##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295132_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 859176 Sequences flagged as poor quality 0 Sequence length 100 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.158564717822657 33.0 33.0 33.0 27.0 33.0 2 29.677485171839063 33.0 33.0 33.0 22.0 33.0 3 30.61532445040364 33.0 33.0 33.0 27.0 33.0 4 34.93069173254374 37.0 37.0 37.0 33.0 37.0 5 35.27072799985102 37.0 37.0 37.0 33.0 37.0 6 35.02087232418038 37.0 37.0 37.0 33.0 37.0 7 34.90464701062413 37.0 37.0 37.0 33.0 37.0 8 34.91002076408093 37.0 37.0 37.0 33.0 37.0 9 34.84489324655251 37.0 37.0 37.0 33.0 37.0 10-11 34.80957976014227 37.0 37.0 37.0 30.0 37.0 12-13 34.69584462322039 37.0 37.0 37.0 27.0 37.0 14-15 36.033475097069754 37.0 37.0 40.0 27.0 40.0 16-17 36.008334730020394 37.0 37.0 40.0 27.0 40.0 18-19 36.03589311153943 37.0 37.0 40.0 27.0 40.0 20-21 36.039935356667314 37.0 37.0 40.0 27.0 40.0 22-23 36.01150870136037 37.0 37.0 40.0 27.0 40.0 24-25 35.96815611702375 37.0 37.0 40.0 27.0 40.0 26-27 35.90656861923517 37.0 37.0 40.0 27.0 40.0 28-29 35.82696211253573 37.0 37.0 40.0 27.0 40.0 30-31 35.67947079527361 37.0 37.0 40.0 27.0 40.0 32-33 35.60008252092703 37.0 37.0 40.0 27.0 40.0 34-35 35.46035969347375 37.0 37.0 40.0 27.0 40.0 36-37 35.42544135311042 37.0 37.0 40.0 27.0 40.0 38-39 35.3193472583033 37.0 33.0 40.0 27.0 40.0 40-41 35.19512998500889 37.0 33.0 40.0 27.0 40.0 42-43 35.02486219354358 37.0 33.0 40.0 27.0 40.0 44-45 34.84026497481308 37.0 33.0 40.0 22.0 40.0 46-47 34.61112856969934 37.0 33.0 40.0 22.0 40.0 48-49 34.408356378669794 37.0 33.0 40.0 22.0 40.0 50-51 34.25055343724685 37.0 33.0 40.0 22.0 40.0 52-53 34.12620813430543 37.0 33.0 40.0 22.0 40.0 54-55 33.958715094462605 37.0 33.0 40.0 22.0 40.0 56-57 33.77978609737703 37.0 33.0 40.0 22.0 40.0 58-59 33.60199074462042 37.0 33.0 40.0 22.0 40.0 60-61 33.39072378651173 37.0 33.0 37.0 22.0 40.0 62-63 33.14951360373195 37.0 33.0 37.0 22.0 40.0 64-65 32.965151493989595 37.0 33.0 37.0 22.0 40.0 66-67 32.704092642252576 37.0 33.0 37.0 22.0 40.0 68-69 32.47230776930454 37.0 33.0 37.0 22.0 40.0 70-71 32.22944193040774 37.0 33.0 37.0 22.0 37.0 72-73 31.99603166289561 37.0 33.0 37.0 22.0 37.0 74-75 31.804356150544244 33.0 33.0 37.0 22.0 37.0 76-77 31.5974136847398 33.0 33.0 37.0 22.0 37.0 78-79 31.403640813989217 33.0 27.0 37.0 18.5 37.0 80-81 31.25484359432759 33.0 27.0 37.0 15.0 37.0 82-83 31.104323793960724 33.0 27.0 37.0 15.0 37.0 84-85 30.96082234606181 33.0 27.0 37.0 15.0 37.0 86-87 31.032124966246727 33.0 27.0 37.0 15.0 37.0 88-89 31.09159008165964 33.0 27.0 37.0 15.0 37.0 90-91 31.150777605519707 33.0 27.0 37.0 15.0 37.0 92-93 31.17550536793393 33.0 27.0 37.0 15.0 37.0 94-95 31.25933045150237 33.0 27.0 37.0 15.0 37.0 96-97 31.237383842192983 33.0 27.0 37.0 15.0 37.0 98-99 31.17457016955781 33.0 27.0 37.0 15.0 37.0 100 30.594452126223263 33.0 27.0 37.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6000158222797829 1101 2 4.129239681404449 1101 3 2.249825009582956 1101 4 1.1920214497304968 1101 5 0.5374012579581446 1101 6 0.5153679598546574 1101 7 0.5368683181121199 1101 8 0.5159945197787863 1101 9 0.6127575042427509 1101 10-11 0.5798570517597526 1101 12-13 0.6589662679096122 1101 14-15 0.9605222075601318 1101 16-17 0.8516350487025477 1101 18-19 0.7918478247022165 1101 20-21 0.7294224455704708 1101 22-23 0.9230541184541181 1101 24-25 0.8697452879010399 1101 26-27 0.972116325312868 1101 28-29 0.8265249154778971 1101 30-31 0.806759677173396 1101 32-33 0.9415676868537517 1101 34-35 1.115986830321937 1101 36-37 1.0435947937705095 1101 38-39 1.1331050234782225 1101 40-41 1.0144915603679436 1101 42-43 1.011845246806466 1101 44-45 0.9446691428907528 1101 46-47 0.89664473061341 1101 48-49 0.8833482697337658 1101 50-51 0.9095767882300407 1101 52-53 0.8757765359813519 1101 54-55 0.989087858238122 1101 56-57 0.8952150236408087 1101 58-59 0.8420253136402067 1101 60-61 0.811928182284511 1101 62-63 0.7962171462611565 1101 64-65 0.9251025942294788 1101 66-67 0.7136888503457683 1101 68-69 0.7021866371829191 1101 70-71 0.7702849236491929 1101 72-73 0.8280000818611235 1101 74-75 0.8055955102642436 1101 76-77 