##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295130_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2814363 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.45474944063719 33.0 27.0 33.0 15.0 33.0 2 22.724143616157548 27.0 6.0 33.0 2.0 33.0 3 25.00591075138495 33.0 15.0 33.0 15.0 33.0 4 25.17849296625915 33.0 6.0 37.0 2.0 37.0 5 27.000251922015746 33.0 15.0 37.0 2.0 37.0 6 27.837202947878435 33.0 15.0 37.0 6.0 37.0 7 27.56178147595033 33.0 15.0 37.0 6.0 37.0 8 28.355765762980823 33.0 22.0 37.0 6.0 37.0 9 29.45438772468228 33.0 27.0 37.0 15.0 37.0 10-11 28.95371830144157 35.0 24.5 37.0 10.5 37.0 12-13 27.747706141673977 33.0 15.0 37.0 4.0 37.0 14-15 29.517551218517298 33.0 27.0 37.0 10.5 40.0 16-17 29.956347848518476 35.0 27.0 38.5 6.0 40.0 18-19 30.11107486845158 37.0 27.0 40.0 10.5 40.0 20-21 29.6390069440225 33.0 27.0 38.5 6.0 40.0 22-23 30.39865930585358 35.0 27.0 40.0 15.0 40.0 24-25 30.51440414758153 35.0 27.0 40.0 15.0 40.0 26-27 29.112536122738966 33.0 22.0 38.5 4.0 40.0 28-29 29.788260078746063 33.0 27.0 37.0 10.5 40.0 30-31 30.473491159455975 33.0 27.0 40.0 15.0 40.0 32-33 29.24354249967044 33.0 24.5 37.0 6.0 40.0 34-35 28.87086118599484 33.0 22.0 37.0 6.0 40.0 36-37 29.197477901748993 33.0 22.0 37.0 6.0 40.0 38-39 29.307983724913953 33.0 24.5 37.0 6.0 40.0 40-41 28.355962965687084 33.0 22.0 37.0 6.0 40.0 42-43 28.990207908503628 33.0 22.0 37.0 6.0 40.0 44-45 28.87830745358719 33.0 22.0 37.0 6.0 40.0 46-47 28.554370207396843 33.0 22.0 37.0 6.0 40.0 48-49 28.48439646911219 33.0 22.0 37.0 6.0 40.0 50-51 28.442510081322133 33.0 22.0 37.0 6.0 40.0 52-53 28.60528652487259 33.0 22.0 37.0 6.0 40.0 54-55 28.58128340231875 33.0 22.0 37.0 6.0 40.0 56-57 28.607204187945904 33.0 22.0 37.0 6.0 40.0 58-59 28.44323049301032 33.0 22.0 37.0 6.0 40.0 60-61 28.375861784709365 33.0 22.0 37.0 10.5 40.0 62-63 28.301619940284887 33.0 22.0 37.0 10.5 40.0 64-65 27.88716540830021 33.0 22.0 37.0 6.0 40.0 66-67 28.091794839542732 33.0 22.0 37.0 10.5 40.0 68-69 27.647635361891837 33.0 22.0 37.0 6.0 37.0 70-71 27.776112569700498 33.0 22.0 37.0 10.5 37.0 72-73 27.8094464004821 33.0 22.0 37.0 10.5 37.0 74-75 27.899322333330844 33.0 22.0 37.0 15.0 37.0 76-77 27.65994152140289 33.0 22.0 37.0 15.0 37.0 78-79 27.223971285864685 30.0 22.0 35.0 10.5 37.0 80-81 27.021610041064356 27.0 22.0 33.0 6.0 37.0 82-83 27.124206969747682 27.0 22.0 33.0 15.0 37.0 84-85 27.082894779387022 27.0 22.0 33.0 10.5 37.0 86-87 26.859263179625373 27.0 22.0 33.0 15.0 37.0 88-89 26.80422390430801 27.0 22.0 33.0 15.0 37.0 90-91 26.957014251537558 27.0 22.0 33.0 15.0 37.0 92-93 26.890954542821945 27.0 22.0 33.0 15.0 37.0 94-95 26.76497967746165 27.0 22.0 33.0 15.0 37.0 96-97 26.85498494686009 27.0 22.0 33.0 15.0 37.0 98-99 26.925468214299293 27.0 22.0 33.0 15.0 37.0 100 26.606489639040877 27.0 22.0 33.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.389717521685565 1101 2 11.298178697083593 1101 3 8.408783329817162 1101 4 12.944818617712617 1101 5 10.818791253076068 1101 6 9.960412843853092 1101 7 9.527174642344377 1101 8 9.154505486696532 1101 9 7.329827745320831 1101 10-11 7.792956236098114 1101 12-13 9.254375248800358 1101 14-15 8.09058443524859 1101 16-17 8.247972256439489 1101 18-19 8.245202206643498 1101 20-21 7.221883661985732 1101 22-23 7.443787090288957 1101 24-25 7.463426256494397 1101 26-27 8.54456097912767 1101 28-29 8.191728438445494 1101 30-31 5.566627252901704 1101 32-33 7.027671317839168 1101 34-35 8.725446061738431 1101 36-37 8.292577723733185 1101 38-39 8.345102195567879 1101 40-41 9.095846388756424 1101 42-43 