##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295132_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 859176 Sequences flagged as poor quality 0 Sequence length 100 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.98628104136987 33.0 27.0 33.0 6.0 33.0 2 22.051091976498412 27.0 2.0 33.0 2.0 33.0 3 24.42207766511169 27.0 15.0 33.0 6.0 33.0 4 24.227987048055347 33.0 2.0 37.0 2.0 37.0 5 26.28648262986862 33.0 15.0 37.0 2.0 37.0 6 27.32695512910044 33.0 15.0 37.0 2.0 37.0 7 27.148935724461577 33.0 15.0 37.0 6.0 37.0 8 27.915225751184856 33.0 22.0 37.0 6.0 37.0 9 28.909391090998817 33.0 22.0 37.0 15.0 37.0 10-11 28.476167863161912 33.0 18.5 37.0 6.0 37.0 12-13 27.236101799864056 33.0 15.0 37.0 4.0 37.0 14-15 28.972805339069062 33.0 22.0 37.0 6.0 40.0 16-17 29.3953014283453 33.0 24.5 38.5 6.0 40.0 18-19 29.583685414862614 33.0 22.0 40.0 6.0 40.0 20-21 29.052137163980372 33.0 22.0 38.5 6.0 40.0 22-23 29.900639100719758 33.0 27.0 38.5 6.0 40.0 24-25 30.059336503812958 33.0 27.0 40.0 6.0 40.0 26-27 28.55980730374219 33.0 18.5 37.0 4.0 40.0 28-29 29.282360657187816 33.0 22.0 37.0 6.0 40.0 30-31 30.006986927009137 33.0 27.0 38.5 10.5 40.0 32-33 28.73135189995996 33.0 22.0 37.0 6.0 40.0 34-35 28.323237613713605 33.0 22.0 37.0 6.0 40.0 36-37 28.644337714275075 33.0 22.0 37.0 6.0 40.0 38-39 28.725107544903487 33.0 22.0 37.0 6.0 40.0 40-41 27.689877277763813 33.0 18.5 37.0 4.0 40.0 42-43 28.324218786372057 33.0 22.0 37.0 6.0 40.0 44-45 28.191933899457155 33.0 22.0 37.0 6.0 40.0 46-47 27.82900302149967 33.0 22.0 37.0 6.0 40.0 48-49 27.77870832053037 33.0 22.0 37.0 6.0 40.0 50-51 27.697595137666788 33.0 22.0 37.0 6.0 40.0 52-53 27.874351122470834 33.0 22.0 37.0 6.0 40.0 54-55 27.80832274178981 33.0 22.0 37.0 6.0 40.0 56-57 27.749557715764873 33.0 22.0 37.0 6.0 40.0 58-59 27.633969058726034 33.0 22.0 37.0 6.0 40.0 60-61 27.50657083065635 33.0 22.0 37.0 6.0 40.0 62-63 27.421143048688513 33.0 22.0 37.0 6.0 37.0 64-65 26.95112701006546 33.0 22.0 37.0 6.0 37.0 66-67 27.11199160591078 33.0 22.0 37.0 6.0 37.0 68-69 26.62534626199987 30.0 22.0 37.0 6.0 37.0 70-71 26.742804733838 27.0 22.0 35.0 6.0 37.0 72-73 26.748446767600583 27.0 22.0 33.0 10.5 37.0 74-75 26.8451772395877 27.0 22.0 33.0 10.5 37.0 76-77 26.59759234429267 27.0 22.0 33.0 10.5 37.0 78-79 26.17345980334646 27.0 22.0 33.0 6.0 37.0 80-81 25.98852970753373 27.0 22.0 33.0 6.0 37.0 82-83 26.121512937977784 27.0 22.0 33.0 10.5 37.0 84-85 26.072115026490497 27.0 22.0 33.0 6.0 37.0 86-87 25.85851443708856 27.0 22.0 33.0 10.5 37.0 88-89 25.823303956348873 27.0 22.0 33.0 6.0 37.0 90-91 25.981800003724498 27.0 22.0 33.0 6.0 37.0 92-93 25.88954649571217 27.0 22.0 33.0 6.0 37.0 94-95 25.76804054116968 27.0 22.0 33.0 6.0 37.0 96-97 25.874830651694182 27.0 22.0 33.0 6.0 37.0 98-99 25.932652332001823 27.0 22.0 33.0 10.5 37.0 100 25.605247353278024 27.0 22.0 33.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.359987230366677 1101 2 11.311321123771897 1101 3 8.568462107504896 1101 4 12.721679017235779 1101 5 10.787399016821091 1101 6 9.903898640368336 1101 7 9.587841797504389 1101 8 9.210218513940024 1101 9 7.32970059275269 1101 10-11 7.9467194462677035 1101 12-13 9.314063222986984 1101 14-15 8.03932581811921 1101 16-17 8.084727889348969 1101 18-19 8.339331894827872 1101 20-21 7.244939773813037 1101 22-23 7.560279048671497 1101 24-25 7.507714765143962 1101 26-27 8.585818109697737 1101 