##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295131_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 618693 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.962420780581 27.0 27.0 33.0 6.0 33.0 2 21.828155482606075 27.0 2.0 33.0 2.0 33.0 3 24.323077843130598 27.0 15.0 33.0 6.0 33.0 4 24.104985833038356 33.0 2.0 37.0 2.0 37.0 5 26.19998448341908 33.0 15.0 37.0 2.0 37.0 6 27.001066764938347 33.0 15.0 37.0 2.0 37.0 7 26.833607297965226 33.0 15.0 37.0 6.0 37.0 8 27.59960755980753 33.0 15.0 37.0 6.0 37.0 9 28.334257862946565 33.0 22.0 37.0 6.0 37.0 10-11 27.969244035410128 33.0 18.5 37.0 6.0 37.0 12-13 26.768006103188497 33.0 15.0 37.0 4.0 37.0 14-15 28.48072711344722 33.0 18.5 37.0 6.0 40.0 16-17 28.91008787880257 33.0 22.0 37.0 6.0 40.0 18-19 29.1185402129974 33.0 22.0 38.5 6.0 40.0 20-21 28.637194860779097 33.0 18.5 37.0 6.0 40.0 22-23 29.520671803301475 33.0 24.5 37.0 6.0 40.0 24-25 29.805268525747017 33.0 24.5 37.0 10.5 40.0 26-27 28.26015729933909 33.0 18.5 37.0 4.0 40.0 28-29 29.025350214080326 33.0 22.0 37.0 6.0 40.0 30-31 29.7673288690837 33.0 27.0 37.0 15.0 40.0 32-33 28.444583985918705 33.0 22.0 37.0 4.0 40.0 34-35 28.140775796719858 33.0 22.0 37.0 6.0 40.0 36-37 28.47387476502886 33.0 22.0 37.0 6.0 40.0 38-39 28.51799519309254 33.0 22.0 37.0 6.0 40.0 40-41 27.547129998238223 33.0 15.0 37.0 6.0 40.0 42-43 28.062257048326067 33.0 22.0 37.0 6.0 40.0 44-45 27.93965747147616 33.0 22.0 37.0 6.0 40.0 46-47 27.51677487865549 33.0 22.0 37.0 6.0 40.0 48-49 27.46945981286357 33.0 18.5 37.0 6.0 40.0 50-51 27.427461600502994 33.0 22.0 37.0 6.0 40.0 52-53 27.75350294895853 33.0 22.0 37.0 6.0 40.0 54-55 27.704907765240595 33.0 22.0 37.0 6.0 40.0 56-57 27.63735164289882 33.0 22.0 37.0 6.0 40.0 58-59 27.467241426684964 33.0 22.0 37.0 6.0 40.0 60-61 27.386339428440277 33.0 22.0 37.0 6.0 40.0 62-63 27.23897959084716 33.0 22.0 37.0 6.0 40.0 64-65 26.877089283376407 33.0 22.0 37.0 6.0 37.0 66-67 27.11411394665852 33.0 22.0 37.0 6.0 37.0 68-69 26.592077977284372 30.0 18.5 37.0 6.0 37.0 70-71 26.597092580649853 27.0 22.0 37.0 6.0 37.0 72-73 26.56890412530932 27.0 22.0 33.0 6.0 37.0 74-75 26.665550604257685 27.0 22.0 33.0 6.0 37.0 76-77 26.31970217862494 27.0 22.0 33.0 6.0 37.0 78-79 25.8769729090195 27.0 22.0 33.0 6.0 37.0 80-81 25.76472014391629 27.0 22.0 33.0 6.0 37.0 82-83 25.90784282350051 27.0 22.0 33.0 6.0 37.0 84-85 25.7810190191258 27.0 22.0 33.0 6.0 37.0 86-87 25.481769633727872 27.0 22.0 33.0 6.0 37.0 88-89 25.388304862023652 27.0 22.0 33.0 6.0 37.0 90-91 25.523753299293837 27.0 22.0 33.0 6.0 37.0 92-93 25.288738518134195 27.0 22.0 33.0 6.0 37.0 94-95 25.196421326893955 27.0 18.5 33.0 6.0 37.0 96-97 25.34537484665254 27.0 22.0 33.0 6.0 37.0 98-99 25.374891909234467 27.0 22.0 33.0 6.0 37.0 100 24.868587490079893 27.0 15.0 33.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.2702200178456415 1101 2 11.182483919191615 1101 3 8.395402867645583 1101 4 12.5910947931486 1101 5 11.036064612063605 1101 6 10.087369713322836 1101 7 9.383426858049578 1101 8 8.996107198375757 1101 9 7.207301512628817 1101 10-11 7.8083978552088915 1101 12-13 9.057651582930301 1101 14-15 7.7610040789538886 1101 16-17 7.879525005496454 1101 18-19 7.946910553836894 1101 20-21 6.8824150772171 1101 22-23 7.369049684855629 1101 24-25 7.263583076271939 1101 26-27 8.551473860709056 1101 28-29 8.102461224712261 1101 30-31 5.179048274569105 1101 32-33 7.020993531181048 1101 34-35 8.80603287026192 1101 36-37 8.234639385011643 1101 38-39 8.316611004616359 1101 