##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295133_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2779469 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.474643178247355 33.0 33.0 33.0 27.0 33.0 2 30.169942532188703 33.0 33.0 33.0 27.0 33.0 3 31.013450770632808 33.0 33.0 33.0 27.0 33.0 4 35.27628550633232 37.0 37.0 37.0 33.0 37.0 5 35.53955089983015 37.0 37.0 37.0 33.0 37.0 6 35.35646736840742 37.0 37.0 37.0 33.0 37.0 7 35.27526876536489 37.0 37.0 37.0 33.0 37.0 8 35.28665151509155 37.0 37.0 37.0 33.0 37.0 9 35.26057782979411 37.0 37.0 37.0 33.0 37.0 10-11 35.23258849082325 37.0 37.0 37.0 33.0 37.0 12-13 35.14219820404545 37.0 37.0 37.0 33.0 37.0 14-15 36.65407565257968 40.0 37.0 40.0 33.0 40.0 16-17 36.643872984372194 40.0 37.0 40.0 33.0 40.0 18-19 36.66627078769362 40.0 37.0 40.0 33.0 40.0 20-21 36.66510905500296 40.0 37.0 40.0 33.0 40.0 22-23 36.630048761112285 40.0 37.0 40.0 33.0 40.0 24-25 36.60673369625637 40.0 37.0 40.0 33.0 40.0 26-27 36.58218440284817 40.0 37.0 40.0 33.0 40.0 28-29 36.53867861091453 40.0 37.0 40.0 33.0 40.0 30-31 36.457293641339405 40.0 37.0 40.0 33.0 40.0 32-33 36.41694852506 40.0 37.0 40.0 33.0 40.0 34-35 36.313042707078225 38.5 37.0 40.0 33.0 40.0 36-37 36.311748395107124 37.0 37.0 40.0 33.0 40.0 38-39 36.25079538573735 37.0 37.0 40.0 33.0 40.0 40-41 36.193226116211406 37.0 37.0 40.0 27.0 40.0 42-43 36.10585978832648 37.0 37.0 40.0 27.0 40.0 44-45 36.00773313176006 37.0 37.0 40.0 27.0 40.0 46-47 35.90028742900172 37.0 37.0 40.0 27.0 40.0 48-49 35.8026862325142 37.0 37.0 40.0 27.0 40.0 50-51 35.70330160185273 37.0 37.0 40.0 27.0 40.0 52-53 35.605438484832895 37.0 37.0 40.0 27.0 40.0 54-55 35.4973725197151 37.0 37.0 40.0 27.0 40.0 56-57 35.3660244096984 37.0 33.0 40.0 27.0 40.0 58-59 35.24144683750745 37.0 33.0 40.0 27.0 40.0 60-61 35.083798560084674 37.0 33.0 40.0 27.0 40.0 62-63 34.89498119964641 37.0 33.0 40.0 27.0 40.0 64-65 34.725240324680726 37.0 33.0 40.0 27.0 40.0 66-67 34.49726062783935 37.0 33.0 40.0 27.0 40.0 68-69 34.28509402335482 37.0 33.0 38.5 27.0 40.0 70-71 34.050631973229414 37.0 33.0 37.0 27.0 40.0 72-73 33.832347833345146 37.0 33.0 37.0 27.0 40.0 74-75 33.59804732486673 37.0 33.0 37.0 27.0 40.0 76-77 33.35943340256718 37.0 33.0 37.0 27.0 38.5 78-79 33.12986059567493 37.0 33.0 37.0 24.5 37.0 80-81 32.91370078241563 37.0 33.0 37.0 22.0 37.0 82-83 32.710768855490024 37.0 33.0 37.0 22.0 37.0 84-85 32.53726305276295 37.0 33.0 37.0 22.0 37.0 86-87 32.56334645214608 37.0 33.0 37.0 22.0 37.0 88-89 32.577706569132445 37.0 33.0 37.0 22.0 37.0 90-91 32.58074851707286 37.0 33.0 37.0 22.0 37.0 92-93 32.54255974792307 37.0 33.0 37.0 22.0 37.0 94-95 32.57758910065196 37.0 33.0 37.0 22.0 37.0 96-97 32.53606228383911 35.0 33.0 37.0 22.0 37.0 98-99 32.46725399707642 33.0 33.0 37.0 22.0 37.0 100 31.937642765578605 33.0 33.0 37.0 22.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6867042421468454 1101 2 4.196238671153878 1101 3 2.4132831119618636 1101 4 1.2320943963543485 1101 5 0.5348702366500504 1101 6 0.5114903646972309 1101 7 0.6311168609198461 1101 8 0.628521008992216 1101 9 0.6994053201961634 1101 10-11 0.7000333708884838 1101 12-13 0.7993488484516647 1101 14-15 1.0663973512881881 1101 16-17 1.0737935414403381 1101 18-19 1.1567804610128292 1101 20-21 1.023265313014008 1101 22-23 1.051626589921078 1101 24-25 1.0623686556498342 1101 26-27 1.1192653638524774 1101 28-29 1.0320468043030928 1101 30-31 0.9670732887801776 1101 32-33 1.1253278885146685 1101 34-35 1.2121582552615209 1101 36-37 1.141968171128589 1101 38-39 1.1399956294024278 1101 40-41 1.1033528381204931 1101 42-43 1.186590969078317 1101 44-45 1.2006660097802921 1101 46-47 1.2511626097110877 1101 48-49 1.1084874706330794 1101 50-51 1.1029227010910034 1101 52-53 1.1481710812930004 1101 54-55 1.1908920311010363 1101 56-57 1.1349974302486032 1101 58-59 1.1471326983674146 1101 60-61 1.107802269184866 1101 62-63 1.1492266556814599 1101 64-65 1.1326258131496942 1101 66-67 1.1896838649993953 1101 68-69 1.058853170323232 1101 70-71 1.1122455382777687 1101 72-73 