0.710772051107778 1101 78-79 0.6538832611703604 1101 80-81 0.719768185698836 1101 82-83 0.7773091005127135 1101 84-85 0.8119761555597726 1101 86-87 0.7829305243467282 1101 88-89 0.7453299503088182 1101 90-91 0.7192613649017012 1101 92-93 0.8472425542237971 1101 94-95 0.9104084676329549 1101 96-97 0.6821780195185987 1101 98-99 0.7739389266657142 1101 100 0.9003229670694957 1102 1 0.5358235109072069 1102 2 4.09558573625608 1102 3 2.4155714464556155 1102 4 1.175670489409086 1102 5 0.6287420136911805 1102 6 0.8032172928924552 1102 7 0.6534625533978371 1102 8 0.5727274890172538 1102 9 0.6665992743784912 1102 10-11 0.6759795807050537 1102 12-13 0.6903552183692057 1102 14-15 0.9930444123810531 1102 16-17 0.8347531166984155 1102 18-19 1.0460610781914639 1102 20-21 0.9815881196365197 1102 22-23 1.0076693842760704 1102 24-25 1.0376489202500068 1102 26-27 1.0170477032411611 1102 28-29 0.922216550380135 1102 30-31 1.0174449107403802 1102 32-33 1.1542900530101292 1102 34-35 1.2392249490924527 1102 36-37 1.2165948155725985 1102 38-39 1.1153239067510583 1102 40-41 1.2095077544743518 1102 42-43 1.0830865971308938 1102 44-45 1.2656376643094873 1102 46-47 1.1961054923056835 1102 48-49 1.1931718363373278 1102 50-51 1.269845541356517 1102 52-53 1.2135147994934954 1102 54-55 1.21623472800335 1102 56-57 1.242334392494591 1102 58-59 1.203988422084997 1102 60-61 1.374394040820441 1102 62-63 1.2472781206449213 1102 64-65 1.235660921476395 1102 66-67 1.2505738952035657 1102 68-69 1.201325606113734 1102 70-71 1.131724781984076 1102 72-73 1.0983125118370545 1102 74-75 0.9779021045479759 1102 76-77 1.118967849603191 1102 78-79 0.9790006896383154 1102 80-81 0.9466664511004979 1102 82-83 1.091627833714508 1102 84-85 1.0722755224513634 1102 86-87 0.9762148150945258 1102 88-89 0.9860112231631604 1102 90-91 0.9710851701170036 1102 92-93 1.1110679546251951 1102 94-95 1.092027127313262 1102 96-97 1.093370545278372 1102 98-99 1.0830263699954266 1102 100 1.1485470053248932 1103 1 0.5511938043582987 1103 2 4.094882532122842 1103 3 2.2977811105936645 1103 4 1.1202849659410106 1103 5 0.39669740212949023 1103 6 0.4471883194247468 1103 7 0.4115840146050118 1103 8 0.39722340460360783 1103 9 0.5890300288108676 1103 10-11 0.5901750131573777 1103 12-13 0.6373070288465854 1103 14-15 0.8829282926104511 1103 16-17 0.8840019999519555 1103 18-19 0.9490844916099022 1103 20-21 0.7712189556842972 1103 22-23 0.808752792302073 1103 24-25 0.7936862738705983 1103 26-27 0.7129993606896647 1103 28-29 0.831185317914688 1103 30-31 0.8134309996865952 1103 32-33 0.7527368098909619 1103 34-35 0.9465390007870624 1103 36-37 0.9094795192430993 1103 38-39 0.8852723141576604 1103 40-41 0.7885207546419579 1103 42-43 0.8032004214683113 1103 44-45 0.9022268692151627 1103 46-47 0.986204523851498 1103 48-49 0.7973908789595185 1103 50-51 0.8944247119399975 1103 52-53 0.9128804583556942 1103 54-55 0.8531099511704596 1103 56-57 0.9088125057213858 1103 58-59 0.916598366047289 1103 60-61 0.8307999389906584 1103 62-63 0.9901477036047908 1103 64-65 0.9878925926246254 1103 66-67 0.931573355958939 1103 68-69 1.064719094370588 1103 70-71 1.0180698693927113 1103 72-73 0.9444228456586252 1103 74-75 0.829978742867862 1103 76-77 0.5518601318467411 1103 78-79 0.671329752737222 1103 80-81 0.7144724542047953 1103 82-83 0.719859657891611 1103 84-85 0.6317563901979746 1103 86-87 0.7960561022860091 1103 88-89 0.765570390686058 1103 90-91 0.851471240116787 1103 92-93 0.8978194794486676 1103 94-95 0.7627490592664223 1103 96-97 0.6764506522528428 1103 98-99 0.8290120945871671 1103 100 0.55640904614717 1104 1 0.45167915360030264 1104 2 3.631978886947806 1104 3 2.1565224526709876 1104 4 0.9046644724544635 1104 5 0.2870032973412364 1104 6 0.2698871838134522 1104 7 0.18628422237108566 1104 8 0.18646182038131087 1104 9 0.29575546221859383 1104 10-11 0.4155826005912644 1104 12-13 0.5107909143369014 1104 14-15 0.575111605841272 1104 16-17 0.6248231574232292 1104 18-19 0.528054857860802 1104 20-21 0.4918431085500501 1104 22-23 0.4969065753057009 1104 24-25 0.6465933737885052 1104 26-27 0.6520366911725333 1104 28-29 0.6319164715099106 1104 30-31 0.6069712925813846 1104 32-33 0.7403993453111042 1104 