FastQCFastQC Report
Wed 25 May 2016
SRR1295132_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295132_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences859176
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT21060.2451185787312495No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA16780.19530340698529755No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTTAG750.003037448733.410291
GTATAGG2209.332871E-825.627211
TAAGACA2503.324203E-822.6922474
ATATACC1654.435331E-420.6276823
GTACATA2258.165177E-519.4893361
TAGAGTG1901.199763E-418.537185
CGAGACT1859.575849E-418.399124
GTAGCAC1900.001145907317.9135133
CTAGACC2552.2543198E-517.796433
ACTACGT1600.008993180517.7269133
CATGGGG20550.017.1158054
TAGTACT2000.001615070317.0191844
GTGTATG4501.8222636E-816.7051451
GTCTTAG2250.001829105716.7051451
GTAAGAC2050.001906153716.6027683
GTATCAG2300.002118559316.341991
GGACCGC2203.5860247E-416.0624086
GTGTTAT3155.8913938E-515.9096621
TGTAAGA3155.904626E-515.9054882
GTATTGG4051.865923E-615.4677281
Produced by FastQC (version 0.11.5)