FastQCFastQC Report
Wed 25 May 2016
SRR1295133_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295133_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2779469
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT60290.21691193533728922No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA45560.16391620126002485No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAC1200.00509515319.5638457
ACCGAAC1456.6475244E-419.4289238
TAGCGCT1550.001030490318.202314
GTATCAA102600.017.3063451
GAGTACT35150.016.76393712-13
CATGGGG43950.016.5836354
GTATAGG7950.015.6407431
GTACATG131800.015.3590191
CTATACT12700.015.1805344
ATCAACG113800.015.1233
TATCAAC114950.015.0878892
CAACGCA112350.015.0673325
AACGCAG113250.015.010346
TCAACGC114700.014.963614
TATGCGA2551.1248118E-414.7521985
ACATGGG127650.014.7345963
TACATGG132900.014.583162
TGTATCG1950.00477325314.4685025
CTAAGAC10800.014.3677673
TATACAG17500.014.241145
Produced by FastQC (version 0.11.5)