34-35 0.7720528185547906 1104 36-37 0.6008304440789729 1104 38-39 0.5577350041384221 1104 40-41 0.5044867224153435 1104 42-43 0.6729403647960908 1104 44-45 0.7905468891491481 1104 46-47 0.7737703385087542 1104 48-49 0.7746630043606615 1104 50-51 0.6576216491358977 1104 52-53 0.5980995155358997 1104 54-55 0.5613421700783832 1104 56-57 0.6308582792587814 1104 58-59 0.8137141372494128 1104 60-61 0.685238200207742 1104 62-63 0.6724326368331504 1104 64-65 0.734786032465145 1104 66-67 0.6168219048465602 1104 68-69 0.6537659946115753 1104 70-71 0.7507233285894301 1104 72-73 0.5748827236953638 1104 74-75 0.7063492044552184 1104 76-77 0.8346326412419565 1104 78-79 0.7074161670511749 1104 80-81 0.6248176636681464 1104 82-83 0.7145773319709292 1104 84-85 0.7339842780380046 1104 86-87 0.641037456304467 1104 88-89 0.5329867485926059 1104 90-91 0.5710147325608901 1104 92-93 0.8209117271019686 1104 94-95 0.7268152193979667 1104 96-97 0.6648119605772074 1104 98-99 0.6282211149569079 1104 100 0.5782557807530999 1105 1 0.48116123106839837 1105 2 3.641028026151073 1105 3 2.1468582007130976 1105 4 1.0095357802732607 1105 5 0.38095364534338216 1105 6 0.3814988514551487 1105 7 0.3583809744312916 1105 8 0.3375057064494271 1105 9 0.4191060331539731 1105 10-11 0.45010986657757 1105 12-13 0.47091180626243556 1105 14-15 0.549866403359637 1105 16-17 0.3736871248162288 1105 18-19 0.39696722776626103 1105 20-21 0.46122981344759495 1105 22-23 0.5077213047885039 1105 24-25 0.5301140697446485 1105 26-27 0.4977442032520969 1105 28-29 0.36511582822956967 1105 30-31 0.24909875212959065 1105 32-33 0.5323242148855343 1105 34-35 0.6607083818945583 1105 36-37 0.43879548015515724 1105 38-39 0.4960214998254102 1105 40-41 0.32869447885477143 1105 42-43 0.45059455827613704 1105 44-45 0.5675363154692334 1105 46-47 0.35726858465496747 1105 48-49 0.3644375673417102 1105 50-51 0.18839865330807015 1105 52-53 0.289373400809545 1105 54-55 0.32080810535070015 1105 56-57 0.37433910724147523 1105 58-59 0.3129067265250285 1105 60-61 0.23688028643908154 1105 62-63 0.20784585778503128 1105 64-65 0.3065679455019321 1105 66-67 0.2130608695311409 1105 68-69 0.16135318774735197 1105 70-71 0.24526856646111383 1105 72-73 0.34073120613636476 1105 74-75 0.3473511641490852 1105 76-77 0.15446599503892244 1105 78-79 0.24597687542994962 1105 80-81 0.22071566810273424 1105 82-83 0.33250895213699394 1105 84-85 0.2805077474183868 1105 86-87 0.19670756808405443 1105 88-89 0.2226822256480574 1105 90-91 0.2092345408768317 1105 92-93 0.266711987591993 1105 94-95 0.15878597566164387 1105 96-97 0.20902394215512388 1105 98-99 0.1702173063135497 1105 100 0.14001382356302017 1106 1 0.4855072677502861 1106 2 3.92691023700743 1106 3 2.2507239631133444 1106 4 0.9329170237802913 1106 5 0.3112546610015485 1106 6 0.3045617490943471 1106 7 0.16687570853333966 1106 8 0.0407117952391971 1106 9 0.2556873151204684 1106 10-11 0.2820678050565064 1106 12-13 0.38056255211807866 1106 14-15 0.39082889834701007 1106 16-17 0.2973046360667979 1106 18-19 0.26315995189229824 1106 20-21 0.33984945872939676 1106 22-23 0.3474834072079318 1106 24-25 0.2850264454842346 1106 26-27 0.3329659197167345 1106 28-29 0.34348794933936233 1106 30-31 0.21049154754634714 1106 32-33 0.4687418096212781 1106 34-35 0.5134613994381638 1106 36-37 0.4102472428414998 1106 38-39 0.39336844171175045 1106 40-41 0.4536050723265177 1106 42-43 0.6081323787032673 1106 44-45 0.5173898060173485 1106 46-47 0.2813806166044728 1106 48-49 0.1605261929552455 1106 50-51 0.284258280954802 1106 52-53 0.2052336499049261 1106 54-55 0.27821923233025103 1106 56-57 0.3538831867118546 1106 58-59 0.34506874639730967 1106 60-61 0.3838710678931818 1106 62-63 0.4588952852314847 1106 64-65 0.3386235569487752 1106 66-67 0.17786635610199397 1106 68-69 0.31056418246386386 1106 70-71 0.2608789462952217 1106 72-73 0.08720595069751624 1106 74-75 0.09181909857100834 1106 76-77 0.14987343084796834 1106 78-79 0.2585612107026698 1106 80-81 0.2628322987722811 1106 82-83 0.33633818977192576 1106 84-85 0.2749701122533068 1106 86-87 0.24881750352374965 1106 88-89 0.38151629416687527 1106 90-91 0.38420165099987713 1106 92-93 0.3833318111005717 1106 