##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295131_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 618693 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.236597149151518 33.0 33.0 33.0 27.0 33.0 2 29.702962535538628 33.0 33.0 33.0 22.0 33.0 3 30.624613499748662 33.0 33.0 33.0 27.0 33.0 4 34.890132909213456 37.0 37.0 37.0 33.0 37.0 5 35.27348297136059 37.0 37.0 37.0 33.0 37.0 6 35.05103985336831 37.0 37.0 37.0 33.0 37.0 7 34.9271302568479 37.0 37.0 37.0 33.0 37.0 8 34.95121489979683 37.0 37.0 37.0 33.0 37.0 9 34.83326140751552 37.0 37.0 37.0 33.0 37.0 10-11 34.828793925258566 37.0 37.0 37.0 30.0 37.0 12-13 34.7147753409203 37.0 37.0 37.0 27.0 37.0 14-15 35.984138336784156 37.0 37.0 40.0 27.0 40.0 16-17 35.96446298891372 37.0 37.0 40.0 27.0 40.0 18-19 36.07236383796164 37.0 37.0 40.0 27.0 40.0 20-21 36.11684874404592 37.0 37.0 40.0 27.0 40.0 22-23 36.12830838558057 37.0 37.0 40.0 27.0 40.0 24-25 36.093046955436705 37.0 37.0 40.0 27.0 40.0 26-27 35.97084014204137 37.0 37.0 40.0 27.0 40.0 28-29 35.85712704038998 37.0 37.0 40.0 27.0 40.0 30-31 35.60477005558492 37.0 37.0 40.0 27.0 40.0 32-33 35.46707575485742 37.0 37.0 40.0 27.0 40.0 34-35 35.39064932042224 37.0 35.0 40.0 27.0 40.0 36-37 35.32912203629263 37.0 35.0 40.0 27.0 40.0 38-39 35.18693924127152 37.0 33.0 40.0 27.0 40.0 40-41 35.02605977439538 37.0 33.0 40.0 24.5 40.0 42-43 34.79045099912234 37.0 33.0 40.0 22.0 40.0 44-45 34.48518570599635 37.0 33.0 40.0 22.0 40.0 46-47 34.10289755985602 37.0 33.0 40.0 22.0 40.0 48-49 33.7926701934562 37.0 33.0 40.0 15.0 40.0 50-51 33.6375779263706 37.0 33.0 40.0 15.0 40.0 52-53 33.61175979039685 37.0 33.0 40.0 15.0 40.0 54-55 33.47969671549541 37.0 33.0 40.0 15.0 40.0 56-57 33.263376989880285 37.0 33.0 40.0 15.0 40.0 58-59 33.14113138503264 37.0 33.0 40.0 15.0 40.0 60-61 32.916890929750295 37.0 33.0 40.0 15.0 40.0 62-63 32.67593297483566 37.0 33.0 40.0 15.0 40.0 64-65 32.56517368064614 37.0 33.0 37.0 15.0 40.0 66-67 32.304227621776874 37.0 33.0 37.0 15.0 40.0 68-69 32.07684182623692 37.0 33.0 37.0 15.0 40.0 70-71 31.77225780799201 37.0 27.0 37.0 15.0 40.0 72-73 31.481267769313696 37.0 27.0 37.0 15.0 38.5 74-75 31.2499010009811 35.0 27.0 37.0 15.0 37.0 76-77 30.93156137211832 33.0 27.0 37.0 15.0 37.0 78-79 30.689252181615114 33.0 27.0 37.0 15.0 37.0 80-81 30.59384056389841 33.0 27.0 37.0 15.0 37.0 82-83 30.499279933666617 33.0 27.0 37.0 15.0 37.0 84-85 30.289932971603044 33.0 27.0 37.0 15.0 37.0 86-87 30.263120804664027 33.0 27.0 37.0 15.0 37.0 88-89 30.300114919677448 33.0 27.0 37.0 15.0 37.0 90-91 30.302977405595342 33.0 27.0 37.0 15.0 37.0 92-93 30.221508890515977 33.0 27.0 37.0 15.0 37.0 94-95 30.32533421260625 33.0 27.0 37.0 15.0 37.0 96-97 30.29009137003328 33.0 27.0 37.0 15.0 37.0 98-99 30.189964166395935 33.0 27.0 37.0 15.0 37.0 100 29.59607753764791 33.0 27.0 37.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7276227945621905 1101 2 4.341881867215072 1101 3 2.440963882821414 1101 4 1.4459817547001137 1101 5 0.5928808006859825 1101 6 0.4713648682703564 1101 7 0.475923752696886 1101 8 0.6552554816136933 1101 9 0.8605039056072883 1101 10-11 0.7523320356166181 1101 12-13 0.660910073211852 1101 14-15 1.0994699773081962 1101 16-17 0.992846026888003 1101 18-19 1.1305307410813157 1101 20-21 1.1837147449602554 1101 22-23 1.1597053241209636 1101 24-25 0.9793060565227734 1101 26-27 1.117371254021478 1101 28-29 0.8568901961410305 1101 30-31 0.8241666557090142 1101 32-33 1.0992178167434972 1101 34-35 1.288668313118535 1101 36-37 1.1271713820800429 1101 38-39 1.100980546421603 1101 40-41 1.1023631351284706 1101 42-43 1.1654705268642616 1101 44-45 1.2284482135767476 1101 46-47 1.2136763064304574 1101 48-49 1.1585979272366416 1101 50-51 1.0055571559753318 1101 52-53 0.9971430352004518 1101 54-55 0.9946777595213305 1101 56-57 0.8995341337112919 1101 58-59 0.9488628284081955 1101 60-61 0.8141134862442101 1101 62-63 0.9244440817803365 1101 64-65 0.9279428672535417 1101 66-67 0.8679662323487207 1101 68-69 0.7422930259868181 1101 70-71 0.8021140194368499 1101 72-73 0.8618871685321707 1101 74-75 0.797121806377632 1101 76-77 0.8099283136411692 1101 78-79 0.9530246471010599 1101 80-81 1.0037223684013235 1101 82-83 0.9449927019866635 