##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295140_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2844483 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.70908878696058 33.0 27.0 33.0 15.0 33.0 2 22.97290228136361 33.0 15.0 33.0 2.0 33.0 3 25.17679979103408 33.0 15.0 33.0 15.0 33.0 4 25.492012080929996 33.0 15.0 37.0 2.0 37.0 5 27.123673440832658 33.0 15.0 37.0 2.0 37.0 6 28.142173111950395 33.0 15.0 37.0 6.0 37.0 7 27.963437995586546 33.0 15.0 37.0 6.0 37.0 8 28.855791720323165 33.0 27.0 37.0 6.0 37.0 9 29.751563289356977 37.0 27.0 37.0 15.0 37.0 10-11 29.252423726912763 35.0 24.5 37.0 10.5 37.0 12-13 28.045228781469252 33.0 18.5 37.0 4.0 37.0 14-15 29.923628300819516 35.0 27.0 37.0 10.5 40.0 16-17 30.347049885691 37.0 27.0 40.0 10.5 40.0 18-19 30.46235185796505 37.0 27.0 40.0 10.5 40.0 20-21 29.99052938618371 35.0 27.0 40.0 6.0 40.0 22-23 30.708345242351598 37.0 27.0 40.0 15.0 40.0 24-25 30.832168446779256 37.0 27.0 40.0 15.0 40.0 26-27 29.427966874824 35.0 24.5 40.0 4.0 40.0 28-29 30.117523114042164 37.0 27.0 40.0 10.5 40.0 30-31 30.809566800012515 37.0 27.0 40.0 15.0 40.0 32-33 29.571996035834985 33.0 27.0 40.0 6.0 40.0 34-35 29.197053911027062 33.0 22.0 37.0 6.0 40.0 36-37 29.535447917952048 33.0 27.0 37.0 6.0 40.0 38-39 29.631809189930124 33.0 24.5 37.0 6.0 40.0 40-41 28.721454654501365 33.0 22.0 37.0 6.0 40.0 42-43 29.36722138961632 33.0 22.0 37.0 6.0 40.0 44-45 29.266837418258433 33.0 22.0 37.0 6.0 40.0 46-47 28.91855778361129 33.0 22.0 37.0 6.0 40.0 48-49 28.813063569021153 33.0 22.0 37.0 10.5 40.0 50-51 28.7999406218986 33.0 22.0 37.0 6.0 40.0 52-53 28.974358081943187 33.0 22.0 37.0 6.0 40.0 54-55 28.942306387487637 33.0 22.0 37.0 6.0 40.0 56-57 28.89768017597574 33.0 22.0 37.0 6.0 40.0 58-59 28.79109472617695 33.0 22.0 37.0 10.5 40.0 60-61 28.736045882503078 33.0 22.0 37.0 10.5 40.0 62-63 28.675975212367238 33.0 22.0 37.0 10.5 40.0 64-65 28.23311213320663 33.0 22.0 37.0 6.0 40.0 66-67 28.435957430577016 33.0 22.0 37.0 10.5 40.0 68-69 28.039546553802573 33.0 22.0 37.0 6.0 38.5 70-71 28.16072358316081 33.0 22.0 37.0 10.5 37.0 72-73 28.169207198636798 33.0 22.0 37.0 10.5 37.0 74-75 28.265017579644525 33.0 22.0 37.0 15.0 37.0 76-77 27.998162759278223 33.0 22.0 37.0 15.0 37.0 78-79 27.574287664928917 33.0 22.0 37.0 10.5 37.0 80-81 27.39972712088629 33.0 22.0 35.0 10.5 37.0 82-83 27.476010579075353 33.0 22.0 33.0 15.0 37.0 84-85 27.45373095919364 30.0 22.0 33.0 15.0 37.0 86-87 27.207720524256956 27.0 22.0 33.0 15.0 37.0 88-89 27.134931725730123 27.0 22.0 33.0 15.0 37.0 90-91 27.277590690469935 27.0 22.0 33.0 15.0 37.0 92-93 27.22331123089855 27.0 22.0 33.0 15.0 37.0 94-95 27.11234888730219 27.0 22.0 33.0 15.0 37.0 96-97 27.20248161089379 27.0 22.0 33.0 15.0 37.0 98-99 27.260729454174978 27.0 22.0 33.0 15.0 37.0 100 26.92139379985748 27.0 22.0 33.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.2287473418631905 1101 2 11.362345282675108 1101 3 8.41725356405954 1101 4 12.816455993303908 1101 5 10.662617303304227 1101 6 9.920353640158648 1101 7 9.602130743155012 1101 8 8.977477602770183 1101 9 7.309431308608026 1101 10-11 7.730186014389663 1101 12-13 9.044403875977455 1101 14-15 7.941376761388671 1101 16-17 8.235506628990446 1101 18-19 8.251655586669646 1101 20-21 7.2040084581799135 1101 22-23 7.356804019278339 1101 24-25 7.31196405786039 1101 26-27 8.646373706613655 1101 28-29 8.290761555117907 1101 30-31 5.466009402758068 1101 32-33 7.007879483261391 1101 34-35 8.68771802347731 1101 36-37 8.221967373899709 1101 38-39 8.248402431045598 1101 40-41 9.15709693831107 