##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295139_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2597947
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.575986731061104	33.0	27.0	33.0	15.0	33.0
2	22.808924508467648	33.0	6.0	33.0	2.0	33.0
3	25.112150863739714	33.0	15.0	33.0	15.0	33.0
4	25.342964271403535	33.0	6.0	37.0	2.0	37.0
5	27.10218029852033	33.0	15.0	37.0	2.0	37.0
6	27.98155081685654	33.0	15.0	37.0	6.0	37.0
7	27.91622692841694	33.0	15.0	37.0	6.0	37.0
8	28.57481311204578	33.0	22.0	37.0	6.0	37.0
9	29.630800397390708	37.0	27.0	37.0	15.0	37.0
10-11	29.145277405582178	35.0	24.5	37.0	10.5	37.0
12-13	27.95988467047249	33.0	15.0	37.0	4.0	37.0
14-15	29.758399035854083	33.0	27.0	37.0	10.5	40.0
16-17	30.203332862448697	37.0	27.0	40.0	10.5	40.0
18-19	30.348680900726613	37.0	27.0	40.0	10.5	40.0
20-21	29.88129492249072	35.0	27.0	40.0	6.0	40.0
22-23	30.611147571524747	37.0	27.0	40.0	15.0	40.0
24-25	30.755746171881107	37.0	27.0	40.0	15.0	40.0
26-27	29.3217888971561	33.0	24.5	40.0	4.0	40.0
28-29	30.017642584702457	35.0	27.0	40.0	10.5	40.0
30-31	30.692426943274825	37.0	27.0	40.0	15.0	40.0
32-33	29.50649262667791	33.0	27.0	40.0	6.0	40.0
34-35	29.11914369307765	33.0	22.0	37.0	6.0	40.0
36-37	29.445346845028016	33.0	27.0	37.0	6.0	40.0
38-39	29.578289125990636	33.0	24.5	37.0	6.0	40.0
40-41	28.62638845211238	33.0	22.0	37.0	6.0	40.0
42-43	29.28562880613038	33.0	22.0	37.0	6.0	40.0
44-45	29.19782562923724	33.0	22.0	37.0	6.0	40.0
46-47	28.890192717557362	33.0	22.0	37.0	6.0	40.0
48-49	28.83761620233207	33.0	22.0	37.0	6.0	40.0
50-51	28.79088834375759	33.0	22.0	37.0	6.0	40.0
52-53	28.979064815410013	33.0	22.0	37.0	6.0	40.0
54-55	28.981599316691216	33.0	22.0	37.0	6.0	40.0
56-57	28.97221036456864	33.0	22.0	37.0	6.0	40.0
58-59	28.83706846213568	33.0	22.0	37.0	10.5	40.0
60-61	28.778082270346545	33.0	22.0	37.0	10.5	40.0
62-63	28.715946861117644	33.0	22.0	37.0	10.5	40.0
64-65	28.31220844767041	33.0	22.0	37.0	6.0	40.0
66-67	28.52500435921133	33.0	22.0	37.0	10.5	40.0
68-69	28.100269366542122	33.0	22.0	37.0	6.0	40.0
70-71	28.236555441662205	33.0	22.0	37.0	10.5	37.0
72-73	28.267504302435732	33.0	22.0	37.0	10.5	37.0
74-75	28.35504823616494	33.0	22.0	37.0	15.0	37.0
76-77	28.141304460791538	33.0	22.0	37.0	15.0	37.0
78-79	27.678411453351433	33.0	22.0	37.0	10.5	37.0
80-81	27.482111836769572	33.0	22.0	37.0	10.5	37.0
82-83	27.57339025776892	33.0	22.0	33.0	15.0	37.0
84-85	27.525686051332073	30.0	22.0	33.0	15.0	37.0
86-87	27.309831570851905	27.0	22.0	33.0	15.0	37.0
88-89	27.252758813016584	27.0	22.0	33.0	15.0	37.0
90-91	27.389189232882735	27.0	22.0	33.0	15.0	37.0
92-93	27.32183123828161	27.0	22.0	33.0	15.0	37.0
94-95	27.194544192010078	27.0	22.0	33.0	15.0	37.0
96-97	27.281743430485687	27.0	22.0	33.0	15.0	37.0
98-99	27.35009143758514	27.0	22.0	33.0	15.0	37.0
100	27.031164223134653	27.0	22.0	33.0	15.0	37.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	7.31070377491411
1101	2	11.303531967406798
1101	3	8.424491738316565
1101	4	12.917215651730611
1101	5	10.79810386080884
1101	6	10.026539261591743
1101	7	9.43144089323091
1101	8	8.958999922693824
1101	9	7.307340082406679
1101	10-11	7.781664796305275
1101	12-13	9.242339750675459
1101	14-15	8.133866440244997
1101	16-17	8.282174202015618
1101	18-19	8.189314527817103
1101	20-21	7.318858984083729
1101	22-23	7.548969744433496
1101	24-25	7.401462038852234
1101	26-27	8.591380981959915
1101	28-29	8.281359060191711
1101	30-31	5.5911353428590225
1101	32-33	7.093843853388453
1101	34-35	8.835991626268086
1101	36-37	8.208502266190632
1101	38-39	8.227575546928307
1101	40-41	9.007037368357867
1101	42-43	8.672721060270753
1101	44-45	8.463198737003815
1101	46-47	7.394449881561524
1101	48-49	5.564572365086132
1101	50-51	6.8415353047629495
1101	52-53	5.773767214129791
1101	54-55	4.039540339846457
1101	56-57	6.280399535300745
1101	58-59	6.386712462340395
1101	60-61	6.921250646872892
1101	62-63	6.476882677780605
1101	64-65	6.958802934157056
1101	66-67	6.058419642362061
1101	68-69	6.836729910841456
1101	70-71	6.322519336874834
1101	72-73	5.440907224880757
1101	74-75	4.983700502918115
1101	76-77	5.237216659078296
1101	78-79	5.310703785775711
1101	80-81	5.121846642767149
1101	82-83	4.654516848216073
1101	84-85	4.409732236456033
1101	86-87	4.1877680411338005
1101	88-89	4.156539541995361
1101	90-91	3.957562624156161
1101	92-93	3.876343092775297
1101	94-95	3.801619859138956
1101	96-97	4.022479718648427
1101	98-99	3.680820272551898
1101	100	3.887200643833257
1102	1	6.766797822103232
1102	2	10.678426084984178
1102	3	8.165021112242954
1102	4	12.76869640024643
1102	5	10.66528870308132
1102	6	9.520896308670359
1102	7	6.719600251245122
1102	8	7.775653135685477
1102	9	6.4657745702830205
