##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295138_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2476603
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.278613084131774	33.0	27.0	33.0	6.0	33.0
2	22.51589172749932	27.0	6.0	33.0	2.0	33.0
3	24.849372305533024	27.0	15.0	33.0	15.0	33.0
4	25.01670594762261	33.0	6.0	37.0	2.0	37.0
5	27.027080238536414	33.0	15.0	37.0	2.0	37.0
6	27.72323420427093	33.0	15.0	37.0	6.0	37.0
7	27.627091625100995	33.0	15.0	37.0	6.0	37.0
8	28.242195862639267	33.0	22.0	37.0	6.0	37.0
9	29.3208540084947	33.0	27.0	37.0	15.0	37.0
10-11	28.822625992135194	33.0	24.5	37.0	10.5	37.0
12-13	27.651491579393223	33.0	15.0	37.0	4.0	37.0
14-15	29.3875905827458	33.0	24.5	37.0	10.5	40.0
16-17	29.803495352303138	35.0	27.0	38.5	6.0	40.0
18-19	29.965362433946822	35.0	27.0	40.0	10.5	40.0
20-21	29.499589356873102	33.0	24.5	40.0	6.0	40.0
22-23	30.247763367806627	35.0	27.0	40.0	15.0	40.0
24-25	30.390378877841947	33.0	27.0	40.0	15.0	40.0
26-27	28.958832925583955	33.0	22.0	38.5	4.0	40.0
28-29	29.65722826791375	33.0	27.0	40.0	10.5	40.0
30-31	30.318237117535592	33.0	27.0	40.0	15.0	40.0
32-33	29.145486176024175	33.0	22.0	37.0	6.0	40.0
34-35	28.75390726733352	33.0	22.0	37.0	6.0	40.0
36-37	29.09992134387304	33.0	22.0	37.0	6.0	40.0
38-39	29.18034077322849	33.0	24.5	37.0	6.0	40.0
40-41	28.216017060465482	33.0	22.0	37.0	6.0	40.0
42-43	28.882702839332747	33.0	22.0	37.0	6.0	40.0
44-45	28.787696695837	33.0	22.0	37.0	6.0	40.0
46-47	28.494913597375117	33.0	22.0	37.0	6.0	40.0
48-49	28.42964051969573	33.0	22.0	37.0	6.0	40.0
50-51	28.410180396292827	33.0	22.0	37.0	6.0	40.0
52-53	28.590575679670906	33.0	22.0	37.0	6.0	40.0
54-55	28.583327444891246	33.0	22.0	37.0	6.0	40.0
56-57	28.569650040801857	33.0	22.0	37.0	6.0	40.0
58-59	28.428670844701394	33.0	22.0	37.0	6.0	40.0
60-61	28.387328934027778	33.0	22.0	37.0	6.0	40.0
62-63	28.333243963606602	33.0	22.0	37.0	6.0	40.0
64-65	27.905387944696827	33.0	22.0	37.0	6.0	40.0
66-67	28.106312557967506	33.0	22.0	37.0	10.5	40.0
68-69	27.70434017886597	33.0	22.0	37.0	6.0	38.5
70-71	27.821637541422668	33.0	22.0	37.0	10.5	37.0
72-73	27.86297521241798	33.0	22.0	37.0	10.5	37.0
74-75	27.938193364055522	33.0	22.0	37.0	15.0	37.0
76-77	27.688921882110293	33.0	22.0	37.0	10.5	37.0
78-79	27.26247929119039	33.0	22.0	37.0	6.0	37.0
80-81	27.07587913767366	27.0	22.0	35.0	6.0	37.0
82-83	27.166640959410934	27.0	22.0	33.0	15.0	37.0
84-85	27.13503981057925	27.0	22.0	33.0	10.5	37.0
86-87	26.92172362708113	27.0	22.0	33.0	15.0	37.0
88-89	26.852286983420434	27.0	22.0	33.0	15.0	37.0
90-91	26.993887797115647	27.0	22.0	33.0	15.0	37.0
92-93	26.92742498494914	27.0	22.0	33.0	15.0	37.0
94-95	26.80143486864871	27.0	22.0	33.0	15.0	37.0
96-97	26.888284072982223	27.0	22.0	33.0	15.0	37.0
98-99	26.95163940284333	27.0	22.0	33.0	15.0	37.0
100	26.623305390488504	27.0	22.0	33.0	15.0	37.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	7.375358073700685
1101	2	11.274952455641532
1101	3	8.486395427703414
1101	4	13.007633457902603
1101	5	10.939260975001542
1101	6	10.180322362035845
1101	7	9.535883976347968
1101	8	9.08624012974726
1101	9	7.41732576648689
1101	10-11	7.830942687792984
1101	12-13	9.337259089716856
1101	14-15	8.137628683469199
1101	16-17	8.30844387848959
1101	18-19	8.334011213413202
1101	20-21	7.392313785832723
1101	22-23	7.568742054131782
1101	24-25	7.474591515114469
1101	26-27	8.637909219115226
1101	28-29	8.316119677575536
1101	30-31	5.673259249700781
1101	32-33	7.210566756715277
1101	34-35	8.874811601125138
1101	36-37	8.23430326653655
1101	38-39	8.286170271189857
1101	40-41	9.084528286231652
1101	42-43	8.795347070141442
1101	44-45	8.703792299362568
1101	46-47	7.597018983740146
1101	48-49	5.671941758428257
1101	50-51	7.161960140407931
1101	52-53	6.15202250956866
1101	54-55	4.1522270668056365
1101	56-57	6.335167324479574
1101	58-59	6.479158662867892
1101	60-61	7.046049139039386
1101	62-63	6.475679377914563
1101	64-65	6.911836662091673
1101	66-67	6.110474338098648
1101	68-69	6.993677135711096
1101	70-71	6.484284259003655
1101	72-73	5.680726901120224
1101	74-75	5.115029712072278
1101	76-77	5.326064128732501
1101	78-79	5.32012939694382
1101	80-81	5.130255955757658
1101	82-83	4.757848620075354
1101	84-85	4.528708675697619
1101	86-87	4.288291934026358
1101	88-89	4.246661180813646
1101	90-91	4.204160300039742
1101	92-93	4.013840287110838
1101	94-95	3.9597241699348054
1101	96-97	3.9056544913801794
1101	98-99	3.806767776465243
1101	100	4.0143451954932345
1102	1	6.685130480616916
1102	2	10.379747456180493
1102	3	7.929974088825539
1102	4	12.490734693487465
1102	5	10.541970362651096
1102	6	9.424663618230795
1102	7	6.559035582023846
