##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295137_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2485727 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.810544762156102 27.0 22.0 33.0 6.0 33.0 2 21.951300766335162 27.0 2.0 33.0 2.0 33.0 3 24.24037675899244 27.0 15.0 33.0 15.0 33.0 4 24.338230626291622 33.0 6.0 37.0 2.0 37.0 5 26.09546864961438 33.0 15.0 37.0 2.0 37.0 6 26.97496949584568 33.0 15.0 37.0 2.0 37.0 7 26.696824309346923 33.0 15.0 37.0 6.0 37.0 8 27.550196783476224 33.0 15.0 37.0 6.0 37.0 9 28.57409924742339 33.0 22.0 37.0 15.0 37.0 10-11 28.143512541803666 33.0 18.5 37.0 6.0 37.0 12-13 26.985824670207144 33.0 15.0 37.0 4.0 37.0 14-15 28.611834485444298 33.0 18.5 37.0 6.0 40.0 16-17 29.01353064918231 33.0 22.0 37.0 6.0 40.0 18-19 29.192982978420396 33.0 22.0 37.0 6.0 40.0 20-21 28.687711884692085 33.0 18.5 37.0 6.0 40.0 22-23 29.412739814146928 33.0 22.0 37.0 6.0 40.0 24-25 29.578865458676677 33.0 24.5 37.0 6.0 40.0 26-27 28.15131146742985 33.0 15.0 37.0 4.0 40.0 28-29 28.86286627614376 33.0 22.0 37.0 6.0 40.0 30-31 29.5355302492993 33.0 22.0 37.0 6.0 40.0 32-33 28.361925303945284 33.0 22.0 37.0 6.0 40.0 34-35 27.954672415756036 33.0 15.0 37.0 6.0 40.0 36-37 28.237065252942095 33.0 22.0 37.0 6.0 40.0 38-39 28.358596297984455 33.0 22.0 37.0 6.0 40.0 40-41 27.376904221581853 33.0 15.0 37.0 6.0 40.0 42-43 27.991671048349236 33.0 18.5 37.0 6.0 40.0 44-45 27.85559154323866 33.0 22.0 37.0 6.0 40.0 46-47 27.58290612766406 33.0 15.0 37.0 6.0 40.0 48-49 27.492839318235674 33.0 18.5 37.0 6.0 40.0 50-51 27.44238204758608 33.0 22.0 37.0 6.0 40.0 52-53 27.64215358323742 33.0 22.0 37.0 6.0 40.0 54-55 27.61665259298386 33.0 22.0 37.0 6.0 40.0 56-57 27.57828635244337 33.0 22.0 37.0 6.0 40.0 58-59 27.480316824816242 33.0 22.0 37.0 6.0 40.0 60-61 27.457770302209376 33.0 22.0 37.0 6.0 40.0 62-63 27.428403440924928 33.0 22.0 37.0 6.0 40.0 64-65 26.97547095879797 33.0 22.0 37.0 6.0 40.0 66-67 27.15225445111229 33.0 22.0 37.0 6.0 37.0 68-69 26.764640485459587 30.0 18.5 37.0 6.0 37.0 70-71 26.886126272112747 27.0 22.0 37.0 6.0 37.0 72-73 26.933573759306633 27.0 22.0 37.0 6.0 37.0 74-75 27.034628299889732 27.0 22.0 37.0 6.0 37.0 76-77 26.792542584121264 27.0 22.0 35.0 6.0 37.0 78-79 26.408672191274427 27.0 22.0 33.0 6.0 37.0 80-81 26.189541932802758 27.0 22.0 33.0 6.0 37.0 82-83 26.311465217218142 27.0 22.0 33.0 6.0 37.0 84-85 26.26034415686035 27.0 22.0 33.0 6.0 37.0 86-87 26.040105771872774 27.0 22.0 33.0 6.0 37.0 88-89 26.007293842002763 27.0 22.0 33.0 6.0 37.0 90-91 26.1403036214355 27.0 22.0 33.0 6.0 37.0 92-93 26.049619889875274 27.0 22.0 33.0 6.0 37.0 94-95 25.930458372942805 27.0 22.0 33.0 6.0 37.0 96-97 26.010281096838067 27.0 22.0 33.0 6.0 37.0 98-99 26.069765304074018 27.0 22.0 33.0 10.5 37.0 100 25.740644889804873 27.0 22.0 33.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.282001788165225 1101 2 11.016980317957557 1101 3 8.252869154924216 1101 4 12.604637342944049 1101 5 10.684734755989567 1101 6 9.84995338448229 1101 7 9.386867142505391 1101 8 8.95895646497355 1101 9 7.206973387612656 1101 10-11 7.752016800175756 1101 12-13 9.048887131412801 1101 14-15 7.942352080967101 1101 16-17 8.02351003858736 1101 18-19 8.000873825060882 1101 20-21 7.1488137264973695 1101 22-23 7.226249426151558 1101 24-25 7.259568883977401 1101 26-27 8.318765178413347 1101 28-29 8.086478361055445 1101 30-31 5.503018746700167 1101 32-33 6.873806187254363 1101 34-35 8.686592642991581 1101 36-37 8.051013463883418 1101 38-39 7.9929966030945145 1101 40-41 8.947322467946908 1101 42-43 8.497321910883006 1101 44-45 8.35783102364272 1101 46-47 7.155068095917137 1101 48-49 