1101 42-43 8.655700501061883 1101 44-45 8.386785046892019 1101 46-47 7.29108043293602 1101 48-49 5.440025567202088 1101 50-51 6.834778825486058 1101 52-53 5.758419738569096 1101 54-55 3.921703597731362 1101 56-57 6.173018368702948 1101 58-59 6.204897729120404 1101 60-61 6.7553227923128425 1101 62-63 6.262890822069494 1101 64-65 6.706487484821043 1101 66-67 5.713044503101731 1101 68-69 6.7214455971702485 1101 70-71 6.31298449670259 1101 72-73 5.514653260409148 1101 74-75 4.917977370179472 1101 76-77 4.956730427202302 1101 78-79 5.063570234292079 1101 80-81 4.844604559609017 1101 82-83 4.521822390920573 1101 84-85 4.1688283409983775 1101 86-87 4.083575841340469 1101 88-89 4.028086283723532 1101 90-91 3.850293289952532 1101 92-93 3.8711083472326386 1101 94-95 3.668565377869516 1101 96-97 3.8082137367118634 1101 98-99 3.5217614966501003 1101 100 3.717609375042432 1102 1 6.789263447676834 1102 2 10.504901897012182 1102 3 8.207271037274133 1102 4 12.74171662060429 1102 5 10.68790199185526 1102 6 9.573582712651234 1102 7 6.792869763902559 1102 8 7.932322121532582 1102 9 6.541980815985781 1102 10-11 7.569745434804272 1102 12-13 9.112207915596638 1102 14-15 6.239182166233906 1102 16-17 7.382615497640824 1102 18-19 7.521769262151981 1102 20-21 7.116211755219908 1102 22-23 6.663900401719285 1102 24-25 7.050355382012892 1102 26-27 8.641441417323396 1102 28-29 7.7650057529262675 1102 30-31 4.226421006584275 1102 32-33 4.5670707322005555 1102 34-35 5.252100260215137 1102 36-37 5.3791058845151625 1102 38-39 4.8415028731453305 1102 40-41 6.3495843107332135 1102 42-43 7.058633386656009 1102 44-45 6.522639233118458 1102 46-47 5.494440316062292 1102 48-49 3.783398463092265 1102 50-51 5.164402429840038 1102 52-53 5.952498221286319 1102 54-55 5.56747936778487 1102 56-57 6.411919115242746 1102 58-59 6.17542437594436 1102 60-61 6.063353885278939 1102 62-63 4.37826150459934 1102 64-65 6.376847549646765 1102 66-67 4.080686753131626 1102 68-69 6.1411012045901465 1102 70-71 5.301013509396903 1102 72-73 5.143577760661248 1102 74-75 4.274627757155049 1102 76-77 3.4644969914045163 1102 78-79 4.463446928967631 1102 80-81 4.511784578743551 1102 82-83 3.871644396426195 1102 84-85 2.956300877175533 1102 86-87 3.260432176748658 1102 88-89 3.392363240089029 1102 90-91 3.3200305247646185 1102 92-93 3.2430380099317553 1102 94-95 3.2219206860767393 1102 96-97 3.337439838216156 1102 98-99 3.0776696402120507 1102 100 3.265056016948826 1103 1 6.597101455513904 1103 2 10.984360334364453 1103 3 7.891037441012173 1103 4 12.011792061195319 1103 5 10.207502246579576 1103 6 9.13813454240788 1103 7 7.948448734300623 1103 8 7.901259194468903 1103 9 7.128225546669512 1103 10-11 7.406298061551109 1103 12-13 8.76427497216341 1103 14-15 8.49936012164152 1103 16-17 7.940977565595961 1103 18-19 7.181137647426414 1103 20-21 8.384571908835852 1103 22-23 6.827702240266266 1103 24-25 5.2174751219753155 1103 26-27 5.946637285476218 1103 28-29 7.068102294223255 1103 30-31 6.090799217497569 1103 32-33 7.938263560851574 1103 34-35 7.178363704541077 1103 36-37 4.089833411540553 1103 38-39 6.424116766559614 1103 40-41 6.742415227701684 1103 42-43 7.616545037949361 1103 44-45 5.552031176485276 1103 46-47 6.477997431739169 1103 48-49 5.651481506344613 1103 50-51 6.518533730067023 1103 52-53 6.074693147896802 1103 54-55 4.871376845154646 1103 56-57 6.053275014337537 1103 58-59 5.5533927151183775 1103 60-61 4.953081306820334 1103 62-63 2.633990184135051 1103 64-65 5.020618797834981 1103 66-67 4.016796425232673 1103 68-69 5.03124313916663 1103 70-71 3.7104866754404355 