##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295128_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1873195 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.232678925579023 33.0 27.0 33.0 6.0 33.0 2 22.39362532998433 27.0 2.0 33.0 2.0 33.0 3 24.70742554832786 27.0 15.0 33.0 15.0 33.0 4 24.804215791735512 33.0 6.0 37.0 2.0 37.0 5 26.587662790045883 33.0 15.0 37.0 2.0 37.0 6 27.531420914533726 33.0 15.0 37.0 2.0 37.0 7 27.416044245260103 33.0 15.0 37.0 6.0 37.0 8 28.308342164056597 33.0 22.0 37.0 6.0 37.0 9 29.14808602414591 33.0 27.0 37.0 15.0 37.0 10-11 28.685171591852423 33.0 24.5 37.0 10.5 37.0 12-13 27.493737704830515 33.0 15.0 37.0 4.0 37.0 14-15 29.255981624977643 33.0 22.0 37.0 6.0 40.0 16-17 29.68407159959321 33.0 27.0 38.5 6.0 40.0 18-19 29.83609421336273 35.0 27.0 40.0 10.5 40.0 20-21 29.36658436521558 33.0 24.5 40.0 6.0 40.0 22-23 30.138992470084535 33.0 27.0 40.0 15.0 40.0 24-25 30.312213090468425 33.0 27.0 40.0 15.0 40.0 26-27 28.852002327574013 33.0 18.5 38.5 4.0 40.0 28-29 29.60174007511231 33.0 24.5 38.5 10.5 40.0 30-31 30.304002519759024 33.0 27.0 40.0 15.0 40.0 32-33 29.064460453930316 33.0 22.0 37.0 6.0 40.0 34-35 28.66124642655997 33.0 22.0 37.0 6.0 40.0 36-37 29.01898520976193 33.0 22.0 37.0 6.0 40.0 38-39 29.11131916324782 33.0 24.5 37.0 6.0 40.0 40-41 28.149539423284814 33.0 22.0 37.0 6.0 40.0 42-43 28.793731565587137 33.0 22.0 37.0 6.0 40.0 44-45 28.693148604389826 33.0 22.0 37.0 6.0 40.0 46-47 28.397857937908228 33.0 22.0 37.0 6.0 40.0 48-49 28.328947333299524 33.0 22.0 37.0 6.0 40.0 50-51 28.330124466486403 33.0 22.0 37.0 6.0 40.0 52-53 28.54413635526467 33.0 22.0 37.0 6.0 40.0 54-55 28.514630083894097 33.0 22.0 37.0 6.0 40.0 56-57 28.453915903042663 33.0 22.0 37.0 6.0 40.0 58-59 28.377351530406607 33.0 22.0 37.0 6.0 40.0 60-61 28.327913004252093 33.0 22.0 37.0 6.0 40.0 62-63 28.265068239024767 33.0 22.0 37.0 6.0 40.0 64-65 27.828841631543966 33.0 22.0 37.0 6.0 40.0 66-67 28.05771422622845 33.0 22.0 37.0 10.5 40.0 68-69 27.6593899727471 33.0 22.0 37.0 6.0 38.5 70-71 27.773946385720656 33.0 22.0 37.0 10.5 37.0 72-73 27.793908802874235 33.0 22.0 37.0 10.5 37.0 74-75 27.89415143644949 33.0 22.0 37.0 15.0 37.0 76-77 27.61198086691455 33.0 22.0 37.0 10.5 37.0 78-79 27.175018084075603 30.0 22.0 37.0 6.0 37.0 80-81 27.02640248345741 27.0 22.0 33.0 6.0 37.0 82-83 27.14504469636103 27.0 22.0 33.0 15.0 37.0 84-85 27.086522225395647 27.0 22.0 33.0 10.5 37.0 86-87 26.834550860962153 27.0 22.0 33.0 15.0 37.0 88-89 26.769250665307133 27.0 22.0 33.0 10.5 37.0 90-91 26.915595279722613 27.0 22.0 33.0 15.0 37.0 92-93 26.818693195316023 27.0 22.0 33.0 15.0 37.0 94-95 26.71737832953857 27.0 22.0 33.0 15.0 37.0 96-97 26.812750941573086 27.0 22.0 33.0 15.0 37.0 98-99 26.880896809995754 27.0 22.0 33.0 15.0 37.0 100 26.479632926630703 27.0 22.0 33.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.309714975706687 1101 2 11.282149458173055 1101 3 8.343977770922653 1101 4 12.778038804778745 1101 5 10.739249633636955 1101 6 9.741352627498841 1101 7 9.328067696740597 1101 8 8.746903200819332 1101 9 7.108577701813324 1101 10-11 7.637386328823034 1101 12-13 9.035070470914214 1101 14-15 7.698056392607008 1101 16-17 7.925432814731348 1101 18-19 8.04813988126871 1101 20-21 7.21793198088119 1101 22-23 7.371354633419649 1101 24-25 7.349632076468815 1101 26-27 8.574759613119006 1101 28-29 8.164597558437489 1101 30-31 5.31161592056473 1101 32-33 