##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295134_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1442992 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.3277287746571 27.0 22.0 33.0 2.0 33.0 2 21.242617422688415 27.0 2.0 33.0 2.0 33.0 3 23.636725636732567 27.0 15.0 33.0 6.0 33.0 4 23.263181639260647 33.0 2.0 37.0 2.0 37.0 5 25.31984307605309 33.0 15.0 37.0 2.0 37.0 6 26.269122074134852 33.0 15.0 37.0 2.0 37.0 7 26.149815106390058 33.0 15.0 37.0 2.0 37.0 8 26.806630251588366 33.0 15.0 37.0 2.0 37.0 9 27.79792680763303 33.0 15.0 37.0 6.0 37.0 10-11 27.335672685642052 33.0 15.0 37.0 4.0 37.0 12-13 26.08387052734873 33.0 15.0 37.0 2.0 37.0 14-15 27.758429014159468 33.0 15.0 37.0 4.0 40.0 16-17 28.193225949970618 33.0 15.0 37.0 4.0 40.0 18-19 28.461530278754143 33.0 15.0 37.0 6.0 40.0 20-21 27.914246925831883 33.0 15.0 37.0 4.0 40.0 22-23 28.761072479958308 33.0 18.5 37.0 6.0 40.0 24-25 28.96731755962611 33.0 22.0 37.0 6.0 40.0 26-27 27.418003703416232 33.0 15.0 37.0 2.0 40.0 28-29 28.204896839344915 33.0 15.0 37.0 6.0 40.0 30-31 29.00578658786743 33.0 22.0 37.0 6.0 40.0 32-33 27.665141248184327 33.0 15.0 37.0 2.0 40.0 34-35 27.325391270360473 33.0 15.0 37.0 6.0 40.0 36-37 27.611101101045605 33.0 15.0 37.0 6.0 40.0 38-39 27.730955888875336 33.0 18.5 37.0 6.0 40.0 40-41 26.780821376695087 33.0 15.0 37.0 4.0 40.0 42-43 27.375098060141703 33.0 15.0 37.0 6.0 40.0 44-45 27.292627748455985 33.0 15.0 37.0 6.0 40.0 46-47 27.021151537915664 33.0 15.0 37.0 6.0 40.0 48-49 27.053382485834987 33.0 15.0 37.0 6.0 40.0 50-51 26.884430405712575 33.0 15.0 37.0 6.0 40.0 52-53 27.16084704558307 33.0 18.5 37.0 6.0 40.0 54-55 27.113676998902278 33.0 18.5 37.0 6.0 40.0 56-57 27.1060778576735 33.0 18.5 37.0 6.0 40.0 58-59 26.999602561899167 33.0 18.5 37.0 6.0 40.0 60-61 26.944396781132536 33.0 18.5 37.0 6.0 40.0 62-63 27.018005643828936 33.0 22.0 37.0 6.0 40.0 64-65 26.543359907747238 30.0 18.5 37.0 6.0 38.5 66-67 26.732323186822935 27.0 18.5 37.0 6.0 37.0 68-69 26.23047702274164 30.0 18.5 37.0 6.0 37.0 70-71 26.430623662501247 27.0 18.5 37.0 6.0 37.0 72-73 26.538905621098387 27.0 22.0 37.0 6.0 37.0 74-75 26.682422355771898 27.0 22.0 35.0 6.0 37.0 76-77 26.44080909665473 27.0 22.0 33.0 6.0 37.0 78-79 25.99359213356692 27.0 22.0 33.0 6.0 37.0 80-81 25.834251679842993 27.0 22.0 33.0 6.0 37.0 82-83 25.98123828822336 27.0 22.0 33.0 6.0 37.0 84-85 25.92576639371528 27.0 22.0 33.0 6.0 37.0 86-87 25.804542575426616 27.0 22.0 33.0 6.0 37.0 88-89 25.780324838945745 27.0 22.0 33.0 6.0 37.0 90-91 25.93682189506248 27.0 22.0 33.0 6.0 37.0 92-93 25.809449394036832 27.0 22.0 33.0 6.0 37.0 94-95 25.65277111723419 27.0 22.0 33.0 6.0 37.0 96-97 25.721366438621974 27.0 22.0 33.0 6.0 37.0 98-99 25.81925298269152 27.0 22.0 33.0 6.0 37.0 100 25.442958796722365 27.0 22.0 33.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.52862936398693 1101 2 11.278659242794955 1101 3 8.552862903029649 1101 4 12.667607173839599 1101 5 10.935704683910455 1101 6 10.113660402522811 1101 7 9.62593963242535 1101 8 9.05014229466321 1101 9 7.367159687335345 1101 10-11 7.851706770441361 1101 12-13 9.213557751436642 1101 14-15 8.438525206821836 1101 16-17 8.417417473247692 1101 18-19 8.296113384239941 1101 20-21 7.280015580138919 1101 22-23 7.527450350835412 1101 24-25 7.525803351144365 1101 26-27 8.76576268404538 1101 28-29 8.369514520195924 1101 30-31 5.833808184385102 1101 32-33 7.356699935641988 1101 34-35 9.10186358189949 1101 36-37 8.631158588167484 1101 38-39 8.514732156271688 1101 40-41 9.367034239910723 1101 42-43 8.869755990939428 1101 44-45 8.64356127826402 