1102	8	7.84011582305623
1102	9	6.2026985495453815
1102	10-11	7.345492871935626
1102	12-13	8.895816594904527
1102	14-15	6.0498386198663106
1102	16-17	7.2985980915778725
1102	18-19	7.30538236690786
1102	20-21	6.818844124363512
1102	22-23	6.466310520762114
1102	24-25	6.8206071750271775
1102	26-27	8.38895629865068
1102	28-29	7.575916703008147
1102	30-31	4.2896041043895075
1102	32-33	4.602976810222643
1102	34-35	5.043899214923748
1102	36-37	5.188351861502955
1102	38-39	4.529176186525877
1102	40-41	6.2583257075454455
1102	42-43	6.808762300705023
1102	44-45	6.440977369128355
1102	46-47	5.545830871533749
1102	48-49	3.8630105990229495
1102	50-51	4.968862270915846
1102	52-53	5.815816142994269
1102	54-55	5.4155422243407045
1102	56-57	6.334318633520251
1102	58-59	6.15840554992128
1102	60-61	6.128233596788295
1102	62-63	4.456293019935977
1102	64-65	6.152862344469803
1102	66-67	3.842130200737799
1102	68-69	6.154355375837291
1102	70-71	5.3745880578364655
1102	72-73	5.03763849445652
1102	74-75	4.277876132228478
1102	76-77	3.3805437958504605
1102	78-79	4.4082037244627195
1102	80-81	4.471419348823286
1102	82-83	3.9029881102983843
1102	84-85	3.053135592199496
1102	86-87	3.2977543488850216
1102	88-89	3.369458540374385
1102	90-91	3.376886726294053
1102	92-93	3.165327832705408
1102	94-95	3.0459618610212225
1102	96-97	3.3157141671601096
1102	98-99	3.1242517337663145
1102	100	3.184148517531824
1103	1	6.685024119986167
1103	2	10.849271473875554
1103	3	7.915925558248215
1103	4	11.968523036808701
1103	5	10.351750686450519
1103	6	9.104835457953065
1103	7	8.21425528751719
1103	8	8.1670073753858
1103	9	7.154294279832122
1103	10-11	7.625219412825672
1103	12-13	8.905042525249687
1103	14-15	8.687790066691715
1103	16-17	8.15447103268017
1103	18-19	7.430569980086037
1103	20-21	8.454786750161286
1103	22-23	6.980665335083657
1103	24-25	5.4370574193148755
1103	26-27	5.998805871938803
1103	28-29	7.071871372316551
1103	30-31	6.23959239425842
1103	32-33	8.015523821621432
1103	34-35	7.292209894256416
1103	36-37	4.277793793899164
1103	38-39	6.500395209079354
1103	40-41	6.792232430457936
1103	42-43	7.658908611086122
1103	44-45	5.899203167795562
1103	46-47	6.700606094207576
1103	48-49	5.932411294182085
1103	50-51	6.575956127442115
1103	52-53	6.220415260361442
1103	54-55	5.170037756915235
1103	56-57	6.157028225338141
1103	58-59	5.7637904875263
1103	60-61	5.275270333819904
1103	62-63	2.974807755451611
1103	64-65	5.325810379385665
1103	66-67	4.292135089839583
1103	68-69	5.362436489174343
1103	70-71	3.8852533788028616
1103	72-73	4.63515525619448
1103	74-75	3.40048751656823
1103	76-77	3.7967094051413426
1103	78-79	3.101018181585502
1103	80-81	4.415605927827404
1103	82-83	3.6183885089031556
1103	84-85	3.437152637240331
1103	86-87	3.116721580883432
1103	88-89	3.3937443688420963
1103	90-91	3.025918091311741
1103	92-93	2.4675423935524776
1103	94-95	3.1351820750927395
1103	96-97	3.3226562383482197
1103	98-99	3.1182124027907214
1103	100	3.288797404699725
1104	1	6.32547693674978
1104	2	9.57690446745401
1104	3	7.257509491933757
1104	4	12.078387241586615
1104	5	9.37871021826474
1104	6	8.032839157901481
1104	7	3.819503023967016
1104	8	6.552763570131162
1104	9	6.496166667188255
1104	10-11	7.019161956895971
1104	12-13	7.418477251400137
1104	14-15	7.163720230165399
1104	16-17	7.02416333216253
1104	18-19	6.730427603224943
1104	20-21	7.410815816102197
1104	22-23	6.310663058192318
1104	24-25	5.9474936930399025
1104	26-27	8.140410511104886
1104	28-29	7.025457440587715
1104	30-31	6.2607616491101545
1104	32-33	7.397579676611915
1104	34-35	7.868972546123295
1104	36-37	6.455346455905321
1104	38-39	7.1421385251581135
1104	40-41	6.9936475184206515
1104	42-43	7.21756560096463
1104	44-45	6.665752432252937
1104	46-47	6.509988345128114
1104	48-49	5.316873467546014
1104	50-51	5.57508454055559
1104	52-53	5.874931332012366
1104	54-55	4.764525331848084
1104	56-57	5.894265146554144
1104	58-59	3.821308533668926
1104	60-61	4.244507610800181
1104	62-63	3.8609174731526643
1104	64-65	2.76316335090673
1104	66-67	3.1401849324138915
1104	68-69	4.072357829694116
1104	70-71	2.5814285927061285
1104	72-73	2.4247911314635253
1104	74-75	3.1533907308984155
1104	76-77	3.7630172479646866
1104	78-79	4.252759149620083
1104	80-81	2.8526140214645572
1104	82-83	2.858882950086425
1104	84-85	3.0238869702712634
1104	86-87	0.8061885748532305
1104	88-89	0.348200494213426
1104	90-91	2.301541621560599
1104	92-93	2.489710349864623
1104	94-95	2.3954487915664053
1104	96-97	2.807313406108861
1104	98-99	2.511445517329033
1104	100	0.655268045253294
1105	1	5.626554653615308
1105	2	-2.8688169127459275
1105	3	0.3870251320498461
1105	4	8.486892669943064
