##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295136_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3717410 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.788661998541997 27.0 22.0 33.0 6.0 33.0 2 21.721252431128125 27.0 2.0 33.0 2.0 33.0 3 24.294060380748963 27.0 15.0 33.0 6.0 33.0 4 23.995026644895237 33.0 2.0 37.0 2.0 37.0 5 26.227364213256003 33.0 15.0 37.0 2.0 37.0 6 27.196752039726583 33.0 15.0 37.0 2.0 37.0 7 26.983854619210685 33.0 15.0 37.0 6.0 37.0 8 27.6644098982894 33.0 15.0 37.0 2.0 37.0 9 28.782573350800693 33.0 22.0 37.0 6.0 37.0 10-11 28.305761672777553 33.0 18.5 37.0 4.0 37.0 12-13 27.086749242079833 33.0 15.0 37.0 4.0 37.0 14-15 28.879795475882403 33.0 22.0 37.0 4.0 40.0 16-17 29.274521239249907 33.0 24.5 38.5 4.0 40.0 18-19 29.490584170161483 33.0 22.0 40.0 6.0 40.0 20-21 28.972531547502157 33.0 22.0 40.0 4.0 40.0 22-23 29.793647727853532 33.0 27.0 40.0 6.0 40.0 24-25 30.022435916404163 33.0 27.0 40.0 10.5 40.0 26-27 28.42207840943022 33.0 18.5 38.5 2.0 40.0 28-29 29.18099106636072 33.0 22.0 40.0 6.0 40.0 30-31 29.958660465216376 33.0 27.0 40.0 10.5 40.0 32-33 28.70473743816259 33.0 22.0 37.0 4.0 40.0 34-35 28.320694246800862 33.0 22.0 37.0 6.0 40.0 36-37 28.630971832539323 33.0 22.0 37.0 6.0 40.0 38-39 28.78844141485604 33.0 22.0 37.0 6.0 40.0 40-41 27.76000333565574 33.0 15.0 37.0 4.0 40.0 42-43 28.440347984214817 33.0 22.0 37.0 6.0 40.0 44-45 28.328904936501488 33.0 22.0 37.0 6.0 40.0 46-47 28.07881320596867 33.0 22.0 37.0 6.0 40.0 48-49 28.113779620757462 33.0 22.0 37.0 6.0 40.0 50-51 27.92358416209135 33.0 22.0 37.0 6.0 40.0 52-53 28.19010951172994 33.0 22.0 37.0 6.0 40.0 54-55 28.226078775276335 33.0 22.0 37.0 6.0 40.0 56-57 28.207133999209127 33.0 22.0 37.0 6.0 40.0 58-59 28.114416623401777 33.0 22.0 37.0 6.0 40.0 60-61 28.031280784201904 33.0 22.0 37.0 6.0 40.0 62-63 28.084260143486997 33.0 22.0 37.0 6.0 40.0 64-65 27.639852343432658 33.0 22.0 37.0 6.0 40.0 66-67 27.854660502877003 33.0 22.0 37.0 6.0 40.0 68-69 27.294154935828978 33.0 22.0 37.0 6.0 38.5 70-71 27.506635533879773 33.0 22.0 37.0 10.5 37.0 72-73 27.60875125423346 33.0 22.0 37.0 10.5 37.0 74-75 27.737429419945606 33.0 22.0 37.0 15.0 37.0 76-77 27.51615224040394 30.0 22.0 37.0 10.5 37.0 78-79 27.027299921181683 27.0 22.0 37.0 6.0 37.0 80-81 26.858330531203176 27.0 22.0 33.0 6.0 37.0 82-83 26.960000242104044 27.0 22.0 33.0 10.5 37.0 84-85 26.916754541468386 27.0 22.0 33.0 10.5 37.0 86-87 26.78587349794615 27.0 22.0 33.0 15.0 37.0 88-89 26.737599565288736 27.0 22.0 33.0 15.0 37.0 90-91 26.89909009229544 27.0 22.0 33.0 15.0 37.0 92-93 26.785349342687518 27.0 22.0 33.0 15.0 37.0 94-95 26.64345122006989 27.0 22.0 33.0 15.0 37.0 96-97 26.736743727487685 27.0 22.0 33.0 15.0 37.0 98-99 26.824930126082407 27.0 22.0 33.0 15.0 37.0 100 26.44480619571153 27.0 22.0 33.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.319312020921963 1101 2 11.221290850128398 1101 3 8.482774551671078 1101 4 12.865986234541278 1101 5 10.911323767014974 1101 6 10.042487576791086 1101 7 9.603714731201087 1101 8 9.149490338227878 1101 9 7.482138900137098 1101 10-11 7.916908090025839 1101 12-13 9.29196762298264 1101 14-15 8.243274329985184 1101 16-17 8.491476391936505 1101 18-19 8.359965073253257 1101 20-21 7.521678912068456 1101 22-23 7.604562002140728 1101 24-25 7.4013769393431765 1101 26-27 8.693347883383378 1101 28-29 8.338906406910237 1101 30-31 5.5691928472986625 1101 32-33 7.270207697959236 1101 34-35 8.812807429123957 1101 36-37 8.386415141108731 1101 38-39 