8.671481118308463 1101 44-45 8.603013664275938 1101 46-47 7.39319809065616 1101 48-49 5.494968688167891 1101 50-51 6.797063828182484 1101 52-53 5.855836861956451 1101 54-55 3.9367762032757945 1101 56-57 6.202410784758694 1101 58-59 6.321542235864879 1101 60-61 6.806002542843224 1101 62-63 6.342593926676511 1101 64-65 6.869230265204369 1101 66-67 6.0145902611861395 1101 68-69 6.908004208254422 1101 70-71 6.412767744561258 1101 72-73 5.597101263428797 1101 74-75 4.971398536376899 1101 76-77 5.218639051142354 1101 78-79 5.292994940180044 1101 80-81 5.050184595012542 1101 82-83 4.572033884272283 1101 84-85 4.308822643634727 1101 86-87 4.159391886514488 1101 88-89 4.1862683444333015 1101 90-91 3.877084684902762 1101 92-93 3.804216560756629 1101 94-95 3.7212349444225516 1101 96-97 3.945898041246643 1101 98-99 3.6142845352474566 1101 100 3.84231013697552 1102 1 6.805516669642412 1102 2 10.549807767127515 1102 3 8.183108242363641 1102 4 12.836084544432463 1102 5 10.604497755569358 1102 6 9.558699856025505 1102 7 6.467088273500622 1102 8 7.80870392328568 1102 9 6.445186671156446 1102 10-11 7.446076462820905 1102 12-13 9.077522073792622 1102 14-15 6.1532579134641345 1102 16-17 7.294731935102952 1102 18-19 7.516973835599394 1102 20-21 6.911659227927441 1102 22-23 6.52190788906541 1102 24-25 6.951240518815634 1102 26-27 8.476693070478849 1102 28-29 7.62925496956084 1102 30-31 4.0505813050287 1102 32-33 4.338057026519923 1102 34-35 5.108797449451579 1102 36-37 5.227842378268548 1102 38-39 4.614222323117257 1102 40-41 6.144402630060753 1102 42-43 6.927058013079758 1102 44-45 6.477903632308486 1102 46-47 5.454653434943705 1102 48-49 3.6441762511727305 1102 50-51 4.948890192593275 1102 52-53 5.808990812354764 1102 54-55 5.320573243038208 1102 56-57 6.320702382261846 1102 58-59 6.016387270588407 1102 60-61 6.0287213303261495 1102 62-63 4.254979105559645 1102 64-65 6.211650166827898 1102 66-67 3.7282637415485205 1102 68-69 5.94411692120303 1102 70-71 5.380357593245343 1102 72-73 4.929190059350894 1102 74-75 4.156433898194155 1102 76-77 3.19277879633578 1102 78-79 4.397798782328003 1102 80-81 4.538906184170781 1102 82-83 3.930765535815002 1102 84-85 3.0074796108895363 1102 86-87 3.249065930604978 1102 88-89 3.5021329002455523 1102 90-91 3.3488409168447824 1102 92-93 3.145931802239982 1102 94-95 3.0841304527619933 1102 96-97 3.298950482884212 1102 98-99 3.1461423995242868 1102 100 3.208019586396002 1103 1 6.6764955190094675 1103 2 11.047109485080721 1103 3 8.027043800625048 1103 4 12.28859102301001 1103 5 10.425896259420103 1103 6 9.213579249393462 1103 7 8.153780506135277 1103 8 8.180128963765778 1103 9 7.243628055147344 1103 10-11 7.640853707778653 1103 12-13 8.931226575508695 1103 14-15 8.657179404963603 1103 16-17 8.088744725523462 1103 18-19 7.419570315621186 1103 20-21 8.473550925602428 1103 22-23 6.9234531780504796 1103 24-25 5.2909911273381205 1103 26-27 5.928967060002751 1103 28-29 7.112765272465893 1103 30-31 6.220338530095717 1103 32-33 8.109796220769223 1103 34-35 7.2955268157545206 1103 36-37 4.242296328410216 1103 38-39 6.4790722091624 1103 40-41 6.890337447410072 1103 42-43 7.702091829669385 1103 44-45 5.725896799713521 1103 46-47 6.448401622292309 1103 48-49 5.731066099502975 1103 50-51 6.58188185703877 1103 52-53 6.223147458312685 1103 54-55 4.926554640391355 1103 56-57 5.860492648954978 1103 58-59 5.558686775198847 1103 60-61 4.947640479322281 1103 62-63 2.6507479809114614 1103 64-65 5.073015946083064 1103 66-67 4.053444889269482 1103 68-69 5.209011044748653 1103 70-71 3.8069619438435325 1103 72-73 