1101 46-47 7.467620821714672 1101 48-49 5.57651372420597 1101 50-51 6.974936826341995 1101 52-53 6.292500829589358 1101 54-55 4.362525101302285 1101 56-57 6.426202151247757 1101 58-59 6.329936539160084 1101 60-61 7.02185593456986 1101 62-63 6.324622905959149 1101 64-65 6.812321777382305 1101 66-67 6.076738010671509 1101 68-69 7.137482478744914 1101 70-71 6.7102402447791505 1101 72-73 6.0462439101491405 1101 74-75 5.305523969187792 1101 76-77 5.373197248659402 1101 78-79 5.337844216684715 1101 80-81 5.214821160909221 1101 82-83 4.819342283644769 1101 84-85 4.708915725775885 1101 86-87 4.534011316248133 1101 88-89 4.38131229587114 1101 90-91 4.173805251756505 1101 92-93 4.1657093492990676 1101 94-95 4.087183593018459 1101 96-97 4.251997386753377 1101 98-99 3.981453858953529 1101 100 4.120464187470663 1102 1 6.5888398934776475 1102 2 10.46859952239501 1102 3 7.994948441945937 1102 4 12.301129772581852 1102 5 10.220466179333403 1102 6 9.243612596041032 1102 7 6.35290354380993 1102 8 7.620091321190529 1102 9 6.255029358119099 1102 10-11 7.162695851863191 1102 12-13 8.7502024753806 1102 14-15 5.886215188573935 1102 16-17 6.9737578644464655 1102 18-19 7.010648741431265 1102 20-21 6.764150463789708 1102 22-23 6.295620961159802 1102 24-25 6.655797017162552 1102 26-27 8.138085822684179 1102 28-29 7.291578267198592 1102 30-31 4.02151837455537 1102 32-33 4.179712271825611 1102 34-35 4.959752405437776 1102 36-37 4.978206123192901 1102 38-39 4.360652393343578 1102 40-41 6.0308788914663225 1102 42-43 6.771466166028777 1102 44-45 6.159812842908341 1102 46-47 5.220989519779792 1102 48-49 3.5902543174889345 1102 50-51 4.7055276963596455 1102 52-53 5.739557413160213 1102 54-55 5.15130377388067 1102 56-57 5.9791116356314795 1102 58-59 5.761292011267944 1102 60-61 5.876647516406411 1102 62-63 4.215795295830812 1102 64-65 5.757223676068758 1102 66-67 3.5436863133151917 1102 68-69 5.7786002003211685 1102 70-71 4.990458616342586 1102 72-73 4.852156380552856 1102 74-75 3.9951286985608796 1102 76-77 3.3957092756843927 1102 78-79 4.253760608124793 1102 80-81 4.458871742278266 1102 82-83 3.6944516202355793 1102 84-85 2.9875751083634654 1102 86-87 3.0671666948543574 1102 88-89 3.1549082632360523 1102 90-91 3.126272111458807 1102 92-93 3.2529147060379735 1102 94-95 3.0650881761067232 1102 96-97 3.256602719362828 1102 98-99 3.0883096402200216 1102 100 3.2952820854935574 1103 1 6.524717197385989 1103 2 10.641265863649974 1103 3 7.6410732874612926 1103 4 11.524164299521999 1103 5 10.102665839203514 1103 6 8.806441881340476 1103 7 7.76603432080088 1103 8 7.656545727230622 1103 9 6.778905938205632 1103 10-11 7.071138910918073 1103 12-13 8.365684286750604 1103 14-15 8.17578526946825 1103 16-17 7.594761898200641 1103 18-19 6.841909560096479 1103 20-21 7.876944784514105 1103 22-23 6.408515299173924 1103 24-25 4.797868555358551 1103 26-27 5.491167652825194 1103 28-29 6.578781232734936 1103 30-31 5.90531303763753 1103 32-33 7.517806677007737 1103 34-35 6.814197148907059 1103 36-37 4.092953151144769 1103 38-39 6.26043240270776 1103 40-41 6.4976018119332615 1103 42-43 7.287675721685378 1103 44-45 5.6884657225945325 1103 46-47 6.267180502320496 1103 48-49 5.293713541067987 1103 50-51 6.321807572229183 1103 52-53 6.016591440724934 1103 54-55 4.878380468773777 1103 56-57 5.798656092427979 1103 58-59 5.35823631716158 1103 60-61 4.927228712847832 1103 62-63 2.7292400963004617 1103 64-65 4.883873768780283 1103 66-67 4.0101818840542585 1103 68-69 4.867054531466803 1103 70-71 3.7013488045872123 1103 72-73 4.312876308322359 1103 74-75 3.291227514695091 1103 76-77 