5.433248100794497 1101 50-51 6.664177982626594 1101 52-53 5.602104115779248 1101 54-55 4.0065394794096285 1101 56-57 6.047223745591577 1101 58-59 6.005377106342657 1101 60-61 6.529764568903985 1101 62-63 5.937686987390528 1101 64-65 6.5075849546692055 1101 66-67 5.6145536474104745 1101 68-69 6.633640900759449 1101 70-71 6.118227075583373 1101 72-73 5.291265445581548 1101 74-75 4.662470875147118 1101 76-77 4.944848535242183 1101 78-79 5.023630067716862 1101 80-81 4.769359375684864 1101 82-83 4.347984955696276 1101 84-85 4.0883596156913775 1101 86-87 3.882156097843037 1101 88-89 3.8470591331377513 1101 90-91 3.666708415561839 1101 92-93 3.5713011314442333 1101 94-95 3.495694192718936 1101 96-97 3.671321710937729 1101 98-99 3.441503233895471 1101 100 3.6183840565475585 1102 1 6.6557370134019145 1102 2 10.27289108302979 1102 3 7.916744278298005 1102 4 12.429700878295154 1102 5 10.273687686525392 1102 6 9.384849681721128 1102 7 6.687013471554888 1102 8 7.619266042906236 1102 9 6.339721863280076 1102 10-11 7.347402878454059 1102 12-13 8.904320134717668 1102 14-15 6.154360004401408 1102 16-17 7.15224383664296 1102 18-19 7.271794666375545 1102 20-21 6.912887365602817 1102 22-23 6.499773583340936 1102 24-25 6.897289624185738 1102 26-27 8.315067919632742 1102 28-29 7.473384232444072 1102 30-31 4.148324352598724 1102 32-33 4.5041885514228355 1102 34-35 4.952346057049489 1102 36-37 5.230884966000108 1102 38-39 4.525416311261775 1102 40-41 6.117873101924825 1102 42-43 6.759325363629561 1102 44-45 6.426211052736587 1102 46-47 5.3255111754644275 1102 48-49 3.662237071951683 1102 50-51 4.967595823421963 1102 52-53 5.656807537230986 1102 54-55 5.209711776277054 1102 56-57 6.189245373403381 1102 58-59 5.890063797105185 1102 60-61 5.816827860086754 1102 62-63 4.254104126631816 1102 64-65 6.0983189384302925 1102 66-67 3.8855333814389468 1102 68-69 6.058230371258073 1102 70-71 5.141137115028595 1102 72-73 5.014203340850528 1102 74-75 4.161115998830418 1102 76-77 3.1774153462318218 1102 78-79 4.273078969475218 1102 80-81 4.383876816525628 1102 82-83 3.8432057045020898 1102 84-85 3.06540796446005 1102 86-87 3.1030226031064174 1102 88-89 3.246855458920205 1102 90-91 3.164288827759247 1102 92-93 3.060177716389024 1102 94-95 3.041218862317912 1102 96-97 3.3058169358992977 1102 98-99 3.1391306742891665 1102 100 3.0820081336084577 1103 1 6.553461279335867 1103 2 10.724466614833695 1103 3 7.717651345549509 1103 4 11.804958615676998 1103 5 10.024294867489083 1103 6 9.062748925890197 1103 7 7.999233278866662 1103 8 7.897360272341825 1103 9 7.071680597243542 1103 10-11 7.34261241309331 1103 12-13 8.696340467893481 1103 14-15 8.450927685067445 1103 16-17 7.964188963720929 1103 18-19 7.203333357887633 1103 20-21 8.229295323175993 1103 22-23 6.826148496873213 1103 24-25 5.2958779098146 1103 26-27 5.935919857956382 1103 28-29 6.9015771381015405 1103 30-31 6.135728164104787 1103 32-33 7.94055602149777 1103 34-35 7.31013849768814 1103 36-37 4.1712601548263315 1103 38-39 6.363723814599933 1103 40-41 6.772689562398664 1103 42-43 7.523108603839308 1103 44-45 5.72027925725018 1103 46-47 6.2579838499100475 1103 48-49 5.5017599016227585 1103 50-51 6.316672142093122 1103 52-53 6.022760251550391 1103 54-55 4.826267638591503 1103 56-57 5.878411066166571 1103 58-59 5.4410262300022865 1103 60-61 5.012232467665754 1103 62-63 2.8798342035475386 1103 64-65 4.958812942019634 1103 66-67 4.087262648254011 1103 68-69 5.117004839761027 1103 70-71 3.626516814642571 1103 72-73 4.274482720369537 1103 74-75 3.2813771072480122 1103 76-77 3.6151151864035214 1103 78-79 2.9313211737906677 1103 80-81 4.055498218964171 1103 82-83 