28-29 8.340226607290056 1101 30-31 5.330858627491704 1101 32-33 7.155364077269695 1101 34-35 8.898937075693748 1101 36-37 8.241380659382983 1101 38-39 8.278547599205233 1101 40-41 9.064799557358956 1101 42-43 8.632233512138125 1101 44-45 8.554492348603606 1101 46-47 7.408030003048523 1101 48-49 5.5522446409476025 1101 50-51 6.966403572297203 1101 52-53 5.865794860270796 1101 54-55 3.907431814833121 1101 56-57 6.1417959726845694 1101 58-59 6.168727063106271 1101 60-61 6.6979310249469926 1101 62-63 6.259210888191962 1101 64-65 6.496111442023036 1101 66-67 5.705711431155088 1101 68-69 6.83380513029617 1101 70-71 6.0538487780501455 1101 72-73 5.546340221482247 1101 74-75 4.966273447513849 1101 76-77 4.964343446274139 1101 78-79 5.103070151688129 1101 80-81 4.977095733861159 1101 82-83 4.5625355060082775 1101 84-85 4.398273269002278 1101 86-87 3.969329972917368 1101 88-89 3.846407368297392 1101 90-91 3.813572057528553 1101 92-93 3.8044931578112333 1101 94-95 3.565757722576926 1101 96-97 3.746522294665912 1101 98-99 3.412783995100998 1101 100 3.406218451567799 1102 1 6.918926146972478 1102 2 10.637332286440909 1102 3 8.28046076546526 1102 4 12.69109432157476 1102 5 10.687786791179171 1102 6 9.623471404231136 1102 7 6.720960804201265 1102 8 7.982066325676573 1102 9 6.395330630700439 1102 10-11 7.575841706320585 1102 12-13 9.263825459106013 1102 14-15 6.431768699676937 1102 16-17 7.476267801098963 1102 18-19 7.4975602450517655 1102 20-21 7.217163913811504 1102 22-23 6.754589097374939 1102 24-25 7.039193195006039 1102 26-27 8.454393306915787 1102 28-29 7.755520196628346 1102 30-31 4.182876047359947 1102 32-33 4.706820032812814 1102 34-35 4.9649821103287906 1102 36-37 5.28092465054587 1102 38-39 4.661385298406671 1102 40-41 6.2580360046360575 1102 42-43 7.024277748574502 1102 44-45 6.47941640466404 1102 46-47 5.432988548800811 1102 48-49 3.32288289101513 1102 50-51 5.114092429855681 1102 52-53 5.773102072280352 1102 54-55 5.210607107253626 1102 56-57 6.113153328285101 1102 58-59 5.891310634748539 1102 60-61 5.866105366554219 1102 62-63 4.172404209727944 1102 64-65 5.904841391672328 1102 66-67 3.5530186916137296 1102 68-69 5.893115136606664 1102 70-71 5.036915368893997 1102 72-73 4.8676041309115305 1102 74-75 4.007340405359514 1102 76-77 3.262134228593613 1102 78-79 4.403771361340642 1102 80-81 4.499300100577123 1102 82-83 3.949379187460398 1102 84-85 3.0034185314691335 1102 86-87 3.1742138828553657 1102 88-89 3.352223832265203 1102 90-91 3.2141796696064304 1102 92-93 3.2142361475315475 1102 94-95 2.9262011952134905 1102 96-97 3.203142347257394 1102 98-99 2.976720283343976 1102 100 3.0248908497611353 1103 1 6.61730622698472 1103 2 10.894572009822994 1103 3 8.024509035145869 1103 4 11.85716709045014 1103 5 10.321586862592646 1103 6 9.160702966073021 1103 7 8.283595159913254 1103 8 8.020210595473774 1103 9 7.1427145655830095 1103 10-11 7.389898866250338 1103 12-13 8.768698700184796 1103 14-15 8.47996642027536 1103 16-17 7.991566134541607 1103 18-19 7.022695238384074 1103 20-21 8.290083996301362 1103 22-23 6.827965533842452 1103 24-25 5.312895364372576 1103 26-27 5.821795605263809 1103 28-29 7.007014960854562 1103 30-31 6.122265680746477 1103 32-33 8.145486422570073 1103 34-35 7.1512829286689374 1103 36-37 4.290912517541912 1103 38-39 6.356342268143386 1103 40-41 6.847989658747068 1103 42-43 7.70533823870414 1103 44-45 5.6920254377401385 1103 46-47 