##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295133_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2779469
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.58981877473719	33.0	27.0	33.0	6.0	33.0
2	22.64539125998527	27.0	6.0	33.0	2.0	33.0
3	25.05059131798196	33.0	15.0	33.0	15.0	33.0
4	25.075816999577977	33.0	6.0	37.0	2.0	37.0
5	26.93530778720684	33.0	15.0	37.0	2.0	37.0
6	27.708205775995342	33.0	15.0	37.0	6.0	37.0
7	27.72276719042378	33.0	15.0	37.0	6.0	37.0
8	28.62902194627823	33.0	22.0	37.0	6.0	37.0
9	29.573791252933564	37.0	27.0	37.0	15.0	37.0
10-11	29.064216402485513	35.0	24.5	37.0	10.5	37.0
12-13	27.89470956502843	33.0	15.0	37.0	4.0	37.0
14-15	29.671168845560068	33.0	27.0	37.0	10.5	40.0
16-17	30.06915457592799	37.0	27.0	40.0	6.0	40.0
18-19	30.222459937491656	37.0	27.0	40.0	10.5	40.0
20-21	29.726379211281003	33.0	27.0	40.0	6.0	40.0
22-23	30.488596562868665	37.0	27.0	40.0	15.0	40.0
24-25	30.61972556628622	37.0	27.0	40.0	15.0	40.0
26-27	29.168065195186564	33.0	24.5	40.0	4.0	40.0
28-29	29.884292467374166	33.0	27.0	40.0	10.5	40.0
30-31	30.586178151294366	37.0	27.0	40.0	15.0	40.0
32-33	29.384210797098294	33.0	27.0	40.0	6.0	40.0
34-35	28.995544472703237	33.0	22.0	37.0	6.0	40.0
36-37	29.31711956492409	33.0	24.5	37.0	6.0	40.0
38-39	29.423495998696154	33.0	24.5	37.0	6.0	40.0
40-41	28.445459366519287	33.0	22.0	37.0	6.0	40.0
42-43	29.09485534826976	33.0	22.0	37.0	6.0	40.0
44-45	28.936064226656242	33.0	22.0	37.0	6.0	40.0
46-47	28.63698713675166	33.0	22.0	37.0	6.0	40.0
48-49	28.58527312231221	33.0	22.0	37.0	6.0	40.0
50-51	28.536585045560862	33.0	22.0	37.0	6.0	40.0
52-53	28.726666136589397	33.0	22.0	37.0	6.0	40.0
54-55	28.702742142473976	33.0	22.0	37.0	6.0	40.0
56-57	28.701731337892237	33.0	22.0	37.0	6.0	40.0
58-59	28.546557633850206	33.0	22.0	37.0	6.0	40.0
60-61	28.504169501440742	33.0	22.0	37.0	10.5	40.0
62-63	28.44850077478828	33.0	22.0	37.0	10.5	40.0
64-65	28.022255689845796	33.0	22.0	37.0	6.0	40.0
66-67	28.223395188073695	33.0	22.0	37.0	10.5	40.0
68-69	27.80771111316586	33.0	22.0	37.0	6.0	38.5
70-71	27.935787195323996	33.0	22.0	37.0	10.5	37.0
72-73	27.987351181106895	33.0	22.0	37.0	10.5	37.0
74-75	28.092192429561187	33.0	22.0	37.0	15.0	37.0
76-77	27.840981856606426	33.0	22.0	37.0	15.0	37.0
78-79	27.390147542570183	33.0	22.0	37.0	10.5	37.0
80-81	27.210555325495626	30.0	22.0	33.0	10.5	37.0
82-83	27.306726032922114	27.0	22.0	33.0	15.0	37.0
84-85	27.2576788947817	27.0	22.0	33.0	15.0	37.0
86-87	27.055852035046982	27.0	22.0	33.0	15.0	37.0
88-89	27.004457685982466	27.0	22.0	33.0	15.0	37.0
90-91	27.148949853371274	27.0	22.0	33.0	15.0	37.0
92-93	27.06329842138912	27.0	22.0	33.0	15.0	37.0
94-95	26.92549638078352	27.0	22.0	33.0	15.0	37.0
96-97	27.0243922490231	27.0	22.0	33.0	15.0	37.0
98-99	27.094632463970637	27.0	22.0	33.0	15.0	37.0
100	26.76103888908277	27.0	22.0	33.0	15.0	37.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	7.436838327512621
1101	2	11.408783320799444
1101	3	8.621071382758142
1101	4	12.957012699458915
1101	5	10.800657747846653
1101	6	9.949644424658821
1101	7	9.544148131884
1101	8	9.15868962057872
1101	9	7.5215837599937
1101	10-11	7.8237193971922565
1101	12-13	9.259930750271579
1101	14-15	8.187061504510492
1101	16-17	8.404722508236357
1101	18-19	8.285550053217271
1101	20-21	7.334390441320721
1101	22-23	7.43172865652862
1101	24-25	7.468345646328334
1101	26-27	8.539193461023693
1101	28-29	8.296606527175406
1101	30-31	5.51961809758728
1101	32-33	7.015556998540198
1101	34-35	8.778663256218959
1101	36-37	8.355234281613235
1101	38-39	8.268540501270095
1101	40-41	9.18999720492754
1101	42-43	8.726007502875628
1101	44-45	8.658705155404451
1101	46-47	7.412832526873906
1101	48-49	5.526465811792971
1101	50-51	6.910445390123108
1101	52-53	6.0110726463186985
1101	54-55	4.237158334701984
1101	56-57	6.334224716098756
1101	58-59	6.336474692532899
1101	60-61	6.791169625341325
1101	62-63	6.36236237905624
1101	64-65	6.78672502096526
1101	66-67	5.877294125452767
1101	68-69	6.76363650966443
1101	70-71	6.230168563797715
1101	72-73	5.467420755971808
1101	74-75	4.888455128100794
1101	76-77	5.130377424231295
1101	78-79	5.181921650238937
1101	80-81	4.8854544393345165
1101	82-83	4.460534854381489
1101	84-85	4.270606999693495
1101	86-87	4.095422323781989
1101	88-89	4.043907221137303
1101	90-91	3.8248248032768473
1101	92-93	3.8707613360042465
1101	94-95	3.643244546130969
1101	96-97	3.7473609801702743
1101	98-99	3.5916051591384743
1101	100	3.802013223150148
1102	1	6.898959251130027
1102	2	10.643931514612092
1102	3	8.290683836032418
1102	4	12.725161518865718
1102	5	10.682026012168375
1102	6	9.583652340668657
