FastQCFastQC Report
Wed 25 May 2016
SRR1295139_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295139_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2597947
Sequences flagged as poor quality0
Sequence length100
%GC43

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA16150.019.1685351
GTACATG67300.017.8475281
CGGTACT1900.001222199517.7448794
TACATGG68650.017.1314492
CTGTCGC5450.016.5781579
GTATAAG10200.016.38911
TAATACG2453.6028665E-416.054894
GTATTAA14150.015.7520671
CCTATAC6800.015.6999333
ACATGGG72450.015.43361953
TACTGCG2906.222285E-414.9410492
GTATAAA17050.014.5253661
GTGTATA12500.014.3641351
TCTGTCG4552.048182E-814.3364968
GCACCGT2458.560259E-414.3253416
ATACCGT2458.560259E-414.3253416
GTATCAA43800.014.2770591
CGCAGCG3202.8151004E-514.1124888
TATACAG14100.013.8308065
TCAACGC45150.013.8146734
Produced by FastQC (version 0.11.5)