6.547732881266981 1103 48-49 5.797745165024267 1103 50-51 6.458456594864536 1103 52-53 6.217009701134312 1103 54-55 4.728972394474258 1103 56-57 5.734886890433042 1103 58-59 5.484452390499872 1103 60-61 4.944075402042348 1103 62-63 2.7780642742915553 1103 64-65 5.047175555733858 1103 66-67 4.394976295327233 1103 68-69 5.202888398818004 1103 70-71 3.6081202527013545 1103 72-73 4.346811332013338 1103 74-75 3.381423142973283 1103 76-77 3.583666120540652 1103 78-79 2.9029907318818537 1103 80-81 4.127981902528603 1103 82-83 3.636960711773771 1103 84-85 3.5152518538718347 1103 86-87 2.825870966999389 1103 88-89 2.920014714658869 1103 90-91 2.774442077698012 1103 92-93 2.5617264739202845 1103 94-95 2.950912626470732 1103 96-97 3.2245600140770883 1103 98-99 2.988191073045865 1103 100 3.358719705177993 1104 1 6.432626747675862 1104 2 9.795020644898624 1104 3 7.705674186299895 1104 4 12.501110095619019 1104 5 9.601244084690485 1104 6 8.38875933014273 1104 7 4.022635680315496 1104 8 6.644834124800571 1104 9 6.7094771705795 1104 10-11 7.408024228877959 1104 12-13 7.65096539670429 1104 14-15 7.392139513726747 1104 16-17 7.485618138653585 1104 18-19 7.141158612090955 1104 20-21 7.795848120421724 1104 22-23 6.773236448277977 1104 24-25 6.226354958325931 1104 26-27 8.490178809676248 1104 28-29 7.3913505909298465 1104 30-31 6.524449210651067 1104 32-33 7.74928098213249 1104 34-35 8.109503110145251 1104 36-37 6.894564734475686 1104 38-39 7.48617559786549 1104 40-41 7.144051793561488 1104 42-43 7.224011003533651 1104 44-45 6.8385772552719395 1104 46-47 6.888219157391216 1104 48-49 5.450594364896187 1104 50-51 5.545234138313646 1104 52-53 5.9638905016716635 1104 54-55 4.789908882577663 1104 56-57 5.8477116302439605 1104 58-59 3.9600064734762945 1104 60-61 4.297666228074977 1104 62-63 3.785075777261188 1104 64-65 2.564413458716974 1104 66-67 3.2647926763456496 1104 68-69 4.088972465832793 1104 70-71 2.714838403462963 1104 72-73 2.549568024223433 1104 74-75 3.4298373919218115 1104 76-77 3.7462250136747386 1104 78-79 4.208669871735143 1104 80-81 2.96125203934605 1104 82-83 2.770121187589364 1104 84-85 3.0891719156960846 1104 86-87 0.5735080191768382 1104 88-89 0.3483634077549169 1104 90-91 2.2025599530431847 1104 92-93 2.389517773746224 1104 94-95 2.418648999496291 1104 96-97 2.8627590578235242 1104 98-99 2.42034419639824 1104 100 0.8944462002871845 1105 1 5.736198735128042 1105 2 -2.7634084462030657 1105 3 0.4848781990980946 1105 4 8.239903246801934 1105 5 8.528970141637167 1105 6 8.181332716344468 1105 7 5.277080501932797 1105 8 6.091328945093753 1105 9 5.149229223563463 1105 10-11 4.979475413481605 1105 12-13 5.067830079120736 1105 14-15 5.945493021795443 1105 16-17 6.085485787093177 1105 18-19 6.202391221011659 1105 20-21 7.076186059276388 1105 22-23 2.014447618533005 1105 24-25 4.116369848628818 1105 26-27 6.878255974427763 1105 28-29 6.075418869649354 1105 30-31 5.268574041224223 1105 32-33 7.115899017575508 1105 34-35 6.152097260916495 1105 36-37 6.1929212037525545 1105 38-39 3.9915214212378487 1105 40-41 5.298315391646092 1105 42-43 5.522384927086332 1105 44-45 6.129593298760771 1105 46-47 5.267059699942333 1105 48-49 4.815931918553041 1105 50-51 5.885166692584406 1105 52-53 5.503926096356896 1105 54-55 5.271046227264701 1105 56-57 5.152639333429786 1105 58-59 1.8175142797506894 1105 60-61 4.26329364308469 1105 62-63 -0.8134123815173488 1105 64-65 -0.5451154323226035 1105 66-67 0.8371587057506851 1105 68-69 2.571780906961326 1105 70-71 2.6333645523756246 1105 72-73 2.6095692038917306 1105 74-75 2.484300450247982 1105 76-77 1.5931666634288142 1105 78-79 2.842730145562637 1105 80-81 1.369024247805477 1105 82-83 0.6825633176369621 1105 84-85 1.0814842100064723 1105 86-87 -0.6700573717628266 1105 88-89 1.4705033140074022 1105 90-91 1.1780794284037697 1105 92-93 1.6127579614338536 1105 94-95 0.9340830933545519 1105 96-97 1.9335719576167207 1105 98-99 1.8486688146310932 1105 100 1.9504678050694153 1106 1 6.169932375199053 1106 2 10.12754783429775 1106 3 6.71962925614708 1106 4 11.676015393660805 1106 5 9.696700306285884 1106 6 9.151720687912604 1106 7 6.76193591545411 1106 8 7.4440168864102425 1106 9 7.097750410272436 1106 10-11 5.7645723125700705 1106 12-13 8.094873941670361 1106 14-15 8.025470532389384 1106 16-17 7.9095680364089915 1106 18-19 7.196375480772502 1106 20-21 8.276713176716115 1106 22-23 6.123681583398877 1106 24-25 5.005747884053729 1106 26-27 7.696147188081806 1106 28-29 6.300688730796615 1106 30-31 5.37174858856336 1106 32-33 7.4389464962527825 1106 34-35 7.061290714365295 1106 36-37 5.9280773749722115 1106 38-39 7.1042507416544325 1106 40-41 6.4567346725010175 1106 42-43 6.1297179039660925 1106 44-45 4.337788271827417 1106 46-47 5.806878436267532 1106 48-49 5.676320253610012 1106 50-51 6.456219086568783 1106 52-53 5.364748566064208 1106 54-55 5.350856357112296 1106 56-57 4.977663429221995 1106 58-59 5.051790749359505 1106 60-61 4.841074684445029 1106 62-63 4.786895587330488 1106 64-65 5.207202102144343 1106 66-67 3.1597471332041778 1106 68-69 3.1311315986454034 1106 70-71 3.102462138575241 1106 72-73 2.6878262983730323 1106 74-75 2.6878146251400494 1106 76-77 3.561107827484225 1106 78-79 4.141162101625042 1106 80-81 3.697434652497531 1106 82-83 2.061676311073054 1106 84-85 2.7886757780035047 1106 86-87 3.300189308177046 1106 88-89 3.303165072402237 1106 90-91 3.005298527748785 1106 92-93 2.9139295680310617 1106 94-95 1.3595183419222892 1106 96-97 1.4751694441500476 1106 98-99 1.7063046011623122 1106 100 2.7326183926315295 1107 1 5.946050082899362 1107 2 10.304374894753309 1107 3 5.9605604034385316 1107 4 11.269271670478467 1107 5 9.051859848674653 1107 6 7.782716284079111 1107 7 3.690989685119437 1107 8 6.246508874984951 1107 9 5.722453909699901 1107 10-11 4.542368268284964 1107 12-13 5.989033615516849 1107 14-15 6.040109617535933 1107 16-17 6.8692129679644935 1107 18-19 6.060673352425951 1107 20-21 5.3307329288145375 1107 22-23 5.664172145542054 1107 24-25 4.598135559777528 1107 26-27 7.105899880370426 1107 28-29 6.272279161597389 1107 30-31 5.010136086703927 1107 32-33 4.929956777629954 1107 34-35 6.464142885550924 1107 36-37 6.340507220947586 1107 38-39 6.229402667567626 1107 40-41 7.551821411530934 1107 42-43 6.084595331177152 1107 44-45 3.982977697783859 1107 46-47 3.2366311516189086 1107 48-49 3.958970631435637 1107 50-51 4.814465146584993 1107 52-53 3.861963449845206 1107 54-55 5.20313694668862 1107 56-57 4.9897607102382295 1107 58-59 2.736456120283595 1107 60-61 2.6453045959186348 1107 62-63 0.18281725582872 1107 64-65 2.151096756081909 1107 66-67 4.101250899928999 1107 68-69 4.802343742570848 1107 70-71 3.969901157762301 1107 72-73 2.246803390136286 1107 74-75 2.3474427785396728 1107 76-77 2.90239987809775 1107 78-79 3.600635936350404 1107 80-81 