##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295130_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2814363 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.405665864708993 33.0 33.0 33.0 27.0 33.0 2 30.091735501070758 33.0 33.0 33.0 27.0 33.0 3 30.942552897405204 33.0 33.0 33.0 27.0 33.0 4 35.2152369825783 37.0 37.0 37.0 33.0 37.0 5 35.486101117730726 37.0 37.0 37.0 33.0 37.0 6 35.276629560579075 37.0 37.0 37.0 33.0 37.0 7 35.18275290003457 37.0 37.0 37.0 33.0 37.0 8 35.18618422712351 37.0 37.0 37.0 33.0 37.0 9 35.154709253923535 37.0 37.0 37.0 33.0 37.0 10-11 35.12312253252334 37.0 37.0 37.0 33.0 37.0 12-13 35.02594921124248 37.0 37.0 37.0 33.0 37.0 14-15 36.4880496936607 40.0 37.0 40.0 33.0 40.0 16-17 36.4809134429354 40.0 37.0 40.0 33.0 40.0 18-19 36.50394671902665 40.0 37.0 40.0 33.0 40.0 20-21 36.50253361062521 40.0 37.0 40.0 33.0 40.0 22-23 36.46231633943454 40.0 37.0 40.0 33.0 40.0 24-25 36.42646701935749 40.0 37.0 40.0 33.0 40.0 26-27 36.408443757965834 40.0 37.0 40.0 33.0 40.0 28-29 36.354587876546134 38.5 37.0 40.0 33.0 40.0 30-31 36.26678932319676 37.0 37.0 40.0 33.0 40.0 32-33 36.22247911872065 37.0 37.0 40.0 30.0 40.0 34-35 36.12533369007481 37.0 37.0 40.0 27.0 40.0 36-37 36.111492902656835 37.0 37.0 40.0 27.0 40.0 38-39 36.042528806696225 37.0 37.0 40.0 27.0 40.0 40-41 35.98052276838489 37.0 37.0 40.0 27.0 40.0 42-43 35.885205284464014 37.0 37.0 40.0 27.0 40.0 44-45 35.77461489509349 37.0 37.0 40.0 27.0 40.0 46-47 35.66834946309342 37.0 37.0 40.0 27.0 40.0 48-49 35.55492344093494 37.0 37.0 40.0 27.0 40.0 50-51 35.44947542303534 37.0 37.0 40.0 27.0 40.0 52-53 35.34930710786064 37.0 33.0 40.0 27.0 40.0 54-55 35.21783419551778 37.0 33.0 40.0 27.0 40.0 56-57 35.08870284323664 37.0 33.0 40.0 27.0 40.0 58-59 34.95165211452823 37.0 33.0 40.0 27.0 40.0 60-61 34.782834872402745 37.0 33.0 40.0 27.0 40.0 62-63 34.58961033100563 37.0 33.0 40.0 27.0 40.0 64-65 34.402886727831486 37.0 33.0 40.0 27.0 40.0 66-67 34.17497138784158 37.0 33.0 38.5 27.0 40.0 68-69 33.94986520928537 37.0 33.0 37.0 27.0 40.0 70-71 33.72217141143484 37.0 33.0 37.0 27.0 40.0 72-73 33.503924333854584 37.0 33.0 37.0 22.0 40.0 74-75 33.26864480523656 37.0 33.0 37.0 22.0 40.0 76-77 33.036122383644184 37.0 33.0 37.0 22.0 37.0 78-79 32.801037037510795 37.0 33.0 37.0 22.0 37.0 80-81 32.60090826947341 37.0 33.0 37.0 22.0 37.0 82-83 32.40134197329911 35.0 33.0 37.0 22.0 37.0 84-85 32.23491266052034 33.0 33.0 37.0 22.0 37.0 86-87 32.267340957794005 33.0 33.0 37.0 22.0 37.0 88-89 32.2910090134073 33.0 33.0 37.0 22.0 37.0 90-91 32.31560640898136 33.0 33.0 37.0 22.0 37.0 92-93 32.28786407439267 33.0 33.0 37.0 22.0 37.0 94-95 32.32302407329829 33.0 33.0 37.0 22.0 37.0 96-97 32.291590850220814 33.0 33.0 37.0 22.0 37.0 98-99 32.22248409320333 33.0 33.0 37.0 22.0 37.0 100 31.68387020437662 33.0 33.0 37.0 22.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6836661132198678 1101 2 4.18397646533689 1101 3 2.379097522727765 1101 4 1.179780401487868 1101 5 0.5828564075169211 1101 6 0.5881369497550537 1101 7 0.6627996056699246 1101 8 0.5940654662337153 1101 9 0.6379774616419809 1101 10-11 0.6672062377138346 1101 12-13 0.7163157292462401 1101 14-15 1.0430786224586726 1101 16-17 1.04856228823499 1101 18-19 1.0570627148841254 1101 20-21 1.081596547104425 1101 22-23 1.0219258954364818 1101 24-25 1.025834198345514 1101 26-27 1.0643397389353026 1101 28-29 0.9864995205137603 1101 30-31 0.9704884970028473 1101 32-33 1.0931501046614684 1101 34-35 1.13478262821382 1101 36-37 1.0709393106853398 1101 38-39 1.0630165265238887 1101 40-41 1.1296720521991048 1101 42-43 1.152408150977159 1101 44-45 1.153484400054566 1101 46-47 1.2500873140920632 1101 48-49 1.1731828419708918 1101 50-51 1.1536283670423515 1101 52-53 1.174071094104299 1101 54-55 1.111871099416227 1101 56-57 1.1651695676489382 1101 58-59 1.1203974563323555 