##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295134_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1442992 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.73688558217925 33.0 33.0 33.0 27.0 33.0 2 29.12446500049896 33.0 27.0 33.0 15.0 33.0 3 30.137198958829988 33.0 33.0 33.0 15.0 33.0 4 34.40346931930323 37.0 33.0 37.0 27.0 37.0 5 34.81924016210762 37.0 37.0 37.0 27.0 37.0 6 34.465543814518725 37.0 37.0 37.0 27.0 37.0 7 34.32684519387495 37.0 37.0 37.0 27.0 37.0 8 34.32599071928327 37.0 37.0 37.0 27.0 37.0 9 34.24024180314236 37.0 37.0 37.0 27.0 37.0 10-11 34.212158487365144 37.0 35.0 37.0 27.0 37.0 12-13 34.09128325035759 37.0 33.0 37.0 27.0 37.0 14-15 35.32703507711754 37.0 33.0 40.0 27.0 40.0 16-17 35.328281099271514 37.0 33.0 40.0 27.0 40.0 18-19 35.39314770975861 37.0 33.0 40.0 27.0 40.0 20-21 35.3926497860002 37.0 33.0 40.0 27.0 40.0 22-23 35.36260596039341 37.0 33.0 40.0 27.0 40.0 24-25 35.322401302294125 37.0 33.0 40.0 27.0 40.0 26-27 35.28119802465987 37.0 33.0 40.0 27.0 40.0 28-29 35.214099593067736 37.0 33.0 40.0 27.0 40.0 30-31 35.07308599077472 37.0 33.0 40.0 27.0 40.0 32-33 35.004957061439015 37.0 33.0 40.0 27.0 40.0 34-35 34.873554392539944 37.0 33.0 40.0 24.5 40.0 36-37 34.831654298845734 37.0 33.0 40.0 22.0 40.0 38-39 34.73362430283744 37.0 33.0 40.0 22.0 40.0 40-41 34.628584912459665 37.0 33.0 40.0 22.0 40.0 42-43 34.4707860473239 37.0 33.0 40.0 22.0 40.0 44-45 34.2809332969275 37.0 33.0 40.0 22.0 40.0 46-47 34.075630010422785 37.0 33.0 40.0 22.0 40.0 48-49 33.87872074134853 37.0 33.0 40.0 22.0 40.0 50-51 33.730069189572774 37.0 33.0 40.0 22.0 40.0 52-53 33.62256062403672 37.0 33.0 40.0 22.0 40.0 54-55 33.484318000376994 37.0 33.0 40.0 15.0 40.0 56-57 33.33502541940635 37.0 33.0 40.0 15.0 40.0 58-59 33.20230119085899 37.0 33.0 40.0 15.0 40.0 60-61 33.02549286482531 37.0 33.0 40.0 15.0 40.0 62-63 32.84109440662179 37.0 33.0 38.5 15.0 40.0 64-65 32.66786683502057 37.0 33.0 37.0 15.0 40.0 66-67 32.432987500970206 37.0 33.0 37.0 15.0 40.0 68-69 32.218404883741556 37.0 33.0 37.0 15.0 40.0 70-71 31.99739187743245 37.0 27.0 37.0 15.0 40.0 72-73 31.78493331910364 37.0 27.0 37.0 15.0 40.0 74-75 31.573734643019506 37.0 27.0 37.0 15.0 37.0 76-77 31.365322538170687 33.0 27.0 37.0 15.0 37.0 78-79 31.159823131382574 33.0 27.0 37.0 15.0 37.0 80-81 30.996378358299978 33.0 27.0 37.0 15.0 37.0 82-83 30.83175894253052 33.0 27.0 37.0 15.0 37.0 84-85 30.683895683413354 33.0 27.0 37.0 15.0 37.0 86-87 30.713793631565522 33.0 27.0 37.0 15.0 37.0 88-89 30.733424717531353 33.0 27.0 37.0 15.0 37.0 90-91 30.756864902923926 33.0 27.0 37.0 15.0 37.0 92-93 30.744909881690266 33.0 27.0 37.0 15.0 37.0 94-95 30.78433075166044 33.0 27.0 37.0 15.0 37.0 96-97 30.735141636266867 33.0 27.0 37.0 15.0 37.0 98-99 30.677522467207027 33.0 27.0 37.0 15.0 37.0 100 30.14450669165179 33.0 27.0 37.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.9421422449153418 1101 2 4.321315214697513 1101 3 2.524767421123123 1101 4 1.5376345698847018 1101 5 0.9114751018832905 1101 6 0.8963825358032551 1101 7 0.7669871042977476 1101 8 0.9097360176403839 1101 9 1.052359393636479 1101 10-11 0.9253422783855072 1101 12-13 1.0508334107810242 1101 14-15 1.2871213095695495 1101 16-17 1.4202981181550314 1101 18-19 1.3315658975622 1101 20-21 1.378249776364953 1101 22-23 1.3311892956783922 1101 24-25 1.348327382644932 1101 26-27 1.295652113316926 1101 28-29 1.2916416809926687 1101 30-31 1.2320598319391394 1101 32-33 1.4861600660470415 1101 34-35 1.4388504772354551 1101 36-37 1.4110387413747887 1101 38-39 1.4276844258294474 1101 40-41 1.5043772642979647 1101 42-43 1.6515044999594437 1101 44-45 1.5661454145747058 1101 46-47 1.5380741941582983 1101 48-49 1.4658303373934416 1101 50-51 1.3530228328126057 1101 52-53 