1102	10-11	7.405607074818011
1102	12-13	9.093738841956213
1102	14-15	6.255090400993414
1102	16-17	7.411204510269734
1102	18-19	7.478106631987984
1102	20-21	7.053828948280181
1102	22-23	6.638127661795249
1102	24-25	6.959752742038081
1102	26-27	8.529043920407752
1102	28-29	7.64674236212101
1102	30-31	4.23013213564214
1102	32-33	4.509553417977237
1102	34-35	5.165874792050044
1102	36-37	5.200322109662483
1102	38-39	4.716984327660633
1102	40-41	6.159225958384159
1102	42-43	7.041272971531676
1102	44-45	6.552600971441279
1102	46-47	5.611625130394767
1102	48-49	3.7487324201248846
1102	50-51	5.1824176486731695
1102	52-53	5.951497856852804
1102	54-55	5.452518462908383
1102	56-57	6.418141434533631
1102	58-59	6.250269332419673
1102	60-61	6.22767899931231
1102	62-63	4.3755040904305105
1102	64-65	6.22717052045865
1102	66-67	3.901956243865559
1102	68-69	6.087459129121889
1102	70-71	5.298313834963388
1102	72-73	5.025502078588996
1102	74-75	4.344873913801141
1102	76-77	3.5028241716062674
1102	78-79	4.493597475661829
1102	80-81	4.598064202953704
1102	82-83	3.986725398207085
1102	84-85	3.090068678604009
1102	86-87	3.301418608954741
1102	88-89	3.495625302042793
1102	90-91	3.441916013102869
1102	92-93	3.258102325350034
1102	94-95	3.1751420291795895
1102	96-97	3.349907228953775
1102	98-99	3.1979139888413997
1102	100	3.2701337050069768
1103	1	6.775243021738632
1103	2	11.112834735758646
1103	3	8.023200791703534
1103	4	12.362326715399956
1103	5	10.641285043407198
1103	6	9.326743167547185
1103	7	8.42933994089989
1103	8	8.241267595375607
1103	9	7.263315310314422
1103	10-11	7.490231655485626
1103	12-13	8.964482976116226
1103	14-15	8.813876228230246
1103	16-17	8.309767034938211
1103	18-19	7.565520016829339
1103	20-21	8.621359274352425
1103	22-23	7.224526108371766
1103	24-25	5.504565557408203
1103	26-27	6.1644010217360865
1103	28-29	7.293477044740676
1103	30-31	6.297845543587631
1103	32-33	8.27103815987724
1103	34-35	7.514267199252249
1103	36-37	4.498627746538499
1103	38-39	6.72970938077227
1103	40-41	7.159823448444566
1103	42-43	7.896797085075306
1103	44-45	6.083930835228241
1103	46-47	6.823179647024414
1103	48-49	5.93734299226627
1103	50-51	6.888885963079865
1103	52-53	6.464114625182724
1103	54-55	5.367841584443713
1103	56-57	6.356101189645038
1103	58-59	5.903326459284148
1103	60-61	5.456791848173289
1103	62-63	3.1845968960776467
1103	64-65	5.4768877038522525
1103	66-67	4.625530513767416
1103	68-69	5.595607388737335
1103	70-71	4.234554048641137
1103	72-73	4.952437306081084
1103	74-75	3.663360641972428
1103	76-77	4.069813817940791
1103	78-79	3.50854357805569
1103	80-81	4.628126933710018
1103	82-83	3.8824246382787706
1103	84-85	3.731327181785641
1103	86-87	3.2194199540357147
1103	88-89	3.4058608438473357
1103	90-91	3.0808508347526598
1103	92-93	2.7194845585011187
1103	94-95	3.2757878491811816
1103	96-97	3.4899607294576604
1103	98-99	3.1590021636555647
1103	100	3.3327649324190176
1104	1	6.546361921850245
1104	2	10.035200625755454
1104	3	7.610211149779662
1104	4	12.602564574069522
1104	5	9.746059404637382
1104	6	8.455161285246838
1104	7	4.462145674229742
1104	8	6.941307604204081
1104	9	6.758539436656957
1104	10-11	7.388899924812531
1104	12-13	7.72448867591633
1104	14-15	7.570492328556977
1104	16-17	7.461619460358882
1104	18-19	7.1266270456685135
1104	20-21	7.873246427923267
1104	22-23	6.874100376113429
1104	24-25	6.255278048442587
1104	26-27	8.438152682842663
1104	28-29	7.4690665997896915
1104	30-31	6.610922925519613
1104	32-33	7.890203010349449
1104	34-35	8.30902078767824
1104	36-37	6.928175631396126
1104	38-39	7.607331567593441
1104	40-41	7.456687674002335
1104	42-43	7.6140549409814895
1104	44-45	7.003077898658418
1104	46-47	7.014577671999465
1104	48-49	5.761139942879627
1104	50-51	5.850152540180069
1104	52-53	6.280079076989352
1104	54-55	5.182807337578669
1104	56-57	6.3682021299390215
1104	58-59	4.222506212932817
1104	60-61	4.65823125277425
1104	62-63	4.129986287143662
1104	64-65	3.1310413738980394
1104	66-67	3.530073794052999
1104	68-69	4.404027712273731
1104	70-71	2.8873663206555378
1104	72-73	2.75905830577501
1104	74-75	3.4538874287258174
1104	76-77	4.059165320524478
1104	78-79	4.6627885224470695
1104	80-81	3.3544057935141574
1104	82-83	3.137443862126723
1104	84-85	3.1439341187256247
1104	86-87	0.9812485933330848
1104	88-89	0.512200663442961
1104	90-91	2.5672283669788953
1104	92-93	2.6873188350507533
1104	94-95	2.652235966260484
1104	96-97	3.1238646644345636
1104	98-99	2.756208508595627
1104	100	1.2114597089566033
1105	1	5.838209237368783
1105	2	-2.9572635781921264
1105	3	0.3871604886550344
1105	4	8.650972571668763
1105	5	8.595213097745088
1105	6	8.172974160294572
1105	7	5.499605475304772
1105	8	6.042198108396285
1105	9	5.172473319882268