1101 60-61 1.1148779739296515 1101 62-63 1.115975070256809 1101 64-65 1.1618939774487131 1101 66-67 1.1213628891393412 1101 68-69 1.1001450966647113 1101 70-71 1.1228015233468724 1101 72-73 1.106516890899627 1101 74-75 1.045347843342043 1101 76-77 1.0114422525291644 1101 78-79 1.0919943202003992 1101 80-81 0.9685489794632431 1101 82-83 1.0409318507080982 1101 84-85 1.1039104869000198 1101 86-87 1.1413246872171214 1101 88-89 1.0100602885427747 1101 90-91 0.9694191285996183 1101 92-93 1.0486307400413608 1101 94-95 1.0581526883970653 1101 96-97 1.0159050028829526 1101 98-99 0.9583192181500486 1101 100 1.0900982149344536 1102 1 0.47025789819429775 1102 2 3.994584371699588 1102 3 2.2537371616527366 1102 4 1.0060813234917418 1102 5 0.3442312296893988 1102 6 0.39198323430454707 1102 7 0.3554150710998485 1102 8 0.41743496016494674 1102 9 0.4960369435975238 1102 10-11 0.5004497573146338 1102 12-13 0.5553469002272422 1102 14-15 0.8482432485039268 1102 16-17 0.815231892403709 1102 18-19 0.844065854869605 1102 20-21 0.8290451223609452 1102 22-23 0.8207301226537851 1102 24-25 0.8356763775133516 1102 26-27 0.8055444991128624 1102 28-29 0.8289522137711529 1102 30-31 0.7822706318484336 1102 32-33 0.9188676235728863 1102 34-35 1.0076243561792424 1102 36-37 0.8629917601248351 1102 38-39 0.7924587247305155 1102 40-41 0.8712010764735041 1102 42-43 0.8366221380005214 1102 44-45 0.865731359255598 1102 46-47 0.8828934443499037 1102 48-49 0.7273642949808874 1102 50-51 0.7631828726023713 1102 52-53 0.8110984043384022 1102 54-55 0.7241867521812893 1102 56-57 0.7485539014635236 1102 58-59 0.7797268214721953 1102 60-61 0.7237022143850851 1102 62-63 0.8356614366709678 1102 64-65 0.7414579222867133 1102 66-67 0.7081553013149673 1102 68-69 0.6701215531879257 1102 70-71 0.785034106590885 1102 72-73 0.7064163089443412 1102 74-75 0.7231920849400169 1102 76-77 0.7190046682952485 1102 78-79 0.6327052580076185 1102 80-81 0.6847111419339242 1102 82-83 0.7139102196446387 1102 84-85 0.7284212501222171 1102 86-87 0.8031257299413355 1102 88-89 0.6939838241600356 1102 90-91 0.7391134435487459 1102 92-93 0.8121502136116732 1102 94-95 0.8080808478834314 1102 96-97 0.802408322443533 1102 98-99 0.8065707944131226 1102 100 0.8112180797318693 1103 1 0.5356398671417466 1103 2 3.972331764123499 1103 3 2.2614762290898263 1103 4 1.087411982809563 1103 5 0.4597509894976781 1103 6 0.3646869058703075 1103 7 0.39238171126902444 1103 8 0.3786715201254012 1103 9 0.49097678411942525 1103 10-11 0.5837357317666729 1103 12-13 0.5460373574909383 1103 14-15 0.8017348700916003 1103 16-17 0.7647829419508838 1103 18-19 0.8528522972352235 1103 20-21 0.7835631302820047 1103 22-23 0.7707939984341792 1103 24-25 0.7706525356434781 1103 26-27 0.7589314712717083 1103 28-29 0.7141099599424265 1103 30-31 0.7242615307239149 1103 32-33 0.7681655477274418 1103 34-35 0.853113510799723 1103 36-37 0.7249483022846093 1103 38-39 0.6831824114902574 1103 40-41 0.7263681750453372 1103 42-43 0.8038099187046299 1103 44-45 0.8512231029587198 1103 46-47 0.7923802012802525 1103 48-49 0.7122720273554535 1103 50-51 0.6466111878904073 1103 52-53 0.6132414132868789 1103 54-55 0.7533303705328862 1103 56-57 0.7254216809464893 1103 58-59 0.6497045295293447 1103 60-61 0.65133192390293 1103 62-63 0.7497885811770075 1103 64-65 0.6858950932941568 1103 66-67 0.7816360792844037 1103 68-69 0.7346406413611604 1103 70-71 0.6696609734016619 1103 72-73 0.705267953921819 1103 74-75 0.6590016223924735 1103 76-77 0.6425923853584194 1103 78-79 0.6341763677201442 1103 80-81 0.5933002327960466 1103 82-83 0.6566677159488918 1103 84-85 0.6228932357783492 1103 86-87 0.6290761170121257 1103 88-89 0.6041273920215957 1103 90-91 0.7171510069695302 1103 92-93 0.7608806531216601 1103 94-95 0.6590502274311945 1103 96-97 0.6573165127510876 1103 98-99 0.7336694777970827 1103 100 0.643302567193313 