8.285479344257062 1101 40-41 9.193360989054078 1101 42-43 8.786604482600001 1101 44-45 8.698010792463592 1101 46-47 7.477532355086062 1101 48-49 5.6157751194546925 1101 50-51 6.996633167360411 1101 52-53 6.103540168535119 1101 54-55 4.325554918267393 1101 56-57 6.384960415073934 1101 58-59 6.279166129899274 1101 60-61 6.907685763533966 1101 62-63 6.303526591253668 1101 64-65 6.765567814651693 1101 66-67 6.015728421264672 1101 68-69 7.0240529351615955 1101 70-71 6.513744406879102 1101 72-73 5.735988517547071 1101 74-75 5.072762810749527 1101 76-77 5.240964176838133 1101 78-79 5.194383579496272 1101 80-81 5.047397018321284 1101 82-83 4.630126043073528 1101 84-85 4.476678789640079 1101 86-87 4.264429843869326 1101 88-89 4.253731846100681 1101 90-91 4.005199027731237 1101 92-93 3.948637943545311 1101 94-95 3.856948350177383 1101 96-97 3.8703260995750206 1101 98-99 3.597339382140735 1101 100 3.9257368135976947 1102 1 6.648863315857557 1102 2 10.432981781180917 1102 3 8.132742293741686 1102 4 12.660752036720051 1102 5 10.65472491358965 1102 6 9.604233769116501 1102 7 6.791489142807155 1102 8 7.944770840217629 1102 9 6.689190752584285 1102 10-11 7.588306286492713 1102 12-13 9.139334971965873 1102 14-15 6.444037412657565 1102 16-17 7.5606094619478235 1102 18-19 7.557898584928772 1102 20-21 7.0838745038184605 1102 22-23 6.691457309735544 1102 24-25 7.019479690308767 1102 26-27 8.601094429704684 1102 28-29 7.8393375598555615 1102 30-31 4.401406992239178 1102 32-33 4.622528060838398 1102 34-35 5.096704772727669 1102 36-37 5.383763526533155 1102 38-39 4.695858868185827 1102 40-41 6.533513009205571 1102 42-43 7.132929031093713 1102 44-45 6.597935048275261 1102 46-47 5.719866206870265 1102 48-49 4.1559982760260255 1102 50-51 5.382008465134785 1102 52-53 6.107950225426507 1102 54-55 5.506358837554345 1102 56-57 6.487343824153825 1102 58-59 6.186692795495766 1102 60-61 6.1997152328049445 1102 62-63 4.466601180517966 1102 64-65 6.264350110653748 1102 66-67 4.036598754896215 1102 68-69 6.295024085915923 1102 70-71 5.535092627872224 1102 72-73 5.217444059787351 1102 74-75 4.452254080448181 1102 76-77 3.533580320116016 1102 78-79 4.547097856883507 1102 80-81 4.647247663433724 1102 82-83 4.081882937633512 1102 84-85 3.2312659332275864 1102 86-87 3.3741248195344156 1102 88-89 3.4250225161362486 1102 90-91 3.39465860819427 1102 92-93 3.400171123489887 1102 94-95 3.318564413866543 1102 96-97 3.3790318888704967 1102 98-99 3.159608687537027 1102 100 3.3812418864384206 1103 1 6.737791878141476 1103 2 10.888254107373807 1103 3 8.04651245197687 1103 4 11.891189287188634 1103 5 10.52000507435373 1103 6 9.264406908842655 1103 7 8.204046915267497 1103 8 8.173448499480301 1103 9 7.290030732542039 1103 10-11 7.587415144416365 1103 12-13 8.950033037905321 1103 14-15 8.715266415777727 1103 16-17 8.264813000065882 1103 18-19 7.325326750299197 1103 20-21 8.39888139746692 1103 22-23 7.0552939203056475 1103 24-25 5.437680815090534 1103 26-27 6.224083879899332 1103 28-29 7.217784415158267 1103 30-31 6.285487344945107 1103 32-33 8.222318518642496 1103 34-35 7.469752056199049 1103 36-37 4.424181973107551 1103 38-39 6.518377082826937 1103 40-41 7.000759584834576 1103 42-43 7.689184277949824 1103 44-45 5.9258739828694615 1103 46-47 6.632948252100682 1103 48-49 5.934066050802194 1103 50-51 6.733373099629713 1103 52-53 6.299888323471336 1103 54-55 5.199473497414594 1103 56-57 6.17408371919592 1103 58-59 5.769814475964232 1103 60-61 5.313083193183836 1103 62-63 2.9892796379047155 1103 64-65 