FastQCFastQC Report
Wed 25 May 2016
SRR1295132_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295132_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences859176
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT60180.7004385597363055No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA41420.4820898162890956No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23820.277242381072097No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19140.22277158579848597No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC18640.21695205638891216No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG18320.2132275575667849No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC18120.2108997458029554No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC16000.18622494110636237No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG15980.18599215992997942No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG12270.14281125171094167No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG11250.13093941171541104No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT10020.11662336936785944No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT9800.11406277642764695No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9680.11266608936934923No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAAGG1705.361208E-520.5868572
TTAGACC1150.00395493120.4373684
TCTAGGG3503.6379788E-1220.1454053
CGATTCG1200.00509704419.5607229
GTACTTT28350.019.373314-15
GAGTACT28400.019.173912-13
CCTAGGT1250.006405192418.802383
TATACTG1250.006405192418.802385
CAGAACG1508.2810316E-418.8023784
GGTCTAG2401.8737355E-618.768531
CATTACG1300.00803144418.079214
ATTACGA1350.00998155117.4096095
AGTACTT29100.016.85753612-13
CATGGGG26950.016.5698134
CACACCG2002.9883196E-416.4520825
TACTTTT30350.016.39524814-15
ACTTTTT35000.016.22744816-17
TAATAGA2901.1471275E-616.2089484
ATTAGAC2053.6068982E-416.0508123
GCATATA2203.8295618E-415.9248141
Produced by FastQC (version 0.11.5)