FastQCFastQC Report
Wed 25 May 2016
SRR1295137_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295137_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2485727
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT81500.32787188617253626No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA56860.22874595641436085No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGG7350.018.2764421
TGTAGCG1851.6472948E-417.7885195
GACCGTG3501.5334081E-917.438957
GTATAGG8050.017.3052311
GTACCGT1650.001580873617.0765346
GAGTACT31900.016.85198412-13
AGTACTT38800.016.21471612-13
ACTACGC1850.003368769315.2469953
CCTACAC9900.015.1956583
TATACTG15600.015.0681145
CTATAGG9650.014.9515391
CTACACT12950.014.8842714
AACTGCG2601.3441412E-414.4464667
GTATCAA118800.014.364581
TATACAG14750.014.34285
ATATACT19800.014.008784
CATGGGT20950.013.9130124
CTAGTAC6157.2759576E-1213.7594833
CTATACA12800.013.5895554
CCTATAC8750.013.4318763
Produced by FastQC (version 0.11.5)