3.4890958025996675 1103 84-85 3.3906199802517527 1103 86-87 2.6942560690308994 1103 88-89 2.906522966957649 1103 90-91 2.673531252134513 1103 92-93 2.404650664375122 1103 94-95 2.8762643954106153 1103 96-97 2.9322454177759276 1103 98-99 2.7616297296100996 1103 100 2.917968536870255 1104 1 6.107800566811008 1104 2 9.27606622212953 1104 3 7.043995561980179 1104 4 11.714432315798138 1104 5 9.135651618639447 1104 6 7.949775940856288 1104 7 3.869157365178083 1104 8 6.394842567904973 1104 9 6.462661465151427 1104 10-11 6.885837456477187 1104 12-13 7.243212887983294 1104 14-15 7.014636360619381 1104 16-17 6.789049131239196 1104 18-19 6.592223342419874 1104 20-21 7.11073371734992 1104 22-23 6.1987637331614955 1104 24-25 5.720074831232743 1104 26-27 7.92199960727638 1104 28-29 6.946214397780775 1104 30-31 6.169908217813703 1104 32-33 7.507285931577442 1104 34-35 7.833435783955505 1104 36-37 6.506033763321295 1104 38-39 6.954858928297728 1104 40-41 6.853786949577188 1104 42-43 6.960088888017207 1104 44-45 6.3208045242652595 1104 46-47 6.394951268276021 1104 48-49 5.202375733157279 1104 50-51 5.506586515166397 1104 52-53 5.71308832795496 1104 54-55 4.543981990215766 1104 56-57 5.769019455323733 1104 58-59 3.6716898503805773 1104 60-61 4.078003613842252 1104 62-63 3.6501981746676613 1104 64-65 2.744688529798303 1104 66-67 3.0681889831134903 1104 68-69 3.935515846058255 1104 70-71 2.3346897361931482 1104 72-73 2.2185672719663003 1104 74-75 2.9375017195642137 1104 76-77 3.5920225655134708 1104 78-79 4.11871535923542 1104 80-81 2.909787230805037 1104 82-83 2.803672821488476 1104 84-85 2.766296470040796 1104 86-87 0.6694002021126977 1104 88-89 0.1633462558406542 1104 90-91 2.09200274329471 1104 92-93 2.3863911802024127 1104 94-95 2.3314946088715267 1104 96-97 2.7401150660480234 1104 98-99 2.311509402778892 1104 100 0.8461119876141687 1105 1 5.857186411552963 1105 2 -2.5981631406481185 1105 3 0.6042605412265623 1105 4 8.236897315176748 1105 5 8.52878579559652 1105 6 8.14989313402095 1105 7 5.353564611569297 1105 8 5.971419941386973 1105 9 4.962800180211467 1105 10-11 5.012699363240795 1105 12-13 5.233161014383139 1105 14-15 5.908639603808442 1105 16-17 6.016819007814831 1105 18-19 6.177635673565877 1105 20-21 7.195145341563947 1105 22-23 2.348712030930063 1105 24-25 4.265514093909456 1105 26-27 6.973600043532183 1105 28-29 6.029439023581322 1105 30-31 5.543346620334635 1105 32-33 7.335874007396143 1105 34-35 6.451716185954847 1105 36-37 6.464974537570942 1105 38-39 4.374889030149788 1105 40-41 5.671188266034591 1105 42-43 5.745910765207466 1105 44-45 6.278070907295092 1105 46-47 5.426297163656709 1105 48-49 5.0705537279445 1105 50-51 6.064191769043401 1105 52-53 5.64062463184268 1105 54-55 5.3783875121192075 1105 56-57 5.373275541541496 1105 58-59 2.229894390758865 1105 60-61 4.4705448766223626 1105 62-63 -0.5993787317985699 1105 64-65 0.008243021667720285 1105 66-67 1.1655256071583082 1105 68-69 2.9992884838304263 1105 70-71 3.069133506443279 1105 72-73 2.782217701518565 1105 74-75 2.827778017066649 1105 76-77 1.957620434982637 1105 78-79 3.421627738263865 1105 80-81 1.781331072900425 1105 82-83 1.194341052938789 1105 84-85 1.6252690483091392 1105 86-87 -0.08984731854911487 1105 88-89 1.768488833177269 1105 90-91 1.5510060167253137 1105 92-93 1.7591629727955684 1105 94-95 1.1247795038995463 1105 96-97 2.1715361296544735 1105 98-99 2.144627715168518 1105 100 2.249449263868179 1106 1 5.895346477406914 1106 2 10.067981795768628 1106 3 6.707126998757104 1106 4 11.56332333469069 1106 5 9.655696493878807 1106 6 9.248114342219935 1106 7 6.627147373897856 1106 8 7.267847581594886 1106 