FastQCFastQC Report
Wed 25 May 2016
SRR1295135_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295135_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2963270
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG6601.8189894E-1216.0867561
GTGTATA17650.015.9232351
AGTATCG1900.002626926415.8268485
GTATTAT17650.015.5693861
TATACCG2607.707022E-515.4210315
GTATATA24650.014.9485331
CTCGTAC2300.00429230914.7015133
GTAGCGA2050.00433736314.678538
GTACATG121550.014.5410231
TATACAG15650.014.4110295
TACATGG123300.014.1288442
GTATAGG8055.456968E-1213.9649731
GTCTCGC4607.2339662E-613.5770581
ACATGGG126700.013.0770363
CATGGGT18550.013.0639044
GTATAGC5753.8222788E-713.0339761
GTCTAGG7202.57387E-913.0113471
TATTCCG5501.8060746E-812.75745
CTATACT12900.012.6694194
GTGTAAG12450.012.5410571
Produced by FastQC (version 0.11.5)