##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295129_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2980218 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.08704765892965 33.0 33.0 33.0 27.0 33.0 2 29.730668024956564 33.0 33.0 33.0 22.0 33.0 3 30.646905025068637 33.0 33.0 33.0 27.0 33.0 4 34.920834985897 37.0 37.0 37.0 33.0 37.0 5 35.23927477788538 37.0 37.0 37.0 33.0 37.0 6 34.961636027968424 37.0 37.0 37.0 33.0 37.0 7 34.84270445987508 37.0 37.0 37.0 33.0 37.0 8 34.84150186328651 37.0 37.0 37.0 33.0 37.0 9 34.809987054638285 37.0 37.0 37.0 27.0 37.0 10-11 34.77521057184407 37.0 37.0 37.0 27.0 37.0 12-13 34.663412038985065 37.0 37.0 37.0 27.0 37.0 14-15 36.05036611415675 37.0 37.0 40.0 27.0 40.0 16-17 36.03670956285748 37.0 37.0 40.0 27.0 40.0 18-19 36.05396467640958 37.0 37.0 40.0 27.0 40.0 20-21 36.04616205928559 37.0 37.0 40.0 27.0 40.0 22-23 36.0020110944904 37.0 37.0 40.0 27.0 40.0 24-25 35.96380147358347 37.0 37.0 40.0 27.0 40.0 26-27 35.94800950802928 37.0 37.0 40.0 27.0 40.0 28-29 35.889964760967146 37.0 37.0 40.0 27.0 40.0 30-31 35.804793474839755 37.0 37.0 40.0 27.0 40.0 32-33 35.76084299873365 37.0 37.0 40.0 27.0 40.0 34-35 35.64129352282282 37.0 37.0 40.0 27.0 40.0 36-37 35.63502669938911 37.0 37.0 40.0 27.0 40.0 38-39 35.56779470495111 37.0 37.0 40.0 27.0 40.0 40-41 35.50687399378167 37.0 37.0 40.0 27.0 40.0 42-43 35.40907091360431 37.0 35.0 40.0 27.0 40.0 44-45 35.300706525495784 37.0 33.0 40.0 27.0 40.0 46-47 35.193027993254184 37.0 33.0 40.0 27.0 40.0 48-49 35.090129648233784 37.0 33.0 40.0 27.0 40.0 50-51 34.98114970112925 37.0 33.0 40.0 27.0 40.0 52-53 34.87508246041062 37.0 33.0 40.0 24.5 40.0 54-55 34.73821696936264 37.0 33.0 40.0 22.0 40.0 56-57 34.60326912326548 37.0 33.0 40.0 22.0 40.0 58-59 34.46322701896304 37.0 33.0 40.0 22.0 40.0 60-61 34.294157675713656 37.0 33.0 40.0 22.0 40.0 62-63 34.10265423536131 37.0 33.0 40.0 22.0 40.0 64-65 33.915499973491876 37.0 33.0 40.0 22.0 40.0 66-67 33.68541512734974 37.0 33.0 37.0 22.0 40.0 68-69 33.461366920138055 37.0 33.0 37.0 22.0 40.0 70-71 33.240553207852585 37.0 33.0 37.0 22.0 40.0 72-73 33.02775501657932 37.0 33.0 37.0 22.0 40.0 74-75 32.79608068939923 37.0 33.0 37.0 22.0 38.5 76-77 32.57505759645771 37.0 33.0 37.0 22.0 37.0 78-79 32.354251266182544 37.0 33.0 37.0 22.0 37.0 80-81 32.154201806713466 35.0 33.0 37.0 22.0 37.0 82-83 31.95805441078471 33.0 33.0 37.0 22.0 37.0 84-85 31.79345638473427 33.0 33.0 37.0 22.0 37.0 86-87 31.83608397103836 33.0 33.0 37.0 22.0 37.0 88-89 31.86027280554644 33.0 33.0 37.0 22.0 37.0 90-91 31.88284716084528 33.0 33.0 37.0 22.0 37.0 92-93 31.869521457826238 33.0 33.0 37.0 22.0 37.0 94-95 31.90429374629641 33.0 33.0 37.0 22.0 37.0 96-97 31.869659534973614 33.0 33.0 37.0 22.0 37.0 98-99 31.800546637863405 33.0 33.0 37.0 22.0 37.0 100 31.25761706022848 33.0 27.0 37.0 22.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6481927679449839 1101 2 4.10924749022109 1101 3 2.3754322781071053 1101 4 1.2372923086308987 1101 5 0.5932542516677657 1101 6 0.6409443256716898 1101 7 0.6379152203781828 1101 8 0.648043459215728 1101 9 0.7159185357535165 1101 10-11 0.6719625163657881 1101 12-13 0.7846699683512739 1101 14-15 1.0802822507086205 1101 16-17 1.1163742531505534 1101 18-19 1.1435891864363796 1101 20-21 1.1066291731541256 1101 22-23 1.0406154573510165 1101 24-25 1.028771979756847 1101 26-27 1.082280260708579 1101 28-29 0.9164426523319094 1101 30-31 0.8533981792034808 1101 32-33 1.0688483518438403 1101 34-35 1.2072854066731509 1101 36-37 1.0412542490607564 1101 38-39 1.054252288291373 1101 40-41 1.1226859719260176 1101 42-43 1.1602162226787058 1101 44-45 1.1089043283099471 1101 46-47 1.115932839638205 1101 48-49 1.1185871045181344 1101 50-51 1.0811812603932367 1101 52-53 1.1716089686610474 1101 54-55 1.0969200370349483 1101 56-57 1.0080835756230542 1101 58-59 1.0498940917029032 1101 60-61 0.98970878782292 1101 62-63 0.9864582129297403 1101 64-65 1.047260902735701 1101 66-67 0.9532867718788367 1101 68-69 1.0295760015863422 1101 70-71 0.9862322681925306 1101 72-73 