1105	10-11	5.103489526940223
1105	12-13	5.1550696738750545
1105	14-15	6.013786821583462
1105	16-17	6.207651842146781
1105	18-19	6.346335702358147
1105	20-21	7.212269445692929
1105	22-23	2.299730067284738
1105	24-25	4.230094032424379
1105	26-27	7.088451868430383
1105	28-29	6.055290690263341
1105	30-31	5.392832599640261
1105	32-33	7.222369084568822
1105	34-35	6.327574527928437
1105	36-37	6.469269297610378
1105	38-39	4.401118477898578
1105	40-41	5.815069083198399
1105	42-43	5.960949898715825
1105	44-45	6.319819055235314
1105	46-47	5.672694999613544
1105	48-49	5.201728329909802
1105	50-51	6.185721451513714
1105	52-53	5.767274049243781
1105	54-55	5.577894452795764
1105	56-57	5.344548591514574
1105	58-59	2.0030025686938657
1105	60-61	4.601697545845383
1105	62-63	-0.9390519960138697
1105	64-65	-0.38830050047529596
1105	66-67	0.9861161940252252
1105	68-69	2.963575789492623
1105	70-71	3.1341890410015374
1105	72-73	2.880498941622232
1105	74-75	2.8581468249758224
1105	76-77	1.9835328592213095
1105	78-79	3.381223550633205
1105	80-81	1.6739945451513982
1105	82-83	1.036749309509176
1105	84-85	1.5426810336108048
1105	86-87	-0.12219412784476091
1105	88-89	1.749802459305343
1105	90-91	1.5523355772415002
1105	92-93	2.024677942075648
1105	94-95	1.249821973830059
1105	96-97	2.2831473925213714
1105	98-99	2.1438420240077214
1105	100	2.203043143885754
1106	1	6.348363168222537
1106	2	10.485832569197264
1106	3	6.992251658962324
1106	4	12.085338628950606
1106	5	10.158471849707983
1106	6	9.50207141808692
1106	7	6.680118881872513
1106	8	7.44096610973352
1106	9	7.031934355758938
1106	10-11	5.897898466758857
1106	12-13	8.064322545290171
1106	14-15	7.9766303996219925
1106	16-17	7.790586703586236
1106	18-19	7.461831566580344
1106	20-21	8.48472838829478
1106	22-23	6.376893961446424
1106	24-25	5.365346182286107
1106	26-27	8.02529945656039
1106	28-29	6.46391564340167
1106	30-31	5.621398590092593
1106	32-33	7.636165464385666
1106	34-35	7.07816518947482
1106	36-37	6.1583497309209925
1106	38-39	7.294893275280515
1106	40-41	6.845623211492768
1106	42-43	6.506111729279752
1106	44-45	4.886148563865163
1106	46-47	6.327188578381975
1106	48-49	6.2106811743589
1106	50-51	6.772856738602286
1106	52-53	5.908535031958422
1106	54-55	5.448095035973882
1106	56-57	5.063406817503875
1106	58-59	5.137791291350272
1106	60-61	5.0510623366390135
1106	62-63	5.085564428100387
1106	64-65	5.34954155761697
1106	66-67	3.183338887248091
1106	68-69	3.6087181294527078
1106	70-71	3.079929413645843
1106	72-73	2.9436001806310905
1106	74-75	2.9011255795759645
1106	76-77	3.640687314476576
1106	78-79	4.304791941718346
1106	80-81	3.746446301094391
1106	82-83	2.315194434155199
1106	84-85	2.930049903154174
1106	86-87	3.4443893361344387
1106	88-89	3.5038955583001297
1106	90-91	3.356953887853706
1106	92-93	3.0965066243199324
1106	94-95	1.5057059625476477
1106	96-97	1.6739582064538006
1106	98-99	2.1192031255428603
1106	100	2.877585575892823
1107	1	6.2115507677582755
1107	2	10.51474063998797
1107	3	6.368167963111226
1107	4	11.677693590711069
1107	5	9.640200024199505
1107	6	8.206642586247717
1107	7	3.9566750679097424
1107	8	6.4754442252306745
1107	9	5.847973475362924
1107	10-11	4.75034463214136
1107	12-13	6.2813048967950245
1107	14-15	6.265720061114973
1107	16-17	7.088403872938429
1107	18-19	6.460271546106895
1107	20-21	5.623044534053488
1107	22-23	5.95961172786723
1107	24-25	4.788802433536343
1107	26-27	7.3110330839118305
1107	28-29	6.563450466781944
1107	30-31	5.1185639446963584
1107	32-33	5.05626317512084
1107	34-35	6.914430396580872
1107	36-37	6.63198142043586
1107	38-39	6.603359631629765
1107	40-41	7.734922210346301
1107	42-43	6.272711937639784
1107	44-45	4.3118589591833185
1107	46-47	3.7611624871912746
1107	48-49	4.240635884662321
1107	50-51	5.074163914854097
1107	52-53	4.053001282377927
1107	54-55	5.399428891704815
1107	56-57	5.47396341055094
1107	58-59	3.0632377608079047
1107	60-61	2.9141559550494804
1107	62-63	0.6101095804193299
1107	64-65	2.350777625372288
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2103	5	10.822746254568148
2103	6	9.512924480915181
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2103	9	6.7336936772332585
2103	10-11	7.59983309263276
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2103	14-15	6.435278711251524
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2103	78-79	3.649438531303776
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2104	2	11.02055941848333
2104	3	8.043839874842615
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2206	84-85	3.6787440984230706
2206	86-87	-1.6301921667887669
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2206	94-95	2.8050292262111434
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2206	98-99	1.433970946034954
