##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295139_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2597947 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.407382829595832 33.0 33.0 33.0 27.0 33.0 2 30.14482704997446 33.0 33.0 33.0 27.0 33.0 3 30.964224058458466 33.0 33.0 33.0 27.0 33.0 4 35.20922905663588 37.0 37.0 37.0 33.0 37.0 5 35.4634382456609 37.0 37.0 37.0 33.0 37.0 6 35.25429425619537 37.0 37.0 37.0 33.0 37.0 7 35.163018722090946 37.0 37.0 37.0 33.0 37.0 8 35.169458037442645 37.0 37.0 37.0 33.0 37.0 9 35.13865371387484 37.0 37.0 37.0 33.0 37.0 10-11 35.1093919544933 37.0 37.0 37.0 33.0 37.0 12-13 35.018790414123146 37.0 37.0 37.0 33.0 37.0 14-15 36.49824880954077 40.0 37.0 40.0 33.0 40.0 16-17 36.4896279639269 40.0 37.0 40.0 33.0 40.0 18-19 36.51756271394297 40.0 37.0 40.0 33.0 40.0 20-21 36.52004967768781 40.0 37.0 40.0 33.0 40.0 22-23 36.47567252141788 40.0 37.0 40.0 33.0 40.0 24-25 36.44559742750718 40.0 37.0 40.0 33.0 40.0 26-27 36.431679899551455 40.0 37.0 40.0 33.0 40.0 28-29 36.383050539522166 40.0 37.0 40.0 33.0 40.0 30-31 36.293301018073116 37.0 37.0 40.0 33.0 40.0 32-33 36.254725750756265 37.0 37.0 40.0 30.0 40.0 34-35 36.154553191423844 37.0 37.0 40.0 27.0 40.0 36-37 36.15015799013606 37.0 37.0 40.0 27.0 40.0 38-39 36.085791011133026 37.0 37.0 40.0 27.0 40.0 40-41 36.0350328547888 37.0 37.0 40.0 27.0 40.0 42-43 35.945688653386696 37.0 37.0 40.0 27.0 40.0 44-45 35.854238365909694 37.0 37.0 40.0 27.0 40.0 46-47 35.75754528479603 37.0 37.0 40.0 27.0 40.0 48-49 35.65728072974545 37.0 37.0 40.0 27.0 40.0 50-51 35.56331095284084 37.0 37.0 40.0 27.0 40.0 52-53 35.470337732063044 37.0 37.0 40.0 27.0 40.0 54-55 35.35400144806649 37.0 33.0 40.0 27.0 40.0 56-57 35.23393279385607 37.0 33.0 40.0 27.0 40.0 58-59 35.11285815299543 37.0 33.0 40.0 27.0 40.0 60-61 34.95436069327049 37.0 33.0 40.0 27.0 40.0 62-63 34.77354349415134 37.0 33.0 40.0 27.0 40.0 64-65 34.59576446324733 37.0 33.0 40.0 27.0 40.0 66-67 34.3743369283515 37.0 33.0 40.0 27.0 40.0 68-69 34.1648301524242 37.0 33.0 38.5 27.0 40.0 70-71 33.936578382853845 37.0 33.0 37.0 27.0 40.0 72-73 33.72144389396705 37.0 33.0 37.0 27.0 40.0 74-75 33.48491481927845 37.0 33.0 37.0 24.5 40.0 76-77 33.24791441087905 37.0 33.0 37.0 22.0 38.5 78-79 33.013056270970885 37.0 33.0 37.0 22.0 37.0 80-81 32.796076478850416 37.0 33.0 37.0 22.0 37.0 82-83 32.5832216746531 37.0 33.0 37.0 22.0 37.0 84-85 32.412220303185556 35.0 33.0 37.0 22.0 37.0 86-87 32.43444169569279 35.0 33.0 37.0 22.0 37.0 88-89 32.449037643955016 35.0 33.0 37.0 22.0 37.0 90-91 32.45680185161591 33.0 33.0 37.0 22.0 37.0 92-93 32.42443090640417 33.0 33.0 37.0 22.0 37.0 94-95 32.450388518318505 33.0 33.0 37.0 22.0 37.0 96-97 32.40789457983554 33.0 33.0 37.0 22.0 37.0 98-99 32.3372803602229 33.0 33.0 37.0 22.0 37.0 100 31.808278228924607 33.0 33.0 37.0 22.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5861926190568809 1101 2 4.022109723483116 1101 3 2.2759566599273846 1101 4 1.0213274166130262 1101 5 0.5273457829063801 1101 6 0.46757516491325646 1101 7 0.5092033735580088 1101 8 0.49200825387772085 1101 9 0.5824992338474644 1101 10-11 0.6080160682829501 1101 12-13 0.650627926207747 1101 14-15 0.9828777330109801 1101 16-17 0.969042680262163 1101 18-19 0.9263223478819782 1101 20-21 0.9311723960654135 1101 22-23 0.908606912258854 1101 24-25 0.9236430972875311 1101 26-27 0.8784364256644679 1101 28-29 0.81212103031541 1101 30-31 0.6997477971547141 1101 32-33 0.9857357671903983 1101 34-35 1.037262623715968 1101 36-37 0.9454402761133522 1101 38-39 0.9224989660486429 1101 40-41 0.8565955369541314 1101 42-43 0.9715257425665556 1101 44-45 0.970248628257643 1101 46-47 1.0137048831415 1101 48-49 0.933724087237529 1101 50-51 0.9535811157222511 1101 52-53 0.8848815095471707 1101 54-55 0.9609862728014136 1101 56-57 