1102	7	6.9227719628402085
1102	8	8.095097813719583
1102	9	6.675645620553542
1102	10-11	7.669040120632687
1102	12-13	9.313115965317998
1102	14-15	6.41646848706613
1102	16-17	7.519313019139712
1102	18-19	7.562982290883205
1102	20-21	7.194151693618075
1102	22-23	6.830004620535128
1102	24-25	7.188345593177207
1102	26-27	8.674687293274708
1102	28-29	7.894991604401504
1102	30-31	4.311231588020707
1102	32-33	4.55972934548155
1102	34-35	5.403455576441193
1102	36-37	5.537116698077895
1102	38-39	4.6996174317064
1102	40-41	6.414651367857413
1102	42-43	7.219585683322141
1102	44-45	6.741257158157783
1102	46-47	5.777728999179352
1102	48-49	4.036540318702645
1102	50-51	5.306510842931459
1102	52-53	6.104792206001672
1102	54-55	5.59529088877051
1102	56-57	6.531617601662592
1102	58-59	6.2313106969474
1102	60-61	6.227812532727249
1102	62-63	4.580532941106306
1102	64-65	6.372624530500982
1102	66-67	4.06430389884763
1102	68-69	6.289787147825805
1102	70-71	5.5745061011288435
1102	72-73	5.320684694948625
1102	74-75	4.525997470227313
1102	76-77	3.5750382600923665
1102	78-79	4.63303086751419
1102	80-81	4.755251965352674
1102	82-83	4.2145437775228345
1102	84-85	3.2072513454473324
1102	86-87	3.4198035748946687
1102	88-89	3.535716085768584
1102	90-91	3.495639033486178
1102	92-93	3.3412987255971807
1102	94-95	3.278046002866148
1102	96-97	3.5607218125409084
1102	98-99	3.33129373248628
1102	100	3.519337928979251
1103	1	6.658993307290377
1103	2	10.863392667973962
1103	3	7.967990656998161
1103	4	11.92818738787475
1103	5	10.440602830834695
1103	6	9.094365037865408
1103	7	8.07298557158694
1103	8	8.078535384288031
1103	9	7.090671199168124
1103	10-11	7.433889086264017
1103	12-13	8.84876571539322
1103	14-15	8.577062165437336
1103	16-17	8.093575732296454
1103	18-19	7.294526057233568
1103	20-21	8.321129762094884
1103	22-23	6.885398714284058
1103	24-25	5.310713398928943
1103	26-27	5.983864510280654
1103	28-29	7.0190916922051585
1103	30-31	6.167290934128392
1103	32-33	7.989775816952111
1103	34-35	7.150353350739291
1103	36-37	4.134729643943011
1103	38-39	6.348260628795593
1103	40-41	6.794572048160472
1103	42-43	7.409672479060557
1103	44-45	5.4885747981556925
1103	46-47	6.2977318117488394
1103	48-49	5.513132475121626
1103	50-51	6.380989805797583
1103	52-53	6.133993717078031
1103	54-55	4.939360055581897
1103	56-57	5.8884737913853336
1103	58-59	5.42588112570991
1103	60-61	4.946751775598919
1103	62-63	2.5689637975565596
1103	64-65	4.94731914412397
1103	66-67	4.167803154837198
1103	68-69	5.276019068583999
1103	70-71	3.639763647080617
1103	72-73	4.4593189500949215
1103	74-75	3.351255739936569
1103	76-77	3.74051209196924
1103	78-79	3.0791099461815
1103	80-81	4.218288211004769
1103	82-83	3.530632154161548
1103	84-85	3.4145448763536415
1103	86-87	2.90605059482742
1103	88-89	3.0480091678783943
1103	90-91	2.7002135011385953
1103	92-93	2.383513552195417
1103	94-95	2.8127448531726564
1103	96-97	3.0847294165074963
1103	98-99	2.817003965620323
1103	100	2.9338277933379615
1104	1	6.449301136944083
1104	2	9.76884037636821
1104	3	7.450139310370016
1104	4	12.247747335448892
1104	5	9.530418635765638
1104	6	8.251425866157234
1104	7	4.1074084327500024
1104	8	6.579971884586911
1104	9	6.616578234505372
1104	10-11	7.130407451326555
1104	12-13	7.576407258192468
1104	14-15	7.31093066351205
1104	16-17	7.216714128834592
1104	18-19	6.876222683881458
1104	20-21	7.61292446823294
1104	22-23	6.5661630525842085
1104	24-25	6.0588473594198256
1104	26-27	8.267140880439321
1104	28-29	7.384315574533318
1104	30-31	6.3350548841337435
1104	32-33	7.770385451890952
1104	34-35	8.036150884522055
1104	36-37	6.749932060130277
1104	38-39	7.415454973021433
1104	40-41	7.3340845919172075
1104	42-43	7.337875941375973
1104	44-45	6.919070333631172
1104	46-47	6.785889859327156
1104	48-49	5.577225351496217
1104	50-51	5.646930189823376
1104	52-53	5.890570579105624
1104	54-55	4.9738871746751805
1104	56-57	6.1212646978433405
1104	58-59	3.96037846918842
1104	60-61	4.354862107754556
1104	62-63	3.9231268180087717
1104	64-65	2.856955260144307
1104	66-67	3.2182755484278935
1104	68-69	4.228631350782734
1104	70-71	2.7842784681525394
1104	72-73	2.4923536530546
1104	74-75	3.2993786124732196
1104	76-77	3.954581883586677
1104	78-79	4.4977020737513165
1104	80-81	3.253460829072438
1104	82-83	3.0133179803976375
1104	84-85	3.0544703576842487
1104	86-87	0.8724442260311562
1104	88-89	0.30708129566979636
1104	90-91	2.230680490108977
1104	92-93	2.5083611902014944
1104	94-95	2.4888787804256367
1104	96-97	2.8259961161697014
1104	98-99	2.557074403991809
1104	100	0.8989646166157073
1105	1	5.896740732031464
