##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295129_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2980218
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.909839817087207	27.0	22.0	33.0	6.0	33.0
2	22.057245812219104	27.0	2.0	33.0	2.0	33.0
3	24.328160557382045	27.0	15.0	33.0	6.0	33.0
4	24.270217816280553	33.0	2.0	37.0	2.0	37.0
5	26.312896237792	33.0	15.0	37.0	2.0	37.0
6	27.16829741985318	33.0	15.0	37.0	2.0	37.0
7	26.873207262019086	33.0	15.0	37.0	6.0	37.0
8	27.649587714724223	33.0	15.0	37.0	6.0	37.0
9	28.643339178543314	33.0	22.0	37.0	15.0	37.0
10-11	28.19540651053044	33.0	18.5	37.0	6.0	37.0
12-13	27.003345728399736	33.0	15.0	37.0	4.0	37.0
14-15	28.690030897068603	33.0	18.5	37.0	6.0	40.0
16-17	29.104741498776264	33.0	22.0	37.0	6.0	40.0
18-19	29.28704812869394	33.0	22.0	38.5	6.0	40.0
20-21	28.748422263069347	33.0	22.0	37.0	6.0	40.0
22-23	29.524696515489808	33.0	24.5	37.0	6.0	40.0
24-25	29.658456025700133	33.0	24.5	37.0	6.0	40.0
26-27	28.191022267498553	33.0	18.5	37.0	4.0	40.0
28-29	28.914785931767405	33.0	22.0	37.0	6.0	40.0
30-31	29.62720764051489	33.0	24.5	37.0	10.5	40.0
32-33	28.374443748745897	33.0	22.0	37.0	6.0	40.0
34-35	27.983180928375038	33.0	15.0	37.0	6.0	40.0
36-37	28.271821054701366	33.0	22.0	37.0	6.0	40.0
38-39	28.42065915983327	33.0	22.0	37.0	6.0	40.0
40-41	27.430064679832146	33.0	15.0	37.0	6.0	40.0
42-43	28.062394932182812	33.0	18.5	37.0	6.0	40.0
44-45	27.92732444405074	33.0	22.0	37.0	6.0	40.0
46-47	27.6558198091549	33.0	18.5	37.0	6.0	40.0
48-49	27.582643283142374	33.0	18.5	37.0	6.0	40.0
50-51	27.469432940811714	33.0	22.0	37.0	6.0	40.0
52-53	27.678809738079565	33.0	22.0	37.0	6.0	40.0
54-55	27.634604079298896	33.0	22.0	37.0	6.0	40.0
56-57	27.612774132630566	33.0	22.0	37.0	6.0	40.0
58-59	27.50968536529878	33.0	22.0	37.0	6.0	40.0
60-61	27.46299867996234	33.0	22.0	37.0	6.0	40.0
62-63	27.44223845369701	33.0	22.0	37.0	6.0	40.0
64-65	26.958204735358287	33.0	22.0	37.0	6.0	38.5
66-67	27.14650287327974	33.0	22.0	37.0	6.0	37.0
68-69	26.721085336710267	30.0	22.0	37.0	6.0	37.0
70-71	26.872634149582346	27.0	22.0	37.0	6.0	37.0
72-73	26.932117549789982	27.0	22.0	37.0	10.5	37.0
74-75	27.05683946610617	27.0	22.0	35.0	6.0	37.0
76-77	26.82220562388389	27.0	22.0	33.0	6.0	37.0
78-79	26.397279158772946	27.0	22.0	33.0	6.0	37.0
80-81	26.20067105829171	27.0	22.0	33.0	6.0	37.0
82-83	26.328524792481623	27.0	22.0	33.0	6.0	37.0
84-85	26.286267984422615	27.0	22.0	33.0	6.0	37.0
86-87	26.096097668022942	27.0	22.0	33.0	10.5	37.0
88-89	26.06789872418729	27.0	22.0	33.0	6.0	37.0
90-91	26.22407203097223	27.0	22.0	33.0	10.5	37.0
92-93	26.1525289425136	27.0	22.0	33.0	6.0	37.0
94-95	26.016120800558884	27.0	22.0	33.0	10.5	37.0
96-97	26.102418682123254	27.0	22.0	33.0	6.0	37.0
98-99	26.18109178590291	27.0	22.0	33.0	10.5	37.0
100	25.877146906702798	27.0	22.0	33.0	6.0	37.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	7.218581960385048
1101	2	11.266554204515021
1101	3	8.39057105163823
1101	4	12.722599848241767
1101	5	10.66377560359545
1101	6	9.847704157651592
1101	7	9.351274730321933
1101	8	8.945567359803835
1101	9	7.313727677829338
1101	10-11	7.6990755762774405
1101	12-13	9.100158978245911
1101	14-15	7.852723769081148
1101	16-17	8.104454217926527
1101	18-19	8.140137867391314
1101	20-21	7.285134810345898
1101	22-23	7.362524210501508
1101	24-25	7.286450502509677
1101	26-27	8.522931653757144
1101	28-29	8.199111299439327
1101	30-31	5.488756975823591
1101	32-33	6.89891708986255
1101	34-35	8.685988114575022
1101	36-37	8.243936118897526
1101	38-39	8.12415530871063
1101	40-41	8.86470992357318
1101	42-43	8.514216319554603
1101	44-45	8.312872617691273
1101	46-47	7.22251957251947
1101	48-49	5.426183086547361
1101	50-51	6.832805112450394
1101	52-53	5.680264713967539
1101	54-55	4.113745531796912
1101	56-57	6.1187925287154314
1101	58-59	6.161695429464665
1101	60-61	6.682329573393758
1101	62-63	6.166328388683485
1101	64-65	6.58653676246524
1101	66-67	5.719526781188453
1101	68-69	6.779842838058578
1101	70-71	6.268063991228264
1101	72-73	5.4848553773828215
1101	74-75	4.810530554316266
1101	76-77	5.018453577467184
1101	78-79	5.104843981326507
1101	80-81	4.841004501242782
1101	82-83	4.403145034046485
1101	84-85	4.218973872475054
1101	86-87	4.015173593462425
1101	88-89	4.02586480295772
1101	90-91	3.7491469370735437
1101	92-93	3.8451277968585096
1101	94-95	3.6677345156507855
1101	96-97	3.819735272280834
1101	98-99	3.5451407911373103
1101	100	3.899772461481838
1102	1	6.643072387443983
1102	2	10.431526272873786
1102	3	7.979275422981345
1102	4	12.39496284799161
1102	5	10.440021961847297