0.833962298638788 1101 58-59 0.8454709256219175 1101 60-61 0.9049829233462177 1101 62-63 0.8975462291918035 1101 64-65 0.8007702938407775 1101 66-67 0.8469655993586329 1101 68-69 0.8505822444183835 1101 70-71 0.8545968607794521 1101 72-73 0.7504043191729082 1101 74-75 0.7125366686313441 1101 76-77 0.8691642547067389 1101 78-79 0.8617942408858283 1101 80-81 0.7901898655319144 1101 82-83 0.8117513093086686 1101 84-85 0.8713041819305545 1101 86-87 0.9036940293403575 1101 88-89 0.7723026815747147 1101 90-91 0.872220962291415 1101 92-93 0.8008111654011714 1101 94-95 0.8773216524226441 1101 96-97 0.8171562444540896 1101 98-99 0.8693438172990113 1101 100 0.9575019304475525 1102 1 0.47914517416148783 1102 2 4.050296635164091 1102 3 2.3174838294669122 1102 4 1.0671305324311646 1102 5 0.4598119295998089 1102 6 0.5319044821986054 1102 7 0.46885628277041036 1102 8 0.5381046748780491 1102 9 0.5656202048562591 1102 10-11 0.5785532369761341 1102 12-13 0.6213389457884944 1102 14-15 0.8447494153448503 1102 16-17 0.8596300278640285 1102 18-19 0.8726935418154937 1102 20-21 0.8105521128072368 1102 22-23 0.9021023266572357 1102 24-25 0.9470751544622473 1102 26-27 0.8371586543723737 1102 28-29 0.8287469826649243 1102 30-31 0.8443222046020296 1102 32-33 0.9786252440681906 1102 34-35 1.0195311580301691 1102 36-37 0.9931221785539179 1102 38-39 0.9140355264039997 1102 40-41 0.8766513425591 1102 42-43 0.9922458100619735 1102 44-45 1.048188627724592 1102 46-47 1.0017588613950252 1102 48-49 0.893380331943014 1102 50-51 0.8395305069402141 1102 52-53 0.9496710937062645 1102 54-55 0.9788764237917036 1102 56-57 0.8798685186008441 1102 58-59 0.8732724631595374 1102 60-61 0.9424430513449877 1102 62-63 0.9217812050164866 1102 64-65 0.9226223559937949 1102 66-67 0.913909226252251 1102 68-69 0.881965587184105 1102 70-71 0.8587594782250534 1102 72-73 0.8680478774707012 1102 74-75 0.8178433757935935 1102 76-77 0.7808892298970633 1102 78-79 0.7164577200264546 1102 80-81 0.756907894635809 1102 82-83 0.7579651116102539 1102 84-85 0.6809385043415048 1102 86-87 0.7920016297601293 1102 88-89 0.725217536255542 1102 90-91 0.8037844528568812 1102 92-93 0.789108182644263 1102 94-95 0.7062434948056335 1102 96-97 0.715392111510063 1102 98-99 0.8097232654868805 1102 100 0.7969952908679936 1103 1 0.4910863619874348 1103 2 3.9578335599738246 1103 3 2.255338624815483 1103 4 1.02212225345081 1103 5 0.49398701204761153 1103 6 0.4890239303037376 1103 7 0.504702773045139 1103 8 0.40650302404733907 1103 9 0.48152670887186844 1103 10-11 0.5441196105597967 1103 12-13 0.5714115705343374 1103 14-15 0.7989153125071482 1103 16-17 0.745447565140978 1103 18-19 0.803936014606073 1103 20-21 0.7850153819252697 1103 22-23 0.7262833238386364 1103 24-25 0.734126869082651 1103 26-27 0.7705351401174809 1103 28-29 0.7432670329721418 1103 30-31 0.742933052878314 1103 32-33 0.8431543795936776 1103 34-35 0.9504284938603647 1103 36-37 0.8681486203707749 1103 38-39 0.8135159146192734 1103 40-41 0.8031601601291811 1103 42-43 0.8252533232113137 1103 44-45 0.9041783389128142 1103 46-47 0.855818984956521 1103 48-49 0.7614138948736624 1103 50-51 0.7198384879228783 1103 52-53 0.7501374703552841 1103 54-55 0.7429030792964397 1103 56-57 0.6794324059570584 1103 58-59 0.7007986826751349 1103 60-61 0.7844655615328406 1103 62-63 0.8294726957041547 1103 64-65 0.7727275912581959 1103 66-67 0.7038851524821794 1103 68-69 0.7613244177755902 1103 70-71 0.8102585360730359 1103 72-73 0.7873248897974676 1103 74-75 0.7946581457681532 1103 76-77 0.6532709200250579 1103 78-79 0.7655497872027297 1103 80-81 0.6934185273495359 1103 82-83 0.736573808440987 1103 84-85 0.7252049874417921 1103 86-87 0.7596421342915249 1103 88-89 0.6408574212567615 1103 90-91 0.7211209999951365 1103 92-93 0.7547662792489191 1103 94-95 0.692352420904335 1103 96-97 