1103 72-73 4.364598220649604 1103 74-75 3.170089835658583 1103 76-77 3.4712954349295586 1103 78-79 2.84151133671406 1103 80-81 4.102698851509537 1103 82-83 3.3409868013831634 1103 84-85 3.236871350251718 1103 86-87 2.705844052683851 1103 88-89 3.0226018292601182 1103 90-91 2.6215887577904873 1103 92-93 2.327968214687914 1103 94-95 2.8093130044977634 1103 96-97 2.999360701778464 1103 98-99 2.826036044731456 1103 100 2.787688506800876 1104 1 6.434240994256228 1104 2 9.89144843789855 1104 3 7.528153750950782 1104 4 12.30193973001894 1104 5 9.658180859488159 1104 6 8.339724056262202 1104 7 4.062047539676435 1104 8 6.650859327204923 1104 9 6.714115707611938 1104 10-11 7.197972194618966 1104 12-13 7.608366560018059 1104 14-15 7.445192210574657 1104 16-17 7.298141992342263 1104 18-19 7.07156572109491 1104 20-21 7.679357042401477 1104 22-23 6.698435606783601 1104 24-25 6.113089855673675 1104 26-27 8.49099600457362 1104 28-29 7.32693753563689 1104 30-31 6.497492431368485 1104 32-33 7.949833164302703 1104 34-35 8.31343422055236 1104 36-37 6.9106314868682475 1104 38-39 7.51995217389328 1104 40-41 7.534646852058742 1104 42-43 7.545040143308579 1104 44-45 6.91286575421881 1104 46-47 6.904383693560966 1104 48-49 5.693366357846191 1104 50-51 5.92067575760975 1104 52-53 6.157069122676965 1104 54-55 4.963248423054097 1104 56-57 6.293109094310985 1104 58-59 4.1126444157986555 1104 60-61 4.438115320425776 1104 62-63 4.093120763195948 1104 64-65 3.0216176363947973 1104 66-67 3.399447827256896 1104 68-69 4.305824191099596 1104 70-71 2.6883496254985033 1104 72-73 2.45357449408581 1104 74-75 3.2584190209912514 1104 76-77 3.8119541374349524 1104 78-79 4.406717907147115 1104 80-81 3.2219936913967118 1104 82-83 3.074285291510922 1104 84-85 3.0979730592279964 1104 86-87 0.8595955044840835 1104 88-89 0.3874533308363617 1104 90-91 2.3399809927332456 1104 92-93 2.3976197826321624 1104 94-95 2.469376054107851 1104 96-97 2.876206562073804 1104 98-99 2.60997664536195 1104 100 0.9473334379440637 1105 1 5.896166463216989 1105 2 -2.6567779297333765 1105 3 0.45288009250725736 1105 4 8.53888374832227 1105 5 8.725899952391618 1105 6 8.176124623945913 1105 7 5.465462678925402 1105 8 6.105033165293392 1105 9 5.0478103049745435 1105 10-11 4.962168180570014 1105 12-13 5.156176836586493 1105 14-15 5.832536533467238 1105 16-17 6.159055932779371 1105 18-19 6.206672362702246 1105 20-21 7.073169987084427 1105 22-23 2.132351252439644 1105 24-25 4.084991262868606 1105 26-27 6.99598492849066 1105 28-29 6.007821872971963 1105 30-31 5.349332692636175 1105 32-33 7.18873586730912 1105 34-35 6.254855091452022 1105 36-37 6.3230793026799645 1105 38-39 4.196178754970056 1105 40-41 5.5162986495315245 1105 42-43 5.8145001162519705 1105 44-45 6.268150126862654 1105 46-47 5.580360453245863 1105 48-49 5.061567129846335 1105 50-51 6.060436806951575 1105 52-53 5.685882295839512 1105 54-55 5.430562110584862 1105 56-57 5.287159991280994 1105 58-59 1.9396999528544008 1105 60-61 4.4779589075172375 1105 62-63 -0.8004300418308645 1105 64-65 -0.4590492513013409 1105 66-67 0.7927242416027482 1105 68-69 2.627041301233188 1105 70-71 2.8432551335189196 1105 72-73 2.6922139547093593 1105 74-75 2.6558475779498956 1105 76-77 1.6789145032583512 1105 78-79 3.249953363264332 1105 80-81 1.5757369900121745 1105 82-83 1.0203069399727802 1105 84-85 1.344306849278457 1105 86-87 -0.485951589571485 1105 88-89 1.4862737951741387 1105 90-91 1.3889210262663596 1105 92-93 1.705007476035302 1105 94-95 1.058707366815515 1105 96-97 1.9786108075393685 1105 98-99 1.8088943567478708 1105 