5.290374019786221 1103 66-67 4.393058000334783 1103 68-69 5.569957028968453 1103 70-71 4.208731376899205 1103 72-73 4.832255712413097 1103 74-75 3.671025025478727 1103 76-77 3.8451897973014226 1103 78-79 3.238160864826856 1103 80-81 4.371932873374913 1103 82-83 3.743148002296511 1103 84-85 3.5169882626859206 1103 86-87 3.076038847074706 1103 88-89 3.315069542385384 1103 90-91 2.9654832773380306 1103 92-93 2.529296716307609 1103 94-95 3.1410193528301527 1103 96-97 3.3168847681881637 1103 98-99 3.0684708923063155 1103 100 3.2529137695826016 1104 1 6.288749865181604 1104 2 9.607987400000216 1104 3 7.384710541405106 1104 4 12.038139999583539 1104 5 9.462701622700198 1104 6 8.249129407499186 1104 7 4.17043835998901 1104 8 6.724601893983017 1104 9 6.713678980627773 1104 10-11 7.185159606331457 1104 12-13 7.633509334176871 1104 14-15 7.413922802879263 1104 16-17 7.248398089033497 1104 18-19 6.93965263542605 1104 20-21 7.7066252445131305 1104 22-23 6.640948700573549 1104 24-25 6.01602655479557 1104 26-27 8.288919084376996 1104 28-29 7.226144854849938 1104 30-31 6.338781558282566 1104 32-33 7.756424931120712 1104 34-35 8.02343837284301 1104 36-37 6.747221305523436 1104 38-39 7.284822941667265 1104 40-41 7.3799981550263 1104 42-43 7.3597358348831285 1104 44-45 6.874876544704655 1104 46-47 6.667925823603177 1104 48-49 5.591674262393855 1104 50-51 5.9858465074391916 1104 52-53 6.200912053500531 1104 54-55 4.956777757638477 1104 56-57 6.054848301540019 1104 58-59 3.8946699640066242 1104 60-61 4.271182002612871 1104 62-63 3.9784972634588094 1104 64-65 2.876483403182913 1104 66-67 3.3125614812745603 1104 68-69 4.392713779375761 1104 70-71 2.9318084633305794 1104 72-73 2.679495066651498 1104 74-75 3.1622765875300125 1104 76-77 3.8945638749980986 1104 78-79 4.306444550756101 1104 80-81 3.180439513247986 1104 82-83 3.063425116237049 1104 84-85 3.1149493237150345 1104 86-87 0.912116642915489 1104 88-89 0.5258879026544747 1104 90-91 2.2806228152551853 1104 92-93 2.5474248990537482 1104 94-95 2.6109415843563646 1104 96-97 2.9786661002003 1104 98-99 2.6096522775597 1104 100 1.0263566941122448 1105 1 5.887697515571929 1105 2 -2.873275645656104 1105 3 0.4737637572463598 1105 4 8.402087545608616 1105 5 8.914149429638396 1105 6 8.213320124558035 1105 7 5.504284532567386 1105 8 6.226954982498665 1105 9 5.245109097656009 1105 10-11 5.294419239244082 1105 12-13 5.396599998009442 1105 14-15 6.324688426357277 1105 16-17 6.315802690497389 1105 18-19 6.392146138689206 1105 20-21 7.350831494600104 1105 22-23 2.3094896656738086 1105 24-25 4.3217064596144645 1105 26-27 7.201867767983305 1105 28-29 6.372897356137106 1105 30-31 5.657789123023814 1105 32-33 7.419364091397902 1105 34-35 6.50959197413993 1105 36-37 6.589058986249267 1105 38-39 4.452907700166072 1105 40-41 5.823776203187386 1105 42-43 5.999587575347622 1105 44-45 6.524326887718445 1105 46-47 5.723799282476314 1105 48-49 5.370142015270858 1105 50-51 6.321117091745446 1105 52-53 5.9152495341929665 1105 54-55 5.560318218306694 1105 56-57 5.398104523448371 1105 58-59 2.200664703998001 1105 60-61 4.723746561217435 1105 62-63 -0.669654906294749 1105 64-65 -0.1458927674473145 1105 66-67 1.1535264945637316 1105 68-69 3.295535250438757 1105 70-71 3.311895182156679 1105 72-73 2.946390974490022 1105 74-75 2.9942544916670535 1105 76-77 2.104629729471199 1105 78-79 3.5051713381849545 1105 80-81 2.012001913231284 1105 82-83 1.3591128310555227 1105 84-85 1.7056262454271796 1105 86-87 0.0014057677084977627 1105 88-89 1.8639386467787133 1105 