40-41 9.246729779308811 1101 42-43 9.043611287669211 1101 44-45 8.465055409226753 1101 46-47 7.333974073234593 1101 48-49 5.799189173620693 1101 50-51 6.9439234020542 1101 52-53 6.016544601913271 1101 54-55 3.962003876604207 1101 56-57 6.1670770851949825 1101 58-59 6.143772584917301 1101 60-61 6.7793280623285845 1101 62-63 6.322647495247082 1101 64-65 6.61968992508627 1101 66-67 6.0064705724437175 1101 68-69 7.071276079555528 1101 70-71 6.275659152382982 1101 72-73 5.418043912948246 1101 74-75 5.117548187509193 1101 76-77 5.159825800411035 1101 78-79 5.103918356744892 1101 80-81 5.027870700149549 1101 82-83 4.689026896618191 1101 84-85 4.47105779350731 1101 86-87 4.337755020516919 1101 88-89 4.164034684588373 1101 90-91 3.9913482235248203 1101 92-93 4.012154842951677 1101 94-95 3.870832156727694 1101 96-97 4.1330077027359025 1101 98-99 3.621191439372396 1101 100 4.0670262214223705 1102 1 6.64912574846657 1102 2 10.19018419749283 1102 3 8.093585886228038 1102 4 12.235563843027329 1102 5 10.322275864235685 1102 6 9.066254124328346 1102 7 6.384335807244703 1102 8 7.631895955373405 1102 9 6.080721454034261 1102 10-11 7.164921428284739 1102 12-13 8.714920425573201 1102 14-15 5.756546445385354 1102 16-17 7.068482705545513 1102 18-19 7.048869322159156 1102 20-21 6.648803671910574 1102 22-23 6.272757234750397 1102 24-25 6.687470329892829 1102 26-27 8.326219515404642 1102 28-29 7.533691137278918 1102 30-31 3.731856159617408 1102 32-33 3.8913777198206745 1102 34-35 4.526778002283059 1102 36-37 4.768467654363199 1102 38-39 4.429927053014179 1102 40-41 6.349654807620347 1102 42-43 6.913005718893967 1102 44-45 6.006397123690622 1102 46-47 5.410921837884377 1102 48-49 3.347479821874547 1102 50-51 4.958788102731379 1102 52-53 5.692964419527396 1102 54-55 5.30391377028122 1102 56-57 6.068368412008638 1102 58-59 5.823726759206302 1102 60-61 5.682028661570243 1102 62-63 4.179792317331454 1102 64-65 5.801687229186513 1102 66-67 3.3835767441285185 1102 68-69 5.917473966850412 1102 70-71 4.848224933712501 1102 72-73 4.650277566763542 1102 74-75 3.7871044644166147 1102 76-77 2.9940973114445057 1102 78-79 3.9181511486058405 1102 80-81 4.156701353630147 1102 82-83 3.6171327977960424 1102 84-85 2.7830216510280934 1102 86-87 3.312514773667864 1102 88-89 3.5248462512820886 1102 90-91 3.2223296364880625 1102 92-93 3.0388330176258087 1102 94-95 3.0368810130334865 1102 96-97 3.2871766045235375 1102 98-99 3.1536408110168495 1102 100 2.92953533383864 1103 1 6.542423093137263 1103 2 10.675553314372074 1103 3 7.81493014668899 1103 4 11.703584280112437 1103 5 10.236639629432965 1103 6 8.828293077860081 1103 7 7.794254599666573 1103 8 7.663187911068189 1103 9 6.751495117046119 1103 10-11 7.2057454847477835 1103 12-13 8.605481326595058 1103 14-15 8.10360278722171 1103 16-17 7.658794669444966 1103 18-19 7.04625874559467 1103 20-21 7.951821684888873 1103 22-23 6.544972775139751 1103 24-25 5.100496174600817 1103 26-27 5.693039320357279 1103 28-29 6.870785332475453 1103 30-31 6.0358904327583005 1103 32-33 7.903620624646514 1103 34-35 7.061724765168119 1103 36-37 4.102821909603001 1103 38-39 6.209340325931311 1103 40-41 6.61817591599657 1103 42-43 7.260036421139066 1103 44-45 5.465426222295829 1103 46-47 6.149266312785549 1103 48-49 5.507726101802945 1103 50-51 6.152376340219259 1103 52-53 6.0749010943987365 1103 54-55 4.639618765805949 1103 56-57 5.636634989169824 1103 58-59 5.287691479128732 1103 60-61 4.692558375525639 1103 62-63 2.433435470162067 1103 64-65 4.986603457649256 