4.464066646411585 1103 74-75 3.3988043237078323 1103 76-77 3.6635314091957127 1103 78-79 2.985830967971687 1103 80-81 4.194429912731593 1103 82-83 3.498796324175995 1103 84-85 3.354259931043405 1103 86-87 2.8109524912112356 1103 88-89 3.0502036814205162 1103 90-91 2.827257742711655 1103 92-93 2.4079873283756257 1103 94-95 2.901788436706159 1103 96-97 3.1635857862295893 1103 98-99 2.8363728983307226 1103 100 3.0926307484537894 1104 1 6.500533488979464 1104 2 9.842756792973013 1104 3 7.484793238167015 1104 4 12.450931492510858 1104 5 9.535326755720472 1104 6 8.343072813917274 1104 7 4.113271464831875 1104 8 6.726147277261749 1104 9 6.783791253945974 1104 10-11 7.273477226116988 1104 12-13 7.60100298636435 1104 14-15 7.368828276788893 1104 16-17 7.431289540675117 1104 18-19 7.006451836943938 1104 20-21 7.57632332597321 1104 22-23 6.6368110664199875 1104 24-25 6.1816907722928 1104 26-27 8.510329129267536 1104 28-29 7.394383961672588 1104 30-31 6.514276630400737 1104 32-33 7.913361498924079 1104 34-35 8.128691007758682 1104 36-37 6.695998382472336 1104 38-39 7.394269345394324 1104 40-41 7.342346191395901 1104 42-43 7.391034702775642 1104 44-45 6.943097860188036 1104 46-47 6.936131595822477 1104 48-49 5.658650849202193 1104 50-51 5.811278846571465 1104 52-53 6.127067295993779 1104 54-55 4.900219724348229 1104 56-57 6.1380357894778435 1104 58-59 3.9780147534317294 1104 60-61 4.492422853450293 1104 62-63 4.124253502983539 1104 64-65 2.9762422429078477 1104 66-67 3.3193302553609136 1104 68-69 4.175548530833897 1104 70-71 2.541694451914559 1104 72-73 2.610780749613479 1104 74-75 3.331953284122516 1104 76-77 3.9557676024871746 1104 78-79 4.559868542209653 1104 80-81 3.2892347808141835 1104 82-83 3.164864821406251 1104 84-85 3.1460670007061076 1104 86-87 0.9278759006331931 1104 88-89 0.4771882420853615 1104 90-91 2.4908362394080825 1104 92-93 2.6373962308070986 1104 94-95 2.5762214263912533 1104 96-97 3.0115253159733015 1104 98-99 2.6397033982227 1104 100 0.9601528458575821 1105 1 5.6864535201291915 1105 2 -2.8700457275546114 1105 3 0.30323924078070874 1105 4 8.6024999465354 1105 5 8.58064303001581 1105 6 8.006637132585247 1105 7 5.063106761260791 1105 8 5.971919346655717 1105 9 4.9481830287591855 1105 10-11 4.925256603592551 1105 12-13 5.044728451822447 1105 14-15 5.86072359474786 1105 16-17 6.006298125316327 1105 18-19 6.192541124576948 1105 20-21 6.94331536043946 1105 22-23 1.9786985036464806 1105 24-25 3.9668641369176108 1105 26-27 6.881140612705995 1105 28-29 5.913828066736578 1105 30-31 5.212270239933744 1105 32-33 7.087778484606066 1105 34-35 6.136686995769562 1105 36-37 6.282950796497257 1105 38-39 4.025670724014471 1105 40-41 5.272479947345797 1105 42-43 5.524978195556692 1105 44-45 6.1521398053693055 1105 46-47 5.327938067624501 1105 48-49 4.940840775879128 1105 50-51 5.815805374296378 1105 52-53 5.477158917641429 1105 54-55 5.295344547924611 1105 56-57 5.0221455971373 1105 58-59 1.7625379887740458 1105 60-61 4.306367743805119 1105 62-63 -0.9862609722179556 1105 64-65 -0.7176599195634807 1105 66-67 0.5310378205789235 1105 68-69 2.634485609532053 1105 70-71 2.7347205421639913 1105 72-73 2.589632922382499 1105 74-75 2.502082614237551 1105 76-77 1.528980368504115 1105 78-79 3.0456248207918435 1105 80-81 1.4669652040600418 1105 82-83 0.8366443572439799 1105 84-85 1.291682130981549 1105 86-87 -0.4154446311126172 1105 88-89 1.3916455281692954 1105 90-91 1.1001751662480572 1105 92-93 1.393495797307292 1105 94-95 0.9953554489266914 1105 96-97 1.890993977139427 1105 98-99 1.8312337550727307 1105 100 