9 6.830388989677303 1106 10-11 5.671064845271442 1106 12-13 7.781781710077741 1106 14-15 7.710953051942166 1106 16-17 7.479335094497536 1106 18-19 6.968997720070664 1106 20-21 8.048281483064091 1106 22-23 6.0662831657044585 1106 24-25 4.904040875692267 1106 26-27 7.444339116983667 1106 28-29 6.225057278861097 1106 30-31 5.381131805119647 1106 32-33 7.354108171279634 1106 34-35 6.609215571102535 1106 36-37 5.815229692031462 1106 38-39 6.791420823996528 1106 40-41 6.554395804407665 1106 42-43 6.135686307726754 1106 44-45 4.362085758476159 1106 46-47 5.88301057390024 1106 48-49 5.707897181117914 1106 50-51 6.46263111722347 1106 52-53 5.4483781353908185 1106 54-55 5.149733741440247 1106 56-57 4.938269809909176 1106 58-59 4.98786483788539 1106 60-61 4.766739156633385 1106 62-63 4.854512771497458 1106 64-65 5.049791003953068 1106 66-67 3.081731042082758 1106 68-69 3.3321426393810327 1106 70-71 2.9740000039361334 1106 72-73 2.8309801154299876 1106 74-75 2.8070531154368545 1106 76-77 3.39034547771395 1106 78-79 3.984029636325918 1106 80-81 3.626730312481669 1106 82-83 2.0185688983359285 1106 84-85 2.626254415440723 1106 86-87 3.2365209776180315 1106 88-89 3.318595535030507 1106 90-91 3.092403276938459 1106 92-93 2.842619091581337 1106 94-95 1.3589099621168437 1106 96-97 1.5280944762407422 1106 98-99 1.95835546176993 1106 100 2.7277340614874106 1107 1 5.899868088429436 1107 2 10.158803290945595 1107 3 5.99338194184967 1107 4 11.18344387798825 1107 5 9.206115649179164 1107 6 7.874205343769258 1107 7 3.495804751896589 1107 8 6.100636462966886 1107 9 5.583656479216984 1107 10-11 4.508045509443207 1107 12-13 5.996701467373381 1107 14-15 5.94442702996432 1107 16-17 6.795131662092118 1107 18-19 6.167777831147777 1107 20-21 5.546625554124709 1107 22-23 5.5975062265216735 1107 24-25 4.464624499299418 1107 26-27 6.9980612564949745 1107 28-29 6.202773678275989 1107 30-31 5.02926671216321 1107 32-33 4.850078748318346 1107 34-35 6.56040689812809 1107 36-37 6.390584720056697 1107 38-39 6.3009495523369985 1107 40-41 7.473477764431898 1107 42-43 5.9818708568256405 1107 44-45 4.017285837412917 1107 46-47 3.5409544015324563 1107 48-49 3.9361000060129463 1107 50-51 4.725647197016624 1107 52-53 3.9340664824195315 1107 54-55 5.154850047296275 1107 56-57 5.273670148084303 1107 58-59 3.1878515910195233 1107 60-61 2.860019736070459 1107 62-63 0.5455150773783757 1107 64-65 1.9959936680614767 1107 66-67 4.056712318997192 1107 68-69 4.988076121659997 1107 70-71 4.299791662377277 1107 72-73 2.4996359626326417 1107 74-75 2.5435119867289337 1107 76-77 2.9935023964031515 1107 78-79 3.8673556114677687 1107 80-81 2.0926629759791773 1107 82-83 1.5908133522581878 1107 84-85 2.278376728098152 1107 86-87 2.2883946013156873 1107 88-89 2.549731831881356 1107 90-91 1.3978463070011742 1107 92-93 2.3004639194845034 1107 94-95 2.3667623803645554 1107 96-97 2.306979131144864 1107 98-99 1.873742422979209 1107 100 0.21064083183434335 1108 1 4.455916955286963 1108 2 -3.249531820503398 1108 3 0.11197878250318638 1108 4 6.67767056017987 1108 5 7.066127511918996 1108 6 6.923374245444876 1108 7 4.648730835510889 1108 8 5.038017835568549 1108 9 4.987531829305581 1108 10-11 3.593299131146864 1108 12-13 2.7859731241155714 1108 14-15 4.112882528086345 1108 16-17 4.648220513942817 1108 18-19 4.109035313364263 1108 20-21 4.879076947978977 1108 22-23 4.316195111155647 1108 24-25 3.139066742099107 1108 26-27 4.731350655063249 1108 28-29 6.127591550952541 1108 30-31 4.604455410271665 1108 32-33 4.735061749061622 1108 34-35 3.254566212109875 1108 36-37 1.5294327024724303 1108 38-39 2.68639699453529 1108 40-41 5.575336113144164 1108 