1.934304448242223 1107 82-83 1.3300878867321622 1107 84-85 2.0471960253746424 1107 86-87 2.0217099741372238 1107 88-89 2.2393381710797016 1107 90-91 1.3704874866843895 1107 92-93 2.1689439880200148 1107 94-95 2.1969548760972586 1107 96-97 2.259690211586353 1107 98-99 1.9045196302386813 1107 100 0.06268507859403627 1108 1 4.024004658020832 1108 2 -3.261033524104384 1108 3 0.02331331399328107 1108 4 6.294966234361649 1108 5 6.851463371200815 1108 6 6.509567002494951 1108 7 4.031169822701656 1108 8 4.860237460747502 1108 9 4.608637367227736 1108 10-11 3.0627369693660462 1108 12-13 2.2295013771418404 1108 14-15 3.7372860110755397 1108 16-17 4.330754456920328 1108 18-19 3.638171196926333 1108 20-21 4.208561619396839 1108 22-23 3.9894887196674276 1108 24-25 2.827345702922873 1108 26-27 4.207007798771254 1108 28-29 5.918114887595998 1108 30-31 4.3547793270067 1108 32-33 4.397754817820008 1108 34-35 3.0067080505481876 1108 36-37 1.1633614273791828 1108 38-39 2.339400796386677 1108 40-41 5.139297580584845 1108 42-43 2.6193924340960244 1108 44-45 3.2678899287525773 1108 46-47 4.52391359181815 1108 48-49 4.608959650852778 1108 50-51 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50-51 -23.30592994150897 2213 52-53 -17.69318108527797 2213 54-55 -17.44575861618001 2213 56-57 -17.286318833586236 2213 58-59 -17.704116576219587 2213 60-61 -19.062522503533536 2213 62-63 -17.282706826127814 2213 64-65 -17.347100709594017 2213 66-67 -8.267039890282177 2213 68-69 -22.336470993147252 2213 70-71 -16.999657634175836 2213 72-73 -9.04695477497334 2213 74-75 -3.7950578459796915 2213 76-77 -3.1295163852262036 2213 78-79 -4.430790857734539 2213 80-81 -2.8783072042745026 2213 82-83 -2.588344636137144 2213 84-85 -2.5220633471390883 2213 86-87 -3.2046186427045775 2213 88-89 -2.738428939526351 2213 90-91 -2.5797245000289166 2213 92-93 -3.1883076789393776 2213 94-95 -3.1376286622020473 2213 96-97 -3.1338710462411328 2213 98-99 -3.2447719863673044 2213 100 -2.9329891189248194 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 266.0 3 263.0 4 1520.0 5 5363.0 6 7376.0 7 8395.0 8 7594.0 9 7451.0 10 8532.0 11 10624.0 12 13304.0 13 17096.0 14 13837.0 15 15495.0 16 17520.0 17 18296.0 18 18067.0 19 18525.0 20 18181.0 21 18163.0 22 19384.0 23 21252.0 24 23247.0 25 25152.0 26 26916.0 27 29073.0 28 30620.0 29 31838.0 30 33846.0 31 37667.0 32 43776.0 33 52050.0 34 61470.0 35 69032.0 36 68129.0 37 48893.0 38 10957.0 39 6.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.40035873022748 19.546720917819442 13.276457946651618 24.776462405301462 2 17.3778454866804 21.092334267904462 35.8804711242624 25.64934912115274 3 20.08677358337443 25.168459363119354 27.898268898225197 26.84649815528102 4 14.582125974632154 18.57604245308139 33.489128289628255 33.352703282658204 5 15.292871461038743 36.914474963294694 32.7695577262153 15.023095849451265 6 32.61723147481757 34.175328909266725 17.704031765822066 15.50340785009364 7 27.999587659304776 31.313203301779936 21.17216751213478 19.51504152678051 8 26.494521565472628 33.01968021260901 20.133985827779505 20.35181239413886 9 26.209291335855294 17.28746632094868 19.95878104719892 36.54446129599711 10-11 24.895033873505852 26.923615291378688 26.189966562741468 21.991384272373995 12-13 25.653447653347104 24.980755507124215 25.75752134849214 23.60827549103654 14-15 24.048046210874556 26.17169279313183 23.518959112996935 26.261301882996673 