0.9087005024529873 1101 74-75 0.9049885258859334 1101 76-77 0.9624525620268827 1101 78-79 1.0148047050389337 1101 80-81 0.9441041055416761 1101 82-83 0.9406859395542924 1101 84-85 0.9897346544064618 1101 86-87 0.9446932747554264 1101 88-89 0.9790170184997677 1101 90-91 0.905467502430696 1101 92-93 0.8926660481710194 1101 94-95 0.9692332186854067 1101 96-97 0.953813274706647 1101 98-99 0.9142445437616331 1101 100 1.135475634636876 1102 1 0.4728343849389809 1102 2 3.9876604693289686 1102 3 2.2536806947243804 1102 4 1.1326722241199079 1102 5 0.5062993327960896 1102 6 0.5598828689447615 1102 7 0.563711176529246 1102 8 0.5566322572839155 1102 9 0.5793010292893683 1102 10-11 0.6693397707854345 1102 12-13 0.6757098323050386 1102 14-15 0.9572964486463604 1102 16-17 0.8660571367835246 1102 18-19 0.8843325710489722 1102 20-21 0.904775562015594 1102 22-23 0.8953621512016312 1102 24-25 0.8913417174363261 1102 26-27 0.8859429769859446 1102 28-29 0.9121899474039168 1102 30-31 0.8862395892934956 1102 32-33 1.0046244053229927 1102 34-35 1.125511216456502 1102 36-37 0.9753879392327818 1102 38-39 0.9877929604792328 1102 40-41 1.0181392239723976 1102 42-43 0.9958403103576003 1102 44-45 1.0975763505220257 1102 46-47 1.1088455581640986 1102 48-49 0.9552961927262942 1102 50-51 1.0072140778108079 1102 52-53 1.0135671802650847 1102 54-55 1.0042448666880617 1102 56-57 0.9812592966365514 1102 58-59 0.9339605336355206 1102 60-61 0.8720431230601378 1102 62-63 1.060960050643061 1102 64-65 0.984207944351823 1102 66-67 0.8833921155935442 1102 68-69 0.9241700199918412 1102 70-71 0.8590167653582412 1102 72-73 0.9036404529577311 1102 74-75 0.8970393668991008 1102 76-77 0.89442383682875 1102 78-79 0.9504404850557577 1102 80-81 0.864542836060167 1102 82-83 0.9538252907748301 1102 84-85 0.9714650304777166 1102 86-87 0.9905223222983714 1102 88-89 0.9063630989523404 1102 90-91 0.9254677752189622 1102 92-93 0.9798508109297295 1102 94-95 0.9727135671071885 1102 96-97 0.9906059636251285 1102 98-99 1.0121378523147158 1102 100 0.9288228030797754 1103 1 0.5232516478757532 1103 2 3.9362761360877023 1103 3 2.254188457837664 1103 4 1.1636887599281067 1103 5 0.597829297563635 1103 6 0.5689306636183318 1103 7 0.48860607088859354 1103 8 0.5442616601975487 1103 9 0.5324190754586695 1103 10-11 0.5819543983894349 1103 12-13 0.6053475498552672 1103 14-15 0.8722426927953677 1103 16-17 0.8572887879120685 1103 18-19 0.9086432443450079 1103 20-21 0.8571522075999312 1103 22-23 0.8180162713812109 1103 24-25 0.8731469433126122 1103 26-27 0.8178784169130182 1103 28-29 0.7918991324437457 1103 30-31 0.8267864333568014 1103 32-33 0.9171218020660419 1103 34-35 0.9787474076551774 1103 36-37 0.9154345383439022 1103 38-39 0.8974410241181445 1103 40-41 0.774448984298914 1103 42-43 0.8377628430257644 1103 44-45 0.949542959096533 1103 46-47 0.8596143088700217 1103 48-49 0.8205162952600276 1103 50-51 0.8008230838784343 1103 52-53 0.8342145908480489 1103 54-55 0.7692852601941098 1103 56-57 0.7684967860543068 1103 58-59 0.7149903698450117 1103 60-61 0.7663470819639144 1103 62-63 0.843421584508782 1103 64-65 0.8455026051636167 1103 66-67 0.8719958946659077 1103 68-69 0.7579052127737285 1103 70-71 0.7851042998325113 1103 72-73 0.7718995285895787 1103 74-75 0.7885034965535525 1103 76-77 0.7177159541542295 1103 78-79 0.726680216084425 1103 80-81 0.7511594377684219 1103 82-83 0.8370561598148072 1103 84-85 0.8172368366542315 1103 86-87 0.7959043690195706 1103 88-89 0.750196755666515 1103 90-91 0.7395484842020394 1103 92-93 0.783424003075492 1103 94-95 0.7531548886590436 1103 96-97 0.799037049288053 1103 98-99 0.829204445188509 1103 100 0.8669853535157728 1104 1 0.5256469285374692 1104 2 3.691082525173613 1104 3 2.163405354107109 1104 4 1.1010690636562543 1104 5 0.46368737799126336 1104 6 0.4033647040561661 1104 7 0.4825191836710374 1104 8 0.4020625963892144 1104 9 0.42254971986506007 1104 10-11 0.5315787213097707 1104 12-13 0.493596197032268 1104 14-15 0.7181053484611937 1104 16-17 0.6550041465247958 1104 18-19 0.5915704376713649 1104 20-21 0.5982747868946277 1104 22-23 