100 1.9318970659881032 1106 1 6.233672082165203 1106 2 10.520453818539412 1106 3 6.814446116757562 1106 4 11.894959264902067 1106 5 9.9902567526203 1106 6 9.629108662563734 1106 7 6.625672589856443 1106 8 7.557215792221957 1106 9 7.0820407264799385 1106 10-11 5.90080582707235 1106 12-13 8.09092799722789 1106 14-15 8.005986020301666 1106 16-17 7.7545163853621695 1106 18-19 7.313086279738197 1106 20-21 8.270136976926267 1106 22-23 6.234483292551236 1106 24-25 5.124273657463075 1106 26-27 7.784514291536542 1106 28-29 6.439567433657675 1106 30-31 5.424697579721304 1106 32-33 7.5080649736197955 1106 34-35 7.019640213545308 1106 36-37 6.074322753058851 1106 38-39 7.239751689061194 1106 40-41 6.720893561858215 1106 42-43 6.2492685759851305 1106 44-45 4.5505180562431775 1106 46-47 6.1130731269512815 1106 48-49 6.054198615579356 1106 50-51 6.80313933880392 1106 52-53 5.7531275780463105 1106 54-55 5.355522245817909 1106 56-57 5.031740412491995 1106 58-59 5.100469413850654 1106 60-61 5.022358693904717 1106 62-63 5.070200719528742 1106 64-65 5.396511656377836 1106 66-67 3.2320073604664223 1106 68-69 3.363662720353762 1106 70-71 3.032573840574379 1106 72-73 2.878977802150338 1106 74-75 2.854423396351951 1106 76-77 3.6587435929212653 1106 78-79 4.350096138087633 1106 80-81 3.7629866579555866 1106 82-83 2.04412648668551 1106 84-85 2.719060278601951 1106 86-87 3.335096900564306 1106 88-89 3.4560616218136886 1106 90-91 3.2464211777636756 1106 92-93 2.9779730754886877 1106 94-95 1.4812410960062579 1106 96-97 1.5452933091276826 1106 98-99 2.0947405629241764 1106 100 2.9624996803706622 1107 1 6.080019448594854 1107 2 10.454886442536743 1107 3 6.108333094318052 1107 4 11.483924856467954 1107 5 9.550197835980885 1107 6 8.129800203169285 1107 7 3.5500145540345116 1107 8 6.294346297974581 1107 9 5.764897459822414 1107 10-11 4.524856342912667 1107 12-13 6.259441735475871 1107 14-15 6.231478360873407 1107 16-17 7.152152857407099 1107 18-19 6.378114764068034 1107 20-21 5.571491314128892 1107 22-23 5.838453552874011 1107 24-25 4.641905720429772 1107 26-27 7.384076487379151 1107 28-29 6.513889918038355 1107 30-31 5.024786034221634 1107 32-33 5.018789116762154 1107 34-35 6.884679724469834 1107 36-37 6.521854137133545 1107 38-39 6.586161791667799 1107 40-41 7.795015867536321 1107 42-43 6.285630058639452 1107 44-45 4.2675444051255695 1107 46-47 3.373536616679779 1107 48-49 3.977383606122526 1107 50-51 4.896003385258481 1107 52-53 4.060924947914952 1107 54-55 5.416941158003013 1107 56-57 5.343426294598515 1107 58-59 3.124838772955961 1107 60-61 2.8174505647778325 1107 62-63 0.21838571397099216 1107 64-65 2.035958971324053 1107 66-67 4.0596691328534895 1107 68-69 5.061621872357161 1107 70-71 4.285391587808334 1107 72-73 2.270069319610961 1107 74-75 2.524143002682248 1107 76-77 3.038976488013745 1107 78-79 3.825779767462418 1107 80-81 2.1162435038410656 1107 82-83 1.5125646919801206 1107 84-85 2.322091406819098 1107 86-87 2.259976508710011 1107 88-89 2.505325662903118 1107 90-91 1.3271711374861894 1107 92-93 2.2046336190421023 1107 94-95 2.199597537619656 1107 96-97 2.279950083363694 1107 98-99 1.7923089882469156 1107 100 -0.08907421395588244 1108 1 4.3059320427617465 1108 2 -3.2810504054755505 1108 3 -0.05657866483788965 1108 4 6.753673892197018 1108 5 7.001992715434248 1108 6 6.880880627900154 1108 7 4.209635637545098 1108 8 5.054388428614725 1108 9 4.896224879372038 1108 10-11 3.153592430968029 1108 12-13 2.392597553144906 1108 14-15 3.780726830274947 1108 16-17 4.62472100014476 1108 18-19 3.9683972443891697 1108 20-21 