16-17 23.24738944399765 28.578835825635966 25.153750635379325 23.020024094987058 18-19 23.712518463347905 27.796899411429848 26.165667326206293 22.324914799015957 20-21 24.40009367006061 27.111931255874318 25.71849339236844 22.769481681696625 22-23 24.007589614277418 26.84642831634158 25.20938205509613 23.93660001428487 24-25 24.035269151669837 27.218307617273823 24.756340825514254 23.990082405542086 26-27 24.022997839892156 26.464991635672636 25.285020545386626 24.22698997904858 28-29 24.061662365978556 26.268327932469195 26.033477856457033 23.636531845095217 30-31 24.596967834410083 26.728856162148883 25.20794663466767 23.466229368773362 32-33 23.56266363674923 26.56959534407257 25.582794250526014 24.28494676865218 34-35 23.52217394738921 26.404297337862392 25.571877715459358 24.501650999289044 36-37 24.50828346456693 26.366425196850397 25.016944881889764 24.108346456692914 38-39 24.27523474178404 26.194186394965534 25.26077564678853 24.26980321646189 40-41 24.045362029081723 26.62442843946283 25.051398634687583 24.27881089676787 42-43 24.46779148879444 26.46225520598965 25.161172420224272 23.908780884991643 44-45 24.669961787516588 26.07531603592836 25.23674174349691 24.017980433058142 46-47 24.005863708596582 26.384697612081826 25.85967667470977 23.74976200461182 48-49 24.263334655977754 26.13203070074431 25.599428325939282 24.005206317338654 50-51 23.926852141294155 26.27677399187246 25.51103469834323 24.285339168490154 52-53 23.514039105895304 26.4197384073875 25.115146225747274 24.951076260969916 54-55 23.87551448557595 26.711045213207747 24.89980989411902 24.51363040709729 56-57 24.193926754557268 26.067313195626596 24.909296801499398 24.829463248316742 58-59 24.06306415749358 25.60249829411768 25.61676182740037 24.717675720988368 60-61 24.428947698788118 26.096794599120738 25.263785271415145 24.210472430676 62-63 23.607758592184535 26.12798442654341 25.695842649411453 24.5684143318606 64-65 23.413039959194766 26.212229963134792 25.57559505083855 24.799135026831884 66-67 24.02211232953284 25.977618358236736 25.13580817308757 24.864461139142858 68-69 24.48612245526977 25.791243508144678 25.011635254049224 24.71099878253633 70-71 24.02309167630217 26.133102280844938 25.155101168296067 24.688704874556823 72-73 24.470218785643397 25.887985305662948 25.076952702602618 24.564843206091037 74-75 24.547608548666844 25.64500102889732 25.06934767910163 24.738042743334216 76-77 24.078255878501732 25.922038768469946 25.637881228984305 24.36182412404402 78-79 24.254738609098485 25.87222734646788 25.650622669992316 24.222411374441315 80-81 23.88440344757044 25.82188745594662 25.674988175890267 24.61872092059268 82-83 23.79045080832549 25.904446951123095 25.321575586648038 24.983526653903372 84-85 23.95550642792528 25.90077163446209 25.009683811737254 25.134038125875378 86-87 24.233285850924737 25.400124604760137 25.434698038206278 24.93189150610885 88-89 24.22925580628564 25.25836189027914 25.771242876897414 24.741139426537806 90-91 24.340343051971555 25.40984085097439 25.503310253027102 24.74650584402695 92-93 23.571432744950247 25.59571596347 25.731797811238295 25.101053480341463 94-95 23.526960887726254 25.49072003525402 25.85257771589572 25.129741361124005 96-97 24.21451844770076 25.404881660791006 25.52479669510182 24.855803196406416 98-99 24.115750922610722 25.202986063398836 25.485358200028323 25.195904813962116 100 23.839575284378522 