1.3826589140970142 1101 54-55 1.4920235737382583 1101 56-57 1.4444282507360953 1101 58-59 1.5116977076800069 1101 60-61 1.537243898929546 1101 62-63 1.4175407188914093 1101 64-65 1.5025195168764114 1101 66-67 1.4651362161343044 1101 68-69 1.3831012365044018 1101 70-71 1.3787409620597053 1101 72-73 1.2835638774698275 1101 74-75 1.1650799996910308 1101 76-77 1.3603814237097716 1101 78-79 1.493154783125572 1101 80-81 1.4003994773001338 1101 82-83 1.3254904265043912 1101 84-85 1.3750253125568435 1101 86-87 1.3971540669218392 1101 88-89 1.3772777021040277 1101 90-91 1.336736647212465 1101 92-93 1.4062569921146135 1101 94-95 1.2891163814437903 1101 96-97 1.3368106672249986 1101 98-99 1.2049220034673702 1101 100 1.4305575886630315 1102 1 0.5981316279553504 1102 2 4.095870704902463 1102 3 2.3891938187463744 1102 4 1.2063069974586398 1102 5 0.5885400553586848 1102 6 0.5719507090527358 1102 7 0.5231061831558179 1102 8 0.5845199836178523 1102 9 0.6008105842742495 1102 10-11 0.6157368703715136 1102 12-13 0.6667547035165526 1102 14-15 0.9507397474889885 1102 16-17 0.9706495033365314 1102 18-19 1.0358273854446907 1102 20-21 0.956118165271441 1102 22-23 0.979108507248462 1102 24-25 0.9688841698080708 1102 26-27 0.9805911864442578 1102 28-29 1.0477060915553338 1102 30-31 1.0729637816292268 1102 32-33 1.057477685073117 1102 34-35 1.1629245094181258 1102 36-37 1.018869126390399 1102 38-39 1.094062670110013 1102 40-41 1.0916938427413143 1102 42-43 1.087616113767524 1102 44-45 1.155959768583962 1102 46-47 0.9373016745908203 1102 48-49 0.8102556793530624 1102 50-51 0.9253734941104739 1102 52-53 0.972328114356408 1102 54-55 0.925462096412403 1102 56-57 0.9261369931390817 1102 58-59 0.9759748043033909 1102 60-61 0.9470167050343008 1102 62-63 1.00066480461129 1102 64-65 0.8063357409183993 1102 66-67 0.8806510783701995 1102 68-69 0.9526600445093507 1102 70-71 0.8731779917238534 1102 72-73 0.847152530593835 1102 74-75 0.7931338837791628 1102 76-77 0.7115630129359758 1102 78-79 0.5710778403274581 1102 80-81 0.6574102149074008 1102 82-83 0.8127772201523094 1102 84-85 0.8393608477029026 1102 86-87 0.976127132418231 1102 88-89 0.9154361722966122 1102 90-91 0.9725100716344919 1102 92-93 0.9720531133048027 1102 94-95 0.9452584586882047 1102 96-97 0.8111373046347055 1102 98-99 0.9240061398591131 1102 100 0.8299792357999216 1103 1 0.6064391067007904 1103 2 4.096558620029022 1103 3 2.3762789084555997 1103 4 1.1754966950615753 1103 5 0.5666363874141922 1103 6 0.4800885020669554 1103 7 0.3956154237935081 1103 8 0.4605164384552509 1103 9 0.57651005649344 1103 10-11 0.5915211321295573 1103 12-13 0.64011431627425 1103 14-15 0.7185869381348553 1103 16-17 0.7514169369485728 1103 18-19 0.8636304381287943 1103 20-21 0.838955909379095 1103 22-23 0.6432156082271732 1103 24-25 0.7684524838092699 1103 26-27 0.8072069485765994 1103 28-29 0.7430565081735665 1103 30-31 0.7826340907502001 1103 32-33 0.7382364204990566 1103 34-35 0.8607611378115863 1103 36-37 0.7018200654212094 1103 38-39 0.7752233115052505 1103 40-41 0.7598687085149081 1103 42-43 0.7513836418271751 1103 44-45 0.8147662494673327 1103 46-47 0.852471429646819 1103 48-49 0.6558506109887503 1103 50-51 0.6813070138978432 1103 52-53 0.6586292770810971 1103 54-55 0.5452845870538567 1103 56-57 0.757481060792486 1103 58-59 0.7422190519760576 1103 60-61 0.7651236502988894 1103 62-63 0.7369308842570561 1103 64-65 0.689705152763409 1103 66-67 0.5609042169163061 1103 68-69 0.690361265609063 1103 70-71 0.6495306811283363 1103 72-73 0.6382533263356542 1103 74-75 0.693441650252062 1103 76-77 0.681080136808454 1103 78-79 0.6079471285136542 1103 80-81 0.7561875983437041 1103 82-83 0.6701302862806848 1103 84-85 0.587738440464495 1103 86-87 0.6473767003233952 1103 88-89 0.6550344630437941 1103 