1105	2	-2.7125409449445925
1105	3	0.43304261737566563
1105	4	8.701051570387477
1105	5	8.632936680117187
1105	6	8.054884990966293
1105	7	5.45703161737422
1105	8	6.152650747757026
1105	9	5.078364370833235
1105	10-11	5.161347308281584
1105	12-13	5.2714478917871475
1105	14-15	6.047525420747579
1105	16-17	6.290510647232221
1105	18-19	6.301711454643787
1105	20-21	7.356623163318595
1105	22-23	2.122870429224214
1105	24-25	4.366027785667068
1105	26-27	7.182753427666675
1105	28-29	6.226515907890821
1105	30-31	5.495546530574504
1105	32-33	7.318782945180679
1105	34-35	6.42367213807702
1105	36-37	6.489714738713637
1105	38-39	4.298832821111862
1105	40-41	5.608321917729455
1105	42-43	5.7783051877460885
1105	44-45	6.423942512850811
1105	46-47	5.776368413973664
1105	48-49	5.2656058520487825
1105	50-51	6.039388320670227
1105	52-53	5.618320268111621
1105	54-55	5.465774056629197
1105	56-57	5.422937601427268
1105	58-59	2.021846041506816
1105	60-61	4.570048776781022
1105	62-63	-0.6230020989570519
1105	64-65	-0.26154761173684804
1105	66-67	1.0545218687881999
1105	68-69	2.956505223702898
1105	70-71	3.0818938597849446
1105	72-73	2.8750066834871824
1105	74-75	2.791923072870077
1105	76-77	2.107099149713253
1105	78-79	3.4332329931203063
1105	80-81	1.7381654453383675
1105	82-83	1.1142023298429997
1105	84-85	1.5461367666270576
1105	86-87	-0.22505478703808635
1105	88-89	1.7779729817985839
1105	90-91	1.478765933955227
1105	92-93	1.7739873773013919
1105	94-95	1.0270673481766437
1105	96-97	2.0852994347375535
1105	98-99	2.053115404637232
1105	100	2.167222431373272
1106	1	6.28197844133517
1106	2	10.484776701125252
1106	3	6.852942929880456
1106	4	11.921892604051536
1106	5	10.106803829676704
1106	6	9.605452137296687
1106	7	6.730173685270014
1106	8	7.6544880680422125
1106	9	7.091989843064443
1106	10-11	5.922100320038073
1106	12-13	8.206893427338734
1106	14-15	8.029660839526354
1106	16-17	7.974385613946719
1106	18-19	7.445379384668463
1106	20-21	8.482425228287802
1106	22-23	6.396270743166333
1106	24-25	5.248188590872271
1106	26-27	8.071630388371112
1106	28-29	6.611825316545328
1106	30-31	5.671132672767353
1106	32-33	7.59262228856533
1106	34-35	7.013082986827921
1106	36-37	6.1655819708292725
1106	38-39	7.356892744967652
1106	40-41	6.94698531679612
1106	42-43	6.561919589139816
1106	44-45	4.758126600333952
1106	46-47	6.262271537349772
1106	48-49	6.157295358638077
1106	50-51	6.918831820953827
1106	52-53	5.94521429828524
1106	54-55	5.528195263047664
1106	56-57	5.076628090373024
1106	58-59	5.155085469808736
1106	60-61	5.200917381901554
1106	62-63	5.20998181284175
1106	64-65	5.460404161811763
1106	66-67	3.3571697685149395
1106	68-69	3.643112385025127
1106	70-71	3.305602630251041
1106	72-73	3.156017008556521
1106	74-75	3.0022126916385687
1106	76-77	3.8200688701201138
1106	78-79	4.439105739303965
1106	80-81	3.90490054129981
1106	82-83	2.3403922215965167
1106	84-85	2.9897585818843737
1106	86-87	3.539983649423746
1106	88-89	3.499318185537632
1106	90-91	3.317033256207189
1106	92-93	3.08046514990259
1106	94-95	1.6020950392250626
1106	96-97	1.5964073917718622
1106	98-99	2.0863544868620956
1106	100	2.988021416675352
1107	1	6.04102193234386
1107	2	10.380230571235057
1107	3	6.111854447998997
1107	4	11.372534743135319
1107	5	9.472349991894628
1107	6	8.01283216889005
1107	7	3.783105215078134
1107	8	6.367954121810204
1107	9	5.661277860710886
1107	10-11	4.4732431904476115
1107	12-13	6.154232059127015
1107	14-15	6.132585794016425
1107	16-17	7.009114572256632
1107	18-19	6.247518747820912
1107	20-21	5.554590057350666
1107	22-23	5.660351775783262
1107	24-25	4.512077646705446
1107	26-27	7.159782762969279
1107	28-29	6.435758442377427
1107	30-31	4.988675116363002
1107	32-33	4.909699369240478
1107	34-35	6.5875746716576735
1107	36-37	6.481178534671251
1107	38-39	6.464495588183482
1107	40-41	7.654126976644601
1107	42-43	6.157752434675661
1107	44-45	3.9656393155884864
1107	46-47	3.5878117466545483
1107	48-49	4.038847274753266
1107	50-51	4.763863841600106
1107	52-53	3.899936523754878
1107	54-55	5.31381037993437
1107	56-57	5.203338022961741
1107	58-59	2.9397425551722236
1107	60-61	2.7269994265027364
1107	62-63	0.37768811248492185
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2207	14-15	8.593280642014594
2207	16-17	7.70299951755722
2207	18-19	4.382104344120073
2207	20-21	7.7699280412575575
2207	22-23	6.606116075888288
2207	24-25	4.616447142539133
2207	26-27	4.347180444221095
2207	28-29	5.494840260596398
2207	30-31	5.88115173068007
2207	32-33	7.123999882928608
2207	34-35	6.582599865669959
2207	36-37	7.1095881856330365
2207	38-39	7.195007233007161
2207	40-41	4.3420799069554725