94-95 0.34479757681824097 1106 96-97 0.33231290639954736 1106 98-99 0.4421652898885462 1106 100 0.4378442642287439 1107 1 0.42011022508764384 1107 2 3.4718379294978483 1107 3 2.0243350373881057 1107 4 0.9491601236916196 1107 5 0.16597456958077572 1107 6 0.10888203774781857 1107 7 -0.02019352240442629 1107 8 0.08120394294693511 1107 9 -0.09665453490472942 1107 10-11 -0.03558852296048087 1107 12-13 -0.06000818193489721 1107 14-15 0.05444935916395366 1107 16-17 -0.08735246109313266 1107 18-19 0.06644702902223543 1107 20-21 0.0513959146178351 1107 22-23 -0.13646332820113116 1107 24-25 0.09954969793263047 1107 26-27 0.002722803661484363 1107 28-29 0.027358231708227265 1107 30-31 0.04023506662328913 1107 32-33 0.10782378503868983 1107 34-35 0.12102638740517335 1107 36-37 -0.04743043552297621 1107 38-39 -0.09549037270109295 1107 40-41 -0.20081075379874846 1107 42-43 0.11162292700878851 1107 44-45 0.18568840299450784 1107 46-47 0.06133265183513714 1107 48-49 -0.10189771436014894 1107 50-51 0.09811530800395474 1107 52-53 0.10081860214772576 1107 54-55 -0.02070883954252878 1107 56-57 -0.17533602940808635 1107 58-59 -0.05359162452101174 1107 60-61 -0.06594754352126841 1107 62-63 -0.045077274699977465 1107 64-65 -0.1779020617385143 1107 66-67 -0.19922356212899928 1107 68-69 -0.07778180271370161 1107 70-71 -0.23792827672971129 1107 72-73 -0.24559511341810136 1107 74-75 -0.4074062810708021 1107 76-77 -0.19177897534714816 1107 78-79 -0.11280626089266121 1107 80-81 -0.11684439596560736 1107 82-83 0.038640515996934965 1107 84-85 0.08844011740783131 1107 86-87 0.011749512209181745 1107 88-89 0.015500247100398212 1107 90-91 0.09205417389254578 1107 92-93 -0.04760315884288602 1107 94-95 -0.11733265511237434 1107 96-97 -0.0876783528154661 1107 98-99 -0.041551106232759594 1107 100 -0.13590495596177732 1108 1 0.36065373032498727 1108 2 3.4750808429602102 1108 3 2.0098698001767055 1108 4 0.9906759707455421 1108 5 0.21456953117395017 1108 6 0.1120759307510042 1108 7 -0.04260203482116509 1108 8 0.03202885923399634 1108 9 0.018177711481627057 1108 10-11 -0.007691259758708213 1108 12-13 0.0424272105004988 1108 14-15 -0.024078895388356614 1108 16-17 -0.10577056807426288 1108 18-19 0.0903613098864966 1108 20-21 0.015166293823298815 1108 22-23 -0.13192599449953946 1108 24-25 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2210 78-79 0.35616775237388865 2210 80-81 0.193672866546315 2210 82-83 -0.019794837095648887 2210 84-85 0.22361475764665428 2210 86-87 0.4692574947835908 2210 88-89 0.40820696825146996 2210 90-91 0.28318224617094145 2210 92-93 0.3076994103307662 2210 94-95 0.3143601907974478 2210 96-97 0.08241249376329662 2210 98-99 0.3235052651807955 2210 100 0.2915966488628783 2211 1 0.3126635133866529 2211 2 3.807068388587041 2211 3 2.063905709581185 2211 4 0.8011022792873064 2211 5 0.33038436488216405 2211 6 0.24618158323557537 2211 7 0.1597837318108759 2211 8 -0.025955868828781092 2211 9 0.03322057845436177 2211 10-11 0.12053367489018996 2211 12-13 0.15955655501598187 2211 14-15 0.2335260165825872 2211 16-17 0.04545037634711946 2211 18-19 0.053013388302524334 2211 20-21 0.2979189340781616 2211 22-23 -0.06934488380838388 2211 24-25 0.09887010373464022 2211 26-27 0.14440810761625755 2211 28-29 0.1447292152773798 2211 30-31 0.08808728447177572 2211 32-33 0.13381490100319127 2211 34-35 0.08918184054343214 2211 36-37 -0.024492562356954295 2211 38-39 -0.03257927088434087 2211 40-41 0.013437817193072021 2211 42-43 0.0032991532706247995 2211 44-45 -0.023795387832130643 2211 46-47 -0.07594044506576125 2211 48-49 -0.11342503956076655 2211 50-51 -0.04492223598923317 2211 52-53 0.12978137374444998 2211 54-55 0.022643532290537394 2211 56-57 -0.1664903219464975 2211 58-59 -0.2664554550922631 2211 60-61 -0.06821875988281789 2211 62-63 0.03423172683000786 2211 64-65 -0.16391165585132939 2211 66-67 -0.04942145583526525 2211 68-69 -0.14645824818501296 2211 70-71 -0.06238766089005665 2211 72-73 0.04767757644352244 2211 74-75 -0.1535937621029113 2211 76-77 -0.10636236751738082 2211 78-79 0.05791398792129954 2211 80-81 0.2024537033672189 2211 82-83 -0.27813151915009726 2211 84-85 -0.17197244468470885 2211 86-87 -0.15933339604156416 