1101 84-85 0.9848922642824647 1101 86-87 1.1050870655579885 1101 88-89 0.8852922303884441 1101 90-91 0.8291960637429199 1101 92-93 0.9719405280910038 1101 94-95 1.1009592932038288 1101 96-97 0.9349504528895451 1101 98-99 0.7810877410058765 1101 100 0.8306390607831879 1102 1 0.5310284311934161 1102 2 4.088078722915661 1102 3 2.36482763901671 1102 4 1.1312285808283278 1102 5 0.49745030492469056 1102 6 0.4931670405786974 1102 7 0.571943403421983 1102 8 0.5396080301100881 1102 9 0.5734146520689194 1102 10-11 0.5952483068387977 1102 12-13 0.6847164039914162 1102 14-15 0.8464623068315049 1102 16-17 0.7761149428887109 1102 18-19 0.7844979088735684 1102 20-21 0.7415338858485327 1102 22-23 0.7686583017605813 1102 24-25 0.8670061285051318 1102 26-27 0.942829511131464 1102 28-29 0.7778213589999652 1102 30-31 0.9194246459799302 1102 32-33 1.0653282248422045 1102 34-35 0.9586498361185107 1102 36-37 0.8374750605160486 1102 38-39 0.8782857011836782 1102 40-41 1.0174996035914887 1102 42-43 1.0620921701710486 1102 44-45 1.1386702814418967 1102 46-47 1.051537765609126 1102 48-49 1.1398469816085424 1102 50-51 0.8424941920444553 1102 52-53 0.7905387685264529 1102 54-55 0.7951169894476493 1102 56-57 0.8535680112679174 1102 58-59 0.8377945093567121 1102 60-61 0.9284502398857342 1102 62-63 0.9960252636664606 1102 64-65 0.8888259578183622 1102 66-67 0.9282245206357729 1102 68-69 0.8700198735721969 1102 70-71 0.8840587122280752 1102 72-73 0.9470785178116543 1102 74-75 0.9424499450605843 1102 76-77 0.9280705040138741 1102 78-79 0.810479520581751 1102 80-81 0.7697464664440474 1102 82-83 0.777081817979127 1102 84-85 0.6565186070743714 1102 86-87 1.0884073320302576 1102 88-89 1.1005922638579477 1102 90-91 0.9660897728516673 1102 92-93 0.9224790203127817 1102 94-95 0.9063719538459836 1102 96-97 0.9119378766088779 1102 98-99 1.099862413302759 1102 100 1.0540663379702835 1103 1 0.6602799415984286 1103 2 4.09582654121953 1103 3 2.375388819743197 1103 4 1.2173426443772257 1103 5 0.647521163431442 1103 6 0.6856993930811157 1103 7 0.605293519033232 1103 8 0.6559628261121802 1103 9 0.8249949832392076 1103 10-11 0.769415719384483 1103 12-13 0.9299078151945182 1103 14-15 0.9956194635406916 1103 16-17 1.0714795378764066 1103 18-19 1.1522990109098146 1103 20-21 1.1809533588850059 1103 22-23 0.9716551038042951 1103 24-25 0.9264934765322792 1103 26-27 1.006688409468147 1103 28-29 1.1724892563670082 1103 30-31 1.3440465785524722 1103 32-33 1.2517094063145677 1103 34-35 1.3034545556679973 1103 36-37 1.1327324353948498 1103 38-39 1.097894627490824 1103 40-41 0.9758084627695283 1103 42-43 1.1503786063610946 1103 44-45 0.977203790056798 1103 46-47 0.9344872043351629 1103 48-49 0.9143560999795426 1103 50-51 0.7970293695636599 1103 52-53 0.8886696423237197 1103 54-55 0.86535198795589 1103 56-57 0.8922721397082896 1103 58-59 0.771385637125789 1103 60-61 0.8811032383193904 1103 62-63 1.0446018001772401 1103 64-65 0.8952673224040169 1103 66-67 0.9490811626884295 1103 68-69 0.9298067386426894 1103 70-71 0.9691238834073133 1103 72-73 1.106096428231588 1103 74-75 1.2187025556371474 1103 76-77 0.9431559071614828 1103 78-79 1.1026822877929057 1103 80-81 1.183544995197682 1103 82-83 1.1371595758667965 1103 84-85 1.0720967565485253 1103 86-87 0.8584003860318958 1103 88-89 0.9618774134066896 1103 90-91 0.9919321499681004 1103 92-93 0.9761823096392526 1103 94-95 0.7284087163581034 1103 96-97 0.9992325267916158 1103 98-99 0.8410097617028462 1103 100 1.0481972024440651 1104 1 0.5387749996614914 1104 2 3.7168053453835377 1104 3 2.1204973826354525 1104 4 1.0126198532163357 1104 5 0.4506610784977738 1104 6 0.4226692622982995 1104 7 0.39868579232422263 1104 8 0.5337191773467254 1104 9 0.4372948629791438 1104 10-11 0.43267580056002686 1104 12-13 0.3561510505660124 1104 14-15 0.7584534670733305 1104 16-17 0.6231520218104123 1104 18-19 0.6320421194445274 1104 20-21 0.7097188846469606 1104 22-23 0.686355134808835 1104 24-25 0.5514294709326677 1104 26-27 0.6587715862128576 1104 28-29 0.5946467030955631 1104 30-31 0.5638699935624345 1104 32-33 0.6731246229705903 1104 34-35 0.9715855216945286 1104 36-37 0.7839741440272618 1104 38-39 0.76190104919025 1104 40-41 0.8653400827101478 1104 42-43 0.9387643499065135 