0.5438936078482755 1104 24-25 0.6857261026109498 1104 26-27 0.6577311495109655 1104 28-29 0.6285208835638514 1104 30-31 0.5784088010725696 1104 32-33 0.7503775787329019 1104 34-35 0.8943006911399465 1104 36-37 0.7443611351697328 1104 38-39 0.659033104644557 1104 40-41 0.7268487693489476 1104 42-43 0.7435400154110638 1104 44-45 0.8057103895173512 1104 46-47 0.7426591583824802 1104 48-49 0.5765168704299484 1104 50-51 0.553940724457398 1104 52-53 0.5641593281932984 1104 54-55 0.6223147847008903 1104 56-57 0.5715832715958982 1104 58-59 0.6561104504837729 1104 60-61 0.6702334954352978 1104 62-63 0.6850812285907466 1104 64-65 0.6808339807710269 1104 66-67 0.5559271013984741 1104 68-69 0.5976666347514978 1104 70-71 0.5816264052600033 1104 72-73 0.6201778878364479 1104 74-75 0.6279197207677569 1104 76-77 0.611499010760781 1104 78-79 0.6283446411465903 1104 80-81 0.5253503794069516 1104 82-83 0.6625608839007882 1104 84-85 0.6041671667392876 1104 86-87 0.6399351014285735 1104 88-89 0.5594724825503548 1104 90-91 0.6183494762919892 1104 92-93 0.6987457007404991 1104 94-95 0.6445735637244177 1104 96-97 0.6280163801086616 1104 98-99 0.6022817326002254 1104 100 0.585033841207423 1105 1 0.4663602997180867 1105 2 3.7558756269580904 1105 3 2.144407304354477 1105 4 1.0860505675606262 1105 5 0.4614195709240576 1105 6 0.3571727533237521 1105 7 0.41544803917157225 1105 8 0.41297753981116614 1105 9 0.46184457539538926 1105 10-11 0.4233173059208326 1105 12-13 0.4400260883829077 1105 14-15 0.5953736424855265 1105 16-17 0.5335622085551108 1105 18-19 0.6143533600786597 1105 20-21 0.5840561990346416 1105 22-23 0.48210051841277135 1105 24-25 0.5077537004126285 1105 26-27 0.515377312078769 1105 28-29 0.5626646748095965 1105 30-31 0.5552460139158626 1105 32-33 0.6074737534650865 1105 34-35 0.6827342818370141 1105 36-37 0.5560152094858779 1105 38-39 0.46808833016554274 1105 40-41 0.4740362004827148 1105 42-43 0.5421353127929152 1105 44-45 0.5640311500208881 1105 46-47 0.6561162980714386 1105 48-49 0.5536965573540655 1105 50-51 0.5403815974868564 1105 52-53 0.5014925803883301 1105 54-55 0.5326098985412813 1105 56-57 0.5712623763993818 1105 58-59 0.6358600233937324 1105 60-61 0.5362795474479185 1105 62-63 0.4914905391600328 1105 64-65 0.5302042274488628 1105 66-67 0.5083388377144402 1105 68-69 0.4817603489816378 1105 70-71 0.496766888889276 1105 72-73 0.47299851536567417 1105 74-75 0.4998190483340821 1105 76-77 0.4893094631140329 1105 78-79 0.5193526754935576 1105 80-81 0.47120762191071464 1105 82-83 0.5208704412077481 1105 84-85 0.4901421777546986 1105 86-87 0.5431335122042178 1105 88-89 0.5204279293471998 1105 90-91 0.4402768564079196 1105 92-93 0.5072159666292713 1105 94-95 0.5553853231597081 1105 96-97 0.44968408795119075 1105 98-99 0.5482338203477397 1105 100 0.5189664521976809 1106 1 0.47158475737023053 1106 2 3.800671708470915 1106 3 2.1140676909513765 1106 4 1.015706142911327 1106 5 0.39619684552079804 1106 6 0.330441149018462 1106 7 0.18458467080358787 1106 8 0.16408626671587 1106 9 0.22367765018473307 1106 10-11 0.1593937671866641 1106 12-13 0.22890344841556498 1106 14-15 0.23729409070742946 1106 16-17 0.2445458180261042 1106 18-19 0.2864451031841426 1106 20-21 0.28065867569997494 1106 22-23 0.25577999779688554 1106 24-25 0.2974219372010438 1106 26-27 0.16483893813479256 1106 28-29 0.1878258528089347 1106 30-31 0.1870074607141703 1106 32-33 0.31765838816708225 1106 34-35 0.3657401567140752 1106 36-37 0.25559930704052647 1106 38-39 0.21394334894367262 1106 40-41 0.19644942344942962 1106 42-43 0.25807909931376116 1106 44-45 0.29386891189524533 1106 46-47 0.2716080978004385 1106 48-49 0.06924439233083035 1106 50-51 0.07471603466992605 1106 52-53 0.1981958754411295 1106 54-55 0.18523667124895127 1106 56-57 0.14496711357441683 1106 58-59 0.10527843334444498 1106 60-61 0.21448045928170956 1106 62-63 0.24185655183598698 1106 64-65 0.16152505220961189 1106 66-67 0.10894618870786843 1106 68-69 0.12090844800118816 1106 70-71 0.11418656648571357 1106 72-73 0.09506118322422452 1106 74-75 0.051235787615844686 1106 