2211 88-89 0.1429918496740754 2211 90-91 0.10684610644596049 2211 92-93 0.04417433412694294 2211 94-95 0.12526297333966951 2211 96-97 0.032442387224957514 2211 98-99 -0.06489019101303128 2211 100 -0.17592794381828725 2212 1 0.8109244749629063 2212 2 4.379866831903733 2212 3 2.2440085265348486 2212 4 1.2891365131278576 2212 5 0.781212383532683 2212 6 0.7171992127290068 2212 7 0.478185108200357 2212 8 0.5053159035062791 2212 9 0.3605060981939303 2212 10-11 0.37217342803055686 2212 12-13 0.45557854756511773 2212 14-15 0.7862186746935436 2212 16-17 0.6752770496648708 2212 18-19 0.5768253660241456 2212 20-21 0.617021834857475 2212 22-23 0.5745431965982633 2212 24-25 0.8872673987775244 2212 26-27 0.7620663216354799 2212 28-29 0.6363102355040908 2212 30-31 0.5220395533294564 2212 32-33 0.8533439383413253 2212 34-35 0.5543151851451356 2212 36-37 0.6560809149366023 2212 38-39 0.5844296379944467 2212 40-41 0.5506797613432113 2212 42-43 0.49100152003939 2212 44-45 0.5147726504143506 2212 46-47 0.6651736938456807 2212 48-49 0.5431770538814646 2212 50-51 0.6978210060840837 2212 52-53 0.5444984072513464 2212 54-55 0.47811150608746544 2212 56-57 0.7117618722926267 2212 58-59 0.6112113338773852 2212 60-61 0.9119461704938132 2212 62-63 0.7798593953367146 2212 64-65 0.8358203769478578 2212 66-67 0.5286703703405848 2212 68-69 0.2935109149740356 2212 70-71 0.32919143546957486 2212 72-73 0.7558571721840828 2212 74-75 0.5807504259633411 2212 76-77 0.6522864456627566 2212 78-79 0.6211947859413662 2212 80-81 0.6990299070552695 2212 82-83 0.5901289339755671 2212 84-85 0.6183583367763603 2212 86-87 0.6901844691255832 2212 88-89 0.7484898495394035 2212 90-91 0.4216539739629752 2212 92-93 0.6302515828870803 2212 94-95 0.6611623331041905 2212 96-97 0.6688995310244046 2212 98-99 0.8132981033551197 2212 100 0.8077040698796445 2213 1 -0.318015685013755 2213 2 -25.61358652325175 2213 3 -14.546953585723378 2213 4 -6.018527909117864 2213 5 -2.1137989100731858 2213 6 -1.3229536853196535 2213 7 -1.298171465150169 2213 8 -1.0734186549276714 2213 9 -1.0497102714212332 2213 10-11 -0.922721604713395 2213 12-13 -0.9204856576031304 2213 14-15 -1.4681652392648417 2213 16-17 -1.354918598918438 2213 18-19 -1.0565517975111263 2213 20-21 -1.1891196129978212 2213 22-23 -1.3312468946300413 2213 24-25 -1.1924206274262161 2213 26-27 -1.1954062272873927 2213 28-29 -1.1925953894194876 2213 30-31 -1.195385418731469 2213 32-33 -0.9307519189538311 2213 34-35 -1.0043868974687271 2213 36-37 -1.084773194841965 2213 38-39 -1.2638831852354144 2213 40-41 -1.224888511882689 2213 42-43 -1.161802147111338 2213 44-45 -1.0713665666052137 2213 46-47 -1.059306296201349 2213 48-49 -1.1283238052048006 2213 50-51 -1.052303942166958 2213 52-53 -1.1980266842879956 2213 54-55 -0.9263497363593345 2213 56-57 -0.8424066802167403 2213 58-59 -0.8595207448968551 2213 60-61 -0.8946864902282456 2213 62-63 -0.9873807820147249 2213 64-65 -0.9734431684078189 2213 66-67 -1.0624335286883522 2213 68-69 -1.0666971962588647 2213 70-71 -0.945537056807229 2213 72-73 -1.1081487223703164 2213 74-75 -1.0594940650521814 2213 76-77 -1.3329707332309617 2213 78-79 -0.8044658310134167 2213 80-81 -0.9322987486731478 2213 82-83 -1.231186486890902 2213 84-85 -1.108561629846907 2213 86-87 -1.1771719690877767 2213 88-89 -1.161838061862099 2213 90-91 -1.3016666250164164 2213 92-93 -1.0805784810563956 2213 94-95 -1.1642114927576337 2213 96-97 -1.1926567342991845 2213 98-99 -0.8016478525043631 2213 100 -0.9889445284476572 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 1.0 4 10.0 5 17.0 6 19.0 7 11.0 8 16.0 9 35.0 10 150.0 11 798.0 12 2247.0 13 4112.0 14 5413.0 15 6050.0 16 6611.0 17 6592.0 18 6893.0 19 7226.0 20 8129.0 21 9467.0 22 10516.0 23 11206.0 24 11204.0 25 11081.0 26 11512.0 27 12624.0 28 14381.0 29 16988.0 30 20391.0 31 25308.0 32 32452.0 33 42804.0 34 57699.0 35 81920.0 36 125572.0 37 194317.0 38 115143.0 39 258.