42-43 3.128399857303261 1108 44-45 3.8139732609303927 1108 46-47 4.835115835122309 1108 48-49 4.812793767158549 1108 50-51 2.3801751037764802 1108 52-53 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-5.173688079379396 2212 98-99 -3.3869652244822 2212 100 -3.4098800911866682 2213 1 -20.79235245537721 2213 2 -16.7095879477251 2213 3 -19.559676970537037 2213 4 -18.640196605395435 2213 5 -20.655855649914493 2213 6 -21.435839973451287 2213 7 -10.313338907000361 2213 8 -10.065751519332569 2213 9 -4.334206885503946 2213 10-11 -17.305414449319223 2213 12-13 -15.969874371895664 2213 14-15 -23.21078445039822 2213 16-17 -17.608228767851795 2213 18-19 -18.26689962822109 2213 20-21 -17.395925726371612 2213 22-23 -19.485290917354067 2213 24-25 -7.776100125997367 2213 26-27 -22.911493124169677 2213 28-29 -23.763521638944557 2213 30-31 -19.138588888354285 2213 32-33 -22.971673517542683 2213 34-35 -17.49391009086787 2213 36-37 -23.33099760622729 2213 38-39 -11.225381755871553 2213 40-41 -16.743861171991682 2213 42-43 -22.850833070666805 2213 44-45 -16.79910283401702 2213 46-47 -16.62606647820233 2213 48-49 -6.150012958093466 2213 50-51 -22.969216851321743 2213 52-53 -17.433722685919424 2213 54-55 -17.355723833004518 2213 56-57 -17.130405966836936 2213 58-59 -17.616804863652227 2213 60-61 -18.934949565463636 2213 62-63 -17.262535896225174 2213 64-65 -17.249517244090008 2213 66-67 -8.707095466890884 2213 68-69 -22.419495630605862 2213 70-71 -17.16051570819483 2213 72-73 -9.469358448978566 2213 74-75 -4.519014521823639 2213 76-77 -3.325990072778932 2213 78-79 -4.707848371155119 2213 80-81 -3.5506218150029554 2213 82-83 -3.355009046871487 2213 84-85 -3.1329748129813737 2213 86-87 -3.2813235351284717 2213 88-89 -2.8135865335378973 2213 90-91 -2.900103024450374 2213 92-93 -3.7732333941929923 2213 94-95 -3.769449772589333 2213 96-97 -3.3816085464670316 2213 98-99 -3.868625962287002 2213 100 -3.445984192974354 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 744.0 3 685.0 4 3669.0 5 11822.0 6 17954.0 7 22721.0 8 21223.0 9 22345.0 10 26499.0 11 32943.0 12 41823.0 13 55860.0 14 48859.0 15 52192.0 16 57057.0 17 58621.0 18 58018.0 19 56421.0 20 54833.0 21 53429.0 22 55231.0 23 59197.0 24 63155.0 25 69008.0 26 75088.0 27 80125.0 28 85908.0 29 89544.0 30 93198.0 31 100870.0 32 113543.0 33 136109.0 34 162623.0 35 188243.0 36 200209.0 37 167121.0 38 48740.0 39 97.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.61775385713129 21.19208283995176 13.939253328547258 24.250909974369694 2 14.498850178870004 22.012785088319244 40.3610014574593 23.12736327535145 3 18.13039839066662 27.435933627439667 29.534219141680286 24.899448840213427 4 11.912674847350106 18.258155820161114 39.267440060884454 30.561729271604328 5 12.78683688989261 38.13462690694566 35.596626261695164 13.481909941466563 6 29.485125448028676 38.49905913978495 19.287186379928315 12.728629032258066 7 25.391444543933954 31.958408716331483 24.326848426125537 18.323298313609026 8 24.193873659978706 36.491334829853734 21.96186250740848 17.352929002759083 9 24.296496209299452 17.50486341984616 22.52020926540012 35.678431105454266 10-11 22.853480845669562 27.95619334988228 29.23526002551302 19.955065778935133 12-13 23.768565954653003 25.4886614592655 29.324839554446207 21.417933031635286 14-15 21.296289639995546 26.989779480144847 26.773135044312447 24.940795835547156 16-17 20.75609054828829 30.664289774828372 27.62112717538337 20.95849250149996 18-19 20.762413342319537 29.628854407352566 29.174933480072635 20.43379877025526 20-21 22.06224648643908 28.930189217040496 28.22502137735973 20.782542919160694 22-23 21.040957147384397 