3.4944285329588425 1103 78-79 2.869871082672468 1103 80-81 4.087302474557699 1103 82-83 3.457852663140983 1103 84-85 3.280613324329945 1103 86-87 2.705766187349564 1103 88-89 2.9525818776677397 1103 90-91 2.6228188486258155 1103 92-93 2.2236402734079697 1103 94-95 2.7226070528888613 1103 96-97 2.9226751482341236 1103 98-99 2.6591034539847485 1103 100 2.930233079984191 1104 1 6.489376147643881 1104 2 9.708625951035877 1104 3 7.4433555822397715 1104 4 11.905311546901423 1104 5 9.201445959382777 1104 6 8.055202791279317 1104 7 4.119273758419617 1104 8 6.512171490468251 1104 9 6.5851937749906995 1104 10-11 6.912562868511316 1104 12-13 7.497262139057543 1104 14-15 7.397429417792587 1104 16-17 7.3200440523010535 1104 18-19 6.852935647011886 1104 20-21 7.433854323787518 1104 22-23 6.463516463610031 1104 24-25 6.005296889340105 1104 26-27 8.100375800804333 1104 28-29 7.220522129740861 1104 30-31 6.305511523752369 1104 32-33 7.497388555781075 1104 34-35 7.96284864913817 1104 36-37 6.6243491859147134 1104 38-39 7.413397695418627 1104 40-41 7.150540624066565 1104 42-43 7.341984549056836 1104 44-45 6.628927099371776 1104 46-47 6.648355885313581 1104 48-49 5.497250826075845 1104 50-51 5.782249608727454 1104 52-53 5.908298764141449 1104 54-55 4.989669410886528 1104 56-57 5.94309517114031 1104 58-59 3.7753514000966213 1104 60-61 4.314637907519089 1104 62-63 3.8048718176680474 1104 64-65 2.7974795249958326 1104 66-67 3.3889051537435684 1104 68-69 4.285322760802789 1104 70-71 2.767931320549444 1104 72-73 2.6368503928214118 1104 74-75 3.4308220663162565 1104 76-77 3.9120049572060687 1104 78-79 4.309579950001229 1104 80-81 3.221732840687146 1104 82-83 3.0270744888113015 1104 84-85 3.0434815255303214 1104 86-87 0.9232713268277308 1104 88-89 0.762426623460108 1104 90-91 2.4572861575252993 1104 92-93 2.6821196144198822 1104 94-95 2.5473617233416164 1104 96-97 2.9444578467231928 1104 98-99 2.6166361143488786 1104 100 0.8634600575454492 1105 1 5.787567308574616 1105 2 -2.755229268168158 1105 3 0.46863374563616134 1105 4 8.175404809050715 1105 5 8.545334652584607 1105 6 7.7862875652147245 1105 7 5.084945183735076 1105 8 5.84496242532288 1105 9 4.726968587534014 1105 10-11 4.798958013630532 1105 12-13 4.991234632363895 1105 14-15 5.655905430012666 1105 16-17 5.935565259022564 1105 18-19 5.710996917780616 1105 20-21 6.829359622302366 1105 22-23 1.9572887805678114 1105 24-25 4.016395798787649 1105 26-27 6.92136300747519 1105 28-29 6.0966431288038585 1105 30-31 5.337864004488704 1105 32-33 7.124032614837123 1105 34-35 6.154749161838261 1105 36-37 6.149861598229645 1105 38-39 4.015669622153386 1105 40-41 5.0630838191067085 1105 42-43 5.554028166550804 1105 44-45 6.065812498124448 1105 46-47 5.357664833737378 1105 48-49 4.9565716308331105 1105 50-51 5.931354052665416 1105 52-53 5.476440220559638 1105 54-55 5.231373776089221 1105 56-57 5.040713544543447 1105 58-59 1.903065176695673 1105 60-61 4.29998865930272 1105 62-63 -0.6345501457868217 1105 64-65 -0.25504955755418024 1105 66-67 1.002195067572199 1105 68-69 2.7928891735367216 1105 70-71 3.021739966233852 1105 72-73 2.773441455217956 1105 74-75 2.8200227882863906 1105 76-77 1.958708320951473 1105 78-79 3.3094160333132656 1105 80-81 1.8263622209117045 1105 82-83 1.097096382739707 1105 84-85 1.5029754557782447 1105 86-87 -0.16434582640904694 1105 88-89 1.711868224686718 1105 90-91 1.54990403595529 1105 92-93 1.6370259493854782 1105 94-95 1.190327118963296 1105 96-97 2.2631544788338296 1105 98-99 1.9940796125888482 1105 100 2.122685551194831 1106 1 6.16511856820869 1106 2 10.258023942647839 1106 3 