6.983532733160601 1101 34-35 8.771167229794752 1101 36-37 8.202604296531096 1101 38-39 8.14638174415505 1101 40-41 9.073203775461067 1101 42-43 8.603990619644232 1101 44-45 8.429846485457443 1101 46-47 7.426631382924427 1101 48-49 5.57860864269535 1101 50-51 6.868134946161664 1101 52-53 5.975500031199868 1101 54-55 4.060397645429816 1101 56-57 6.118667104111207 1101 58-59 6.207564683713191 1101 60-61 6.647419709757319 1101 62-63 6.3706533519937025 1101 64-65 6.801934399698343 1101 66-67 5.897159349181635 1101 68-69 6.754537521927343 1101 70-71 6.30516997474793 1101 72-73 5.550227852766334 1101 74-75 4.937484527836833 1101 76-77 5.079755240663687 1101 78-79 5.210479182619046 1101 80-81 5.047298576741952 1101 82-83 4.577338224414035 1101 84-85 4.32486313647237 1101 86-87 4.200373742024176 1101 88-89 4.069208982822747 1101 90-91 3.9062908044545956 1101 92-93 3.8738754278969125 1101 94-95 3.8063042660021082 1101 96-97 3.9434700739209028 1101 98-99 3.655389219732566 1101 100 3.7663319453643993 1102 1 6.8562213487598065 1102 2 10.492583149371388 1102 3 8.14089855483454 1102 4 12.594964197341419 1102 5 10.432814008276143 1102 6 9.467987626034123 1102 7 6.769881151794607 1102 8 7.828226949956743 1102 9 6.488743159730582 1102 10-11 7.45552048100901 1102 12-13 9.029240987315987 1102 14-15 6.115845815263185 1102 16-17 7.25460744337337 1102 18-19 7.426980566584888 1102 20-21 6.936805999244697 1102 22-23 6.431531018496933 1102 24-25 6.779336209668628 1102 26-27 8.352789586508955 1102 28-29 7.640331112234296 1102 30-31 4.391492786240949 1102 32-33 4.434895900970648 1102 34-35 4.931387740251857 1102 36-37 5.071530407356892 1102 38-39 4.646088494235027 1102 40-41 6.305787365477457 1102 42-43 7.033833410721595 1102 44-45 6.38027882225175 1102 46-47 5.524122027731053 1102 48-49 3.8722790408165864 1102 50-51 5.138812129785759 1102 52-53 5.844676584198741 1102 54-55 5.249440241129797 1102 56-57 6.360745863011353 1102 58-59 6.028524943905847 1102 60-61 6.136107954143281 1102 62-63 4.3775064218331465 1102 64-65 6.278058003467141 1102 66-67 3.868262434648816 1102 68-69 6.080313362546466 1102 70-71 5.397864681050631 1102 72-73 4.955768437697273 1102 74-75 4.162716492507112 1102 76-77 3.353718845805272 1102 78-79 4.411689413088354 1102 80-81 4.460681038374325 1102 82-83 3.8899960798960613 1102 84-85 3.1496145941420366 1102 86-87 3.383167693660308 1102 88-89 3.4734059805809316 1102 90-91 3.3063759296664905 1102 92-93 3.2556913588750156 1102 94-95 3.1582622684461654 1102 96-97 3.2612417544972807 1102 98-99 3.108063248392 1102 100 3.2934163486360504 1103 1 6.611830918023546 1103 2 10.974385126773086 1103 3 7.962996174307367 1103 4 12.015066305573285 1103 5 10.294441414186775 1103 6 9.013553634570194 1103 7 7.976128041568181 1103 8 7.941947049142868 1103 9 7.028346939458324 1103 10-11 7.334038611120825 1103 12-13 8.722079101535535 1103 14-15 8.533983464353398 1103 16-17 7.948265848459297 1103 18-19 7.196328965370093 1103 20-21 8.356711304137185 1103 22-23 6.73685238389637 1103 24-25 5.128578549732758 1103 26-27 5.848667501844581 1103 28-29 6.925818607619117 1103 30-31 6.065063079113909 1103 32-33 7.964951943990094 1103 34-35 7.183814218512378 1103 36-37 4.110373717911994 1103 38-39 6.5012024526803955 1103 40-41 6.833685908386542 1103 42-43 7.527322471503442 1103 44-45 5.800156254248154 1103 46-47 6.503591398020561 1103 48-49 5.688816785671669 1103 50-51 6.513956071817159 1103 52-53 