1.041140360006871 1101 74-75 1.0625026675971512 1101 76-77 1.0053155097108188 1101 78-79 1.0212390051437268 1101 80-81 0.9748842448739268 1101 82-83 1.02699045963967 1101 84-85 1.055242507517157 1101 86-87 1.0542913774295357 1101 88-89 0.9590823640832369 1101 90-91 1.0604532641236304 1101 92-93 1.0931573037988969 1101 94-95 1.0365008384537688 1101 96-97 1.0692449133596895 1101 98-99 1.0547704028229106 1101 100 1.1034944560980158 1102 1 0.5596215881569577 1102 2 4.138809161181442 1102 3 2.4159704763682903 1102 4 1.1862887895030951 1102 5 0.5271659271737761 1102 6 0.6090298185809857 1102 7 0.5682472243403467 1102 8 0.5751089290798888 1102 9 0.649674174445984 1102 10-11 0.6967335220294402 1102 12-13 0.7501523372034313 1102 14-15 1.0345604064276515 1102 16-17 1.0018097071613639 1102 18-19 1.0679967361484444 1102 20-21 1.0207737995216561 1102 22-23 1.0435420992586586 1102 24-25 1.0408862336837572 1102 26-27 1.0148944432351001 1102 28-29 0.9581254473561174 1102 30-31 0.9645281858091934 1102 32-33 1.0490980469669253 1102 34-35 1.243840185157822 1102 36-37 1.0825817410695109 1102 38-39 1.01643756583249 1102 40-41 0.98900306890868 1102 42-43 1.058906241679722 1102 44-45 1.2078468629280792 1102 46-47 1.1312377538862393 1102 48-49 1.0462542502084275 1102 50-51 1.0698741709961581 1102 52-53 1.0317212812370897 1102 54-55 1.0157715054594831 1102 56-57 0.998303793681373 1102 58-59 1.020776296882275 1102 60-61 1.1215551074957162 1102 62-63 1.0944444412951597 1102 64-65 1.0628905999690517 1102 66-67 1.0865530640958667 1102 68-69 1.1069948510416197 1102 70-71 1.049343463077257 1102 72-73 1.0013717613481816 1102 74-75 0.981392073872307 1102 76-77 0.9236909528856714 1102 78-79 0.912384075314435 1102 80-81 0.9413207105743808 1102 82-83 0.9747042519330975 1102 84-85 0.8805751225615168 1102 86-87 0.8964056637324802 1102 88-89 0.8746637159529058 1102 90-91 0.8897322949496882 1102 92-93 1.0269939279992855 1102 94-95 1.0109411229251393 1102 96-97 1.0395390132950837 1102 98-99 1.0422800816690554 1102 100 1.0801440462782317 1103 1 0.5442219344573616 1103 2 4.0799290106844595 1103 3 2.312138773885099 1103 4 1.0625902505961733 1103 5 0.41818877701649626 1103 6 0.47465081447908375 1103 7 0.4768506949998397 1103 8 0.4392831900463392 1103 9 0.5179819040923164 1103 10-11 0.5805577428994937 1103 12-13 0.5956370377001647 1103 14-15 0.8048309646920515 1103 16-17 0.8308918567556276 1103 18-19 0.9080654574506113 1103 20-21 0.854064231141308 1103 22-23 0.744032184477625 1103 24-25 0.7580942949046161 1103 26-27 0.7477509045201174 1103 28-29 0.7922904192658038 1103 30-31 0.6944814686288296 1103 32-33 0.7998157731359754 1103 34-35 0.9559427056557936 1103 36-37 0.8233489719159977 1103 38-39 0.7649535286965445 1103 40-41 0.7464564059126175 1103 42-43 0.7451687257301671 1103 44-45 0.8038835051145767 1103 46-47 0.8286911917370574 1103 48-49 0.735534688737495 1103 50-51 0.6957414472460357 1103 52-53 0.7276223595800246 1103 54-55 0.7854418436770914 1103 56-57 0.6900494092515999 1103 58-59 0.6603655051734876 1103 60-61 0.596152766031615 1103 62-63 0.7257202307270845 1103 64-65 0.7093506291388678 1103 66-67 0.6168325643031594 1103 68-69 0.5814852413641916 1103 70-71 0.6252424587369987 1103 72-73 0.7028774192763194 1103 74-75 0.6601506581848398 1103 76-77 0.6106291667848112 1103 78-79 0.61688274065326 1103 80-81 0.6136833319047454 1103 82-83 0.610292332187818 1103 84-85 0.6432344292999161 1103 86-87 0.7753810855635805 1103 88-89 0.7675710235689479 1103 90-91 0.7202759443510871 1103 92-93 0.7600981048934088 1103 94-95 0.6520425609069989 1103 96-97 0.7227383567762828 1103 98-99 0.729558180216074 1103 100 0.6497694045562916 1104 1 0.5464102690293267 1104 2 3.672014513785882 1104 3 2.23632356689005 1104 4 1.0315897674348875 1104 5 0.39720856724036224 1104 6 0.40631725003850505 1104 7 0.3352962105585462 1104 8 0.37426787551284235 1104 9 0.424110470003491 1104 10-11 0.5257534734225686 1104 12-13 0.5136631689181783 1104 14-15 0.5722527288314438 1104 16-17 0.5948680456119675 1104 18-19 0.6418216477922627 1104 20-21 0.6339007387802269 