1103 66-67 3.9285392637078402 1103 68-69 4.9635483123415725 1103 70-71 3.636768231989521 1103 72-73 4.101872349339807 1103 74-75 3.2830662287934302 1103 76-77 3.475198601815208 1103 78-79 3.1073002404981374 1103 80-81 4.0585704798328734 1103 82-83 3.4485594778885336 1103 84-85 3.2618666573680635 1103 86-87 2.674548133106967 1103 88-89 3.013082340638398 1103 90-91 2.590835827283918 1103 92-93 2.2790899729476557 1103 94-95 2.965409486965015 1103 96-97 2.7896787955431535 1103 98-99 2.8081275063998596 1103 100 2.83020522643206 1104 1 6.6864791456007175 1104 2 10.09734082047903 1104 3 7.730712870579403 1104 4 12.49202991880611 1104 5 9.875732605493695 1104 6 8.249622562245065 1104 7 4.232923142264941 1104 8 7.193808424804143 1104 9 6.9492595012770195 1104 10-11 7.556174968193236 1104 12-13 7.860883634085003 1104 14-15 7.552698370483007 1104 16-17 7.484527973965989 1104 18-19 7.13847848327741 1104 20-21 7.94818804589455 1104 22-23 6.817929058728009 1104 24-25 6.538414266223949 1104 26-27 8.696081641564387 1104 28-29 7.5998167457190995 1104 30-31 6.800436732882325 1104 32-33 8.012982706085118 1104 34-35 8.132998817180596 1104 36-37 6.96709480599144 1104 38-39 7.850593050573348 1104 40-41 7.405510558410789 1104 42-43 7.533952788721461 1104 44-45 7.14016796850952 1104 46-47 7.065374936747364 1104 48-49 5.9444691915470145 1104 50-51 5.725183003378344 1104 52-53 6.220505025399522 1104 54-55 5.182871870958337 1104 56-57 6.353488309474983 1104 58-59 4.034393722730517 1104 60-61 4.653109199494974 1104 62-63 4.053868171503716 1104 64-65 3.0872995332251882 1104 66-67 3.3866760783194394 1104 68-69 4.372501814915889 1104 70-71 2.5439706822882897 1104 72-73 2.895862399698707 1104 74-75 3.384173349504465 1104 76-77 4.17609867321962 1104 78-79 4.352158065857974 1104 80-81 3.2525174291733236 1104 82-83 3.3897988142033135 1104 84-85 3.173343977071923 1104 86-87 0.8882317082131337 1104 88-89 0.753340570936416 1104 90-91 2.344093653583286 1104 92-93 2.8038231459342384 1104 94-95 2.5268090604176088 1104 96-97 3.0805454900825993 1104 98-99 2.94103190434792 1104 100 1.4921722770540313 1105 1 5.749185320424193 1105 2 -2.534706279981595 1105 3 0.6587082910454143 1105 4 8.536395315457153 1105 5 8.775602906217205 1105 6 7.82946193021364 1105 7 5.387059179040548 1105 8 6.04298221602895 1105 9 4.749155114840519 1105 10-11 4.92098059925511 1105 12-13 4.995798748985557 1105 14-15 5.698317449397528 1105 16-17 5.954088703300904 1105 18-19 5.854337209762882 1105 20-21 6.897415772046781 1105 22-23 1.8539156731124393 1105 24-25 4.185474899764863 1105 26-27 6.788085396689372 1105 28-29 5.854731849008257 1105 30-31 5.163368284872924 1105 32-33 7.140471753637264 1105 34-35 6.090861066563161 1105 36-37 6.131275279573774 1105 38-39 3.83690031361672 1105 40-41 5.029985287952382 1105 42-43 5.388944178872443 1105 44-45 6.0378924835305945 1105 46-47 5.265283380901785 1105 48-49 4.861349314349123 1105 50-51 5.781747331084407 1105 52-53 5.3291583590889395 1105 54-55 5.139153809550319 1105 56-57 4.7501222242682495 1105 58-59 1.4974668756769667 1105 60-61 4.020100385285414 1105 62-63 -0.7673032904753541 1105 64-65 -0.43812216359433265 1105 66-67 0.9368596544663816 1105 68-69 2.691407893829247 1105 70-71 2.705385321702238 1105 72-73 2.5734792887042275 1105 74-75 2.6128080933512976 1105 76-77 1.829325828091239 1105 78-79 3.0120864467767383 1105 80-81 1.70771747608336 1105 82-83 1.060200672955343 1105 84-85 1.240095561736986 1105 86-87 -0.32044159707481157 1105 88-89 1.3874591441747093 1105 