2.013439194355282 1106 1 6.172600417769139 1106 2 10.391755231333295 1106 3 6.75266023254683 1106 4 11.845689772575337 1106 5 9.937092236972301 1106 6 9.365529080920233 1106 7 6.513739009328056 1106 8 7.326875000210283 1106 9 6.729940937956794 1106 10-11 5.471592701497855 1106 12-13 7.826894985044149 1106 14-15 7.812068900314504 1106 16-17 7.668805724488045 1106 18-19 7.189344964212758 1106 20-21 8.148807969978211 1106 22-23 6.134788050098386 1106 24-25 4.934140893776533 1106 26-27 7.681002315946447 1106 28-29 6.185864676594605 1106 30-31 5.364614910174083 1106 32-33 7.237111990346026 1106 34-35 6.689643681183846 1106 36-37 5.87260235843463 1106 38-39 7.053685561670857 1106 40-41 6.5671011212422705 1106 42-43 6.077803023384966 1106 44-45 4.297619935098069 1106 46-47 5.994657177233368 1106 48-49 5.90433659056729 1106 50-51 6.495964488305628 1106 52-53 5.616047691403484 1106 54-55 5.37650282349637 1106 56-57 4.8443662874864195 1106 58-59 4.992469540029962 1106 60-61 4.909269812896024 1106 62-63 5.066186439345444 1106 64-65 5.165330622015205 1106 66-67 3.0944028289186924 1106 68-69 3.111722815428088 1106 70-71 2.8891570380382134 1106 72-73 2.7932798001916623 1106 74-75 2.7838894348044896 1106 76-77 3.565837486948709 1106 78-79 4.090523756096729 1106 80-81 3.682037717360874 1106 82-83 1.9771890361192952 1106 84-85 2.6651648183741905 1106 86-87 3.2864171738481076 1106 88-89 3.3378041563036476 1106 90-91 3.2343699856118135 1106 92-93 2.9183575122129106 1106 94-95 1.3791507362366566 1106 96-97 1.4896098375374862 1106 98-99 2.061593445495056 1106 100 2.9971321108828697 1107 1 6.237743531685798 1107 2 10.482365962825352 1107 3 6.390532823154253 1107 4 11.69655488695211 1107 5 9.610639888020547 1107 6 8.10844746125413 1107 7 3.929281577561948 1107 8 6.624644535343997 1107 9 5.88740171028088 1107 10-11 4.8320346964688525 1107 12-13 6.407597390165172 1107 14-15 6.384719430592764 1107 16-17 7.193273085097445 1107 18-19 6.53858593239606 1107 20-21 5.7224769466481185 1107 22-23 5.9921728896426245 1107 24-25 4.789961308177926 1107 26-27 7.547787267694691 1107 28-29 6.686905711777413 1107 30-31 5.184684640790309 1107 32-33 4.975137455541937 1107 34-35 6.769812283167731 1107 36-37 6.607491923597035 1107 38-39 6.624788416684186 1107 40-41 7.720283503868675 1107 42-43 6.251966262717179 1107 44-45 4.332697381421102 1107 46-47 3.8229672558165326 1107 48-49 4.264093159008365 1107 50-51 5.039294162227879 1107 52-53 4.105427071429286 1107 54-55 5.435352382685327 1107 56-57 5.439006635991515 1107 58-59 3.113346816567084 1107 60-61 2.974611665123085 1107 62-63 0.5703964572523397 1107 64-65 2.2147307073117695 1107 66-67 4.327294315271917 1107 68-69 5.084639810029113 1107 70-71 4.379580345796029 1107 72-73 2.529670181322018 1107 74-75 2.6365482660262636 1107 76-77 3.274259527366361 1107 78-79 4.126870305016464 1107 80-81 2.395857967626924 1107 82-83 1.6394979092493962 1107 84-85 2.3116201271437298 1107 86-87 2.3734050480128026 1107 88-89 2.741622617225307 1107 90-91 1.5105195855655325 1107 92-93 2.4229262331276544 1107 94-95 2.552588497322855 1107 96-97 2.554434313616106 1107 98-99 2.002478780808776 1107 100 0.11399914375971676 1108 1 4.373193977688235 1108 2 -3.2244560640993694 1108 3 -0.032975290469394736 1108 4 6.698382344902807 1108 5 7.033451513707657 1108 6 6.765462701042157 1108 7 4.346471926793374 1108 8 5.081202910644347 1108 9 4.764791526284306 1108 10-11 3.3064170406971556 1108 12-13 2.443251581929765 1108 14-15 3.9765597255139333 1108 16-17 4.532439011169661 1108 18-19 3.866735433729133 1108 20-21 4.561332810510404 1108 