76-77 0.13380608084800372 1106 78-79 0.1880175696924482 1106 80-81 0.12836615297366194 1106 82-83 0.09402580265574656 1106 84-85 0.10081229273146874 1106 86-87 0.1991578332790489 1106 88-89 0.19014662914922198 1106 90-91 0.20106332243744163 1106 92-93 0.20420970124523663 1106 94-95 0.2321369409475018 1106 96-97 0.18673759266368606 1106 98-99 0.24100865277393524 1106 100 0.1873686052929351 1107 1 0.41190170378750324 1107 2 3.4688772348319823 1107 3 2.050098741541934 1107 4 0.9630795396373983 1107 5 0.2848306261041529 1107 6 0.10199245751608998 1107 7 0.10551689108540785 1107 8 0.1663066112295084 1107 9 0.10892852015635412 1107 10-11 0.15600491064518707 1107 12-13 0.10506727087120282 1107 14-15 0.17085009206120816 1107 16-17 0.04645923314822653 1107 18-19 0.11716010447135261 1107 20-21 0.18582082244829934 1107 22-23 0.09212308281990289 1107 24-25 0.07001851029551887 1107 26-27 0.08495313282553241 1107 28-29 0.0453820995629286 1107 30-31 -0.009836399966573595 1107 32-33 0.13807250152546402 1107 34-35 0.2643444405101718 1107 36-37 0.06094461827935049 1107 38-39 0.06980871259425214 1107 40-41 0.042617025426601174 1107 42-43 0.092915699690856 1107 44-45 0.1741479397004042 1107 46-47 0.08961099981221565 1107 48-49 0.013290581109274058 1107 50-51 0.06752755486653683 1107 52-53 0.049818407718575486 1107 54-55 0.10430888528729554 1107 56-57 0.05675145873251353 1107 58-59 0.10676011425829302 1107 60-61 0.15639432320958235 1107 62-63 0.17679947240304728 1107 64-65 0.05887494385437009 1107 66-67 0.05272028278451302 1107 68-69 -0.07928331942866862 1107 70-71 0.020345415258177013 1107 72-73 0.0782839587523938 1107 74-75 0.050816263710181886 1107 76-77 0.07840548987732632 1107 78-79 2.3100200055381492E-4 1107 80-81 0.13586302501655467 1107 82-83 0.13892307042389973 1107 84-85 0.137020492223332 1107 86-87 0.04203017113205121 1107 88-89 0.0892716420486579 1107 90-91 0.09555334626195844 1107 92-93 0.03800302729484528 1107 94-95 0.029466890093132747 1107 96-97 -0.006830334384183345 1107 98-99 0.09428350668567731 1107 100 0.12452042632472882 1108 1 0.4458326768404959 1108 2 3.603519496086257 1108 3 2.0992999792588556 1108 4 1.000272634031674 1108 5 0.21725616768451772 1108 6 0.08715443927707867 1108 7 0.07449386563371263 1108 8 0.04949918104663453 1108 9 0.05259831062154774 1108 10-11 0.07502667921081496 1108 12-13 0.008590225280215691 1108 14-15 0.012712210253852163 1108 16-17 0.015970686267536394 1108 18-19 0.0730558199592437 1108 20-21 0.02883593607253232 1108 22-23 -0.037267812607950646 1108 24-25 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1.1139949166668686 2202 92-93 1.1622401816723418 2202 94-95 1.1290215808594404 2202 96-97 1.1050840492538825 2202 98-99 1.0087178143582989 2202 100 0.8962349846234829 2203 1 0.4375769461857999 2203 2 3.9583108918174865 2203 3 2.220774530488228 2203 4 1.0850948580752444 2203 5 0.5523484144763842 2203 6 0.6114507566343832 2203 7 0.5915279890528566 2203 8 0.5869743541128827 2203 9 0.5993280942140942 2203 10-11 0.6781824771505853 2203 12-13 0.7408424159133489 2203 14-15 1.0029587521324572 2203 16-17 1.048986573329202 2203 18-19 1.1249688024479596 2203 20-21 1.0952720371360058 2203 22-23 1.0904600675158278 2203 24-25 0.9554068965523115 2203 26-27 0.9801568515916586 2203 28-29 0.9859970670901888 2203 30-31 0.9723640899059731 2203 32-33 1.034685936770721 2203 34-35 1.2046944722440358 2203 36-37 1.0639204199581442 2203 38-39 1.0005455250240871 2203 40-41 0.5769948213117431 2203 42-43 0.7851049445514207 2203 44-45 0.9588323745734186 2203 46-47 1.0567530090492454 2203 48-49 1.064627201358725 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2210 82-83 0.10762333694611215 2210 84-85 0.15341213171650736 2210 86-87 0.22529335470315104 2210 88-89 0.2788739238738174 2210 90-91 0.30472836949473603 2210 92-93 0.31698946474144307 2210 94-95 0.13797957346744383 2210 96-97 0.16061663428112638 2210 98-99 0.23534628830357462 2210 100 0.039042040662160105 2211 1 0.6463024520702056 2211 2 4.07809280612523 2211 3 2.3470258631302165 2211 4 1.172397923203775 2211 5 