2207	42-43	6.073785961306125
2207	44-45	4.290911341448616
2207	46-47	5.96460170664654
2207	48-49	2.7420437880490844
2207	50-51	5.786936162514198
2207	52-53	3.1370211699148847
2207	54-55	3.4845320770259214
2207	56-57	5.733554123427208
2207	58-59	4.06571797006632
2207	60-61	3.905337275551169
2207	62-63	4.6267103477843285
2207	64-65	3.758024795396949
2207	66-67	3.8398206538924775
2207	68-69	3.8518345768829683
2207	70-71	4.1042663109733475
2207	72-73	4.229359894728308
2207	74-75	3.828190830650108
2207	76-77	4.341852385059731
2207	78-79	4.4379245035351325
2207	80-81	4.515878540430503
2207	82-83	4.2385576399119
2207	84-85	3.576155503686884
2207	86-87	3.7706505126059433
2207	88-89	3.5095322168832226
2207	90-91	1.5624021288289605
2207	92-93	-0.4985553975705841
2207	94-95	1.4409291289384996
2207	96-97	-0.8165991662517378
2207	98-99	1.0440423314892726
2207	100	2.4330843118813945
2208	1	1.143557084711862
2208	2	8.655799372391296
2208	3	6.693555872429862
2208	4	11.916591627840283
2208	5	10.213289742645092
2208	6	9.198818664340841
2208	7	7.601437422828987
2208	8	7.769941805502583
2208	9	6.629528582444561
2208	10-11	6.750446739000914
2208	12-13	7.076322717567038
2208	14-15	8.037696736975473
2208	16-17	7.24361046277966
2208	18-19	6.589806881656095
2208	20-21	6.586530469762174
2208	22-23	5.909117216331687
2208	24-25	6.252740259577383
2208	26-27	8.124447166162
2208	28-29	7.143181169402482
2208	30-31	6.000556391525134
2208	32-33	8.394071851568725
2208	34-35	6.54862017994159
2208	36-37	6.9881943250309675
2208	38-39	7.297615570182643
2208	40-41	7.719849281466129
2208	42-43	7.073504880595845
2208	44-45	6.118603945452239
2208	46-47	4.553847991630747
2208	48-49	4.672318582798955
2208	50-51	6.028758550163374
2208	52-53	5.636333078215056
2208	54-55	6.380995093086451
2208	56-57	6.451301098020185
2208	58-59	5.776999046159421
2208	60-61	5.23169510566138
2208	62-63	3.376610267115602
2208	64-65	3.43901900611316
2208	66-67	2.8493292738372453
2208	68-69	5.5107170478920295
2208	70-71	5.3384073737293924
2208	72-73	4.673743009833938
2208	74-75	2.106221115391584
2208	76-77	3.3471844097524084
2208	78-79	4.224595075016662
2208	80-81	3.594484389605711
2208	82-83	1.3657598598519165
2208	84-85	1.9411608695093356
2208	86-87	1.0362266673790863
2208	88-89	0.9116090902874134
2208	90-91	2.1437031313505983
2208	92-93	0.8893201390941101
2208	94-95	-0.9775405530103711
2208	96-97	1.5062156116256382
2208	98-99	0.2208106222818884
2208	100	1.8621470573751928
2209	1	5.311883448296101
2209	2	9.595354440821044
2209	3	5.605550629959698
2209	4	-3.479817831871106
2209	5	0.542856446710914
2209	6	4.503432807068911
2209	7	2.3441753158945744
2209	8	5.243690696678865
2209	9	5.074980529058781
2209	10-11	5.219795902069979
2209	12-13	7.3977330754552355
2209	14-15	6.819764145428092
2209	16-17	6.399379426689219
2209	18-19	5.504637111312441
2209	20-21	6.016704724813302
2209	22-23	5.55419809496582
2209	24-25	5.447767939893975
2209	26-27	7.009316199115361
2209	28-29	6.419135082071698
2209	30-31	4.2560207808477735
2209	32-33	6.324392375613975
2209	34-35	5.6724660613454
2209	36-37	6.221690519180449
2209	38-39	6.914059303703418
2209	40-41	7.4281748426104635
2209	42-43	6.998698423543569
2209	44-45	6.488413422849224
2209	46-47	6.888111542751112
2209	48-49	5.204564661523715
2209	50-51	4.779404941505138
2209	52-53	5.03496355674616
2209	54-55	5.5583705365516
2209	56-57	5.222261276309059
2209	58-59	3.404558927272724
2209	60-61	4.343890166913951
2209	62-63	4.365298682425969
2209	64-65	5.209712395006836
2209	66-67	4.70343777133105
2209	68-69	3.977347077180312
2209	70-71	3.7874303967032645
2209	72-73	0.8430076979586438
2209	74-75	2.3533012043507213
2209	76-77	3.5057167728900787
2209	78-79	3.7047748092036805
2209	80-81	3.768703655204881
2209	82-83	3.1717273156891572
2209	84-85	3.1507185284971833
2209	86-87	1.6625526748838269
2209	88-89	2.169520561039839
2209	90-91	2.4396752561631025
2209	92-93	2.4547560513221107
2209	94-95	0.8485905861567957
2209	96-97	0.20361230404964914
2209	98-99	0.3705963646489572
2209	100	0.24861560425276608
2210	1	1.934363021352702
2210	2	-17.21228846219758
2210	3	-7.753909729602137
2210	4	-6.817075988694365
2210	5	-0.7233853454868431
2210	6	2.9109298804287818
2210	7	-6.92120969449844
2210	8	-7.009904958869917
2210	9	-2.774188513526159
2210	10-11	-0.6721788897534395
2210	12-13	3.7183830984143853
2210	14-15	-4.20883781737437
2210	16-17	-0.8040266949563097
2210	18-19	-0.29044008649568553
2210	20-21	2.654822919398221
2210	22-23	2.7206958984042515
2210	24-25	2.2286851485379238
2210	26-27	4.382592849029663
2210	28-29	3.567487977194162
2210	30-31	2.8218243770614855
