FastQCFastQC Report
Wed 25 May 2016
SRR1295135_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295135_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2963270
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT87310.2946407178556122No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA61200.20652859847398314No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG2551.346416E-820.276925
ATACCGC1650.001581355217.0759146
GAGTACT37400.016.25731312-13
ATACGGC1800.00282797815.6529196
TAACGGT1850.003371828715.2450554
CATGGGT22150.014.6428234
GTATCAA138900.014.2856981
AGTACTT44400.014.06433912-13
TATACTG19200.013.9547835
TCCGTAC2701.834447E-413.9273453
TAGACAG17050.013.5089375
CATGGGG34200.013.3319944
GTGTATA20200.013.2945361
TATACAG20800.013.1073275
ACACCGT4303.5807716E-713.104776
ACTGTCG2150.00920185913.1030018
CTAATAC14750.013.065743
TATCAAC151350.013.0024532
GTACTTT48200.012.95542114-15
GTACATG160450.012.9248981
Produced by FastQC (version 0.11.5)