FastQCFastQC Report
Wed 25 May 2016
SRR1295137_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295137_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2485727
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT27560.11087299610938771No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACCG1451.5270247E-524.2256375
GTATTAT13450.016.1928271
GTCTAGC4804.397407E-815.5566811
GTATAGC5451.29566615E-814.8430711
GTCCGTC2050.00453107314.5821557
TACACTG15500.014.5688735
TTACACT14700.014.5629314
GTATATA21150.014.4165931
TATACTG14650.014.3865575
GTACATG114100.014.3432321
TACATGG114100.014.2844362
GTATTAG11000.014.1424361
CATGGGG30350.014.107094
ATACCGT2550.001152063113.7733766
GTATTAA12700.013.7192771
ACATGGG116600.013.5765793
GTACTAT7402.8376235E-1013.4544271
TGGACTG14450.013.1965675
CTGTGCG4603.5812445E-713.1041049
ATACTGT17700.013.0396856
Produced by FastQC (version 0.11.5)