 37.0 33.0 37.0 6 35.15616788136526 37.0 37.0 37.0 33.0 37.0 7 35.03255729518515 37.0 37.0 37.0 33.0 37.0 8 35.02675412099767 37.0 37.0 37.0 33.0 37.0 9 34.955907780122196 37.0 37.0 37.0 33.0 37.0 10-11 34.92853762986462 37.0 37.0 37.0 33.0 37.0 12-13 34.84469366680257 37.0 37.0 37.0 30.0 37.0 14-15 36.18483332986135 37.0 37.0 40.0 27.0 40.0 16-17 36.165258776928965 37.0 37.0 40.0 27.0 40.0 18-19 36.212919204935375 37.0 37.0 40.0 27.0 40.0 20-21 36.213575410016574 37.0 37.0 40.0 27.0 40.0 22-23 36.1926557110674 37.0 37.0 40.0 27.0 40.0 24-25 36.15476619159998 37.0 37.0 40.0 27.0 40.0 26-27 36.07456260434554 37.0 37.0 40.0 27.0 40.0 28-29 35.98082571728721 37.0 37.0 40.0 27.0 40.0 30-31 35.796017683313345 37.0 37.0 40.0 27.0 40.0 32-33 35.693493451341126 37.0 37.0 40.0 27.0 40.0 34-35 35.57560271171914 37.0 37.0 40.0 27.0 40.0 36-37 35.52624373926785 37.0 37.0 40.0 27.0 40.0 38-39 35.40602205801389 37.0 35.0 40.0 27.0 40.0 40-41 35.257183288048736 37.0 33.0 40.0 27.0 40.0 42-43 35.05343681831303 37.0 33.0 40.0 27.0 40.0 44-45 34.83385214702565 37.0 33.0 40.0 22.0 40.0 46-47 34.57012168809874 37.0 33.0 40.0 22.0 40.0 48-49 34.32458046026612 37.0 33.0 40.0 22.0 40.0 50-51 34.17170699624728 37.0 33.0 40.0 22.0 40.0 52-53 34.058561467518594 37.0 33.0 40.0 22.0 40.0 54-55 33.91385411117692 37.0 33.0 40.0 22.0 40.0 56-57 33.713599217910684 37.0 33.0 40.0 22.0 40.0 58-59 33.55478494031659 37.0 33.0 40.0 22.0 40.0 60-61 33.332687792204695 37.0 33.0 37.0 22.0 40.0 62-63 33.112184285004304 37.0 33.0 37.0 22.0 40.0 64-65 32.92695961288364 37.0 33.0 37.0 22.0 40.0 66-67 32.65909253235642 37.0 33.0 37.0 22.0 40.0 68-69 32.42513384500466 37.0 33.0 37.0 22.0 40.0 70-71 32.162030575288 37.0 33.0 37.0 22.0 37.0 72-73 31.932953250223946 37.0 33.0 37.0 22.0 37.0 74-75 31.71423459108454 33.0 33.0 37.0 18.5 37.0 76-77 31.50132282611607 33.0 27.0 37.0 15.0 37.0 78-79 31.307037762296034 33.0 27.0 37.0 15.0 37.0 80-81 31.177824137038236 33.0 27.0 37.0 15.0 37.0 82-83 31.04506387955586 33.0 27.0 37.0 15.0 37.0 84-85 30.887304589269185 33.0 27.0 37.0 15.0 37.0 86-87 30.94898712290528 33.0 27.0 37.0 15.0 37.0 88-89 31.007292655562267 33.0 27.0 37.0 15.0 37.0 90-91 31.069330819616514 33.0 27.0 37.0 15.0 37.0 92-93 31.081220630730634 33.0 27.0 37.0 15.0 37.0 94-95 31.163611568255746 33.0 27.0 37.0 15.0 37.0 96-97 31.153259598301304 33.0 27.0 37.0 15.0 37.0 98-99 31.08367507581326 33.0 27.0 37.0 15.0 37.0 100 30.492394865827638 33.0 27.0 37.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5352133187593857 1101 2 4.100978582019362 1101 3 2.2142771777972 1101 4 1.1645889925586204 1101 5 0.3454009169646426 1101 6 0.20208075854581864 1101 7 0.2903769342849998 1101 8 0.32827697513306475 1101 9 0.5088445529230086 1101 10-11 0.3875474892812818 1101 12-13 0.3605883300213861 1101 14-15 0.7721888769098229 1101 16-17 0.7643868539722121 1101 18-19 0.7959341055513605 1101 20-21 0.6992547997573553 1101 22-23 0.7145170127600693 1101 24-25 0.7580985121552217 1101 26-27 0.8188678873812378 1101 28-29 0.6208330481382021 1101 30-31 0.397830972563213 1101 32-33 0.7728252933654076 1101 34-35 0.8860849628979892 1101 36-37 0.7897613736766544 1101 38-39 0.8273071904647367 1101 40-41 0.6832111886899028 1101 42-43 0.9456807229145738 1101 44-45 0.8654999518484345 1101 46-47 0.8903446708695597 1101 48-49 0.7796150599803937 1101 50-51 0.627344821120495 1101 52-53 0.5996994471354924 1101 54-55 0.5542649177807917 1101 56-57 0.664712084562467 1101 58-59 0.5749688157682584 1101 60-61 0.6962043992951052 1101 62-63 0.6899113037455677 1101 64-65 0.6280631261524121 1101 66-67 0.714727721553011 1101 68-69 0.605647416572225 1101 70-71 0.749222790372631 1101 72-73 0.7069848499557274 1101 74-75 0.6561936633179499 1101 76-77 0.7289824683109991 1101 78-79 0.7846724621634671 1101 80-81 0.9025763112422673 1101 82-83 0.9774204402690998 1101 84-85 0.9789815472672068 1101 86-87 0.853859507788119 1101 88-89 0.6648279291173829 1101 90-91 