22-23 4.043514846786891 1108 24-25 2.870623564193199 1108 26-27 4.61537292978613 1108 28-29 5.990194938596808 1108 30-31 4.424975282967985 1108 32-33 4.647693658431901 1108 34-35 2.98225690316049 1108 36-37 1.0810375606728364 1108 38-39 2.479179977198978 1108 40-41 5.203061555940696 1108 42-43 3.013482341845581 1108 44-45 3.683058462359849 1108 46-47 4.663167710047794 1108 48-49 4.8131850256355335 1108 50-51 2.056006910327021 1108 52-53 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90-91 -4.446669038764568 2212 92-93 -5.660129152379966 2212 94-95 -3.8421029999223713 2212 96-97 -5.731691171020511 2212 98-99 -3.9826790415752598 2212 100 -3.982369921119833 2213 1 -21.185996305712575 2213 2 -17.22267468758115 2213 3 -20.1359712418864 2213 4 -19.21206299922675 2213 5 -21.274888567264004 2213 6 -22.008788311103594 2213 7 -10.731053345338964 2213 8 -10.517234191786986 2213 9 -4.422635447126805 2213 10-11 -17.864808138833723 2213 12-13 -16.481309149336088 2213 14-15 -23.89072772387878 2213 16-17 -18.287629019814016 2213 18-19 -18.80155490126632 2213 20-21 -17.971066979077563 2213 22-23 -20.155081005810402 2213 24-25 -7.800035553185456 2213 26-27 -23.578554195719942 2213 28-29 -24.52065526383234 2213 30-31 -19.82115231209977 2213 32-33 -23.703480430636926 2213 34-35 -18.222900410086222 2213 36-37 -24.126735718679388 2213 38-39 -11.797316393529547 2213 40-41 -17.485351665060847 2213 42-43 -23.673562192567374 2213 44-45 -17.54535929628142 2213 46-47 -17.333556248521873 2213 48-49 -6.427060948049515 2213 50-51 -23.812657057284316 2213 52-53 -18.226321857890643 2213 54-55 -18.130820505569083 2213 56-57 -17.99280628101686 2213 58-59 -18.42077573200585 2213 60-61 -19.73445170496267 2213 62-63 -17.913520156259995 2213 64-65 -17.96446863661193 2213 66-67 -8.985488845436404 2213 68-69 -23.155197588344873 2213 70-71 -17.84549280468641 2213 72-73 -9.741353824185786 2213 74-75 -4.710904173504357 2213 76-77 -3.3001363863312534 2213 78-79 -4.705423127133301 2213 80-81 -3.635898352676005 2213 82-83 -3.70257197097618 2213 84-85 -3.4476668961125547 2213 86-87 -3.407059773945509 2213 88-89 -3.1407465471886304 2213 90-91 -3.1940857055683267 2213 92-93 -3.94693657807829 2213 94-95 -3.968328794539975 2213 96-97 -3.5796231335586164 2213 98-99 -3.864231624277842 2213 100 -3.578086227156259 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 784.0 3 625.0 4 3390.0 5 12561.0 6 21207.0 7 23542.0 8 21341.0 9 21374.0 10 24996.0 11 30711.0 12 38077.0 13 50764.0 14 39667.0 15 44527.0 16 51050.0 17 54521.0 18 55399.0 19 56401.0 20 57556.0 21 55949.0 22 58969.0 23 64794.0 24 71358.0 25 78641.0 26 86536.0 27 92406.0 28 99681.0 29 104206.0 30 110692.0 31 121419.0 32 137971.0 33 167050.0 34 200313.0 35 232730.0 36 249983.0 37 210981.0 38 62100.0 39 91.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.33400919255719 20.4460363202542 14.030360591151192 24.18959389603742 2 15.070218503973035 21.05869991942716 40.25910949412738 23.611972082472427 3 17.77761233007019 26.4963705853964 29.75568202647071 25.970335058062698 4 12.345064470132078 17.71119909528749 39.48245948812565 30.461276946454785 5 13.4011955115315 37.51987596588091 35.90952824470845 13.169400277879154 6 30.337571688062535 37.88481555385651 18.63975973411245 13.137853023968502 7 26.204782403489986 31.749349208463485 23.922488629915392 18.123379758131144 8 24.092372793368234 35.71487733621919 22.342155129270502 17.85059474114207 9 24.677527315938484 17.175245921083008 22.088017878502956 36.05920888447555 10-11 23.214410517854418 27.209280934751117 29.32717641168751 