6.11828740407449 1103 54-55 5.051061503089123 1103 56-57 6.030068909688836 1103 58-59 5.5995721721924525 1103 60-61 5.13429029016412 1103 62-63 2.9562184143645887 1103 64-65 5.216431782703271 1103 66-67 4.366806303138411 1103 68-69 5.286269828657041 1103 70-71 3.7245136403287376 1103 72-73 4.433368097879956 1103 74-75 3.336229785464358 1103 76-77 3.6784164515329074 1103 78-79 3.0213538648056044 1103 80-81 4.243870164220102 1103 82-83 3.497434462297395 1103 84-85 3.382828087608555 1103 86-87 2.883815040887992 1103 88-89 3.111265136485546 1103 90-91 2.9514488669843892 1103 92-93 2.4814843286596684 1103 94-95 2.85381402386799 1103 96-97 3.073159684400803 1103 98-99 2.970949167293348 1103 100 3.1143687024754776 1104 1 6.397416007633861 1104 2 9.86522620500281 1104 3 7.3422276365341865 1104 4 12.177338222281854 1104 5 9.594403982095233 1104 6 8.298930374531281 1104 7 3.775714356416124 1104 8 6.611285851048013 1104 9 6.722161511748375 1104 10-11 7.3079493063426995 1104 12-13 7.556100944049735 1104 14-15 7.300772581790724 1104 16-17 7.24537363974434 1104 18-19 6.91561368653322 1104 20-21 7.640483687394408 1104 22-23 6.517043407356219 1104 24-25 6.102455335807711 1104 26-27 8.429718767837592 1104 28-29 7.214101422656672 1104 30-31 6.396942462507152 1104 32-33 7.726619878573331 1104 34-35 8.134840264859125 1104 36-37 6.765237693607158 1104 38-39 7.379131552486257 1104 40-41 7.261056379953608 1104 42-43 7.488198432681241 1104 44-45 6.891286909508128 1104 46-47 6.893086479551933 1104 48-49 5.829915515453703 1104 50-51 5.869962408995544 1104 52-53 6.134992924032783 1104 54-55 5.028278581654778 1104 56-57 6.246910272330318 1104 58-59 4.091815897615568 1104 60-61 4.3584336156589565 1104 62-63 4.108367822767512 1104 64-65 3.011560799761792 1104 66-67 3.382819438891442 1104 68-69 4.0611987836808225 1104 70-71 2.695029814186764 1104 72-73 2.4213393995996135 1104 74-75 3.346162718130902 1104 76-77 4.03305524859481 1104 78-79 4.573506712426848 1104 80-81 3.1686885941022993 1104 82-83 3.2187466155417503 1104 84-85 3.0974403988250963 1104 86-87 0.7862874073484285 1104 88-89 0.3911019655417114 1104 90-91 2.3367414684658065 1104 92-93 2.7045036609309214 1104 94-95 2.5709120719892233 1104 96-97 3.0213735257217103 1104 98-99 2.6607319323527427 1104 100 1.1026952612786367 1105 1 5.789915212090872 1105 2 -2.7225419914486437 1105 3 0.48202613478175493 1105 4 8.450842545515336 1105 5 8.59377112820616 1105 6 8.048740495986415 1105 7 5.36076808410008 1105 8 6.026362364157354 1105 9 4.936550836837004 1105 10-11 4.978847381509098 1105 12-13 5.14121710675316 1105 14-15 5.865728051523611 1105 16-17 6.074590588233885 1105 18-19 6.128781612567181 1105 20-21 7.093153914572838 1105 22-23 2.236744408509587 1105 24-25 4.012643343539807 1105 26-27 6.908719565842169 1105 28-29 5.956302975431267 1105 30-31 5.385623150124832 1105 32-33 7.2119040116194455 1105 34-35 6.268063393296689 1105 36-37 6.374367170410647 1105 38-39 4.316446792941637 1105 40-41 5.529857041272493 1105 42-43 5.8431419659125545 1105 44-45 6.160764285876716 1105 46-47 5.520566705105715 1105 48-49 5.199595503530627 1105 50-51 6.0650487953416565 1105 52-53 5.7345259816972245 1105 54-55 5.45774759754908 1105 56-57 5.1909872925283835 1105 58-59 2.1753342608960757 1105 60-61 4.6534632510339655 1105 62-63 -0.8586425423408457 1105 64-65 -0.30458104723168233 1105 66-67 0.8275058159282445 1105 68-69 2.9507881581803908 1105 70-71 3.1614037449031365 1105 