29.14077375079761 28.60237194057848 21.21589716123951 24-25 21.31976396192567 28.947926255731137 28.12355168398663 21.60875809835656 26-27 21.329337070102817 28.741268589643298 28.33600303893366 21.593391301320228 28-29 21.126819560908086 28.686852934024508 28.84836970951469 21.337957795552715 30-31 21.851164742987716 29.06507646839378 28.133504987489744 20.950253801128763 32-33 20.670149049516926 28.980376838843796 28.88996288106493 21.45951123057435 34-35 20.759650477587567 28.620148926299088 28.66897472977013 21.951225866343215 36-37 21.57451300068023 29.12390874645519 28.10440626522431 21.19717198764027 38-39 21.443725213530538 28.727037038545806 28.357854388222176 21.47138335970148 40-41 21.410589749531002 28.80403390419787 28.042331431580525 21.743044914690604 42-43 21.236457049654113 28.873944745709874 28.5524920923207 21.33710611231531 44-45 21.73180321742803 28.344140035224925 28.47430694247901 21.44974980486803 46-47 21.43905876218937 28.85181936388239 28.676149275769557 21.03297259815868 48-49 21.36713686873999 28.59801808589859 28.685832628959147 21.349012416402275 50-51 21.526835596507198 28.637511455176924 28.467455664641538 21.36819728367434 52-53 20.92331711315205 28.752135209823432 28.50899059569587 21.81555708132865 54-55 21.431843424145487 28.761939832692967 28.159762493680752 21.646454249480794 56-57 21.666742120982747 28.593723357398325 28.11222609554378 21.62730842607515 58-59 21.254302820607684 28.153697846375103 28.719611899389815 21.872387433627395 60-61 21.793972019235202 28.66852394548069 28.113331740182208 21.424172295101897 62-63 21.116396122236814 28.847191544627133 28.554814916929505 21.481597416206547 64-65 21.061085245683138 28.34926594955834 28.662406814863058 21.92724198989547 66-67 21.365566119181047 28.556156168327167 28.283430042305742 21.794847670186044 68-69 21.903406783280836 28.377170927487537 28.229241713790167 21.49018057544146 70-71 21.810855550197186 28.58292947640935 27.968619465060396 21.637595508333067 72-73 21.558618739097525 28.658470293768072 28.432246478101863 21.35066448903254 74-75 21.868702591229603 28.187876423962717 28.10055235567988 21.8428686291278 76-77 21.679718446852736 28.680003002170473 28.263299299920696 21.3769792510561 78-79 21.738371064506858 28.37340619494093 28.513273485648728 21.374949254903484 80-81 21.803394025421845 28.350161294837743 28.236909100190523 21.609535579549892 82-83 21.186407183334875 28.66799371593998 28.508216767235815 21.637382333489334 84-85 21.474980495572247 28.292930553245917 28.10929481392888 22.122794137252956 86-87 21.731174153541957 28.48501395547546 28.119590876508138 21.664221014474446 88-89 21.538218341541178 28.06127354193287 28.555646795054606 21.844861321471342 90-91 21.905739485290603 28.187724374241668 28.176032572553172 21.730503567914553 92-93 21.352610750938897 28.51367632272506 28.507906573640557 21.62580635269548 94-95 21.244141545084744 28.076711550726014 28.505237642252922 22.17390926193632 96-97 21.587805730944027 28.251376652594917 28.388460448655163 21.772357167805897 98-99 21.628672803027975 28.030582911209233 28.3939626418151 21.946781643947684 100 21.65496561137156 27.97042703099195 28.222210649676015 22.152396707960474 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 286.0 1 351.5 2 328.5 3 243.5 4 296.0 5 414.0 6 613.0 7 929.5 8 1374.5 9 2038.0 10 2795.0 11 3590.5 12 4395.5 13 5182.5 14 6051.0 15 6936.5 16 7696.0 17 8457.5 18 9345.5 19 10191.5 20 11246.0 21 12299.5 22 13514.5 23 15484.0 24 17833.0 25 20657.5 26 24707.0 27 29773.0 28 35882.5 29 42812.0 30 49900.5 31 57268.0 32 64781.0 33 71648.0 34 77589.5 35 83449.0 36 88550.5 37 92051.5 38 