1105	5	8.558388171613117
1105	6	7.931586228738844
1105	7	5.356990071156094
1105	8	5.887999085556295
1105	9	4.8048187149147985
1105	10-11	5.019706189939079
1105	12-13	5.07215455979528
1105	14-15	5.856242104829487
1105	16-17	5.982453346136516
1105	18-19	6.090481994281593
1105	20-21	6.986401756121541
1105	22-23	1.9863657347482686
1105	24-25	3.991316760867491
1105	26-27	6.892660645470155
1105	28-29	5.996689086293401
1105	30-31	5.27610637661741
1105	32-33	7.10110208873704
1105	34-35	5.986639535266576
1105	36-37	6.1108390926891545
1105	38-39	4.100119176560888
1105	40-41	5.434347507671433
1105	42-43	5.57055065954788
1105	44-45	6.2492577531171705
1105	46-47	5.316961579568616
1105	48-49	5.114031373597822
1105	50-51	5.876238146919128
1105	52-53	5.532421388983238
1105	54-55	5.201261116003735
1105	56-57	5.10101221739151
1105	58-59	1.7036687400539599
1105	60-61	4.248285101932069
1105	62-63	-1.0563936092938597
1105	64-65	-0.5114951463522175
1105	66-67	0.7695236403575656
1105	68-69	2.9538899161178485
1105	70-71	3.0199120926523797
1105	72-73	2.7527616785988585
1105	74-75	2.7658797129472177
1105	76-77	1.8219496353666003
1105	78-79	3.374451291939767
1105	80-81	1.7846245557008267
1105	82-83	1.0613919256856974
1105	84-85	1.431624151825602
1105	86-87	-0.27523075224555527
1105	88-89	1.5851332040919566
1105	90-91	1.3706373971168695
1105	92-93	1.6936640207234852
1105	94-95	1.0837159097003415
1105	96-97	2.0099122451930285
1105	98-99	1.8761908682910864
1105	100	2.076511730086146
1106	1	6.270357796555285
1106	2	10.31647939625908
1106	3	6.867736769069385
1106	4	11.960412016540108
1106	5	9.973152235175188
1106	6	9.373117483784696
1106	7	6.593095603824363
1106	8	7.245135106266446
1106	9	6.833511426977012
1106	10-11	5.757267034438932
1106	12-13	8.032753884475138
1106	14-15	7.869257053152321
1106	16-17	7.7023226148350545
1106	18-19	7.323948221099943
1106	20-21	8.341695741336498
1106	22-23	6.299813074503234
1106	24-25	5.073305068358408
1106	26-27	7.6969957642767035
1106	28-29	6.157025897928065
1106	30-31	5.314120057165102
1106	32-33	7.364553235300345
1106	34-35	6.804222120717064
1106	36-37	5.840014074776601
1106	38-39	7.040488989357865
1106	40-41	6.550711677615155
1106	42-43	6.238428051480849
1106	44-45	4.524217354974475
1106	46-47	6.03879565594357
1106	48-49	5.934135438433241
1106	50-51	6.641133808327869
1106	52-53	5.725039865705423
1106	54-55	5.316707873000077
1106	56-57	4.911718933771677
1106	58-59	4.948639486490713
1106	60-61	4.829440683158911
1106	62-63	4.9823756687249094
1106	64-65	5.118066005617941
1106	66-67	3.06022148649118
1106	68-69	3.2468861445564414
1106	70-71	2.9599800426139957
1106	72-73	2.9133361980737646
1106	74-75	2.8103922561891466
1106	76-77	3.5062353116389637
1106	78-79	4.212935519075476
1106	80-81	3.6537585008819526
1106	82-83	2.1524402454726435
1106	84-85	2.7263695616784958
1106	86-87	3.235194155830296
1106	88-89	3.3258384095906486
1106	90-91	3.202138830190396
1106	92-93	2.943373181262686
1106	94-95	1.488673749907452
1106	96-97	1.4458240346520768
1106	98-99	2.023858844667
1106	100	2.8966039530657817
1107	1	6.193673703435319
1107	2	10.500657125188173
1107	3	6.154516222763473
1107	4	11.484270364188365
1107	5	9.47621641440237
1107	6	8.065773422228084
1107	7	3.891611472101882
1107	8	6.402779547988622
1107	9	5.846790055248896
1107	10-11	4.605342494104249
1107	12-13	6.186961242090202
1107	14-15	6.195974366165903
1107	16-17	7.135179750354645
1107	18-19	6.491597257876766
1107	20-21	5.71072925889014
1107	22-23	5.964968147202825
1107	24-25	4.81608298661731
1107	26-27	7.336698615778726
1107	28-29	6.557984458264009
1107	30-31	5.25035389922024
1107	32-33	5.06172108694091
1107	34-35	6.867588259779957
1107	36-37	6.65846037677089
1107	38-39	6.561699196982982
1107	40-41	7.868071497787824
1107	42-43	6.242046949646767
1107	44-45	4.350670970341881
1107	46-47	3.7706839480720653
1107	48-49	4.156232466099201
1107	50-51	4.9899689841612975
1107	52-53	4.056922902748987
1107	54-55	5.457383858614143
1107	56-57	5.514292950542316
1107	58-59	3.2436730049528144
1107	60-61	3.0525945454419343
1107	62-63	0.5753522721713473
1107	64-65	2.2746245070997233
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2206	7	8.080847670259715
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2206	74-75	1.5933741515614095
2206	76-77	2.239996404103035
2206	78-79	0.9354398954242704
2206	80-81	2.0554703486537846
2206	82-83	2.9110121270597666
2206	84-85	3.357649752762775
2206	86-87	-1.6602849735681886
2206	88-89	-1.1817791140886555
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2206	92-93	1.983345764037736
2206	94-95	2.7618178103710953
2206	96-97	1.6174719648591172