2206	100	1.718176795638282
2207	1	6.4122664128508085
2207	2	10.850456883427523
2207	3	7.803100344383459
2207	4	12.20079557606508
2207	5	10.472777841021493
2207	6	9.335078830672565
2207	7	5.942484960470146
2207	8	6.975127128884676
2207	9	6.144599092299142
2207	10-11	6.962287463990034
2207	12-13	8.80481400628371
2207	14-15	8.322256823059437
2207	16-17	7.523336575902611
2207	18-19	4.314503229013489
2207	20-21	7.6606553776088155
2207	22-23	6.451443693642151
2207	24-25	4.424541360853635
2207	26-27	4.0546669841398995
2207	28-29	5.2380837425599935
2207	30-31	5.666475226469647
2207	32-33	7.01519703838191
2207	34-35	6.5547606492083155
2207	36-37	6.982288415654775
2207	38-39	7.0341008723717025
2207	40-41	4.247563417025656
2207	42-43	5.846281368963794
2207	44-45	4.154402884380197
2207	46-47	5.7371387625022265
2207	48-49	2.5842605109711734
2207	50-51	5.559475001075441
2207	52-53	2.974042867587599
2207	54-55	3.3065838628472086
2207	56-57	5.310251714181078
2207	58-59	3.8723909812108204
2207	60-61	3.6660745717667638
2207	62-63	4.436657097904206
2207	64-65	3.5601128682690053
2207	66-67	3.5490800518029317
2207	68-69	3.374609971478236
2207	70-71	3.5889511983069795
2207	72-73	3.8945477981331678
2207	74-75	3.5803526474574205
2207	76-77	4.116861472173024
2207	78-79	4.280194834812086
2207	80-81	4.307713961427883
2207	82-83	3.8152059773101072
2207	84-85	3.2108790962090517
2207	86-87	3.3900847102729017
2207	88-89	3.2985023620729663
2207	90-91	1.3677805349417014
2207	92-93	-0.7107822692540289
2207	94-95	1.2990807772638284
2207	96-97	-0.9929269479393632
2207	98-99	0.8156132814232251
2207	100	2.1996389505780805
2208	1	1.1718866090367257
2208	2	8.636437746164233
2208	3	6.62232362285426
2208	4	11.83755659202468
2208	5	10.145522170927588
2208	6	9.184281622971902
2208	7	7.585071358187104
2208	8	7.786985012198176
2208	9	6.4361305041811185
2208	10-11	6.752159317713723
2208	12-13	7.086761291903127
2208	14-15	7.8411351940299845
2208	16-17	7.243379078394675
2208	18-19	6.560717521200672
2208	20-21	6.672196505195021
2208	22-23	5.854653411723309
2208	24-25	6.206335408722044
2208	26-27	8.01067660773807
2208	28-29	7.156453717765636
2208	30-31	6.115558087759407
2208	32-33	8.487746950212635
2208	34-35	6.7003568205671975
2208	36-37	6.915136082623018
2208	38-39	7.258107382190651
2208	40-41	7.63552732333299
2208	42-43	7.081679319158184
2208	44-45	6.00820857326671
2208	46-47	4.708051533602315
2208	48-49	4.777441958432831
2208	50-51	6.086972820984315
2208	52-53	5.706449034534295
2208	54-55	6.3216211138080105
2208	56-57	6.520580161436243
2208	58-59	5.8911055668003804
2208	60-61	5.203449756907382
2208	62-63	3.489071970359589
2208	64-65	3.4370009608861096
2208	66-67	3.0606121321966704
2208	68-69	5.604549344889591
2208	70-71	5.473338774684446
2208	72-73	4.826201428820529
2208	74-75	2.1836222641922056
2208	76-77	3.507233773799591
2208	78-79	4.277672599368184
2208	80-81	3.565222418101637
2208	82-83	1.3080894616528198
2208	84-85	1.9327745944508656
2208	86-87	1.0493866800056608
2208	88-89	0.766604427477187
2208	90-91	1.994053165921727
2208	92-93	0.9404133120318043
2208	94-95	-1.149783510392325
2208	96-97	1.339406366081409
2208	98-99	0.27969987895092174
2208	100	1.831117921426511
2209	1	5.269683549902183
2209	2	9.675700560315118
2209	3	5.560374898571883
2209	4	-3.6455596495007967
2209	5	0.4415814363294892
2209	6	4.248304636320881
2209	7	2.2708141973921343
2209	8	5.2340409591022095
2209	9	5.055247698469831
2209	10-11	5.145351515641462
2209	12-13	7.241972156932746
2209	14-15	6.59171842333863
2209	16-17	6.133826377207981
2209	18-19	5.265205506532823
2209	20-21	5.848234642190025
2209	22-23	5.341072889262069
2209	24-25	5.074046145099221
2209	26-27	6.7918888666250155
2209	28-29	6.213684926054675
2209	30-31	3.953279072222596
2209	32-33	6.230075691756493
2209	34-35	5.3687958473960045
2209	36-37	6.0944233627927495
2209	38-39	6.81318316950685
2209	40-41	7.031701037948057
2209	42-43	6.72593661228138
2209	44-45	6.4037380846667915
2209	46-47	6.645763887324488
2209	48-49	5.009500559252974
2209	50-51	4.486934813376912
2209	52-53	4.743427804675754
2209	54-55	5.438955846879917
2209	56-57	5.049286476580328
2209	58-59	3.162585261033957
2209	60-61	4.066334159379274
2209	62-63	4.167200922746421
2209	64-65	4.982418993106563
2209	66-67	4.338467856201159
2209	68-69	3.837140169838463
2209	70-71	3.6369600542395872
2209	72-73	0.8128761528059023
2209	74-75	2.323325069697642
2209	76-77	3.395449371768791
2209	78-79	3.5270477307774684
2209	80-81	3.6171401187663683
2209	82-83	2.846447650638112
2209	84-85	2.8360147687124346
2209	86-87	1.5601901034839187
2209	88-89	1.9826486809769008
2209	90-91	2.222810618573952
2209	92-93	2.254031371600089