72-73 2.834368760171028 1105 74-75 2.8088335137141733 1105 76-77 1.93901714398773 1105 78-79 3.462331649555452 1105 80-81 1.6938548301388963 1105 82-83 0.9836248359243918 1105 84-85 1.4985213974094513 1105 86-87 -0.14391874458215526 1105 88-89 1.7499052809083828 1105 90-91 1.483976223691439 1105 92-93 1.6551665686662815 1105 94-95 1.1806289750511354 1105 96-97 2.124784939380344 1105 98-99 2.0558402045228803 1105 100 2.1206537211790213 1106 1 6.090547299258841 1106 2 10.291169899391612 1106 3 6.6076177326829 1106 4 11.698339973283318 1106 5 9.635342541624073 1106 6 9.248918070452802 1106 7 6.610962714003286 1106 8 7.381113470750584 1106 9 6.677875990158114 1106 10-11 5.608086183292762 1106 12-13 7.905060941421496 1106 14-15 7.736350796085119 1106 16-17 7.649423845438843 1106 18-19 7.195566157094248 1106 20-21 8.133333833195366 1106 22-23 6.093207769627462 1106 24-25 5.03340134096247 1106 26-27 7.614893695821614 1106 28-29 6.149484635471108 1106 30-31 5.2691417841559804 1106 32-33 7.2312636178506935 1106 34-35 6.776493850874115 1106 36-37 5.866300582769046 1106 38-39 7.169170018921079 1106 40-41 6.648637473939395 1106 42-43 6.001594241460378 1106 44-45 4.317972828872346 1106 46-47 5.901012422582944 1106 48-49 5.766476350197767 1106 50-51 6.435717773589555 1106 52-53 5.536800091482281 1106 54-55 5.0658722324343906 1106 56-57 4.867349415789555 1106 58-59 4.967554971546495 1106 60-61 4.9296173690947 1106 62-63 4.780147984423202 1106 64-65 5.176956929489425 1106 66-67 3.0876411213628217 1106 68-69 3.2615193215096276 1106 70-71 2.813467483683734 1106 72-73 2.8186080521245387 1106 74-75 2.8461890952952906 1106 76-77 3.5007199724069444 1106 78-79 4.199375192410372 1106 80-81 3.679583310677298 1106 82-83 2.1118739584894293 1106 84-85 2.6555130904994186 1106 86-87 3.330508707651024 1106 88-89 3.351635441322262 1106 90-91 3.1928061155788328 1106 92-93 2.990669864865257 1106 94-95 1.5386826203530966 1106 96-97 1.6219099772254744 1106 98-99 1.9777803308166142 1106 100 2.930227650699088 1107 1 6.000962086864973 1107 2 10.320499043000062 1107 3 6.109972425057737 1107 4 11.46565164450412 1107 5 9.321788002762172 1107 6 7.963006998997855 1107 7 3.726281077807162 1107 8 6.2560097466089815 1107 9 5.586222529882008 1107 10-11 4.5249550217397605 1107 12-13 6.175599774263098 1107 14-15 5.951817073481067 1107 16-17 6.876688948242876 1107 18-19 6.204177379519727 1107 20-21 5.365218761445014 1107 22-23 5.601635363699536 1107 24-25 4.493766581903941 1107 26-27 7.191128912029093 1107 28-29 6.542587479455715 1107 30-31 5.002774738105732 1107 32-33 5.023294171526352 1107 34-35 6.710742672589657 1107 36-37 6.585698722453717 1107 38-39 6.362766241146794 1107 40-41 7.67303068619767 1107 42-43 6.009771559802392 1107 44-45 3.950597294815598 1107 46-47 3.376975631536091 1107 48-49 3.794064126790431 1107 50-51 4.77029066136409 1107 52-53 3.827989259861038 1107 54-55 5.13458189442747 1107 56-57 5.166886516162563 1107 58-59 2.8680061276756916 1107 60-61 2.7630947216986677 1107 62-63 0.2005874212680041 1107 64-65 2.1456793035162 1107 66-67 4.079260713149154 1107 68-69 4.75561827440125 1107 70-71 3.9679022279721003 1107 72-73 2.19235404026724 1107 74-75 2.4286608352337176 1107 76-77 2.9717523650901363 1107 78-79 3.864853576505073 1107 80-81 2.017708273600533 1107 82-83 1.574738990590344 1107 84-85 2.0319967058986528 1107 86-87 2.029160838611606 1107 88-89 2.3574890907050055 1107 90-91 1.3529051302146193 