20.24913213570695 12-13 24.22159462695662 24.926257994801553 28.979720060588026 21.872427317653802 14-15 21.70524623254422 26.46323492729875 26.418835168293125 25.412683671863906 16-17 21.300638031683434 29.758277183410403 27.312951572955846 21.628133211950317 18-19 21.303174346869426 28.970158822899545 28.680060305800698 21.046606524430334 20-21 22.632915896419476 28.3622072138074 27.765481848223583 21.239395041549542 22-23 21.73170457513435 28.350764631434487 28.125153588187253 21.792377205243906 24-25 21.972283316270317 28.246249479393736 27.723543923798616 22.057923280537327 26-27 21.90448228923475 28.073203606681435 27.836462148842333 22.18585195524148 28-29 21.73769718463956 28.034950216591692 28.295580165448957 21.93177243331979 30-31 22.31683837402644 28.521076480338497 27.579328651855217 21.58275649377985 32-33 21.540314257784487 28.256635878182145 28.07825325933614 22.124796604697227 34-35 21.63910568615156 27.832497924525274 28.01573923941775 22.51265714990541 36-37 22.255991256760634 28.250925329976877 27.576700547481348 21.916382865781138 38-39 21.98982789634034 28.049248397079307 27.826057744589193 22.134865961991157 40-41 22.06883075039344 27.98859089000441 27.653269447073413 22.289308912528735 42-43 21.937053630291814 28.014350231787354 27.999950673449696 22.04864546447113 44-45 22.252106728127476 27.779674521333437 27.92609764739135 22.04212110314774 46-47 22.097527435763954 28.163401163963204 28.02603857838017 21.71303282189267 48-49 22.00801814017414 27.89088710371465 28.036453210340255 22.064641545770954 50-51 22.15142014139459 27.90678742974531 27.838361512375965 22.103430916484136 52-53 21.68829167198856 27.986120135861142 27.889573127619233 22.436015064531066 54-55 22.120718287085147 28.115849310072644 27.604190300551117 22.159242102291092 56-57 22.227180585514056 27.926058273009506 27.54132815446968 22.30543298700675 58-59 21.972669462860235 27.465321401189346 28.033672134798987 22.528337001151428 60-61 22.33547774858076 27.946573101166095 27.57258036276888 22.14536878748427 62-63 21.894350403998345 27.956663346082106 27.934348871138763 22.214637378780786 64-65 21.844765418126304 27.655977073119587 27.957992916402997 22.541264592351112 66-67 22.021422532028268 27.813507897280086 27.636446676491865 22.528622894199774 68-69 22.45331200657467 27.7815711281894 27.554369294108117 22.210747571127815 70-71 22.381700668187968 27.7760864317305 27.474340580007194 22.367872320074333 72-73 22.186290658192213 27.854522443417707 27.796671563036263 22.162515335353817 74-75 22.38032935550431 27.576494622785553 27.5533575758965 22.489818445813643 76-77 22.339876065536192 27.853599742307722 27.629897550072947 22.17662664208314 78-79 22.35393672921128 27.693560362868023 27.772815544857693 22.179687363063003 80-81 22.438406296971326 27.57831277184371 27.655463740859883 22.327817190325085 82-83 22.017940856000763 27.76343259523416 27.963183171045316 22.255443377719764 84-85 22.17664708254941 27.548786687837516 27.55452555739895 22.72004067221412 86-87 22.3968906321816 27.64543925175259 27.616398256112305 22.3412718599535 88-89 22.217804526507816 27.35122261518242 27.92916338247175 22.501809475838016 90-91 22.34176872261457 27.417706475259184 27.725442765256346 22.51508203686989 92-93 22.069842437710573 27.632423457052198 27.86757003733863 22.4301640678986 94-95 21.98678680480624 27.316550568134534 27.9029008381739 22.793761788885327 96-97 22.210915587315768 27.507065654309958 27.753050468959355 22.528968289414916 98-99 22.193973073335663 27.299237790567137 27.80017283838683 22.706616297710372 100 