1104 22-23 0.5633892856761022 1104 24-25 0.6065139108718895 1104 26-27 0.5480423073826302 1104 28-29 0.6158496040994947 1104 30-31 0.5316515716957326 1104 32-33 0.6808109503519546 1104 34-35 0.7836922889270213 1104 36-37 0.6386715205909113 1104 38-39 0.6318534578755859 1104 40-41 0.6086040950392757 1104 42-43 0.7216704424155438 1104 44-45 0.6887929205967609 1104 46-47 0.5641086276792393 1104 48-49 0.47215145874458386 1104 50-51 0.4641713842723334 1104 52-53 0.5437484603472811 1104 54-55 0.5148857200189667 1104 56-57 0.5493027614751327 1104 58-59 0.5066464270196036 1104 60-61 0.5258102564423908 1104 62-63 0.6107925897988409 1104 64-65 0.5127624558399475 1104 66-67 0.39272345675623654 1104 68-69 0.5082059819322069 1104 70-71 0.5356735577047331 1104 72-73 0.5378995233424462 1104 74-75 0.5212390625044563 1104 76-77 0.503465820358862 1104 78-79 0.5645833014908135 1104 80-81 0.5191799450151251 1104 82-83 0.5277348527005401 1104 84-85 0.5635850925603876 1104 86-87 0.5582492960958731 1104 88-89 0.5264204401486694 1104 90-91 0.5450052069770308 1104 92-93 0.6330547316901871 1104 94-95 0.5407140906932639 1104 96-97 0.5815666274760929 1104 98-99 0.5226954722866424 1104 100 0.3729381566412364 1105 1 0.45520957936317785 1105 2 3.702743282631957 1105 3 2.205115388360678 1105 4 0.9661180904778917 1105 5 0.428721885037497 1105 6 0.32014204557975035 1105 7 0.3386273888295577 1105 8 0.2081257093751816 1105 9 0.30940686644071036 1105 10-11 0.35611026228325926 1105 12-13 0.3766659755237285 1105 14-15 0.5052691874377118 1105 16-17 0.48978275403214866 1105 18-19 0.4221646754986068 1105 20-21 0.43041089213270567 1105 22-23 0.3654787778515427 1105 24-25 0.45802997252567224 1105 26-27 0.386248830617383 1105 28-29 0.39468423256376894 1105 30-31 0.36112773908300255 1105 32-33 0.4737549563578298 1105 34-35 0.5978247186761863 1105 36-37 0.4183214371706754 1105 38-39 0.4022578163512449 1105 40-41 0.31745627269321375 1105 42-43 0.42234564137330466 1105 44-45 0.5379144172235755 1105 46-47 0.4869241306945469 1105 48-49 0.4019808045484581 1105 50-51 0.3667958332017065 1105 52-53 0.3423156976419932 1105 54-55 0.32080329938181507 1105 56-57 0.30417871631313886 1105 58-59 0.33840376093809255 1105 60-61 0.37972091199976177 1105 62-63 0.4677921054512524 1105 64-65 0.3230425853539032 1105 66-67 0.23445620540726964 1105 68-69 0.25691726881338894 1105 70-71 0.2956942293235727 1105 72-73 0.3815204276953068 1105 74-75 0.29684801343726974 1105 76-77 0.3125667730015138 1105 78-79 0.31510073446651177 1105 80-81 0.33241064447997104 1105 82-83 0.3571080460527085 1105 84-85 0.3667868544732684 1105 86-87 0.29039293857972126 1105 88-89 0.3067657924231 1105 90-91 0.28493919906119203 1105 92-93 0.3738064375261061 1105 94-95 0.33262718684499504 1105 96-97 0.3162455218907141 1105 98-99 0.3641379386920818 1105 100 0.2985892862124011 1106 1 0.5589758438334229 1106 2 3.9155494676763993 1106 3 2.250795856924448 1106 4 1.0213696827742282 1106 5 0.3729537727390664 1106 6 0.3131389810395291 1106 7 0.3198517318559979 1106 8 0.2829595414548507 1106 9 0.33317889923396393 1106 10-11 0.2863316112129084 1106 12-13 0.2705533644507838 1106 14-15 0.34957326569622893 1106 16-17 0.35791822846234567 1106 18-19 0.4076544600715053 1106 20-21 0.3603179668156571 1106 22-23 0.2580030697395088 1106 24-25 0.24724971976048238 1106 26-27 0.30762902721203034 1106 28-29 0.26808747420613344 1106 30-31 0.3530333875377636 1106 32-33 0.4534659658308229 1106 34-35 0.514696201092498 1106 36-37 0.35751675945925854 1106 38-39 0.23157247410155435 1106 40-41 0.27095092668189125 1106 42-43 0.2884721664183587 1106 44-45 0.27587487797605803 1106 46-47 0.27172681835126866 1106 48-49 0.229555852962271 1106 50-51 0.2161394213191059 1106 52-53 0.20139402858269762 1106 54-55 0.24218186327423297 1106 56-57 0.2524407829384998 1106 58-59 0.22613739748702244 1106 60-61 0.28309820818960674 1106 62-63 0.2387308207590877 1106 64-65 0.17526896741610898 1106 66-67 0.14524661162665353 1106 68-69 0.14969806240770112 1106 70-71 0.20000328216062968 1106 72-73 0.25313311131883154 