0.7279119792895798 1103 98-99 0.7597648567231232 1103 100 0.8658472802806294 1104 1 0.5129268373100047 1104 2 3.7070231937459646 1104 3 2.2793210752757993 1104 4 1.0481174818709107 1104 5 0.490846129959003 1104 6 0.4654006172369307 1104 7 0.46026266080765055 1104 8 0.41969696541745094 1104 9 0.44166434872653326 1104 10-11 0.5126253151022624 1104 12-13 0.6174344443307405 1104 14-15 0.8064403271571905 1104 16-17 0.7667892118256603 1104 18-19 0.7542100331048545 1104 20-21 0.702134350865137 1104 22-23 0.707010722535486 1104 24-25 0.7479039761373869 1104 26-27 0.7285978867520697 1104 28-29 0.7042153434627068 1104 30-31 0.6438029773389786 1104 32-33 0.8216394926950343 1104 34-35 0.875264799241485 1104 36-37 0.7700650515858385 1104 38-39 0.7182799227720267 1104 40-41 0.6902211588848957 1104 42-43 0.7926836823435792 1104 44-45 0.8476236360842719 1104 46-47 0.7487577343432221 1104 48-49 0.6713912911352438 1104 50-51 0.6958104884078793 1104 52-53 0.745762646271082 1104 54-55 0.7978965534290623 1104 56-57 0.6372697945180761 1104 58-59 0.7070486121806425 1104 60-61 0.677955417631793 1104 62-63 0.6704199148118093 1104 64-65 0.6254580608232843 1104 66-67 0.620182315860724 1104 68-69 0.6232603945498383 1104 70-71 0.7559762392138083 1104 72-73 0.6806404963295165 1104 74-75 0.7852655557902608 1104 76-77 0.6339833386423734 1104 78-79 0.6675816371268724 1104 80-81 0.6370663840350019 1104 82-83 0.6921327028720228 1104 84-85 0.7481545654233059 1104 86-87 0.7857613368885978 1104 88-89 0.6389278428189442 1104 90-91 0.6939177523267119 1104 92-93 0.8144036261028447 1104 94-95 0.7388456211338124 1104 96-97 0.7341088937328522 1104 98-99 0.7510872090653606 1104 100 0.6822212089566086 1105 1 0.41754605893893526 1105 2 3.730209408169344 1105 3 2.1618223581434783 1105 4 0.9606326653218744 1105 5 0.27005880758661505 1105 6 0.19575303150005396 1105 7 0.17577138263833803 1105 8 0.21696843902095253 1105 9 0.20160925989704737 1105 10-11 0.23370402811553248 1105 12-13 0.2919328146211555 1105 14-15 0.4142192367250104 1105 16-17 0.32580324846797737 1105 18-19 0.40995211447463475 1105 20-21 0.3141594884259291 1105 22-23 0.30433962166952 1105 24-25 0.429444738068554 1105 26-27 0.3675173738824995 1105 28-29 0.3639532873674156 1105 30-31 0.32745506926508483 1105 32-33 0.4205040563285962 1105 34-35 0.5350032618367777 1105 36-37 0.4061543684175106 1105 38-39 0.3677168176931218 1105 40-41 0.33109181408542554 1105 42-43 0.4341547056782673 1105 44-45 0.5032585430135086 1105 46-47 0.4427584002770857 1105 48-49 0.2760940576070965 1105 50-51 0.33601229210897543 1105 52-53 0.29611267001900643 1105 54-55 0.3196182774255689 1105 56-57 0.2969685772842112 1105 58-59 0.30858352457882887 1105 60-61 0.3558559766732543 1105 62-63 0.39959394512868585 1105 64-65 0.23473477047848945 1105 66-67 0.25631460296489905 1105 68-69 0.34998941473977396 1105 70-71 0.31919252861358416 1105 72-73 0.3607261204732879 1105 74-75 0.2964316834023464 1105 76-77 0.24677134036974024 1105 78-79 0.3307736874676124 1105 80-81 0.24774474151181636 1105 82-83 0.3966629982432863 1105 84-85 0.41877830089214996 1105 86-87 0.3785850841771037 1105 88-89 0.36532919644574946 1105 90-91 0.40569318251814224 1105 92-93 0.2603304251819196 1105 94-95 0.25924852571026946 1105 96-97 0.3812035116794732 1105 98-99 0.33128225098641906 1105 100 0.3384277129020461 1106 1 0.5585369484050737 1106 2 3.9091903880670706 1106 3 2.2314627188046714 1106 4 1.0721138180683738 1106 5 0.39367322216347134 1106 6 0.30960356792478194 1106 7 0.33346666605658015 1106 8 0.23667740179517693 1106 9 0.34696729587300723 1106 10-11 0.28249589140602893 1106 12-13 0.3672965309451186 1106 14-15 0.39812088024009995 1106 16-17 0.24856299605583132 1106 18-19 0.3688609967839511 1106 20-21 0.33774167532943977 1106 22-23 0.2979630019241526 1106 24-25 0.20722423052040284 1106 26-27 