1104 1 0.42358413911980364 1104 2 3.536303239103571 1104 3 2.158885346908697 1104 4 0.9931815265954853 1104 5 0.44197729931339325 1104 6 0.41226470378584423 1104 7 0.3757025340117508 1104 8 0.33428752534098294 1104 9 0.3757668534615064 1104 10-11 0.44716945331040137 1104 12-13 0.4876172114663646 1104 14-15 0.6554304996488654 1104 16-17 0.7052528903878184 1104 18-19 0.6946956159521207 1104 20-21 0.6515769588513294 1104 22-23 0.6048734640463778 1104 24-25 0.6681558376861432 1104 26-27 0.6509702461333688 1104 28-29 0.7254404230449722 1104 30-31 0.6492289769881481 1104 32-33 0.767817262600822 1104 34-35 0.8119533811349839 1104 36-37 0.663863747067218 1104 38-39 0.7311028314705652 1104 40-41 0.6918254764323422 1104 42-43 0.6838260269087968 1104 44-45 0.6848066481042139 1104 46-47 0.6936285884675257 1104 48-49 0.7025105598871946 1104 50-51 0.6415985321070821 1104 52-53 0.5676772637051855 1104 54-55 0.6657500410240189 1104 56-57 0.679172164421864 1104 58-59 0.6543733078221408 1104 60-61 0.6211867633891046 1104 62-63 0.6947458170089575 1104 64-65 0.8050255821383914 1104 66-67 0.7376320554810931 1104 68-69 0.6693465889898178 1104 70-71 0.5579039684807228 1104 72-73 0.6222419542726811 1104 74-75 0.7028572466835001 1104 76-77 0.5743194123497446 1104 78-79 0.6095665158374572 1104 80-81 0.552180522382379 1104 82-83 0.6427968715309618 1104 84-85 0.6327827976118385 1104 86-87 0.6599823657464654 1104 88-89 0.5635924234470906 1104 90-91 0.6029041652659473 1104 92-93 0.7335582355515555 1104 94-95 0.701335982748283 1104 96-97 0.6179515600462508 1104 98-99 0.6690756800806703 1104 100 0.6353619719752182 1105 1 0.38917796580830455 1105 2 3.640666174837964 1105 3 2.065156956457148 1105 4 0.9077383842737703 1105 5 0.29050375184395705 1105 6 0.2632788542480071 1105 7 0.232744662693797 1105 8 0.18802007861330594 1105 9 0.24093813833707145 1105 10-11 0.21971494429526928 1105 12-13 0.3039281211326781 1105 14-15 0.4715543094028547 1105 16-17 0.4213102932389319 1105 18-19 0.3650600252851959 1105 20-21 0.2660020935401519 1105 22-23 0.3299791327224426 1105 24-25 0.3515660915642229 1105 26-27 0.37350605958430094 1105 28-29 0.2936490277638555 1105 30-31 0.37815870430428333 1105 32-33 0.3974559346853539 1105 34-35 0.46753997050651463 1105 36-37 0.3278837030253712 1105 38-39 0.35090765619535347 1105 40-41 0.3501708066685296 1105 42-43 0.5572965411890962 1105 44-45 0.48251109718675167 1105 46-47 0.4202169644245046 1105 48-49 0.27732249305205414 1105 50-51 0.33474098532359164 1105 52-53 0.2612084932636307 1105 54-55 0.2902726739256636 1105 56-57 0.25893233378521074 1105 58-59 0.28479021089001577 1105 60-61 0.34621708545206786 1105 62-63 0.26326978945515833 1105 64-65 0.263712755929987 1105 66-67 0.261481300349665 1105 68-69 0.31201417581817026 1105 70-71 0.3024655501687832 1105 72-73 0.3574018899521718 1105 74-75 0.17971722620882247 1105 76-77 0.1669897525202515 1105 78-79 0.3380044345056916 1105 80-81 0.24239787140584923 1105 82-83 0.24505024052678692 1105 84-85 0.31086949419621135 1105 86-87 0.2175881253008214 1105 88-89 0.265749532057427 1105 90-91 0.23185938833663045 1105 92-93 0.3385327897696726 1105 94-95 0.30589919678479305 1105 96-97 0.22614227814640486 1105 98-99 0.27537737567155673 1105 100 0.3272212625420714 1106 1 0.44397044697255694 1106 2 3.8571730373748103 1106 3 2.1732969748310254 1106 4 0.9027632581204514 1106 5 0.2884278731829113 1106 6 0.23901398430655263 1106 7 0.1798759738283806 1106 8 0.1875147709200391 1106 9 0.22358607526127372 1106 10-11 0.1716797252548119 1106 12-13 0.20147056361660276 1106 14-15 0.2436985407418817 1106 16-17 0.22735643016435603 1106 18-19 0.24289668914335039 1106 20-21 0.24189544446767286 1106 22-23 0.183382865856629 1106 24-25 0.1773773896898092 1106 26-27 0.25041439553359623 1106 28-29 0.1647348796507302 1106 30-31 0.18097645755037206 1106 32-33 0.28996886387884757 