2210	32-33	4.753772567934977
2210	34-35	2.6346851838966465
2210	36-37	4.212471323695777
2210	38-39	3.3572924949653995
2210	40-41	4.251901707641185
2210	42-43	2.83053135611512
2210	44-45	3.6041231730819767
2210	46-47	2.954490457248564
2210	48-49	3.053350598915568
2210	50-51	2.1448755928676917
2210	52-53	2.4422379226532023
2210	54-55	3.011602064484734
2210	56-57	3.076581652050205
2210	58-59	2.2010634249585763
2210	60-61	2.946993093851763
2210	62-63	2.077789891342306
2210	64-65	2.886529628284425
2210	66-67	2.8849655928316764
2210	68-69	3.4990787104203918
2210	70-71	2.60748152825062
2210	72-73	1.2129664161460845
2210	74-75	0.5076888016480154
2210	76-77	-0.20022296697571207
2210	78-79	0.5344392930768329
2210	80-81	1.153692558903721
2210	82-83	1.0515528857048828
2210	84-85	-0.8628696216057854
2210	86-87	-0.4432716366580358
2210	88-89	-1.4212981242084801
2210	90-91	-1.4901556536219616
2210	92-93	-0.9676360760345517
2210	94-95	-0.22531323548076898
2210	96-97	0.11438624686834586
2210	98-99	-0.11867276419093287
2210	100	0.010379134707310556
2211	1	1.11811205793353
2211	2	-17.21228846219758
2211	3	-8.05301959201618
2211	4	-19.189034273746508
2211	5	-21.290476886622418
2211	6	-13.271698944367378
2211	7	-11.174498635592741
2211	8	-19.46730295989639
2211	9	-15.837953546833564
2211	10-11	-9.721468031909133
2211	12-13	-22.5821962758614
2211	14-15	-18.058199712007617
2211	16-17	-16.199201001074044
2211	18-19	-19.280149446961726
2211	20-21	-8.582685127466725
2211	22-23	-4.1044062196187845
2211	24-25	-2.248468016150756
2211	26-27	-17.6579410395153
2211	28-29	-4.026303546833553
2211	30-31	-4.631990498306909
2211	32-33	-0.9025137311420792
2211	34-35	-5.452844071417815
2211	36-37	-6.393072900110244
2211	38-39	-2.593954658869496
2211	40-41	-5.309720081769935
2211	42-43	-1.6922157862557476
2211	44-45	-0.11888261536969225
2211	46-47	0.5389889325330017
2211	48-49	0.5289844273184876
2211	50-51	-4.712752658727133
2211	52-53	-1.0934611007880868
2211	54-55	-0.8193480740536216
2211	56-57	-6.536196989938819
2211	58-59	-2.2217398911434465
2211	60-61	-1.3665030198431651
2211	62-63	-0.5871960167232544
2211	64-65	-3.5627799286573953
2211	66-67	-2.563564542567299
2211	68-69	-0.05103227017718126
2211	70-71	-0.3941039466510965
2211	72-73	-1.3041107067712083
2211	74-75	-2.0050726002084502
2211	76-77	-2.044051837275237
2211	78-79	-1.2892094949991666
2211	80-81	-1.1444382756245162
2211	82-83	-1.5930974702972414
2211	84-85	-1.8690045955295318
2211	86-87	-2.329641217480308
2211	88-89	-2.8136398897744215
2211	90-91	-2.589371646468411
2211	92-93	-2.5634823945828167
2211	94-95	-2.5769027668842632
2211	96-97	-3.343479486450171
2211	98-99	-3.1201436429174016
2211	100	-2.6244939056054974
2212	1	-21.432496770072557
2212	2	-17.21228846219758
2212	3	-20.20674866355347
2212	4	-19.189034273746508
2212	5	-21.290476886622418
2212	6	-21.99544323660482
2212	7	-22.650526032853016
2212	8	-23.185339489576755
2212	9	-24.486355373317583
2212	10-11	-11.641055605496234
2212	12-13	-22.5821962758614
2212	14-15	-24.0273777941994
2212	16-17	-24.404490194376937
2212	18-19	-19.0399493013397
2212	20-21	-24.152129571911168
2212	22-23	-19.153029442380976
2212	24-25	-25.713840923304485
2212	26-27	-17.70504477921687
2212	28-29	-18.58920934593913
2212	30-31	-6.279482187127311
2212	32-33	-17.727717677235592
2212	34-35	-14.176745462352748
2212	36-37	-18.116718182539834
2212	38-39	-24.221618281396132
2212	40-41	-10.656916669623854
2212	42-43	-14.303729286395912
2212	44-45	-17.65971957514302
2212	46-47	-17.59893408157954
2212	48-49	-18.199528798134132
2212	50-51	-9.389497630157749
2212	52-53	-2.415867373071176
2212	54-55	-24.0457559888177
2212	56-57	-23.961425300441103
2212	58-59	-13.042091651119211
2212	60-61	-6.668960109321688
2212	62-63	-3.4543396707825167
2212	64-65	-17.787090293596727
2212	66-67	-18.097874615886294
2212	68-69	-15.07151177867462
2212	70-71	-10.106833456559603
2212	72-73	-18.057941045822183
2212	74-75	-6.626492490220549
2212	76-77	-11.529453652470728
2212	78-79	-4.944529778792795
2212	80-81	-2.7609664696668084
2212	82-83	-3.309354175213212
2212	84-85	-5.427302558138162
2212	86-87	-5.20371885057677
2212	88-89	-3.4831898349309824
2212	90-91	-4.41645466231186
2212	92-93	-5.767040444903319
2212	94-95	-3.934427888565203
2212	96-97	-5.71611383405785
2212	98-99	-4.2347487889815625
2212	100	-4.27912982231873
2213	1	-21.432496770072557
2213	2	-17.21228846219758
2213	3	-20.20674866355347
2213	4	-19.189034273746508
2213	5	-21.290476886622418
2213	6	-21.99544323660482
2213	7	-10.93746761360903
2213	8	-10.724858389920398
2213	9	-4.386269462664661
2213	10-11	-18.022090589928247