2206	98-99	1.383562725262287
2206	100	1.6749926686718304
2207	1	6.320676957785253
2207	2	10.75449138339101
2207	3	7.684310470083162
2207	4	11.99753877586129
2207	5	10.503831514049754
2207	6	9.344847466912555
2207	7	6.009471312994389
2207	8	6.924354956105763
2207	9	6.216783532377185
2207	10-11	7.160511803074094
2207	12-13	8.862182912571289
2207	14-15	8.301026953627836
2207	16-17	7.43600220045419
2207	18-19	4.3443484046763885
2207	20-21	7.634605671746268
2207	22-23	6.5712353281825955
2207	24-25	4.393352913149819
2207	26-27	4.065758674445323
2207	28-29	5.236437588361902
2207	30-31	5.685446879580038
2207	32-33	6.939687115280375
2207	34-35	6.45236930140188
2207	36-37	6.876585602916968
2207	38-39	7.067938214884915
2207	40-41	4.330862264285869
2207	42-43	5.798029958988344
2207	44-45	4.272308925639905
2207	46-47	5.726824452514194
2207	48-49	2.6894424244495916
2207	50-51	5.589946468982401
2207	52-53	3.1126389915532684
2207	54-55	3.326749727735649
2207	56-57	5.408576787057942
2207	58-59	3.865263307406142
2207	60-61	3.7809051286145916
2207	62-63	4.530069217782046
2207	64-65	3.5691414740168277
2207	66-67	3.621293133618174
2207	68-69	3.629359644003493
2207	70-71	3.766741206100466
2207	72-73	3.975604152420111
2207	74-75	3.6446602215770696
2207	76-77	4.17825208270477
2207	78-79	4.330456396602809
2207	80-81	4.3065959835481245
2207	82-83	3.9999384574356966
2207	84-85	3.420188881869695
2207	86-87	3.673256545719102
2207	88-89	3.5340980514693463
2207	90-91	1.6078021984583906
2207	92-93	-0.4306937415344976
2207	94-95	1.5059520126229096
2207	96-97	-0.7813707199987867
2207	98-99	0.8825125941873857
2207	100	2.344341695696354
2208	1	1.1499708011686138
2208	2	8.51703895592432
2208	3	6.544719801444558
2208	4	11.649937327875005
2208	5	9.923401774144057
2208	6	9.029981043757992
2208	7	7.520768332567236
2208	8	7.777381793891259
2208	9	6.458582915421296
2208	10-11	6.57795995535232
2208	12-13	6.88011725105325
2208	14-15	7.807539744348784
2208	16-17	7.070506387031536
2208	18-19	6.387029159017533
2208	20-21	6.579955898141204
2208	22-23	5.809734647199441
2208	24-25	5.978404291334954
2208	26-27	7.922859679007342
2208	28-29	6.947563449554771
2208	30-31	5.980777293758113
2208	32-33	8.237892638948555
2208	34-35	6.423977896870404
2208	36-37	6.704356749806159
2208	38-39	7.143600033600503
2208	40-41	7.490347174134328
2208	42-43	6.902529221087601
2208	44-45	5.93701406384055
2208	46-47	4.428367459002768
2208	48-49	4.609584223675089
2208	50-51	5.861106984207975
2208	52-53	5.426661522540591
2208	54-55	6.213591214547911
2208	56-57	6.234904513136687
2208	58-59	5.560638380778048
2208	60-61	5.065523420219922
2208	62-63	3.291590262392198
2208	64-65	3.2778295492746246
2208	66-67	2.940060104773856
2208	68-69	5.456991698102158
2208	70-71	5.24412117190499
2208	72-73	4.47106225910963
2208	74-75	2.0472002547079384
2208	76-77	3.2349564038127845
2208	78-79	3.943364764002059
2208	80-81	3.4066663495165237
2208	82-83	1.3413186413908136
2208	84-85	1.9422104433137335
2208	86-87	1.130724152551803
2208	88-89	0.6838317002562135
2208	90-91	1.8630567935736764
2208	92-93	0.7461271010852357
2208	94-95	-1.2684142570586197
2208	96-97	1.2757706565012796
2208	98-99	0.1195775428946746
2208	100	1.7162766220586292
2209	1	5.2854465519214315
2209	2	9.612571272548141
2209	3	5.623646258268195
2209	4	-3.501785377461001
2209	5	0.4929650632259772
2209	6	4.340658856129611
2209	7	2.353107813557269
2209	8	5.245797969355969
2209	9	5.132672757996676
2209	10-11	5.238109769742042
2209	12-13	7.4445490260402245
2209	14-15	6.75508727058622
2209	16-17	6.3284062723618675
2209	18-19	5.4882799703345775
2209	20-21	6.042331666046824
2209	22-23	5.601063425025103
2209	24-25	5.2232014726633835
2209	26-27	7.020685670296469
2209	28-29	6.247926848118169
2209	30-31	4.143063317161101
2209	32-33	6.286847499147907
2209	34-35	5.689938839035804
2209	36-37	6.302989243834439
2209	38-39	6.812862258546826
2209	40-41	7.237121494057419
2209	42-43	6.83573039886053
2209	44-45	6.475910412596232
2209	46-47	6.682036517885589
2209	48-49	5.30055096616174
2209	50-51	4.681951373335213
2209	52-53	4.881122846408822
2209	54-55	5.429420853719627
2209	56-57	5.028314011448938
2209	58-59	3.14803295919495
2209	60-61	4.049587691088107
2209	62-63	4.298232672488211
2209	64-65	5.157164735181372
2209	66-67	4.531513005503591
2209	68-69	3.917454071388466
2209	70-71	3.7686546447548572
2209	72-73	0.9249475544978552
2209	74-75	2.28611740587073
2209	76-77	3.3747026369458624
2209	78-79	3.525787907925732
2209	80-81	3.6512983722397543
2209	82-83	2.9481777313710325
2209	84-85	2.9267698260298474
2209	86-87	1.5458391147203407
2209	88-89	2.0473386102156397
2209	90-91	2.386310655336903