6.826045512640491 1106 4 11.605931900598996 1106 5 10.005399514703765 1106 6 9.417148410137166 1106 7 6.616870240635954 1106 8 7.6376782443645155 1106 9 6.941670233725048 1106 10-11 5.8733019304733 1106 12-13 8.018738569568331 1106 14-15 7.985118021686304 1106 16-17 7.725452531785361 1106 18-19 7.2783283777535495 1106 20-21 8.182436936949859 1106 22-23 6.293718205567732 1106 24-25 5.261643801965636 1106 26-27 7.662382382536922 1106 28-29 6.5221955042053175 1106 30-31 5.7039801137601 1106 32-33 7.452043748632608 1106 34-35 6.860617577264069 1106 36-37 6.170274551550843 1106 38-39 7.088803126759192 1106 40-41 6.791852003238713 1106 42-43 6.2118417943251565 1106 44-45 4.69072482681128 1106 46-47 6.016522539750039 1106 48-49 6.073196456223112 1106 50-51 6.809242050459929 1106 52-53 5.810756471147098 1106 54-55 5.358740630200465 1106 56-57 5.144763465365209 1106 58-59 5.388509785032017 1106 60-61 5.189566925282993 1106 62-63 5.050121511442494 1106 64-65 5.286856461837164 1106 66-67 3.2987413442412645 1106 68-69 3.7360661691919574 1106 70-71 3.171143443129978 1106 72-73 3.155389033687989 1106 74-75 3.108831684903162 1106 76-77 3.9211440259417074 1106 78-79 4.371791716964992 1106 80-81 3.7549183766806458 1106 82-83 2.2175453705715498 1106 84-85 3.1847392799515397 1106 86-87 3.623023806266872 1106 88-89 3.6464024189409514 1106 90-91 3.4051939455325453 1106 92-93 3.22060045344967 1106 94-95 1.8895692003253117 1106 96-97 1.9038895937046867 1106 98-99 2.2204307710658 1106 100 3.1409241148505522 1107 1 5.96340461034902 1107 2 10.158635259993364 1107 3 5.828044844697882 1107 4 11.17927371166543 1107 5 9.336523927577932 1107 6 7.767318285925533 1107 7 3.652567812493306 1107 8 6.205928812757982 1107 9 5.472122636391678 1107 10-11 4.469060066742159 1107 12-13 6.019250123690952 1107 14-15 6.270120569287922 1107 16-17 6.9602393360653245 1107 18-19 6.107434030431957 1107 20-21 5.386093922730385 1107 22-23 5.722386537809232 1107 24-25 4.440996400733365 1107 26-27 7.0644767144444565 1107 28-29 6.208872082969027 1107 30-31 4.916982997473863 1107 32-33 4.972735144161678 1107 34-35 6.609473243857948 1107 36-37 6.40130883498972 1107 38-39 6.390043905083591 1107 40-41 7.482073922888645 1107 42-43 6.156036420384343 1107 44-45 4.385494001259907 1107 46-47 3.6615665447510466 1107 48-49 4.181451595313597 1107 50-51 4.78695551378517 1107 52-53 3.9328135631133776 1107 54-55 5.25758677004881 1107 56-57 5.1264705083062125 1107 58-59 2.861180442227603 1107 60-61 2.8244220759340237 1107 62-63 0.62998443334887 1107 64-65 2.111437285033613 1107 66-67 4.1509277858626525 1107 68-69 4.947406582288771 1107 70-71 4.295054557523713 1107 72-73 2.41021973592688 1107 74-75 2.4748054146347407 1107 76-77 3.096477729640654 1107 78-79 3.7854888221188574 1107 80-81 2.347384808282893 1107 82-83 1.6152294080277159 1107 84-85 2.3739631392040437 1107 86-87 2.385712521462139 1107 88-89 2.600803935941375 1107 90-91 1.423260669145602 1107 92-93 2.4328856735067887 1107 94-95 2.399763888468904 1107 96-97 2.461196714572324 1107 98-99 1.9764085898050183 1107 100 0.21732304785505363 1108 1 4.308071754228759 1108 2 -3.0786913060524164 1108 3 0.15407910250463175 1108 4 6.431998649094673 1108 5 6.886484075209378 1108 6 6.970173822358721 1108 7 4.669306441876433 1108 8 4.989044988758227 1108 9 4.951601204955519 1108 10-11 3.3776877237782585 1108 12-13 2.4099626180430853 1108 14-15 4.145499420625896 1108 16-17 4.673904827467876 1108 18-19 3.8777761896688183 1108 20-21 4.784863829876908 1108 22-23 4.0826589300174625 1108 24-25 3.106219053631925 1108 26-27 4.796829032227752 1108 28-29 6.153711455078454 