1107 92-93 2.0446164670597184 1107 94-95 2.1617516927015217 1107 96-97 2.065540824609645 1107 98-99 1.5849888105905592 1107 100 -0.23492491186530273 1108 1 4.299778102695868 1108 2 -3.360377441444017 1108 3 -0.011278700431919475 1108 4 6.660385519469184 1108 5 6.931931955924664 1108 6 6.789488752852375 1108 7 4.2698962720008495 1108 8 4.88701745683807 1108 9 4.809617644721236 1108 10-11 3.3767807671341217 1108 12-13 2.6023283144905136 1108 14-15 3.969582133139756 1108 16-17 4.48361040608707 1108 18-19 3.831216831056892 1108 20-21 4.542656877964557 1108 22-23 3.848548272345724 1108 24-25 2.9380987683607316 1108 26-27 4.737999158010776 1108 28-29 6.116827598358849 1108 30-31 4.353377800580343 1108 32-33 4.405808585650359 1108 34-35 2.914126331360716 1108 36-37 1.0881790850101822 1108 38-39 2.470970257246236 1108 40-41 5.298880606467051 1108 42-43 2.932890974314457 1108 44-45 3.557533453661648 1108 46-47 4.74443967181422 1108 48-49 4.6509398151187185 1108 50-51 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-6.003484688183772 2213 50-51 -23.82336751689252 2213 52-53 -18.23528132945428 2213 54-55 -18.082012669136052 2213 56-57 -17.926576814687557 2213 58-59 -18.40986271156411 2213 60-61 -19.77425945676636 2213 62-63 -17.89520012202022 2213 64-65 -17.984197076543087 2213 66-67 -8.766870856379661 2213 68-69 -23.228221098762315 2213 70-71 -17.850504331182428 2213 72-73 -9.544582964705594 2213 74-75 -4.362686513259824 2213 76-77 -3.0583657094918344 2213 78-79 -4.826852623069534 2213 80-81 -3.2662066504237117 2213 82-83 -3.3980948769128005 2213 84-85 -3.0592805037314115 2213 86-87 -2.9966574119455665 2213 88-89 -2.621009463097689 2213 90-91 -2.6381276246191128 2213 92-93 -3.7209944311209213 2213 94-95 -3.8066081278868964 2213 96-97 -3.374536115692152 2213 98-99 -3.761003349612004 2213 100 -3.2299477352964914 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 504.0 3 496.0 4 2671.0 5 10107.0 6 15193.0 7 16763.0 8 15152.0 9 15612.0 10 17933.0 11 21610.0 12 26834.0 13 36029.0 14 27946.0 15 31012.0 16 35051.0 17 37448.0 18 37648.0 19 37832.0 20 37509.0 21 36499.0 22 38399.0 23 42187.0 24 46594.0 25 50487.0 26 55860.0 27 59820.0 28 65432.0 29 67319.0 30 71455.0 31 77665.0 32 87684.0 33 106304.0 34 129687.0 35 151894.0 36 167093.0 37 147851.0 38 47540.0 39 75.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.59353738527171 21.206443990478352 13.690045920416253 23.509972703833686 2 14.18651522664279 21.580631467800565 41.14614509276534 23.086708212791308 3 18.198157832631605 28.46478071960589 29.185834533284538 24.151226914477967 4 11.6398736597595 18.286050167839893 39.2649037576307 30.809172414769908 5 11.967328521074245 39.30213446051736 35.95685538242852 12.773681635979873 6 29.186597746139288 38.29308043696741 19.73916194558674 12.781159871306564 7 25.517783568943177 32.56855008953659 25.016902761803543 16.896763579716687 8 23.890465797353176 37.54042881629579 21.92481865227324 16.644286734077795 9 25.13125573210777 17.879712053941006 22.406177086035157 34.58285512791606 10-11 22.22532475730012 28.909862792862455 29.128989317725697 19.735823132111726 12-13 23.711355252004353 26.28527909045041 29.26224786608376 20.741117791461473 14-15 21.62322540421007 27.505949177583496 26.586902815785006 24.28392260242143 16-17 20.231039509076677 31.21930602044874 28.205023784334426 20.34463068614016 18-19 20.66097456848366 30.195779008813396 29.622201533993604 