0.21282940240840276 1106 28-29 0.28047212756258944 1106 30-31 0.24691190543797603 1106 32-33 0.34232108431469754 1106 34-35 0.42723642464202527 1106 36-37 0.33310915166033084 1106 38-39 0.24890017165869693 1106 40-41 0.35981348874377517 1106 42-43 0.30717677433557355 1106 44-45 0.4016781518122272 1106 46-47 0.27368897795452085 1106 48-49 0.29769306353817626 1106 50-51 0.2261728417472142 1106 52-53 0.08566787198096648 1106 54-55 0.26086827161047665 1106 56-57 0.21366336228841476 1106 58-59 0.16742328342150614 1106 60-61 0.22080171659673908 1106 62-63 0.31502031692630794 1106 64-65 0.19448415500051652 1106 66-67 0.154617492723645 1106 68-69 0.2308228201268392 1106 70-71 0.21765655673908668 1106 72-73 0.21694185049746295 1106 74-75 0.1863927711737503 1106 76-77 0.200234467756637 1106 78-79 0.2438361297477485 1106 80-81 0.2541995803389554 1106 82-83 0.2924246340936847 1106 84-85 0.23899230290987106 1106 86-87 0.25998668478078457 1106 88-89 0.25018557475788583 1106 90-91 0.3028582701785396 1106 92-93 0.36855321417343134 1106 94-95 0.3330314818750253 1106 96-97 0.288894022707062 1106 98-99 0.29037378351415555 1106 100 0.4645027881413917 1107 1 0.4048310229439913 1107 2 3.56153979797374 1107 3 2.1006362003639865 1107 4 0.9433141518731887 1107 5 0.28910892682565503 1107 6 0.20449499544952232 1107 7 0.1014799804171389 1107 8 0.12005209029157271 1107 9 0.12379727563128284 1107 10-11 0.1488402762702421 1107 12-13 0.08088161137055039 1107 14-15 0.079266582983891 1107 16-17 0.12343061145580947 1107 18-19 0.08898211610198814 1107 20-21 0.16022895311932217 1107 22-23 0.10097159594791094 1107 24-25 0.040539404468241 1107 26-27 0.12211677736361537 1107 28-29 0.1039648552173702 1107 30-31 0.038691331327108 1107 32-33 0.171071789527808 1107 34-35 0.30436272395630937 1107 36-37 0.18327312996554923 1107 38-39 0.08435572553808157 1107 40-41 0.1345323453582381 1107 42-43 0.11344488347536696 1107 44-45 0.24056923443475142 1107 46-47 0.2146930595601546 1107 48-49 0.10035024507859447 1107 50-51 0.1014592895018609 1107 52-53 0.03291216158722676 1107 54-55 0.15468210039237107 1107 56-57 0.11467314707604714 1107 58-59 0.1967793172477741 1107 60-61 0.19774085759159732 1107 62-63 0.24750305959883434 1107 64-65 0.1452036970056767 1107 66-67 0.10555819460596183 1107 68-69 0.14940966053091387 1107 70-71 0.14663378137021255 1107 72-73 0.1302396122428604 1107 74-75 0.19438067815796956 1107 76-77 0.19228480267277348 1107 78-79 0.12347723597589777 1107 80-81 0.21759533498359218 1107 82-83 0.15427982024551312 1107 84-85 0.1310784836963279 1107 86-87 0.11247564767336371 1107 88-89 0.13679294913931273 1107 90-91 0.17022571257568586 1107 92-93 0.25040265615852775 1107 94-95 0.046994551801113005 1107 96-97 0.03784865214446853 1107 98-99 0.041552590880741036 1107 100 0.12113629974250983 1108 1 0.35674661464241453 1108 2 3.614199879652972 1108 3 2.0203083447720225 1108 4 0.8586784899631112 1108 5 0.18596993555513563 1108 6 -0.09411231361897165 1108 7 -0.05921377971148445 1108 8 -0.08220623746508693 1108 9 -0.07844135876315761 1108 10-11 -0.013038290040547906 1108 12-13 -0.04663059120290569 1108 14-15 -0.16899425668756862 1108 16-17 -0.16026292685026533 1108 18-19 -0.11767722699838146 1108 20-21 -0.07980046559048759 1108 22-23 -0.21741141101383477 1108 24-25 -0.14042227872000979 1108 26-27 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78-79 0.16382753937971017 2211 80-81 0.24686247440708797 2211 82-83 -0.1167750042306821 2211 84-85 0.17704737614233323 2211 86-87 0.2202093656386097 2211 88-89 0.1605797486251035 2211 90-91 0.27629757234115715 2211 92-93 0.33085710172934313 2211 94-95 0.42576653389161123 2211 96-97 0.24778825430064444 2211 98-99 0.3527718621980398 2211 100 0.11542738151349141 2212 1 0.5863685366919924 2212 2 4.126066921077221 2212 3 2.365482283469973 2212 4 