0.6177238298554357 2211 6 0.48137970317699086 2211 7 0.4857262944933609 2211 8 0.38582511275765086 2211 9 0.44577718780972475 2211 10-11 0.48195024311267076 2211 12-13 0.4721800247847483 2211 14-15 0.5311131910512188 2211 16-17 0.42802268610864047 2211 18-19 0.5542322696654836 2211 20-21 0.63876718559181 2211 22-23 0.5218796611457392 2211 24-25 0.5396942941752769 2211 26-27 0.5839027965153534 2211 28-29 0.5956582798827199 2211 30-31 0.5031673950659226 2211 32-33 0.5986532717153565 2211 34-35 0.6232902572506944 2211 36-37 0.5738109614731854 2211 38-39 0.5395722443660418 2211 40-41 0.4466528752491854 2211 42-43 0.5796390861637306 2211 44-45 0.580119732710699 2211 46-47 0.5734788349755462 2211 48-49 0.5350899320642455 2211 50-51 0.4403964768781492 2211 52-53 0.3963290034254783 2211 54-55 0.43764844579887097 2211 56-57 0.4700799464984442 2211 58-59 0.5113155575638828 2211 60-61 0.5263233078447627 2211 62-63 0.6782316099195711 2211 64-65 0.6261312349356629 2211 66-67 0.619872399840709 2211 68-69 0.4992860239600674 2211 70-71 0.4821638903172669 2211 72-73 0.509669149870021 2211 74-75 0.5354103382167352 2211 76-77 0.4219067839672306 2211 78-79 0.3469987986092775 2211 80-81 0.5504450905729996 2211 82-83 0.20309631250850657 2211 84-85 0.5205663581571471 2211 86-87 0.6780421698690269 2211 88-89 0.6808574192302856 2211 90-91 0.604044039963938 2211 92-93 0.7187851750670653 2211 94-95 0.6843583882500859 2211 96-97 0.6010641247321971 2211 98-99 0.6575124588941392 2211 100 0.49824363491874735 2212 1 0.6272379178204623 2212 2 4.140677244453979 2212 3 2.4270266656188753 2212 4 1.1455036959295413 2212 5 0.5631210853442212 2212 6 0.6213898995034839 2212 7 0.46808523350894404 2212 8 0.3845545843896758 2212 9 0.3250765208000317 2212 10-11 0.35634719648808044 2212 12-13 0.45599583475543426 2212 14-15 0.5722970270414152 2212 16-17 0.5493509464548438 2212 18-19 0.5311801928720357 2212 20-21 0.5638448381708656 2212 22-23 0.5725131551341605 2212 24-25 0.4547712858813142 2212 26-27 0.4995018779018636 2212 28-29 0.35592517816964175 2212 30-31 0.40292204595975534 2212 32-33 0.5940434464218569 2212 34-35 0.649059820272015 2212 36-37 0.5358587945177788 2212 38-39 0.660346905550611 2212 40-41 0.4936309153830649 2212 42-43 0.5395705913964193 2212 44-45 0.6315099262992874 2212 46-47 0.6984837915231452 2212 48-49 0.5990068556059569 2212 50-51 0.6422401945720821 2212 52-53 0.5002777196796515 2212 54-55 0.42934882485675274 2212 56-57 0.5812330046883076 2212 58-59 0.5302177058732269 2212 60-61 0.4919091717710131 2212 62-63 0.6327424222741129 2212 64-65 0.681604443615754 2212 66-67 0.47862235853614976 2212 68-69 0.4675762746669605 2212 70-71 0.43491123384378483 2212 72-73 0.6113225125335333 2212 74-75 0.5158881494053134 2212 76-77 0.4833526325045767 2212 78-79 0.5011828328613817 2212 80-81 0.4433775257484385 2212 82-83 0.5719772184709981 2212 84-85 0.599615267480651 2212 86-87 0.4346095954382072 2212 88-89 0.713275364764911 2212 90-91 0.6012979945808468 2212 92-93 0.6080340692848942 2212 94-95 0.7239072716107238 2212 96-97 0.459261192344222 2212 98-99 0.5436049763954784 2212 100 0.4103529527561811 2213 1 -1.0330382718120354 2213 2 -25.650478622446197 2213 3 -14.667940522807465 2213 4 -6.937476535352868 2213 5 -2.739807083545088 2213 6 -1.8273211474003759 2213 7 -1.5804813470685133 2213 8 -1.4912837632426488 2213 9 -1.5825498701994434 2213 10-11 -1.4127821553896425 2213 12-13 -1.4093794369707382 2213 14-15 -1.773125536963299 2213 16-17 -1.8778049749609238 2213 18-19 -1.8775128298132628 2213 20-21 -1.7363447738825286 2213 22-23 -1.8994907018441793 2213 24-25 -1.8395950384728437 2213 26-27 -2.0226936354646767 2213 28-29 -1.8984081301773799 2213 30-31 -1.982410022157481 2213 32-33 -1.8265924098477768 2213 34-35 -1.7447008698954107 2213 36-37 -1.822416502421845 2213 38-39 -2.0555319539831416 2213 40-41 -1.828380948573269 2213 42-43 -1.916739929431941 2213 44-45 -1.8548509533098354 2213 46-47 -2.131766741707523 2213 48-49 -2.035346998305876 2213 50-51 -1.9689997285609309 2213 52-53 -2.1755783455210036 2213 54-55 -2.0658251821076163 