22.27164253546543 27.221871598438923 27.70265232125828 22.803833544837367 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 364.0 1 406.5 2 362.5 3 286.5 4 326.5 5 430.0 6 619.0 7 868.0 8 1265.5 9 1922.0 10 2717.0 11 3576.0 12 4531.5 13 5515.0 14 6411.0 15 7257.0 16 8021.0 17 8837.5 18 9542.0 19 10191.5 20 10958.0 21 11799.5 22 12857.5 23 14235.0 24 15977.5 25 18220.0 26 21689.0 27 26456.5 28 32580.5 29 39705.5 30 47187.5 31 55277.5 32 63619.5 33 72191.0 34 80164.5 35 87180.5 36 93323.0 37 98599.0 38 102586.5 39 104640.5 40 105565.5 41 105359.5 42 103508.0 43 101020.5 44 98194.5 45 94928.5 46 91458.5 47 88080.0 48 85648.5 49 84418.0 50 84266.5 51 80650.5 52 72642.0 53 65674.5 54 60662.0 55 55995.0 56 51452.0 57 46752.5 58 42302.5 59 37941.5 60 33188.5 61 28394.5 62 23668.5 63 19407.0 64 15777.0 65 12805.0 66 10266.0 67 7921.0 68 6088.5 69 4676.0 70 3478.5 71 2606.0 72 1959.5 73 1443.0 74 1081.5 75 803.0 76 580.0 77 380.5 78 235.5 79 169.5 80 121.0 81 80.0 82 54.0 83 36.0 84 32.5 85 23.5 86 13.0 87 10.0 88 9.5 89 6.0 90 2.0 91 0.5 92 0.0 93 1.0 94 1.5 95 1.0 96 1.0 97 0.5 98 0.5 99 1.0 100 1.5 >>END_MODULE >>Per base N content fail #Base N-Count 1 7.611527013395215 2 24.281586987890332 3 5.981602231126546 4 24.12190609384788 5 12.179843182986701 6 9.469958210792283 7 7.701813874045388 8 8.625042327517809 9 5.033323704156144 10-11 6.7087472369413605 12-13 9.967761799028768 14-15 7.408177267822238 16-17 7.810683980708956 18-19 6.588649012227633 20-21 7.729049166720853 22-23 5.654192440705055 24-25 5.216100410643546 26-27 9.47450630924298 28-29 6.224516880018675 30-31 4.200097855180728 32-33 8.711260771975754 34-35 6.953083166599333 36-37 6.756910888893863 38-39 5.864861782222123 40-41 8.356988775079833 42-43 6.355452370571955 44-45 6.28211428305446 46-47 5.739487052665203 48-49 3.8019971126681242 50-51 6.374319872738521 52-53 4.441484627249577 54-55 4.860726921154094 56-57 4.531913615976333 58-59 3.4881960855795784 60-61 4.1091891841954995 62-63 3.1066887960081906 64-65 4.433294496836407 66-67 2.441138545383094 68-69 5.0558332382851825 70-71 2.487259106234697 72-73 1.513006673268516 74-75 0.7925061550340167 76-77 1.1085456993287648 78-79 1.682494404595285 80-81 2.1327383852047515 82-83 0.7507737985469536 84-85 1.2461079114527869 86-87 0.46726381778043563 88-89 0.7122570897926103 90-91 0.32117392106135567 92-93 0.32303935206652445 94-95 0.5182700312646237 96-97 0.5547614149276409 98-99 0.4584341110226364 100 0.40584672268644806 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2814363.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 93.14981946748722 #Duplication Level Percentage of deduplicated Percentage of total 1 96.33979395712177 89.74034414640809 2 2.70271504150232 5.035148363760067 3 0.4920614099844255 1.3750629452089933 4 0.17851490792457586 0.6651452578171737 5 0.08272758385745883 0.3853029750651851 6 0.04968739208896486 0.27770229617383974 7 0.03643521872903506 0.23757538328076172 8 0.021030801882579907 0.15672123188950468 9 0.015083467443111296 0.12645200423425845 >10 0.07663118393259685 1.223538521102971 >50 0.0032718063480805344 0.20404155392037193 >100 0.0018166030778709254 0.3258417389815669 >500 1.537507382036424E-4 0.09667251115852578 >1k 7.687536905644091E-5 0.15045107099867344 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 3.55320191460732E-5 0.0 0.0 0.0 4 0.0 3.55320191460732E-5 0.0 0.0 0.0 5 0.0 3.55320191460732E-5 0.0 0.0 0.0 6 0.0 3.55320191460732E-5 0.0 0.0 0.0 7 0.0 3.55320191460732E-5 0.0 0.0 0.0 8 0.0 3.55320191460732E-5 0.0 0.0 0.0 9 0.0 3.55320191460732E-5 