1.1534219438660216 2212 5 0.7017655869290707 2212 6 0.6498821538424693 2212 7 0.49219441631295524 2212 8 0.42935752948307027 2212 9 0.3712588563206225 2212 10-11 0.32845067343328793 2212 12-13 0.3614743874473376 2212 14-15 0.6636236111279032 2212 16-17 0.7393065883546939 2212 18-19 0.4627964648925129 2212 20-21 0.6644230438915955 2212 22-23 0.5860461083532229 2212 24-25 0.6036337318882588 2212 26-27 0.5568444343464449 2212 28-29 0.623303506894004 2212 30-31 0.6482463274831005 2212 32-33 0.7282445551621848 2212 34-35 0.7738971548290294 2212 36-37 0.6819134439280177 2212 38-39 0.8377718440421731 2212 40-41 0.7095214332323323 2212 42-43 0.772728690136141 2212 44-45 0.9665556235148287 2212 46-47 0.8890934997518016 2212 48-49 0.6408854536999087 2212 50-51 0.6852937498357221 2212 52-53 0.5996399233775875 2212 54-55 0.6766514850890673 2212 56-57 0.8632263237988056 2212 58-59 0.7300192824056637 2212 60-61 0.6771714842703531 2212 62-63 0.6593629268128041 2212 64-65 0.7536654994524241 2212 66-67 0.6587622056669957 2212 68-69 0.7725605584566821 2212 70-71 0.8017804384252614 2212 72-73 0.9353829780856984 2212 74-75 0.6928231359457797 2212 76-77 0.6883294685763914 2212 78-79 0.6689638337124393 2212 80-81 0.6460731397514436 2212 82-83 0.5854513064610813 2212 84-85 0.8079845908441357 2212 86-87 0.7751716425556907 2212 88-89 0.8502791225331521 2212 90-91 0.8078651657104245 2212 92-93 0.654123068001013 2212 94-95 0.8732878863614602 2212 96-97 0.8902724861446742 2212 98-99 0.8083304557851108 2212 100 1.0743764182772786 2213 1 -0.4923924767192851 2213 2 -25.91075618361592 2213 3 -14.806114003828835 2213 4 -6.3512011885723645 2213 5 -2.0036895803005663 2213 6 -1.223818255561234 2213 7 -1.1421793637833773 2213 8 -0.9886691382159754 2213 9 -1.0464577743740051 2213 10-11 -0.931548100613945 2213 12-13 -1.0368949340523272 2213 14-15 -1.2459328520628645 2213 16-17 -1.3046675311369853 2213 18-19 -1.3939407908444608 2213 20-21 -1.3712007241065152 2213 22-23 -1.3138669457854988 2213 24-25 -1.2777095252101702 2213 26-27 -1.3116135751579137 2213 28-29 -1.4160200575314263 2213 30-31 -1.439030057117641 2213 32-33 -1.304970376942613 2213 34-35 -1.2396417191656113 2213 36-37 -1.4033310852577259 2213 38-39 -1.413099872904695 2213 40-41 -1.394645515726097 2213 42-43 -1.4660328717276911 2213 44-45 -1.372954737399219 2213 46-47 -1.313280170654032 2213 48-49 -1.5645274504209041 2213 50-51 -1.4670845985477925 2213 52-53 -1.734524315579968 2213 54-55 -1.382545419057557 2213 56-57 -1.3361452140944294 2213 58-59 -1.3310976896200302 2213 60-61 -1.6188270703958523 2213 62-63 -1.341912870621087 2213 64-65 -1.4064636127319332 2213 66-67 -1.4562392622501434 2213 68-69 -1.5403459820013765 2213 70-71 -1.3819956896932553 2213 72-73 -1.5302293691944584 2213 74-75 -1.563806848923381 2213 76-77 -1.748792220141297 2213 78-79 -1.6913967577129867 2213 80-81 -1.658324481900177 2213 82-83 -1.5423091036523857 2213 84-85 -1.5239046991679004 2213 86-87 -1.3646871470417778 2213 88-89 -1.440477838781586 2213 90-91 -1.19678780899946 2213 92-93 -1.3876490144733467 2213 94-95 -1.530673231578426 2213 96-97 -1.5466282084543579 2213 98-99 -1.377455589646118 2213 100 -1.6232565250095021 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 12.0 4 24.0 5 32.0 6 36.0 7 37.0 8 42.0 9 65.0 10 347.0 11 1624.0 12 4711.0 13 8579.0 14 11139.0 15 12808.0 16 13858.0 17 14142.0 18 14646.0 19 15569.0 20 17078.0 21 19068.0 22 20703.0 23 22464.0 24 23129.0 25 24783.0 26 27695.0 27 31163.0 28 36460.0 29 43923.0 30 53126.0 31 65723.0 32 83480.0 33 111988.0 34 154974.0 35 221235.0 36 340611.0 37 588050.0 38 609963.0 39 4658.