90-91 1.4774981631393516 1105 92-93 1.8733353119226805 1105 94-95 1.2079914396365474 1105 96-97 2.3156862809609144 1105 98-99 2.2681980086730675 1105 100 2.2731464190054425 1106 1 6.106288034684237 1106 2 10.323243763860045 1106 3 6.968396493116245 1106 4 11.799888217429665 1106 5 9.99319301237053 1106 6 9.474186422766987 1106 7 6.508983753701624 1106 8 7.475643873607744 1106 9 6.953290252580356 1106 10-11 5.869394031262491 1106 12-13 8.059179263573533 1106 14-15 7.9137835080932675 1106 16-17 7.902778790560273 1106 18-19 7.348684178184094 1106 20-21 8.403160116006863 1106 22-23 6.31447253432113 1106 24-25 5.16615151041243 1106 26-27 7.79629061224961 1106 28-29 6.463111915729957 1106 30-31 5.57862183018052 1106 32-33 7.497201175214336 1106 34-35 6.92197902097346 1106 36-37 6.3049560923477905 1106 38-39 7.220514499310724 1106 40-41 6.812074620352824 1106 42-43 6.433656214939393 1106 44-45 4.697048997112269 1106 46-47 6.1011927813777405 1106 48-49 5.98411504503936 1106 50-51 6.733236778654334 1106 52-53 5.897653733368344 1106 54-55 5.398000393724018 1106 56-57 4.989092029860558 1106 58-59 5.049403255460483 1106 60-61 4.975719752182695 1106 62-63 5.070301318712406 1106 64-65 5.294560145050557 1106 66-67 3.2939739918582305 1106 68-69 3.5559912525410304 1106 70-71 3.113290877363845 1106 72-73 3.0186716398632 1106 74-75 3.0816190171315228 1106 76-77 3.724307067306846 1106 78-79 4.330649324333788 1106 80-81 3.8317124559186233 1106 82-83 2.1955317108892203 1106 84-85 2.9526189185310834 1106 86-87 3.5815401571044383 1106 88-89 3.523117626386483 1106 90-91 3.2661172946750625 1106 92-93 3.0194484972292592 1106 94-95 1.769750784640415 1106 96-97 1.670306876700451 1106 98-99 2.1218136853929828 1106 100 3.0470201196714726 1107 1 6.005904629737628 1107 2 10.314246905755269 1107 3 6.278755898794337 1107 4 11.465047329335466 1107 5 9.48390254584481 1107 6 8.253210839228853 1107 7 4.013193277180303 1107 8 6.4864376453993735 1107 9 5.871374093040114 1107 10-11 4.700811918402682 1107 12-13 6.355829509055603 1107 14-15 6.3748997772245914 1107 16-17 7.124571964910064 1107 18-19 6.435026880397508 1107 20-21 5.72268491213762 1107 22-23 5.926310092406201 1107 24-25 4.621216018073479 1107 26-27 7.338585873713168 1107 28-29 6.553838785832728 1107 30-31 5.159608956177177 1107 32-33 5.264993062262107 1107 34-35 6.787235467069486 1107 36-37 6.633457820930911 1107 38-39 6.485727210566061 1107 40-41 7.852910606343251 1107 42-43 6.370337620471322 1107 44-45 4.342811731750757 1107 46-47 3.6623229788022833 1107 48-49 4.336423503970394 1107 50-51 5.027984976415123 1107 52-53 4.05475331214398 1107 54-55 5.424061885336073 1107 56-57 5.412963551351261 1107 58-59 3.2082569470972473 1107 60-61 3.084310723436989 1107 62-63 0.792632338201205 1107 64-65 2.4267906088168374 1107 66-67 4.373446080050218 1107 68-69 5.231860091278595 1107 70-71 4.460559620995749 1107 72-73 2.6071117463998945 1107 74-75 2.844928533629666 1107 76-77 3.3291366456269493 1107 78-79 4.049067083333679 1107 80-81 2.3626626309726895 1107 82-83 1.8809270105982314 1107 84-85 2.5826589697478717 1107 86-87 2.464156696772381 1107 88-89 2.6580160137523094 1107 90-91 1.601558918248685 1107 92-93 2.491837687825008 1107 94-95 2.4361436156004217 1107 96-97 2.4122678918993294 1107 98-99 2.024152950431361 1107 100 0.38772829308888035 1108 1 4.480618595114056 1108 2 -3.303365419115414 1108 3 0.06658155287608025 1108 4 6.703363523744535 1108 5 7.19137598220405 1108 6 7.086379937082583 1108 7 4.546792128357893 1108 8 5.326022110079688 