94313.0 39 96159.0 40 96304.0 41 94865.0 42 92612.5 43 88894.5 44 84932.0 45 80904.0 46 77051.0 47 73898.5 48 71319.0 49 70127.5 50 70320.5 51 66419.5 52 57857.5 53 50603.5 54 45506.0 55 41278.0 56 37674.5 57 34156.0 58 30381.5 59 26879.5 60 23349.0 61 19532.0 62 16228.5 63 13333.5 64 10732.5 65 8676.0 66 6948.5 67 5489.0 68 4301.0 69 3340.0 70 2613.5 71 1999.0 72 1511.5 73 1164.5 74 885.5 75 682.0 76 500.0 77 352.0 78 243.5 79 164.5 80 119.5 81 83.5 82 55.0 83 39.5 84 27.5 85 22.5 86 18.0 87 12.5 88 7.0 89 2.0 90 2.5 91 4.0 92 5.0 93 3.0 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 1.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 8.09911949300949 2 25.00371118791404 3 6.2285198656167795 4 24.35681794501166 5 12.713664855392407 6 10.207355835938541 7 8.075263293193501 8 8.636547778577453 9 5.493644314118164 10-11 6.95130639849026 12-13 10.201582876961146 14-15 7.663411951513581 16-17 8.072889742115686 18-19 6.494015634057964 20-21 8.25822787458156 22-23 5.807274893823819 24-25 5.298248761831046 26-27 9.8750787998843 28-29 6.3605134433507775 30-31 4.347943277761395 32-33 8.852017940827773 34-35 7.347608969126537 36-37 7.05960469512541 38-39 6.182637916392267 40-41 8.935554869863022 42-43 6.569546856915502 44-45 6.555023942693626 46-47 5.8666740152880825 48-49 4.0007611455320715 50-51 6.62679369053802 52-53 4.581657599567451 54-55 5.0638706503167885 56-57 4.918721967456603 58-59 3.494530976249604 60-61 4.043766672687708 62-63 2.967723326012873 64-65 4.622752216957051 66-67 2.724896981848771 68-69 5.156660405587581 70-71 2.606863102826658 72-73 1.5703655308889513 74-75 0.8816736512094852 76-77 1.1065374435728461 78-79 1.6457760647086346 80-81 2.2784078863044894 82-83 0.7583495693613982 84-85 1.1760543293772807 86-87 0.467006231979618 88-89 0.6206433771689329 90-91 0.215490277089962 92-93 0.29299275423246396 94-95 0.5537816501973065 96-97 0.543764460055348 98-99 0.47193436769202735 100 0.34597524185077444 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2485727.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 96.97225201758619 #Duplication Level Percentage of deduplicated Percentage of total 1 98.34411465497334 95.36650270768465 2 1.3711830167014958 2.659334101156231 3 0.1618954640069255 0.470981032085509 4 0.050272569004567356 0.1950017692432959 5 0.02551013054768334 0.12368874042357364 6 0.013956230092304081 0.08120202370357989 7 0.006431235130074984 0.04365559476725896 8 0.005150904298808772 0.03995958318260419 9 0.003735390953234788 0.03260063456111658 >10 0.0152287293666386 0.2517605398472937 >50 9.343617856632339E-4 0.06354437157337532 >100 0.0012118400758060973 0.25341261825288025 >500 2.5031537566716944E-4 0.17392067139070855 >1k 1.2515768783286538E-4 0.24443561212784543 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 2756 0.11087299610938771 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 4.0229679284973774E-5 0.0 0.0 0.0 0.0 8 4.0229679284973774E-5 0.0 0.0 0.0 0.0 9 4.0229679284973774E-5 0.0 0.0 4.0229679284973774E-5 0.0 10-11 4.0229679284973774E-5 0.0 0.0 4.0229679284973774E-5 0.0 12-13 6.034451892746066E-5 4.0229679284973774E-5 0.0 6.034451892746066E-5 0.0 14-15 8.045935856994755E-5 4.0229679284973774E-5 0.0 8.045935856994755E-5 0.0 16-17 8.045935856994755E-5 4.0229679284973774E-5 0.0 8.045935856994755E-5 0.0 18-19 8.045935856994755E-5 4.0229679284973774E-5 0.0 1.2068903785492131E-4 0.0 20-21 8.045935856994755E-5 4.0229679284973774E-5 0.0 2.0114839642486885E-4 0.0 22-23 8.045935856994755E-5 4.0229679284973774E-5 0.0 2.0114839642486885E-4 0.0 24-25 8.045935856994755E-5 4.0229679284973774E-5 0.0 2.614929153523295E-4 0.0 