90-91 0.5822165362946734 1103 92-93 0.636529827940663 1103 94-95 0.6552825611959712 1103 96-97 0.6153715318547874 1103 98-99 0.6293846525664613 1103 100 0.6872218562955723 1104 1 0.4429823037340199 1104 2 3.689399288521745 1104 3 2.1389137495662247 1104 4 0.9431010184887327 1104 5 0.4891866088934904 1104 6 0.46132500317878566 1104 7 0.4010854363126768 1104 8 0.32047697766601146 1104 9 0.39164973562922967 1104 10-11 0.47663136344449697 1104 12-13 0.485677046279946 1104 14-15 0.5588430904360067 1104 16-17 0.49558772248781224 1104 18-19 0.5660637549511733 1104 20-21 0.6773824192744442 1104 22-23 0.5291705581445996 1104 24-25 0.498723389575062 1104 26-27 0.5587095440278489 1104 28-29 0.583944174962781 1104 30-31 0.4200260257592987 1104 32-33 0.6380813790817825 1104 34-35 0.7700727846552482 1104 36-37 0.6100257935011442 1104 38-39 0.674105925705696 1104 40-41 0.6502125186207977 1104 42-43 0.6279781213360565 1104 44-45 0.6670386140292237 1104 46-47 0.714789981537514 1104 48-49 0.6650676843411389 1104 50-51 0.5483549496540618 1104 52-53 0.4110283701213149 1104 54-55 0.5021689461352139 1104 56-57 0.4635890897272503 1104 58-59 0.5307725267834584 1104 60-61 0.5255098884372984 1104 62-63 0.5605095333456589 1104 64-65 0.6202422829071708 1104 66-67 0.5629073425701989 1104 68-69 0.5162237462953811 1104 70-71 0.534696474369408 1104 72-73 0.504296004987939 1104 74-75 0.4356661724490536 1104 76-77 0.47191561085216804 1104 78-79 0.4838209580189279 1104 80-81 0.44541626877045815 1104 82-83 0.5369617669587292 1104 84-85 0.7238568251965489 1104 86-87 0.5727631216979496 1104 88-89 0.651604223406391 1104 90-91 0.5600319004676351 1104 92-93 0.497190447420671 1104 94-95 0.49974244607639307 1104 96-97 0.6444217893902255 1104 98-99 0.8165588321118697 1104 100 0.5852092141841254 1105 1 0.6223246570895924 1105 2 3.8282443204878014 1105 3 2.275371169213667 1105 4 1.1748066959458114 1105 5 0.5163905631533652 1105 6 0.4953363346104993 1105 7 0.4390973018549502 1105 8 0.3408945047801808 1105 9 0.5440475927408457 1105 10-11 0.4940357703170193 1105 12-13 0.5541382783716955 1105 14-15 0.7110759948693541 1105 16-17 0.5543054657786257 1105 18-19 0.614794321499744 1105 20-21 0.5029912937975993 1105 22-23 0.49310704503241 1105 24-25 0.6090537093277106 1105 26-27 0.5417418646231056 1105 28-29 0.528152413618109 1105 30-31 0.5233827494514784 1105 32-33 0.5617641174113501 1105 34-35 0.687684389588405 1105 36-37 0.6130835764358551 1105 38-39 0.5056193694065385 1105 40-41 0.5215310119685839 1105 42-43 0.4892881425853659 1105 44-45 0.5781961144839087 1105 46-47 0.539300936559421 1105 48-49 0.44935953892493785 1105 50-51 0.4234828944565052 1105 52-53 0.48823819499290977 1105 54-55 0.5260458750916044 1105 56-57 0.4989483596533688 1105 58-59 0.3846311302224876 1105 60-61 0.31891442883747345 1105 62-63 0.37553438560193086 1105 64-65 0.4466198622352522 1105 66-67 0.4078649475447875 1105 68-69 0.4626395651766515 1105 70-71 0.44743305426613134 1105 72-73 0.38102720233948517 1105 74-75 0.39426270487440007 1105 76-77 0.2551918496087957 1105 78-79 0.3689723392242179 1105 80-81 0.3358634021852893 1105 82-83 0.24554886036131407 1105 84-85 0.31872521989503966 1105 86-87 0.35067304579439096 1105 88-89 0.43031865152822846 1105 90-91 0.23362519830103068 1105 92-93 0.3556463153848597 1105 94-95 0.3111183102649697 1105 96-97 0.2883322383884206 1105 98-99 0.37549390314627473 1105 100 0.4704887128569055 1106 1 0.6298052912535645 1106 2 4.044887922896905 1106 3 2.302719300574651 1106 4 1.1566178425487479 1106 5 0.574374323745289 1106 6 0.6008526537937726 1106 7 0.41001028828014086 1106 8 0.5413906305275944 1106 9 0.5530630582752423 1106 10-11 0.5519888147447531 1106 12-13 0.5942668846718746 1106 14-15 0.6833287161804051 1106 16-17 0.6458070194955496 1106 