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.52060100950816 19.199201784246977 10.296396290644442 30.983800915600423 2 14.568782895184846 21.33917063585905 32.72178674943883 31.370259719517286 3 16.928312708921105 24.983356145888617 27.461428159073343 30.62690298611693 4 12.555026596673379 18.25032300117561 32.063715619289276 37.13093478286173 5 13.195340708640396 36.81521043408917 32.68289327489982 17.306555582370613 6 32.68939398350711 34.869502127119404 16.506749367920033 15.934354521453454 7 27.828711282709286 31.984714514923375 20.630514615988282 19.55605958637906 8 27.413102686186903 32.75415375622525 19.11117942340542 20.721564134182422 9 26.61815048554994 15.570433851098016 20.16371300156028 37.64770266179176 10-11 25.116484872831645 26.055117445512792 26.171966197117495 22.656431484538064 12-13 26.065303860198224 23.900171864520456 26.515691370444895 23.518832904836426 14-15 24.456346126638994 24.73831080856851 24.3470651627774 26.458277902015087 16-17 22.832683737596312 27.413425237303773 26.157177911610347 23.596713113489567 18-19 23.27713222405853 26.191641843523676 28.106861083734664 22.424364848683126 20-21 23.683093807646387 25.48144284708911 27.91469163966194 22.920771705602565 22-23 23.286153774510232 25.17799296235822 27.3121621975604 24.223691065571145 24-25 23.21267566200485 25.67053220574377 27.10365127843348 24.013140853817898 26-27 23.372684461688088 25.05551122418037 27.603410935551103 23.968393378580437 28-29 23.23767481225109 24.712366689833328 28.424387625976 23.625570871939587 30-31 23.740497962362646 25.386557356246296 27.47489552779202 23.39804915359903 32-33 22.747728742184957 25.29835810654703 27.760121653637476 24.193791497630535 34-35 22.782801203294216 25.330111512480773 27.890749079167982 23.996338205057025 36-37 24.03441619171942 25.052771377573023 27.15024085397969 23.76257157672787 38-39 23.68336663316314 25.130090494445728 27.35337065475051 23.833172217640627 40-41 23.29093119609645 25.205574803387904 27.257394378287902 24.246099622227746 42-43 23.787158432258558 25.15741608888749 27.233812725633605 23.821612753220343 44-45 24.11612597580146 24.868539980433045 26.79636806593866 24.218965977826834 46-47 23.67445650592241 25.212362853234772 27.291289543377665 23.82189109746515 48-49 24.11830792425592 25.311457660269284 26.647573476765103 23.922660938709697 50-51 23.54123834958125 25.49087175284232 26.465513829160564 24.502376068415867 52-53 23.45249245711046 25.561649114616415 25.78163429370437 25.204224134568758 54-55 23.608283068884706 25.859792748647486 25.667350053611532 24.864574128856276 56-57 23.799728765417008 25.206248872281105 25.87863125483828 25.11539110746361 58-59 23.558808259394723 24.613582974602057 26.80147512473139 25.02613364127183 60-61 23.833761124012735 25.363098252169507 26.311396692916134 24.49174393090162 62-63 23.04875990244527 25.232798412116342 26.643209294474424 25.075232390963965 64-65 22.897414405289933 25.357629308839453 26.608400563452427 25.13655572241819 66-67 23.745248022519615 25.038926779237574 26.253442291449964 24.962382906792847 68-69 23.822555041153162 25.045827606701632 26.06116595676808 25.07045139537713 70-71 23.261526854955417 25.067671483906118 26.402240464578565 25.2685611965599 72-73 23.745645157065397 24.97492600041329 26.281218803025762 24.998210039495554 74-75 23.931313973307006 24.768671590496325 26.022350492227737 25.277663943968932 76-77 23.3588436760039 25.089755738662955 26.711820050803375 24.839580534529766 78-79 23.63991835057451 25.287807240304133 26.374778313195506 24.697496095925853 80-81 22.96606243190136 25.219894580978014 26.52057859397845 25.29346439314218 82-83 22.734468485331025 25.124084498517043 26.11600387385751 26.025443142294424 84-85 22.97044824106096 25.182261261900962 25.8177748236754 26.029515673362674 86-87 23.22195579042941 24.692465283032046 26.341826816747954 25.74375210979059 88-89 23.192087584120927 24.453865095730386 26.581659644828253 25.772387675320434 90-91 23.00946452288026 24.71641933505063 26.45136846222164 25.822747679847467 92-93 22.15771300602407 24.975891491049442 26.57709223156041 26.289303271366077 94-95 22.122913220952118 24.819270938208177 26.82140325597474 26.236412584864965 96-97 22.88361374124877 24.66639120541456 26.404823287727037 26.04517176560963 98-99 22.551423325486592 24.56942330802783 26.399159651639525 26.479993714846056 100 22.27695663217156 24.439056445054447 26.9125909079258 26.371396014848198 