0.77837198554165 1101 92-93 0.6540343406987503 1101 94-95 0.66128141898416 1101 96-97 0.575889270594331 1101 98-99 0.603051952824071 1101 100 0.6437593797857843 1102 1 0.6352069010979804 1102 2 4.134735481005372 1102 3 2.2373101645768187 1102 4 1.0913121346456407 1102 5 0.5996943324440451 1102 6 0.6464075458645198 1102 7 0.5408390059061006 1102 8 0.426056464287214 1102 9 0.5191834054451547 1102 10-11 0.5297564483365989 1102 12-13 0.6507885610571975 1102 14-15 0.7539177948921107 1102 16-17 0.7591083274672741 1102 18-19 0.8585961515018141 1102 20-21 0.6940308233743551 1102 22-23 0.870081125197494 1102 24-25 0.7686523563344068 1102 26-27 0.7589012592205364 1102 28-29 0.7914369500763385 1102 30-31 0.684174189095117 1102 32-33 0.8604039097176042 1102 34-35 1.0308641953456927 1102 36-37 0.6811007396117077 1102 38-39 0.6995957285214658 1102 40-41 0.7936398884717022 1102 42-43 0.8944356966021587 1102 44-45 0.7489359677642398 1102 46-47 0.8839976037408235 1102 48-49 0.6507355836615645 1102 50-51 0.6902764088499254 1102 52-53 0.683902373589099 1102 54-55 0.5984761871942155 1102 56-57 0.5146639521019409 1102 58-59 0.6099482792517605 1102 60-61 0.6388722211408293 1102 62-63 0.7591931674344394 1102 64-65 0.6090957278723437 1102 66-67 0.791672272958472 1102 68-69 0.5027434247868356 1102 70-71 0.5490976459752481 1102 72-73 0.6819110468495744 1102 74-75 0.6094089116814487 1102 76-77 0.6627714556040303 1102 78-79 0.7226971059832614 1102 80-81 0.6174139444087423 1102 82-83 0.6593290527707083 1102 84-85 0.5462386387830556 1102 86-87 0.5139737421611095 1102 88-89 0.3669361308886572 1102 90-91 0.5147344550577593 1102 92-93 0.7633303232427124 1102 94-95 0.6532112097683971 1102 96-97 0.5213808634578925 1102 98-99 0.5880249986461727 1102 100 0.7109394593647842 1103 1 0.4357504678069617 1103 2 3.940646362104225 1103 3 2.1088689316243077 1103 4 0.9078730173719407 1103 5 0.4166360373556728 1103 6 0.45515018167482424 1103 7 0.4368354906243894 1103 8 0.3420184471664882 1103 9 0.3901139786313621 1103 10-11 0.357862427570403 1103 12-13 0.386829963264006 1103 14-15 0.5737693718865415 1103 16-17 0.4893430050703742 1103 18-19 0.6275607708423792 1103 20-21 0.6355030103352632 1103 22-23 0.5774827461329508 1103 24-25 0.48179723150935416 1103 26-27 0.5083918688615299 1103 28-29 0.4832264888583566 1103 30-31 0.6903031478556514 1103 32-33 0.8209188333904223 1103 34-35 0.8262682899979055 1103 36-37 0.6118073763333811 1103 38-39 0.4747072085201651 1103 40-41 0.49506715794407796 1103 42-43 0.6838931063603226 1103 44-45 0.6826403586114864 1103 46-47 0.5776342928662288 1103 48-49 0.5476060064718311 1103 50-51 0.31088149298884815 1103 52-53 0.3819396746339123 1103 54-55 0.5961212739370509 1103 56-57 0.5344276470536045 1103 58-59 0.5728360180793501 1103 60-61 0.3061377876545137 1103 62-63 0.4564981181228447 1103 64-65 0.5654704308635843 1103 66-67 0.41921352869522366 1103 68-69 0.5050641616940368 1103 70-71 0.7457874223642698 1103 72-73 0.4919898151990232 1103 74-75 0.45786121126156587 1103 76-77 0.34843203562551395 1103 78-79 0.4322078959451794 1103 80-81 0.5532714453038388 1103 82-83 0.6328261070948287 1103 84-85 0.6242665006455077 1103 86-87 0.5199920346455684 1103 88-89 0.49575655858126666 1103 90-91 0.4989319618116532 1103 92-93 0.47272719203807867 1103 94-95 0.5698290530779708 1103 96-97 0.5487593746709933 1103 98-99 0.37380763706039133 1103 100 0.5966862939838009 1104 1 0.4724562024980372 1104 2 3.656374227476668 1104 3 2.188895641314751 1104 4 1.0096137870070763 1104 5 0.4019150947867729 1104 6 0.3990627016987247 1104 7 0.43772565419732246 1104 8 0.2945106899242873 1104 9 0.2985524518740803 1104 10-11 0.5100404583716909 1104 12-13 0.504629551617171 1104 14-15 0.8682991947578671 1104 16-17 0.6659029557454588 1104 18-19 0.7362633275394757 1104 20-21 0.6584241244316544 1104 22-23 0.6515538262443243 1104 24-25 0.6201538106600069 1104 26-27 0.7171832984182984 1104 28-29 0.8225388099945476 1104 30-31 0.7289740824604394 