2209	94-95	0.7573181926719563
2209	96-97	0.13241105491158223
2209	98-99	0.16665803529524936
2209	100	0.15183367010286375
2210	1	1.835763026043928
2210	2	-17.27000002207611
2210	3	-7.786286841678292
2210	4	-8.141178519518293
2210	5	-1.5422505124394625
2210	6	2.4281243191602506
2210	7	-8.756795981203359
2210	8	-8.833032229041855
2210	9	-3.8044721313974605
2210	10-11	-1.0940037216376197
2210	12-13	3.4512122553468494
2210	14-15	-5.40357914262658
2210	16-17	-1.5741459381368372
2210	18-19	-0.28970975919567366
2210	20-21	2.432985886252972
2210	22-23	2.6123465014943186
2210	24-25	2.0042286076644906
2210	26-27	4.1769360549335985
2210	28-29	3.4478603790698976
2210	30-31	2.61148511599972
2210	32-33	4.572349992010526
2210	34-35	2.3609590637748497
2210	36-37	3.9718661325810665
2210	38-39	3.080974736583535
2210	40-41	4.17487455659866
2210	42-43	2.2270946149308486
2210	44-45	3.4882008874127237
2210	46-47	2.9654904131755977
2210	48-49	3.0271558392466993
2210	50-51	2.2383699499831806
2210	52-53	2.2724899772862273
2210	54-55	2.7072011592076315
2210	56-57	2.806901810963609
2210	58-59	1.7112051366926053
2210	60-61	2.7613682983954106
2210	62-63	1.83109254213635
2210	64-65	2.7053858813790654
2210	66-67	2.718257168069762
2210	68-69	3.3118179008607314
2210	70-71	2.5098252315403755
2210	72-73	1.026916605612481
2210	74-75	0.32041534738522515
2210	76-77	-0.2330811154075576
2210	78-79	0.2974287754596432
2210	80-81	0.7791302132424711
2210	82-83	0.775153892420704
2210	84-85	-1.2302569325529085
2210	86-87	-0.9523349051267118
2210	88-89	-1.420179388837827
2210	90-91	-1.7597068207838227
2210	92-93	-1.1190547022366495
2210	94-95	-0.4041202361954426
2210	96-97	-0.09833025378156535
2210	98-99	-0.33820765307058664
2210	100	0.04223822119789844
2211	1	1.1574818753349945
2211	2	-17.27000002207611
2211	3	-8.104671754734483
2211	4	-19.34956787149463
2211	5	-21.36263233551018
2211	6	-13.633360506532743
2211	7	-11.383117086335696
2211	8	-19.059151114783155
2211	9	-16.19469161287222
2211	10-11	-10.034844324019105
2211	12-13	-22.634697935564684
2211	14-15	-18.083690873284226
2211	16-17	-15.987377173430328
2211	18-19	-19.39360076955396
2211	20-21	-8.533506911202139
2211	22-23	-4.182811011136412
2211	24-25	-2.003771501755235
2211	26-27	-17.664456858188863
2211	28-29	-3.828689610039909
2211	30-31	-4.7646094264411545
2211	32-33	-1.0362349347835327
2211	34-35	-6.0608169633640046
2211	36-37	-6.994358206853423
2211	38-39	-2.7443216976795455
2211	40-41	-5.721378801499657
2211	42-43	-2.009230508011015
2211	44-45	-0.06983497326317689
2211	46-47	0.7902444517817777
2211	48-49	0.6389576024575803
2211	50-51	-5.041068659509548
2211	52-53	-1.097194709233161
2211	54-55	-0.542836896075908
2211	56-57	-7.060538390465336
2211	58-59	-2.41484423127903
2211	60-61	-1.1220008868694435
2211	62-63	-0.43519425628731767
2211	64-65	-3.7870814161021045
2211	66-67	-2.836586708619386
2211	68-69	-0.0566638590088786
2211	70-71	-0.4563361138873461
2211	72-73	-1.3236052177550839
2211	74-75	-2.004543206010048
2211	76-77	-1.9386240443561817
2211	78-79	-1.2241570270374034
2211	80-81	-1.096642110539129
2211	82-83	-1.671648014036819
2211	84-85	-1.9006685785397082
2211	86-87	-2.1807080270855295
2211	88-89	-2.7376551603073054
2211	90-91	-2.591932250618683
2211	92-93	-2.439293062928943
2211	94-95	-2.5399963671967463
2211	96-97	-3.2198969941195763
2211	98-99	-3.0678904894646237
2211	100	-2.532837518308593
2212	1	-21.294379485383875
2212	2	-17.27000002207611
2212	3	-20.197418866416125
2212	4	-19.34956787149463
2212	5	-21.36263233551018
2212	6	-22.141383613079597
2212	7	-22.694413620353668
2212	8	-23.065890524282512
2212	9	-24.53262486062575
2212	10-11	-11.602956964959308
2212	12-13	-22.634697935564684
2212	14-15	-24.05079613644212
2212	16-17	-24.49267242375125
2212	18-19	-19.030691040798683
2212	20-21	-24.19621551197236
2212	22-23	-19.157069988905747
2212	24-25	-25.778643132050487
2212	26-27	-17.616411599757942
2212	28-29	-18.594344689448384
2212	30-31	-5.9532326034291465
2212	32-33	-17.758126835442994
2212	34-35	-14.007965951004365
2212	36-37	-18.150422864402906
2212	38-39	-24.277858283045397
2212	40-41	-10.659997691773686
2212	42-43	-14.5135943828297
2212	44-45	-17.704716544100545
2212	46-47	-17.60754754295367
2212	48-49	-18.387596389284123
2212	50-51	-9.326874190992566
2212	52-53	-2.3589964962135994
2212	54-55	-24.255288757436627
2212	56-57	-24.164999237705388
2212	58-59	-13.138005230987073
2212	60-61	-6.655755399504823
2212	62-63	-3.2535105740695336
2212	64-65	-17.980277358321835
2212	66-67	-18.392313295051125
2212	68-69	-15.140335302079
2212	70-71	-10.167976048451235
2212	72-73	-18.292446252498323
2212	74-75	-6.662937287100771
2212	76-77	-11.58595226298323
2212	78-79	-4.824574344434975
