FastQCFastQC Report
Wed 25 May 2016
SRR1295129_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295129_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2980218
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT41200.1382449203380424No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA75850.025.8763181
TATCAAC87000.022.3705962
CAACGCA84700.022.2058945
TCAACGC85400.022.1339974
ATCAACG86100.021.953313
AACGCAG86700.021.5518466
TAGACCG1857.1587074E-620.3333975
CGCAGAG98000.018.9214788
ACGCAGA98300.018.9114887
GTACATG105900.017.0245931
GGTATCA55050.016.6926771
GCAGAGT111100.016.3946349
ACATGGG108800.016.033273
TACATGG112050.015.8996862
GTATATA15550.015.7373351
GTATAAG9500.014.7197321
GAGTACT33900.014.4018312-13
CATGGGG55800.014.241124
AGAGTAC100050.014.099750510-11
GTATTAG12850.013.991521
Produced by FastQC (version 0.11.5)