1104 32-33 0.9570815730852615 1104 34-35 0.9390641163217808 1104 36-37 0.771581214516857 1104 38-39 0.9102837126024355 1104 40-41 0.795960382799592 1104 42-43 0.8106132242962332 1104 44-45 1.008747072051996 1104 46-47 1.015249180648702 1104 48-49 0.8743163665695519 1104 50-51 0.8043676576657575 1104 52-53 0.6974354565213545 1104 54-55 0.8161354132359335 1104 56-57 0.8134228958473813 1104 58-59 0.8995009372638805 1104 60-61 1.0821982709449358 1104 62-63 1.0481007808646936 1104 64-65 0.9262729556563869 1104 66-67 0.7961016584285474 1104 68-69 0.6970556073225538 1104 70-71 0.7853310616648308 1104 72-73 0.9677707651245662 1104 74-75 0.9089904657866441 1104 76-77 0.9526823394817221 1104 78-79 0.9254948380921988 1104 80-81 0.7348827513856939 1104 82-83 0.6764013120453036 1104 84-85 0.7941487895473536 1104 86-87 0.791889891125944 1104 88-89 0.9919521974972483 1104 90-91 1.0598328303070872 1104 92-93 1.106602579661022 1104 94-95 0.9138818181097044 1104 96-97 1.0192060397145397 1104 98-99 0.7063994862998761 1104 100 0.8642686986882282 1105 1 0.48247485557243763 1105 2 3.655299841087995 1105 3 2.082409081456799 1105 4 0.9348364943552525 1105 5 0.5525037769895107 1105 6 0.398693381377619 1105 7 0.3972251244384992 1105 8 0.2987262175894969 1105 9 0.30219385431283996 1105 10-11 0.3674348507462355 1105 12-13 0.317107398393361 1105 14-15 0.42099616839367116 1105 16-17 0.5138000602261741 1105 18-19 0.6612934607202305 1105 20-21 0.506486703601702 1105 22-23 0.4498044470142304 1105 24-25 0.5158021141127733 1105 26-27 0.4364741938090404 1105 28-29 0.400021014270898 1105 30-31 0.4618235901852259 1105 32-33 0.3797485358477033 1105 34-35 0.5631698934988307 1105 36-37 0.3184611532768358 1105 38-39 0.5096778833847821 1105 40-41 0.5146395014766014 1105 42-43 0.5750621624043859 1105 44-45 0.6548015079487826 1105 46-47 0.7524197361304417 1105 48-49 0.41293432196428626 1105 50-51 0.39862827598471995 1105 52-53 0.47064615952019295 1105 54-55 0.6849936043049993 1105 56-57 0.3512993327792202 1105 58-59 0.40743700743665556 1105 60-61 0.3905421283086312 1105 62-63 0.5457131489705702 1105 64-65 0.438375870361142 1105 66-67 0.6114139187600429 1105 68-69 0.6810796256139753 1105 70-71 0.5560327846640369 1105 72-73 0.5685846607776703 1105 74-75 0.5780413269505047 1105 76-77 0.4663390375374199 1105 78-79 0.5202744234078303 1105 80-81 0.6517089372516658 1105 82-83 0.6422418203451805 1105 84-85 0.5375440711684867 1105 86-87 0.47219878680542493 1105 88-89 0.5221899390603149 1105 90-91 0.5101021703412663 1105 92-93 0.5594552433958313 1105 94-95 0.5728852479923283 1105 96-97 0.5459184509397659 1105 98-99 0.4494858818342671 1105 100 0.6758198872041881 1106 1 0.4772766056921469 1106 2 3.7207899027740794 1106 3 2.1539348966614327 1106 4 0.917465335652075 1106 5 0.395864307283297 1106 6 0.21763110378083894 1106 7 0.26279874870732556 1106 8 0.10957151414277178 1106 9 0.158294392305649 1106 10-11 0.16529367692666597 1106 12-13 0.1208752258601038 1106 14-15 0.13272685276183438 1106 16-17 0.23130336986780264 1106 18-19 0.4485621378963742 1106 20-21 0.40310500099060675 1106 22-23 0.3204026236598949 1106 24-25 0.35454369917291473 1106 26-27 0.3499528853504401 1106 28-29 0.31495759120949884 1106 30-31 0.2806190684166978 1106 32-33 0.29874376620814047 1106 34-35 0.30510704316974824 1106 36-37 0.2475134690870604 1106 38-39 0.17155081192468913 1106 40-41 0.12454800514302633 1106 42-43 0.4933360786727974 1106 44-45 0.46802552139390485 1106 46-47 0.16950687594285085 1106 48-49 -0.07644501460514164 1106 50-51 0.006612258258179793 1106 52-53 0.12145479181666019 1106 54-55 -0.08923930996354557 1106 56-57 -0.05445122821676307 1106 58-59 0.04698816369869263 1106 60-61 0.17958604091531782 1106 62-63 0.21086356161950448 1106 64-65 0.07458123714454246 1106 66-67 0.06309385529004174 1106 68-69 0.13013602566280014 1106 70-71 0.09509289338928184 1106 72-73 0.21977331504452735 1106 74-75 0.04168456166746637 1106 76-77 