1104 44-45 0.7753113382547738 1104 46-47 0.974053821833003 1104 48-49 0.7192846485909143 1104 50-51 0.8046472103318223 1104 52-53 0.8497689179447576 1104 54-55 0.9595077482249721 1104 56-57 1.1450167467961165 1104 58-59 0.7498292558262278 1104 60-61 0.960844820115021 1104 62-63 0.9774728447486183 1104 64-65 0.8966243585571974 1104 66-67 0.7447424051252014 1104 68-69 0.768439813917972 1104 70-71 0.6057353212666854 1104 72-73 0.7719408088922677 1104 74-75 0.7476436563925297 1104 76-77 0.696383198679392 1104 78-79 0.9124247621436865 1104 80-81 0.8879982581914057 1104 82-83 0.9632874837175258 1104 84-85 1.009294931977891 1104 86-87 1.0993262710707938 1104 88-89 1.0352877257070396 1104 90-91 0.6738994824495919 1104 92-93 0.6403936666988947 1104 94-95 0.7287675000403162 1104 96-97 0.6372256635122753 1104 98-99 0.6794361399959072 1104 100 0.6390052936913868 1105 1 0.4937227890261511 1105 2 3.7772531039780013 1105 3 2.1305151132348428 1105 4 0.9687665190624628 1105 5 0.465793628371614 1105 6 0.3608095581334112 1105 7 0.3210676841222835 1105 8 0.32756815722765964 1105 9 0.32367736785883494 1105 10-11 0.3354791395326231 1105 12-13 0.3845339432662769 1105 14-15 0.5876434678048454 1105 16-17 0.662390116937928 1105 18-19 0.38576879650725715 1105 20-21 0.4685211925277315 1105 22-23 0.300554360793285 1105 24-25 0.5519911721413067 1105 26-27 0.641091496387105 1105 28-29 0.4341479124222971 1105 30-31 0.43251348078931073 1105 32-33 0.46201437626328357 1105 34-35 0.6387161603328337 1105 36-37 0.5328736741141284 1105 38-39 0.365458141836406 1105 40-41 0.33913171391368735 1105 42-43 0.5916332932580062 1105 44-45 0.49105598278935503 1105 46-47 0.31230709653653577 1105 48-49 0.32484383593264 1105 50-51 0.25322382753781625 1105 52-53 0.21512550967535304 1105 54-55 0.30214751748334123 1105 56-57 0.46798333036390005 1105 58-59 0.20564673863845684 1105 60-61 0.19639182710391623 1105 62-63 0.4565406936100658 1105 64-65 0.2720343859252594 1105 66-67 0.42472308376476064 1105 68-69 0.5210875021473811 1105 70-71 0.3565539245784848 1105 72-73 0.11653942040171472 1105 74-75 0.4576958147765957 1105 76-77 0.13164038526172916 1105 78-79 0.15458852228479003 1105 80-81 0.20393443267815314 1105 82-83 0.453517075820848 1105 84-85 0.2506278968429747 1105 86-87 0.21140786898445896 1105 88-89 0.16250187349454848 1105 90-91 0.14058087623493876 1105 92-93 0.09970501811426757 1105 94-95 0.23233887470169634 1105 96-97 0.12229602275721163 1105 98-99 0.2561964564296275 1105 100 0.11203434656155054 1106 1 0.30135779511232386 1106 2 3.7892964104993965 1106 3 1.9643656363553212 1106 4 0.7875013210844486 1106 5 0.2516407461395005 1106 6 0.11412706760063429 1106 7 -0.03351074428738343 1106 8 0.3040552477795373 1106 9 0.2327936688057477 1106 10-11 0.03989498134907876 1106 12-13 0.04587956029929785 1106 14-15 0.09172949988197132 1106 16-17 0.15171766593874736 1106 18-19 0.05465300167081466 1106 20-21 0.08924557850568249 1106 22-23 -0.034787959259588774 1106 24-25 0.11106532514642709 1106 26-27 0.10157633457978932 1106 28-29 -0.005263234186060117 1106 30-31 -0.08710556214795417 1106 32-33 0.25923417624350975 1106 34-35 0.3410137119535932 1106 36-37 0.19356933580888125 1106 38-39 0.3352359168831427 1106 40-41 0.14960674831075949 1106 42-43 0.4666703717157432 1106 44-45 0.1581095874857894 1106 46-47 0.17118495018122815 1106 48-49 -0.024460932510649513 1106 50-51 0.05910951248795726 1106 52-53 -0.04319808037963213 1106 54-55 0.02632177813470804 1106 56-57 -0.12725621462825387 1106 58-59 -0.0851606528089448 1106 60-61 0.04939354821470232 1106 62-63 0.13418854238917532 1106 64-65 0.13771288958115235 1106 66-67 0.07841364022373654 1106 68-69 0.02282844397093342 1106 70-71 -0.08697062652777987 1106 72-73 -0.0513663603742458 1106 74-75 -0.06057443816132846 1106 76-77 0.04833996400575913 1106 78-79 0.10693453426274147 1106 80-81 0.005393326843528001 1106 82-83 0.14528099371922565 1106 84-85 0.2070708340042735 1106 86-87 0.2568592150651483 1106 88-89 0.07904866944097222 1106 90-91 -0.008314758310842052 1106 92-93 0.0846004694324165 1106 94-95 -0.012363863601759562 1106 96-97 0.2053503715365963 1106 98-99 0.2428012874943093 1106 100 0.1945940365940224 1107 1 0.3735950606546652 1107 2 3.5247637200571944 