2212	80-81	-2.6899945757472317
2212	82-83	-3.193264406431034
2212	84-85	-5.427800233952784
2212	86-87	-5.024815764951811
2212	88-89	-3.531262974639297
2212	90-91	-4.2847237355827446
2212	92-93	-5.60670743292102
2212	94-95	-3.941855526630068
2212	96-97	-5.615439390879285
2212	98-99	-4.053153346130809
2212	100	-4.076303407651128
2213	1	-21.294379485383875
2213	2	-17.27000002207611
2213	3	-20.197418866416125
2213	4	-19.34956787149463
2213	5	-21.36263233551018
2213	6	-22.141383613079597
2213	7	-10.917827382641425
2213	8	-10.798687664586355
2213	9	-4.30429599556766
2213	10-11	-17.997698272792825
2213	12-13	-16.611686318049045
2213	14-15	-24.05079613644212
2213	16-17	-18.41134981427851
2213	18-19	-18.97182111116192
2213	20-21	-18.126733831900864
2213	22-23	-20.375481863871467
2213	24-25	-7.875157877358799
2213	26-27	-23.78031051672545
2213	28-29	-24.719254436740805
2213	30-31	-20.01372595263534
2213	32-33	-23.918776654937577
2213	34-35	-18.442518946039257
2213	36-37	-24.340314561153807
2213	38-39	-11.959046844260769
2213	40-41	-17.72023391351628
2213	42-43	-23.904930122901902
2213	44-45	-17.77031340566144
2213	46-47	-17.621733107037127
2213	48-49	-6.415083863595573
2213	50-51	-24.107018595324696
2213	52-53	-18.440607043278895
2213	54-55	-18.391124324907583
2213	56-57	-18.257715949054806
2213	58-59	-18.661164930693168
2213	60-61	-20.146576400721095
2213	62-63	-18.269543132794283
2213	64-65	-18.268290282445037
2213	66-67	-9.092481218319385
2213	68-69	-23.52120172807178
2213	70-71	-18.177030193623402
2213	72-73	-9.92844377478324
2213	74-75	-4.7491411243329615
2213	76-77	-3.29974858706062
2213	78-79	-4.883122945397027
2213	80-81	-3.753664271807729
2213	82-83	-3.7283348825846403
2213	84-85	-3.4341080835993587
2213	86-87	-3.4447865356567
2213	88-89	-2.947353927430438
2213	90-91	-2.9982927744680303
2213	92-93	-4.031756390377232
2213	94-95	-3.857850338914112
2213	96-97	-3.486971799586133
2213	98-99	-4.149783105166566
2213	100	-3.6885887449333197
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	665.0
3	584.0
4	3114.0
5	11783.0
6	18331.0
7	21222.0
8	19129.0
9	18905.0
10	22284.0
11	27165.0
12	34604.0
13	47436.0
14	35232.0
15	39052.0
16	45999.0
17	49007.0
18	49993.0
19	49923.0
20	49492.0
21	48158.0
22	50033.0
23	55538.0
24	61844.0
25	68515.0
26	75875.0
27	82487.0
28	89277.0
29	94085.0
30	98813.0
31	107719.0
32	122550.0
33	150626.0
34	183571.0
35	219539.0
36	247699.0
37	222905.0
38	74602.0
39	191.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.16621941245754	21.509448583681117	14.504118613399461	23.820213390461884
2	14.346537012138349	21.573266677468954	41.22828092785207	22.85191538254063
3	17.078723657510103	27.594572407104646	30.18524873740524	25.14145519798001
4	11.75943668826231	18.264490880149243	40.72031105838935	29.255761373199096
5	12.978173592002392	37.68460958860251	36.163129220791184	13.17408759860391
6	29.031221182755505	38.96244721894166	19.561454224059904	12.444877374242928
7	24.93045413813008	32.809452477376986	25.222369371689396	17.037724012803533
8	23.10815023709307	36.638169001183996	22.65547599700514	17.598204764717796
9	24.05376899573934	17.550202481889446	22.88424788701758	35.51178063535364
10-11	22.777381505473198	27.713518425837314	29.69355331292239	19.8155467557671
12-13	23.546170633603865	25.380964889837017	29.864241613608318	21.2086228629508
14-15	21.1098363500066	26.64137714047102	27.06949209809536	25.179294411427023
16-17	20.78499847023545	30.266579188032654	27.816309040783437	21.132113300948454
18-19	20.706609812056296	29.45114833881584	29.2809347837509	20.561307065376962
20-21	22.150882791565426	28.793743077529786	28.192667765433395	20.862706365471393
22-23	21.1571891415617	28.798500483895808	28.834152743826884	21.210157630715603
24-25	21.453799558096758	28.547407994158284	28.36388034638687	21.63491210135808
26-27	21.41714552642808	28.65792204289296	28.36369290748672	21.561239523192242
28-29	21.182505470477476	28.521563448510207	28.7987602986971	21.497170782315216
30-31	21.78742632597166	28.898797208781247	28.096118745423333	21.217657719823755
32-33	21.01341648856563	28.752530424584872	28.744422957902877	21.489630128946622
34-35	21.21635782996343	28.151740140268068	28.531326541831636	22.10057548793687
36-37	21.689015312149447	28.777611344365884	28.126888392344174	21.406484951140495
38-39	21.308110029406645	28.454912775008584	28.51980413566364	21.717173059921127
40-41	21.682706009646306	28.492518943294957	28.08080230623529	21.74397274082344
42-43	21.26260804512916	28.681520436165325	28.574642932442785	21.48122858626273
44-45	21.705540082426385	28.141433930240012	28.485383330303453	21.667642657030147
46-47	21.570753533939133	28.64443217197864	28.431983262244714	21.352831031837518