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 151.0 1 116.0 2 79.0 3 66.0 4 46.0 5 85.5 6 266.0 7 553.0 8 857.0 9 936.0 10 645.0 11 367.0 12 315.0 13 345.5 14 384.5 15 409.0 16 458.5 17 518.0 18 536.0 19 575.5 20 649.0 21 733.5 22 850.5 23 997.0 24 1226.5 25 1551.0 26 2014.5 27 2656.0 28 3524.5 29 4401.5 30 5228.5 31 6315.0 32 7520.5 33 8690.5 34 9889.0 35 11696.0 36 13764.0 37 15745.5 38 17644.5 39 19143.5 40 21082.5 41 22892.5 42 24294.0 43 26164.5 44 27743.0 45 28673.0 46 30246.5 47 32012.0 48 32984.5 49 35694.0 50 46455.0 51 47730.0 52 36651.0 53 32009.5 54 30440.0 55 28885.0 56 27387.0 57 25065.0 58 23009.0 59 22293.5 60 20687.5 61 17955.5 62 15735.0 63 13363.0 64 10620.5 65 8726.5 66 7094.0 67 5474.5 68 4315.5 69 3181.5 70 2238.5 71 1717.0 72 1428.5 73 1099.5 74 800.0 75 536.0 76 278.5 77 155.0 78 93.0 79 46.5 80 23.0 81 8.5 82 4.5 83 2.0 84 1.0 85 1.5 86 1.5 87 0.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.846857919681183 2 5.993766120096464 3 0.0 4 0.005353967056807918 5 0.16864996228944942 6 0.05854446586031267 7 0.03747776939765543 8 0.04364647057180368 9 0.04143504939616563 10-11 0.04347188468951647 12-13 0.04190061174893153 14-15 0.0442866188068568 16-17 0.035906496457070496 18-19 0.031018091753028484 20-21 0.02578051528441204 22-23 0.026013296460794994 24-25 0.020426548227604122 26-27 0.013443112936115534 28-29 0.021299477639040196 30-31 0.025082171755263182 32-33 0.0018040541169678857 34-35 0.004015475292605939 36-37 0.005295771762712179 38-39 0.00727441176196728 40-41 0.007041630585584327 42-43 0.007856364702924662 44-45 0.008671098820264999 46-47 0.01757497881691295 48-49 0.02688622587223107 50-51 0.02257977410914644 52-53 0.029621404694730763 54-55 0.02548953881393335 56-57 0.016411072934998182 58-59 0.015130776464891943 60-61 0.01291935528925389 62-63 0.01990279058074248 64-65 0.021648649403614623 66-67 0.022288797638667746 68-69 0.02915584234196486 70-71 0.028748475283294692 72-73 0.025431343519837613 74-75 0.026071491754890735 76-77 0.020717524698082815 78-79 0.016003705876328017 80-81 0.014897995288508992 82-83 0.009776809408084024 84-85 0.012453792936487984 86-87 0.010009590584466977 88-89 0.008205536467499091 90-91 0.008496512937977783 92-93 0.004597428233563321 94-95 0.0019786399992551005 96-97 0.0012802964701062413 98-99 9.893199996275502E-4 100 0.10358762349041406 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 859176.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.413113810753856 #Duplication Level Percentage of deduplicated Percentage of total 1 77.07240647541414 42.708220316908054 2 11.923888898652717 13.214796252156546 3 4.4297008114405285 7.363905456358282 4 2.1283488534657597 4.717537489843425 5 1.184717282482894 3.282443680389581 6 0.7302067993603217 2.4277819487003893 7 0.47022298230096937 1.8239563744272997 8 0.3529203946006719 1.5645134393714555 9 0.25719533323187344 1.2826794844775309 >10 1.3575762612207125 13.1264260577723 >50 0.07051546616947059 2.630346559237724 >100 0.016566042402852043 1.5409050832422408 >500 0.0031857773650970827 1.1958402572769633 >1k 0.002336236734404527 2.412393818882314 >5k 2.123851576731388E-4 0.7082537809558868 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 6018 0.7004385597363055 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 4142 0.4820898162890956 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2382 0.277242381072097 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1914 0.22277158579848597 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 1864 0.21695205638891216 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 1832 0.2132275575667849 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 1812 0.2108997458029554 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 1600 0.18622494110636237 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 1598 0.18599215992997942 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 1227 0.14281125171094167 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 1125 0.13093941171541104 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 1002 0.11662336936785944 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 