FastQCFastQC Report
Wed 25 May 2016
SRR1295134_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295134_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1442992
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT16060.11129652832448135No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG5000.023.330865
TATGTCG1859.202308E-519.1910725
CATGGGG23350.018.6574384
GTACATG83250.018.2662731
GAGTACT10550.017.99040212-13
TACATGG84000.017.796372
GTATCAA65950.017.269291
GTACAAG6850.016.7193031
GTCTAAC2702.9289396E-416.4956931
ACGCGGA2203.6099725E-416.0498397
ACTGTCG1900.002402265816.0393098
CACACCG2254.1085496E-415.7793255
GTATAGA4452.452307E-715.7278371
ACATGGG86050.015.7212273
GGTATCA55150.015.1133971
GTCCAGA5501.0879376E-815.038931
GTCATAT5551.225817E-814.9034451
CCCTAAG3451.122424E-414.7539121
TATCAAC73200.014.5996032
GTGTTAC3551.4313043E-414.3383071
Produced by FastQC (version 0.11.5)