FastQCFastQC Report
Wed 25 May 2016
SRR1295128_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295128_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1873195
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT61200.32671451717520067No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA50440.2692725530443974No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG20550.10970560993382963No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC19140.10217836370479313No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTTC1854.065674E-621.7051018
CTGTGCG2951.5133992E-920.425359
CATGGGT21350.019.7938234
CTATTAG6301.8189894E-1216.8029441
GTACTGT6800.015.4884086
GTACATG201150.015.3855621
TAGACTG7850.015.3211395
TACATGG202800.015.1038622
CATGGGG29800.014.9374734
ATGGGTA13800.014.8886145
GTATAGA7351.6370905E-1114.4025231
TTATCGT2350.00495556414.3863194
ACATGGG202900.014.3835883
CACCGTC2801.5142722E-414.2447267
GTGCTAG7101.4551915E-1014.0326161
ATCGTCC2150.00591585114.0073618
AACCGTT2150.00618553113.9134547
GTCCTAG5853.25781E-813.8377181
TGTACTG8050.013.695455
CTTACAC6602.237357E-1013.6582663
Produced by FastQC (version 0.11.5)