1107 3 2.1471441754644083 1107 4 1.0561666474169584 1107 5 0.36535064884795787 1107 6 0.23279943288715543 1107 7 0.14505432094392035 1107 8 0.33677043630252257 1107 9 0.16594725218325834 1107 10-11 0.2885542805772161 1107 12-13 0.41499186021152923 1107 14-15 0.23217587954994912 1107 16-17 0.18129557066210822 1107 18-19 0.14877866459730882 1107 20-21 0.24426644240530493 1107 22-23 0.11718800711180677 1107 24-25 0.08731325470409246 1107 26-27 0.07883006467550047 1107 28-29 0.024885760673306834 1107 30-31 0.025908144015303947 1107 32-33 0.038860198816557556 1107 34-35 0.3172091243153119 1107 36-37 0.0784073738764306 1107 38-39 -0.14376985285947086 1107 40-41 0.05237514692672107 1107 42-43 0.4614378992611492 1107 44-45 0.3338183771596448 1107 46-47 0.19975485469342402 1107 48-49 0.3253511604123389 1107 50-51 0.16000274475364762 1107 52-53 0.14734667966902748 1107 54-55 0.18865050608572886 1107 56-57 0.24399691715635896 1107 58-59 0.2919603820052856 1107 60-61 0.20204618903228067 1107 62-63 0.3026455596764066 1107 64-65 0.392172176931048 1107 66-67 0.3118589002580947 1107 68-69 0.3402748214392446 1107 70-71 0.2754200222218124 1107 72-73 0.2344041741243288 1107 74-75 0.2153982023042964 1107 76-77 0.1642874842042552 1107 78-79 0.24052425782981857 1107 80-81 0.6039725029779532 1107 82-83 0.32361014400749255 1107 84-85 0.20724955744597295 1107 86-87 0.27335456385631574 1107 88-89 0.010133185281915758 1107 90-91 0.11264169256055823 1107 92-93 0.13062972948437235 1107 94-95 0.10564667503219738 1107 96-97 -0.029600839713413762 1107 98-99 0.09196420188523291 1107 100 -0.04385877931753157 1108 1 0.5343452467851044 1108 2 3.7553573420589927 1108 3 2.082871779198605 1108 4 0.9990203113436991 1108 5 0.44285386187772957 1108 6 0.1661937011424186 1108 7 0.1299064142945099 1108 8 0.23724214554854228 1108 9 0.13930485220557642 1108 10-11 0.04708731524360843 1108 12-13 0.10758966183402663 1108 14-15 0.11165829746482103 1108 16-17 0.1747729593003129 1108 18-19 0.18482842954727374 1108 20-21 0.08930614665637648 1108 22-23 0.10775543138836952 1108 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2210 70-71 0.7423632046440432 2210 72-73 0.6272134095212643 2210 74-75 0.057916330085220125 2210 76-77 0.6035590272622322 2210 78-79 0.6291462806994303 2210 80-81 0.7871549408400789 2210 82-83 0.85031559375469 2210 84-85 0.8549819823317613 2210 86-87 0.8994219845052598 2210 88-89 0.9239074064674213 2210 90-91 0.9044547989696134 2210 92-93 0.9070209330939818 2210 94-95 0.7118536616149846 2210 96-97 0.7393934632073567 2210 98-99 0.8240904756764778 2210 100 0.6700857757818532 2211 1 0.5039552165864727 2211 2 4.107654443111997 2211 3 2.160127410301371 2211 4 0.8703389038566272 2211 5 0.5295154245505245 2211 6 0.3440587734186167 2211 7 0.31400261242669103 2211 8 0.262804648206874 2211 9 0.37957840197255166 2211 10-11 0.270829089610622 2211 12-13 0.2577093626427711 2211 14-15 0.1838752536616184 2211 16-17 0.19246680473437294 2211 18-19 0.4120548949707583 2211 20-21 0.2702807056773793 2211 22-23 0.2073516690157433 2211 24-25 0.22549493687251498 2211 26-27 0.21382827761053136 2211 28-29 0.28106256373874317 2211 30-31 0.03890592472996701 2211 32-33 0.3940840390945084 2211 34-35 0.8224549912080619 2211 36-37 0.37587161179711615 2211 38-39 0.5006998044396553 2211 40-41 0.6582816044652304 2211 42-43 0.6110796550732687 2211 44-45 0.43711488864257575 2211 46-47 0.5476091299346706 2211 48-49 0.44774537032182593 2211 50-51 0.31483633991278026 2211 52-53 0.5541460462999481 2211 54-55 0.5363281528120751 2211 56-57 0.3815053329855971 2211 58-59 0.6124071066892185 2211 60-61 0.5488438101578836 2211 62-63 0.5688351215545708 2211 64-65 0.3429800965380636 2211 66-67 0.39210227357180827 2211 68-69 0.5308819248147145 2211 70-71 0.34951048926916783 2211 72-73 0.575191230786757 2211 74-75 0.6439924886835371 2211 76-77 0.4121471622000854 2211 78-79 0.2107602750853843 2211 80-81 0.4105798640232621 2211 82-83 0.18685811381856254 2211 84-85 0.6258155001435242 2211 86-87 0.5540422098345914 2211 88-89 0.5646563298513172 2211 90-91 0.4879076743241839 2211 92-93 0.546261560851935 2211 94-95 0.5550883211268385 2211 96-97 0.4798072261835671 2211 98-99 0.37581453727160863 2211 100 0.41190305063700094 