1108 9 5.140463410209836 1108 10-11 3.45862214913112 1108 12-13 2.6292063441956586 1108 14-15 4.17842472803148 1108 16-17 4.857222938485261 1108 18-19 4.149824675088183 1108 20-21 4.85603529194249 1108 22-23 4.1605359169633225 1108 24-25 2.970452739530245 1108 26-27 4.879019621954104 1108 28-29 6.1912159988832975 1108 30-31 4.805184030004813 1108 32-33 4.7206568603363905 1108 34-35 3.167580730854713 1108 36-37 1.3418109783141752 1108 38-39 2.71611532499238 1108 40-41 5.619427262461784 1108 42-43 3.3067965221424878 1108 44-45 3.833263280472515 1108 46-47 4.929792779930867 1108 48-49 5.104347501112727 1108 50-51 2.3970117241353925 1108 52-53 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-18.229845021118237 2213 54-55 -18.131860472426016 2213 56-57 -18.03743686059608 2213 58-59 -18.463246978914235 2213 60-61 -19.81970330417997 2213 62-63 -18.02648943866885 2213 64-65 -18.12944262598953 2213 66-67 -8.966618872531722 2213 68-69 -23.142725050411475 2213 70-71 -17.81948158097055 2213 72-73 -9.587570473400337 2213 74-75 -4.487852812445876 2213 76-77 -3.4961200801583097 2213 78-79 -4.94192937938675 2213 80-81 -3.464960477710971 2213 82-83 -3.3151924324339177 2213 84-85 -3.29219065428509 2213 86-87 -3.465192779361651 2213 88-89 -2.998169379908358 2213 90-91 -3.174956828828961 2213 92-93 -3.98877243685126 2213 94-95 -4.068990683138061 2213 96-97 -3.760057831449391 2213 98-99 -4.054750297631671 2213 100 -3.4608562526661295 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1050.0 3 1235.0 4 9693.0 5 31338.0 6 33041.0 7 38164.0 8 34520.0 9 33952.0 10 39164.0 11 46576.0 12 57115.0 13 75756.0 14 57377.0 15 63510.0 16 72317.0 17 76287.0 18 76371.0 19 76037.0 20 73154.0 21 70698.0 22 72602.0 23 79520.0 24 87613.0 25 97292.0 26 106670.0 27 115129.0 28 125491.0 29 130506.0 30 135922.0 31 148573.0 32 169182.0 33 204875.0 34 250951.0 35 299353.0 36 333936.0 37 296876.0 38 95372.0 39 192.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.25468787016853 21.09490171303753 13.745781475593274 23.904628941200663 2 15.213785029839563 21.25138966478635 40.43538072644762 23.099444578926473 3 17.894289731018244 26.87439026910985 29.35200472092921 25.879315278942695 4 12.399517120345852 17.842188837584775 39.33314260621948 30.425151435849894 5 13.231082969591682 37.5656669827449 36.04685550400845 13.156394543654976 6 29.999697104409943 38.78868688030698 18.59772803831048 12.613887976972594 7 26.638592789210403 31.725017946386245 24.15732275007033 17.479066514333024 8 23.749744993728957 36.15349144325703 22.053485528318674 18.04327803469534 9 24.40717220877125 17.34606958753606 22.21518066632003 36.031577537372655 10-11 23.09621130849384 27.477353797086856 29.309049426493498 20.117385467925804 12-13 24.04896962635497 25.059453009389763 29.321776451794275 21.569800912460995 14-15 21.446466265224174 26.57551076428201 26.592463322141686 25.385559648352128 16-17 21.060564129602373 30.203827783728848 27.389591419962358 21.34601666670643 18-19 21.058714366282977 29.296692944364366 28.913115820970646 20.73147686838201 20-21 22.345262985282663 28.640317825903853 27.95553120679181 21.058887982021666 22-23 21.362651544581 28.659795753460234 28.43426659982392 21.543286102134847 24-25 21.687942043317143 28.494819054675663 27.92354265690659 21.893696245100603 26-27 21.618115959794626 28.437883880619154 28.07353087407109 21.870469285515128 28-29 21.441501045448902 28.339202020943414 28.523996003523695 21.69530093008399 30-31 22.003099318022258 28.785849618477215 27.804247471244704 