0.09699007455846242 1106 78-79 0.2595995381890184 1106 80-81 0.19819625733390822 1106 82-83 0.3754994935497571 1106 84-85 0.13058772088165327 1106 86-87 0.2566887733790786 1106 88-89 0.32027691925880575 1106 90-91 0.26946141433462145 1106 92-93 0.45956087025603765 1106 94-95 0.21124634162936573 1106 96-97 0.4243698672785996 1106 98-99 0.34345552702190574 1106 100 0.2792835093135331 1107 1 0.3289631779819935 1107 2 3.458115808066559 1107 3 2.154249698050375 1107 4 1.0743375453670367 1107 5 0.3846440226265244 1107 6 0.21141711515184625 1107 7 0.07622073519948458 1107 8 0.18230208231158684 1107 9 0.3350238920576274 1107 10-11 0.11910658950727537 1107 12-13 0.131157783955274 1107 14-15 0.15329673867017846 1107 16-17 0.3154984322557084 1107 18-19 0.23338107940055153 1107 20-21 0.2676688579753801 1107 22-23 0.21046825497977295 1107 24-25 0.18192855818004716 1107 26-27 0.1783861283771202 1107 28-29 0.22622557304641333 1107 30-31 0.12292932903409337 1107 32-33 0.16118319078936594 1107 34-35 0.5215616163753864 1107 36-37 0.3302461359317945 1107 38-39 0.3535546658568478 1107 40-41 0.44971780710433507 1107 42-43 0.37997991428923683 1107 44-45 0.5666661517598399 1107 46-47 0.4277079290965915 1107 48-49 0.43337869661704786 1107 50-51 0.39994991613584574 1107 52-53 0.20974513409162654 1107 54-55 0.5065404435374248 1107 56-57 0.2984468524487056 1107 58-59 0.395509551114273 1107 60-61 0.2741809964615456 1107 62-63 0.475552385216254 1107 64-65 0.3236292014288935 1107 66-67 0.3277006397369391 1107 68-69 0.24040141277311022 1107 70-71 0.40760767951512733 1107 72-73 0.3203847928939574 1107 74-75 0.2990442365634891 1107 76-77 0.283705455945114 1107 78-79 0.3259587170343643 1107 80-81 0.23768093699964865 1107 82-83 0.4863973452687631 1107 84-85 0.6392810089344607 1107 86-87 0.3759775130287899 1107 88-89 0.27688297870856715 1107 90-91 0.39395773211231244 1107 92-93 0.5131133562330668 1107 94-95 0.2938702589033717 1107 96-97 0.31130942541192397 1107 98-99 0.24962468030156515 1107 100 0.46599507492577175 1108 1 0.41963680298271555 1108 2 3.6467401446538297 1108 3 2.0339417128422888 1108 4 0.922633799455312 1108 5 0.22653522775171808 1108 6 0.14451999056596776 1108 7 0.08219409666389055 1108 8 -0.07031980564024565 1108 9 0.05763170805575868 1108 10-11 0.0695864508282682 1108 12-13 0.02787979838142718 1108 14-15 -0.0385902795194113 1108 16-17 -0.05887304614644506 1108 18-19 0.02898715517349615 1108 20-21 -0.1522918522058987 1108 22-23 -0.05391362819798928 1108 24-25 -0.12362606159807399 1108 26-27 -0.20322634233839665 1108 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0.30775252350502313 2211 98-99 0.2840907444059191 2211 100 0.22230425007911236 2212 1 0.6718761841958347 2212 2 4.280405287389467 2212 3 2.515868510992309 2212 4 1.3017909415530227 2212 5 0.960801082836511 2212 6 0.8016142439283627 2212 7 0.7251011723111205 2212 8 0.6001668776833426 2212 9 0.4455177853509582 2212 10-11 0.6307441511100222 2212 12-13 0.40371242290962783 2212 14-15 0.5612568843740604 2212 16-17 0.6494013677603192 2212 18-19 0.7860621378963728 2212 20-21 0.8365085837092607 2212 22-23 0.7375654266103666 2212 24-25 0.7929393788357118 2212 26-27 0.6134407673314755 2212 28-29 0.40261565232646745 2212 30-31 0.3926448850658062 2212 32-33 0.7858617851754772 2212 34-35 0.43948533610969065 2212 36-37 0.7809960823641902 2212 38-39 0.9354997054968734 2212 40-41 0.8195163929196312 2212 42-43 0.6270952989046208 2212 44-45 0.9118611378322683 2212 46-47 0.9332977083980722 2212 48-49 0.4267952382926481 2212 50-51 0.4138962413983265 2212 52-53 0.3416339277492213 2212 54-55 0.5059003106897748 2212 56-57 0.620864894017167 2212 58-59 0.5271251500000602 2212 60-61 0.44465453406599664 2212 62-63 0.24993100102940957 2212 64-65 0.45686522028469057 2212 66-67 0.7862366371657004 2212 68-69 0.7688083122802922 2212 70-71 0.5559069081416546 2212 72-73 0.7732506596177622 2212 74-75 0.3760101675684169 2212 76-77 0.40243001133401535 2212 78-79 