2209	92-93	2.32608361952132
2209	94-95	0.70817262877571
2209	96-97	0.15348291053854268
2209	98-99	0.2236810170521757
2209	100	0.2514685574349116
2210	1	1.7509186786681497
2210	2	-17.036047913498372
2210	3	-7.662763467043973
2210	4	-5.9936833854532505
2210	5	-0.1961201684309941
2210	6	3.116515961924133
2210	7	-5.970291853150702
2210	8	-5.888797421230748
2210	9	-2.2779234503312615
2210	10-11	-0.8083834651478732
2210	12-13	3.3486428628130227
2210	14-15	-3.474569128494025
2210	16-17	-0.5560576829570039
2210	18-19	-0.474195238694584
2210	20-21	2.4396089978941653
2210	22-23	2.6159731874753263
2210	24-25	2.21408548858593
2210	26-27	4.03628895509571
2210	28-29	3.4081126897544713
2210	30-31	2.557425847751592
2210	32-33	4.405533083986008
2210	34-35	2.1864576745413338
2210	36-37	3.8606180424764887
2210	38-39	3.298424862216553
2210	40-41	4.145937891968956
2210	42-43	2.5210976449277673
2210	44-45	3.358126349608689
2210	46-47	2.6501960052326012
2210	48-49	2.787206766280864
2210	50-51	1.9816841553591296
2210	52-53	2.0957808401372446
2210	54-55	2.620102999679567
2210	56-57	2.872141191716697
2210	58-59	1.7949861911208593
2210	60-61	2.7452068000423573
2210	62-63	1.947625773329115
2210	64-65	2.7570106562614214
2210	66-67	2.60051216488111
2210	68-69	3.1566351711465614
2210	70-71	2.276175804644911
2210	72-73	0.8282075011598664
2210	74-75	0.13547828813007357
2210	76-77	-0.7820410043505284
2210	78-79	0.00879665437737387
2210	80-81	0.8390817699105106
2210	82-83	0.7856875263126568
2210	84-85	-0.8882609959144858
2210	86-87	-0.6668935965476628
2210	88-89	-1.7024351952767098
2210	90-91	-1.697357639185114
2210	92-93	-1.0689125381862148
2210	94-95	-0.4422281277845279
2210	96-97	-0.07482217381940615
2210	98-99	-0.22595742815529007
2210	100	-0.29114766707184003
2211	1	1.2096886733806507
2211	2	-17.036047913498372
2211	3	-7.953648768181223
2211	4	-19.048660377461
2211	5	-21.23995644840193
2211	6	-12.197336349203411
2211	7	-9.969810784472077
2211	8	-19.991980111396117
2211	9	-14.649920760949284
2211	10-11	-9.013290152877321
2211	12-13	-22.301998939076054
2211	14-15	-17.799389868543788
2211	16-17	-16.458279882936722
2211	18-19	-18.933150008229664
2211	20-21	-7.667170082585301
2211	22-23	-3.0998287521992616
2211	24-25	-2.1125965553601347
2211	26-27	-17.4140331378809
2211	28-29	-4.024524770064197
2211	30-31	-3.710190081012417
2211	32-33	-0.1945005923977341
2211	34-35	-3.527183436087359
2211	36-37	-4.529917238638561
2211	38-39	-1.402507098011423
2211	40-41	-3.2822624501225732
2211	42-43	-0.8414039185060282
2211	44-45	0.384653614593752
2211	46-47	0.6268129464948551
2211	48-49	0.3562302307524341
2211	50-51	-3.2298524374246718
2211	52-53	-0.7126334797893463
2211	54-55	-0.7139382759184869
2211	56-57	-4.6332091999042575
2211	58-59	-1.316249119004901
2211	60-61	-0.7971166434573327
2211	62-63	-0.4355908249273881
2211	64-65	-2.769232136673267
2211	66-67	-2.076001855741005
2211	68-69	0.10648994814513912
2211	70-71	-0.3406479882679463
2211	72-73	-1.4543682133678004
2211	74-75	-2.167410874481778
2211	76-77	-1.8907734017804039
2211	78-79	-1.3237029395435975
2211	80-81	-1.1476992446163585
2211	82-83	-1.7110250659673731
2211	84-85	-1.9096479033592821
2211	86-87	-2.143422228921583
2211	88-89	-2.973512272897974
2211	90-91	-2.578206290360434
2211	92-93	-2.542192459550712
2211	94-95	-2.7477389728332042
2211	96-97	-3.3495641294155973
2211	98-99	-3.1582639956333303
2211	100	-2.551713189153908
2212	1	-21.030142110869267
2212	2	-17.036047913498372
2212	3	-19.96210955568529
2212	4	-19.048660377461
2212	5	-21.23995644840193
2212	6	-21.895351318288995
2212	7	-22.464116023652032
2212	8	-22.74856976320217
2212	9	-24.219071428049837
2212	10-11	-11.365896328213145
2212	12-13	-22.301998939076054
2212	14-15	-23.746184531739363
2212	16-17	-24.12326523926604
2212	18-19	-18.62686208246665
2212	20-21	-23.84956513627876
2212	22-23	-18.891630674550974
2212	24-25	-25.41669678315057
2212	26-27	-17.358454650209833
2212	28-29	-18.260539230995928
2212	30-31	-6.204544300434076
2212	32-33	-17.5262384708218
2212	34-35	-13.965560605660059
2212	36-37	-17.8547305515458
2212	38-39	-23.9241471891276
2212	40-41	-10.447421886624925
2212	42-43	-14.391925680853898
2212	44-45	-17.429445948926386
2212	46-47	-17.37322259325836
2212	48-49	-17.965541525456747
2212	50-51	-9.199946058181126
2212	52-53	-2.358570691513485
2212	54-55	-23.868998477675724
2212	56-57	-23.809258662969665
2212	58-59	-13.054570433009088
2212	60-61	-6.503805638566288
2212	62-63	-3.394588030081195
2212	64-65	-17.636215369922347
2212	66-67	-17.9673042313215
2212	68-69	-14.968340229990442
2212	70-71	-9.997365764115234
2212	72-73	-17.936098841682206