1102	6	9.341443271447488
1102	7	6.5607247288739785
1102	8	7.537530364969907
1102	9	6.357722298365534
1102	10-11	7.364489640707067
1102	12-13	8.931304519981179
1102	14-15	6.19148400766019
1102	16-17	7.120073965702137
1102	18-19	7.297980401744599
1102	20-21	6.813708961858236
1102	22-23	6.334921743665294
1102	24-25	6.839224408374196
1102	26-27	8.251909536854921
1102	28-29	7.488840687542609
1102	30-31	4.133295818847479
1102	32-33	4.589547959196274
1102	34-35	5.1256874020737975
1102	36-37	5.1388904698556495
1102	38-39	4.5567933086902705
1102	40-41	6.121635706482934
1102	42-43	6.861931450912532
1102	44-45	6.212601677657943
1102	46-47	5.341350641166546
1102	48-49	3.5848869548135553
1102	50-51	4.8187024776418355
1102	52-53	5.607930505240823
1102	54-55	5.132473879845492
1102	56-57	6.215459525495444
1102	58-59	5.870378655763194
1102	60-61	5.820857363748463
1102	62-63	4.240164983763986
1102	64-65	5.938479197814171
1102	66-67	3.881568871232897
1102	68-69	5.969353175202148
1102	70-71	5.1981626140724835
1102	72-73	4.830626892297669
1102	74-75	4.10567916314055
1102	76-77	3.2439870391493777
1102	78-79	4.130300167823549
1102	80-81	4.255025739391193
1102	82-83	3.728543094897443
1102	84-85	3.007907702243511
1102	86-87	3.081532552980743
1102	88-89	3.2814774216688
1102	90-91	3.2793715811812127
1102	92-93	3.1195407164669184
1102	94-95	3.027335086410826
1102	96-97	3.2333343804608496
1102	98-99	3.022352444296512
1102	100	3.2047896308350268
1103	1	6.460704828094347
1103	2	10.647820171129236
1103	3	7.63181661388704
1103	4	11.562863791897993
1103	5	10.098913750931363
1103	6	8.864736632641481
1103	7	7.900859600188287
1103	8	7.825346328178977
1103	9	6.869417260043637
1103	10-11	7.297405501294488
1103	12-13	8.58065325240673
1103	14-15	8.316462471001014
1103	16-17	7.799872099829425
1103	18-19	7.02005600317376
1103	20-21	8.111715917331193
1103	22-23	6.576129803753435
1103	24-25	5.2475521105798855
1103	26-27	5.821594897566545
1103	28-29	6.880095207868198
1103	30-31	5.977724709107456
1103	32-33	7.8131146917180345
1103	34-35	7.007677175076783
1103	36-37	4.040159343125083
1103	38-39	6.122490110094869
1103	40-41	6.669043706795446
1103	42-43	7.259456904383786
1103	44-45	5.658515289840622
1103	46-47	6.184515780997099
1103	48-49	5.465213228518678
1103	50-51	6.273049418447133
1103	52-53	6.024751231766942
1103	54-55	4.725101158941062
1103	56-57	5.8362561803183866
1103	58-59	5.375920152440742
1103	60-61	4.84342587180511
1103	62-63	2.7205452933154035
1103	64-65	5.004976583467311
1103	66-67	3.9233950990048037
1103	68-69	4.843569943918485
1103	70-71	3.6396659380738043
1103	72-73	4.203439051217309
1103	74-75	3.08524294045052
1103	76-77	3.350621576542437
1103	78-79	2.776692302218496
1103	80-81	3.963765209532184
1103	82-83	3.3024558726391753
1103	84-85	3.2641291429100043
1103	86-87	2.649464633563639
1103	88-89	2.9656765149635866
1103	90-91	2.683712772216783
1103	92-93	2.189494645375486
1103	94-95	2.7688002001700944
1103	96-97	2.864342171723532
1103	98-99	2.7118345568927857
1103	100	2.9079006459001704
1104	1	6.461682833795265
1104	2	9.848743725797345
1104	3	7.4259488721579
1104	4	12.121145521430815
1104	5	9.61367557569449
1104	6	8.300318748749909
1104	7	4.196846322567669
1104	8	6.6549135869413725
1104	9	6.674464585368614
1104	10-11	7.16124454922539
1104	12-13	7.568280014126202
1104	14-15	7.369559325586518
1104	16-17	7.027194219164681
1104	18-19	6.752182261291775
1104	20-21	7.462622214632471
1104	22-23	6.4566784934681
1104	24-25	6.187799628293806
1104	26-27	8.156463177174867
1104	28-29	7.15069274945802
1104	30-31	6.420034966886796
1104	32-33	7.669975316362354
1104	34-35	8.064890962423217
1104	36-37	6.7938245574700495
1104	38-39	7.285494291775851
1104	40-41	7.124461053816372
1104	42-43	7.211896299811915
1104	44-45	6.604383560548587
1104	46-47	6.570073065609112
1104	48-49	5.406856161105672
1104	50-51	5.5509057950485925
1104	52-53	5.926614692045359
1104	54-55	4.772266857372255
1104	56-57	5.953173208023184
1104	58-59	3.8087137584432433
1104	60-61	4.288645790464905
1104	62-63	3.847669771739888
1104	64-65	2.7294354599399924
1104	66-67	3.1663277540753363
1104	68-69	4.020980679813505
1104	70-71	2.4808783328292705
1104	72-73	2.3422526505518526
1104	74-75	3.0106980872694997
1104	76-77	3.7301425521790783
1104	78-79	4.160094237650657
1104	80-81	3.069986981307167
1104	82-83	2.952559198743547
1104	84-85	2.9310845417503337
1104	86-87	0.7042534639816296
1104	88-89	0.3282070841026936
1104	90-91	2.113015281868318
1104	92-93	2.3452664535927745
1104	94-95	2.464745932993985
1104	96-97	2.83378052101914
1104	98-99	2.428100514536208