1108 30-31 4.5346943927129 1108 32-33 4.704603902950776 1108 34-35 2.936916612880726 1108 36-37 1.2619588093522118 1108 38-39 2.600015437292935 1108 40-41 5.4337345228571685 1108 42-43 3.067371563573648 1108 44-45 3.8615768688028176 1108 46-47 4.710970472620797 1108 48-49 4.812054089513428 1108 50-51 2.289831166780189 1108 52-53 0.5035616707604618 1108 54-55 2.650198280529729 1108 56-57 2.805229322787092 1108 58-59 3.566594018431932 1108 60-61 4.670282887094718 1108 62-63 3.771608201606078 1108 64-65 4.177336476309751 1108 66-67 3.5664115733099173 1108 68-69 3.839886732255522 1108 70-71 2.5051841031051794 1108 72-73 1.6795804988585914 1108 74-75 1.3081612759789571 1108 76-77 2.4983250899923704 1108 78-79 2.9874172198505704 1108 80-81 3.0486947962510307 1108 82-83 2.200891693472208 1108 84-85 0.030921819659123884 1108 86-87 0.31563232174339007 1108 88-89 0.39249439980263645 1108 90-91 0.042989431894550734 1108 92-93 -0.07707614944716212 1108 94-95 0.9069068543989331 1108 96-97 1.7851736255822388 1108 98-99 -0.5158808018498462 1108 100 0.7006205902077127 1109 1 3.3798845266141164 1109 2 7.770537095142046 1109 3 3.74152548321187 1109 4 -3.5273195278374274 1109 5 0.4119069979578569 1109 6 4.103287673004086 1109 7 1.676276162733629 1109 8 3.5811462830480956 1109 9 2.1339238473299034 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42-43 -22.900229983815134 2213 44-45 -16.912304958929024 2213 46-47 -16.76785175774191 2213 48-49 -6.234683294835083 2213 50-51 -23.047854197500918 2213 52-53 -17.429879539821343 2213 54-55 -17.45379469806539 2213 56-57 -17.259213376362958 2213 58-59 -17.7277987402751 2213 60-61 -18.81952507667411 2213 62-63 -17.252878460907993 2213 64-65 -17.346306125724645 2213 66-67 -8.453936270831807 2213 68-69 -22.30308554369548 2213 70-71 -16.99432628005029 2213 72-73 -9.039090199723205 2213 74-75 -4.3258260201149845 2213 76-77 -3.2572791342535687 2213 78-79 -4.638871498721656 2213 80-81 -3.4845690974880945 2213 82-83 -3.5329320644190325 2213 84-85 -3.2233025213061524 2213 86-87 -3.282531341892451 2213 88-89 -2.954315987581449 2213 90-91 -3.0347222163301204 2213 92-93 -4.141467297478695 2213 94-95 -3.945214129662027 2213 96-97 -3.4703592110548804 2213 98-99 -3.9465062998407774 2213 100 -3.220919795223608 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 429.0 3 622.0 4 5061.0 5 13229.0 6 13183.0 7 15809.0 8 15291.0 9 15674.0 10 17904.0 11 21766.0 12 26695.0 13 34335.0 14 28236.0 15 30638.0 16 33625.0 17 34041.0 18 33493.0 19 33078.0 20 32513.0 21 31832.0 22 32973.0 23 35356.0 24 37460.0 25 39860.0 26 42597.0 27 45015.0 28 47304.0 29 48384.0 30 50351.0 31 54906.0 32 61962.0 33 74320.0 34 88937.0 35 103766.0 36 114055.0 37 98386.0 38 29749.0 39 157.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.51939077932524 22.41436352881567 14.379492747978867 22.686752943880222 2 15.318886845483881 22.390032039048315 39.98201418045965 22.309066935008154 3 17.68407699603056 28.90447339903477 30.756403184570736 22.655046420363938 4 13.113726808821196 19.2567741836877 38.467314074139175 29.16218493335193 5 13.078877033792752 39.07394404809252 34.429343245132074 13.417835672982656 6 28.890071432299035 39.18307511540341 18.42176842777779 13.505085024519763 7 24.358866722073007 34.78645322644377 23.761001997247142 17.09367805423608 8 23.689173602059807 36.400368915798325 22.11831388924248 17.792143592899386 9 24.48482998333584 18.417119701126925 22.82300795122714 34.275042364310096 10-11 23.17614474734525 28.965154144795726 28.04271939478968 19.81598171306935 12-13 23.311888767516177 27.492490310748902 