90-91 1.315890737497675 1105 92-93 1.7558875357612642 1105 94-95 0.8304676366516368 1105 96-97 2.102243412688008 1105 98-99 1.8187772734335113 1105 100 1.8174213704944044 1106 1 6.217926853827205 1106 2 10.141995823728596 1106 3 6.789759062416991 1106 4 11.641374693969134 1106 5 9.738223910472222 1106 6 8.95665430749721 1106 7 6.1134595437082275 1106 8 6.8967693580298075 1106 9 6.522392484563163 1106 10-11 5.534548493751746 1106 12-13 7.539615011890394 1106 14-15 7.7226981763084765 1106 16-17 7.3995726169761085 1106 18-19 6.850772929543034 1106 20-21 7.950498437316146 1106 22-23 5.866163155548005 1106 24-25 5.090729454898781 1106 26-27 7.602437259661915 1106 28-29 6.088541201773399 1106 30-31 5.132729328778844 1106 32-33 7.111090532346886 1106 34-35 6.544356999524194 1106 36-37 5.452415782209631 1106 38-39 6.5791484719834905 1106 40-41 6.249726612989825 1106 42-43 5.567930762475918 1106 44-45 4.213512259378401 1106 46-47 5.539077136973294 1106 48-49 5.425494627268961 1106 50-51 6.377060614026416 1106 52-53 5.275458417799989 1106 54-55 4.936835030337939 1106 56-57 4.607137077437557 1106 58-59 4.660845034607949 1106 60-61 4.549043927153765 1106 62-63 4.418111901665071 1106 64-65 4.823835813594943 1106 66-67 2.4993335738562763 1106 68-69 3.032175818714858 1106 70-71 2.7222651673888194 1106 72-73 2.616398127242139 1106 74-75 2.824775930198264 1106 76-77 3.4868513165632606 1106 78-79 3.7367589972104263 1106 80-81 3.3713685212908686 1106 82-83 1.74836036924437 1106 84-85 2.355025094512108 1106 86-87 3.049912562946595 1106 88-89 3.0884481264960364 1106 90-91 2.664543316119371 1106 92-93 2.353677743473245 1106 94-95 0.9989647175784775 1106 96-97 1.3191628645915898 1106 98-99 1.7063925378594753 1106 100 2.3661244484393293 1107 1 6.123206729237719 1107 2 10.24585394041246 1107 3 6.1915359583702525 1107 4 11.232172251452088 1107 5 9.534777792565343 1107 6 7.88595076404377 1107 7 3.6455632231014725 1107 8 6.489871073114944 1107 9 5.8556105183633385 1107 10-11 4.799648149942836 1107 12-13 6.094118512670182 1107 14-15 6.292065143377169 1107 16-17 7.192290544092138 1107 18-19 6.350241158564202 1107 20-21 5.506070979236416 1107 22-23 5.76531501658145 1107 24-25 4.580570539513609 1107 26-27 7.20468824016621 1107 28-29 6.431076775987069 1107 30-31 5.012590331433849 1107 32-33 5.027599464119625 1107 34-35 6.5764824006895815 1107 36-37 6.21596604364078 1107 38-39 6.417985020872326 1107 40-41 7.462412182914132 1107 42-43 5.818081990003506 1107 44-45 3.736710012192333 1107 46-47 3.2874645016109056 1107 48-49 3.7235415374981002 1107 50-51 4.613741868192047 1107 52-53 3.7667842817766015 1107 54-55 5.383624112480334 1107 56-57 5.0733072045566665 1107 58-59 2.606528781003231 1107 60-61 2.599053770691018 1107 62-63 0.7345387306382882 1107 64-65 1.8841063073995663 1107 66-67 3.8849923649599205 1107 68-69 4.823627833945395 1107 70-71 4.14398759943542 1107 72-73 2.149849931304054 1107 74-75 2.163601755680599 1107 76-77 2.667353193984276 1107 78-79 3.5688455741450937 1107 80-81 1.8369854306181423 1107 82-83 1.5368791203579555 1107 84-85 2.098263849233156 1107 86-87 1.8840115658347614 1107 88-89 2.3739986936817523 1107 90-91 1.1672860335515551 1107 92-93 2.0957108023606956 1107 94-95 2.090511263761716 1107 96-97 2.2006252292841992 1107 98-99 1.7551730390769968 1107 100 -0.1796716462609993 1108 1 4.237603462782609 1108 2 -3.114710758957381 1108 3 0.16268842226523716 1108 4 6.421972196095716 1108 5 7.100004442582183 1108 6 6.633071374422876 1108 7 4.213693937091399 