1104	100	0.7937178688909441
1105	1	5.694358535245861
1105	2	-2.9137920209930996
1105	3	0.4085410143122026
1105	4	8.331164463744663
1105	5	8.507105804796044
1105	6	8.086999395647943
1105	7	5.20709806221404
1105	8	5.8765889715197375
1105	9	4.8180431486121265
1105	10-11	5.041362223528349
1105	12-13	5.078552861613396
1105	14-15	5.811063807947281
1105	16-17	6.061106180626705
1105	18-19	6.076293284460622
1105	20-21	7.012659458895637
1105	22-23	2.167535524002286
1105	24-25	4.13613958827548
1105	26-27	6.7716899126868775
1105	28-29	5.913608488306924
1105	30-31	5.268540084901659
1105	32-33	7.0501081531734116
1105	34-35	6.194025397090179
1105	36-37	6.191052937560389
1105	38-39	4.145295338281716
1105	40-41	5.457954232151785
1105	42-43	5.573959539055799
1105	44-45	6.191489903454755
1105	46-47	5.392181937509594
1105	48-49	5.06027850213836
1105	50-51	5.995800657504528
1105	52-53	5.61360035870112
1105	54-55	5.33187077854198
1105	56-57	5.1431080143307355
1105	58-59	1.9138791959197938
1105	60-61	4.3650963911790335
1105	62-63	-0.6772522100903089
1105	64-65	-0.27991493161809444
1105	66-67	0.9904280196292881
1105	68-69	2.9243532748341146
1105	70-71	3.0295329244020976
1105	72-73	2.6855766084222346
1105	74-75	2.6585210451909305
1105	76-77	1.7492545308023608
1105	78-79	3.1785834585841855
1105	80-81	1.6976507163434214
1105	82-83	1.0680697825179344
1105	84-85	1.450574335447289
1105	86-87	-0.21973300001072715
1105	88-89	1.607592145734916
1105	90-91	1.3488720164089578
1105	92-93	1.7267657366518492
1105	94-95	1.0672134370401842
1105	96-97	1.973846894866501
1105	98-99	1.9604924441098568
1105	100	2.1549275391337623
1106	1	6.176709196992476
1106	2	10.337681103991411
1106	3	6.726576028269072
1106	4	11.70788015989719
1106	5	9.924944013250165
1106	6	9.31395937143651
1106	7	6.417371099184287
1106	8	7.378377693728346
1106	9	6.878599065331006
1106	10-11	5.5643625461972555
1106	12-13	7.824252365994958
1106	14-15	7.743945796989561
1106	16-17	7.7457807593985954
1106	18-19	7.060401840894013
1106	20-21	8.038371101535692
1106	22-23	5.923030795738814
1106	24-25	4.895906267867257
1106	26-27	7.550872749304514
1106	28-29	6.181306352058545
1106	30-31	5.241111375579784
1106	32-33	7.070461489392152
1106	34-35	6.850080270329361
1106	36-37	5.935643740069747
1106	38-39	6.938620535218636
1106	40-41	6.471934807433641
1106	42-43	6.118081933454327
1106	44-45	4.532311399962598
1106	46-47	5.961390324798398
1106	48-49	5.726797347729651
1106	50-51	6.400670855238737
1106	52-53	5.425836644459103
1106	54-55	5.122742452692943
1106	56-57	4.847155744296167
1106	58-59	4.948557917570177
1106	60-61	4.809707845499606
1106	62-63	4.836802198545701
1106	64-65	5.1066413025128625
1106	66-67	3.113243402199707
1106	68-69	3.3409872699182337
1106	70-71	2.799861691223626
1106	72-73	2.6539746061227483
1106	74-75	2.7454153181808394
1106	76-77	3.5230538908215046
1106	78-79	4.1621165804596565
1106	80-81	3.6335811004646033
1106	82-83	1.886338875367727
1106	84-85	2.589788682973964
1106	86-87	3.286380724632295
1106	88-89	3.2659124976317635
1106	90-91	3.050080084783726
1106	92-93	2.819792208268627
1106	94-95	1.335080520481263
1106	96-97	1.3973872561405223
1106	98-99	1.9999823243468775
1106	100	2.85781671500634
1107	1	6.089685016911311
1107	2	10.42416029856124
1107	3	6.195454335337985
1107	4	11.361229021975607
1107	5	9.442466941899706
1107	6	8.03163752119453
1107	7	3.7274374537700794
1107	8	6.224696904475085
1107	9	5.508395328421134
1107	10-11	4.44990882190071
1107	12-13	6.058847979580577
1107	14-15	6.072592609856766
1107	16-17	6.96913087833212
1107	18-19	6.3448299185794035
1107	20-21	5.628404170856481
1107	22-23	5.793534789662122
1107	24-25	4.5974383822070415
1107	26-27	7.14515083929474
1107	28-29	6.299472252156374
1107	30-31	4.92192040714356
1107	32-33	5.014442646118802
1107	34-35	6.737109545500125
1107	36-37	6.591076638088051
1107	38-39	6.376742264273123
1107	40-41	7.496280255105429
1107	42-43	6.074892963646615
1107	44-45	3.9958797678551328
1107	46-47	3.3646698508674895
1107	48-49	3.925812517662333
1107	50-51	4.797073726958608
1107	52-53	3.9445581133256695
1107	54-55	5.384930190623429
1107	56-57	5.29580893398645
1107	58-59	3.074904894564952
1107	60-61	2.7913631843822735
1107	62-63	0.44220079427133996
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2103	10-11	7.508683217843444
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2104	3	7.7357363547919356
2104	4	12.057706578128045
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2206	100	1.7036918238077412
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2207	3	7.6795140637717125
2207	4	11.852298320129822