2212	74-75	-6.809431993107786
2212	76-77	-11.435658042326171
2212	78-79	-4.859950256938408
2212	80-81	-2.491946211524585
2212	82-83	-2.9244349514983554
2212	84-85	-5.426935602443287
2212	86-87	-5.510806092116024
2212	88-89	-3.6170958483108784
2212	90-91	-4.51911634583605
2212	92-93	-5.824926058471547
2212	94-95	-3.887099262444714
2212	96-97	-5.598631372437982
2212	98-99	-4.2539318418751755
2212	100	-4.016919253812478
2213	1	-21.030142110869267
2213	2	-17.036047913498372
2213	3	-19.96210955568529
2213	4	-19.048660377461
2213	5	-21.23995644840193
2213	6	-21.895351318288995
2213	7	-10.679106161719094
2213	8	-10.447287711920119
2213	9	-4.260491546393034
2213	10-11	-17.693502455614592
2213	12-13	-16.29410939272497
2213	14-15	-23.746184531739363
2213	16-17	-18.06877805977886
2213	18-19	-18.51342615839805
2213	20-21	-17.8483323946614
2213	22-23	-19.90605404521709
2213	24-25	-7.49929047151349
2213	26-27	-23.428351393657014
2213	28-29	-24.344733035602758
2213	30-31	-19.642164245508045
2213	32-33	-23.535372704340464
2213	34-35	-18.066185808342755
2213	36-37	-23.997701163142303
2213	38-39	-11.634699457372175
2213	40-41	-17.312486322097804
2213	42-43	-23.532910589511562
2213	44-45	-17.3808663335966
2213	46-47	-17.21199643931821
2213	48-49	-6.176348566829869
2213	50-51	-23.7213916499206
2213	52-53	-18.14587311994593
2213	54-55	-18.046513270575133
2213	56-57	-17.859307972634358
2213	58-59	-18.408995495635104
2213	60-61	-19.615097630112757
2213	62-63	-17.84483344259825
2213	64-65	-17.91852416670386
2213	66-67	-8.862624591395633
2213	68-69	-23.128785027448743
2213	70-71	-17.81528287531303
2213	72-73	-9.598559108040561
2213	74-75	-4.530709015509025
2213	76-77	-3.308204504641683
2213	78-79	-4.6629027269648695
2213	80-81	-3.3934195091074315
2213	82-83	-3.6134253057602876
2213	84-85	-3.3507799781649155
2213	86-87	-3.2814211743719035
2213	88-89	-2.9114557469812787
2213	90-91	-2.9974837376955286
2213	92-93	-3.787607767915034
2213	94-95	-3.6611961788477956
2213	96-97	-3.5078553738291482
2213	98-99	-3.906684096646739
2213	100	-3.452539787939152
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	696.0
3	642.0
4	3252.0
5	11813.0
6	18266.0
7	20688.0
8	18594.0
9	18836.0
10	22504.0
11	28364.0
12	35334.0
13	48079.0
14	38537.0
15	42189.0
16	47614.0
17	50724.0
18	50746.0
19	51198.0
20	50922.0
21	49686.0
22	50786.0
23	55620.0
24	60013.0
25	65944.0
26	72654.0
27	77732.0
28	84233.0
29	88672.0
30	92671.0
31	100961.0
32	114311.0
33	138167.0
34	168532.0
35	200272.0
36	224123.0
37	203112.0
38	69920.0
39	196.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.52180335716305	21.274911590272435	14.392211017676695	23.81107403488782
2	14.991344058342914	21.29735093508177	40.6763638109617	23.034941195613612
3	17.62698807679938	27.103585891745226	29.733800923299814	25.535625108155585
4	12.064450280612474	17.971418597941806	39.573505166744326	30.390625954701395
5	13.096179569937853	37.60823573233319	36.21348792915846	13.082096768570489
6	29.73831677264791	38.4996661680014	18.836738300705864	12.92527875864482
7	25.463441813435452	31.86789781735055	25.104339189506558	17.56432117970744
8	23.319143937356447	36.51903600432262	22.637805620731974	17.52401443758896
9	23.98560612300176	17.616833499897684	22.70415029680154	35.69341008029902
10-11	22.901454701780402	27.752270801227247	29.487064930322	19.85920956667035
12-13	23.72560833946235	25.34897348101467	29.521278619675194	21.40413955984778
14-15	21.15709943082977	26.843336885113484	26.76824438569807	25.231319298358677
16-17	20.788878536727033	30.447817876708815	27.57969229987512	21.183611286689032
18-19	20.78720734088854	29.488512155496384	29.10690117111149	20.617379332503592
20-21	22.17213847417236	28.853074632783404	28.09714311846749	20.877643774576747
22-23	21.216203931952812	28.864464947056707	28.65624234672325	21.263088774267235
24-25	21.463354348539596	28.595300900897836	28.21748239119425	21.723862359368322
26-27	21.45329383869332	28.602301294824272	28.262369749819666	21.68203511666274
28-29	21.29138370347164	28.551544020473617	28.67795492757247	21.479117348482273
30-31	21.827093192999556	28.961694156051916	27.964939599510846	21.246273051437687
32-33	21.02888056753948	28.731233636247428	28.62020462983671	21.61968116637638
34-35	21.2272966375934	28.122829921391073	28.472212577428408	22.17766086358712
36-37	21.73593880154538	28.725207129626014	28.08278835577638	21.456065713052226
38-39	21.409975709322563	28.510908905880388	28.35473921551031	21.724376169286742
40-41	21.643279099481198	28.491948777586774	28.026529202645417	21.83824292028661
42-43	21.303327514105614	28.652398250569988	28.478101431185387	21.56617280413901