2212 1 0.6483809868945976 2212 2 4.2936488408711 2212 3 2.3074663458756 2212 4 1.2609271391507448 2212 5 0.46497760942447286 2212 6 0.766635804230944 2212 7 0.823638466768422 2212 8 0.541123975937964 2212 9 0.34462041877927874 2212 10-11 0.10715962182351291 2212 12-13 0.045356421466294705 2212 14-15 0.11143827887169522 2212 16-17 0.2807020988520179 2212 18-19 0.4646879481920436 2212 20-21 0.31254961324040664 2212 22-23 0.3973236578112562 2212 24-25 0.45599913855318874 2212 26-27 0.3435481655657071 2212 28-29 0.3553202668199802 2212 30-31 0.5061328154862679 2212 32-33 0.7019271763494075 2212 34-35 0.7910264197794987 2212 36-37 0.6833225921892776 2212 38-39 0.5164421013584146 2212 40-41 0.6349482711319041 2212 42-43 0.6229003833645876 2212 44-45 0.6126611071299735 2212 46-47 0.7389536677497972 2212 48-49 0.26169495015376043 2212 50-51 -0.1249675816558522 2212 52-53 -0.11039176882610491 2212 54-55 -0.02708921413469767 2212 56-57 0.08923642542257681 2212 58-59 -0.09145843952926214 2212 60-61 0.06077658326712054 2212 62-63 0.43270066777306226 2212 64-65 0.10230782763050428 2212 66-67 0.08277454247937044 2212 68-69 0.5822544738343254 2212 70-71 0.24900628758849308 2212 72-73 0.06709599269152022 2212 74-75 -0.023122357254838022 2212 76-77 0.24393987928691985 2212 78-79 0.22370145155597143 2212 80-81 0.5592353262081353 2212 82-83 0.17615783370651528 2212 84-85 0.3375802060258799 2212 86-87 0.22827190171133793 2212 88-89 0.021407030131427973 2212 90-91 0.0738180384698417 2212 92-93 0.08144363368106511 2212 94-95 0.6351443435358028 2212 96-97 0.5338688508334286 2212 98-99 0.3311926885321128 2212 100 0.1375613139423244 2213 1 -0.899930701417091 2213 2 -25.620636873414615 2213 3 -14.617468719059465 2213 4 -7.113358575134971 2213 5 -2.812944468497605 2213 6 -1.9816758840807438 2213 7 -1.4974004942705363 2213 8 -1.6905643357503521 2213 9 -1.7309639968051371 2213 10-11 -1.4946585599946758 2213 12-13 -1.7720461759363033 2213 14-15 -1.8208993834659637 2213 16-17 -1.7165706284207118 2213 18-19 -1.4612860777819847 2213 20-21 -1.837969867279078 2213 22-23 -2.343845173357572 2213 24-25 -2.0075073549533116 2213 26-27 -2.0737245617070172 2213 28-29 -2.260653759154046 2213 30-31 -2.1313996520461984 2213 32-33 -2.099371524949291 2213 34-35 -2.264038515285435 2213 36-37 -2.090833251966565 2213 38-39 -2.1368046518883332 2213 40-41 -2.154921858738234 2213 42-43 -2.5054113049471027 2213 44-45 -2.642793438324574 2213 46-47 -2.733773604977472 2213 48-49 -2.3229803745215634 2213 50-51 -2.1531493998376696 2213 52-53 -2.3075346259689553 2213 54-55 -2.1955307725762587 2213 56-57 -2.468296042109891 2213 58-59 -2.2670428551136794 2213 60-61 -2.019093546603006 2213 62-63 -2.365870760798373 2213 64-65 -2.1624973671746943 2213 66-67 -2.2239787042738755 2213 68-69 -2.3503429287630766 2213 70-71 -2.223201504619297 2213 72-73 -2.0718650462695187 2213 74-75 -2.2898756040080848 2213 76-77 -2.116189990842951 2213 78-79 -2.0211037432492205 2213 80-81 -2.4993361023632907 2213 82-83 -2.3124135948649105 2213 84-85 -2.1693029108572404 2213 86-87 -1.9425073190678788 2213 88-89 -2.172878684154284 2213 90-91 -2.2103378056860024 2213 92-93 -2.2882966260591964 2213 94-95 -2.3269335785421177 2213 96-97 -2.4067804998159232 2213 98-99 -1.854261856922431 2213 100 -1.765555569174559 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 7.0 4 10.0 5 21.0 6 11.0 7 6.0 8 13.0 9 28.0 10 121.0 11 527.0 12 1521.0 13 2888.0 14 3926.0 15 4685.0 16 5484.0 17 5613.0 18 5897.0 19 6637.0 20 8065.0 21 10164.0 22 11837.0 23 11440.0 24 9518.0 25 8230.0 26 8073.0 27 8716.0 28 10049.0 29 11753.0 30 14054.0 31 17136.0 32 21755.0 33 28500.0 34 38795.0 35 55067.0 36 83288.0 37 128832.0 38 95287.0 39 736.