2207	5	10.383461784812969
2207	6	9.202909827940978
2207	7	5.723419565026035
2207	8	6.8352742711157966
2207	9	6.168812420934525
2207	10-11	6.867885050108956
2207	12-13	8.737316864070262
2207	14-15	8.218076077790808
2207	16-17	7.409217499533593
2207	18-19	4.134546406065674
2207	20-21	7.516388303906769
2207	22-23	6.390657346627496
2207	24-25	4.379769270683052
2207	26-27	4.029016843920534
2207	28-29	5.156120362510393
2207	30-31	5.5159268348111254
2207	32-33	6.916204826395727
2207	34-35	6.332176977749732
2207	36-37	6.7867920417032614
2207	38-39	6.86533156860725
2207	40-41	4.107033668772345
2207	42-43	5.662954330712047
2207	44-45	4.009937183791994
2207	46-47	5.581716795349273
2207	48-49	2.380444853526754
2207	50-51	5.221017534278907
2207	52-53	2.839754954928182
2207	54-55	3.183219693557895
2207	56-57	5.251141158063618
2207	58-59	3.633438132283267
2207	60-61	3.5865651411165125
2207	62-63	4.279293169474055
2207	64-65	3.478504198754056
2207	66-67	3.648361990755067
2207	68-69	3.5374323173151545
2207	70-71	3.6163663509350883
2207	72-73	3.7750770915932748
2207	74-75	3.3803936380888153
2207	76-77	3.9712604208874502
2207	78-79	4.1148751558450165
2207	80-81	4.122967153362126
2207	82-83	3.748022160659797
2207	84-85	3.3216582991704477
2207	86-87	3.434240331548647
2207	88-89	3.17661920631436
2207	90-91	1.4454461536535383
2207	92-93	-0.6756681999617093
2207	94-95	1.3835825817173983
2207	96-97	-0.9036261055756576
2207	98-99	0.7026667738225783
2207	100	2.06701784410172
2208	1	1.0510461401870757
2208	2	8.527125661262172
2208	3	6.592735077482121
2208	4	11.656341280879861
2208	5	9.930809070903383
2208	6	9.066591947011808
2208	7	7.467882254504204
2208	8	7.5665072151031545
2208	9	6.44256071606539
2208	10-11	6.506629099417697
2208	12-13	6.868470255632385
2208	14-15	7.695747380289358
2208	16-17	6.972470890139075
2208	18-19	6.302017073335737
2208	20-21	6.349740225015598
2208	22-23	5.686496521484489
2208	24-25	6.010572427067917
2208	26-27	7.813777346656327
2208	28-29	6.861328891483115
2208	30-31	5.947201353591922
2208	32-33	8.23951734760287
2208	34-35	6.262631812237348
2208	36-37	6.673613854715672
2208	38-39	7.0881630048323565
2208	40-41	7.41331434958375
2208	42-43	6.853086689177157
2208	44-45	5.498344903369183
2208	46-47	4.356908921362578
2208	48-49	4.375294750365818
2208	50-51	5.76530220574335
2208	52-53	5.311886774941637
2208	54-55	6.076145040593698
2208	56-57	6.2801922648960975
2208	58-59	5.554050812858826
2208	60-61	4.95622884701492
2208	62-63	3.162069029796026
2208	64-65	3.1087385814108366
2208	66-67	2.6834313271436585
2208	68-69	5.260547255756347
2208	70-71	5.038298828648308
2208	72-73	4.504269488296654
2208	74-75	2.000327741211482
2208	76-77	3.285980240554956
2208	78-79	4.001155991378294
2208	80-81	3.2599806726376386
2208	82-83	1.1076555693789665
2208	84-85	1.7547052131864724
2208	86-87	1.036779717784043
2208	88-89	0.6573878870531367
2208	90-91	1.8376932014429812
2208	92-93	0.7225392275083813
2208	94-95	-1.248396595158308
2208	96-97	1.1287242134540243
2208	98-99	0.17393262720207403
2208	100	1.5824016646641716
2209	1	5.438204254777009
2209	2	9.66435729650663
2209	3	5.676271924511319
2209	4	-3.1291074910630208
2209	5	0.7381616343953716
2209	6	4.565079296359116
2209	7	2.354260746064856
2209	8	5.34646809699089
2209	9	5.142055713811519
2209	10-11	5.223686841852565
2209	12-13	7.28753031232171
2209	14-15	6.762313359216446
2209	16-17	6.375536247690338
2209	18-19	5.433681718872801
2209	20-21	6.155860404671781
2209	22-23	5.522829296391283
2209	24-25	5.337460114468971
2209	26-27	7.099732933428729
2209	28-29	6.363738132563672
2209	30-31	4.223331487329354
2209	32-33	6.311649178888047
2209	34-35	5.790112721888857
2209	36-37	6.42784931964276
2209	38-39	7.03716769727227
2209	40-41	7.368390883136485
2209	42-43	6.919795758620932
2209	44-45	6.671161912196016
2209	46-47	6.836819190008317
2209	48-49	5.255914495123562
2209	50-51	4.7795913957001765
2209	52-53	5.0635610249746925
2209	54-55	5.548043233989727
2209	56-57	5.259645821942929
2209	58-59	3.306527432907579
2209	60-61	4.262943489230921
2209	62-63	4.362930786207219
2209	64-65	5.063297644966731
2209	66-67	4.604139786106206
2209	68-69	3.904184274767129
2209	70-71	3.8026681119415287
2209	72-73	1.1072776240077609
2209	74-75	2.404357056186708
2209	76-77	3.408982438584278
2209	78-79	3.5432327443077583
2209	80-81	3.747119011715906
2209	82-83	3.0931343960769517
2209	84-85	3.083815628351541
2209	86-87	1.6642547994181882
2209	88-89	2.104640582654831
2209	90-91	2.4127810036133504
2209	92-93	2.4822096299006553
2209	94-95	0.9470384007307118