48-49	21.31123080347919	28.33335566814523	28.644596272273226	21.710817256102356
50-51	21.76690609380689	28.396715358705883	28.211270174102598	21.625108373384627
52-53	21.165426041632912	28.51133650770712	28.491108378692314	21.832129071967657
54-55	21.619740596093056	28.39232385596863	28.237708910032328	21.750226637905985
56-57	21.716053852273127	28.46659781157032	28.199673321717565	21.617675014438987
58-59	21.370099215483684	27.99660728146464	28.552888705632817	22.080404797418854
60-61	21.820247185394663	28.398337815225165	28.082325325570697	21.69908967380948
62-63	21.368795870256648	28.535778899503168	28.504294929908937	21.591130300331248
64-65	21.425038693367437	28.02482331437365	28.465923678892135	22.08421431336678
66-67	21.53905798904413	28.308514488149854	28.21208376451361	21.940343758292403
68-69	21.842293781913572	28.185724924776967	28.224460837425053	21.747520455884413
70-71	21.99006505936408	28.278880509508408	27.995447718287135	21.735606712840376
72-73	21.561249291800756	28.465866995149945	28.45937862955165	21.513505083497645
74-75	21.820606991872758	27.99744677713818	28.106521389061882	22.07542484192718
76-77	21.834031827034245	28.412148051483683	28.12222598980186	21.631594131680217
78-79	21.751677132264373	28.143301417045585	28.32440664055097	21.780614810139074
80-81	22.020116776546157	28.120309348036578	28.027942608470514	21.83163126694675
82-83	21.41148847441668	28.36992204026423	28.595970163122054	21.62261932219704
84-85	21.708854592930354	27.867804668927082	28.185568416540008	22.237772321602556
86-87	21.842513665660597	28.245909775415868	28.198889810684708	21.71268674823883
88-89	21.574579016623773	27.89438913697342	28.452017161125536	22.079014685277272
90-91	21.89353705257654	27.943752975872123	28.113973826613382	22.04873614493796
92-93	21.51052444967661	28.22017493242862	28.457341005455923	21.81195961243885
94-95	21.547191701727826	27.721477398322637	28.35244282631646	22.378888073633078
96-97	21.71597297627325	28.038596919685283	28.31861849315108	21.926811610890383
98-99	21.580359228271746	27.851676032526058	28.382246940047672	22.18571779915452
100	21.784903642699156	27.59587886820041	28.143476440430206	22.47574104867023
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	255.0
1	301.5
2	294.5
3	250.5
4	283.0
5	384.0
6	590.5
7	870.0
8	1302.0
9	1938.5
10	2686.5
11	3535.5
12	4504.5
13	5384.0
14	6120.0
15	6834.5
16	7619.0
17	8354.0
18	9072.0
19	9729.5
20	10401.5
21	11392.5
22	12573.0
23	13888.5
24	15681.0
25	18136.0
26	21308.0
27	25530.0
28	31554.5
29	39056.0
30	47353.0
31	56312.0
32	65545.0
33	74398.5
34	82223.5
35	89460.5
36	95406.0
37	100117.0
38	103689.5
39	105965.5
40	107210.5
41	105909.0
42	102645.0
43	98891.5
44	94214.5
45	89069.5
46	84203.5
47	79224.5
48	75173.5
49	72146.0
50	69165.0
51	64545.0
52	58119.0
53	52428.0
54	47938.0
55	44023.0
56	40346.5
57	36342.0
58	32375.0
59	28613.0
60	24569.5
61	20677.0
62	17089.5
63	13862.5
64	11174.5
65	8884.5
66	7013.0
67	5480.5
68	4185.0
69	3162.5
70	2341.5
71	1777.5
72	1375.5
73	1019.5
74	766.0
75	571.5
76	378.5
77	248.5
78	175.5
79	134.0
80	87.0
81	62.0
82	47.5
83	31.5
84	20.0
85	10.0
86	9.0
87	8.0
88	4.5
89	3.5
90	4.5
91	5.0
92	4.0
93	2.5
94	1.0
95	1.0
96	0.5
97	0.5
98	0.5
99	0.5
100	1.5
>>END_MODULE
>>Per base N content	fail
#Base	N-Count
1	7.611625641323706
2	24.220201566852595
3	5.896194187179338
4	24.070044539014845
5	12.235238055279805
6	9.58807088828217
7	7.777140950142555
8	8.386198794663633
9	5.149296733151215
10-11	6.669400876923201
12-13	9.809688188404152
14-15	7.26654546840255
16-17	7.6554679521945594
18-19	6.593263834866531
20-21	7.714187394892967
22-23	5.638663914236895
24-25	5.168908372649635
26-27	9.522827063061717
28-29	6.206092733993419
30-31	4.27830128944124
32-33	8.606507369087977
34-35	7.052241635414426
36-37	6.87315792046566
38-39	5.892787651172252
40-41	8.429925629737635
42-43	6.252956661548523
44-45	6.201281242457987
46-47	5.7672077220974876
48-49	3.7765397061602877
50-51	6.388178819660293
52-53	4.379708285041996
54-55	4.762837733025346
56-57	4.629809615053733
58-59	3.4728768523761264
60-61	4.054124275822409
62-63	3.1098786849770224
64-65	4.350550646337281
66-67	2.303953852792224
68-69	5.002142076031575
70-71	2.423240350938645
72-73	1.5212589017404898
74-75	0.8010940946832249
76-77	1.0690556812744834
78-79	1.7006890440798061
80-81	2.0269274161482125
82-83	0.785177680684017
84-85	1.2335509538878198
86-87	0.4546474581660057
88-89	0.7170662065084469
90-91	0.322177473212502
92-93	0.3399992378597408
94-95	0.5346914313494463
96-97	0.5472590472399937
98-99	0.460325018177815
100	0.4378842216565619
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2597947.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.66208466902987