0.0 1.065960574382196E-4 0.0 10-11 0.0 3.55320191460732E-5 0.0 1.243620670112562E-4 0.0 12-13 0.0 3.55320191460732E-5 0.0 1.77660095730366E-4 0.0 14-15 0.0 3.55320191460732E-5 0.0 1.77660095730366E-4 0.0 16-17 0.0 7.10640382921464E-5 0.0 1.954261053034026E-4 0.0 18-19 0.0 7.10640382921464E-5 0.0 2.131921148764392E-4 0.0 20-21 0.0 7.10640382921464E-5 0.0 2.4872413402251235E-4 0.0 22-23 0.0 7.10640382921464E-5 0.0 2.842561531685856E-4 0.0 24-25 0.0 8.8830047865183E-5 0.0 3.197881723146588E-4 0.0 26-27 0.0 1.065960574382196E-4 0.0 4.263842297528783E-4 0.0 28-29 0.0 1.243620670112562E-4 0.0 7.10640382921464E-4 0.0 30-31 0.0 1.77660095730366E-4 0.0 0.0026293694168094167 0.0 32-33 0.0 1.77660095730366E-4 0.0 0.008136832384450763 0.0 34-35 0.0 1.77660095730366E-4 0.0 0.014976746070069852 0.0 36-37 0.0 1.77660095730366E-4 0.0 0.02520996758413893 0.0 38-39 0.0 2.4872413402251235E-4 0.0 0.04201661264023156 0.0 40-41 0.0 2.4872413402251235E-4 0.0 0.06386880441506657 0.0 42-43 0.0 2.4872413402251235E-4 0.0 0.0854900380654521 0.0 44-45 0.0 2.4872413402251235E-4 0.0 0.11251213862604077 1.77660095730366E-5 46-47 0.0 2.842561531685856E-4 0.0 0.144650849943664 3.55320191460732E-5 48-49 0.0 2.842561531685856E-4 0.0 0.17996967697486074 7.10640382921464E-5 50-51 0.0 2.842561531685856E-4 0.0 0.2172782970782376 7.10640382921464E-5 52-53 0.0 2.842561531685856E-4 0.0 0.25844214125896336 7.10640382921464E-5 54-55 0.0 3.020221627416222E-4 0.0 0.30596621686683634 7.10640382921464E-5 56-57 0.0 3.197881723146588E-4 0.0 0.3613784007251375 7.10640382921464E-5 58-59 0.0 3.197881723146588E-4 0.0 0.4251406090827658 7.10640382921464E-5 60-61 0.0 3.55320191460732E-4 0.0 0.4913189947423271 7.10640382921464E-5 62-63 0.0 3.730862010337686E-4 0.0 0.5604998360197316 7.10640382921464E-5 64-65 0.0 3.908522106068052E-4 0.0 0.6401448569356547 7.10640382921464E-5 66-67 0.0 4.263842297528784E-4 0.0 0.7330255549834901 7.10640382921464E-5 68-69 0.0 4.263842297528784E-4 0.0 0.8389642700675073 7.10640382921464E-5 70-71 0.0 4.263842297528784E-4 0.0 0.9648542139020446 7.10640382921464E-5 72-73 3.55320191460732E-5 4.263842297528784E-4 0.0 1.0943861896990543 7.10640382921464E-5 74-75 3.55320191460732E-5 4.263842297528784E-4 0.0 1.2353950076802458 7.10640382921464E-5 76-77 3.55320191460732E-5 4.263842297528784E-4 0.0 1.3917181259133948 7.10640382921464E-5 78-79 3.55320191460732E-5 4.263842297528784E-4 0.0 1.5647235271356252 7.10640382921464E-5 80-81 3.55320191460732E-5 4.263842297528784E-4 0.0 1.7587816497019042 7.10640382921464E-5 82-83 3.55320191460732E-5 4.263842297528784E-4 0.0 1.9739102596218046 7.10640382921464E-5 84-85 3.55320191460732E-5 4.263842297528784E-4 0.0 2.1988101748068747 7.10640382921464E-5 86-87 3.55320191460732E-5 4.263842297528784E-4 0.0 2.4447983433551395 7.10640382921464E-5 88 3.55320191460732E-5 4.263842297528784E-4 0.0 2.6540997021350834 1.065960574382196E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATTAG 1060 0.0 18.120226 1 GTACATG 10260 0.0 17.331745 1 TAATACG 265 3.4496843E-5 16.939783 4 CATGGGG 4115 0.0 16.908909 4 GTGTAGG 890 0.0 16.708174 1 TACATGG 10450 0.0 16.53927 2 GTCTAAC 525 4.7111826E-10 16.522528 1 GTATAGG 790 0.0 16.47024 1 GTACCGT 245 4.579979E-5 16.38889 6 GGGTACG 230 0.0022825734 16.163342 1 GTGTATA 1310 0.0 15.608111 1 ACATGGG 10375 0.0 15.3586445 3 GTATAGA 1020 0.0 15.186146 1 GTATTAA 1755 0.0 14.827909 1 TATACAG 1270 0.0 14.554578 5 GTGTAAC 695 1.1277734E-10 14.264052 1 GTATAAG 1055 0.0 14.095047 1 GTATATA 1640 0.0 13.9786625 1 TATACTG 1290 0.0 13.941658 5 GTATTAT 1310 0.0 13.716219 1 >>END_MODULE