FastQCFastQC Report
Wed 25 May 2016
SRR1295138_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295138_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2476603
Sequences flagged as poor quality0
Sequence length100
%GC43

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG1403.4686623E-421.3960295
GTATAAG9750.019.0634521
GTACATG75500.018.4637761
GTATAGA10500.017.7017781
TACATGG75650.016.7852132
GTATAGG6750.016.521661
CATGGGG33750.015.6055914
TATAGGA10550.015.2652122
ACATGGG77750.015.2057633
GTATTAG9900.015.01969051
AGGGTCG2356.4487744E-414.8709985
GTATATA19000.014.6738431
GTATCAA53650.013.8578691
GTCCTGG14800.013.8145651
TACCGTC2200.00721636913.5935717
TTATACT12900.013.4647454
TACACCG2600.001385171813.4410945
GTATTAA14300.013.431071
GTAGTGC4553.1845047E-713.2312716
GTATTAT14050.013.2290871
Produced by FastQC (version 0.11.5)