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.68067594584551 21.46683537900928 10.623834833616373 30.228653841528835 2 12.424533596712738 23.184684249299654 36.94261669930481 27.448165454682794 3 14.603338713222403 28.233947805709665 29.132426489070024 28.03028699199791 4 10.400927544800922 19.256332025316553 38.329510906427146 32.01322952345539 5 11.67300713008148 38.66622404872807 35.238672470051 14.422096351139452 6 28.76186272686063 40.43238654898395 18.25303016823206 12.552720555923363 7 25.244906925549294 33.48246654706745 24.129392027052678 17.14323450033057 8 23.837622148833656 37.35089524466735 21.339578378578206 17.47190422792079 9 24.43417743502499 17.373674516718694 22.4592713649402 35.732876683316114 10-11 22.886292295959223 28.060050880590804 29.25067904005003 19.802977783399943 12-13 23.497480285307102 25.56256043214253 29.815404473220404 21.12455480932997 14-15 21.153776912793703 26.815473365381305 26.890295172607132 25.140454549217868 16-17 20.618669237748936 30.38346180081133 27.61482512835036 21.383043833089374 18-19 20.51361819763905 29.4108021530382 29.566350692653025 20.50922895666972 20-21 21.866650239857087 28.736573008185175 28.624883536486767 20.771893215470975 22-23 21.000706865061154 28.733313980528102 29.055253285613524 21.21072586879722 24-25 21.168244735855318 28.37721820935538 28.88386350144255 21.570673553346747 26-27 21.070159567301214 28.592535560987812 28.79797124323908 21.539333628471887 28-29 20.78006451739579 28.54912088407081 29.190310147803277 21.480504450730123 30-31 21.500259117429863 28.808236931125585 28.53999456353921 21.15150938790534 32-33 20.639202158811862 28.812501431438843 29.264364572990488 21.28393183675881 34-35 20.911287645131335 28.251148780968972 29.224044767573343 21.613518806326354 36-37 21.365051869815424 28.761341108993975 28.610639567334527 21.262967453856074 38-39 21.01241449767583 28.669096021090073 28.967809225426265 21.350680255807823 40-41 21.389917857449436 28.50274135701695 28.627442979658564 21.479897805875044 42-43 20.940499786549253 28.69428579457377 28.895283643128828 21.469930775748143 44-45 21.315727831235396 28.176149461735804 28.84611424085126 21.662008466177536 46-47 21.31214501589663 28.74782464413286 28.591947800313637 21.348082539656872 48-49 21.134778341737544 28.504944925690396 28.782501427438937 21.57777530513312 50-51 21.496656965356525 28.48593149613529 28.308043129795752 21.70936840871243 52-53 20.987029967968123 28.751318939812066 28.531144305539325 21.730506786680483 54-55 21.4024776306562 28.4959865987028 28.315630425580125 21.78590534506088 56-57 21.45589238214379 28.49664964621627 28.28131306996966 21.766144901670277 58-59 21.034007053066617 28.210168373149997 28.813419035734423 21.942405538048966 60-61 21.62514455361996 28.453387905693294 28.1557729826782 21.765694558008537 62-63 21.03583922646127 28.77394065178399 28.511998189039762 21.678221932714973 64-65 21.121994554321557 28.114657614276954 28.610736013567035 22.15261181783445 66-67 21.295794559023413 28.47481757634825 28.332120287673728 21.89726757695461 68-69 21.35528242927525 28.378590140656357 28.483816741694685 21.782310688373713 70-71 21.573921848710377 28.25106746699419 28.159760055750848 22.015250628544585 72-73 21.13885791188079 28.618308653432827 28.530527095529628 21.712306339156754 74-75 21.480803296594566 28.07185562806972 28.30145529265054 22.14588578268518 76-77 21.384313409199535 28.555580985878215 28.278277112536365 21.781828492385884 78-79 21.32869549015241 28.409285361902096 28.462641988392818 21.799377159552673 80-81 21.566987568463915 28.225717505685477 28.192013674016003 22.015281251834608 82-83 21.02628596109218 28.579729693575857 28.47687166863856 21.9171126766934 84-85 21.213281333307158 27.977288291794522 28.424165922736773 22.385264452161554 86-87 21.324757428899353 28.44281970102129 28.204093283266808 22.028329586812557 88-89 21.076623826059976 28.126076644998072 28.532868010974035 22.264431517967918 90-91 21.341398983087583 28.16102916140814 28.239327221798888 