2213	12-13	-16.59916362981329
2213	14-15	-24.0273777941994
2213	16-17	-18.355950675476592
2213	18-19	-18.94836074009358
2213	20-21	-18.152559125175774
2213	22-23	-20.377075451836454
2213	24-25	-7.849364978287301
2213	26-27	-23.735947127795363
2213	28-29	-24.642361385494134
2213	30-31	-19.91605171847355
2213	32-33	-23.836493944231883
2213	34-35	-18.42223594952668
2213	36-37	-24.258559962515115
2213	38-39	-11.969040961808503
2213	40-41	-17.58098689122874
2213	42-43	-23.79971198108071
2213	44-45	-17.649622153099767
2213	46-47	-17.447617439762183
2213	48-49	-6.5060604502097945
2213	50-51	-23.928125565880862
2213	52-53	-18.32679188426186
2213	54-55	-18.16280925342251
2213	56-57	-18.079337671575125
2213	58-59	-18.474159315581296
2213	60-61	-19.863078362308915
2213	62-63	-18.065938033701396
2213	64-65	-18.10860188569422
2213	66-67	-9.250487882020295
2213	68-69	-23.277630443693162
2213	70-71	-17.957860242842784
2213	72-73	-9.855648272086842
2213	74-75	-4.559033512822801
2213	76-77	-3.3489257957556795
2213	78-79	-4.9826349763342
2213	80-81	-3.565348497030783
2213	82-83	-3.7327856420871655
2213	84-85	-3.4170370846632068
2213	86-87	-3.6017769406204785
2213	88-89	-3.080647453050169
2213	90-91	-3.0258301269090246
2213	92-93	-4.021299871712539
2213	94-95	-3.955552988918189
2213	96-97	-3.4842758664341638
2213	98-99	-3.971028836471355
2213	100	-3.5191003642036236
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	749.0
3	697.0
4	4140.0
5	13570.0
6	19575.0
7	24360.0
8	22337.0
9	21957.0
10	26083.0
11	32056.0
12	38634.0
13	51816.0
14	38239.0
15	42042.0
16	48238.0
17	52128.0
18	52033.0
19	53300.0
20	53156.0
21	52152.0
22	54671.0
23	59779.0
24	66818.0
25	74606.0
26	82375.0
27	89047.0
28	96685.0
29	103049.0
30	107967.0
31	118155.0
32	135047.0
33	165884.0
34	200558.0
35	236304.0
36	256276.0
37	218132.0
38	66681.0
39	173.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.94142500855787	21.03246124504866	13.990160888062986	24.035952858330482
2	14.570575441230831	21.70439229688385	40.6592409406429	23.06579132124241
3	17.440834203766563	27.227663122769073	30.204928506797053	25.126574166667304
4	12.20209999677099	17.789950938027683	39.60890029384	30.399048771361333
5	13.269340005659757	37.510807139838334	35.75266774631962	13.467185108182294
6	30.06190011657476	38.34374326857713	18.62098897115684	12.973367643691274
7	25.53587429570472	31.715141781697003	25.315105188836363	17.43387873376192
8	23.33879059650995	37.11839979036555	22.217867953117114	17.32494166000739
9	24.122357902587545	17.482997425264845	22.536815226181805	35.857829445965805
10-11	23.02023702572699	27.711169278907892	29.25061432414497	20.017979371220147
12-13	23.652550692663663	25.31259594267055	29.60248344046973	21.432369924196056
14-15	21.306049844183512	26.629915833456998	26.682301406815835	25.381732915543658
16-17	20.999805231985132	30.37483456451749	27.55032680352053	21.075033399976846
18-19	20.886852399298743	29.370199141921177	29.08442993554865	20.658518523231425
20-21	22.333235955535457	28.748782695997928	27.967379676947036	20.95060167151958
22-23	21.14999976145835	28.909320776500785	28.64078012661791	21.29989933542296
24-25	21.526304057108792	28.498353413097476	28.07962300961005	21.895719520183686
26-27	21.539592770123587	28.692343525399732	28.156938432908866	21.61112527156782
28-29	21.257642771137387	28.428724321077336	28.712437983904373	21.601194923880904
30-31	22.093336504143828	28.852295062089294	27.782165318915546	21.272203114851333
32-33	20.94051423944944	28.78911746650415	28.706318841403338	21.564049452643076
34-35	21.16130128826514	28.147262232853205	28.400565163367293	22.290871315514362
36-37	21.85329327139131	28.868585168512396	27.9408657902392	21.337255769857094
38-39	21.424345247293505	28.476781831156416	28.31893409737286	21.77993882417722
40-41	21.76479695023523	28.55496094608973	27.758065931638647	21.922176172036394
42-43	21.330551744801504	28.719202409444268	28.491782337939142	21.458463507815086
44-45	21.885904453309596	27.999615348074343	28.285232892673058	21.829247305943007
46-47	21.67202883892535	28.6614394263465	28.442478208236615	21.22405352649153
48-49	21.43029808323664	28.33156813009885	28.58844080460287	21.649692982061637
50-51	21.937007360895578	28.388949267294073	28.06003316538561	21.614010206424737
52-53	21.09544904141421	28.56134636060203	28.47172700810138	21.871477589882385
54-55	21.686905891442105	28.29210731820276	28.085081869327055	21.935904921028083
56-57	21.8454505894506	28.453389177977595	27.982063000573852	21.719097231997953
58-59	21.395934253434103	27.92762595520653	28.568788524839768	22.1076512665196