1108 8 5.322627777565554 1108 9 5.064656457715927 1108 10-11 3.3946009575622753 1108 12-13 2.4687513475815983 1108 14-15 4.1502275800016335 1108 16-17 4.735185027979789 1108 18-19 4.061815682617404 1108 20-21 4.685494285985815 1108 22-23 3.9963669914951474 1108 24-25 3.002590199608317 1108 26-27 4.849821975155084 1108 28-29 6.14487494193175 1108 30-31 4.515021827922581 1108 32-33 4.424584848320745 1108 34-35 2.976843343550218 1108 36-37 0.9870972124970265 1108 38-39 2.380625906059347 1108 40-41 5.1771994685422875 1108 42-43 3.0466501695298014 1108 44-45 3.7491598320695623 1108 46-47 4.710017342272522 1108 48-49 4.599329775770812 1108 50-51 1.8726678150971345 1108 52-53 -0.0017989920164467321 1108 54-55 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-16.75254493998607 2213 48-49 -6.226285816157144 2213 50-51 -23.169270001244154 2213 52-53 -17.70271531358353 2213 54-55 -17.472120424881414 2213 56-57 -17.436557915486496 2213 58-59 -17.770845106237125 2213 60-61 -18.926043342877907 2213 62-63 -17.19525355422818 2213 64-65 -17.463517107760943 2213 66-67 -8.803262288398589 2213 68-69 -22.34514071204867 2213 70-71 -16.905936867788753 2213 72-73 -9.000270111857532 2213 74-75 -4.086982274532513 2213 76-77 -3.014224901510733 2213 78-79 -4.595946710721375 2213 80-81 -3.1347707064198964 2213 82-83 -2.9667343136857447 2213 84-85 -2.7299071785521143 2213 86-87 -2.7046342393985796 2213 88-89 -2.260989167122137 2213 90-91 -2.450821600653999 2213 92-93 -3.1905220004448047 2213 94-95 -3.359415901061592 2213 96-97 -2.963013831951301 2213 98-99 -3.681541285174422 2213 100 -2.6407398955191574 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 188.0 3 245.0 4 1477.0 5 4371.0 6 5286.0 7 5642.0 8 5295.0 9 5441.0 10 6519.0 11 8229.0 12 9948.0 13 12347.0 14 11051.0 15 12546.0 16 13525.0 17 13818.0 18 13549.0 19 13823.0 20 14120.0 21 14180.0 22 15230.0 23 16440.0 24 17221.0 25 18517.0 26 19633.0 27 20480.0 28 21560.0 29 22358.0 30 23946.0 31 26015.0 32 29899.0 33 35070.0 34 41035.0 35 46146.0 36 46405.0 37 36306.0 38 10804.0 39 28.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.454399824212594 21.41168631870683 12.914349710624151 22.21956414645643 2 14.29622032122905 24.028456703910614 37.2226344273743 24.452688547486034 3 19.647732413922213 31.028738531195664 28.28350589626116 21.04002315862095 4 13.044571190818784 21.313471906800963 33.85247819981624 31.78947870256401 5 11.745482506728182 41.76231695800346 32.98078035706954 13.511420178198827 6 29.529968380118344 35.81869098390441 18.90666440078607 15.744676235191173 7 23.835939347102684 35.59599552549024 22.42036395972941 18.147701167677667 8 25.10919369872896 36.31123507626595 21.284467888302792 17.295103336702297 9 25.580317676369553 19.87435213890347 20.902722085330424 33.642608099396554 10-11 21.675889465949773 31.830213279958837 25.132237063007203 21.36166019108419 12-13 24.47382855174227 29.866774250576828 25.31443362057633 20.344963577104572 14-15 24.068772445975693 29.599654413356113 23.936010163485005 22.395562977183186 16-17 20.175260806879443 32.78196659937214 27.24100971891183 19.801762874836594 18-19 21.693091801631574 31.218482355333904 28.33724162267306 18.751184220361463 20-21 22.74739663974095 30.38504074247746 26.89481274385766 19.972749873923927 22-23 20.72121274870149 31.017066142085554 25.305085330710426 22.95663577850253 24-25 21.474295355299724 31.46115385141887 24.20877195384047 22.855778839440937 26-27 22.67181144117679 28.847714191536294 25.19659655236115 23.283877814925763 