22.25824463370539 92-93 20.904820249644608 28.55022974741065 28.436751425119127 22.108198577825615 94-95 20.940126443632813 27.940505401155118 28.572819796171277 22.546548359040795 96-97 21.14940621819576 28.273556116345205 28.357142087297394 22.219895578161637 98-99 20.957486283352413 28.12592140382103 28.465209716321176 22.451382596505383 100 21.253275378402538 27.84318258885909 28.25699774962237 22.646544283116 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 15.0 1 12.0 2 11.5 3 13.0 4 11.0 5 20.5 6 56.5 7 87.5 8 110.5 9 154.0 10 159.5 11 156.5 12 181.5 13 212.0 14 268.5 15 323.0 16 396.5 17 494.0 18 643.5 19 861.5 20 1113.0 21 1528.0 22 2227.5 23 3334.0 24 4890.5 25 7246.5 26 10622.0 27 15529.0 28 22362.5 29 30802.0 30 40206.0 31 50201.5 32 60846.5 33 71321.5 34 80798.0 35 89623.0 36 97398.5 37 104270.5 38 110008.5 39 113746.5 40 115929.0 41 115135.0 42 112318.0 43 108931.5 44 103784.0 45 98130.5 46 92925.5 47 88106.5 48 83335.0 49 79563.0 50 79795.0 51 75520.5 52 65499.0 53 58590.0 54 53934.5 55 49971.5 56 46185.0 57 41699.0 58 37004.0 59 32642.5 60 28199.0 61 23620.5 62 19140.0 63 15317.0 64 12208.5 65 9818.5 66 7666.5 67 5714.5 68 4242.0 69 3076.0 70 2262.0 71 1658.5 72 1240.5 73 916.5 74 609.0 75 422.0 76 257.0 77 146.5 78 82.5 79 36.0 80 25.5 81 12.0 82 8.0 83 4.5 84 2.5 85 3.0 86 0.5 87 0.5 88 0.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.769415234413943 2 5.2192365741102495 3 0.0 4 0.0046575238062978195 5 0.16505340563144666 6 0.0567371081858098 7 0.03618241634644587 8 0.042918504496050155 9 0.040955415949594044 10-11 0.04166751669683792 12-13 0.039492722522822835 14-15 0.04274529080077461 16-17 0.032968340000777537 18-19 0.026443957478732245 20-21 0.02182492560471788 22-23 0.021670957875584065 24-25 0.01805271624093948 26-27 0.011432103888185555 28-29 0.01955390159999415 30-31 0.02575110269763009 32-33 0.0014049555283460365 34-35 0.0032525682779517826 36-37 0.003733717431494946 38-39 0.005292640688974794 40-41 0.005619822113384146 42-43 0.006081725300785582 44-45 0.00764064855826543 46-47 0.014761656030704246 48-49 0.024557852796843048 50-51 0.020458462008655295 52-53 0.026578679241724332 54-55 0.022575518284245213 56-57 0.01483863989527115 58-59 0.013606898062200654 60-61 0.01135512002361865 62-63 0.01655153088188481 64-65 0.01826442186849847 66-67 0.018880292785033722 68-69 0.026751892936999873 70-71 0.02605903815589771 72-73 0.022729486013379027 74-75 0.02309515937007183 76-77 0.016763236509443804 78-79 0.012760075551964685 80-81 0.012298172364563249 82-83 0.00737120503228126 84-85 0.00985393466456398 86-87 0.007833108219682696 88-89 0.006851563946454642 90-91 0.006986285709446729 92-93 0.0036182416346445864 94-95 0.001174003934645318 96-97 8.853144425194201E-4 98-99 3.4642739055107744E-4 100 0.10881669256532177 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2597947.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.34595633850851 #Duplication Level Percentage of deduplicated Percentage of total 1 89.63800996311946 76.50241684583185 2 7.906471004011693 13.49570658200131 3 1.4875469992315606 3.808683637436884 4 0.44066226819467497 1.5043497080548343 5 0.18258650231550996 0.7791509827310248 6 0.09898485334324761 0.5068774182963887 7 0.05938033731562964 0.3547510173133944 8 0.03716917128749893 0.2537790775873142 9 0.027115329896331895 0.20827653852929368 >10 0.11583983612701045 1.6067975894708824 >50 0.003969991578810337 0.22461669999106326 >100 0.0017668409377583018 0.3007058902950112 >500 3.6138373872118525E-4 0.21690087132821523 >1k 1.3551890201497584E-4 0.23698714113251704 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 2753 0.10596828957634624 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 3.849193228345305E-5 6 0.0 0.0 0.0 0.0 3.849193228345305E-5 7 0.0 0.0 0.0 3.849193228345305E-5 