60-61	22.0135214491252	28.390362574541662	27.931984471725112	21.66413150460803
62-63	21.24081857373533	28.576913525049136	28.555515041258023	21.626752859957513
64-65	21.40880648803033	27.931859235903993	28.413893697058533	22.245440579007145
66-67	21.635160490251902	28.3600837094725	28.115626340743212	21.889129459532388
68-69	21.90915585358148	28.15375405461047	28.171950586643668	21.76513950516438
70-71	22.14550461284637	28.246771118369114	27.73917884574108	21.868545423043432
72-73	21.60723434084951	28.496964360350884	28.379126032708047	21.51667526609156
74-75	21.95660351282441	27.889885893602507	27.981984994992402	22.17152559858068
76-77	21.939171031419487	28.469771543418688	28.052505479402956	21.53855194575887
78-79	21.792456009506548	28.096239873285107	28.365333190715585	21.74597092649276
80-81	22.084061621494268	28.112128228458307	27.930998804906004	21.872811345141418
82-83	21.353187979111013	28.4127432102814	28.52494388152654	21.70912492908105
84-85	21.72455247803796	27.83904915407845	27.998095596951643	22.438302770931944
86-87	21.922009937289687	28.339875966270856	27.997611207604262	21.740502888835195
88-89	21.65019256501672	27.78446117103431	28.445881716010508	22.11946454793846
90-91	22.129374775499958	27.931231219534713	27.902469969287676	22.036924035677654
92-93	21.462838500621128	28.263758453096628	28.476221776913146	21.797181269369094
94-95	21.531895582906298	27.688671043163094	28.282340654172888	22.497092719757728
96-97	21.824735300743413	28.073872421427094	28.20862182679807	21.892770451031428
98-99	21.615191295627785	27.78157153278708	28.372282685318208	22.23095448626693
100	21.940241243123186	27.581181475695775	27.991731201923702	22.48684607925734
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	316.0
1	362.0
2	345.5
3	283.0
4	336.5
5	455.0
6	684.5
7	1042.5
8	1551.5
9	2302.0
10	3132.5
11	4033.0
12	4942.5
13	5790.5
14	6623.0
15	7425.5
16	8270.5
17	9127.0
18	9979.5
19	10831.5
20	11779.5
21	12879.0
22	14069.5
23	15721.0
24	17846.0
25	20526.0
26	24417.5
27	29706.5
28	36128.5
29	43820.0
30	51922.5
31	60485.0
32	69320.0
33	77278.0
34	84710.5
35	91590.5
36	97537.0
37	102530.0
38	106232.0
39	108918.5
40	110391.0
41	109903.0
42	107194.5
43	103752.5
44	99388.5
45	93878.5
46	88617.5
47	83823.0
48	80267.5
49	77895.5
50	76812.0
51	72479.5
52	64339.0
53	57563.5
54	52487.5
55	48124.0
56	43932.5
57	39781.5
58	35773.0
59	31583.0
60	27330.0
61	23476.5
62	19805.0
63	16176.5
64	13059.5
65	10618.5
66	8504.5
67	6641.5
68	5127.0
69	3890.0
70	2999.5
71	2291.0
72	1703.0
73	1320.0
74	1023.0
75	731.5
76	501.0
77	358.0
78	263.0
79	180.0
80	124.0
81	81.0
82	46.5
83	38.0
84	33.0
85	21.5
86	8.5
87	7.0
88	5.5
89	5.5
90	5.0
91	2.5
92	3.5
93	2.5
94	1.5
95	1.5
96	1.0
97	0.5
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	fail
#Base	N-Count
1	8.035491671250876
2	24.668992530587676
3	6.241731784020617
4	24.233441711348462
5	12.402944591215084
6	9.973559697913522
7	7.972494026736762
8	8.559260779666907
9	5.063952862938928
10-11	6.829883693611981
12-13	9.883830328742649
14-15	7.385835208091905
16-17	8.008040384692185
18-19	6.696153114137989
20-21	8.063752464949241
22-23	5.7342427636357876
24-25	5.3398868632821594
26-27	9.944741243741161
28-29	6.444972043221204
30-31	4.309869978762131
32-33	8.836597925718905
34-35	7.286103928484182
36-37	6.930874206548085
38-39	6.17297764429105
40-41	8.785545008776856
42-43	6.518996973882421
44-45	6.652907443831897
46-47	6.054249930472332
48-49	4.0015197147368795
50-51	6.69561344271154
52-53	4.594330787643251
54-55	5.069475500536254
56-57	4.796545671133587
58-59	3.826486282092011
60-61	4.301073334511017
62-63	3.1549011699716747
64-65	4.616349381842359
66-67	2.599363403585361
68-69	5.1934919943341695
70-71	2.6110023173491053
72-73	1.6114588793758808
74-75	0.8151197225081482
76-77	1.1233260741530127
78-79	1.7831103710816705
80-81	2.1141628131128645
82-83	0.8203365462971525
84-85	1.2700987130995165
86-87	0.38518148610400044
88-89	0.6499083098246464
90-91	0.23893052953639707
92-93	0.3277064791872117
94-95	0.5809562905720481
96-97	0.5825393267562977
98-99	0.4758822638424821
100	0.41277668504307835
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2779469.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	94.82444760339067
#Duplication Level	Percentage of deduplicated	Percentage of total
1	97.01423742881977	91.99321473852021
2	2.305475674181209	4.372309145345755
3	0.3566561239678527	1.0145915981885416
4	0.12309213770248756	0.4668857584783552
5	0.06407401654279087	0.3037891612200329
6	0.035860418834714414	0.2040266644096812
7	0.024134063049166092	0.16019494379438035