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.86604885169834 21.149409556580903 10.431818774275957 27.552722817444803 2 12.358496885256882 23.75053887546352 34.17646947689148 29.71449476238812 3 17.51062320084436 30.01343154036008 28.517051267753153 23.95889399104241 4 11.32869958781217 21.06667744281904 33.27632748727067 34.32829548209811 5 10.44369429535906 38.94293740032867 34.493689843035355 16.119678461276926 6 30.77815064893058 35.177131767274496 17.74277281364978 16.30194477014515 7 22.114893314274997 35.22423728923182 23.531009682238878 19.129859714254298 8 26.70875248011462 33.425396050250725 21.33472613228248 18.531125337352183 9 26.470897374740264 14.852162382865874 22.868623820573564 35.808316421820294 10-11 21.906322741932748 28.20893241625102 26.730141224963027 23.154603616853205 12-13 26.03904992371609 26.56572018560397 27.843910263925352 19.55131962675459 14-15 25.616147503472675 25.27033517195209 26.81909252763992 22.29442479693531 16-17 19.49850229809321 29.183560447734042 30.977649679237125 20.340287574935626 18-19 21.247709121717488 26.877833029230274 33.64323011614662 18.231227732905623 20-21 21.198888319431937 26.260462424194415 33.0192248307662 19.521424425607456 22-23 19.235097158876872 26.604588673339553 31.123094856618106 23.03721931116547 24-25 20.207712087249423 27.523225745434676 30.1343386977739 22.134723469542 26-27 21.553579020709968 25.08903297978794 31.51217848303645 21.845209516465644 28-29 20.1751663150407 24.29541431238936 34.1124879760086 21.41693139656134 30-31 21.976263113732376 26.64506710253811 31.512325170444168 19.86634461328535 32-33 17.993615387723764 26.660766961651916 32.78651957813069 22.559098072493637 34-35 17.42000609385014 27.10146907047772 33.9500999342931 21.528424901379037 36-37 22.26519185198835 26.3235184772102 31.160470957918317 20.25081871288313 38-39 22.077306985108404 25.427653512759896 31.228627427208988 21.266412074922712 40-41 19.894186907367477 26.45901157057924 31.515199518943227 22.131602003110057 42-43 22.242499676710203 25.63308224492435 31.229794387689125 20.894623690676323 44-45 23.825542411731806 24.504870471011564 29.819551716222726 21.850035401033903 46-47 21.212576810596104 26.451459583197938 32.56066036500492 19.775303241201033 48-49 22.901452035614565 26.66023724719528 30.474720740617382 19.963589976572766 50-51 21.078001434037123 27.271683748737942 29.63541990854174 22.0148949086832 52-53 19.833545544574466 27.576951838153562 27.430465449988883 25.15903716728309 54-55 21.221180033192326 28.33602660559581 26.303825362783368 24.138967998428498 56-57 22.69146456204108 25.593057580822993 27.71414576105688 24.001332096079047 58-59 20.974443037995147 24.42614443749303 30.728088085757843 23.871324438753984 60-61 22.425198148725915 26.46625207524721 28.535356392892997 22.573193383133876 62-63 18.79913120866916 26.9697143610043 29.41587767081797 24.81527675950857 64-65 18.12037787734587 27.065986269734328 30.411819054077977 24.40181679884183 66-67 22.178122741697937 26.20519569122029 28.49352761534619 23.12315395173559 68-69 22.64952635637984 25.50302908797532 28.18660540045885 23.66083915518599 70-71 20.64602393022547 26.26172885814458 28.453379228835914 24.63886798279404 72-73 22.571132477216103 25.865400005496912 28.721127061140223 22.84234045614676 74-75 23.857726041792976 24.53085970655996 27.68562305484823 23.925791196798833 76-77 21.13751806597841 26.07983626646146 30.691955264272526 22.090690403287606 78-79 22.779371017126156 26.25196819843967 29.88080881235592 21.087851972078255 80-81 20.796842504302113 26.48161185531971 29.540577263024144 23.180968377354038 82-83 19.380379408700488 26.67426960470919 27.808082661340837 26.137268325249487 84-85 20.64436970633094 26.711897504786986 26.889959578373578 25.75377321050849 86-87 21.89775240213638 25.181372612412222 27.89242437958281 25.028450605868585 88-89 20.616559426406724 24.223905700774214 30.182509155362723 24.977025717456332 90-91 21.62021702900132 25.544552694919552 28.634217387860538 24.201012888218585 92-93 18.67031546891177 26.244058640540985 29.143191511200587 25.942434379346658 94-95 17.843094944263814 26.277511482227702 30.071758366893036 25.807635206615444 96-97 21.26836269829532 25.466982500632398 28.52793732457597 24.736717476496313 98-99 21.265200599012907 24.852004535429607 28.25155632636667 25.631238539190814 100 19.54141087915395 25.386148802801177 29.835152566396882 25.23728775164799 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 68.0 1 47.5 2 28.0 3 26.5 4 27.0 5 88.0 6 299.5 7 607.5 8 897.0 9 1010.0 10 721.0 11 384.5 12 307.0 13 325.0 14 391.0 15 468.5 16 522.5 17 590.0 18 677.5 19 785.0 20 939.5 21 1151.0 22 1410.0 23 1764.0 24 2235.5 25 2838.0 26 3699.5 27 4856.0 28 6207.0 29 7652.5 