2209	96-97	0.27531641319152556
2209	98-99	0.41538258572354536
2209	100	0.43742317205955317
2210	1	1.5169280160268919
2210	2	-16.97558709644981
2210	3	-7.4955355679448346
2210	4	-7.767746655815358
2210	5	-1.4589653643030331
2210	6	2.633016433167377
2210	7	-7.9917367912956685
2210	8	-8.011567355061576
2210	9	-3.2359392115699173
2210	10-11	-1.2583915116635787
2210	12-13	3.1363384358109236
2210	14-15	-4.961330893331475
2210	16-17	-1.4150829260516566
2210	18-19	-0.6272248164921379
2210	20-21	2.109091367165572
2210	22-23	2.157021801006902
2210	24-25	1.6025685979837796
2210	26-27	3.7405267613104343
2210	28-29	3.0756534423628388
2210	30-31	2.378256063504999
2210	32-33	4.230061625612606
2210	34-35	2.1281515560442905
2210	36-37	3.843381913012962
2210	38-39	2.9011561922657343
2210	40-41	3.817875632194699
2210	42-43	1.8955682031200354
2210	44-45	3.079345005487891
2210	46-47	2.5027168162405538
2210	48-49	2.5781795670774343
2210	50-51	1.8574830278503605
2210	52-53	1.9457274888615927
2210	54-55	2.2280920329039375
2210	56-57	2.506664812963521
2210	58-59	1.5015714514901148
2210	60-61	2.3953170959435894
2210	62-63	1.536058587240003
2210	64-65	2.30231876773707
2210	66-67	2.352090890560479
2210	68-69	2.9330382689428838
2210	70-71	2.0844851357787633
2210	72-73	0.659496254270703
2210	74-75	0.026934101517138487
2210	76-77	-0.5956961371843441
2210	78-79	-0.0687196368537819
2210	80-81	0.5440423082560599
2210	82-83	0.5649961778742103
2210	84-85	-1.3072616812514397
2210	86-87	-1.059629479952303
2210	88-89	-1.5347811363026587
2210	90-91	-1.8198157633304746
2210	92-93	-1.3521764012820405
2210	94-95	-0.571400824635937
2210	96-97	-0.11302375023285904
2210	98-99	-0.4102289965882058
2210	100	-0.2910808403444207
2211	1	0.9448895903552739
2211	2	-16.97558709644981
2211	3	-7.965180053835407
2211	4	-18.734760873824836
2211	5	-20.990908492265117
2211	6	-14.742134880689115
2211	7	-12.916786036961922
2211	8	-17.14344661056419
2211	9	-16.985429637088245
2211	10-11	-10.946417086196352
2211	12-13	-22.044799004947368
2211	14-15	-17.546024241653544
2211	16-17	-14.598642282728246
2211	18-19	-18.880034310494512
2211	20-21	-9.59122684542752
2211	22-23	-5.476884383124624
2211	24-25	-2.0576509955318905
2211	26-27	-16.99856650525489
2211	28-29	-3.6896736485021187
2211	30-31	-6.123528022032996
2211	32-33	-1.7109481979716605
2211	34-35	-8.601562167592352
2211	36-37	-9.516117965629366
2211	38-39	-4.132624929590143
2211	40-41	-8.377393206987225
2211	42-43	-3.0349133952243896
2211	44-45	-0.41777792344761266
2211	46-47	0.9300776062151321
2211	48-49	0.6940403762137493
2211	50-51	-6.71405463773899
2211	52-53	-1.5295635779362229
2211	54-55	-0.43135368189275525
2211	56-57	-9.437797444800179
2211	58-59	-3.396080469154743
2211	60-61	-1.6652301632891735
2211	62-63	-0.5689560395532176
2211	64-65	-4.879330674656359
2211	66-67	-3.5849215390201365
2211	68-69	0.07550080774540291
2211	70-71	-0.1500264315236457
2211	72-73	-0.9676568224656705
2211	74-75	-1.5576265198341481
2211	76-77	-1.6493975462107997
2211	78-79	-0.8650906591963654
2211	80-81	-0.8191335581738564
2211	82-83	-1.3481909301085686
2211	84-85	-1.3850619137471902
2211	86-87	-1.7348381512774012
2211	88-89	-2.466592431508392
2211	90-91	-2.244342660023161
2211	92-93	-2.029459522345899
2211	94-95	-2.067853220491891
2211	96-97	-2.879782606149366
2211	98-99	-2.667828925051051
2211	100	-1.9917308064920185
2212	1	-20.972361083499173
2212	2	-16.97558709644981
2212	3	-19.763023719603602
2212	4	-18.734760873824836
2212	5	-20.990908492265117
2212	6	-21.750490675837362
2212	7	-22.079319729684293
2212	8	-22.58711237875826
2212	9	-23.882195135740535
2212	10-11	-11.15982206917887
2212	12-13	-22.044799004947368
2212	14-15	-23.393463594753275
2212	16-17	-23.812523684345496
2212	18-19	-18.384725210745174
2212	20-21	-23.484083988284663
2212	22-23	-18.535737721241418
2212	24-25	-25.021128084480676
2212	26-27	-17.104977799574684
2212	28-29	-17.960588745202003
2212	30-31	-6.348213753312589
2212	32-33	-17.024527604224467
2212	34-35	-13.599963208939402
2212	36-37	-17.396425484003995
2212	38-39	-23.483298784037583
2212	40-41	-10.111547596364165
2212	42-43	-13.968804851552486
2212	44-45	-16.900293111725613
2212	46-47	-16.938961083179272
2212	48-49	-17.48466664933843
2212	50-51	-8.933438279243706
2212	52-53	-2.179927884184778
2212	54-55	-23.268685218280897
2212	56-57	-23.133619547226054
2212	58-59	-12.410297971904447
2212	60-61	-6.403092404309561
2212	62-63	-3.3263730409835333
2212	64-65	-17.063674002907266
2212	66-67	-17.338204425713457
2212	68-69	-14.368698851432942
2212	70-71	-9.548542063530672
2212	72-73	-17.2041701744608
