FastQCFastQC Report
Wed 25 May 2016
SRR1295136_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295136_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3717410
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG9700.018.9243411
GTATTAG14550.016.5316091
GTGTTAG16600.015.2526881
ACACCGT4701.6534614E-915.1228866
GTACATG127800.015.0074271
TATACTG18250.014.9765025
GTATAAG13200.014.8655931
TACATGG129900.014.7623682
GTATATA21550.014.6864641
GTACCGT2457.788129E-414.5056276
GTATTAT19450.014.31948951
ACATGGG132800.014.2116023
TAGACCG2200.006519241713.8040995
GTATAGA15400.013.5639981
TCTGTCG7251.8189894E-1213.3140058
CATGGGG47850.013.2668634
ACAGCGC5401.1557859E-813.1712638
GTATAAA23850.013.0047551
GTCTTGC19600.012.595141
CTGTCGC8251.8189894E-1212.319679
Produced by FastQC (version 0.11.5)