2213 56-57 -2.03609801400372 2213 58-59 -1.7422042175732528 2213 60-61 -1.8379451012294652 2213 62-63 -1.7759819468782325 2213 64-65 -1.8835958671231339 2213 66-67 -1.8919402424028782 2213 68-69 -1.8231037733067588 2213 70-71 -1.7439224050040352 2213 72-73 -1.8775706164571844 2213 74-75 -1.8813138376881184 2213 76-77 -1.7744989760461891 2213 78-79 -1.9436909976087442 2213 80-81 -2.0881686046664925 2213 82-83 -2.0901436426028255 2213 84-85 -1.8733715738176357 2213 86-87 -1.8497788418041274 2213 88-89 -2.0133432373189493 2213 90-91 -1.8493100246631577 2213 92-93 -1.9232093292172863 2213 94-95 -1.9931191411949918 2213 96-97 -2.0177282861459673 2213 98-99 -1.975408870565623 2213 100 -1.9329770642117978 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 11.0 4 33.0 5 51.0 6 36.0 7 44.0 8 63.0 9 100.0 10 507.0 11 2464.0 12 7313.0 13 13309.0 14 17098.0 15 19139.0 16 20242.0 17 20521.0 18 21013.0 19 21816.0 20 23216.0 21 25668.0 22 28017.0 23 29628.0 24 31338.0 25 33569.0 26 36548.0 27 41357.0 28 48348.0 29 57297.0 30 68522.0 31 83633.0 32 104919.0 33 137632.0 34 186847.0 35 261627.0 36 394575.0 37 656832.0 38 584133.0 39 2749.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.22942306461559 19.968251098056225 10.163678186140805 31.638647651187384 2 12.991508152415879 22.020106609337255 35.66390776005704 29.32447747818982 3 15.24918646890932 26.22100128245652 28.42248452965521 30.10732771897895 4 10.830666166454032 18.058694002079147 36.11074385600856 34.99989597545826 5 11.97861476566546 38.13805427034214 34.51514714615406 15.368183817838348 6 30.80616168893825 38.58525704255852 17.371968894765008 13.236612373738222 7 26.958293780319316 32.55903413218114 22.488590605829096 17.994081481670452 8 25.13443589961359 36.24279061367357 20.269734525394863 18.353038961317978 9 25.289176355112392 16.63667875358596 21.054838270037575 37.01930662126407 10-11 23.758217315452598 27.122956853869674 28.37421443924533 20.744611391432407 12-13 24.48869225763603 24.48677883465491 28.675580644148813 22.348948263560253 14-15 21.98366738712603 26.043338677052652 25.740994160066187 26.23199977575513 16-17 21.61039179875381 29.362836533959104 26.540041360693646 22.48673030659344 18-19 21.615896591532895 28.448555808091786 28.42497668519823 21.510570915177084 20-21 22.695815259782538 27.75347273964823 27.74115520363874 21.809556796930483 22-23 22.175651821064722 27.576578392299727 27.876144138598708 22.371625648036847 24-25 22.174676714319013 27.516800484897058 27.748408637866508 22.560114162917426 26-27 21.972712454875644 27.589039744333636 27.798783886683125 22.639463914107594 28-29 21.860189682810965 27.540203013197655 28.10973136686637 22.48987593712501 30-31 22.357328398291195 27.866391386364565 27.567667620347454 22.208612594996794 32-33 21.697502409675952 27.74336049339426 28.09580653468885 22.463330562240944 34-35 21.835105834127347 27.44669117246116 28.094234058020984 22.623968935390504 36-37 22.276402112283073 27.69854833389428 27.641922584295187 22.38312696952746 38-39 22.056120240400265 27.783747143485133 27.82505545246189 22.335077163652713 40-41 22.121253355476387 27.580350645709512 27.70944389066302 22.588952108151076 42-43 21.968293160912058 27.606101098393687 27.84727656621436 22.578329174479897 44-45 22.183841876180907 27.408796435264332 27.801602600832858 22.6057590877219 46-47 22.244027169168987 27.661483484416728 27.71732801378393 22.377161332630354 48-49 22.14206001453291 27.63665716265949 27.662987569832637 22.558295252974965 50-51 22.211978334344952 27.58541587341329 27.485619074932178 22.716986717309585 52-53 22.022633884705975 27.609311661057284 27.505313601056596 22.862740853180146 54-55 22.26337399527536 27.688471717684145 27.3578273832848 22.690326903755697 56-57 22.232327481549184 27.52464009941785 27.429430657943556 22.81360176108941 58-59 22.02556870475645 27.22936677612122 27.846242107836648 22.898822411285682 60-61 22.296627483512605 27.59853509503157 27.347281005902307 22.757556415553523 62-63 21.91248537585807 27.73900194048855 27.566750859661266 22.781761823992117 64-65 