0.46667277317321165 2212 80-81 0.3616577957954483 2212 82-83 0.2820985451830573 2212 84-85 0.7899264985423464 2212 86-87 0.6064253381841347 2212 88-89 0.9137964134632277 2212 90-91 0.5380599390975291 2212 92-93 0.6732858439125202 2212 94-95 0.8156588811446426 2212 96-97 0.5599863056347658 2212 98-99 0.6999755480967202 2212 100 0.7846312437708569 2213 1 -0.7198731954567705 2213 2 -25.72921009722592 2213 3 -14.635062010099503 2213 4 -6.545334782185606 2213 5 -2.5138818507173895 2213 6 -1.7712283256883978 2213 7 -1.3527386402061126 2213 8 -1.1915135744809788 2213 9 -1.0598378804885726 2213 10-11 -1.040936990329186 2213 12-13 -1.1926171883393337 2213 14-15 -1.349158059105406 2213 16-17 -1.2405076479551553 2213 18-19 -1.1513482563688626 2213 20-21 -1.4307991085984355 2213 22-23 -1.2940619513879739 2213 24-25 -1.1015912249690842 2213 26-27 -1.2538117829855864 2213 28-29 -1.355300669702757 2213 30-31 -1.3503624212479508 2213 32-33 -1.3925914819874592 2213 34-35 -1.0204195439564856 2213 36-37 -1.4560179788434127 2213 38-39 -1.5904342619693494 2213 40-41 -1.3800562566530203 2213 42-43 -1.4439448168935627 2213 44-45 -1.2890349195154087 2213 46-47 -1.5207862452826575 2213 48-49 -1.4348397217294107 2213 50-51 -1.0164421925691371 2213 52-53 -1.381904809499197 2213 54-55 -1.2891472492771427 2213 56-57 -1.096491047532325 2213 58-59 -1.1265486851253854 2213 60-61 -1.1537325627081927 2213 62-63 -0.9090571434426025 2213 64-65 -1.1982043964784737 2213 66-67 -1.3355203245106182 2213 68-69 -1.6920960163659728 2213 70-71 -1.3749898384809 2213 72-73 -1.5508188192904235 2213 74-75 -1.5252374199694927 2213 76-77 -1.5573404601300034 2213 78-79 -1.3894307559141872 2213 80-81 -0.8755492623793515 2213 82-83 -1.2522721468489273 2213 84-85 -1.226254229331932 2213 86-87 -0.9394459052677533 2213 88-89 -1.3362311575320867 2213 90-91 -1.2797026746328264 2213 92-93 -0.8454693256491019 2213 94-95 -1.2021761064484089 2213 96-97 -1.558382869992478 2213 98-99 -1.4578787391930526 2213 100 -1.8174710446217546 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 6.0 5 9.0 6 10.0 7 14.0 8 14.0 9 23.0 10 113.0 11 552.0 12 1606.0 13 2750.0 14 3589.0 15 4327.0 16 5040.0 17 5232.0 18 5414.0 19 5690.0 20 6477.0 21 7751.0 22 9225.0 23 9414.0 24 9188.0 25 8654.0 26 8817.0 27 9535.0 28 11141.0 29 13383.0 30 15664.0 31 19523.0 32 25371.0 33 33483.0 34 45424.0 35 64563.0 36 98811.0 37 152250.0 38 88774.0 39 208.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.126120445025 19.11520502079486 10.557632539074817 30.201041995105328 2 14.258927797008427 21.632038352664736 33.135472272379616 30.973561577947216 3 17.36488276100148 25.677706585561104 27.397826934465797 29.559583718971616 4 12.459971191162115 18.533189685959858 32.04105259398586 36.96578652889217 5 12.866934227919863 37.44551035098413 32.69677003989574 16.990785381200272 6 32.524292802898906 34.68254476322054 16.638802086047214 16.154360347833336 7 27.36908695781608 32.50781459319461 20.95605965883719 19.167038790152123 8 27.842597278871057 32.57316383339784 19.27223198070797 20.312006907023132 9 27.191748501371727 15.318533867435965 20.23070018354196 37.25901744765035 10-11 24.993710579475202 26.153048024377647 25.79369509782468 23.059546298322473 12-13 26.437252146574412 23.98447082921485 26.468364078074575 23.109912946136166 14-15 25.15470624050066 24.510351213310766 24.702236076438354 25.63270646975022 16-17 22.577620128205222 27.285251040653396 27.048206086957173 23.088922744184213 18-19 23.373064660651398 25.937119518461078 29.08531121715393 21.60450460373359 20-21 23.42181775696414 25.204777280754172 29.175153317849613 22.198251644432073 22-23 22.74613083964705 24.963851620109736 28.007682879546824 24.282334660696385 24-25 22.833259910582996 25.68149210903874 27.570292361454246 23.91495561892402 26-27 23.232187941576317 24.701709270061098 28.30448833140984 23.76161445695275 28-29 22.844003928922223 24.310280679822604 29.433505967794744 23.41220942346043 