18-19 0.6397297687129821 1106 20-21 0.6793795178931745 1106 22-23 0.6202708251394071 1106 24-25 0.48442053956066644 1106 26-27 0.5828141518051311 1106 28-29 0.5134253696093367 1106 30-31 0.4906659101556272 1106 32-33 0.5131258311779447 1106 34-35 0.7556231721646398 1106 36-37 0.5975027207464692 1106 38-39 0.5281410470951968 1106 40-41 0.5334861121000287 1106 42-43 0.7524778588570129 1106 44-45 0.5807442536596099 1106 46-47 0.5565419303738608 1106 48-49 0.6667235602026551 1106 50-51 0.5303740061971283 1106 52-53 0.4829112197970318 1106 54-55 0.6201611790133938 1106 56-57 0.6209658798061071 1106 58-59 0.6086015146303936 1106 60-61 0.5362427412176061 1106 62-63 0.668766898281838 1106 64-65 0.5110462025942297 1106 66-67 0.5172695749403502 1106 68-69 0.49007490656565267 1106 70-71 0.48329178813064644 1106 72-73 0.5059651459194292 1106 74-75 0.4448063701268197 1106 76-77 0.5754762943137308 1106 78-79 0.5203495738794359 1106 80-81 0.43384574093356676 1106 82-83 0.6250861425126146 1106 84-85 0.5817842567967659 1106 86-87 0.6458590196867355 1106 88-89 0.6497485247887802 1106 90-91 0.5378902408098156 1106 92-93 0.7494819176984464 1106 94-95 0.6132606813140633 1106 96-97 0.5312013126379647 1106 98-99 0.47993086934438267 1106 100 0.6524851332841344 1107 1 0.5287205267675574 1107 2 3.5739877417816963 1107 3 1.9840077019225255 1107 4 0.9472251137034746 1107 5 0.37965877738107423 1107 6 0.10621824532974244 1107 7 0.008877033729781658 1107 8 0.12787828001187052 1107 9 0.11640157244547567 1107 10-11 0.11805872178941001 1107 12-13 0.07852912921594424 1107 14-15 0.1799335553389696 1107 16-17 0.028851125116119647 1107 18-19 0.12036585559690138 1107 20-21 0.1943474266761953 1107 22-23 0.05363461194583152 1107 24-25 0.1768112388880141 1107 26-27 0.10818018221364412 1107 28-29 -0.02345860319341142 1107 30-31 0.05115234150096626 1107 32-33 0.10654365282870515 1107 34-35 0.3331604977395628 1107 36-37 0.22830654257705874 1107 38-39 0.09195948445203328 1107 40-41 -0.008599998975405754 1107 42-43 0.13233505401186108 1107 44-45 0.19610072520996624 1107 46-47 0.1665526666949475 1107 48-49 0.06635356923700897 1107 50-51 0.07967641293136296 1107 52-53 -0.05904060698318858 1107 54-55 -0.044862177569477524 1107 56-57 -0.03029662806149247 1107 58-59 0.02063143931558642 1107 60-61 0.1948829642076788 1107 62-63 0.20803539408365168 1107 64-65 0.10598584156268487 1107 66-67 0.16038484213880366 1107 68-69 0.10118137918642844 1107 70-71 0.04950384706373612 1107 72-73 0.11280053231994458 1107 74-75 0.0805364827779087 1107 76-77 -0.015089765638400365 1107 78-79 -0.093149021728415 1107 80-81 0.06979710957365981 1107 82-83 0.14423115456555635 1107 84-85 0.18110282688578394 1107 86-87 0.21794244400584262 1107 88-89 0.12127302886279878 1107 90-91 0.23548270376254976 1107 92-93 0.23221142663217975 1107 94-95 0.17230145578495382 1107 96-97 0.20503524968865605 1107 98-99 0.25341424356779285 1107 100 0.17711155640864717 1108 1 0.4900649984571466 1108 2 3.68499181839978 1108 3 2.10887887977578 1108 4 0.9655546648693445 1108 5 0.32954632770421455 1108 6 0.16782823025184257 1108 7 0.26225369699503176 1108 8 0.26502754109311155 1108 9 0.08469228658976391 1108 10-11 0.03925320134793964 1108 12-13 0.11587232156834659 1108 14-15 0.21781107403404576 1108 16-17 0.034347959771935166 1108 18-19 0.06969496508683903 1108 20-21 0.11389006363381071 1108 22-23 0.028536186748986836 1108 24-25 0.0929074074762255 1108 26-27 -0.03921678545263063 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2210 82-83 0.33516789629160826 2210 84-85 0.5278221285588351 2210 86-87 0.4408696751893224 2210 88-89 0.5354794195283681 2210 90-91 0.44856263733881363 2210 92-93 0.4968138185661992 2210 94-95 0.4582399246413935 2210 96-97 0.3412086861401704 2210 98-99 0.33402398430004254 