2212	74-75	-6.232390729784068
2212	76-77	-10.699509186544823
2212	78-79	-4.85737237047983
2212	80-81	-2.4077064737095633
2212	82-83	-2.8404905793738173
2212	84-85	-5.087241968599653
2212	86-87	-4.916666149634658
2212	88-89	-3.2540972110364343
2212	90-91	-4.063005477891277
2212	92-93	-5.357801769597376
2212	94-95	-3.663653254479648
2212	96-97	-5.316945737932166
2212	98-99	-3.791720310571325
2212	100	-3.813134359156514
2213	1	-20.972361083499173
2213	2	-16.97558709644981
2213	3	-19.763023719603602
2213	4	-18.734760873824836
2213	5	-20.990908492265117
2213	6	-21.750490675837362
2213	7	-10.511615449528652
2213	8	-10.457540394322463
2213	9	-4.926164007335867
2213	10-11	-17.475610977981095
2213	12-13	-16.143437137243087
2213	14-15	-23.393463594753275
2213	16-17	-17.88509177773071
2213	18-19	-18.42524443771404
2213	20-21	-17.56423963030801
2213	22-23	-19.576119807416944
2213	24-25	-7.817042481367835
2213	26-27	-23.087245750537907
2213	28-29	-23.982261249580286
2213	30-31	-19.304644889894597
2213	32-33	-23.152592402823416
2213	34-35	-17.647767082272416
2213	36-37	-23.482020930588934
2213	38-39	-11.505477772364431
2213	40-41	-16.902236673483287
2213	42-43	-23.043673503041102
2213	44-45	-17.010815984424887
2213	46-47	-16.8161509064806
2213	48-49	-6.332591819870025
2213	50-51	-23.12564493423495
2213	52-53	-17.553254936867287
2213	54-55	-17.43775136614082
2213	56-57	-17.289650675630725
2213	58-59	-17.791263851956508
2213	60-61	-19.053153223270083
2213	62-63	-17.34894334906479
2213	64-65	-17.411003931287404
2213	66-67	-8.62862978364241
2213	68-69	-22.439189219208775
2213	70-71	-17.239137782693582
2213	72-73	-9.44054689766468
2213	74-75	-4.392880382042716
2213	76-77	-3.2270596645784444
2213	78-79	-4.618703777595478
2213	80-81	-3.4233492125730507
2213	82-83	-3.4607151836246715
2213	84-85	-3.329471281635012
2213	86-87	-3.429192981529006
2213	88-89	-2.9489925070220444
2213	90-91	-2.8459158610677626
2213	92-93	-3.721634420350032
2213	94-95	-3.8954843992389954
2213	96-97	-3.648057958284202
2213	98-99	-4.046277643941
2213	100	-3.645871996041766
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	847.0
3	886.0
4	4921.0
5	15698.0
6	22673.0
7	28423.0
8	27878.0
9	28576.0
10	33216.0
11	39597.0
12	49829.0
13	64751.0
14	53684.0
15	58036.0
16	64651.0
17	67025.0
18	67106.0
19	66379.0
20	64860.0
21	63199.0
22	64800.0
23	69605.0
24	76304.0
25	82968.0
26	90038.0
27	96874.0
28	103768.0
29	107262.0
30	112602.0
31	122921.0
32	139756.0
33	166512.0
34	199224.0
35	229647.0
36	242861.0
37	197861.0
38	54921.0
39	59.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.343966224932565	20.060689574293423	13.633824909112233	24.96151929166178
2	15.254901588498473	20.742339028854627	40.13843927617741	23.864320106469496
3	18.000266726003016	26.502427673937166	29.29000198067423	26.207303619385584
4	12.969136861167597	17.519234325832457	37.92721115821517	31.58441765478477
5	13.546274491726107	37.294092079864164	35.64508550096013	13.5145479274496
6	30.81824625792271	37.95614234653299	18.202524362751642	13.023087032792649
7	26.79952976576117	31.526126186380477	23.19321239880832	18.48113164905003
8	25.170125396046895	35.51553968532257	21.289439168750928	18.024895749879605
9	25.156184744851927	17.193185883721295	21.42047228157298	36.2301570898538
10-11	23.860040059410295	26.911488253252223	28.683714392515242	20.544757294822237
12-13	24.62777111874079	24.522806637366905	28.54520357466135	22.30421866923096
14-15	21.989986457768353	26.058721001712282	25.97362763192532	25.977664908594043
16-17	21.841865728779894	29.395899320161746	26.641557193436576	22.12067775762178
18-19	21.86818930851234	28.58193994295145	28.083121492767347	21.466749255768864
20-21	23.06928421580515	27.87654169384293	27.308707900075664	21.745466190276254
22-23	22.39240079588046	27.771561512530184	27.606518330280387	22.22951936130897
24-25	22.508219724563162	27.801565760109664	27.21124736611164	22.47896714921554
26-27	22.340639569714813	27.744358524074496	27.339347239344637	22.57565466686605
28-29	22.28584630881432	27.666874974921136	27.6831413724485	22.364137343816044
30-31	22.683739580150814	28.047343058145245	27.130285646219075	22.13863171548486
32-33	22.09616381544842	27.85792046211953	27.55387691624084	22.492038806191218
34-35	22.16869824284263	27.454508104925434	27.452069958782594	22.92472369344934
36-37	22.64260124504502	27.862327625741827	27.084932775742054	22.410138353471094
38-39	22.39203729294651	27.700197743882924	27.346845353664506	22.560919609506055
40-41	22.524086702882265	27.660413094584126	27.090211615256422	22.72528858727719