30-31 23.48983200180976 25.230089654597627 28.414286394646922 22.865791948945695 32-33 21.928638070112 25.101147403647705 28.766113276164035 24.20410125007626 34-35 21.889157314949085 25.443268809396084 28.893291836830354 23.774282038824477 36-37 24.14738781261951 24.77980664352262 27.846591560110294 23.226213983747577 38-39 23.99303878888617 24.61081279356914 27.856600241514446 23.53954817603025 40-41 22.717282286170065 25.0979901965161 28.020211129067302 24.164516388246536 42-43 23.856873195234265 24.756865829223123 28.07901125809238 23.30724971745023 44-45 24.214068821015804 24.488254652788473 27.16381729178804 24.13385923440768 46-47 23.517715824996 24.985508109133796 28.291519546379636 23.205256519490568 48-49 24.483760444604528 24.936000285766166 27.24385974834717 23.33637952128214 50-51 23.245544514668552 25.525285516444868 26.94752837676636 24.281641592120216 52-53 22.909412138563564 25.563053089935934 25.901521246170095 25.62601352533041 54-55 23.31382576546863 25.89317076365282 25.592371211354475 25.20063225952408 56-57 23.87989287630398 24.9523948426444 26.088147115748804 25.079565165302814 58-59 23.544609166125323 24.186406108269843 27.207502438828378 25.061482286776453 60-61 23.886342040899542 25.1416185551745 26.627413542551047 24.34462586137491 62-63 22.518623577884917 25.266824470685584 27.003313586747442 25.21123836468206 64-65 22.2322185337569 25.62119138670173 26.96868425967887 25.177905819862495 66-67 23.732825716979576 24.881139041090282 26.4875572724154 24.89847796951474 68-69 24.016667150398035 24.867364579403013 26.090014221702273 25.025954048496672 70-71 23.10203686752547 25.009637352741994 26.544022980807156 25.344302798925376 72-73 23.985798122589795 24.802755836953754 26.504168136416606 24.70727790403984 74-75 24.08267633912957 24.469654235249816 26.23501762981992 25.212651795800696 76-77 23.324559973985018 24.970736865447623 27.383745547278288 24.32095761328907 78-79 23.714969439644975 24.987461846062722 26.893484475756345 24.40408423853596 80-81 22.726904065473793 25.1527054668892 26.988073012894304 25.132317454742704 82-83 22.3815175133466 25.24660056920702 26.104875462713217 26.26700645473316 84-85 22.65031069257173 25.118514092592413 25.902481745741788 26.328693469094073 86-87 23.000999286436567 24.520539689082778 26.473054609552822 26.005406414927833 88-89 22.83697040810784 24.122105711158547 27.152352373504723 25.888571507228885 90-91 22.929907870241536 24.646161309668194 26.759223020683436 25.664707799406834 92-93 21.53772094033755 25.036159960953196 27.066846821643598 26.359272277065653 94-95 21.25378326820805 25.069564518673047 27.25247679446698 26.424175418651924 96-97 22.69075244497186 24.64970593377651 26.686035481386966 25.973506139864668 98-99 22.504867616456377 24.383763848619918 26.505799103778255 26.605569431145454 100 21.80510373097868 24.669770850462946 27.323818205650685 26.201307212907686 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 149.0 1 109.0 2 60.0 3 48.5 4 41.5 5 86.5 6 279.0 7 622.0 8 945.0 9 989.5 10 682.0 11 385.5 12 290.5 13 279.0 14 304.0 15 350.0 16 396.5 17 427.5 18 464.0 19 521.0 20 594.5 21 648.5 22 730.5 23 911.0 24 1093.0 25 1301.0 26 1653.0 27 2080.0 28 2628.0 29 3273.0 30 3792.0 31 4403.0 32 5414.0 33 6218.0 34 7119.5 35 8848.5 36 10767.0 37 12654.0 38 14845.0 39 16602.0 40 17883.0 41 18905.5 42 19348.5 43 20677.5 44 21826.5 45 21809.5 46 22942.5 47 25134.5 48 26735.5 49 30292.5 50 43439.5 51 44031.0 52 29331.0 53 23262.0 54 21299.0 55 19696.5 56 18394.5 57 16588.0 58 15524.5 59 15532.5 60 14837.0 61 13282.0 62 11854.0 63 9753.5 64 7540.0 65 6385.0 66 5278.0 67 4224.0 68 3368.5 69 2438.5 70 1728.5 71 1326.0 72 1138.5 73 927.5 74 762.5 75 643.5 76 395.0 77 275.5 78 196.5 79 67.5 80 20.0 81 9.0 82 4.0 83 3.0 84 2.