FastQCFastQC Report
Wed 25 May 2016
SRR1295138_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295138_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2476603
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT41230.16647803463049993No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA29820.12040686375652455No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA76700.021.8187621
TCAACGC86250.019.2460524
CAACGCA86700.019.1461585
AACGCAG87350.019.084336
ATCAACG87300.018.9603213
TATCAAC86500.018.8746362
GAGTACT32100.018.4288412-13
ACGCAGA97050.017.1744527
CTAGTAC5550.016.9454963
CGCAGAG98300.016.8127678
AGTACTT38900.015.8711612-13
GTATAGG7000.015.6070281
CTATACT12450.015.4860474
GTACATG99500.015.4715631
GCAGAGT106000.015.2807889
CCTATAC7700.015.267453
CATGGGG39800.015.2416734
CCTACAC11450.015.1954473
TACATGG100250.014.8503152
TACACCG2257.3000736E-414.6299085
Produced by FastQC (version 0.11.5)