42-43	22.397911377850324	27.736252835106168	27.393632227547986	22.472203559495522
44-45	22.637626194516944	27.459371224357653	27.42130472127472	22.481697859850684
46-47	22.48819071265237	27.654959757499736	27.546657245010316	22.310192284837576
48-49	22.459571975041865	27.50051771278521	27.451724815769584	22.58818549640334
50-51	22.62242248490444	27.52741330258593	27.339251630204696	22.51091258230493
52-53	22.266562467286906	27.499138978188213	27.413769393732963	22.820529160791917
54-55	22.569942426470327	27.568947942305712	27.29360013922778	22.56750949199618
56-57	22.625909238967846	27.5050643904231	27.16367744974001	22.705348920869042
58-59	22.43664993523316	27.133676523137396	27.526866373727	22.90280716790245
60-61	22.670088357382987	27.523360801340157	27.17759161373179	22.628959227545067
62-63	22.358918031143215	27.624732709118305	27.392633934263312	22.623715325475168
64-65	22.386058981233244	27.213826211041454	27.47786279700144	22.92225201072386
66-67	22.440602406847628	27.347647969751733	27.265032944016436	22.946716679384206
68-69	22.831674289237633	27.29941235916142	27.202248581391064	22.666664770209888
70-71	22.791472486352344	27.31969612129732	27.12275752708251	22.766073865267824
72-73	22.651613231513927	27.412385325957224	27.357061066030187	22.578940376498664
74-75	22.712860152556413	27.235473302966078	27.123115711895224	22.928550832582278
76-77	22.753613149306606	27.470817556630596	27.066506123754547	22.709063170308255
78-79	22.774420819264986	27.22783512977518	27.286541076388488	22.711202974571354
80-81	22.809882591644087	27.14597414844232	27.206355392247833	22.837787867665757
82-83	22.484365169815682	27.314030146561148	27.481167069086588	22.720437614536582
84-85	22.644084964348927	27.08023211646951	27.137250036751492	23.138432882430067
86-87	22.747558773940856	27.244661063938437	27.22893215546905	22.778848006651657
88-89	22.624721276023102	26.97588832873	27.424548241746848	22.974842153500045
90-91	22.7380568797848	26.990335051112968	27.263322097471494	23.008285971630738
92-93	22.588703931045732	27.190065435787737	27.370096755682432	22.851133877484102
94-95	22.513756773759795	26.91070989812697	27.34605981159025	23.229473516522976
96-97	22.6580428877958	26.97770686555094	27.333366861750296	23.030883384902964
98-99	22.57651534689598	26.95012364158605	27.370892731020973	23.102468280496993
100	22.801415105942564	26.628596691099787	27.259760359309404	23.31022784364825
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	357.0
1	431.5
2	409.0
3	324.5
4	365.5
5	487.0
6	719.5
7	1021.0
8	1414.5
9	2072.5
10	2889.5
11	3709.0
12	4715.0
13	5731.5
14	6733.0
15	7688.0
16	8550.0
17	9413.0
18	10324.5
19	11314.5
20	12272.5
21	13219.5
22	14324.5
23	15684.5
24	17255.5
25	19270.5
26	22263.5
27	26290.0
28	31655.5
29	38243.5
30	45354.5
31	53105.0
32	61051.5
33	69070.0
34	76751.0
35	84174.5
36	91348.0
37	97604.0
38	102397.5
39	106062.0
40	108520.5
41	109009.5
42	108367.0
43	106734.5
44	104201.0
45	101165.0
46	98101.0
47	95224.5
48	92298.5
49	89935.0
50	88570.5
51	85095.0
52	78850.0
53	73287.0
54	68897.5
55	64479.0
56	60019.5
57	54944.5
58	49736.5
59	45146.0
60	40142.5
61	34770.0
62	29659.0
63	24737.0
64	20198.5
65	16454.5
66	13177.0
67	10290.5
68	8042.0
69	6296.5
70	4814.0
71	3601.0
72	2696.5
73	2026.0
74	1472.5
75	1057.0
76	729.0
77	504.5
78	340.5
79	225.5
80	162.0
81	113.5
82	73.0
83	48.5
84	34.5
85	22.5
86	11.0
87	9.0
88	9.5
89	7.0
90	4.5
91	3.5
92	2.5
93	2.0
94	1.5
95	1.5
96	1.0
97	1.0
98	1.5
99	1.0
100	1.5
>>END_MODULE
>>Per base N content	fail
#Base	N-Count
1	8.44159722543787
2	25.774255440373828
3	6.6551171759918235
4	25.06866947317277
5	13.381068096360735
6	10.768775975448776
7	8.691344056038854
8	9.282005544560834
9	5.836016022988922
10-11	7.317266723441036
12-13	10.810484333696394
14-15	8.161298938534028
16-17	8.559256403390625
18-19	7.015241838013192
20-21	8.734931471456115
22-23	6.092054339648978
24-25	5.711293603353849
26-27	10.357883215254724
28-29	6.760696700711157
30-31	4.505291223662162
32-33	9.38246799395212
34-35	7.79233264143764
36-37	7.627143383470606
38-39	6.4684529789431515
40-41	9.29338055135564
42-43	6.981217481405723
44-45	7.0046050322493185
46-47	6.263098873974991
48-49	4.238582546645916
50-51	7.108674600314474
52-53	4.960291495454359
54-55	5.526038699182409
56-57	5.278858794893528
58-59	3.8438127680592493
60-61	4.465696804730392
62-63	3.2824612159244726
64-65	4.954536882872327
66-67	2.9490124547935754
68-69	5.635695106868021
70-71	2.831722377356287
72-73	1.7457447743755659
74-75	0.9108897402807445
76-77	1.1814907500055365
78-79	1.8327518322485132
