FastQCFastQC Report
Wed 25 May 2016
SRR1295134_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295134_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1442992
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT49910.34587856342931905No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA31770.22016754077638684No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30530.21157428454211805No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25070.17373623692993448No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA105150.022.7107241
CCCTAAG4950.020.3987661
GAGTACT50050.019.93047312-13
TATCAAC125300.019.0068872
GGTATCA78250.018.7108171
CAACGCA125900.018.333125
ATCAACG128900.018.2698633
AGTACTT53100.018.25527412-13
TCAACGC128450.018.1881224
CATGGGG32050.018.1869224
AACGCAG128600.017.8905356
GTACTTT57050.017.64866614-15
CGCAGAG140000.016.2291288
ACGCAGA140300.016.2278847
GTACTAA6850.016.2147871
CATGGGT13800.016.009744
GTATAGA4509.658834E-1015.7070491
GCAGAGT140850.015.5979269
CCTAGTA3302.1096039E-615.2899512
TACACTG6150.015.2874665
Produced by FastQC (version 0.11.5)