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.8951768182535125 2 5.644107695008973 3 0.0 4 0.004463980144216319 5 0.15579290703314952 6 0.05133577165848766 7 0.033628650419762936 8 0.03958062394538469 9 0.03943182460724415 10-11 0.042110212693773935 12-13 0.04136621600307122 14-15 0.0430030087226172 16-17 0.034595846117676465 18-19 0.029759867628108793 20-21 0.023435895757135672 22-23 0.024179892447838393 24-25 0.020534308663395062 26-27 0.013763938778000315 28-29 0.015772729842897657 30-31 0.01993911131083289 32-33 0.0010415953669838077 34-35 0.0031991857700216946 36-37 0.0034223847772325107 38-39 0.0054311758421298545 40-41 0.005951973525621758 42-43 0.00602637319469203 44-45 0.008407162604940734 46-47 0.017632721569654456 48-49 0.024998288807611384 50-51 0.022245501052011323 52-53 0.02909027060647634 54-55 0.025295887483892474 56-57 0.016739925540811194 58-59 0.015549530835686843 60-61 0.012275945396594877 62-63 0.017186323555232825 64-65 0.01956711296548153 66-67 0.021947902375730233 68-69 0.0276022772250709 70-71 0.026932680203438458 72-73 0.024254292116908664 74-75 0.025742285498314102 76-77 0.018674316936638267 78-79 0.014433535799632764 80-81 0.012275945396594877 82-83 0.008704761281221822 84-85 0.011829547382173244 86-87 0.009895155986346172 88-89 0.008109563928659644 90-91 0.008779160950292093 92-93 0.004761578820497407 94-95 0.0011903947051243517 96-97 5.207976834919038E-4 98-99 3.719983453513599E-4 100 0.1032667406695375 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 672046.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.145629051921304 #Duplication Level Percentage of deduplicated Percentage of total 1 77.37969523935452 39.57633188862747 2 11.207705268012761 11.464502723220898 3 4.328595967822084 6.641662910576117 4 2.1181820541724536 4.333430144285639 5 1.243927937026055 3.181073841722817 6 0.7777575107053988 2.386733828093042 7 0.5428698082184606 1.9435792484240335 8 0.3876030569321213 1.5859361735392812 9 0.31925623828227984 1.4695705022127554 >10 1.5733629128929 14.254493566270614 >50 0.07758484850150611 2.6835567184221314 >100 0.03441731982138152 3.056499486184262 >500 0.004958427431888839 1.915738513424476 >1k 0.003500066422509769 3.4434661349771982 >5k 5.833444037516282E-4 2.0634243200192612 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 8017 1.192924293872741 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 5815 0.8652681512872631 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 2516 0.3743791347616086 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 2435 0.36232638837222453 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2418 0.35979679962383526 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 2339 0.34804165191073233 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 2202 0.3276561425854778 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 2077 0.30905622531790977 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2073 0.3084610279653476 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 1692 0.2517684801338004 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 1638 0.24373331587421102 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 1309 0.19477833362597205 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1196 0.17796400841609059 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 1188 0.17677361371096623 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 971 0.14448415733446818 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 888 0.13213381226880302 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 870 0.12945542418227324 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 848 0.12618183874318126 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 841 0.12514024337619747 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 830 0.12350345065665147 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 824 0.12261065462780822 No Hit CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC 811 0.12067626323198115 No Hit CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 772 0.11487308904449993 