1106 34-35 0.3668827054540813 1106 36-37 0.18220409889894995 1106 38-39 0.1973742502927749 1106 40-41 0.16462796992932738 1106 42-43 0.29215926149507965 1106 44-45 0.2937919308714356 1106 46-47 0.1967782464864669 1106 48-49 0.13822190726017425 1106 50-51 0.06292858002246504 1106 52-53 0.0590661203919538 1106 54-55 0.016912506493973467 1106 56-57 0.12256230341158414 1106 58-59 0.046509239117170864 1106 60-61 0.10179854652798781 1106 62-63 0.17882837252627581 1106 64-65 -0.01839299489699897 1106 66-67 -0.017134533698836663 1106 68-69 -0.036378914152642494 1106 70-71 -0.037852703650322894 1106 72-73 -0.05879604047110121 1106 74-75 -0.08351838383713783 1106 76-77 0.0345861542354271 1106 78-79 0.09217412187943097 1106 80-81 0.05193082354706746 1106 82-83 0.10322629055054477 1106 84-85 0.08392071304585613 1106 86-87 0.14258017994804817 1106 88-89 0.15549898260735517 1106 90-91 0.16416466708381705 1106 92-93 0.22801185541540292 1106 94-95 0.1848941845222356 1106 96-97 0.20162233898204107 1106 98-99 0.2087624511220021 1106 100 0.2625052628948268 1107 1 0.39066956400509767 1107 2 3.5253638218476055 1107 3 2.040956095307717 1107 4 0.9155917272623384 1107 5 0.2881937785037252 1107 6 0.21302861387862748 1107 7 0.11402522129471038 1107 8 0.07820030507969733 1107 9 0.1416363894728363 1107 10-11 0.1566778215538207 1107 12-13 0.08396559583152197 1107 14-15 0.10138797244341191 1107 16-17 0.01670348090774354 1107 18-19 0.07251417305607077 1107 20-21 0.15055082615634774 1107 22-23 0.10947239496307759 1107 24-25 0.16174842168409498 1107 26-27 0.11719950872645057 1107 28-29 0.03745376826605451 1107 30-31 -0.022814006185804203 1107 32-33 0.13732970199161088 1107 34-35 0.28651913220060266 1107 36-37 0.1083324419794991 1107 38-39 0.023769595844768787 1107 40-41 0.07404445530715975 1107 42-43 0.14514585217515474 1107 44-45 0.22737729268897766 1107 46-47 0.1364531912253213 1107 48-49 -0.008400685028959742 1107 50-51 0.1266626323224429 1107 52-53 -0.025285228814077243 1107 54-55 0.007563330063270257 1107 56-57 -0.005947751223963849 1107 58-59 0.12938647798388558 1107 60-61 0.15133242904205702 1107 62-63 0.18838224726409436 1107 64-65 0.0790545524230879 1107 66-67 0.15563654188549947 1107 68-69 0.07400520292699042 1107 70-71 0.0965685914314065 1107 72-73 0.04634294315438581 1107 74-75 0.12032503372991954 1107 76-77 0.12081195604333317 1107 78-79 0.12218141379415215 1107 80-81 0.10301254277067784 1107 82-83 0.09639637647944 1107 84-85 0.15705265768649923 1107 86-87 0.15290711872960117 1107 88-89 0.20664346342168471 1107 90-91 0.11940778248038697 1107 92-93 0.16121875071453928 1107 94-95 0.10345171732575409 1107 96-97 0.14263411232996503 1107 98-99 0.22257792531845055 1107 100 0.23002575626954425 1108 1 0.37934801435235244 1108 2 3.602323105610484 1108 3 2.0921038521294157 1108 4 0.8939564947156811 1108 5 0.36326833167840533 1108 6 0.08088023166605751 1108 7 0.04370034719576665 1108 8 0.08569709583707663 1108 9 0.03109032449801674 1108 10-11 0.019594447076357824 1108 12-13 0.0265649177761631 1108 14-15 0.034086040314782906 1108 16-17 0.03151010346789462 1108 18-19 0.08419219550430768 1108 20-21 -0.010571256724389855 1108 22-23 -0.09717715574295482 1108 24-25 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2211 80-81 0.12767768674653723 2211 82-83 -0.4218983457654417 2211 84-85 -0.07189301488059385 2211 86-87 0.08329975543188084 2211 88-89 0.19695425549281254 2211 90-91 0.17581599957258476 2211 92-93 0.15909962734939143 2211 94-95 0.1866516792584889 2211 96-97 0.11905347769418739 2211 98-99 0.13314308832793742 2211 100 0.01720605917624951 2212 1 0.5924238143084999 2212 2 4.088362393444946 2212 3 2.3838047383251926 2212 4 1.0886245590437582 2212 5 0.5778963915140025 2212 6 0.5301325006374427 2212 7 0.4415091920393053 2212 8 0.48575966970901163 2212 9 0.30690365112828033 2212 10-11 0.3979033480759355 