FastQCFastQC Report
Wed 25 May 2016
SRR1295129_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295129_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2980218
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG5600.020.0863231
GTACATG83150.019.9158841
TACATGG86050.019.3119772
GTATCAA50600.018.8954331
CATGGGG46400.017.9027634
ACATGGG88700.017.7726423
TACGTCG1750.001476707417.2512575
GTACGGT2352.512283E-416.8325884
TCAACGC55550.016.479794
ATATCGT2454.4772292E-516.4324286
GTATAGA10400.016.2235681
CAACGCA56200.016.205035
TATCAAC57500.016.1887912
ATCAACG55950.016.1581383
TATATAC15000.015.8208373
TCTATAC9600.015.3028933
AACGCAG57000.015.273876
GTGTATA12350.014.6739481
GTATTAC9250.014.1870971
AGTACGG3602.7903181E-514.1257483
Produced by FastQC (version 0.11.5)