44-45	21.766682907269036	28.19761651194978	28.342811808786312	21.692888771994877
46-47	21.685914031294534	28.622099505275887	28.389541465387737	21.302444998041842
48-49	21.461413716645968	28.341241543493332	28.50487996139209	21.69246477846861
50-51	21.748684522332233	28.391978815219453	28.23631871959814	21.62301794285017
52-53	21.167327922267077	28.471275276245546	28.443961015330604	21.917435786156776
54-55	21.65402045645031	28.447011478541707	28.07888861692738	21.820079448080605
56-57	21.761847541973573	28.429063124872865	28.043537664931513	21.765551668222045
58-59	21.39019549244793	28.034808253660515	28.491373068033514	22.08362318585804
60-61	21.869071106953918	28.400851972331935	27.998891695415683	21.73118522529846
62-63	21.308654100970376	28.622866108738705	28.414127134047394	21.65435265624353
64-65	21.41761757906109	28.066951159044585	28.40949501758227	22.10593624431205
66-67	21.563926565053283	28.350995555009266	28.11098136812018	21.97409651181727
68-69	21.902827500734123	28.22827407310051	28.111366588197523	21.75753183796784
70-71	21.99318268971884	28.29506606946736	27.893419666890928	21.818331573922865
72-73	21.653488200041355	28.421426479608474	28.358961715944766	21.566123604405405
74-75	21.879141086189545	28.02142705226406	28.037240900677023	22.062190960869373
76-77	21.853864538153868	28.443547485925098	28.10553331204657	21.597054663874466
78-79	21.83004769723468	28.16993176839531	28.27570430835724	21.72431622601278
80-81	21.970525548264064	28.133835984334844	28.013823314956138	21.881815152444958
82-83	21.455343183486132	28.344941501912896	28.47641617146576	21.72329914313521
84-85	21.72523308629681	27.99095902742093	28.03606986681776	22.2477380194645
86-87	21.834938042644055	28.26194891864659	28.109448801011176	21.793664237698174
88-89	21.67811572395507	27.82714832031001	28.407513217569512	22.08722273816541
90-91	21.991156994642218	27.94619812958708	28.049671056163234	22.01297381960747
92-93	21.54726152060849	28.21659700195947	28.376685364576833	21.859456112855206
94-95	21.528942891286814	27.777063907867337	28.30097675673404	22.3930164441118
96-97	21.72922640843405	28.022915668221753	28.249023477740693	21.998834445603503
98-99	21.604758464091837	27.896623531946158	28.27178890289434	22.226829101067665
100	21.914792302282173	27.654537209719248	28.046472501255344	22.384197986743235
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	339.0
1	365.0
2	334.5
3	278.0
4	313.0
5	456.0
6	645.0
7	911.0
8	1336.5
9	1902.0
10	2647.0
11	3479.5
12	4308.0
13	5137.5
14	5963.5
15	6790.0
16	7472.0
17	8142.5
18	8978.5
19	9660.0
20	10305.5
21	11370.0
22	12741.5
23	14375.0
24	16611.0
25	19551.5
26	23325.5
27	28313.0
28	34367.0
29	41056.5
30	48202.0
31	55578.5
32	62767.5
33	69483.5
34	75768.0
35	81290.0
36	85932.5
37	90101.0
38	93323.0
39	95036.5
40	95485.0
41	94617.0
42	92517.0
43	89649.5
44	85838.5
45	81619.5
46	77435.5
47	73635.0
48	70771.5
49	68663.5
50	67326.5
51	63616.0
52	56902.5
53	51298.0
54	47548.5
55	43913.0
56	40137.0
57	36491.0
58	32864.5
59	29255.0
60	25864.5
61	22127.0
62	18211.5
63	15036.5
64	12308.0
65	9969.0
66	8033.0
67	6438.5
68	5039.5
69	3855.0
70	2904.0
71	2212.5
72	1715.5
73	1306.0
74	940.5
75	677.5
76	503.5
77	343.0
78	231.5
79	147.5
80	108.0
81	78.0
82	47.0
83	34.0
84	25.5
85	18.5
86	13.0
87	11.0
88	9.0
89	4.5
90	2.5
91	3.0
92	2.5
93	2.0
94	1.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	3.5
>>END_MODULE
>>Per base N content	fail
#Base	N-Count
1	7.721100232859284
2	24.314272412655562
3	5.967125130672942
4	24.003443426338418
5	11.977656491573336
6	9.527364700761487
7	7.674625283099472
8	8.569641561445254
9	4.892063847132544
10-11	6.685750602740932
12-13	9.799471291926885
14-15	7.27213848969738
16-17	7.819824170446374
18-19	6.501849509186575
20-21	7.734041346150351
22-23	5.610931586532035
24-25	5.087755284153334
26-27	9.541254694434272
28-29	6.171033468020511
30-31	4.21359418526102
32-33	8.555246844165174
34-35	6.966760518339031
36-37	6.518868789224595
38-39	5.9648639689122565
40-41	8.511578157661926
42-43	6.269696838774724
44-45	6.351966786763967
46-47	5.765033798311639
48-49	3.8659405645555625
50-51	6.370358915013831
52-53	4.355845486741314
54-55	4.732308730951226
56-57	4.6181200620365885
58-59	3.531329001862632
60-61	4.0377888583676915
62-63	3.0202458771147414
64-65	4.3962233753249915
66-67	2.4342819579884223
68-69	4.915987746118373
70-71	2.4447802090201782
72-73	1.4870166918153616
74-75	0.803338282316544
76-77	1.173886165848947
78-79	1.6821832162845638
80-81	2.093956924060901
82-83	0.8017433557174889
84-85	1.2277300802752804
86-87	0.45865243642198605
88-89	0.7398036746301285
90-91	0.33612573351481845