2212 12-13 0.3669756590893556 2212 14-15 0.5085108687714595 2212 16-17 0.5761189098044355 2212 18-19 0.5718998525139156 2212 20-21 0.45517970996170476 2212 22-23 0.3307718117881535 2212 24-25 0.4775237865711617 2212 26-27 0.4364806892529174 2212 28-29 0.3102233658431359 2212 30-31 0.5761919114823542 2212 32-33 0.6729242074743169 2212 34-35 0.4683144088059521 2212 36-37 0.6259325107146694 2212 38-39 0.43215925979425407 2212 40-41 0.6615343329800396 2212 42-43 0.6232988093982499 2212 44-45 0.6933102114633485 2212 46-47 0.5470040391380309 2212 48-49 0.41841290305407597 2212 50-51 0.3671800792406401 2212 52-53 0.2930452584538088 2212 54-55 0.41708647985223735 2212 56-57 0.37922306640231085 2212 58-59 0.5095535903551394 2212 60-61 0.5092599564018769 2212 62-63 0.6401740871111556 2212 64-65 0.7263807544829888 2212 66-67 0.683273877968233 2212 68-69 0.5932032568626155 2212 70-71 0.625572898119124 2212 72-73 0.5371378009048016 2212 74-75 0.577554254767179 2212 76-77 0.6340106114116537 2212 78-79 0.4373814438657888 2212 80-81 0.42042917453277084 2212 82-83 0.4554774732349287 2212 84-85 0.6005681392085549 2212 86-87 0.5534843867215642 2212 88-89 0.6395690039056205 2212 90-91 0.3215503864691698 2212 92-93 0.5244026924614928 2212 94-95 0.5836308583509862 2212 96-97 0.42700921301624106 2212 98-99 0.48189089585898515 2212 100 0.42953859578367215 2213 1 -0.7127519548925321 2213 2 -25.9056930887215 2213 3 -14.82751559115261 2213 4 -6.468642799765373 2213 5 -2.458437249450391 2213 6 -1.467787836627096 2213 7 -1.3599297645814303 2213 8 -1.223533639781543 2213 9 -1.4148813438240424 2213 10-11 -1.4360663593126404 2213 12-13 -1.4401953251052007 2213 14-15 -1.7086234683932844 2213 16-17 -1.6677377911513034 2213 18-19 -1.7016361170147007 2213 20-21 -1.6758273896805989 2213 22-23 -1.9195585762930847 2213 24-25 -1.7329438400579562 2213 26-27 -1.7504223159351397 2213 28-29 -1.7845080821120973 2213 30-31 -1.6626766148990626 2213 32-33 -1.633451514786934 2213 34-35 -1.509625812063284 2213 36-37 -1.6542520734048836 2213 38-39 -1.7498280916887623 2213 40-41 -1.6951054804014092 2213 42-43 -1.7319466566079669 2213 44-45 -1.6278690902854365 2213 46-47 -1.6898339029033949 2213 48-49 -1.72068733367378 2213 50-51 -1.5514369738292828 2213 52-53 -1.7843996270564375 2213 54-55 -1.5861407673762145 2213 56-57 -1.688395584045999 2213 58-59 -1.7356829263838804 2213 60-61 -1.6731194738829487 2213 62-63 -1.6674742461700802 2213 64-65 -1.6752812209470989 2213 66-67 -1.6998762202159696 2213 68-69 -1.7402664815084208 2213 70-71 -1.63828009590976 2213 72-73 -1.6600796185805748 2213 74-75 -1.7589738353484314 2213 76-77 -1.7173102280427663 2213 78-79 -1.756423107867505 2213 80-81 -1.6761699942601709 2213 82-83 -1.8112710090362576 2213 84-85 -1.714233575848315 2213 86-87 -1.6934505440998322 2213 88-89 -1.7063629454717955 2213 90-91 -1.699003864898856 2213 92-93 -1.6261418583834555 2213 94-95 -1.7318904446886378 2213 96-97 -1.8634072569255764 2213 98-99 -1.7221485576873512 2213 100 -1.799944578552111 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 6.0 4 28.0 5 35.0 6 37.0 7 48.0 8 58.0 9 82.0 10 352.0 11 1672.0 12 4754.0 13 8995.0 14 11962.0 15 13416.0 16 14507.0 17 15285.0 18 15731.0 19 17004.0 20 18925.0 21 21013.0 22 23969.0 23 25262.0 24 26426.0 25 27766.0 26 30507.0 27 34983.0 28 41322.0 29 49729.0 30 60067.0 31 74136.0 32 94469.0 33 126711.0 34 174113.0 35 248587.0 36 382056.0 37 645956.0 38 601315.0 39 3076.