2210 100 0.25877190913963943 2211 1 0.5662984427218305 2211 2 3.9469128581285204 2211 3 2.2547865675633467 2211 4 1.1692028436050563 2211 5 0.6620537968936091 2211 6 0.31636386734120947 2211 7 0.3514915979754605 2211 8 0.38545711600693267 2211 9 0.3612538164505139 2211 10-11 0.23632836785483136 2211 12-13 0.3467631875925292 2211 14-15 0.22928543771686094 2211 16-17 0.2619115130155407 2211 18-19 0.28595978026089597 2211 20-21 0.2612292806606078 2211 22-23 0.3095326164795935 2211 24-25 0.2024979471098547 2211 26-27 0.3027331893414029 2211 28-29 0.27346331728951156 2211 30-31 0.21615510801251503 2211 32-33 0.18791055870597262 2211 34-35 0.32401024485612595 2211 36-37 0.16589551179914253 2211 38-39 -0.016483455129396418 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4.216939408657371 2212 3 2.554310078207635 2212 4 1.452423856149018 2212 5 0.806617876060379 2212 6 0.6994815507443093 2212 7 0.5526271574831725 2212 8 0.6092410193509252 2212 9 0.31359957567076435 2212 10-11 0.5336157330515121 2212 12-13 0.5463961487722742 2212 14-15 0.7169908207080411 2212 16-17 0.7866288882268293 2212 18-19 0.7358690203836176 2212 20-21 0.6978565228254254 2212 22-23 0.7244009534435847 2212 24-25 0.7357703793684678 2212 26-27 0.7781160009550803 2212 28-29 0.5863932942485803 2212 30-31 0.5938347484989208 2212 32-33 0.6138180146462204 2212 34-35 0.7449636561460977 2212 36-37 0.7351572552865875 2212 38-39 0.7210217582583383 2212 40-41 0.771305282929319 2212 42-43 0.6094600647371493 2212 44-45 0.9171633323253943 2212 46-47 0.8579530985667887 2212 48-49 0.7413641020256136 2212 50-51 0.7275256890015314 2212 52-53 0.6104649624694147 2212 54-55 0.4200226602750803 2212 56-57 0.7728278150482524 2212 58-59 0.6920499611830806 2212 60-61 0.6054340133064215 2212 62-63 0.7609163180209677 2212 64-65 0.6613301454966063 2212 66-67 0.5916054575836753 2212 68-69 0.5791747262041369 2212 70-71 0.5416702574233803 2212 72-73 0.6068886953356092 2212 74-75 0.5870566364301197 2212 76-77 0.5151046866895932 2212 78-79 0.6151849991237555 2212 80-81 0.5316926155105222 2212 82-83 0.47081277790203657 2212 84-85 0.5886359432931734 2212 86-87 0.5502098481327167 2212 88-89 0.7160135372527314 2212 90-91 0.420915747127939 2212 92-93 0.6703223558716402 2212 94-95 0.8655982021158017 2212 96-97 0.5786798750513924 2212 98-99 0.7191080160771612 2212 100 0.6063248918540403 2213 1 -1.0067488098306576 2213 2 -25.29018548656476 2213 3 -14.40855917692285 2213 4 -6.761982707315951 2213 5 -2.6069954332154808 2213 6 -2.074027762054925 2213 7 -1.7307545934701025 2213 8 -1.4439438264179003 2213 9 -1.519662618867386 2213 10-11 -1.543989383011784 2213 12-13 -1.5675521408390125 2213 14-15 -1.9234445734266288 2213 16-17 -2.0106156622429054 2213 18-19 -1.8787028404034203 2213 20-21 -1.918904168532471 2213 22-23 -2.040120163042225 2213 24-25 -2.0759244061681486 2213 26-27 -1.8544328701618085 2213 28-29 -2.0710981150469863 2213 30-31 -2.0679021334969434 2213 32-33 -2.0946514964603935 2213 34-35 -2.2718996356796524 2213 36-37 -2.015783351952514 2213 38-39 -1.9319888334949624 2213 40-41 -2.0759083515053973 2213 42-43 -2.355546751615421 2213 44-45 -2.053247458698884 2213 46-47 -1.9534007149153254 2213 48-49 -2.0052633940349907 2213 50-51 -2.1654714738886085 2213 52-53 -2.094315317485936 2213 54-55 -1.8883547513146368 2213 56-57 -1.8207826033775802 2213 58-59 -1.9312920467593422 2213 60-61 -1.7495753418054747 2213 62-63 -1.5692511845008035 2213 64-65 -1.4795150945767475 2213 66-67 -1.6886868518287024 2213 68-69 -1.8471950765200589 2213 70-71 -1.5924627130300308 2213 72-73 -1.9321634147069418 2213 74-75 -1.9505599318467546 2213 76-77 -1.7710042229966696 2213 78-79 -1.9469170071138073 2213 80-81 -1.9200209234890835 2213 82-83 -1.842895719405707 2213 84-85 -1.6681231173288147 2213 86-87 -1.6514380599234286 2213 88-89 -1.8429245942327661 2213 90-91 -1.8012575049733321 2213 92-93 -1.8334172180738904 2213 94-95 -2.0352144479474212 2213 96-97 -1.8825923089796248 2213 98-99 -1.8906933244618855 2213 100 -1.9032491957523057 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 9.0 4 10.0 5 27.0 6 17.0 7 27.0 8 22.0 9 61.0 10 334.0 11 1832.0 12 5331.0 13 9784.0 14 12731.0 15 14067.0 16 14954.0 17 15129.0 18 15020.0 19 15689.0 20 17132.0 21 19175.0 22 21770.0 23 22317.0 24 20953.0 25 19720.0 26 20086.0 27 21427.0 28 24852.0 29 29036.0 30 34111.0 31 41135.0 32 51251.0 33 66430.0 34 88204.0 35 121208.0 36 180011.0 37 294750.0 38 242094.0 39 2285.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.725407914314545 22.49607027729915 10.716910195163397 29.061611613222905 2 13.06414255985441 23.517152969414152 35.80015073810333 27.618553732628104 3 15.18795668998858 28.94943284508854 29.752694401632162 26.10991606329072 4 10.844981693206838 19.460297857034444 36.96135648382051 32.7333639659382 5 11.339426895989716 38.94350173463849 34.00349565403298 15.713575715338813 6 28.176646177207815 40.109207775593624 17.654910362951178 14.059235684247383 7 23.922402438043687 35.269829209382806 23.184639104645647 17.623129247927857 8 24.2034882551999 36.09762455602985 21.09488256586496 18.60400462290529 9 25.076192879605546 16.870032557030264 23.03264299857459 35.0211315647896 10-11 22.979684442418456 28.241658245904738 28.240583623799065 20.53807368787774 12-13 23.28227021945399 26.810699959373213 29.32585181066529 20.581178010507504 14-15 21.96552791596801 27.183069638518383 27.050786023279283 23.800616422234324 16-17 20.051459682347556 30.75504588078172 28.14882059718327 21.044673839687455 18-19 20.052239664712303 29.040602198003302 31.059308933564957 19.84784920371944 20-21 21.710606804705325 27.98143849899855 30.250193478936705 20.057761217359417 22-23 20.52893761569449 28.128131181131344 30.37324112317189 20.969690080002273 24-25 20.735028937232943 27.779189105917656 30.306513901274112 21.17926805557529 26-27 20.53574522766244 28.19667949825131 30.456461560000943 20.811113714085312 28-29 20.245673474140656 27.765490099311858 31.031940878668745 20.95689554787874 30-31 21.22177501946909 28.104977467150356 29.9170323944155 20.75621511896506 32-33 19.859256002098444 28.504869628673273 30.655011575048626 20.98086279417966 34-35 20.210396391264243 27.693020445021045 30.74844259267345 21.34814057104127 36-37 21.316087477199268 27.967976403665862 29.89879662022432 20.817139498910556 38-39 20.636261066148087 27.807131956582303 30.47322986678347 21.08337711048614 40-41 20.944855095490084 27.690132050175393 29.79689839583311 21.568114458501412 42-43 20.752422053840668 28.14815020164434 30.041333798142194 21.058093946372793 44-45 21.094271415571697 27.464590089175385 29.817373086042082 21.623765409210836 46-47 21.124733929921767 28.069587505605583 29.494496308808333 21.31118225566432 48-49 21.228327866488844 28.04016397760766 29.22147374666929 21.51003440923421 50-51 21.377881966731316 28.2909630562052 28.46747507066719 21.8636799063963 52-53 20.605251245848617 28.515462802693207 28.72213953920411 22.15714641225406 54-55 21.262834074102827 28.08388625329603 28.434804428535383 22.218475244065758 56-57 21.239855043081953 28.409344917965296 28.30783903363903 22.042961005313725 58-59 20.642793995894728 27.773232153631238 29.135186631082572 22.44878721939146 60-61 21.408437232944674 28.063707228696046 28.378646247380996 22.149209290978288 62-63 20.498686863575905 28.513324401186917 28.915582898798043 22.07240583643914 64-65 20.52717310311604 27.86209143304718 28.901361687674804 22.709373776161975 66-67 21.110615507942995 28.073250841399016 28.509416286906408 22.306717363751584 