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.98282798852831	92.77579502480556
2	2.4676728091516864	4.721254504090628
3	0.3243890218207134	0.9309519021335058
4	0.10114583496648798	0.38703285713935576
5	0.04562557698751004	0.2182318904426263
6	0.02558112833898146	0.14682864390557784
7	0.01234033918270379	0.08263518002182497
8	0.00802912042410453	0.061446591826281954
9	0.006732960320951153	0.0579680118266443
>10	0.02406456745589782	0.3531814509140893
>50	8.615679700048126E-4	0.060079704975645354
>100	6.48368474294483E-4	0.13077672641276597
>500	4.035818917246888E-5	0.03239167999642866
>1k	4.035818917246872E-5	0.04142583150913924
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	7.69838645669061E-5	0.0
8	0.0	0.0	0.0	7.69838645669061E-5	0.0
9	0.0	0.0	0.0	7.69838645669061E-5	0.0
10-11	0.0	0.0	0.0	1.1547579685035915E-4	0.0
12-13	0.0	0.0	0.0	1.1547579685035915E-4	0.0
14-15	0.0	0.0	0.0	1.539677291338122E-4	0.0
16-17	0.0	0.0	0.0	1.7321369527553872E-4	0.0
18-19	0.0	0.0	0.0	1.9245966141726524E-4	0.0
20-21	0.0	0.0	0.0	2.309515937007183E-4	0.0
22-23	0.0	3.849193228345305E-5	0.0	2.309515937007183E-4	0.0
24-25	0.0	3.849193228345305E-5	0.0	2.501975598424448E-4	0.0
26-27	0.0	3.849193228345305E-5	0.0	3.079354582676244E-4	0.0
28-29	0.0	3.849193228345305E-5	0.0	5.581330181100692E-4	0.0
30-31	3.849193228345305E-5	3.849193228345305E-5	0.0	0.0014434474606294895	0.0
32-33	3.849193228345305E-5	3.849193228345305E-5	0.0	0.004118636754329476	0.0
34-35	3.849193228345305E-5	7.69838645669061E-5	0.0	0.007659894524407157	0.0
36-37	3.849193228345305E-5	7.69838645669061E-5	0.0	0.013914833520468278	0.0
38-39	3.849193228345305E-5	7.69838645669061E-5	0.0	0.025597134968496277	0.0
40-41	3.849193228345305E-5	7.69838645669061E-5	0.0	0.0407052183897516	3.849193228345305E-5
42-43	3.849193228345305E-5	7.69838645669061E-5	0.0	0.056602386422817716	3.849193228345305E-5
44-45	3.849193228345305E-5	7.69838645669061E-5	0.0	0.07502077602044999	3.849193228345305E-5
46-47	3.849193228345305E-5	7.69838645669061E-5	0.0	0.09686494759130959	3.849193228345305E-5
48-49	3.849193228345305E-5	7.69838645669061E-5	0.0	0.12361684052830947	3.849193228345305E-5
50-51	3.849193228345305E-5	7.69838645669061E-5	0.0	0.15298618486058413	3.849193228345305E-5
52-53	3.849193228345305E-5	7.69838645669061E-5	0.0	0.18270195658340987	3.849193228345305E-5
54-55	3.849193228345305E-5	7.69838645669061E-5	0.0	0.21534311515977808	3.849193228345305E-5
56-57	3.849193228345305E-5	9.622983070863262E-5	0.0	0.254335442562916	3.849193228345305E-5
58-59	3.849193228345305E-5	1.1547579685035915E-4	0.0	0.30044877743849274	3.849193228345305E-5
60-61	3.849193228345305E-5	1.1547579685035915E-4	0.0	0.3532789544975321	3.849193228345305E-5
62-63	3.849193228345305E-5	1.1547579685035915E-4	0.0	0.4084186474935786	3.849193228345305E-5
64-65	3.849193228345305E-5	1.1547579685035915E-4	0.0	0.4711027592171819	3.849193228345305E-5
66-67	3.849193228345305E-5	1.1547579685035915E-4	0.0	0.546046551373065	3.849193228345305E-5
68-69	3.849193228345305E-5	1.1547579685035915E-4	0.0	0.6305170967691027	3.849193228345305E-5
70-71	3.849193228345305E-5	1.1547579685035915E-4	0.0	0.7274205362926958	3.849193228345305E-5
72-73	3.849193228345305E-5	1.1547579685035915E-4	0.0	0.8347552894651046	3.849193228345305E-5
74-75	3.849193228345305E-5	1.1547579685035915E-4	0.0	0.9470747478682205	3.849193228345305E-5
76-77	3.849193228345305E-5	1.7321369527553872E-4	0.0	1.0695175844618847	3.849193228345305E-5
78-79	3.849193228345305E-5	1.9245966141726524E-4	0.0	1.2134004273374321	3.849193228345305E-5
80-81	3.849193228345305E-5	2.309515937007183E-4	0.0	1.3774915346617926	3.849193228345305E-5
82-83	5.773789842517957E-5	2.309515937007183E-4	0.0	1.5597315880578009	3.849193228345305E-5
84-85	7.69838645669061E-5	2.309515937007183E-4	0.0	1.747495233736485	3.849193228345305E-5
86-87	7.69838645669061E-5	2.309515937007183E-4	0.0	1.9517719183647704	3.849193228345305E-5
88	7.69838645669061E-5	2.309515937007183E-4	0.0	2.121328880073381	3.849193228345305E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATATA	1615	0.0	19.168535	1
GTACATG	6730	0.0	17.847528	1
CGGTACT	190	0.0012221995	17.744879	4
TACATGG	6865	0.0	17.131449	2
CTGTCGC	545	0.0	16.578157	9
GTATAAG	1020	0.0	16.3891	1
TAATACG	245	3.6028665E-4	16.05489	4
GTATTAA	1415	0.0	15.752067	1
CCTATAC	680	0.0	15.699933	3
ACATGGG	7245	0.0	15.4336195	3
TACTGCG	290	6.222285E-4	14.941049	2
GTATAAA	1705	0.0	14.525366	1
GTGTATA	1250	0.0	14.364135	1
TCTGTCG	455	2.048182E-8	14.336496	8
GCACCGT	245	8.560259E-4	14.325341	6
ATACCGT	245	8.560259E-4	14.325341	6
GTATCAA	4380	0.0	14.277059	1
CGCAGCG	320	2.8151004E-5	14.112488	8
TATACAG	1410	0.0	13.830806	5
TCAACGC	4515	0.0	13.814673	4
>>END_MODULE