FastQCFastQC Report
Wed 25 May 2016
SRR1295130_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295130_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2814363
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT64380.22875513926241925No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA47100.16735581017800477No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTACG750.00945051526.4905872
GTCGTAC1705.610012E-520.4688431
GTCGTAA1250.00463849719.8840181
CGTACCC1801.3332756E-418.2868773
GTATCAA103750.018.2070541
GTATAAT13650.017.116281
TATACCG1650.001566646517.0997225
GAGTACT34200.016.95531712-13
TCAACGC114050.016.1214184
TATCAAC115250.016.0753632
CAACGCA113400.016.0479555
ATCAACG114150.016.0246183
CATGGGG50350.015.69031054
CACACCG4802.5465852E-1115.6747455
ACACCGT3302.5931877E-715.6535676
AACGCAG116500.015.599826
GTATACG2254.789836E-415.4653481
TAATACG3051.9363652E-615.4177834
GTACATG133600.014.5111671
TATATCG1950.004772191414.4689965
Produced by FastQC (version 0.11.5)