68-69 20.864457864732373 27.893056387391585 28.698516281803865 22.54396946607218 70-71 20.875956530750745 27.88269375141673 28.411190205346937 22.83015951248559 72-73 20.760975839687738 28.17584772858267 28.772637837234587 22.290538594495 74-75 21.048395845456874 27.354069541148757 28.46223191450794 23.13530269888643 76-77 20.79943439678521 27.91134030979946 28.625483682589852 22.66374161082548 78-79 20.749013880870017 27.83458566361953 28.880970517273287 22.535429938237165 80-81 20.62414402075975 27.72139019345824 28.569294811726152 23.085170974055856 82-83 20.005094031666165 28.042782935340448 28.656804344134763 23.295318688858625 84-85 20.206388063342796 27.471035807753726 28.588156771202982 23.7344193577005 86-87 20.538530819006667 27.674920114786588 28.43473496752784 23.3518140986789 88-89 20.21416977504619 27.341472644180985 28.96683979335687 23.477517787415948 90-91 20.565768813997952 27.352769847009622 28.421855491335492 23.65960584765694 92-93 19.50844513408987 27.857559419833862 28.940528361879963 23.693467084196303 94-95 19.812615581964828 27.017307018616815 29.005914539427202 24.16416285999115 96-97 20.32317831044841 27.318456486418214 28.71466385027379 23.643701352859583 98-99 19.80064907209579 27.121871844644048 28.81963959381365 24.257839489446514 100 20.08757374119684 26.540240633549956 28.7392267094361 24.632958915817106 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 199.0 1 152.0 2 99.0 3 78.5 4 61.0 5 76.0 6 153.0 7 307.5 8 472.5 9 525.0 10 447.0 11 387.5 12 407.0 13 465.0 14 579.0 15 680.5 16 794.5 17 963.5 18 1124.0 19 1366.0 20 1745.0 21 2125.0 22 2602.0 23 3280.5 24 4331.0 25 5728.0 26 7599.0 27 9911.0 28 12895.5 29 16921.5 30 21176.5 31 25395.0 32 30267.5 33 35415.0 34 39883.0 35 44219.0 36 48831.5 37 53284.0 38 57329.0 39 60446.0 40 61966.0 41 62021.5 42 61378.5 43 60674.5 44 58347.0 45 54866.0 46 52208.5 47 49940.0 48 47989.5 49 48734.0 50 55342.5 51 53880.0 52 43480.5 53 37964.0 54 33889.5 55 29494.0 56 24727.5 57 19650.0 58 16439.5 59 14330.5 60 12139.5 61 10249.0 62 8809.0 63 7214.5 64 5601.0 65 4426.0 66 3566.5 67 2836.0 68 2104.5 69 1564.0 70 1177.5 71 916.0 72 773.5 73 557.0 74 366.5 75 267.5 76 181.5 77 129.5 78 90.0 79 46.0 80 14.0 81 5.0 82 4.5 83 2.5 84 0.5 85 0.0 86 0.5 87 0.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.9797698116136473 2 6.856517569050973 3 0.0 4 0.005890538547684256 5 0.16257886391608548 6 0.054539456906206 7 0.036313437635135885 8 0.04220397618282014 9 0.04158027210130063 10-11 0.04327813321210374 12-13 0.04116446938028762 14-15 0.04410973865412975 16-17 0.034892778338341444 18-19 0.028413185935888763 20-21 0.022765198975462093 22-23 0.023943306684998947 24-25 0.0208247862774014 26-27 0.013721489793429208 28-29 0.018295319724572278 30-31 0.022210795347444755 32-33 0.001212757936287935 34-35 0.003395722221606218 36-37 0.0038808253961213923 38-39 0.005405435373169082 40-41 0.0064795924024526825 42-43 0.00727654761772761 44-45 0.00727654761772761 46-47 0.01614700566600508 48-49 0.024670961446771708 50-51 0.021760342399680662 52-53 0.027789481854369257 54-55 0.024116557818754367 56-57 0.015142149090223647 58-59 0.01472634636921064 60-61 0.011988978455875016 62-63 0.01822601927107011 64-65 0.02058223469014381 66-67 0.021171288544912237 68-69 0.02772018140086709 70-71 0.028343885482386595 72-73 0.02477491212702496 74-75 0.02623022165057048 76-77 0.019715979021366715 78-79 0.01489959750296606 80-81 0.013860090700433545 82-83 0.0088011575947753 84-85 0.012196879816381519 86-87 0.009736713717054564 88-89 0.00817745351325579 90-91 0.008558606007517714 92-93 0.004192677436881147 94-95 0.0015592602037987737 96-97 7.969552152749288E-4 98-99 6.237040815195094E-4 100 0.10145586392717354 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1442992.