21.963782142446615 27.324555331532764 27.610901771458153 23.100760754562472 66-67 22.06738302078633 27.475664762650155 27.490213800374786 22.966738416188733 68-69 22.213613016715954 27.45275929231995 27.46638633885229 22.867241352111805 70-71 22.24012867134651 27.37686295457753 27.330393453523033 23.052614920552926 72-73 22.04970174055439 27.518977476738183 27.566402289279274 22.864918493428156 74-75 22.26018586934505 27.32619031276992 27.3365948879637 23.077028929921326 76-77 22.113687357501494 27.51565501362331 27.394969151438435 22.975688477436762 78-79 22.16049850894332 27.49521271133371 27.471200758717295 22.873088021005678 80-81 22.120722155607037 27.349276008699906 27.38840613853073 23.141595697162327 82-83 21.844183139099318 27.44297855654459 27.56774560408409 23.145092700272002 84-85 21.961765078480838 27.23616066821763 27.403097620951268 23.398976632350262 86-87 22.05384931473981 27.420458189153322 27.36052382500038 23.165168671106485 88-89 21.839644374526216 27.20449639243635 27.578642068813004 23.377217164224433 90-91 21.932517087340067 27.160749856375066 27.538805577479607 23.367927478805257 92-93 21.699410973275768 27.467869629914983 27.506643685223125 23.326075711586128 94-95 21.660630376896346 27.090908755357706 27.668979901982084 23.57948096576386 96-97 21.822107441933582 27.19969035494926 27.578626371201935 23.399575831915218 98-99 21.67883532531574 27.191574919376134 27.566365579188222 23.563224176119903 100 21.79309399614631 26.94229678778644 27.502546283232693 23.762062932834557 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 21.0 1 18.0 2 16.5 3 13.5 4 17.5 5 28.5 6 59.0 7 135.0 8 222.0 9 243.0 10 204.5 11 195.0 12 233.5 13 265.5 14 289.5 15 333.5 16 394.0 17 489.5 18 594.5 19 735.0 20 945.0 21 1252.5 22 1716.5 23 2534.0 24 3772.0 25 5546.5 26 8384.0 27 12627.5 28 18730.5 29 26297.5 30 34593.0 31 44074.0 32 53722.5 33 63484.5 34 73521.5 35 82404.5 36 91132.0 37 99773.5 38 106989.5 39 112191.5 40 115942.5 41 118040.5 42 117838.0 43 116635.0 44 114411.0 45 111320.5 46 108389.5 47 105022.0 48 101936.0 49 100473.0 50 103677.5 51 100912.5 52 89856.5 53 83017.0 54 78652.0 55 74102.0 56 69340.0 57 63814.0 58 57787.0 59 52720.0 60 47515.5 61 41030.5 62 34616.0 63 28500.5 64 22811.5 65 18396.5 66 14539.0 67 11010.0 68 8357.0 69 6296.0 70 4654.0 71 3341.0 72 2437.5 73 1751.0 74 1216.5 75 775.0 76 410.5 77 212.0 78 114.5 79 62.0 80 31.5 81 13.5 82 12.5 83 11.5 84 8.0 85 5.5 86 3.5 87 2.0 88 1.0 89 2.0 90 1.5 91 1.0 92 1.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.907483949160766 2 5.790985760102113 3 0.0 4 0.0051002980318889425 5 0.15794146602698192 6 0.056505933458559074 7 0.03670872399267436 8 0.043251869494110834 9 0.041607694470672946 10-11 0.043453197048001185 12-13 0.042396227390076834 14-15 0.04467793966750083 16-17 0.03560142244627742 18-19 0.02977970067961471 20-21 0.024360634020732713 22-23 0.024780066424670944 24-25 0.02068640616223377 26-27 0.013321173149078354 28-29 0.018773794400275417 30-31 0.023806983247534243 32-33 0.0015267339503351766 34-35 0.0035735640815537655 36-37 0.004194324039382354 38-39 0.00630826335523106 40-41 0.006761250351484355 42-43 0.007717556232463531 44-45 0.008371870782607179 46-47 0.017347724226885415 48-49 0.02593769985954048 50-51 0.022263472001041534 52-53 0.02803486187923165 54-55 0.024578738870780593 56-57 0.015820990276550238 58-59 0.014847907099413532 60-61 0.01228098078731153 62-63 0.01865635332717271 64-65 0.020485078608343418 66-67 0.021458161785480122 68-69 0.028487848875484948 70-71 0.028336853210067185 72-73 0.025669263121020004 74-75 0.02548471286328718 76-77 0.01951199543120671 78-79 0.015166675726406592 80-81 0.013992264995379533 82-83 0.00887518966733306 84-85 0.01176088460642812 86-87 0.009781163659839649 88-89 0.007684001640148472 90-91 0.007851774601723767 92-93 0.004445983481745295 94-95 0.0016273977272803532 96-97 0.0010569696579243533 98-99 7.046464386162354E-4 100 0.11173679240914591 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2980218.