FastQCFastQC Report
Wed 25 May 2016
SRR1295130_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295130_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2814363
Sequences flagged as poor quality0
Sequence length100
%GC44

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG10600.018.1202261
GTACATG102600.017.3317451
TAATACG2653.4496843E-516.9397834
CATGGGG41150.016.9089094
GTGTAGG8900.016.7081741
TACATGG104500.016.539272
GTCTAAC5254.7111826E-1016.5225281
GTATAGG7900.016.470241
GTACCGT2454.579979E-516.388896
GGGTACG2300.002282573416.1633421
GTGTATA13100.015.6081111
ACATGGG103750.015.35864453
GTATAGA10200.015.1861461
GTATTAA17550.014.8279091
TATACAG12700.014.5545785
GTGTAAC6951.1277734E-1014.2640521
GTATAAG10550.014.0950471
GTATATA16400.013.97866251
TATACTG12900.013.9416585
GTATTAT13100.013.7162191
Produced by FastQC (version 0.11.5)