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.25081848882473 20.435689549981525 10.283062508772082 31.030429452421664 2 12.94862001332595 22.493229103125966 35.87758894133766 28.68056194221042 3 15.209267603361756 26.693642575602368 28.78736680378473 29.309723017251148 4 10.69276230788583 18.609116868238782 37.042482940162294 33.6556378837131 5 11.80402444462714 38.25959265835498 35.02004961956083 14.916333277457053 6 29.97594552297334 39.33454494518239 17.685017893317006 13.004491638527266 7 26.244372359460943 32.98595338077862 22.979789901419874 17.789884358340565 8 24.7058037312316 36.37988136527686 20.74013933732479 18.174175566166745 9 24.945311972359175 16.744773800933448 21.58742797425024 36.722486252457145 10-11 23.199193078963624 27.45276890332499 28.93320240121216 20.414835616499225 12-13 24.18771016841911 24.948191040871315 29.03251434299486 21.831584447714718 14-15 21.755985421205654 26.414129187910817 26.203349511060114 25.626535879823415 16-17 21.044781954994118 29.735801038597288 27.216260694317572 22.003156312091022 18-19 21.102954073435377 28.817992105813588 29.10810194593064 20.970951874820404 20-21 22.231938439186592 28.151412818108863 28.33606042922262 21.280588313481925 22-23 21.513201489915563 28.100609993935066 28.465767606651205 21.920420909498166 24-25 21.649818858220396 27.949408952533535 28.309791180524112 22.090981008721958 26-27 21.447767481867945 27.96835901526239 28.42853886191175 22.155334640957907 28-29 21.349803835579053 27.828427253376482 28.85608040404593 21.965688506998536 30-31 21.952982240527223 28.334514762953805 28.141902343957554 21.570600652561424 32-33 21.118165697724514 28.156571197305496 28.781410523701346 21.94385258126864 34-35 21.260361247889108 27.867646079048846 28.77658435705853 22.095408316003514 36-37 21.867268698102283 28.101115412830314 28.21114474747175 21.820471141595654 38-39 21.609833874104496 28.18095857743842 28.404095528697237 21.805112019759854 40-41 21.653138159912245 27.938448166624674 28.337871657864223 22.070542015598853 42-43 21.520745170961415 27.91745567880866 28.492242658855726 22.069556491374197 44-45 21.823590196975857 27.75974787431525 28.31558139005995 22.101080538648944 46-47 21.747450849391107 28.194276504991688 28.271108619536218 21.787164026080987 48-49 21.687556931851663 28.025398136455898 28.26129991743882 22.02574501425362 50-51 21.78897334112601 27.945784874190295 28.01428767658574 22.250954108097957 52-53 21.493886672872225 28.107887846454748 27.978445461644075 22.419780019028952 54-55 21.794673816910702 28.18635652264 27.724705179490307 22.294264480958994 56-57 21.886747327055538 27.906069714400246 27.813565899461896 22.393617059082324 58-59 21.56603254945114 27.588443279934765 28.326103616445764 22.519420554168327 60-61 21.987756358136654 27.97724048624891 27.79636123540884 22.2386419202056 62-63 21.44743706563597 28.09760459208695 28.08573487748607 22.369223464791016 64-65 21.409184957946817 27.738361187539496 28.18776836312796 22.664685491385725 66-67 21.68942740945117 27.888500939743576 27.932142997395516 22.48992865340974 68-69 21.873427277302465 27.857183984031803 27.840976943352132 22.428411795313608 70-71 21.843463939099966 27.76631458442217 27.754052689540014 22.636168786937848 72-73 21.676953865936238 27.88476062225738 28.03760256545584 22.400682946350543 74-75 21.930123852741477 27.632617114197007 27.773909748996328 22.66334928406519 76-77 21.737578324863 27.905267585256667 27.93846064179521 22.41869344808512 78-79 21.824129122109742 27.929820521390436 27.959085366102855 22.286964990396974 80-81 21.849534141873818 27.812361795955614 27.772809564177404 22.56529449799316 82-83 21.453194487135523 27.905722697302377 28.008897906824277 22.632184908737823 84-85 21.517082350248813 27.692573664487192 27.785322236434588 23.005021748829403 86-87 21.729954657228546 27.853769562065583 27.69682884880531 22.71944693190056 88-89 21.507271714536234 27.54415842041535 28.076218193189256 22.87235167185916 90-91 21.617897211920152 27.640584629368846 27.890996919505966 22.850521239205037 92-93 21.241631670745008 27.933665605818092 27.94054131171057 22.884161411726332 94-95 21.172172001090132 27.524090094590864 28.16999848276553 23.13373942155347 96-97 21.423033464329112 27.768553195420598 