4.654278089418021 1108 22-23 4.084371200767993 1108 24-25 2.975893157774781 1108 26-27 4.759718010223832 1108 28-29 6.183253465773436 1108 30-31 4.593803594030597 1108 32-33 4.561412866054148 1108 34-35 3.0317203150869787 1108 36-37 1.2162881069948526 1108 38-39 2.4006260709280625 1108 40-41 5.414122935769463 1108 42-43 3.0957115156352977 1108 44-45 3.787611295191244 1108 46-47 4.8779359934524535 1108 48-49 4.887290070169268 1108 50-51 2.1588916986675493 1108 52-53 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48-49 -6.212480060543786 2213 50-51 -24.06040935936387 2213 52-53 -18.408810178045545 2213 54-55 -18.35494244531099 2213 56-57 -18.146710203696465 2213 58-59 -18.595395163669497 2213 60-61 -20.047509971819725 2213 62-63 -18.0995500967223 2213 64-65 -18.193660581322447 2213 66-67 -8.80814075141182 2213 68-69 -23.44355360880085 2213 70-71 -17.989055083613522 2213 72-73 -9.663605770481215 2213 74-75 -4.430176239729445 2213 76-77 -3.06059665671863 2213 78-79 -4.4550149012755895 2213 80-81 -3.1743817102051715 2213 82-83 -3.2480671527927427 2213 84-85 -2.99051963274902 2213 86-87 -3.16714929543145 2213 88-89 -2.7908941056992305 2213 90-91 -2.7258243981592543 2213 92-93 -3.7834804179802326 2213 94-95 -3.7041385845635837 2213 96-97 -3.2736991651838423 2213 98-99 -3.8189085228960415 2213 100 -3.2418606432360804 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 752.0 3 635.0 4 2859.0 5 12372.0 6 20635.0 7 23253.0 8 22007.0 9 21712.0 10 25703.0 11 31113.0 12 37583.0 13 50363.0 14 37087.0 15 40519.0 16 47552.0 17 51854.0 18 52524.0 19 52791.0 20 52239.0 21 51148.0 22 53194.0 23 59627.0 24 67241.0 25 75686.0 26 84525.0 27 91748.0 28 99919.0 29 105846.0 30 112437.0 31 123580.0 32 141927.0 33 173587.0 34 210865.0 35 246989.0 36 268088.0 37 226465.0 38 67952.0 39 106.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.18912604032187 20.65400184017114 13.851791354540019 24.30508076496697 2 14.921687186510932 21.096296268931717 40.443934231828834 23.538082312728523 3 18.09462619267605 26.38811644644582 29.7496987474472 25.76755861343093 4 12.118901565360602 17.430517952289538 39.15198821107999 31.29859227126987 5 13.046714458360578 37.66057555003518 35.87753033467717 13.415179656927071 6 30.536761179226175 37.98728915503692 18.804957058795225 12.670992606941683 7 26.030719852928264 31.7035249424165 24.631843504191743 17.633911700463493 8 23.706898041126326 36.91628954672275 21.908969830020553 17.46784258213037 9 24.522049574903697 17.16992795216897 22.178101882118796 36.12992059080854 10-11 23.094926531137272 27.342853812561135 29.45750016880505 20.104719487496546 12-13 24.0399093483756 24.937781883918703 29.29942059836706 21.72288816933864 14-15 21.456671681189864 26.518198934650357 26.593643855316813 25.431485528842966 16-17 21.079311608166684 29.983984469788886 27.406388131569052 21.530315790475374 18-19 21.039241381724903 29.117009618255146 28.956203415047156 20.8875455849728 20-21 22.433433338243695 28.58153423908828 27.861845722336493 21.123186700331534 22-23 21.500082994981135 28.569241127463506 28.307014288192146 21.623661589363213 24-25 21.74919793659143 28.436214847202418 27.8869549949907 21.927632221215454 26-27 21.64787959509029 28.3370239974666 28.05886589888434 21.95623050855877 28-29 21.509624608707977 28.275790912194232 28.430425811482117 21.784158667615674 30-31 22.039229761814976 28.727369615348834 27.801978388454025 21.431422234382165 32-33 21.233078986246557 28.520824286956305 28.34615265481065 21.89994407198649 34-35 21.363760285773907 28.0830266969907 28.240701062533002 22.31251195470239 