1106 74-75 0.23227422170180745 1106 76-77 0.2615009268463311 1106 78-79 0.14823364080960744 1106 80-81 0.25938016689726595 1106 82-83 0.317530492822506 1106 84-85 0.19917469041187275 1106 86-87 0.27485843275779587 1106 88-89 0.29118998063029977 1106 90-91 0.2784263777855145 1106 92-93 0.3233213728581248 1106 94-95 0.28187087033173697 1106 96-97 0.2054206391403639 1106 98-99 0.24089098293515576 1106 100 0.3994192822512126 1107 1 0.3975470107564334 1107 2 3.5462182988192126 1107 3 2.1152930144166504 1107 4 0.8406288442148053 1107 5 0.2835177840914227 1107 6 0.19298624046288637 1107 7 0.07267087202504285 1107 8 0.12304462321630183 1107 9 0.09099920545168771 1107 10-11 0.12413531008727574 1107 12-13 0.11958869997943822 1107 14-15 0.1358592927996014 1107 16-17 0.08268931491180354 1107 18-19 0.15959502962467553 1107 20-21 0.1336653816481359 1107 22-23 0.06439417459656482 1107 24-25 0.06070883870848576 1107 26-27 0.008331732768795064 1107 28-29 0.035475526809335634 1107 30-31 0.08429056145399016 1107 32-33 0.17800915274477802 1107 34-35 0.26372424229921876 1107 36-37 0.11112732532574654 1107 38-39 0.047059513342865955 1107 40-41 0.05172684549614104 1107 42-43 0.07269691638749975 1107 44-45 0.2114222575608764 1107 46-47 0.1351148437311167 1107 48-49 0.12918871365756246 1107 50-51 0.04403756547126392 1107 52-53 0.025942794637543898 1107 54-55 0.10552091680495579 1107 56-57 0.03437074416552832 1107 58-59 0.0831683307359583 1107 60-61 0.12423545060989483 1107 62-63 0.09196303964187535 1107 64-65 0.084123368633783 1107 66-67 0.10547880817092903 1107 68-69 0.06241206005782374 1107 70-71 0.08115834654687859 1107 72-73 0.07621277678553184 1107 74-75 0.03638624887510389 1107 76-77 0.05572064049507475 1107 78-79 0.12554352451704176 1107 80-81 0.174505558157513 1107 82-83 0.26183529866099065 1107 84-85 0.19912665162122067 1107 86-87 0.12045555753257986 1107 88-89 0.03274190442949276 1107 90-91 -0.07907135913600882 1107 92-93 0.09867951500914529 1107 94-95 0.022253973718299846 1107 96-97 0.07775316900057305 1107 98-99 0.10217714313658632 1107 100 0.03598800686619086 1108 1 0.5089301961456734 1108 2 3.697729089829412 1108 3 2.1480516018407734 1108 4 0.9803257191565322 1108 5 0.321440806598261 1108 6 0.1301237165326583 1108 7 0.08978438903087493 1108 8 0.07774271993231707 1108 9 0.12691327963402443 1108 10-11 0.1716755071082119 1108 12-13 0.1071735976635253 1108 14-15 0.05271496530847486 1108 16-17 0.007616674043710248 1108 18-19 0.0389629805070868 1108 20-21 0.09435076522867547 1108 22-23 0.061818397374040046 1108 24-25 0.13021084630945978 1108 26-27 -0.017129299745278104 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0.16648540680720458 2210 74-75 -0.47233746688360867 2210 76-77 0.043915865653225694 2210 78-79 0.19611648942893112 2210 80-81 0.1748453388276161 2210 82-83 0.25614385257271266 2210 84-85 0.21210147099107957 2210 86-87 0.3505800664261045 2210 88-89 0.37606857141068417 2210 90-91 0.3506643687141917 2210 92-93 0.3776973634190668 2210 94-95 0.3177747259037531 2210 96-97 0.23258849897924705 2210 98-99 0.2720629713080953 2210 100 0.23832437534592898 2211 1 0.5972106287485985 2211 2 4.043639697581909 2211 3 2.2027440232603794 2211 4 1.09636645869125 2211 5 0.5790816377248191 2211 6 0.3490324255387449 2211 7 0.3011499053972173 2211 8 0.2541024845492217 2211 9 0.22956614705830702 2211 10-11 0.299914180672026 2211 12-13 0.23173348103262015 2211 14-15 0.2554360681574863 2211 16-17 0.23775511005356975 2211 18-19 0.33345676460310614 2211 20-21 0.35357351331097675 2211 22-23 0.12730104870006897 2211 24-25 0.2808996189242947 2211 26-27 0.22142531362366924 2211 28-29 0.31015323944190953 2211 30-31 0.23515460447703163 2211 32-33 0.36287339830742127 2211 34-35 0.46823407378324333 2211 36-37 0.26283440166655225 2211 38-39 0.08868234681147413 2211 40-41 0.11904179004714877 2211 42-43 0.35864127240979826 2211 44-45 0.18642070828405366 2211 46-47 0.30862666886991974 2211 48-49 0.2724790602270133 2211 50-51 0.19579981118202738 2211 52-53 0.10961451270242151 2211 54-55 0.08079426969696613 2211 