3.849193228345305E-5 8 0.0 0.0 0.0 3.849193228345305E-5 3.849193228345305E-5 9 3.849193228345305E-5 0.0 0.0 3.849193228345305E-5 3.849193228345305E-5 10-11 7.69838645669061E-5 0.0 0.0 7.69838645669061E-5 3.849193228345305E-5 12-13 7.69838645669061E-5 0.0 0.0 7.69838645669061E-5 3.849193228345305E-5 14-15 7.69838645669061E-5 0.0 0.0 1.1547579685035915E-4 3.849193228345305E-5 16-17 7.69838645669061E-5 0.0 0.0 1.3472176299208566E-4 3.849193228345305E-5 18-19 7.69838645669061E-5 0.0 0.0 1.539677291338122E-4 3.849193228345305E-5 20-21 7.69838645669061E-5 0.0 0.0 1.9245966141726524E-4 3.849193228345305E-5 22-23 7.69838645669061E-5 0.0 0.0 2.309515937007183E-4 3.849193228345305E-5 24-25 7.69838645669061E-5 0.0 0.0 2.309515937007183E-4 3.849193228345305E-5 26-27 7.69838645669061E-5 0.0 0.0 2.694435259841713E-4 3.849193228345305E-5 28-29 7.69838645669061E-5 0.0 0.0 5.003951196848897E-4 3.849193228345305E-5 30-31 7.69838645669061E-5 0.0 0.0 0.0015781692236215752 3.849193228345305E-5 32-33 7.69838645669061E-5 0.0 0.0 0.005311886655116521 3.849193228345305E-5 34-35 7.69838645669061E-5 0.0 0.0 0.01052754347952441 3.849193228345305E-5 36-37 7.69838645669061E-5 0.0 0.0 0.019168982277159616 3.849193228345305E-5 38-39 7.69838645669061E-5 0.0 0.0 0.035085396276367455 3.849193228345305E-5 40-41 7.69838645669061E-5 0.0 0.0 0.0552936607251803 3.849193228345305E-5 42-43 7.69838645669061E-5 0.0 0.0 0.07632950171808739 3.849193228345305E-5 44-45 7.69838645669061E-5 0.0 0.0 0.10177266895744987 3.849193228345305E-5 46-47 7.69838645669061E-5 0.0 0.0 0.13297038007318857 3.849193228345305E-5 48-49 7.69838645669061E-5 0.0 0.0 0.16840220374010711 3.849193228345305E-5 50-51 7.69838645669061E-5 0.0 0.0 0.20777945046607957 3.849193228345305E-5 52-53 7.69838645669061E-5 0.0 0.0 0.24990887035031895 3.849193228345305E-5 54-55 7.69838645669061E-5 0.0 0.0 0.29519462868180146 3.849193228345305E-5 56-57 7.69838645669061E-5 0.0 0.0 0.3499878942872968 3.849193228345305E-5 58-59 7.69838645669061E-5 0.0 0.0 0.41369204221641165 3.849193228345305E-5 60-61 7.69838645669061E-5 0.0 0.0 0.48524854433135084 3.849193228345305E-5 62-63 7.69838645669061E-5 0.0 0.0 0.5594609897738483 3.849193228345305E-5 64-65 7.69838645669061E-5 0.0 0.0 0.6422571361155558 3.849193228345305E-5 66-67 7.69838645669061E-5 0.0 0.0 0.7381597853997791 3.849193228345305E-5 68-69 7.69838645669061E-5 0.0 0.0 0.8479195303060455 3.849193228345305E-5 70-71 7.69838645669061E-5 0.0 0.0 0.9718635522587644 3.849193228345305E-5 72-73 7.69838645669061E-5 0.0 0.0 1.104333537212268 3.849193228345305E-5 74-75 7.69838645669061E-5 0.0 0.0 1.24325092082325 3.849193228345305E-5 76-77 7.69838645669061E-5 0.0 0.0 1.396314089548401 3.849193228345305E-5 78-79 7.69838645669061E-5 0.0 0.0 1.5737426514089778 3.849193228345305E-5 80-81 7.69838645669061E-5 0.0 0.0 1.7728806630774223 3.849193228345305E-5 82-83 7.69838645669061E-5 0.0 0.0 1.992111463397829 3.849193228345305E-5 84-85 7.69838645669061E-5 0.0 0.0 2.2183285494276825 3.849193228345305E-5 86-87 7.69838645669061E-5 0.0 0.0 2.460539033321311 3.849193228345305E-5 88 7.69838645669061E-5 0.0 0.0 2.6596000611251887 3.849193228345305E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 7030 0.0 22.237968 1 CAACGCA 7665 0.0 19.689804 5 AACGCAG 7770 0.0 19.45985 6 TCAACGC 7765 0.0 19.436234 4 ATCAACG 7750 0.0 19.412811 3 TATCAAC 7935 0.0 19.128275 2 ACGCAGA 8550 0.0 17.73846 7 CGCAGAG 8605 0.0 17.570517 8 GAGTACT 2915 0.0 16.429186 12-13 GTACATG 9180 0.0 16.326916 1 CATGGGG 3920 0.0 16.191841 4 TACATGG 9275 0.0 16.15081 2 TAATACG 295 1.3705394E-6 15.937733 4 GCAGAGT 9740 0.0 15.377812 9 GTACTAG 555 7.2759576E-12 15.201829 1 ACATGGG 9440 0.0 15.140554 3 GTATATA 1985 0.0 14.751336 1 TATACTG 1560 0.0 14.165172 5 AGTACTT 3890 0.0 14.061472 12-13 ACCGATC 205 0.0067136995 13.742854 8 >>END_MODULE