36-37 21.950473273359545 28.605350286003734 27.784881606725868 21.659294833910845 38-39 21.73711912823178 28.351464942117904 27.962199891660983 21.949216037989334 40-41 21.788556394725433 28.322395260698553 27.80167058728417 22.08737775729184 42-43 21.61306474569553 28.366084780167316 28.22264627987004 21.798204194267115 44-45 21.95036019880508 28.025230501560976 28.17770195017654 21.8467073494574 46-47 21.80827919650702 28.402378903092046 28.246067625253886 21.54327427514705 48-49 21.731409082130128 28.173448903507488 28.28203370536629 21.813108308996096 50-51 21.878112643181584 28.255430999825993 28.05993450698732 21.8065218500051 52-53 21.423747705580535 28.231184106092705 28.203299993713664 22.14176819461309 54-55 21.864386422011567 28.288633885839936 27.924330616996507 21.922649075151984 56-57 21.982727693018955 28.165714285714287 27.868811835413776 21.98274618585298 58-59 21.636583337153787 27.779578847940922 28.28831749483147 22.29552032007382 60-61 22.023959993405505 28.206297741385946 27.86113095565203 21.90861130955652 62-63 21.588171012360394 28.31127852785852 28.155453442469568 21.945097017311518 64-65 21.59426975921145 27.824677362041463 28.269840548365377 22.31121233038171 66-67 21.70797883477379 28.075403010311256 27.950589006061055 22.2660291488539 68-69 22.204309859800542 27.972131633560625 27.879973945953378 21.94358456068545 70-71 22.10594657564131 28.003689205330485 27.77710979129782 22.113254427730382 72-73 21.821740413832195 28.121918129456496 28.14194192243404 21.91439953427727 74-75 22.076699459363645 27.780058495081093 27.82469201453514 22.318550031020116 76-77 21.98184361840889 28.086345563379645 27.93128523218928 22.00052558602219 78-79 21.94312825033559 27.926339560430126 28.12440073614645 22.00613145308784 80-81 22.07381288140244 27.824539659388982 27.985311623401778 22.116335835806805 82-83 21.616165481535102 28.003881994592632 28.26346115108793 22.116491372784335 84-85 21.804438548981047 27.715575106998514 27.92096601830399 22.55902032571645 86-87 22.03078960518945 27.972294976515638 27.840886492873025 22.156028925421886 88-89 21.784184497654866 27.55939390116055 28.236566243240503 22.41985535794408 90-91 22.014316567130354 27.62904008116346 27.97064531866329 22.385998033042902 92-93 21.685166256381414 27.902496183235737 28.17980479195768 22.232532768425173 94-95 21.589966645712018 27.46316946656815 28.21264268507849 22.734221202641347 96-97 21.779893322697514 27.664914500422423 28.112456112677968 22.442736064202094 98-99 21.751613418770138 27.525732142021468 28.138235265550758 22.584419173657633 100 21.8819724204782 27.344464242566403 27.985626657660955 22.787936679294447 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 287.0 1 387.0 2 384.0 3 284.5 4 317.5 5 414.5 6 585.5 7 847.0 8 1244.5 9 1830.5 10 2583.0 11 3534.0 12 4523.0 13 5475.5 14 6399.0 15 7320.5 16 8333.5 17 9274.5 18 10080.5 19 10804.5 20 11517.0 21 12427.0 22 13466.5 23 14737.5 24 16383.5 25 18592.5 26 22054.0 27 26934.5 28 33196.5 29 40544.0 30 48790.5 31 57797.5 32 67073.5 33 75921.5 34 84323.5 35 91827.5 36 98272.0 37 103771.0 38 107774.0 39 110262.5 40 111591.0 41 111154.0 42 109063.5 43 105780.5 44 101498.0 45 97213.5 46 92947.5 47 89036.5 48 85691.5 49 83360.0 50 82314.5 51 78192.5 52 70197.5 53 63145.0 54 57937.0 55 53608.0 56 49436.0 57 44782.0 58 39892.5 59 34940.0 60 30041.5 61 25299.0 62 20826.5 63 16934.5 64 13580.5 65 10921.5 66 8683.0 67 6657.0 68 5010.0 69 3754.0 70 2832.5 71 2134.0 72 1604.0 73 1150.5 74 846.5 75 637.5 76 415.5 77 279.0 78 191.5 79 134.0 80 97.0 81 65.5 82 