21.406803592255823 32-33 21.124754767915555 28.627621097447093 28.455844105319905 21.791780029317447 34-35 21.243506807674063 28.074826002506413 28.341456953173495 22.340210236646033 36-37 21.893182427594418 28.68615063661517 27.844642915022703 21.576024020767708 38-39 21.631444602124787 28.341194076572133 28.146581042168144 21.880780279134935 40-41 21.81815803130274 28.37362504832735 27.790102829174483 22.01811409119542 42-43 21.53239252339721 28.46760747660279 28.28968830370008 21.71031169629992 44-45 21.943002195261233 28.01539783844001 28.127662163806743 21.91393780249202 46-47 21.756719809300424 28.47502629204931 28.26772457286945 21.500529325780814 48-49 21.600170099114685 28.211896674401142 28.36702446574359 21.820908760740583 50-51 21.8844833597669 28.279415511005247 28.04023673987845 21.795864389349408 52-53 21.31426916321496 28.324118477130273 28.24654246705124 22.115069892603525 54-55 21.762139923229594 28.32487807823051 27.908665218965616 22.004316779574282 56-57 21.90826392209165 28.252386595261353 27.849931027225374 21.989418455421625 58-59 21.55688208883855 27.79568064839643 28.375539002100293 22.27189826066473 60-61 22.019684729562236 28.282864438938905 27.847321473974095 21.850129357524768 62-63 21.415821922166298 28.432071044491554 28.279880290188014 21.872226743154137 64-65 21.49120965059936 27.852493635239945 28.314981561207826 22.341315152952873 66-67 21.665771418974533 28.14435279143782 27.996963097751333 22.192912691836312 68-69 22.119214073171506 28.016891492898026 27.9623916556486 21.901502778281873 70-71 22.122975160408558 28.13596431393359 27.727335243999747 22.013725281658104 72-73 21.764161425511695 28.26849830517093 28.162953709487127 21.80438655983025 74-75 22.093785351313496 27.788342929029934 27.827588158252 22.29028356140457 76-77 22.021206533822347 28.211591235601286 27.924439621488045 21.84276260908832 78-79 22.027362742320456 27.959834461218403 28.150733792967493 21.86206900349365 80-81 22.088579256195803 27.917571639991845 27.887887180275932 22.10596192353642 82-83 21.539223471842124 28.198245504768654 28.243164769341078 22.01936625404815 84-85 21.799957485439037 27.788059434917933 27.885374497947414 22.52660858169562 86-87 22.04311986307343 28.06656660466536 27.8705709145154 22.019742617745802 88-89 21.806693504802872 27.651959785944403 28.26064155077009 22.280705158482636 90-91 22.13638983121335 27.725763147789184 27.923264213136424 22.214582807861046 92-93 21.65563996106248 28.041236109299934 28.19409671287476 22.10902721676283 94-95 21.643890089242372 27.56353980875026 28.142819807328912 22.649750294678455 96-97 21.89647185561312 27.83227115988906 28.053502297473294 22.217754687024524 98-99 21.83691954665355 27.593584160654856 28.139431746667643 22.430064546023953 100 21.95840147664579 27.45600260584551 27.954777722735326 22.63081819477338 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 395.0 1 477.0 2 463.0 3 380.0 4 477.5 5 786.5 6 1278.5 7 2057.0 8 3079.0 9 4366.0 10 6021.0 11 7653.5 12 9103.5 13 10398.0 14 11509.5 15 12493.5 16 13602.0 17 14640.5 18 15682.0 19 16723.5 20 17619.5 21 19017.5 22 20735.5 23 22757.5 24 25410.0 25 28781.5 26 33328.5 27 39552.0 28 47653.0 29 57095.0 30 67582.5 31 78889.5 32 90383.5 33 101188.5 34 110604.5 35 119147.5 36 127133.5 37 133943.0 38 138735.5 39 141841.0 40 142966.0 41 142235.0 42 140225.5 43 136370.5 44 130088.0 45 123611.0 46 118530.0 47 113459.0 48 108721.5 49 105820.0 50 104289.5 51 97997.5 52 87136.0 53 78012.5 54 71111.5 55 65078.5 56 59295.0 57 53510.0 58 47792.5 59 42718.5 60 37109.0 61 31189.5 62 26238.0 63 21398.0 64 17269.0 65 13980.0 66 11046.0 67 8775.5 68 6933.0 69 5354.5 70 4154.0 71 3195.5 72 2452.5 73 1830.5 74 1355.5 75 1003.5 76 697.5 77 499.5 78 359.5 79 238.0 80 164.0 81 113.0 82 76.5 83 57.0 84 40.0 85 31.0 86 24.0 87 14.0 88 12.5 89 9.5 90 9.0 91 5.0 92 3.0 93 3.5 94 1.5 95 1.5 96 1.5 97 1.0 98 0.0 99 2.0 100 4.