FastQCFastQC Report
Wed 25 May 2016
SRR1295136_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295136_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3717410
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT81160.21832404819484533No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA59220.15930446197755962No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA132850.016.0017971
TAGACCG2751.2457705E-515.3850015
GTACTAG7900.015.3038951
GTACATG173150.014.9831311
TACATGG176500.014.5149222
CAACGCA143350.014.42921455
GAGTACT39350.014.3739112-13
TCAACGC144300.014.3667974
AACGCAG144050.014.3388056
ACATGGG168250.014.3332323
AGACCGT4603.6889105E-914.2870886
ATCAACG145650.014.2334423
TATCAAC146350.014.2036182
CTGTCGC6950.014.1831589
CATGGGG54600.013.947944
TATACTG20900.013.4956145
TAGACAG21000.013.4313515
ACACCGT4554.9161827E-813.412376
TATCCCG3901.6922768E-613.2591545
CCGTGCG2852.934848E-413.1769
Produced by FastQC (version 0.11.5)