47.5 83 37.0 84 26.5 85 22.0 86 12.0 87 6.0 88 4.0 89 2.0 90 2.0 91 1.0 92 1.0 93 1.0 94 0.5 95 1.0 96 1.5 97 3.5 98 2.5 99 0.0 100 1.5 >>END_MODULE >>Per base N content fail #Base N-Count 1 7.495421839399287 2 23.86855537544081 3 5.9408686921314 4 23.324168223188536 5 12.526845827519448 6 9.403149886991766 7 7.90333427902364 8 8.432956006416632 9 5.249776497170136 10-11 6.80250154421735 12-13 9.91130901467859 14-15 7.107020854053267 16-17 7.640949163696882 18-19 6.615613452427032 20-21 7.761638934034762 22-23 5.751414228877445 24-25 5.290732973267901 26-27 9.41158727262564 28-29 6.12844232150447 30-31 4.2077769492733825 32-33 8.63448647785907 34-35 7.090726153047848 36-37 6.755445541421763 38-39 5.992987126307312 40-41 8.246226115606948 42-43 6.337707063111293 44-45 6.202972561270361 46-47 5.8307080759491265 48-49 3.8908652292877126 50-51 6.255583176274914 52-53 4.36972201978356 54-55 4.873750344087132 56-57 4.9475774683835345 58-59 3.37906747904628 60-61 4.0405585127420345 62-63 3.134137205249601 64-65 4.4784764050268535 66-67 2.4452246682437546 68-69 4.898500008613165 70-71 2.3430971463004 72-73 1.505229597083196 74-75 0.8343519718697562 76-77 1.2053684272326466 78-79 1.7362381845839825 80-81 2.1130201867966867 82-83 0.7812491760365592 84-85 1.1435469995777792 86-87 0.5589240645839684 88-89 0.8049617452450938 90-91 0.41065458995536275 92-93 0.3612607282237229 94-95 0.5119383733353302 96-97 0.4850266287406183 98-99 0.46231599907610627 100 0.48117707154516304 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2844483.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 94.78454047652211 #Duplication Level Percentage of deduplicated Percentage of total 1 96.52938161686136 91.49493079037045 2 2.7677082411946943 5.246719076294446 3 0.4250867598102241 1.2087495957379746 4 0.12621619603515863 0.47853376567548567 5 0.056218516453914485 0.2664323124178041 6 0.029368513108177587 0.16702086116623974 7 0.017630964645462932 0.11698000174546082 8 0.01125382878398634 0.08533511919132823 9 0.007364750896275105 0.06282580764847412 >10 0.027834244418800783 0.3980519393501756 >50 7.025553279123437E-4 0.043840033196349194 >100 0.0010475842300816975 0.22091549630493054 >500 1.1173694036148379E-4 0.07272441721868288 >1k 7.449129357271296E-5 0.13694078368219734 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 3.515577347447673E-5 0.0 2 0.0 0.0 0.0 3.515577347447673E-5 0.0 3 0.0 0.0 0.0 3.515577347447673E-5 0.0 4 0.0 0.0 0.0 7.031154694895347E-5 0.0 5 0.0 3.515577347447673E-5 0.0 7.031154694895347E-5 0.0 6 0.0 3.515577347447673E-5 0.0 7.031154694895347E-5 0.0 7 0.0 3.515577347447673E-5 0.0 7.031154694895347E-5 0.0 8 0.0 3.515577347447673E-5 0.0 7.031154694895347E-5 0.0 9 0.0 3.515577347447673E-5 0.0 7.031154694895347E-5 0.0 10-11 3.515577347447673E-5 3.515577347447673E-5 0.0 7.031154694895347E-5 0.0 12-13 3.515577347447673E-5 3.515577347447673E-5 0.0 7.031154694895347E-5 0.0 14-15 3.515577347447673E-5 3.515577347447673E-5 0.0 7.031154694895347E-5 0.0 16-17 3.515577347447673E-5 5.27336602117151E-5 0.0 8.788943368619183E-5 0.0 18-19 3.515577347447673E-5 7.031154694895347E-5 0.0 1.2304520716066857E-4 0.0 20-21 3.515577347447673E-5 7.031154694895347E-5 0.0 1.7577886737238366E-4 0.0 22-23 7.031154694895347E-5 7.031154694895347E-5 0.0 2.6366830105857553E-4 0.0 24-25 7.031154694895347E-5 8.788943368619183E-5 0.0 3.33979848007529E-4 0.0 26-27 7.031154694895347E-5 1.0546732042343019E-4 0.0 5.449144888543893E-4 0.0 28-29 7.031154694895347E-5 1.0546732042343019E-4 0.0 8.788943368619183E-4 0.0 30-31 7.031154694895347E-5 