28-29 22.284214808221844 28.189320084554836 27.596271521152616 21.930193586070704 30-31 23.906919935368133 30.329223866291766 25.765552572763863 19.998303625576234 32-33 19.912414297909503 30.20615032741733 27.6719097584503 22.209525616222862 34-35 18.828396202049564 30.049597485543188 28.088373701513312 23.033632610893935 36-37 22.874243131982066 29.88872348506345 25.98570736328355 21.25132601967093 38-39 23.708262542907665 28.12359141499948 25.930272875420407 22.237873166672443 40-41 21.25418392075091 30.175491057244848 25.8747631596044 22.695561862399845 42-43 23.061010564957744 28.942851799174463 26.250592620622765 21.74554501524503 44-45 24.441337258017224 27.934746764789566 25.805202613400947 21.81871336379227 46-47 21.68579017517301 29.35185032827078 28.69331689020488 20.26904260635133 48-49 22.51710991298327 28.63749783619237 28.29010643822488 20.555285812599482 50-51 21.261664923995404 29.159671999909303 27.746494963403904 21.832168112691384 52-53 20.00207272724184 29.69878346201515 25.805709004385857 24.493434806357158 54-55 21.068008537107584 30.694518610519207 24.41077067698035 23.82670217539286 56-57 22.602271324002196 28.10565446408421 25.19968829974323 24.09238591217036 58-59 21.974916077664005 26.98933891128692 27.60471314586461 23.43103186518446 60-61 23.549564367488017 28.86201963755307 25.90817119040702 21.680244804551887 62-63 20.116372050007765 29.365537780433936 27.320805239162844 23.19728493039546 64-65 18.901038846864708 29.43062841664531 27.760343917543974 23.907988818946013 66-67 22.046049497765026 28.713059771905602 26.176820960277592 23.06406977005178 68-69 23.867418936112788 27.41353827963346 25.703196288347108 23.015846495906644 70-71 21.62552012018707 29.200765976278692 25.5850052323966 23.588708671137635 72-73 23.15814837445343 28.234337260719855 26.27412538415169 22.33338898067502 74-75 24.052005924147423 27.360280157368322 25.5341845489362 23.053529369548055 76-77 21.81689982827142 28.570001302090798 27.882513739514685 21.730585130123092 78-79 22.966419662763048 28.212327785220303 27.91539580768958 20.905856744327068 80-81 21.608423016891194 28.43338933127539 27.537008684230337 22.42117896760308 82-83 20.501493834005196 29.075341654820235 26.084652498854823 24.33851201231974 84-85 21.41088692503822 29.174014694140148 24.78489324878691 24.630205132034718 86-87 22.68441081302179 27.530695571511004 25.65532174250374 24.129571872963467 88-89 22.32004716443385 26.623150310575188 27.56953333919632 23.487269185794645 90-91 23.471652894706118 27.829278496283514 26.21718079103374 22.481887817976627 92-93 20.826626600788494 28.47567028769346 27.14771695112953 23.549986160388517 94-95 19.875115340112973 28.484654679431788 27.47853087209331 24.161699108361933 96-97 22.399660802322952 27.986005654379703 26.34918154725574 23.26515199604161 98-99 23.373643315144022 26.938266270064965 25.928134082132942 23.75995633265807 100 21.63114856764543 28.01822508731092 26.711367255679885 23.639259089363765 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1144.0 1 1184.0 2 1108.0 3 820.0 4 567.5 5 447.0 6 404.5 7 437.0 8 537.5 9 689.0 10 845.0 11 1016.0 12 1227.0 13 1420.0 14 1683.5 15 1883.0 16 2045.5 17 2307.5 18 2507.0 19 2674.5 20 2902.0 21 3183.0 22 3532.5 23 3971.0 24 4519.0 25 5215.5 26 6107.0 27 7240.0 28 8429.5 29 9725.0 30 11175.0 31 12283.5 32 13171.5 33 14095.5 34 14778.5 35 15328.0 36 15813.0 37 16187.5 38 16578.5 39 16769.0 40 16721.5 41 16517.0 42 16170.0 43 15846.0 44 15735.0 45 16026.0 46 17129.0 47 19518.5 48 23200.5 49 30201.0 50 39696.0 51 38320.0 52 26379.0 53 17610.0 54 13530.0 55 11428.5 56 9982.5 57 8816.5 58 7675.5 59 6637.0 60 5705.0 61 4750.0 62 3798.5 63 2986.5 64 2281.0 65 1708.0 66 1257.0 67 883.0 68 623.0 69 485.5 70 397.0 71 303.0 72 227.0 73 182.0 74 154.0 75 111.5 76 76.0 77 59.5 78 40.5 79 35.5 80 31.0 81 21.5 82 16.0 83 13.0 84 10.0 85 5.5 86 4.5 87 3.5 88 1.5 89 0.5 90 0.0 91 0.0 92 1.0 93 1.