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 9.729220075267458 2 27.433535714381783 3 7.599188682442884 4 27.222555488902216 5 14.495899026472733 6 12.077145109094772 7 9.539329802200996 8 10.861486895446022 9 6.616300058373975 10-11 8.199445312731175 12-13 11.98975899887287 14-15 9.155258634371782 16-17 9.795475882401995 18-19 7.967765191356348 20-21 9.77488358830476 22-23 7.0354763128091875 24-25 6.258914136455219 26-27 11.672602699191103 28-29 7.825112107623318 30-31 5.263543703815291 32-33 10.455545662168014 34-35 8.815438705980776 36-37 8.707137496267563 38-39 7.534170295985645 40-41 10.556529949615458 42-43 7.808985288144164 44-45 8.046946664478764 46-47 7.367454760169043 48-49 4.859525852676998 50-51 8.34252880365631 52-53 5.73262029208508 54-55 5.983830139801636 56-57 5.928267261345937 58-59 4.644766114041766 60-61 5.308023597074307 62-63 3.606758468934016 64-65 5.200341097699742 66-67 3.0950312179716524 68-69 6.6132065066807275 70-71 3.3798800777961 72-73 2.1288343228215343 74-75 1.1245463911701965 76-77 1.4998480124602882 78-79 2.2970562838105026 80-81 2.5820799965567423 82-83 1.3075097984887327 84-85 1.799532470187577 86-87 0.5210482567163698 88-89 0.8737131497467323 90-91 0.38715126929770993 92-93 0.571150343922247 94-95 0.8275788788430655 96-97 0.8155140272393951 98-99 0.55389370556382 100 0.8980715067748782 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3717410.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 95.22352755920113 #Duplication Level Percentage of deduplicated Percentage of total 1 97.08873690327025 92.45132014196581 2 2.3254585875195533 4.428767397928982 3 0.3199673333331506 0.9140525455108008 4 0.10859827070479093 0.4136444169335697 5 0.05195667034054531 0.2473748715028622 6 0.026033040309571865 0.14873747588209862 7 0.01922286964871743 0.12813286204331029 8 0.013178341239307036 0.10039105121485681 9 0.008773953967801113 0.0751938162710464 >10 0.03613410555586455 0.557553087220451 >50 8.535468430732519E-4 0.05424940907508841 >100 8.022613523097769E-4 0.1764630798198613 >500 1.9888114641379145E-4 0.1359760165270363 >1k 8.523476862223634E-5 0.16814382810422096 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 2.6900449506511254E-5 0.0 4 0.0 0.0 0.0 5.380089901302251E-5 0.0 5 2.6900449506511254E-5 0.0 0.0 5.380089901302251E-5 0.0 6 2.6900449506511254E-5 0.0 0.0 5.380089901302251E-5 0.0 7 2.6900449506511254E-5 0.0 0.0 5.380089901302251E-5 0.0 8 2.6900449506511254E-5 0.0 0.0 5.380089901302251E-5 0.0 9 2.6900449506511254E-5 0.0 0.0 5.380089901302251E-5 0.0 10-11 2.6900449506511254E-5 0.0 0.0 8.070134851953376E-5 0.0 12-13 2.6900449506511254E-5 0.0 0.0 1.2105202277930064E-4 0.0 14-15 2.6900449506511254E-5 0.0 0.0 1.3450224753255627E-4 0.0 16-17 2.6900449506511254E-5 5.380089901302251E-5 0.0 1.6140269703906752E-4 0.0 18-19 2.6900449506511254E-5 6.725112376627813E-5 0.0 1.6140269703906752E-4 0.0 20-21 5.380089901302251E-5 8.070134851953376E-5 0.0 1.6140269703906752E-4 0.0 22-23 5.380089901302251E-5 8.070134851953376E-5 0.0 1.6140269703906752E-4 0.0 24-25 8.070134851953376E-5 8.070134851953376E-5 0.0 1.7485292179232316E-4 0.0 