980 0.11406277642764695 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 968 0.11266608936934923 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 1.1639058819147648E-4 0.0 2 0.0 0.0 0.0 1.1639058819147648E-4 0.0 3 0.0 0.0 0.0 1.1639058819147648E-4 0.0 4 0.0 0.0 0.0 1.1639058819147648E-4 0.0 5 0.0 0.0 0.0 1.1639058819147648E-4 0.0 6 0.0 0.0 0.0 1.1639058819147648E-4 0.0 7 1.1639058819147648E-4 0.0 0.0 1.1639058819147648E-4 0.0 8 1.1639058819147648E-4 0.0 0.0 1.1639058819147648E-4 0.0 9 1.1639058819147648E-4 0.0 0.0 1.1639058819147648E-4 0.0 10-11 1.1639058819147648E-4 0.0 0.0 2.3278117638295297E-4 0.0 12-13 1.1639058819147648E-4 0.0 0.0 2.3278117638295297E-4 0.0 14-15 1.1639058819147648E-4 0.0 0.0 2.3278117638295297E-4 0.0 16-17 1.1639058819147648E-4 0.0 0.0 2.3278117638295297E-4 0.0 18-19 1.1639058819147648E-4 0.0 0.0 2.3278117638295297E-4 0.0 20-21 1.1639058819147648E-4 0.0 0.0 4.073670586701677E-4 0.0 22-23 1.1639058819147648E-4 0.0 0.0 7.565388232445971E-4 0.0 24-25 1.1639058819147648E-4 0.0 0.0 0.0015130776464891943 0.0 26-27 1.1639058819147648E-4 0.0 0.0 0.0025023976461167443 0.0 28-29 1.1639058819147648E-4 0.0 0.0 0.004481037645371845 0.0 30-31 1.1639058819147648E-4 0.0 0.0 0.01227920705420077 0.0 32-33 1.1639058819147648E-4 0.0 0.0 0.03334590351685801 0.0 34-35 1.1639058819147648E-4 0.0 0.0 0.07338426585472592 0.0 36-37 1.1639058819147648E-4 0.0 0.0 0.12901896701025167 0.0 38-39 1.1639058819147648E-4 1.1639058819147648E-4 0.0 0.20583675521662617 0.0 40-41 1.1639058819147648E-4 1.1639058819147648E-4 0.0 0.2954575081240631 0.0 42-43 1.1639058819147648E-4 1.1639058819147648E-4 0.0 0.3824594727971917 0.0 44-45 1.1639058819147648E-4 1.1639058819147648E-4 0.0 0.5004213339292531 0.0 46-47 1.1639058819147648E-4 1.1639058819147648E-4 0.0 0.6288001527044518 0.0 48-49 1.1639058819147648E-4 1.1639058819147648E-4 0.0 0.7461800608955558 0.0 50-51 1.1639058819147648E-4 1.1639058819147648E-4 0.0 0.8711835526132015 0.0 52-53 1.1639058819147648E-4 1.1639058819147648E-4 0.0 1.0056146819743568 0.0 54-55 1.1639058819147648E-4 1.1639058819147648E-4 0.0 1.1573298136819465 0.0 56-57 1.1639058819147648E-4 1.1639058819147648E-4 0.0 1.347744815963202 0.0 58-59 1.1639058819147648E-4 1.1639058819147648E-4 0.0 1.5461907688296694 0.0 60-61 1.1639058819147648E-4 1.1639058819147648E-4 0.0 1.751911133458104 0.0 62-63 1.1639058819147648E-4 1.1639058819147648E-4 0.0 1.9650223004366976 0.0 64-65 1.1639058819147648E-4 1.1639058819147648E-4 0.0 2.224340530927307 0.0 66-67 1.1639058819147648E-4 1.1639058819147648E-4 0.0 2.5081589802322224 0.0 68-69 1.1639058819147648E-4 2.3278117638295297E-4 0.0 2.83510014246208 0.0 70-71 1.1639058819147648E-4 2.3278117638295297E-4 0.0 3.2182579587884206 0.0 72-73 1.1639058819147648E-4 2.3278117638295297E-4 0.0 3.587157928061305 0.0 74-75 1.1639058819147648E-4 2.3278117638295297E-4 0.0 3.9739238526215814 0.0 76-77 1.1639058819147648E-4 2.3278117638295297E-4 0.0 4.416149892455096 0.0 78-79 1.1639058819147648E-4 2.3278117638295297E-4 0.0 4.8961446781567455 0.0 80-81 1.1639058819147648E-4 2.3278117638295297E-4 0.0 5.431483188543441 0.0 82-83 1.1639058819147648E-4 2.3278117638295297E-4 0.0 6.012912371853963 0.0 84-85 1.1639058819147648E-4 2.3278117638295297E-4 0.0 6.605689637513152 0.0 86-87 1.1639058819147648E-4 2.3278117638295297E-4 0.0 7.217438569047553 0.0 88 1.1639058819147648E-4 2.3278117638295297E-4 0.0 7.737762693557548 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCTAAGG 170 5.361208E-5 20.586857 2 TTAGACC 115 0.003954931 20.437368 4 TCTAGGG 350 3.6379788E-12 20.145405 3 CGATTCG 120 0.005097044 19.560722 9 GTACTTT 2835 0.0 19.3733 14-15 GAGTACT 2840 0.0 19.1739 12-13 CCTAGGT 125 0.0064051924 18.80238 3 TATACTG 125 0.0064051924 18.80238 5 CAGAACG 150 8.2810316E-4 18.802378 4 GGTCTAG 240 1.8737355E-6 18.76853 1 CATTACG 130 0.008031444 18.07921 4 ATTACGA 135 0.009981551 17.409609 5 AGTACTT 2910 0.0 16.857536 12-13 CATGGGG 2695 0.0 16.569813 4 CACACCG 200 2.9883196E-4 16.452082 5 TACTTTT 3035 0.0 16.395248 14-15 ACTTTTT 3500 0.0 16.227448 16-17 TAATAGA 290 1.1471275E-6 16.208948 4 ATTAGAC 205 3.6068982E-4 16.050812 3 GCATATA 220 3.8295618E-4 15.924814 1 >>END_MODULE