56-57 -0.009945752685283082 2211 58-59 0.03814105590634398 2211 60-61 0.06535235072612977 2211 62-63 0.2899445586586822 2211 64-65 0.13053781303288758 2211 66-67 0.16547852098790372 2211 68-69 0.12112587310772227 2211 70-71 0.10483677562552174 2211 72-73 0.13217719027466757 2211 74-75 0.036277043752363625 2211 76-77 0.019621167853792087 2211 78-79 0.10929293314674027 2211 80-81 0.07702751632249516 2211 82-83 -0.40096384402211527 2211 84-85 0.036926247751210894 2211 86-87 0.1911131613076833 2211 88-89 0.204908314898681 2211 90-91 0.28290659125388373 2211 92-93 0.2984706649353157 2211 94-95 0.258444815905186 2211 96-97 0.07254759885913842 2211 98-99 0.14285567348503037 2211 100 0.08035740585258466 2212 1 0.502274568859189 2212 2 4.083531478215683 2212 3 2.344692573907306 2212 4 1.178944401281555 2212 5 0.6347646595888179 2212 6 0.5653074743145936 2212 7 0.397095818291163 2212 8 0.30604053686403176 2212 9 0.3312668580474991 2212 10-11 0.3080529750194785 2212 12-13 0.2868800625837764 2212 14-15 0.4574845128803773 2212 16-17 0.41515787348551214 2212 18-19 0.40475652585072197 2212 20-21 0.42110008368648266 2212 22-23 0.2765422243627498 2212 24-25 0.2699238760920437 2212 26-27 0.22097988707718486 2212 28-29 0.16553871449143998 2212 30-31 0.2473730262277698 2212 32-33 0.3474043505136706 2212 34-35 0.30133928475644467 2212 36-37 0.3908482037748726 2212 38-39 0.29935616852421276 2212 40-41 0.394365253443155 2212 42-43 0.2952510072610721 2212 44-45 0.426005158711412 2212 46-47 0.48893364160623776 2212 48-49 0.399055926644202 2212 50-51 0.40120682850804457 2212 52-53 0.33935654158976547 2212 54-55 0.3286017080851096 2212 56-57 0.3021877228835166 2212 58-59 0.31073645808841377 2212 60-61 0.4039922334759396 2212 62-63 0.446289511651365 2212 64-65 0.512533643802783 2212 66-67 0.34325780390290106 2212 68-69 0.3157213016296936 2212 70-71 0.4116921582784414 2212 72-73 0.3607628504560694 2212 74-75 0.2531640248798226 2212 76-77 0.40575730187121195 2212 78-79 0.4029769540455703 2212 80-81 0.48474590702978304 2212 82-83 0.5549859634143886 2212 84-85 0.5535333203910184 2212 86-87 0.3714864541528229 2212 88-89 0.6224900579604693 2212 90-91 0.450684707687536 2212 92-93 0.5899289641678749 2212 94-95 0.5451106641534906 2212 96-97 0.4026298357940732 2212 98-99 0.6126802591489735 2212 100 0.4054223619307997 2213 1 -0.5451637220179286 2213 2 -25.947988917334378 2213 3 -14.8914889789835 2213 4 -6.025769852878053 2213 5 -2.1112536099646206 2213 6 -1.2810712164452838 2213 7 -1.31645746330463 2213 8 -1.1376285170356155 2213 9 -1.141646330777526 2213 10-11 -1.0410402906047906 2213 12-13 -1.1233334822023338 2213 14-15 -1.2654443395062174 2213 16-17 -1.415248232223874 2213 18-19 -1.3422628842223858 2213 20-21 -1.3502995272200309 2213 22-23 -1.3957516431670598 2213 24-25 -1.3946101568449834 2213 26-27 -1.3526412416337479 2213 28-29 -1.4625454142322383 2213 30-31 -1.4942562602784122 2213 32-33 -1.3865774011716212 2213 34-35 -1.3984112132917161 2213 36-37 -1.4917916320072848 2213 38-39 -1.3328790694982118 2213 40-41 -1.4031282581255837 2213 42-43 -1.405093644190174 2213 44-45 -1.1342751287760038 2213 46-47 -1.2344600734295028 2213 48-49 -1.4781799803981315 2213 50-51 -1.4716985019877953 2213 52-53 -1.4659074239355334 2213 54-55 -1.3966326081765317 2213 56-57 -1.3836423821632309 2213 58-59 -1.3944838087549556 2213 60-61 -1.3575595104359834 2213 62-63 -1.3495710072532248 2213 64-65 -1.2294317455668136 2213 66-67 -1.3127358712040404 2213 68-69 -1.472992385455882 2213 70-71 -1.3553795309225123 2213 72-73 -1.3094565243607406 2213 74-75 -1.5077619730217862 2213 76-77 -1.456806361214607 2213 78-79 -1.5307375082535657 2213 80-81 -1.5114428033122032 2213 82-83 -1.4015856174690526 2213 84-85 -1.391508020792152 2213 86-87 -1.3431030118630929 2213 88-89 -1.3173990334042927 2213 90-91 -1.298857597733864 2213 92-93 -1.3020516639887276 2213 94-95 -1.3516855799899332 2213 96-97 -1.3786951634625666 2213 98-99 -1.3192190883973183 2213 100 -1.2695088245919521 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 11.0 4 30.0 5 52.0 6 44.0 7 47.0 8 40.0 9 102.0 10 365.0 11 1475.0 12 4474.0 13 8146.0 14 10997.0 15 12390.0 16 13355.0 17 13815.0 18 14439.0 19 15377.0 20 17075.0 21 19281.0 22 21487.0 23 22857.0 24 23855.0 25 25629.0 26 27952.0 27 32190.0 28 37962.0 29 45832.0 30 55264.0 31 68683.0 32 87478.0 33 117862.0 34 165017.0 35 238645.0 36 371122.0 37 644617.0 38 655758.0 39 5740.