80-81	2.36137087957995
82-83	0.8240169007770572
84-85	1.2804600200388025
86-87	0.4812231856864162
88-89	0.6360272973319402
90-91	0.2348821462054118
92-93	0.332240795807555
94-95	0.5809642113429286
96-97	0.5891179772754879
98-99	0.46919386434146765
100	0.4202712687461118
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2980218.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	93.97446708154075
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.48377196689982	90.67011052606311
2	2.57166111392296	4.833409653904633
3	0.49762922754060673	1.402933243869819
4	0.1714567318329997	0.6445022000619516
5	0.08274660888288868	0.38880342362870746
6	0.0523018583335048	0.2949023558559213
7	0.03740065212689119	0.2460294446488685
8	0.022279777433196323	0.16749841687839698
9	0.015166411481310732	0.12827298928459835
>10	0.06392306673798215	0.9188782288675645
>50	7.231641028977833E-4	0.04595630473119307
>100	8.676675920405102E-4	0.17772025603833774
>500	0.0	0.0
>1k	7.175311290607377E-5	0.08098295616694677
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	3.355459231505883E-5	0.0
6	0.0	0.0	0.0	3.355459231505883E-5	0.0
7	0.0	0.0	0.0	1.342183692602353E-4	0.0
8	0.0	0.0	0.0	1.342183692602353E-4	0.0
9	0.0	0.0	0.0	1.342183692602353E-4	0.0
10-11	0.0	0.0	0.0	1.342183692602353E-4	0.0
12-13	0.0	0.0	0.0	1.342183692602353E-4	0.0
14-15	0.0	0.0	0.0	1.5099566541776474E-4	0.0
16-17	0.0	0.0	0.0	2.01327553890353E-4	0.0
18-19	0.0	0.0	0.0	2.3488214620541182E-4	0.0
20-21	0.0	0.0	0.0	2.3488214620541182E-4	0.0
22-23	0.0	0.0	0.0	2.3488214620541182E-4	0.0
24-25	0.0	0.0	0.0	2.8521403467800005E-4	0.0
26-27	0.0	0.0	0.0	3.5232321930811776E-4	0.0
28-29	0.0	0.0	0.0	7.549783270888238E-4	0.0
30-31	0.0	0.0	0.0	0.0022481576851089416	0.0
32-33	0.0	0.0	0.0	0.006425704428333767	0.0
34-35	1.6777296157529414E-5	1.6777296157529414E-5	0.0	0.013052736410557885	0.0
36-37	3.355459231505883E-5	3.355459231505883E-5	0.0	0.02321977788202071	0.0
38-39	3.355459231505883E-5	5.033188847258824E-5	0.0	0.040030628631865184	0.0
40-41	3.355459231505883E-5	6.710918463011766E-5	0.0	0.0612539082711399	0.0
42-43	3.355459231505883E-5	6.710918463011766E-5	0.0	0.08219197387573661	0.0
44-45	3.355459231505883E-5	6.710918463011766E-5	0.0	0.10799545536601685	0.0
46-47	3.355459231505883E-5	6.710918463011766E-5	0.0	0.13886568029587099	0.0
48-49	6.710918463011766E-5	6.710918463011766E-5	0.0	0.17233638613014216	0.0
50-51	6.710918463011766E-5	6.710918463011766E-5	0.0	0.20533732767200252	0.0
52-53	6.710918463011766E-5	6.710918463011766E-5	0.0	0.24273392080713557	0.0
54-55	6.710918463011766E-5	6.710918463011766E-5	0.0	0.28640522270518465	0.0
56-57	6.710918463011766E-5	6.710918463011766E-5	0.0	0.3378276354280123	0.0
58-59	6.710918463011766E-5	6.710918463011766E-5	0.0	0.39653139468320775	0.0
60-61	6.710918463011766E-5	6.710918463011766E-5	0.0	0.4560907960424372	0.0
62-63	6.710918463011766E-5	6.710918463011766E-5	0.0	0.5207840500258706	0.0
64-65	6.710918463011766E-5	6.710918463011766E-5	0.0	0.5960973324770201	0.0
66-67	6.710918463011766E-5	6.710918463011766E-5	0.0	0.6848324518541933	0.0
68-69	6.710918463011766E-5	6.710918463011766E-5	0.0	0.7844895910299179	0.0
70-71	6.710918463011766E-5	8.388648078764708E-5	0.0	0.8984745411241728	0.0
72-73	6.710918463011766E-5	1.006637769451765E-4	0.0	1.022643309986048	0.0
74-75	6.710918463011766E-5	1.006637769451765E-4	0.0	1.156794570061653	0.0
76-77	6.710918463011766E-5	1.1744107310270591E-4	0.0	1.3067835977099662	0.0
78-79	6.710918463011766E-5	1.342183692602353E-4	0.0	1.4747411095429932	0.0
80-81	6.710918463011766E-5	1.342183692602353E-4	0.0	1.6692235266010742	0.0
82-83	6.710918463011766E-5	1.342183692602353E-4	0.0	1.8800638074127463	0.0
84-85	8.388648078764708E-5	1.342183692602353E-4	0.0	2.0955681765562115	0.0
86-87	1.006637769451765E-4	1.342183692602353E-4	0.0	2.3283699380380902	0.0
88	1.006637769451765E-4	1.342183692602353E-4	0.0	2.525687718146793	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATAGG	560	0.0	20.086323	1
GTACATG	8315	0.0	19.915884	1
TACATGG	8605	0.0	19.311977	2
GTATCAA	5060	0.0	18.895433	1
CATGGGG	4640	0.0	17.902763	4
ACATGGG	8870	0.0	17.772642	3
TACGTCG	175	0.0014767074	17.251257	5
GTACGGT	235	2.512283E-4	16.832588	4
TCAACGC	5555	0.0	16.47979	4
ATATCGT	245	4.4772292E-5	16.432428	6
GTATAGA	1040	0.0	16.223568	1
CAACGCA	5620	0.0	16.20503	5
TATCAAC	5750	0.0	16.188791	2
ATCAACG	5595	0.0	16.158138	3
TATATAC	1500	0.0	15.820837	3
TCTATAC	960	0.0	15.302893	3
AACGCAG	5700	0.0	15.27387	6
GTGTATA	1235	0.0	14.673948	1
GTATTAC	925	0.0	14.187097	1
AGTACGG	360	2.7903181E-5	14.125748	3
>>END_MODULE