28.582785935352728 20.612834986382193 14-15 21.686147428646848 28.346772398911575 26.125873449737025 23.841206722704552 16-17 20.512311572508164 31.700295070746066 27.472797683833388 20.314595672912382 18-19 20.47484895797977 30.53428521862437 29.148281402031994 19.842584421363867 20-21 22.59655433876309 29.329598278595657 27.91638606855935 20.157461314081893 22-23 21.246530311912995 29.362646713966097 28.497019358466634 20.893803615654274 24-25 21.53132851228788 28.84779288444549 28.222364972773136 21.398513630493497 26-27 21.45197312689504 29.087784977353042 28.398629013233734 21.061612882518187 28-29 21.275200323667143 28.6431859816566 28.993448678679368 21.08816501599689 30-31 22.09617762175006 29.062672246381954 27.898625249700064 20.94252488216792 32-33 20.853695990060103 29.141107518330774 28.720250085537756 21.28494640607137 34-35 21.164111347928415 28.191802232102432 28.589728858982777 22.054357560986375 36-37 22.008667051852065 28.79871983543784 28.144107519217116 21.048505593492976 38-39 21.43685299895494 28.255874406465065 28.63285514474691 21.674417449833083 40-41 21.84921618558484 28.503657981178236 27.977065017147453 21.670060816089467 42-43 21.477483685390407 28.875366733987807 28.60151098558602 21.045638595035765 44-45 21.92731163366468 28.175292281174073 28.370398619235814 21.526997465925437 46-47 21.68515976082765 28.743557426121402 28.456445574786372 21.114837238264574 48-49 21.642480533869715 28.340319302235727 28.54288683752893 21.474313326365625 50-51 21.867574455977213 28.756890148109804 27.86234384601181 21.51319154990117 52-53 21.100537080635668 28.81864331959976 28.248638905238376 21.832180694526194 54-55 21.744355885078775 28.54887859128823 27.76018535681186 21.946580166821132 56-57 21.78746448101981 28.824512201074775 27.73945450538202 21.64856881252339 58-59 21.381866721878446 28.196207884008608 28.212611031079955 22.20931436303299 60-61 22.12040406529465 28.556729459011898 27.61297415114459 21.70989232454886 62-63 21.245036572058883 28.80232668161137 28.368376384786053 21.5842603615437 64-65 21.346219989864924 28.318266388282034 28.123558483141082 22.211955138711964 66-67 21.746189185234662 28.614841047834304 27.75584613730905 21.883123629621988 68-69 21.760013315420924 28.309647438564788 27.93812341730767 21.99221582870662 70-71 21.832563263277784 28.52793580531327 27.62406697526919 22.01543395613976 72-73 21.749447660449373 28.66545769905914 28.05017321807417 21.53492142241732 74-75 22.029207352710706 27.994931679701512 27.657616311745176 22.31824465584261 76-77 21.845788673889654 28.558096455594118 27.84815877191592 21.747956098600305 78-79 21.824247673747294 28.21703814440562 28.258270736321123 21.700443445525963 80-81 21.811298590117637 28.211048248499697 27.88621716850229 22.091435992880378 82-83 21.25936896655416 28.546401501844986 28.190788271894647 22.003441259706207 84-85 21.556779209036357 28.018034564482576 27.822536494739037 22.602649731742034 86-87 21.911863018022242 28.164761023219224 27.840010256020797 22.083365702737737 88-89 21.698028188693087 27.765059294060112 28.34399287423372 22.19291964301308 90-91 22.15634890994331 27.785404847895588 27.741102929977234 22.317143312183862 92-93 21.20315550912025 28.19529462539223 28.366847283273145 22.23470258221437 94-95 21.519398732087087 27.51799459943604 28.112811469855703 22.849795198621166 96-97 21.933206609848497 27.847520747533398 27.981188623409636 22.238084019208465 98-99 21.623575904561182 27.489792001694163 28.117684471059718 22.768947622684934 100 21.98142024877972 27.183890550919365 27.78012211550237 23.054567084798546 