19.52104488870934 20-21 21.760371821557353 29.403260431258932 28.762443971535323 20.073923775648392 22-23 20.52844361663369 29.577558851262907 28.596623949282506 21.297373582820896 24-25 20.750291757830684 29.707970832413565 28.143402683465435 21.398334726290315 26-27 21.149308756307438 28.922008859648233 28.44768587246404 21.480996511580283 28-29 20.913078582497864 28.990821706771975 29.09197478910025 21.004124921629916 30-31 21.532354519237217 29.573673738570566 28.456635219422772 20.437336522769446 32-33 20.24883502661202 29.388781015544335 29.117022413770826 21.245361544072818 34-35 20.041441330515855 29.15044116316664 29.210993253716726 21.59712425260078 36-37 21.37491174448101 29.325842825832478 28.563596869487622 20.73564856019889 38-39 21.410688429793588 28.78814523164621 28.59416465311305 21.207001685447146 40-41 20.831311575550338 29.459982992283713 28.3631115138482 21.345593918317757 42-43 21.17898756520455 29.141211565515906 28.711751528450762 20.96804934082878 44-45 21.77279294167364 28.632048434374756 28.576447164764712 21.018711459186893 46-47 21.075280805878954 29.151306521329186 29.387038118379472 20.386374554412388 48-49 21.301669061327345 28.939685452666037 29.241883186029916 20.516762299976698 50-51 20.81264187103626 29.209033764234288 29.114181413056965 20.864142951672484 52-53 20.252341398523722 29.352330648917874 28.510530134728395 21.884797817830012 54-55 20.802761597179625 29.554162396124124 28.180260480340678 21.46281552635557 56-57 21.33860416928983 28.834390908878365 28.27004076785421 21.55696415397759 58-59 21.04428504168167 28.40986512310632 29.028581715456838 21.517268119755173 60-61 21.57600923108263 29.077993997563865 28.448142830632793 20.89785394072071 62-63 20.482148449517755 29.267520097566496 28.884345351174534 21.365986101741214 64-65 20.241668135661715 29.05922567659483 29.122170596426937 21.576935591316516 66-67 21.17129331217115 28.88092215056971 28.647193182407023 21.300591354852116 68-69 21.85313443938506 28.605112483657187 28.34049975204003 21.201253324917722 70-71 21.11870016004682 29.13811183110717 28.327958708413103 21.4152293004329 72-73 21.366972867908892 28.913861600801244 28.610952747792766 21.1082127834971 74-75 21.803373381069267 28.461593355029034 28.25114414088114 21.483889123020557 76-77 21.180339289804838 28.909950299482333 29.006636021124276 20.903074389588557 78-79 21.542846294690598 28.729760515067216 28.956076115585503 20.77131707465668 80-81 21.06382961287797 28.806980384808384 28.858416006831135 21.270773995482514 82-83 20.47298940060241 29.11488958635506 28.578713592252612 21.833407420789918 84-85 20.808598800192573 29.034636460514 28.152875372573206 22.00388936672022 86-87 21.363829541040023 28.631539326636045 28.32883777908613 21.675793353237808 88-89 21.198062036057284 28.240072160866404 28.920577556107848 21.641288246968465 90-91 21.619358090356343 28.501281460113887 28.50793003978424 21.371430409745532 92-93 20.692294539546786 28.82465691600412 28.813909997100208 21.669138547348886 94-95 20.382985678968076 28.667803431175066 29.025554119017794 21.923656770839063 96-97 21.301944307842298 28.494691485546536 28.706028127395754 21.497336079215405 98-99 21.5582564031597 28.286595939717422 28.472478119755216 21.682669537367655 100 21.000628640788428 28.4651340572455 28.75422292536461 21.780014376601464 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 270.0 1 307.5 2 302.0 3 253.5 4 292.5 5 378.0 