25.14164794998168 25.923008589583183 25.09576817605661 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 856.0 1 946.0 2 863.5 3 608.0 4 475.0 5 391.5 6 374.5 7 434.5 8 535.0 9 670.0 10 830.0 11 972.5 12 1218.0 13 1538.0 14 1809.0 15 2106.0 16 2357.5 17 2591.0 18 2882.5 19 3041.5 20 3185.0 21 3424.5 22 3628.5 23 3903.0 24 4319.0 25 4728.0 26 5234.0 27 5981.5 28 6878.5 29 7762.5 30 8521.5 31 9395.0 32 10453.5 33 11362.0 34 12479.0 35 13884.0 36 15349.0 37 17084.0 38 18681.0 39 20066.0 40 21740.0 41 23104.0 42 24188.5 43 25627.5 44 26686.5 45 27663.5 46 28988.5 47 30380.5 48 31855.0 49 34147.0 50 37537.5 51 37456.5 52 32923.0 53 29164.0 54 27122.0 55 25318.0 56 23503.0 57 21671.5 58 20122.0 59 18724.0 60 17026.0 61 15129.5 62 13099.5 63 11019.0 64 9236.5 65 7641.0 66 6126.0 67 4900.0 68 3864.5 69 2977.5 70 2269.5 71 1736.5 72 1326.0 73 1038.5 74 803.5 75 556.0 76 371.5 77 248.0 78 171.5 79 102.5 80 64.5 81 53.0 82 33.5 83 22.5 84 15.5 85 7.5 86 5.5 87 7.0 88 4.0 89 1.5 90 1.0 91 0.5 92 0.0 93 0.5 94 0.5 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content fail #Base N-Count 1 8.634435784984683 2 25.816247194986826 3 6.778704246859782 4 25.690894531504604 5 13.19531737385588 6 10.568614579550639 7 8.545164203841821 8 9.431594923508104 9 5.91380578600892 10-11 7.287156531374247 12-13 10.867680195908639 14-15 8.299754648640093 16-17 8.75204847435217 18-19 7.1754797620045245 20-21 8.79616050727674 22-23 6.300047952922334 24-25 5.855668687207278 26-27 10.421438680782517 28-29 6.902427442107322 30-31 4.7507146382114955 32-33 9.445910965855656 34-35 7.913221505256199 36-37 7.614970623015541 38-39 6.785105729210312 40-41 9.53448420346937 42-43 7.014220602065234 44-45 6.902718418577799 46-47 6.470036406975986 48-49 4.228411873702245 50-51 6.916627093866682 52-53 5.0473360522174735 54-55 5.408787023846104 56-57 5.235481438028995 58-59 3.7118704433084724 60-61 4.506294403009395 62-63 3.2012649329124647 64-65 4.731684777042189 66-67 2.760144603666769 68-69 5.59413903554103 70-71 2.8115892436474015 72-73 1.7823472722701752 74-75 1.0185340372636107 76-77 1.245728465413373 78-79 1.8894266134063336 80-81 2.4263363967336145 82-83 0.9962452396249429 84-85 1.4434760747506914 86-87 0.52067329627457 88-89 0.8067613620492192 90-91 0.31960855517379444 92-93 0.4005000139668706 94-95 0.6913600938573703 96-97 0.6581887762227995 98-99 0.5594895574364275 100 0.5550667150851513 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 859176.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.21137811858196 #Duplication Level Percentage of deduplicated Percentage of total 1 94.70827530792315 82.59639208838045 2 3.291441524137559 5.741023026335247 3 0.8562312902905875 2.240193324434812 4 0.4009653162747715 1.398749512403036 5 0.2040139840962434 0.8896170354247924 6 0.12385717445840186 0.6481052924634513 7 0.08836260691916975 0.5394357306499938 8 0.06343253149164223 0.4425630791149175 9 0.04448146779320446 0.3491361096284411 >10 0.20877680673164162 3.108912411539844 >50 0.004680908840298255 0.2621857841535524 >100 0.00427791695115999 0.8055095697750234 >500 9.357942939448178E-4 0.5370063678686486 >1k 2.673697982699479E-4 0.44117066782782605 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 2106 0.2451185787312495 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1678 0.19530340698529755 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.1639058819147648E-4 