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 511.0 1 585.0 2 565.5 3 441.0 4 414.5 5 498.5 6 717.0 7 1055.5 8 1515.5 9 2053.5 10 2665.0 11 3327.0 12 3895.5 13 4343.5 14 4810.0 15 5356.5 16 5799.5 17 6176.0 18 6669.5 19 7284.0 20 7949.5 21 8738.0 22 9602.0 23 10544.0 24 11676.5 25 13119.5 26 14874.0 27 16897.0 28 19666.0 29 22801.5 30 26093.0 31 29654.0 32 33455.5 33 37143.5 34 40472.0 35 43768.0 36 46913.0 37 50079.5 38 53034.0 39 55059.5 40 55899.5 41 55828.0 42 55037.5 43 53676.0 44 51619.5 45 49072.0 46 46318.5 47 44289.0 48 43218.5 49 43292.0 50 44319.5 51 42414.0 52 37426.5 53 32849.0 54 28933.5 55 25101.5 56 21157.0 57 17483.5 58 14881.0 59 12716.0 60 10805.5 61 9043.5 62 7489.5 63 6251.0 64 5046.0 65 4038.0 66 3237.0 67 2611.5 68 2032.5 69 1580.0 70 1250.0 71 1016.5 72 822.5 73 619.5 74 472.0 75 341.5 76 249.0 77 177.0 78 125.5 79 91.5 80 61.5 81 47.5 82 26.5 83 14.5 84 10.5 85 7.5 86 6.5 87 3.5 88 1.5 89 3.0 90 3.0 91 2.0 92 1.0 93 1.0 94 1.5 95 0.5 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 10.139973055983678 2 28.10188829875703 3 7.7842427400844905 4 27.453374654883746 5 15.316370430328096 6 12.356132258529499 7 10.02812212403118 8 11.260145586392717 9 6.4719000521139405 10-11 8.321598456540299 12-13 12.482640236397707 14-15 9.690005211394103 16-17 10.095412864381785 18-19 8.122151751361061 20-21 10.111733121181544 22-23 7.3247807333651185 24-25 6.574880526018162 26-27 12.016386785235122 28-29 8.01823572133456 30-31 5.3259477530020956 32-33 10.982146817168772 34-35 8.926244913346713 36-37 8.903271813010745 38-39 7.891866344373358 40-41 10.45172114606318 42-43 8.280364686706509 44-45 8.413421557430672 46-47 7.627346513355583 48-49 5.115690177076519 50-51 8.422777118653464 52-53 5.8068235998536375 54-55 6.53101333895129 56-57 6.299272622440041 58-59 4.941399536518567 60-61 5.505921030747225 62-63 3.686888076995576 64-65 5.503218313060641 66-67 3.6161323139698625 68-69 6.734895273154668 70-71 3.498148291882422 72-73 2.1513286282945434 74-75 1.1696877044363378 76-77 1.6091218800935831 78-79 2.3501516293922626 80-81 2.7795718895184454 82-83 1.3635557231086521 84-85 1.8972385155288456 86-87 0.5362122589730227 88-89 0.801598345659574 90-91 0.35558062691962256 92-93 0.6055820129286926 94-95 0.8465396897557298 96-97 0.871869005510772 98-99 0.5180555401554549 100 0.9713844567398849 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1442992.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 90.26339938118417 #Duplication Level Percentage of deduplicated Percentage of total 1 94.69629319112913 85.476093322286 2 3.7347032714914588 6.742140259296973 3 0.8372462003233586 2.2671806448049874 4 0.2907588244878298 1.0497951959339447 5 0.13942929730383666 0.6292681173987037 6 0.07812032150142235 0.4230843467681637 7 0.04810698228264861 0.3039609828361584 8 0.03403925836401357 0.24579993378802162 9 0.026996694211900804 0.21931320524584552 >10 0.10783589326420566 1.5531500978275503 >50 0.0028196533686867115 0.17542898746787072 >100 0.0032620914531242906 0.5975018321394244 >500 2.329924910126196E-4 0.11258431665385668 >1k 1.553283273417464E-4 0.20469875755246672 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1606 0.11129652832448135 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 6.930045350216772E-5 0.0 5 0.0 0.0 0.0 6.930045350216772E-5 0.0 6 0.0 0.0 0.0 6.930045350216772E-5 0.0 7 0.0 0.0 0.0 6.930045350216772E-5 0.0 8 0.0 0.0 0.0 6.930045350216772E-5 0.0 9 0.0 0.0 0.0 1.3860090700433544E-4 0.0 10-11 0.0 0.0 0.0 1.3860090700433544E-4 0.0 12-13 0.0 0.0 0.0 1.732511337554193E-4 0.0 14-15 0.0 0.0 0.0 