FastQCFastQC Report
Wed 25 May 2016
SRR1295140_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295140_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2844483
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACT9700.019.5629164
CGTACAC2301.0690737E-519.4105593
GTACATG91950.018.4816021
TACATGG92850.017.6361582
ACATGGG95400.016.3204353
GTATTAG11900.015.5223171
GTATTAT13950.015.4481361
GTGTATA14800.015.3929641
CATGGGG37950.014.85374454
TAATACC13250.014.321534
GTATAGG7753.6379788E-1214.3005591
GTATAGA9550.014.1840931
CCTAGAC9450.014.1727893
GTACAGG13950.014.124011
GTATATA19450.013.61228751
TAGACTG13300.013.5171225
TACACTG17100.013.4336835
TACACCG3354.531671E-513.4162475
CATGGGT17300.013.2270844
AGTGTCG2700.00182698512.95612058
Produced by FastQC (version 0.11.5)