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.29032704038737 21.12421743375969 10.536617368879218 30.048838156973723 2 12.70416477312701 22.879422563893893 36.52180692170348 27.894605741275612 3 15.053810637931203 27.73601720328595 28.790822995327524 28.419349163455323 4 10.48450874497627 18.680607776750342 37.80366349921383 33.03121997905955 5 11.970184988853966 38.221203915850865 35.09710707616421 14.711504019130963 6 29.789483536302207 39.79275935678725 17.664591980359475 12.753165126551064 7 25.57392672957231 33.18076608983363 23.748734454895125 17.496572725698925 8 24.029223710106784 37.34395088781165 21.039185226030956 17.58764017605061 9 24.412714809409234 17.023289128200883 22.185733541851505 36.37826252053838 10-11 23.080922486799004 27.915093234356547 28.88329512944002 20.120689149404427 12-13 23.63266097226639 25.481928350472145 29.595746530261646 21.289664146999822 14-15 21.36310921992351 26.633680864251296 26.644964880972523 25.358245034852672 16-17 20.769079886198202 30.402121277013084 27.49781807908181 21.330980757706904 18-19 20.629853703759018 29.32251571521267 29.54891842412774 20.498712156900567 20-21 22.035888537101638 28.523790262506544 28.54086581887459 20.89945538151723 22-23 20.912355212772045 28.719430808555725 29.012432979621916 21.355780999050317 24-25 21.227862659424478 28.190864593281805 28.781421583056904 21.79985116423681 26-27 21.223725709268663 28.527326341848696 28.712386586280786 21.536561362601855 28-29 20.795272268298234 28.35669220714144 29.33009106947027 21.517944455090056 30-31 21.708436959863022 28.645005828036403 28.389410715166104 21.257146496934478 32-33 20.54846517036413 28.830507992828743 29.21301461559191 21.40801222121522 34-35 20.833076229918273 28.1036669753107 29.281617378667725 21.781639416103303 36-37 21.50908651955484 28.72334301309989 28.552277035854235 21.215293431491038 38-39 21.047677627357153 28.642880774358687 28.933819382390514 21.375622215893646 40-41 21.48090152563635 28.40456285858157 28.515293513594326 21.599242102187755 42-43 20.996027182040987 28.668550980179447 28.92867403804932 21.40674779973025 44-45 21.511802478784105 27.96671230887886 28.75918128989533 21.762303922441703 46-47 21.44244328999531 28.705109414981685 28.60921185157144 21.243235443451564 48-49 21.17632769835629 28.50959505537512 28.803123678599384 21.510953567669205 50-51 21.668190388657965 28.37701746219589 28.217238072909424 21.73755407623672 52-53 20.937972964535184 28.801912848296684 28.474779513391862 21.78533467377627 54-55 21.458099980657032 28.368272133079625 28.206366985600734 21.96726090066261 56-57 21.55620414737324 28.496278533521984 28.19572207676685 21.751795242337923 58-59 21.058727008738614 28.036411788941884 28.88422101837142 22.020640183948085 60-61 21.81443764890114 28.429147615296845 27.996706858205272 21.75970787759674 62-63 20.867789449820663 28.850513187049458 28.559828640776264 21.721868722353616 64-65 21.058799249996806 27.980711221961467 28.672526373990774 22.287963154050953 66-67 21.385053273978688 28.459407975739946 28.312820642922286 21.842718107359072 68-69 21.391176168395916 28.318104763229456 28.454552799344015 21.836166269030613 70-71 21.683586988206756 28.178561274836166 28.014762245790315 22.123089491166763 72-73 21.136999537571004 28.608646522515972 28.575736692571084 21.678617247341933 74-75 21.613154439027586 27.8639708965072 28.3106968435349 22.212177820930318 76-77 21.395174854315062 28.5388288060708 28.32988237969702 21.73611395991712 78-79 21.337820502440596 28.32002835516645 28.59393638439243 21.74821475800053 80-81 21.65813418972954 28.21377520499085 28.085891502156734 22.04219910312288 82-83 20.91330087706058 28.625410703301803 