30 9315.5 31 10925.5 32 12199.0 33 13441.0 34 14333.5 35 15025.0 36 15852.5 37 16416.5 38 17018.5 39 17423.5 40 17482.5 41 17333.0 42 16802.0 43 16384.5 44 16063.0 45 15790.5 46 16645.0 47 19633.0 48 22283.0 49 29487.5 50 58713.0 51 59805.5 52 27816.5 53 15288.0 54 12311.5 55 11045.5 56 10434.5 57 9554.5 58 8293.0 59 7278.0 60 6238.5 61 5051.0 62 4123.0 63 3244.5 64 2452.5 65 1780.5 66 1235.0 67 833.5 68 560.0 69 419.5 70 344.0 71 287.0 72 224.5 73 145.0 74 89.5 75 68.5 76 58.5 77 47.0 78 36.5 79 25.0 80 13.5 81 12.5 82 9.0 83 5.0 84 1.5 85 1.0 86 0.5 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.7947398790676475 2 5.893391391206947 3 0.0 4 0.006950135204374383 5 0.1677730312125723 6 0.05948022686534355 7 0.03895308335474945 8 0.044286908046478626 9 0.04493343225153671 10-11 0.04566077198222705 12-13 0.045175878828433484 14-15 0.04751952907176903 16-17 0.03968042308543979 18-19 0.03353844313738801 20-21 0.02747727871496849 22-23 0.02747727871496849 24-25 0.023355686907723217 26-27 0.016486367228981093 28-29 0.022305085074503832 30-31 0.026184230304852327 32-33 0.00290935892276137 34-35 0.005899533371154999 36-37 0.006303610999316301 38-39 0.008970523345180888 40-41 0.008889707819548629 42-43 0.008566445717019588 44-45 0.010829280434722876 46-47 0.020365512459329587 48-49 0.029416851330142737 50-51 0.025780152676691023 52-53 0.03321518103485897 54-55 0.028527880548187872 56-57 0.01923409510047794 58-59 0.018425939844155338 60-61 0.014870056716335888 62-63 0.02141611429254897 64-65 0.02141611429254897 66-67 0.022951609279561913 68-69 0.0307098997402589 70-71 0.030144191060833075 72-73 0.02650749240738137 74-75 0.02715401661243945 76-77 0.02052714351059411 78-79 0.017294522485303696 80-81 0.016405551703348835 82-83 0.011718251216677739 84-85 0.014304348036910068 86-87 0.012445590947368081 88-89 0.010748464909090615 90-91 0.010667649383458355 92-93 0.006869319678742123 94-95 0.0031518054996581505 96-97 0.0021820191920710274 98-99 0.0013738639357484246 100 0.1078079111934352 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 618693.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.767871326139655 #Duplication Level Percentage of deduplicated Percentage of total 1 82.3548776466153 46.75111097323032 2 10.915866574598347 12.393410182402157 3 3.4959809285375987 5.953781865295819 4 1.4181428213750025 3.2201979682361914 5 0.6603787039174764 1.8744146645255093 6 0.3233783301237102 1.1014499660474815 7 0.1943145181132931 0.7721575092739379 8 0.12055863731094592 0.5475085768098014 9 0.07297792715208029 0.3728521420395918 >10 0.361610114030997 3.846394611377986 >50 0.04038805637747528 1.6100874814437984 >100 0.0284422889368114 3.097948750626456 >500 0.003981920451153292 1.4591214290618715 >1k 0.007679418012938492 9.407030286599841 >5k 8.532686681042769E-4 2.627613382378322 >10k+ 5.688457787361846E-4 4.9649202106509405 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 18209 2.9431398124756543 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 12541 2.027015013908352 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 5619 0.9082048770553408 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 5570 0.9002849555433793 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 5085 0.821893895680087 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 4711 0.7614438825071562 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 4605 0.744310991073117 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 3806 0.6151677811127652 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 3514 0.5679715141435252 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 2866 0.46323459292411584 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 2847 0.4601636029500899 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2841 0.45919381664250286 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2735 0.4420609252084637 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 2065 0.3337681208612349 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 1971 0.31857480204237 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 1956 0.31615033627340217 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 1905 0.3079071526589116 No Hit CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC 1864 0.30128027955706627 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 1816 0.2935219890963693 No Hit CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 1804 0.29158241648119504 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 1763 0.2849555433793497 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 1736 0.28059150499520763 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 1732 0.27994498079014957 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 1635 0.26426676881749106 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 1589 0.25683174045932317 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 1397 0.22579857861653516 No Hit CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA 1338 0.21626234659192847 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 1325 0.2141611429254897 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1231 0.19896782410662475 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 1147 0.18539081580040503 No Hit GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA 1040 0.16809629331510134 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 1023 0.1653485654436045 No Hit ACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGAT 888 0.14352837352289421 No Hit CNCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 884 0.14288184931783615 No Hit GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTA 772 0.12477917157620985 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 763 0.12332449211482917 No Hit TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG 707 0.11427315324401602 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 8.081552563226027E-5 0.0 16-17 0.0 0.0 0.0 2.424465768967808E-4 0.0 18-19 0.0 0.0 0.0 3.232621025290411E-4 0.0 20-21 0.0 0.0 0.0 4.848931537935616E-4 0.0 22-23 0.0 0.0 0.0 8.081552563226026E-4 0.0 24-25 0.0 0.0 0.0 0.0017779415639097257 0.0 26-27 0.0 0.0 0.0 0.00299017444839363 0.0 28-29 0.0 0.0 0.0 0.008081552563226027 0.0 30-31 0.0 0.0 0.0 0.023355686907723217 0.0 32-33 0.0 0.0 0.0 0.05891451818591774 0.0 34-35 0.0 0.0 0.0 0.11281847378263533 0.0 36-37 0.0 0.0 0.0 0.1858757089541986 0.0 38-39 0.0 0.0 0.0 0.3036239298004018 0.0 40-41 0.0 0.0 0.0 0.4712353299617096 0.0 42-43 0.0 0.0 0.0 0.5916504631537773 0.0 44-45 0.0 0.0 0.0 0.7272589151647102 0.0 46-47 0.0 0.0 0.0 0.907315906273386 0.0 48-49 0.0 0.0 0.0 1.0731493648707842 0.0 50-51 0.0 0.0 0.0 1.2483574244415243 0.0 52-53 0.0 0.0 0.0 1.460336548174943 0.0 54-55 0.0 0.0 0.0 1.6728005650621554 0.0 56-57 0.0 0.0 0.0 1.890760037692361 0.0 58-59 0.0 0.0 0.0 2.146217914215936 0.0 60-61 0.0 0.0 0.0 2.392543636343065 0.0 62-63 0.0 0.0 0.0 2.6543859393915885 0.0 64-65 0.0 8.081552563226027E-5 0.0 2.9760317314079843 0.0 66-67 0.0 1.6163105126452054E-4 0.0 3.313113288820142 0.0 68-69 0.0 1.6163105126452054E-4 0.0 3.711533830187185 0.0 70-71 0.0 1.6163105126452054E-4 0.0 4.297688837597969 0.0 72-73 0.0 1.6163105126452054E-4 0.0 4.793734533928783 0.0 74-75 0.0 1.6163105126452054E-4 0.0 5.251392855584272 0.0 76-77 0.0 1.6163105126452054E-4 0.0 5.767076724643725 0.0 78-79 0.0 1.6163105126452054E-4 0.0 6.309591348213088 0.0 80-81 0.0 1.6163105126452054E-4 0.0 6.912555984955382 0.0 82-83 0.0 1.6163105126452054E-4 0.0 7.657190238131028 0.0 84-85 0.0 1.6163105126452054E-4 0.0 8.368528494746183 0.0 86-87 0.0 1.6163105126452054E-4 0.0 9.074452111143977 0.0 88 0.0 1.6163105126452054E-4 0.0 9.670062535053734 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGAGT 65 0.0061295005 28.944767 4 CTTGCGG 70 0.0088704545 26.833797 9 AGTACTC 285 0.0 26.405754 5 CCCTTAG 105 0.0016252991 23.818222 1 GAGTACT 4460 0.0 19.741795 12-13 AAGTACT 385 0.0 19.547115 4 TAGTACT 145 6.5619533E-4 19.462862 4 CTATACC 170 8.5696716E-5 19.367455 4 CATGGGG 1775 0.0 18.549112 4 GTACTTT 4880 0.0 18.281796 14-15 AGTGCTC 155 0.0010391992 18.176262 8 GTCGAGT 65 0.008100401 18.053177 18-19 TCGCAGA 65 0.008119298 18.045881 86-87 GCCGGCG 65 0.008124975 18.043692 32-33 AGTACTT 4590 0.0 17.801506 12-13 CCTATAC 160 0.0012726607 17.63536 3 TAGGATC 135 0.009942753 17.420464 5 ACTTTTT 5655 0.0 16.518955 16-17 ACATAGG 295 1.3629851E-6 15.941568 3 TATTAGG 190 0.002671142 15.784361 2 >>END_MODULE