FastQCFastQC Report
Wed 25 May 2016
SRR1295133_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295133_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2779469
Sequences flagged as poor quality0
Sequence length100
%GC43

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA2508.716157E-619.8748021
TCGACGC1756.993449E-419.2826884
CGTACAC1850.001017005218.2377473
TAGACCG2203.8264983E-415.9286135
GTATACA12700.015.649451
GTACATG97800.015.6224461
GTATAAG9000.015.182141
CGTATAC2605.629532E-415.1396683
TACATGG98250.014.5991762
CTTACAC13600.014.4717423
TAACGGT2350.00501853614.3594484
TATACTG12800.014.0797575
TACCGTA2150.00624572113.8935377
CCTATAC7751.8189894E-1213.7861693
GTATATA19500.013.6957771
ACATGGG99850.013.459893
TATACAG14550.013.4185085
CTATACT11850.013.2890544
GTATTAT15900.013.28111651
GTATCAA73500.013.1822671
Produced by FastQC (version 0.11.5)