28.52874711722848 21.932541302409142 84-85 21.27171708739513 27.884301231888337 28.294786747593147 22.54919493312339 86-87 21.37693544347886 28.440764419215835 28.138088261116117 22.044211876189188 88-89 21.072261080648243 28.009388149350446 28.652115850741666 22.26623491925965 90-91 21.522869871900507 28.093105108363925 28.156107807657182 22.227917212078385 92-93 20.787683265148694 28.573631046645776 28.541842930004442 22.096842758201092 94-95 20.927761740262042 27.753878911089558 28.660182941012348 22.658176407636052 96-97 21.18037510094215 28.247467968800173 28.36572926510157 22.20642766515611 98-99 20.949718208182173 27.996105698060997 28.517738520147272 22.536437573609554 100 21.233654001643018 27.84300350392945 28.226782640764934 22.696559853662595 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 8.0 1 7.5 2 10.0 3 14.0 4 15.5 5 25.5 6 51.5 7 111.5 8 185.0 9 199.0 10 173.5 11 168.0 12 196.5 13 244.0 14 300.5 15 391.0 16 477.5 17 582.0 18 728.0 19 925.0 20 1289.5 21 1772.0 22 2461.5 23 3764.0 24 5584.0 25 8215.5 26 12373.5 27 18229.0 28 26114.0 29 35252.5 30 44750.5 31 55001.5 32 65060.5 33 74603.0 34 83985.5 35 92527.0 36 99954.0 37 106802.0 38 112791.0 39 116738.0 40 119015.5 41 119511.0 42 117843.5 43 114787.5 44 109756.5 45 103619.5 46 97928.0 47 92772.0 48 88535.0 49 86337.0 50 91978.5 51 87562.5 52 71382.0 53 63213.0 54 58711.5 55 54190.5 56 49904.5 57 45125.5 58 40294.5 59 36078.5 60 31359.5 61 26659.5 62 22114.5 63 17815.5 64 14211.0 65 11484.5 66 9160.0 67 6940.5 68 5168.5 69 3847.5 70 2828.0 71 2124.0 72 1613.0 73 1146.0 74 843.5 75 596.0 76 381.5 77 246.5 78 143.0 79 76.5 80 39.5 81 18.5 82 9.5 83 5.0 84 4.0 85 2.5 86 1.5 87 1.0 88 0.5 89 1.0 90 1.0 91 0.5 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.8502343433224115 2 5.393620148308904 3 0.0 4 0.005720517120356442 5 0.16157762507874707 6 0.05537028835363877 7 0.03676961318870618 8 0.04234621792867631 9 0.04079915983952331 10-11 0.04225627269093485 12-13 0.04049334603120237 14-15 0.043281648401187416 16-17 0.034323102722138656 18-19 0.028494651316492467 20-21 0.02313391514710184 22-23 0.023385761812777905 24-25 0.020399579919761653 26-27 0.013455807566121442 28-29 0.019662028970281735 30-31 0.023745542763743722 32-33 0.001439123803863256 34-35 0.0038676452228825005 36-37 0.0047491085527487445 38-39 0.0067818709257055935 40-41 0.007285564257057733 42-43 0.00746545473254064 44-45 0.00876066615601757 46-47 0.016621879934620606 48-49 0.024968797997027493 50-51 0.02293603562407064 52-53 0.028260793698364685 54-55 0.024519071808320223 56-57 0.016441989459137697 58-59 0.015254712320950513 60-61 0.012952114234769302 62-63 0.018402795641901386 64-65 0.019698007065378314 66-67 0.02084930610846892 68-69 0.027181450845467248 70-71 0.027307374178305283 72-73 0.023889455144130045 74-75 0.02504075418722065 76-77 0.018960456115898396 78-79 0.015614493271916326 80-81 0.013725643279345804 82-83 0.008724688060920988 84-85 0.011548968526002628 86-87 0.009390282820207745 88-89 0.007933169968796198 90-91 0.00802311520653765 92-93 0.00462318521991071 94-95 0.0017449376121841977 96-97 0.0010973319004457327 98-99 8.454852347696628E-4 100 0.10332908911738177 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2779469.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.75800721079398 #Duplication Level Percentage of deduplicated Percentage of total 1 90.01651392235188 75.39603822198883 2 7.461760752823221 12.499644218803736 3 1.378528927862208 3.4638850764051274 4 0.43781925712914604 1.4668347398258992 5 0.21974661856091096 0.9202769430986185 6 0.12222495761583187 0.6142391328795499 7 0.08103216007670412 0.4750964573607556 8 0.05229783220978342 0.3504289765868746 9 0.040334529971929295 0.3040505867009556 >10 0.17988548984954686 2.5818567659739102 >50 0.006266069158182045 0.36971481958362407 >100 0.002684616200382475 0.4646122208813569 >500 3.8779979612755725E-4 0.19120681037171888 >1k 4.7397752797434354E-4 0.6845261547954232 >5k 4.3088866179485774E-5 0.21758887474358657 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 