26-27 1.2068903785492131E-4 4.0229679284973774E-5 0.0 3.620671135647639E-4 0.0 28-29 1.609187171398951E-4 4.0229679284973774E-5 0.0 6.034451892746066E-4 0.0 30-31 1.609187171398951E-4 4.0229679284973774E-5 0.0 0.0017097613696113855 0.0 32-33 1.609187171398951E-4 4.0229679284973774E-5 0.0 0.004284460843849707 0.0 34-35 1.609187171398951E-4 4.0229679284973774E-5 0.0 0.008488462329129465 0.0 36-37 1.609187171398951E-4 4.0229679284973774E-5 0.0 0.015206818769720087 4.0229679284973774E-5 38-39 1.609187171398951E-4 4.0229679284973774E-5 0.0 0.02733606707413968 4.0229679284973774E-5 40-41 1.609187171398951E-4 4.0229679284973774E-5 0.0 0.04300552715563696 4.0229679284973774E-5 42-43 1.609187171398951E-4 4.0229679284973774E-5 0.0 0.058212345925357054 4.0229679284973774E-5 44-45 1.609187171398951E-4 4.0229679284973774E-5 0.0 0.07502835186647608 4.0229679284973774E-5 46-47 1.609187171398951E-4 4.0229679284973774E-5 0.0 0.09713456063356918 4.0229679284973774E-5 48-49 1.8103355678238196E-4 4.0229679284973774E-5 0.0 0.12115167916669853 4.0229679284973774E-5 50-51 2.0114839642486885E-4 6.034451892746066E-5 0.0 0.1481860236462009 4.0229679284973774E-5 52-53 2.0114839642486885E-4 8.045935856994755E-5 0.0 0.17819736439279132 4.0229679284973774E-5 54-55 2.0114839642486885E-4 8.045935856994755E-5 0.0 0.21221155822823665 4.0229679284973774E-5 56-57 2.0114839642486885E-4 1.0057419821243442E-4 0.0 0.25119411745537623 4.0229679284973774E-5 58-59 2.0114839642486885E-4 1.2068903785492131E-4 0.0 0.29369677361995106 4.0229679284973774E-5 60-61 2.0114839642486885E-4 1.2068903785492131E-4 0.0 0.338271258267702 6.034451892746066E-5 62-63 2.0114839642486885E-4 1.2068903785492131E-4 0.0 0.38781410830714713 8.045935856994755E-5 64-65 2.0114839642486885E-4 1.2068903785492131E-4 0.0 0.4429891134464887 8.045935856994755E-5 66-67 2.0114839642486885E-4 1.2068903785492131E-4 0.0 0.510796237881312 8.045935856994755E-5 68-69 2.0114839642486885E-4 1.2068903785492131E-4 0.0 0.5871521691641922 8.045935856994755E-5 70-71 2.0114839642486885E-4 1.408038774974082E-4 0.0 0.679479283123207 8.045935856994755E-5 72-73 2.0114839642486885E-4 1.609187171398951E-4 0.0 0.7757489056521493 8.045935856994755E-5 74-75 2.0114839642486885E-4 1.609187171398951E-4 0.0 0.8766047116195785 8.045935856994755E-5 76-77 2.0114839642486885E-4 1.609187171398951E-4 0.0 0.9886845981075154 8.045935856994755E-5 78-79 2.0114839642486885E-4 1.609187171398951E-4 0.0 1.115910958846245 8.045935856994755E-5 80-81 2.0114839642486885E-4 2.0114839642486885E-4 0.0 1.261743546254275 8.045935856994755E-5 82-83 2.0114839642486885E-4 2.0114839642486885E-4 0.0 1.420811698147061 8.045935856994755E-5 84-85 2.2126323606735574E-4 2.0114839642486885E-4 0.0 1.5834602914962101 8.045935856994755E-5 86-87 2.4137807570984263E-4 2.0114839642486885E-4 0.0 1.7610944403790119 8.045935856994755E-5 88 2.4137807570984263E-4 2.0114839642486885E-4 0.0 1.9102660911676945 8.045935856994755E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTACCG 145 1.5270247E-5 24.225637 5 GTATTAT 1345 0.0 16.192827 1 GTCTAGC 480 4.397407E-8 15.556681 1 GTATAGC 545 1.29566615E-8 14.843071 1 GTCCGTC 205 0.004531073 14.582155 7 TACACTG 1550 0.0 14.568873 5 TTACACT 1470 0.0 14.562931 4 GTATATA 2115 0.0 14.416593 1 TATACTG 1465 0.0 14.386557 5 GTACATG 11410 0.0 14.343232 1 TACATGG 11410 0.0 14.284436 2 GTATTAG 1100 0.0 14.142436 1 CATGGGG 3035 0.0 14.10709 4 ATACCGT 255 0.0011520631 13.773376 6 GTATTAA 1270 0.0 13.719277 1 ACATGGG 11660 0.0 13.576579 3 GTACTAT 740 2.8376235E-10 13.454427 1 TGGACTG 1445 0.0 13.196567 5 CTGTGCG 460 3.5812445E-7 13.104104 9 ATACTGT 1770 0.0 13.039685 6 >>END_MODULE