6 544.0 7 811.5 8 1157.5 9 1670.0 10 2304.0 11 2989.0 12 3771.5 13 4513.5 14 5191.5 15 5905.0 16 6501.5 17 7125.0 18 7789.5 19 8415.0 20 9048.5 21 9769.0 22 10810.5 23 12068.0 24 13540.0 25 15753.0 26 18732.0 27 22261.0 28 26703.0 29 32244.0 30 38231.0 31 44442.5 32 50718.5 33 56573.5 34 62128.0 35 66932.0 36 70587.5 37 73486.5 38 75252.0 39 75746.0 40 75202.0 41 73724.0 42 71025.0 43 67482.0 44 63743.0 45 59837.5 46 56670.0 47 54582.5 48 53936.5 49 56464.5 50 61843.5 51 58104.0 52 45308.0 53 35465.5 54 29350.5 55 25430.0 56 22495.0 57 19766.5 58 17118.0 59 14813.5 60 12748.5 61 10670.5 62 8814.0 63 7076.5 64 5650.0 65 4588.0 66 3624.5 67 2829.0 68 2236.0 69 1825.0 70 1408.5 71 1074.5 72 806.0 73 610.0 74 452.0 75 327.5 76 247.0 77 158.5 78 98.0 79 70.0 80 46.0 81 30.0 82 16.0 83 9.0 84 8.0 85 5.5 86 2.5 87 4.5 88 4.0 89 0.5 90 1.0 91 2.0 92 1.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.5 99 0.5 100 2.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 8.274899303062416 2 25.173033239999036 3 6.350486735230448 4 24.61756517607617 5 13.019680278881804 6 10.282538657214012 7 8.329084798966472 8 8.838588614639693 9 5.528415354514612 10-11 7.178830821137148 12-13 10.381941015217317 14-15 7.696716038639864 16-17 8.23787699625506 18-19 6.9034724094394875 20-21 8.386900456172475 22-23 6.075288477707874 24-25 5.561433806944819 26-27 10.061846203945665 28-29 6.507010749014384 30-31 4.481914589778427 32-33 9.106419780108316 34-35 7.648936709739243 36-37 7.149656068909003 38-39 6.498575962459861 40-41 9.067422238474904 42-43 6.7265287383321 44-45 6.77089144483089 46-47 6.153310253337212 48-49 4.1205266936971325 50-51 6.656434594369514 52-53 4.648902009667974 54-55 5.1770637867387 56-57 5.239417145572137 58-59 3.621886669567237 60-61 4.3467177736434275 62-63 3.2616732374365722 64-65 4.756338768788087 66-67 2.642623966004607 68-69 5.26987313120097 70-71 2.584701539348546 72-73 1.631517273962401 74-75 0.8783121885334949 76-77 1.303735062286628 78-79 1.8595501269221837 80-81 2.282277072061371 82-83 0.8624569251999925 84-85 1.311395770328236 86-87 0.5765283379466632 88-89 0.8369924113613372 90-91 0.4342847381078852 92-93 0.4027343656159663 94-95 0.5501562837825213 96-97 0.5619810003763623 98-99 0.4720277387031248 100 0.5576568376490435 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1873195.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 95.37131206169211 #Duplication Level Percentage of deduplicated Percentage of total 1 98.39102730466853 93.83681369144008 2 1.3151393025996339 2.508531216656516 3 0.15434529301294847 0.4416033931557365 4 0.047544272897528606 0.18137438749025805 5 0.022859436510561243 0.10900672265015872 6 0.013504832133371397 0.07727841358395185 7 0.009132876922578081 0.06097101185029544 8 0.007122277552191858 0.05434087640160594 9 0.005227748313990297 0.0448719494250198 >10 0.027830772659851316 0.487393161920311 >50 0.003027655913618516 0.20185662753428157 >100 0.002215917049132736 0.5105067552784422 >500 5.111548830558026E-4 0.29872617547633556 >1k 3.9756490904336374E-4 0.5820150055290968 >5k 1.1358997401238965E-4 0.6047106116078954 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 6120 0.32671451717520067 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 5044 0.2692725530443974 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 2055 0.10970560993382963 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 1914 0.10217836370479313 