8	0.01571369004550881	0.11920335827010173
9	0.011936081557302256	0.10186491061981848
>10	0.04639043207575528	0.6973526311822811
>50	0.001052554743367407	0.06622987908387963
>100	0.001148454327928045	0.24411509822091335
>500	1.5261610145575876E-4	0.10195339079303
>1k	7.630805070475839E-5	0.15426872187302149
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.59780950965814E-5	0.0
5	0.0	0.0	0.0	3.59780950965814E-5	0.0
6	0.0	0.0	0.0	3.59780950965814E-5	0.0
7	0.0	0.0	0.0	3.59780950965814E-5	0.0
8	0.0	0.0	0.0	3.59780950965814E-5	0.0
9	0.0	0.0	0.0	3.59780950965814E-5	0.0
10-11	0.0	1.79890475482907E-5	0.0	3.59780950965814E-5	0.0
12-13	0.0	3.59780950965814E-5	0.0	3.59780950965814E-5	0.0
14-15	0.0	3.59780950965814E-5	0.0	7.19561901931628E-5	0.0
16-17	0.0	3.59780950965814E-5	0.0	7.19561901931628E-5	0.0
18-19	0.0	3.59780950965814E-5	0.0	7.19561901931628E-5	0.0
20-21	0.0	3.59780950965814E-5	0.0	7.19561901931628E-5	0.0
22-23	0.0	5.3967142644872104E-5	0.0	7.19561901931628E-5	0.0
24-25	0.0	8.99452377414535E-5	0.0	8.99452377414535E-5	0.0
26-27	0.0	1.079342852897442E-4	0.0	1.6190142793461628E-4	0.0
28-29	3.59780950965814E-5	1.079342852897442E-4	0.0	3.058138083209419E-4	0.0
30-31	3.59780950965814E-5	1.079342852897442E-4	0.0	0.0012952114234769303	0.0
32-33	5.3967142644872104E-5	1.439123803863256E-4	0.0	0.0038676452228825	1.79890475482907E-5
34-35	7.19561901931628E-5	1.439123803863256E-4	0.0	0.007735290445765	3.59780950965814E-5
36-37	7.19561901931628E-5	1.439123803863256E-4	0.0	0.013869555659732129	3.59780950965814E-5
38-39	7.19561901931628E-5	1.439123803863256E-4	0.0	0.024806896569092872	3.59780950965814E-5
40-41	7.19561901931628E-5	1.6190142793461628E-4	0.0	0.03962987174888441	3.59780950965814E-5
42-43	7.19561901931628E-5	1.79890475482907E-4	0.0	0.05425496740564474	3.59780950965814E-5
44-45	7.19561901931628E-5	1.79890475482907E-4	0.0	0.07220803685883886	3.59780950965814E-5
46-47	7.19561901931628E-5	1.79890475482907E-4	0.0	0.09510809438781292	3.59780950965814E-5
48-49	7.19561901931628E-5	1.79890475482907E-4	0.0	0.11827798763001135	3.59780950965814E-5
50-51	7.19561901931628E-5	1.79890475482907E-4	0.0	0.14526155895244738	3.59780950965814E-5
52-53	7.19561901931628E-5	1.79890475482907E-4	0.0	0.175249301215448	3.59780950965814E-5
54-55	1.079342852897442E-4	1.79890475482907E-4	0.0	0.20818724727636825	3.59780950965814E-5
56-57	1.259233328380349E-4	1.79890475482907E-4	0.0	0.24765521759731804	3.59780950965814E-5
58-59	1.439123803863256E-4	1.978795230311977E-4	0.0	0.29304158456165547	3.59780950965814E-5
60-61	1.439123803863256E-4	2.158685705794884E-4	0.0	0.33993903152004934	3.59780950965814E-5
62-63	1.439123803863256E-4	2.158685705794884E-4	0.0	0.3918194446493197	3.59780950965814E-5
64-65	1.439123803863256E-4	2.158685705794884E-4	0.0	0.45265840345763886	3.59780950965814E-5
66-67	1.439123803863256E-4	2.158685705794884E-4	0.0	0.5229775903239072	3.59780950965814E-5
68-69	1.439123803863256E-4	2.158685705794884E-4	0.0	0.5995389766894323	5.3967142644872104E-5
70-71	1.439123803863256E-4	2.158685705794884E-4	0.0	0.6939275091753137	8.99452377414535E-5
72-73	1.439123803863256E-4	2.158685705794884E-4	0.0	0.7961052992496048	1.079342852897442E-4
74-75	1.439123803863256E-4	2.158685705794884E-4	0.0	0.9049570259643118	1.079342852897442E-4
76-77	1.439123803863256E-4	2.158685705794884E-4	0.0	1.0225514297874883	1.079342852897442E-4
78-79	1.439123803863256E-4	2.158685705794884E-4	0.0	1.1586565635378556	1.079342852897442E-4
80-81	1.439123803863256E-4	2.158685705794884E-4	0.0	1.3161866529182373	1.079342852897442E-4
82-83	1.439123803863256E-4	2.3385761812777907E-4	0.0	1.488197925574993	1.079342852897442E-4
84-85	1.439123803863256E-4	2.518466656760698E-4	0.0	1.6656958577339773	1.079342852897442E-4
86-87	1.439123803863256E-4	2.518466656760698E-4	0.0	1.8582866007859775	1.079342852897442E-4
88	1.439123803863256E-4	2.518466656760698E-4	0.0	2.024163608228766	1.079342852897442E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCGA	250	8.716157E-6	19.874802	1
TCGACGC	175	6.993449E-4	19.282688	4
CGTACAC	185	0.0010170052	18.237747	3
TAGACCG	220	3.8264983E-4	15.928613	5
GTATACA	1270	0.0	15.64945	1
GTACATG	9780	0.0	15.622446	1
GTATAAG	900	0.0	15.18214	1
CGTATAC	260	5.629532E-4	15.139668	3
TACATGG	9825	0.0	14.599176	2
CTTACAC	1360	0.0	14.471742	3
TAACGGT	235	0.005018536	14.359448	4
TATACTG	1280	0.0	14.079757	5
TACCGTA	215	0.006245721	13.893537	7
CCTATAC	775	1.8189894E-12	13.786169	3
GTATATA	1950	0.0	13.695777	1
ACATGGG	9985	0.0	13.45989	3
TATACAG	1455	0.0	13.418508	5
CTATACT	1185	0.0	13.289054	4
GTATTAT	1590	0.0	13.2811165	1
GTATCAA	7350	0.0	13.182267	1
>>END_MODULE