26-27 8.070134851953376E-5 8.070134851953376E-5 0.0 2.2865382080534567E-4 0.0 28-29 8.070134851953376E-5 8.070134851953376E-5 0.0 4.70757866363947E-4 0.0 30-31 9.415157327278939E-5 8.070134851953376E-5 0.0 0.0014929749476113746 0.0 32-33 1.0760179802604501E-4 9.415157327278939E-5 0.0 0.004640327539873191 0.0 34-35 1.0760179802604501E-4 1.3450224753255627E-4 0.0 0.010060768115435208 0.0 36-37 1.3450224753255627E-4 1.3450224753255627E-4 0.0 0.018198154091154863 0.0 38-39 1.3450224753255627E-4 1.3450224753255627E-4 0.0 0.03214603716028095 0.0 40-41 1.3450224753255627E-4 1.3450224753255627E-4 0.0 0.04936232484444815 0.0 42-43 1.3450224753255627E-4 1.7485292179232316E-4 0.0 0.06752012826134324 0.0 44-45 1.3450224753255627E-4 2.017533712988344E-4 0.0 0.08904048786655225 0.0 46-47 1.3450224753255627E-4 2.6900449506511254E-4 0.0 0.11637134456516768 0.0 48-49 1.3450224753255627E-4 2.959049445716238E-4 0.0 0.14432091160243288 0.0 50-51 1.3450224753255627E-4 3.2280539407813504E-4 0.0 0.17739501427068846 2.6900449506511254E-5 52-53 1.3450224753255627E-4 3.2280539407813504E-4 0.0 0.2101463115448659 5.380089901302251E-5 54-55 1.3450224753255627E-4 3.2280539407813504E-4 0.0 0.24896366018276167 5.380089901302251E-5 56-57 1.3450224753255627E-4 3.2280539407813504E-4 0.0 0.2931207480476999 6.725112376627813E-5 58-59 1.3450224753255627E-4 3.2280539407813504E-4 0.0 0.34493101379724056 8.070134851953376E-5 60-61 1.3450224753255627E-4 3.2280539407813504E-4 0.0 0.40046699180343304 8.070134851953376E-5 62-63 1.3450224753255627E-4 3.362556188313907E-4 0.0 0.45838365959095173 8.070134851953376E-5 64-65 1.3450224753255627E-4 3.497058435846463E-4 0.0 0.5241821590838782 9.415157327278939E-5 66-67 1.3450224753255627E-4 3.7660629309115755E-4 0.0 0.6024893675973326 1.0760179802604501E-4 68-69 1.3450224753255627E-4 3.7660629309115755E-4 0.0 0.6906555908549232 1.0760179802604501E-4 70-71 1.3450224753255627E-4 3.7660629309115755E-4 0.0 0.7962667556174864 1.0760179802604501E-4 72-73 1.3450224753255627E-4 4.035067425976688E-4 0.0 0.9085492318576643 1.0760179802604501E-4 74-75 1.4795247228581188E-4 4.3040719210418006E-4 0.0 1.0249878275465982 1.0760179802604501E-4 76-77 1.6140269703906752E-4 4.3040719210418006E-4 0.0 1.1547555959660087 1.0760179802604501E-4 78-79 1.8830314654557878E-4 4.3040719210418006E-4 0.0 1.3033940297142363 1.0760179802604501E-4 80-81 1.8830314654557878E-4 4.3040719210418006E-4 0.0 1.4746557414974404 1.2105202277930064E-4 82-83 1.8830314654557878E-4 4.3040719210418006E-4 0.0 1.659044872639822 1.3450224753255627E-4 84-85 1.8830314654557878E-4 4.3040719210418006E-4 0.0 1.8521362991975596 1.3450224753255627E-4 86-87 1.8830314654557878E-4 4.8420809111720257E-4 0.0 2.061085540739386 1.3450224753255627E-4 88 1.8830314654557878E-4 5.111085406237138E-4 0.0 2.235373553092072 1.3450224753255627E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATAGG 970 0.0 18.924341 1 GTATTAG 1455 0.0 16.531609 1 GTGTTAG 1660 0.0 15.252688 1 ACACCGT 470 1.6534614E-9 15.122886 6 GTACATG 12780 0.0 15.007427 1 TATACTG 1825 0.0 14.976502 5 GTATAAG 1320 0.0 14.865593 1 TACATGG 12990 0.0 14.762368 2 GTATATA 2155 0.0 14.686464 1 GTACCGT 245 7.788129E-4 14.505627 6 GTATTAT 1945 0.0 14.3194895 1 ACATGGG 13280 0.0 14.211602 3 TAGACCG 220 0.0065192417 13.804099 5 GTATAGA 1540 0.0 13.563998 1 TCTGTCG 725 1.8189894E-12 13.314005 8 CATGGGG 4785 0.0 13.266863 4 ACAGCGC 540 1.1557859E-8 13.171263 8 GTATAAA 2385 0.0 13.004755 1 GTCTTGC 1960 0.0 12.59514 1 CTGTCGC 825 1.8189894E-12 12.31967 9 >>END_MODULE