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 8.788688412508304 2 25.93418706854611 3 6.756501204959488 4 25.413088559269298 5 13.397112946162313 6 10.596531720901966 7 8.393015599012758 8 9.4843484571508 9 5.4767065410470135 10-11 7.335706077165896 12-13 10.859262994732443 14-15 8.143618886911602 16-17 8.689851024660049 18-19 7.018020245905482 20-21 8.65663584362519 22-23 6.333189481697708 24-25 5.541520592604086 26-27 10.756061568500048 28-29 7.022061022187094 30-31 4.719707512449632 32-33 9.685902378077657 34-35 7.692910700460487 36-37 7.438341794718867 38-39 6.767977009599269 40-41 9.191877069887651 42-43 7.098108431807051 44-45 7.118150682163852 46-47 6.683363154262293 48-49 4.29380969236762 50-51 7.3282710488077285 52-53 4.864528934382642 54-55 5.260444194455085 56-57 5.106005724971836 58-59 4.183496499879585 60-61 4.581351332567203 62-63 3.178636254168061 64-65 4.765206653380594 66-67 2.7851454598645855 68-69 5.677371491191916 70-71 2.849555433793497 72-73 1.7290481709022083 74-75 0.9065885665426956 76-77 1.3151110486137711 78-79 1.9104790259466327 80-81 2.296211529789411 82-83 1.0251449426452215 84-85 1.5197359595146542 86-87 0.42064481091591466 88-89 0.7549786404565755 90-91 0.3201911125550152 92-93 0.4376160712986893 94-95 0.6798202016185734 96-97 0.6953367825399673 98-99 0.5059051904579492 100 0.6361798177771527 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 618693.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.00691447544767 #Duplication Level Percentage of deduplicated Percentage of total 1 95.66214985838094 79.40619891832085 2 2.9588129901848204 4.912038736502301 3 0.6752827001921454 1.681594000247964 4 0.2346361110325281 0.7790567840531482 5 0.10991560817421528 0.4561877743616953 6 0.06463159662390151 0.3218921648023096 7 0.04094090184850728 0.23788645568007194 8 0.029181874521158245 0.19378378900888224 9 0.01950565472197325 0.1457193791965001 >10 0.16925268204233704 2.6821798482196417 >50 0.019141049496948257 1.1234410060094462 >100 0.011165571996656723 1.8258347691566634 >500 0.003190163427462129 1.8278208112807102 >1k 0.0017944669279474476 2.528728194092536 >5k 3.987704284327661E-4 1.8776373690673354 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 6223 1.0058300320191111 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 5122 0.8278742445768742 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 2328 0.3762770873438038 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 2224 0.3594674580122937 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 2007 0.3243935198878927 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 1695 0.2739646318933623 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 1677 0.27105527297060095 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 1476 0.23856743166643232 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 1470 0.23759764535884517 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 1343 0.21707050184825108 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 1059 0.17116728328912725 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 