FastQCFastQC Report
Wed 25 May 2016
SRR1295131_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295131_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences618693
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT62231.0058300320191111No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA51220.8278742445768742No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG23280.3762770873438038No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC22240.3594674580122937No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC20070.3243935198878927No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG16950.2739646318933623No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC16770.27105527297060095No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG14760.23856743166643232No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT14700.23759764535884517No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG13430.21707050184825108No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT10590.17116728328912725No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9630.15565070236773326No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC8560.13835617988242957No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC8120.13124441362679068No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC7660.12380938526862272No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA7500.1212232884483904No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT7370.11912208478195165No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC6910.11168705642378368No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC6870.11104053221872562No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG6680.10796954224469972No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT6660.10764628014217067No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG6260.10118103809158986No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC859.094947E-1266.470481
TCTCGCT1201.2927558E-841.8416022
CTCGCTC1159.699634E-734.508833
GTACAAA2459.094947E-1230.7482551
TAGGACC750.00495041630.2363074
TCGCTCT1205.1320538E-528.3465394
TGTACTG1158.747735E-526.211795
GTATAAC1209.409528E-426.1573661
GTACAAG3253.092282E-1023.1794531
ATGGGTC1854.0246396E-621.7250865
CGCTCTG1403.3195366E-421.5311155
CATGGGT7200.021.2599034
CATATAC2801.1078373E-720.2475283
CATAGAC1400.004171561420.2475283
ACGTCAT1009.942916E-619.57251426-27
CTGTTGC3404.0563464E-1019.2560089
TTAGGAC1807.996374E-418.8976923
ATACTAC1500.006216511618.8976923
GTACATA3352.5014924E-718.7396071
GTCATCG1051.620987E-518.49402428-29
Produced by FastQC (version 0.11.5)