0.0 10-11 0.0 0.0 0.0 1.1639058819147648E-4 0.0 12-13 0.0 0.0 0.0 1.1639058819147648E-4 0.0 14-15 0.0 0.0 0.0 1.1639058819147648E-4 0.0 16-17 0.0 0.0 0.0 1.1639058819147648E-4 0.0 18-19 0.0 0.0 0.0 1.1639058819147648E-4 0.0 20-21 0.0 0.0 0.0 1.7458588228721472E-4 0.0 22-23 0.0 0.0 0.0 4.073670586701677E-4 0.0 24-25 0.0 0.0 0.0 8.729294114360736E-4 0.0 26-27 0.0 5.819529409573824E-5 0.0 0.0018040541169678853 0.0 28-29 0.0 1.1639058819147648E-4 0.0 0.003258936469361341 0.0 30-31 0.0 1.1639058819147648E-4 0.0 0.009136661173030905 0.0 32-33 0.0 1.1639058819147648E-4 0.0 0.022987141167816607 0.0 34-35 0.0 1.1639058819147648E-4 0.0 0.05057171056919653 0.0 36-37 0.0 1.1639058819147648E-4 0.0 0.08822406584913917 0.0 38-39 0.0 1.1639058819147648E-4 0.0 0.1417055411231226 0.0 40-41 0.0 1.1639058819147648E-4 0.0 0.2074080281572111 0.0 42-43 0.0 1.1639058819147648E-4 0.0 0.26956060225145956 0.0 44-45 0.0 1.1639058819147648E-4 0.0 0.35266348222017374 0.0 46-47 0.0 1.1639058819147648E-4 0.0 0.4414695010102703 0.0 48-49 0.0 1.1639058819147648E-4 0.0 0.5268419974487183 0.0 50-51 0.0 1.1639058819147648E-4 0.0 0.6173356797675913 5.819529409573824E-5 52-53 0.0 1.1639058819147648E-4 0.0 0.7145800162015699 1.1639058819147648E-4 54-55 0.0 1.1639058819147648E-4 0.0 0.8253256608657598 1.1639058819147648E-4 56-57 0.0 2.3278117638295297E-4 0.0 0.9655763196364889 1.1639058819147648E-4 58-59 0.0 2.3278117638295297E-4 0.0 1.1112391407581217 1.1639058819147648E-4 60-61 0.0 2.3278117638295297E-4 0.0 1.2640017877594345 1.1639058819147648E-4 62-63 0.0 2.3278117638295297E-4 0.0 1.4225839641703213 1.1639058819147648E-4 64-65 0.0 2.3278117638295297E-4 0.0 1.6135227240984384 1.1639058819147648E-4 66-67 0.0 2.3278117638295297E-4 0.0 1.8282633593117126 1.1639058819147648E-4 68-69 0.0 2.3278117638295297E-4 0.0 2.076524483924132 1.1639058819147648E-4 70-71 0.0 2.3278117638295297E-4 0.0 2.3711672579308547 1.1639058819147648E-4 72-73 0.0 2.909764704786912E-4 0.0 2.6670321331135876 1.1639058819147648E-4 74-75 0.0 3.4917176457442944E-4 0.0 2.9758745588796707 1.1639058819147648E-4 76-77 0.0 3.4917176457442944E-4 0.0 3.3232422693371326 1.1639058819147648E-4 78-79 0.0 3.4917176457442944E-4 0.0 3.7101245844856 1.1639058819147648E-4 80-81 0.0 3.4917176457442944E-4 0.0 4.140246003147201 1.1639058819147648E-4 82-83 0.0 3.4917176457442944E-4 0.0 4.614130282968798 1.1639058819147648E-4 84-85 0.0 3.4917176457442944E-4 0.0 5.09243740514167 1.1639058819147648E-4 86-87 0.0 3.4917176457442944E-4 0.0 5.5912292708362425 1.1639058819147648E-4 88 0.0 3.4917176457442944E-4 0.0 6.019954002439547 1.1639058819147648E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGTTAG 75 0.0030374487 33.41029 1 GTATAGG 220 9.332871E-8 25.62721 1 TAAGACA 250 3.324203E-8 22.692247 4 ATATACC 165 4.435331E-4 20.627682 3 GTACATA 225 8.165177E-5 19.489336 1 TAGAGTG 190 1.199763E-4 18.53718 5 CGAGACT 185 9.575849E-4 18.39912 4 GTAGCAC 190 0.0011459073 17.913513 3 CTAGACC 255 2.2543198E-5 17.79643 3 ACTACGT 160 0.0089931805 17.726913 3 CATGGGG 2055 0.0 17.115805 4 TAGTACT 200 0.0016150703 17.019184 4 GTGTATG 450 1.8222636E-8 16.705145 1 GTCTTAG 225 0.0018291057 16.705145 1 GTAAGAC 205 0.0019061537 16.602768 3 GTATCAG 230 0.0021185593 16.34199 1 GGACCGC 220 3.5860247E-4 16.062408 6 GTGTTAT 315 5.8913938E-5 15.909662 1 TGTAAGA 315 5.904626E-5 15.905488 2 GTATTGG 405 1.865923E-6 15.467728 1 >>END_MODULE