FastQCFastQC Report
Wed 25 May 2016
SRR1295139_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295139_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2597947
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT27530.10596828957634624No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA70300.022.2379681
CAACGCA76650.019.6898045
AACGCAG77700.019.459856
TCAACGC77650.019.4362344
ATCAACG77500.019.4128113
TATCAAC79350.019.1282752
ACGCAGA85500.017.738467
CGCAGAG86050.017.5705178
GAGTACT29150.016.42918612-13
GTACATG91800.016.3269161
CATGGGG39200.016.1918414
TACATGG92750.016.150812
TAATACG2951.3705394E-615.9377334
GCAGAGT97400.015.3778129
GTACTAG5557.2759576E-1215.2018291
ACATGGG94400.015.1405543
GTATATA19850.014.7513361
TATACTG15600.014.1651725
AGTACTT38900.014.06147212-13
ACCGATC2050.006713699513.7428548
Produced by FastQC (version 0.11.5)