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.8234017476817 #Duplication Level Percentage of deduplicated Percentage of total 1 87.87560134399163 70.14529429900563 2 8.396331161486167 13.404474310197784 3 1.89551101347852 4.539184114381537 4 0.7042586919457932 2.248652980059434 5 0.3361282942067773 1.3415451933615268 6 0.20164800451641868 0.9657737805679452 7 0.13546195011884546 0.7569123566102713 8 0.08396041520184 0.5361604762846909 9 0.06136808945939789 0.4408748693464669 >10 0.29375514018958854 4.009992812333744 >50 0.01265500068284734 0.6643830356999234 >100 0.002814210230754565 0.4029986908042747 >500 2.955659533109162E-4 0.19184544344739538 >1k 2.1111853806377747E-4 0.35190763789934165 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 4120 0.1382449203380424 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 3.355459231505883E-5 0.0 3 0.0 0.0 0.0 3.355459231505883E-5 0.0 4 0.0 0.0 0.0 3.355459231505883E-5 0.0 5 0.0 0.0 0.0 3.355459231505883E-5 0.0 6 0.0 0.0 0.0 3.355459231505883E-5 0.0 7 0.0 0.0 0.0 3.355459231505883E-5 0.0 8 0.0 0.0 0.0 6.710918463011766E-5 0.0 9 0.0 0.0 0.0 6.710918463011766E-5 0.0 10-11 0.0 0.0 0.0 6.710918463011766E-5 0.0 12-13 0.0 0.0 0.0 6.710918463011766E-5 0.0 14-15 0.0 1.6777296157529414E-5 0.0 1.0066377694517648E-4 0.0 16-17 0.0 3.355459231505883E-5 0.0 2.01327553890353E-4 0.0 18-19 0.0 3.355459231505883E-5 0.0 2.181048500478824E-4 0.0 20-21 0.0 3.355459231505883E-5 0.0 3.3554592315058833E-4 0.0 22-23 0.0 3.355459231505883E-5 0.0 4.529869962532942E-4 0.0 24-25 0.0 3.355459231505883E-5 0.0 5.704280693560001E-4 0.0 26-27 0.0 3.355459231505883E-5 0.0 7.214237347737648E-4 0.0 28-29 0.0 3.355459231505883E-5 0.0 0.001291851804129765 0.0 30-31 0.0 3.355459231505883E-5 0.0 0.003523232193081177 0.0 32-33 0.0 3.355459231505883E-5 0.0 0.009965713917572474 0.0 34-35 0.0 3.355459231505883E-5 0.0 0.019830764058199772 0.0 36-37 0.0 5.033188847258824E-5 0.0 0.03536654030007201 0.0 38-39 0.0 6.710918463011766E-5 0.0 0.060381488870948366 0.0 40-41 0.0 6.710918463011766E-5 0.0 0.09157048242779556 0.0 42-43 0.0 6.710918463011766E-5 0.0 0.12294402624237555 0.0 44-45 0.0 6.710918463011766E-5 0.0 0.16213579006636428 0.0 46-47 0.0 6.710918463011766E-5 0.0 0.20768614913405664 0.0 48-49 0.0 6.710918463011766E-5 0.0 0.25749794142576143 0.0 50-51 0.0 6.710918463011766E-5 0.0 0.3070916288674184 0.0 52-53 0.0 6.710918463011766E-5 0.0 0.36227215592953266 0.0 54-55 0.0 6.710918463011766E-5 0.0 0.42530445759337066 0.0 56-57 0.0 6.710918463011766E-5 0.0 0.5001815303444245 0.0 58-59 0.0 6.710918463011766E-5 0.0 0.5862658369287079 0.0 60-61 0.0 6.710918463011766E-5 0.0 0.673893654759484 0.0 62-63 0.0 6.710918463011766E-5 0.0 0.7667559889914093 0.0 64-65 0.0 1.006637769451765E-4 0.0 0.8731743785186185 0.0 66-67 0.0 1.006637769451765E-4 0.0 0.9945748935145013 0.0 68-69 0.0 1.006637769451765E-4 0.0 1.1309407566829004 0.0 70-71 0.0 1.006637769451765E-4 0.0 1.2834631560510004 0.0 72-73 0.0 1.006637769451765E-4 0.0 1.4463035925559808 0.0 74-75 0.0 1.006637769451765E-4 0.0 1.6204519266711361 0.0 76-77 0.0 1.006637769451765E-4 0.0 1.8160584225717717 0.0 78-79 0.0 1.006637769451765E-4 0.0 2.03324052133099 0.0 80-81 0.0 1.006637769451765E-4 0.0 2.2763267653574335 0.0 82-83 0.0 1.006637769451765E-4 0.0 2.538874672926611 0.0 84-85 0.0 1.006637769451765E-4 0.0 2.8105662069016426 0.0 86-87 0.0 1.006637769451765E-4 0.0 3.100125561284443 0.0 88 0.0 1.006637769451765E-4 0.0 3.343278914495517 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 7585 0.0 25.876318 1 TATCAAC 8700 0.0 22.370596 2 CAACGCA 8470 0.0 22.205894 5 TCAACGC 8540 0.0 22.133997 4 ATCAACG 8610 0.0 21.95331 3 AACGCAG 8670 0.0 21.551846 6 TAGACCG 185 7.1587074E-6 20.333397 5 CGCAGAG 9800 0.0 18.921478 8 ACGCAGA 9830 0.0 18.911488 7 GTACATG 10590 0.0 17.024593 1 GGTATCA 5505 0.0 16.692677 1 GCAGAGT 11110 0.0 16.394634 9 ACATGGG 10880 0.0 16.03327 3 TACATGG 11205 0.0 15.899686 2 GTATATA 1555 0.0 15.737335 1 GTATAAG 950 0.0 14.719732 1 GAGTACT 3390 0.0 14.40183 12-13 CATGGGG 5580 0.0 14.24112 4 AGAGTAC 10005 0.0 14.0997505 10-11 GTATTAG 1285 0.0 13.99152 1 >>END_MODULE