27.90736016259585 22.90105317765444 98-99 21.367037279313973 27.588014137550104 27.896078276006282 23.14887030712964 100 21.3692937940425 27.57571370592104 27.87375588997018 23.18123661006628 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 7.0 1 8.5 2 8.0 3 11.0 4 13.5 5 25.5 6 60.0 7 121.5 8 198.0 9 230.0 10 204.0 11 193.5 12 226.0 13 260.5 14 307.5 15 360.5 16 453.0 17 557.0 18 657.5 19 821.0 20 1024.5 21 1409.0 22 2043.5 23 3191.0 24 4800.5 25 6758.5 26 10171.0 27 15452.5 28 22796.0 29 31120.0 30 39303.5 31 48633.0 32 58561.0 33 68437.5 34 78146.0 35 87231.5 36 94739.5 37 101559.0 38 107177.0 39 109944.5 40 112338.5 41 112918.5 42 111376.0 43 110013.0 44 106950.5 45 102932.0 46 100011.0 47 97536.0 48 94093.5 49 92673.5 50 100548.5 51 97150.5 52 80781.5 53 72085.0 54 67128.5 55 62798.0 56 58330.5 57 53495.5 58 48337.5 59 43635.0 60 38492.0 61 32539.0 62 27093.0 63 21985.0 64 17376.0 65 13793.0 66 10733.5 67 8149.5 68 6150.0 69 4569.5 70 3317.0 71 2376.5 72 1794.0 73 1324.5 74 916.0 75 598.5 76 357.5 77 196.5 78 116.5 79 59.5 80 27.0 81 16.0 82 11.0 83 5.5 84 4.5 85 2.0 86 0.5 87 1.5 88 2.0 89 1.0 90 0.5 91 1.0 92 0.5 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.7607760619365732 2 5.343589295339656 3 0.0 4 0.005365334891057053 5 0.16287877576559953 6 0.056176122269941724 7 0.03613606347155644 8 0.0422831027838271 9 0.04025777769250093 10-11 0.04265618898486088 12-13 0.04061309788396167 14-15 0.04391757566454647 16-17 0.03386201424620775 18-19 0.027484016809487616 20-21 0.022207511966295748 22-23 0.022971450377936322 24-25 0.019364950434609892 26-27 0.012596100787282948 28-29 0.018636544042115392 30-31 0.024215071048048886 32-33 0.0014035147562698911 34-35 0.0032156477327196244 36-37 0.004032884173079307 38-39 0.006111507293124589 40-41 0.006466827484585321 42-43 0.006751083637753907 44-45 0.007497256039821445 46-47 0.015474194338114877 48-49 0.024588157249082653 50-51 0.021656765669531612 52-53 0.026862206474431338 54-55 0.023433366626835272 56-57 0.01510110813708111 58-59 0.01415950962971017 60-61 0.011761098337350227 62-63 0.01701983717096906 64-65 0.019364950434609892 66-67 0.020022292788812245 68-69 0.027395186761622434 70-71 0.02730635671375725 72-73 0.023877516866161186 74-75 0.024836881383105164 76-77 0.01852994798467717 78-79 0.014443765782878754 80-81 0.012880356940451532 82-83 0.007745980173843957 84-85 0.010783967810833215 86-87 0.008634280652495788 88-89 0.007301829934518042 90-91 0.007248531905798932 92-93 0.0037841600390567955 94-95 0.0011370246126743422 96-97 8.172364403596836E-4 98-99 4.974482680450247E-4 100 0.10716456974455676 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2814363.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.62443692244159 #Duplication Level Percentage of deduplicated Percentage of total 1 87.7473458463354 68.99085658606865 2 8.650222370927562 13.602377263361742 3 1.8719499106660435 4.415430230193975 4 0.6746841092476272 2.12186632760455 5 0.3070398851110125 1.2070419039792255 6 0.18150676482304498 0.8562520309095564 7 0.10931018194815831 0.6016116053894509 8 0.07826607007492006 0.49229005518183566 9 0.05507883863179772 0.3897488406390407 >10 0.29882614528442264 4.1344961976081915 >50 0.016088004151563334 0.8647621264288696 >100 0.008683425368219196 1.2032878361212214 >500 4.538397411067231E-4 0.20952995413137634 >1k 4.992237149341402E-4 0.6807225703125873 >5k 4.5383974084921836E-5 0.22972647206963553 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 6438 0.22875513926241925 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 4710 0.16735581017800477 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 3.55320191460732E-5 0.0 3 0.0 0.0 0.0 3.55320191460732E-5 0.0 4 0.0 0.0 0.0 3.55320191460732E-5 0.0 5 0.0 7.10640382921464E-5 0.0 3.55320191460732E-5 0.0 6 0.0 7.10640382921464E-5 0.0 3.55320191460732E-5 0.0 7 