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.4345034251748 #Duplication Level Percentage of deduplicated Percentage of total 1 80.78296007600453 54.47558797941087 2 11.724819309045616 15.813147357107843 3 3.75055621298839 7.587506873732286 4 1.5111040802399085 4.076022130989349 5 0.7213973320163168 2.4323535428383134 6 0.39241904207535744 1.5877549942160702 7 0.23557885528344694 1.1120300186457253 8 0.16566538031237996 0.8937250124886456 9 0.1252824286636025 0.7603522528346929 >10 0.5524302776297675 6.529958608135243 >50 0.02378900264398453 1.0873948565265503 >100 0.01192430732738619 1.6876838984069382 >500 9.331630962139635E-4 0.3835774017591882 >1k 0.0011405326731503997 1.572905072908257 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 4991 0.34587856342931905 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 3177 0.22016754077638684 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3053 0.21157428454211805 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2507 0.17373623692993448 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 6.930045350216772E-5 0.0 2 0.0 0.0 0.0 6.930045350216772E-5 0.0 3 0.0 0.0 0.0 6.930045350216772E-5 0.0 4 0.0 0.0 0.0 6.930045350216772E-5 0.0 5 0.0 0.0 0.0 6.930045350216772E-5 0.0 6 0.0 0.0 0.0 6.930045350216772E-5 0.0 7 0.0 0.0 0.0 1.3860090700433544E-4 0.0 8 0.0 0.0 0.0 2.0790136050650315E-4 0.0 9 0.0 0.0 0.0 2.0790136050650315E-4 0.0 10-11 0.0 0.0 0.0 2.0790136050650315E-4 0.0 12-13 0.0 0.0 0.0 2.772018140086709E-4 0.0 14-15 0.0 0.0 0.0 2.772018140086709E-4 0.0 16-17 0.0 0.0 0.0 2.772018140086709E-4 0.0 18-19 0.0 0.0 0.0 2.772018140086709E-4 0.0 20-21 0.0 0.0 0.0 4.158027210130063E-4 0.0 22-23 0.0 0.0 0.0 5.197534012662579E-4 0.0 24-25 0.0 0.0 0.0 7.276547617727611E-4 0.0 26-27 0.0 0.0 0.0 0.0010741570292835997 0.0 28-29 0.0 0.0 0.0 0.0020443633783139474 0.0 30-31 0.0 0.0 0.0 0.008073502833002538 0.0 32-33 0.0 0.0 0.0 0.024289808952509786 0.0 34-35 0.0 0.0 0.0 0.05169813831261712 0.0 36-37 0.0 0.0 0.0 0.09518417288522736 0.0 38-39 0.0 0.0 0.0 0.1648311286549059 0.0 40-41 0.0 0.0 0.0 0.24189323294931642 0.0 42-43 0.0 0.0 0.0 0.31583681683612935 0.0 44-45 0.0 0.0 0.0 0.4120258462971382 0.0 46-47 0.0 0.0 0.0 0.5262676438954617 0.0 48-49 0.0 0.0 0.0 0.6379106744874539 0.0 50-51 0.0 0.0 0.0 0.7608843292270504 0.0 52-53 0.0 0.0 0.0 0.8878774102697728 0.0 54-55 0.0 0.0 0.0 1.031398649472762 0.0 56-57 0.0 0.0 0.0 1.2012887112333264 0.0 58-59 0.0 0.0 0.0 1.3888503886369432 0.0 60-61 0.0 0.0 0.0 1.586218080211117 0.0 62-63 0.0 0.0 0.0 1.7928027321010789 0.0 64-65 0.0 0.0 0.0 2.0202814707219447 0.0 66-67 0.0 0.0 0.0 2.290761140740905 0.0 68-69 0.0 0.0 0.0 2.580818188874228 0.0 70-71 0.0 0.0 0.0 2.9166135363189816 0.0 72-73 0.0 0.0 0.0 3.2519584308159715 0.0 74-75 0.0 0.0 0.0 3.6090983179393925 0.0 76-77 0.0 0.0 0.0 4.017520540654418 0.0 78-79 0.0 0.0 0.0 4.467037932296229 0.0 80-81 0.0 0.0 0.0 4.950720447514609 0.0 82-83 0.0 0.0 0.0 5.467320678146518 0.0 84-85 0.0 0.0 0.0 6.007587013649418 0.0 86-87 0.0 0.0 0.0 6.594423253905774 0.0 88 0.0 0.0 0.0 7.083060751549558 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 10515 0.0 22.710724 1 CCCTAAG 495 0.0 20.398766 1 GAGTACT 5005 0.0 19.930473 12-13 TATCAAC 12530 0.0 19.006887 2 GGTATCA 7825 0.0 18.710817 1 CAACGCA 12590 0.0 18.33312 5 ATCAACG 12890 0.0 18.269863 3 AGTACTT 5310 0.0 18.255274 12-13 TCAACGC 12845 0.0 18.188122 4 CATGGGG 3205 0.0 18.186922 4 AACGCAG 12860 0.0 17.890535 6 GTACTTT 5705 0.0 17.648666 14-15 CGCAGAG 14000 0.0 16.229128 8 ACGCAGA 14030 0.0 16.227884 7 GTACTAA 685 0.0 16.214787 1 CATGGGT 1380 0.0 16.00974 4 GTATAGA 450 9.658834E-10 15.707049 1 GCAGAGT 14085 0.0 15.597926 9 CCTAGTA 330 2.1096039E-6 15.289951 2 TACACTG 615 0.0 15.287466 5 >>END_MODULE