963 0.15565070236773326 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 856 0.13835617988242957 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 812 0.13124441362679068 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 766 0.12380938526862272 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 750 0.1212232884483904 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 737 0.11912208478195165 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 691 0.11168705642378368 No Hit CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC 687 0.11104053221872562 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 668 0.10796954224469972 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 666 0.10764628014217067 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 626 0.10118103809158986 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 8.081552563226027E-5 0.0 16-17 0.0 0.0 0.0 4.040776281613013E-4 0.0 18-19 0.0 0.0 0.0 4.8489315379356157E-4 0.0 20-21 0.0 0.0 0.0 6.465242050580822E-4 0.0 22-23 0.0 0.0 0.0 8.889707819548628E-4 0.0 24-25 0.0 0.0 0.0 0.0019395726151742463 0.0 26-27 0.0 0.0 0.0 0.00307098997402589 0.0 28-29 0.0 0.0 0.0 0.006465242050580821 0.0 30-31 0.0 0.0 0.0 0.015920658549555274 0.0 32-33 0.0 0.0 0.0 0.04000368518796883 0.0 34-35 0.0 0.0 0.0 0.07669393382501499 0.0 36-37 0.0 0.0 0.0 0.1259105889350615 0.0 38-39 0.0 0.0 0.0 0.2082616095543347 0.0 40-41 0.0 0.0 0.0 0.3312628395666348 0.0 42-43 0.0 0.0 0.0 0.4187860538263727 0.0 44-45 0.0 0.0 0.0 0.515764684585085 0.0 46-47 0.0 0.0 0.0 0.6434532150840562 0.0 48-49 0.0 0.0 0.0 0.7595851254176142 0.0 50-51 0.0 0.0 0.0 0.8848491901476176 0.0 52-53 0.0 0.0 0.0 1.0376713491182217 0.0 54-55 0.0 0.0 0.0 1.1891196441530776 0.0 56-57 0.0 0.0 0.0 1.3467907346616173 0.0 58-59 0.0 0.0 0.0 1.5309693175775385 0.0 60-61 0.0 0.0 0.0 1.7132083278782853 0.0 62-63 0.0 0.0 0.0 1.9057917254599617 0.0 64-65 1.6163105126452054E-4 0.0 0.0 2.1492080886643294 0.0 66-67 1.6163105126452054E-4 0.0 0.0 2.409353265674575 0.0 68-69 1.6163105126452054E-4 0.0 0.0 2.715805738872106 0.0 70-71 1.6163105126452054E-4 0.0 0.0 3.1670796340026475 0.0 72-73 1.6163105126452054E-4 0.0 0.0 3.554186001781174 0.0 74-75 1.6163105126452054E-4 0.0 0.0 3.9100167611400165 0.0 76-77 1.6163105126452054E-4 0.0 0.0 4.319104951890518 0.0 78-79 1.6163105126452054E-4 0.0 0.0 4.748720286151613 0.0 80-81 1.6163105126452054E-4 0.0 0.0 5.230784896548046 0.0 82-83 1.6163105126452054E-4 0.0 0.0 5.834557688546662 0.0 84-85 1.6163105126452054E-4 0.0 0.0 6.406489163446167 8.081552563226027E-5 86-87 1.6163105126452054E-4 0.0 0.0 6.9804410264864805 1.6163105126452054E-4 88 1.6163105126452054E-4 0.0 0.0 7.472850024163842 1.6163105126452054E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCTCGC 85 9.094947E-12 66.47048 1 TCTCGCT 120 1.2927558E-8 41.841602 2 CTCGCTC 115 9.699634E-7 34.50883 3 GTACAAA 245 9.094947E-12 30.748255 1 TAGGACC 75 0.004950416 30.236307 4 TCGCTCT 120 5.1320538E-5 28.346539 4 TGTACTG 115 8.747735E-5 26.21179 5 GTATAAC 120 9.409528E-4 26.157366 1 GTACAAG 325 3.092282E-10 23.179453 1 ATGGGTC 185 4.0246396E-6 21.725086 5 CGCTCTG 140 3.3195366E-4 21.531115 5 CATGGGT 720 0.0 21.259903 4 CATATAC 280 1.1078373E-7 20.247528 3 CATAGAC 140 0.0041715614 20.247528 3 ACGTCAT 100 9.942916E-6 19.572514 26-27 CTGTTGC 340 4.0563464E-10 19.256008 9 TTAGGAC 180 7.996374E-4 18.897692 3 ATACTAC 150 0.0062165116 18.897692 3 GTACATA 335 2.5014924E-7 18.739607 1 GTCATCG 105 1.620987E-5 18.494024 28-29 >>END_MODULE