0.0 7.10640382921464E-5 0.0 7.10640382921464E-5 0.0 8 0.0 7.10640382921464E-5 0.0 1.065960574382196E-4 0.0 9 0.0 7.10640382921464E-5 0.0 1.421280765842928E-4 0.0 10-11 0.0 7.10640382921464E-5 0.0 1.421280765842928E-4 0.0 12-13 3.55320191460732E-5 7.10640382921464E-5 0.0 1.421280765842928E-4 0.0 14-15 3.55320191460732E-5 7.10640382921464E-5 0.0 1.421280765842928E-4 0.0 16-17 3.55320191460732E-5 7.10640382921464E-5 0.0 1.421280765842928E-4 0.0 18-19 3.55320191460732E-5 7.10640382921464E-5 0.0 1.421280765842928E-4 0.0 20-21 3.55320191460732E-5 7.10640382921464E-5 0.0 1.954261053034026E-4 0.0 22-23 3.55320191460732E-5 8.8830047865183E-5 0.0 2.131921148764392E-4 0.0 24-25 3.55320191460732E-5 1.065960574382196E-4 0.0 2.4872413402251235E-4 0.0 26-27 3.55320191460732E-5 1.065960574382196E-4 0.0 4.086182201798417E-4 0.0 28-29 3.55320191460732E-5 1.065960574382196E-4 0.0 7.817044212136104E-4 0.0 30-31 3.55320191460732E-5 1.065960574382196E-4 0.0 0.0034288398475960636 0.0 32-33 3.55320191460732E-5 1.065960574382196E-4 0.0 0.011103755983147875 0.0 34-35 3.55320191460732E-5 1.065960574382196E-4 0.0 0.02099942331532926 0.0 36-37 3.55320191460732E-5 1.065960574382196E-4 0.0 0.036633511739601464 0.0 38-39 3.55320191460732E-5 1.421280765842928E-4 0.0 0.061470393122706635 0.0 40-41 3.55320191460732E-5 1.421280765842928E-4 0.0 0.09197463155961047 0.0 42-43 3.55320191460732E-5 1.421280765842928E-4 0.0 0.12205248576676143 0.0 44-45 3.55320191460732E-5 1.421280765842928E-4 0.0 0.16001844822434064 0.0 46-47 3.55320191460732E-5 1.421280765842928E-4 0.0 0.2065653933056965 0.0 48-49 3.55320191460732E-5 1.421280765842928E-4 0.0 0.25659447626336757 0.0 50-51 3.55320191460732E-5 1.421280765842928E-4 0.0 0.308258032101758 0.0 52-53 3.55320191460732E-5 1.421280765842928E-4 0.0 0.3653935188886437 0.0 54-55 3.55320191460732E-5 1.421280765842928E-4 0.0 0.431554138538632 0.0 56-57 3.55320191460732E-5 1.421280765842928E-4 0.0 0.5082144698462849 0.0 58-59 3.55320191460732E-5 1.421280765842928E-4 0.0 0.5964937714147038 0.0 60-61 3.55320191460732E-5 1.421280765842928E-4 0.0 0.6892323413859549 0.0 62-63 3.55320191460732E-5 1.421280765842928E-4 0.0 0.7840673004868242 0.0 64-65 3.55320191460732E-5 1.421280765842928E-4 0.0 0.8922445327770441 0.0 66-67 3.55320191460732E-5 1.598940861573294E-4 0.0 1.0150254249363 0.0 68-69 3.55320191460732E-5 1.77660095730366E-4 0.0 1.1560697749366375 0.0 70-71 3.55320191460732E-5 1.77660095730366E-4 0.0 1.318397804405473 0.0 72-73 3.55320191460732E-5 1.77660095730366E-4 0.0 1.4798730654148025 0.0 74-75 3.55320191460732E-5 2.3095812444947576E-4 0.0 1.655809858216584 0.0 76-77 3.55320191460732E-5 2.842561531685856E-4 0.0 1.8541673550995377 0.0 78-79 3.55320191460732E-5 2.842561531685856E-4 0.0 2.0734887432786744 0.0 80-81 3.55320191460732E-5 2.842561531685856E-4 0.0 2.3158348798644663 0.0 82-83 3.55320191460732E-5 2.842561531685856E-4 0.0 2.578132245200779 0.0 84-85 3.55320191460732E-5 2.842561531685856E-4 0.0 2.851551132529812 0.0 86-87 3.55320191460732E-5 2.842561531685856E-4 0.0 3.1518322263332768 0.0 88 3.55320191460732E-5 2.842561531685856E-4 0.0 3.4020131731407783 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGTACG 75 0.009450515 26.490587 2 GTCGTAC 170 5.610012E-5 20.468843 1 GTCGTAA 125 0.004638497 19.884018 1 CGTACCC 180 1.3332756E-4 18.286877 3 GTATCAA 10375 0.0 18.207054 1 GTATAAT 1365 0.0 17.11628 1 TATACCG 165 0.0015666465 17.099722 5 GAGTACT 3420 0.0 16.955317 12-13 TCAACGC 11405 0.0 16.121418 4 TATCAAC 11525 0.0 16.075363 2 CAACGCA 11340 0.0 16.047955 5 ATCAACG 11415 0.0 16.024618 3 CATGGGG 5035 0.0 15.6903105 4 CACACCG 480 2.5465852E-11 15.674745 5 ACACCGT 330 2.5931877E-7 15.653567 6 AACGCAG 11650 0.0 15.59982 6 GTATACG 225 4.789836E-4 15.465348 1 TAATACG 305 1.9363652E-6 15.417783 4 GTACATG 13360 0.0 14.511167 1 TATATCG 195 0.0047721914 14.468996 5 >>END_MODULE