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 745 0.11085550691470525 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 723 0.10758192147561328 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 721 0.10728432279933219 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 688 0.10237394464069423 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 7.439966907027198E-5 0.0 16-17 0.0 0.0 0.0 1.4879933814054396E-4 0.0 18-19 0.0 0.0 0.0 2.975986762810879E-4 1.4879933814054396E-4 20-21 7.439966907027198E-5 0.0 0.0 4.4639801442163183E-4 1.4879933814054396E-4 22-23 1.4879933814054396E-4 0.0 0.0 4.4639801442163183E-4 1.4879933814054396E-4 24-25 1.4879933814054396E-4 0.0 0.0 0.0013391940432648955 1.4879933814054396E-4 26-27 1.4879933814054396E-4 0.0 0.0 0.0019343913958270714 1.4879933814054396E-4 28-29 1.4879933814054396E-4 0.0 0.0 0.004389580475146046 1.4879933814054396E-4 30-31 1.4879933814054396E-4 0.0 0.0 0.01577272984289766 1.4879933814054396E-4 32-33 1.4879933814054396E-4 0.0 0.0 0.04553259747100645 1.4879933814054396E-4 34-35 1.4879933814054396E-4 0.0 0.0 0.09418998104296432 1.4879933814054396E-4 36-37 1.4879933814054396E-4 0.0 0.0 0.16129848254434964 1.4879933814054396E-4 38-39 1.4879933814054396E-4 0.0 0.0 0.2595804453861789 1.4879933814054396E-4 40-41 1.4879933814054396E-4 0.0 0.0 0.38591108346750075 1.4879933814054396E-4 42-43 1.4879933814054396E-4 0.0 0.0 0.49081461685658423 1.4879933814054396E-4 44-45 1.4879933814054396E-4 0.0 0.0 0.61550846221836 1.4879933814054396E-4 46-47 1.4879933814054396E-4 0.0 0.0 0.7696645765319636 1.4879933814054396E-4 48-49 1.4879933814054396E-4 0.0 0.0 0.912288742139675 1.4879933814054396E-4 50-51 1.4879933814054396E-4 0.0 0.0 1.061906476639992 1.4879933814054396E-4 52-53 1.4879933814054396E-4 0.0 0.0 1.2264785446234336 1.4879933814054396E-4 54-55 1.4879933814054396E-4 0.0 0.0 1.4068233424497727 1.4879933814054396E-4 56-57 1.4879933814054396E-4 0.0 0.0 1.6124640277600046 1.4879933814054396E-4 58-59 1.4879933814054396E-4 0.0 0.0 1.8408710118057394 1.4879933814054396E-4 60-61 1.4879933814054396E-4 0.0 0.0 2.0725515812905666 1.4879933814054396E-4 62-63 1.4879933814054396E-4 0.0 0.0 2.3102585239700852 1.4879933814054396E-4 64-65 1.4879933814054396E-4 0.0 0.0 2.58516530118474 1.4879933814054396E-4 66-67 2.2319900721081594E-4 0.0 0.0 2.8942215265026503 1.4879933814054396E-4 68-69 2.975986762810879E-4 0.0 0.0 3.236608803564042 1.4879933814054396E-4 70-71 2.975986762810879E-4 0.0 0.0 3.681518824604268 1.4879933814054396E-4 72-73 2.975986762810879E-4 0.0 0.0 4.095329783973121 1.4879933814054396E-4 74-75 2.975986762810879E-4 0.0 0.0 4.5206726920478655 1.4879933814054396E-4 76-77 2.975986762810879E-4 0.0 0.0 5.0038241429902115 1.4879933814054396E-4 78-79 2.975986762810879E-4 0.0 0.0 5.500441934034278 1.4879933814054396E-4 80-81 2.975986762810879E-4 0.0 0.0 6.056058662651068 1.4879933814054396E-4 82-83 2.975986762810879E-4 0.0 0.0 6.694482222943073 1.4879933814054396E-4 84-85 2.975986762810879E-4 0.0 0.0 7.34488412995539 1.4879933814054396E-4 86-87 2.975986762810879E-4 0.0 0.0 8.005255592623124 1.4879933814054396E-4 88 2.975986762810879E-4 0.0 0.0 8.549117173526811 1.4879933814054396E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATTAACG 90 6.760649E-4 27.68334 2 TAACGGT 95 0.0013005589 24.748123 4 TTAACGG 95 0.0013016897 24.744434 3 GTTATAG 105 0.0016561883 23.74171 1 AACGGTG 105 0.0023283134 22.391161 5 TACCGGA 115 0.0028191782 21.665224 2 GTCTTAC 145 4.430929E-4 20.630726 1 TTAAGAC 210 9.4877214E-7 20.149038 3 CTAGTAG 165 6.837225E-5 19.945515 3 CCAATCG 60 0.0051026205 19.557997 16-17 CAATCGT 60 0.0051026205 19.557997 16-17 GAGTACT 3400 0.0 18.849617 12-13 GACAGTA 200 1.4186106E-5 18.780571 7 CCTAGTA 160 8.629714E-4 18.686256 2 GTACTTT 3475 0.0 18.577902 14-15 ATACCTT 355 9.276846E-11 18.518812 6 ACACTGT 255 3.012683E-7 18.415068 6 CATGGGG 2155 0.0 18.328543 4 CTAGAGC 290 5.8520527E-8 17.833055 3 CCTAGAC 225 2.3423268E-5 17.717339 2 >>END_MODULE