2.0790136050650315E-4 0.0 16-17 0.0 0.0 0.0 2.0790136050650315E-4 0.0 18-19 0.0 0.0 0.0 2.0790136050650315E-4 0.0 20-21 0.0 0.0 0.0 2.0790136050650315E-4 0.0 22-23 0.0 0.0 0.0 2.772018140086709E-4 0.0 24-25 0.0 0.0 0.0 4.5045294776409014E-4 0.0 26-27 0.0 0.0 0.0 7.623049885238449E-4 0.0 28-29 0.0 0.0 0.0 0.0011088072560346835 0.0 30-31 0.0 0.0 0.0 0.005301484692915831 0.0 32-33 0.0 0.0 3.465022675108386E-5 0.015765853171743158 0.0 34-35 0.0 0.0 6.930045350216772E-5 0.033368168361293755 0.0 36-37 6.930045350216772E-5 0.0 6.930045350216772E-5 0.06122695066916518 0.0 38-39 6.930045350216772E-5 0.0 6.930045350216772E-5 0.10495553682903301 0.0 40-41 6.930045350216772E-5 0.0 6.930045350216772E-5 0.1558567199263752 0.0 42-43 6.930045350216772E-5 0.0 6.930045350216772E-5 0.20582234690143814 0.0 44-45 1.3860090700433544E-4 0.0 6.930045350216772E-5 0.27103407364697796 0.0 46-47 1.3860090700433544E-4 0.0 6.930045350216772E-5 0.3456013616153104 0.0 48-49 1.3860090700433544E-4 0.0 6.930045350216772E-5 0.41978749708938096 0.0 50-51 1.3860090700433544E-4 6.930045350216772E-5 6.930045350216772E-5 0.5012501801811791 0.0 52-53 1.3860090700433544E-4 6.930045350216772E-5 6.930045350216772E-5 0.5837177198487586 0.0 54-55 1.3860090700433544E-4 6.930045350216772E-5 6.930045350216772E-5 0.6804265027110337 0.0 56-57 1.3860090700433544E-4 6.930045350216772E-5 6.930045350216772E-5 0.7965047623271646 0.0 58-59 1.3860090700433544E-4 6.930045350216772E-5 6.930045350216772E-5 0.924745251532926 0.0 60-61 1.3860090700433544E-4 6.930045350216772E-5 6.930045350216772E-5 1.059881135862153 0.0 62-63 1.3860090700433544E-4 6.930045350216772E-5 6.930045350216772E-5 1.2009768591925667 0.0 64-65 1.3860090700433544E-4 6.930045350216772E-5 6.930045350216772E-5 1.360714404515063 0.0 66-67 1.3860090700433544E-4 6.930045350216772E-5 6.930045350216772E-5 1.553612216838347 0.0 68-69 1.3860090700433544E-4 6.930045350216772E-5 6.930045350216772E-5 1.7654983534212247 0.0 70-71 1.3860090700433544E-4 6.930045350216772E-5 6.930045350216772E-5 2.015465089203544 0.0 72-73 1.3860090700433544E-4 6.930045350216772E-5 6.930045350216772E-5 2.271253063080045 0.0 74-75 1.3860090700433544E-4 6.930045350216772E-5 6.930045350216772E-5 2.5411783294709878 0.0 76-77 1.3860090700433544E-4 6.930045350216772E-5 6.930045350216772E-5 2.851055307305931 0.0 78-79 1.3860090700433544E-4 6.930045350216772E-5 6.930045350216772E-5 3.197730825950525 0.0 80-81 1.3860090700433544E-4 6.930045350216772E-5 6.930045350216772E-5 3.5755222482175926 0.0 82-83 1.3860090700433544E-4 6.930045350216772E-5 6.930045350216772E-5 3.9847760763746436 0.0 84-85 1.3860090700433544E-4 6.930045350216772E-5 6.930045350216772E-5 4.414265636954328 0.0 86-87 1.3860090700433544E-4 6.930045350216772E-5 6.930045350216772E-5 4.883152505349996 0.0 88 1.3860090700433544E-4 6.930045350216772E-5 6.930045350216772E-5 5.275289121492011 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATACTG 500 0.0 23.33086 5 TATGTCG 185 9.202308E-5 19.191072 5 CATGGGG 2335 0.0 18.657438 4 GTACATG 8325 0.0 18.266273 1 GAGTACT 1055 0.0 17.990402 12-13 TACATGG 8400 0.0 17.79637 2 GTATCAA 6595 0.0 17.26929 1 GTACAAG 685 0.0 16.719303 1 GTCTAAC 270 2.9289396E-4 16.495693 1 ACGCGGA 220 3.6099725E-4 16.049839 7 ACTGTCG 190 0.0024022658 16.039309 8 CACACCG 225 4.1085496E-4 15.779325 5 GTATAGA 445 2.452307E-7 15.727837 1 ACATGGG 8605 0.0 15.721227 3 GGTATCA 5515 0.0 15.113397 1 GTCCAGA 550 1.0879376E-8 15.03893 1 GTCATAT 555 1.225817E-8 14.903445 1 CCCTAAG 345 1.122424E-4 14.753912 1 TATCAAC 7320 0.0 14.599603 2 GTGTTAC 355 1.4313043E-4 14.338307 1 >>END_MODULE