92-93	0.3468864408223684
94-95	0.5452024406010976
96-97	0.5409627622998116
98-99	0.47201751754318316
100	0.45001156826507926
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2476603.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.80152019043216
#Duplication Level	Percentage of deduplicated	Percentage of total
1	97.45861070690522	93.36683061369048
2	1.9887284979504303	3.8104642669937197
3	0.3031219954735762	0.871186439085777
4	0.0939618656318621	0.36006758269846034
5	0.05555869564075111	0.2661303751090746
6	0.028303534613015296	0.1626912985613628
7	0.018728053357056785	0.12559231872694965
8	0.012237039596895411	0.09378615968104749
9	0.00730501437658324	0.06298483340606727
>10	0.031492190644089466	0.4786428725940676
>50	6.78966907979124E-4	0.045083246297484045
>100	0.0011888108167886768	0.2470482155539972
>500	0.0	0.0
>1k	8.46280857645774E-5	0.10949177760154787
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	4.037788858367692E-5	0.0
8	0.0	0.0	0.0	4.037788858367692E-5	0.0
9	0.0	0.0	0.0	8.075577716735384E-5	0.0
10-11	0.0	0.0	0.0	8.075577716735384E-5	0.0
12-13	0.0	0.0	0.0	8.075577716735384E-5	0.0
14-15	2.018894429183846E-5	0.0	0.0	1.2113366575103074E-4	0.0
16-17	4.037788858367692E-5	0.0	0.0	1.413226100428692E-4	0.0
18-19	4.037788858367692E-5	0.0	0.0	1.6151155433470767E-4	0.0
20-21	4.037788858367692E-5	0.0	0.0	1.6151155433470767E-4	0.0
22-23	4.037788858367692E-5	0.0	0.0	2.0188944291838458E-4	0.0
24-25	4.037788858367692E-5	0.0	0.0	2.4226733150206148E-4	0.0
26-27	4.037788858367692E-5	0.0	0.0	3.0283416437757685E-4	0.0
28-29	4.037788858367692E-5	4.037788858367692E-5	0.0	6.056683287551537E-4	0.0
30-31	8.075577716735384E-5	4.037788858367692E-5	0.0	0.0019987054848920075	0.0
32-33	8.075577716735384E-5	4.037788858367692E-5	0.0	0.005289503404461676	0.0
34-35	8.075577716735384E-5	4.037788858367692E-5	0.0	0.010437684198880484	0.0
36-37	8.075577716735384E-5	4.037788858367692E-5	0.0	0.017887404642568876	0.0
38-39	8.075577716735384E-5	4.037788858367692E-5	0.0	0.030465116936384234	0.0
40-41	8.075577716735384E-5	4.037788858367692E-5	0.0	0.04695948442281625	0.0
42-43	1.0094472145919229E-4	4.037788858367692E-5	0.0	0.06448348806813203	0.0
44-45	1.2113366575103074E-4	4.037788858367692E-5	0.0	0.08594433585035632	0.0
46-47	1.2113366575103074E-4	4.037788858367692E-5	0.0	0.11095843782794416	0.0
48-49	1.2113366575103074E-4	4.037788858367692E-5	0.0	0.14067656382553037	0.0
50-51	1.2113366575103074E-4	4.037788858367692E-5	0.0	0.17158583753633505	0.0
52-53	1.2113366575103074E-4	4.037788858367692E-5	0.0	0.2032622911302296	0.0
54-55	1.2113366575103074E-4	4.037788858367692E-5	0.0	0.23950144613407964	0.0
56-57	1.2113366575103074E-4	4.037788858367692E-5	0.0	0.282948054250116	0.0
58-59	1.2113366575103074E-4	4.037788858367692E-5	0.0	0.33121982005190176	0.0
60-61	1.2113366575103074E-4	4.037788858367692E-5	0.0	0.3843571214280206	0.0
62-63	1.2113366575103074E-4	6.056683287551538E-5	0.0	0.4388268931274007	0.0
64-65	1.2113366575103074E-4	8.075577716735384E-5	0.0	0.5019779108722715	0.0
66-67	1.413226100428692E-4	8.075577716735384E-5	0.0	0.5763136037548207	0.0
68-69	2.0188944291838458E-4	8.075577716735384E-5	0.0	0.6662149726863773	0.0
70-71	2.0188944291838458E-4	8.075577716735384E-5	0.0	0.7699255795135515	0.0
72-73	2.0188944291838458E-4	8.075577716735384E-5	0.0	0.8757358365470768	0.0
74-75	2.0188944291838458E-4	1.0094472145919229E-4	0.0	0.9879863668096986	0.0
76-77	2.0188944291838458E-4	1.2113366575103074E-4	0.0	1.1118051621515437	0.0
78-79	2.4226733150206148E-4	1.2113366575103074E-4	0.0	1.2559138465066868	0.0
80-81	2.4226733150206148E-4	1.2113366575103074E-4	0.0	1.4208777103152987	0.0
82-83	2.4226733150206148E-4	1.2113366575103074E-4	0.0	1.6012053607299999	0.0
84-85	2.4226733150206148E-4	1.2113366575103074E-4	0.0	1.7863985467190342	0.0
86-87	2.4226733150206148E-4	1.2113366575103074E-4	0.0	1.9861479615424837	0.0
88	2.826452200857384E-4	1.2113366575103074E-4	0.0	2.1541199780505798	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGACCG	140	3.4686623E-4	21.396029	5
GTATAAG	975	0.0	19.063452	1
GTACATG	7550	0.0	18.463776	1
GTATAGA	1050	0.0	17.701778	1
TACATGG	7565	0.0	16.785213	2
GTATAGG	675	0.0	16.52166	1
CATGGGG	3375	0.0	15.605591	4
TATAGGA	1055	0.0	15.265212	2
ACATGGG	7775	0.0	15.205763	3
GTATTAG	990	0.0	15.0196905	1
AGGGTCG	235	6.4487744E-4	14.870998	5
GTATATA	1900	0.0	14.673843	1
GTATCAA	5365	0.0	13.857869	1
GTCCTGG	1480	0.0	13.814565	1
TACCGTC	220	0.007216369	13.593571	7
TTATACT	1290	0.0	13.464745	4
TACACCG	260	0.0013851718	13.441094	5
GTATTAA	1430	0.0	13.43107	1
GTAGTGC	455	3.1845047E-7	13.231271	6
GTATTAT	1405	0.0	13.229087	1
>>END_MODULE