6029 0.21691193533728922 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 4556 0.16391620126002485 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.59780950965814E-5 0.0 5 0.0 3.59780950965814E-5 0.0 3.59780950965814E-5 0.0 6 0.0 3.59780950965814E-5 0.0 3.59780950965814E-5 0.0 7 0.0 3.59780950965814E-5 0.0 3.59780950965814E-5 0.0 8 0.0 3.59780950965814E-5 0.0 3.59780950965814E-5 0.0 9 0.0 3.59780950965814E-5 0.0 3.59780950965814E-5 0.0 10-11 0.0 3.59780950965814E-5 0.0 3.59780950965814E-5 0.0 12-13 0.0 3.59780950965814E-5 0.0 3.59780950965814E-5 0.0 14-15 0.0 3.59780950965814E-5 0.0 3.59780950965814E-5 0.0 16-17 0.0 3.59780950965814E-5 0.0 1.439123803863256E-4 0.0 18-19 3.59780950965814E-5 3.59780950965814E-5 0.0 1.439123803863256E-4 0.0 20-21 3.59780950965814E-5 3.59780950965814E-5 0.0 1.439123803863256E-4 0.0 22-23 3.59780950965814E-5 5.3967142644872104E-5 0.0 1.79890475482907E-4 0.0 24-25 3.59780950965814E-5 7.19561901931628E-5 0.0 2.158685705794884E-4 0.0 26-27 3.59780950965814E-5 1.079342852897442E-4 0.0 3.058138083209419E-4 0.0 28-29 3.59780950965814E-5 1.439123803863256E-4 0.0 4.8570428380384885E-4 0.0 30-31 3.59780950965814E-5 1.439123803863256E-4 0.0 0.00179890475482907 0.0 32-33 3.59780950965814E-5 1.439123803863256E-4 0.0 0.005684539025259861 0.0 34-35 3.59780950965814E-5 1.6190142793461628E-4 0.0 0.011477012335809465 0.0 36-37 3.59780950965814E-5 1.79890475482907E-4 0.0 0.020525503252599685 0.0 38-39 3.59780950965814E-5 1.79890475482907E-4 0.0 0.03741721890044465 0.0 40-41 3.59780950965814E-5 1.79890475482907E-4 0.0 0.05682740120505032 0.0 42-43 3.59780950965814E-5 1.79890475482907E-4 0.0 0.07780263064635727 0.0 44-45 3.59780950965814E-5 1.79890475482907E-4 0.0 0.10354495768796126 0.0 46-47 3.59780950965814E-5 2.518466656760698E-4 0.0 0.13560144041901528 0.0 48-49 3.59780950965814E-5 2.518466656760698E-4 0.0 0.16933090457206035 0.0 50-51 3.59780950965814E-5 2.518466656760698E-4 0.0 0.20779148823030585 0.0 52-53 3.59780950965814E-5 2.518466656760698E-4 0.0 0.24922026473401934 0.0 54-55 3.59780950965814E-5 2.518466656760698E-4 0.0 0.2942828288424876 0.0 56-57 3.59780950965814E-5 2.518466656760698E-4 0.0 0.34918540195987074 0.0 58-59 3.59780950965814E-5 2.518466656760698E-4 0.0 0.4122010355215331 0.0 60-61 3.59780950965814E-5 2.518466656760698E-4 0.0 0.4779869824056322 0.0 62-63 3.59780950965814E-5 2.518466656760698E-4 0.0 0.5492056216493151 0.0 64-65 3.59780950965814E-5 2.518466656760698E-4 0.0 0.6311817113268757 0.0 66-67 3.59780950965814E-5 2.518466656760698E-4 0.0 0.7235554704873485 0.0 68-69 3.59780950965814E-5 2.518466656760698E-4 0.0 0.8257872277042846 0.0 70-71 3.59780950965814E-5 2.518466656760698E-4 0.0 0.9483825867458857 0.0 72-73 3.59780950965814E-5 2.518466656760698E-4 0.0 1.078353455282286 0.0 74-75 3.59780950965814E-5 2.518466656760698E-4 0.0 1.2150702166492953 0.0 76-77 3.59780950965814E-5 2.518466656760698E-4 0.0 1.3657105008186816 0.0 78-79 3.59780950965814E-5 2.518466656760698E-4 0.0 1.537649817285244 0.0 80-81 3.59780950965814E-5 2.518466656760698E-4 0.0 1.7323093008052977 0.0 82-83 3.59780950965814E-5 2.698357132243605E-4 0.0 1.940316657606183 0.0 84-85 3.59780950965814E-5 2.878247607726512E-4 0.0 2.156077293900382 0.0 86-87 3.59780950965814E-5 2.878247607726512E-4 0.0 2.389683065362485 0.0 88 3.59780950965814E-5 2.878247607726512E-4 0.0 2.58779644601181 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGCGAC 120 0.005095153 19.563845 7 ACCGAAC 145 6.6475244E-4 19.428923 8 TAGCGCT 155 0.0010304903 18.20231 4 GTATCAA 10260 0.0 17.306345 1 GAGTACT 3515 0.0 16.763937 12-13 CATGGGG 4395 0.0 16.583635 4 GTATAGG 795 0.0 15.640743 1 GTACATG 13180 0.0 15.359019 1 CTATACT 1270 0.0 15.180534 4 ATCAACG 11380 0.0 15.123 3 TATCAAC 11495 0.0 15.087889 2 CAACGCA 11235 0.0 15.067332 5 AACGCAG 11325 0.0 15.01034 6 TCAACGC 11470 0.0 14.96361 4 TATGCGA 255 1.1248118E-4 14.752198 5 ACATGGG 12765 0.0 14.734596 3 TACATGG 13290 0.0 14.58316 2 TGTATCG 195 0.004773253 14.468502 5 CTAAGAC 1080 0.0 14.367767 3 TATACAG 1750 0.0 14.24114 5 >>END_MODULE