1.0546732042343019E-4 0.0 0.001863255994147267 0.0 32-33 7.031154694895347E-5 1.0546732042343019E-4 0.0 0.005923747830449329 0.0 34-35 7.031154694895347E-5 1.0546732042343019E-4 0.0 0.012550611130388193 0.0 36-37 7.031154694895347E-5 1.0546732042343019E-4 0.0 0.022253604609343773 0.0 38-39 1.0546732042343019E-4 1.0546732042343019E-4 0.0 0.037985813239172106 1.7577886737238366E-5 40-41 1.0546732042343019E-4 1.0546732042343019E-4 0.0 0.05979997068008492 3.515577347447673E-5 42-43 1.0546732042343019E-4 1.0546732042343019E-4 0.0 0.08289731385281614 3.515577347447673E-5 44-45 1.0546732042343019E-4 1.0546732042343019E-4 0.0 0.10959812380668121 3.515577347447673E-5 46-47 1.0546732042343019E-4 1.0546732042343019E-4 0.0 0.14195901328993704 3.515577347447673E-5 48-49 1.0546732042343019E-4 1.0546732042343019E-4 0.0 0.17785305800737777 3.515577347447673E-5 50-51 1.0546732042343019E-4 1.0546732042343019E-4 0.0 0.21687596656404695 3.515577347447673E-5 52-53 1.0546732042343019E-4 1.4062309389790693E-4 0.0 0.2583773571506668 3.515577347447673E-5 54-55 1.4062309389790693E-4 1.4062309389790693E-4 0.0 0.3063474100565903 3.515577347447673E-5 56-57 1.9335675410962202E-4 1.7577886737238366E-4 0.0 0.3622626677677455 3.515577347447673E-5 58-59 2.1093464084686038E-4 1.7577886737238366E-4 0.0 0.4247344772318907 3.515577347447673E-5 60-61 2.1093464084686038E-4 1.9335675410962202E-4 0.0 0.4910031102312793 5.27336602117151E-5 62-63 2.2851252758409875E-4 2.1093464084686038E-4 0.0 0.5615607475945541 7.031154694895347E-5 64-65 2.4609041432133714E-4 2.4609041432133714E-4 0.0 0.6416455995694121 7.031154694895347E-5 66-67 2.4609041432133714E-4 2.8124618779581386E-4 0.0 0.7385876449252817 7.031154694895347E-5 68-69 2.4609041432133714E-4 2.8124618779581386E-4 0.0 0.846427980058239 7.031154694895347E-5 70-71 2.4609041432133714E-4 2.988240745330522E-4 1.7577886737238366E-5 0.9740082819971152 7.031154694895347E-5 72-73 2.6366830105857553E-4 3.164019612702906E-4 3.515577347447673E-5 1.1087779396115218 7.031154694895347E-5 74-75 2.8124618779581386E-4 3.164019612702906E-4 3.515577347447673E-5 1.250543596147349 7.031154694895347E-5 76-77 2.8124618779581386E-4 3.164019612702906E-4 3.515577347447673E-5 1.407461391050676 7.031154694895347E-5 78-79 2.8124618779581386E-4 3.164019612702906E-4 3.515577347447673E-5 1.5844882883814035 7.031154694895347E-5 80-81 2.8124618779581386E-4 3.164019612702906E-4 3.515577347447673E-5 1.7892530909834932 7.031154694895347E-5 82-83 2.8124618779581386E-4 3.164019612702906E-4 3.515577347447673E-5 2.013177790129173 7.031154694895347E-5 84-85 2.988240745330522E-4 3.6913562148200565E-4 3.515577347447673E-5 2.239651282851752 7.031154694895347E-5 86-87 3.164019612702906E-4 3.8671350821924404E-4 3.515577347447673E-5 2.489643988028756 7.031154694895347E-5 88 3.164019612702906E-4 3.8671350821924404E-4 3.515577347447673E-5 2.699822779745915 7.031154694895347E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTAGACT 970 0.0 19.562916 4 CGTACAC 230 1.0690737E-5 19.410559 3 GTACATG 9195 0.0 18.481602 1 TACATGG 9285 0.0 17.636158 2 ACATGGG 9540 0.0 16.320435 3 GTATTAG 1190 0.0 15.522317 1 GTATTAT 1395 0.0 15.448136 1 GTGTATA 1480 0.0 15.392964 1 CATGGGG 3795 0.0 14.8537445 4 TAATACC 1325 0.0 14.32153 4 GTATAGG 775 3.6379788E-12 14.300559 1 GTATAGA 955 0.0 14.184093 1 CCTAGAC 945 0.0 14.172789 3 GTACAGG 1395 0.0 14.12401 1 GTATATA 1945 0.0 13.6122875 1 TAGACTG 1330 0.0 13.517122 5 TACACTG 1710 0.0 13.433683 5 TACACCG 335 4.531671E-5 13.416247 5 CATGGGT 1730 0.0 13.227084 4 AGTGTCG 270 0.001826985 12.9561205 8 >>END_MODULE