FastQCFastQC Report
Wed 25 May 2016
SRR1295140_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295140_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2844483
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT56260.1977863815674061No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA43450.15275183574660142No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA86000.017.0462911
CATGGGG44050.016.5470094
TATACCG2002.9818856E-416.4589355
GTACATG112150.016.0847261
ACATGGG113850.015.5297893
TACATGG115850.015.5162792
TCTAGAC10950.015.4596313
ATCAACG93200.015.1866273
CAACGCA92000.015.1810685
AACGCAG93350.015.0408166
TATCAAC95300.015.0063352
TCAACGC93450.014.9958344
GTATAGA11400.014.8210981
CTAGACT12700.014.8111914
CTAGTAC7950.014.7871223
GAGTACT28750.014.45328212-13
GTCCTAC7200.013.8039651
ACGCAGA101400.013.7994677
CGCAGAG100950.013.721448
GTATAAG10650.013.5317731
Produced by FastQC (version 0.11.5)