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 5.338472502862756E-5 0.0 6 0.0 0.0 0.0 5.338472502862756E-5 0.0 7 0.0 0.0 0.0 5.338472502862756E-5 0.0 8 0.0 0.0 0.0 5.338472502862756E-5 0.0 9 0.0 0.0 0.0 1.0676945005725512E-4 0.0 10-11 5.338472502862756E-5 0.0 0.0 1.0676945005725512E-4 0.0 12-13 5.338472502862756E-5 0.0 0.0 1.0676945005725512E-4 0.0 14-15 5.338472502862756E-5 0.0 0.0 1.0676945005725512E-4 0.0 16-17 8.007708754294133E-5 0.0 0.0 1.0676945005725512E-4 0.0 18-19 1.0676945005725512E-4 0.0 0.0 1.6015417508588267E-4 0.0 20-21 1.0676945005725512E-4 0.0 0.0 2.936159876574516E-4 0.0 22-23 1.0676945005725512E-4 0.0 0.0 3.2030835017176533E-4 0.0 24-25 1.0676945005725512E-4 2.669236251431378E-5 0.0 3.736930752003929E-4 0.0 26-27 1.0676945005725512E-4 5.338472502862756E-5 0.0 4.5377016274333426E-4 0.0 28-29 1.0676945005725512E-4 5.338472502862756E-5 0.0 7.740785129150996E-4 0.0 30-31 1.6015417508588267E-4 5.338472502862756E-5 0.0 0.002535774438859809 0.0 32-33 1.6015417508588267E-4 5.338472502862756E-5 0.0 0.005872319753149032 5.338472502862756E-5 34-35 1.6015417508588267E-4 5.338472502862756E-5 0.0 0.01187810131886963 5.338472502862756E-5 36-37 1.6015417508588267E-4 5.338472502862756E-5 0.0 0.021167043473850827 5.338472502862756E-5 38-39 1.6015417508588267E-4 5.338472502862756E-5 0.0 0.04006523613398498 5.338472502862756E-5 40-41 1.6015417508588267E-4 5.338472502862756E-5 0.0 0.06512936453492563 5.338472502862756E-5 42-43 1.6015417508588267E-4 1.0676945005725512E-4 0.0 0.0875509490469492 1.0676945005725512E-4 44-45 1.6015417508588267E-4 1.0676945005725512E-4 0.0 0.11501739007417808 1.0676945005725512E-4 46-47 1.6015417508588267E-4 1.0676945005725512E-4 0.0 0.14945053771764283 1.0676945005725512E-4 48-49 1.6015417508588267E-4 1.0676945005725512E-4 0.0 0.18465776387402272 1.0676945005725512E-4 50-51 1.6015417508588267E-4 1.0676945005725512E-4 0.0 0.22485646182057928 1.0676945005725512E-4 52-53 1.6015417508588267E-4 1.0676945005725512E-4 0.0 0.2697797079321694 1.0676945005725512E-4 54-55 1.6015417508588267E-4 1.0676945005725512E-4 0.0 0.316704881232333 1.0676945005725512E-4 56-57 1.6015417508588267E-4 1.0676945005725512E-4 0.0 0.3727321501498776 1.0676945005725512E-4 58-59 1.6015417508588267E-4 1.0676945005725512E-4 0.0 0.4381284383099464 1.0676945005725512E-4 60-61 1.6015417508588267E-4 1.6015417508588267E-4 0.0 0.5082492746350487 1.0676945005725512E-4 62-63 1.6015417508588267E-4 1.6015417508588267E-4 0.0 0.5787438040353514 1.0676945005725512E-4 64-65 1.6015417508588267E-4 1.6015417508588267E-4 0.0 0.6579667359778347 1.0676945005725512E-4 66-67 1.6015417508588267E-4 1.8684653760019646E-4 0.0 0.7527513152661629 1.0676945005725512E-4 68-69 1.6015417508588267E-4 2.1353890011451023E-4 0.0 0.8618430008621634 1.0676945005725512E-4 70-71 1.6015417508588267E-4 2.1353890011451023E-4 0.0 1.0081972245281456 1.0676945005725512E-4 72-73 1.6015417508588267E-4 2.1353890011451023E-4 0.0 1.1509212868921814 1.0676945005725512E-4 74-75 2.1353890011451023E-4 2.1353890011451023E-4 0.0 1.2866786426399814 1.0676945005725512E-4 76-77 2.1353890011451023E-4 2.1353890011451023E-4 0.0 1.440613497260029 1.0676945005725512E-4 78-79 2.1353890011451023E-4 2.669236251431378E-4 0.0 1.6141405459655829 1.0676945005725512E-4 80-81 2.1353890011451023E-4 2.669236251431378E-4 0.0 1.8125715688969914 1.0676945005725512E-4 82-83 2.1353890011451023E-4 2.669236251431378E-4 0.0 2.043060119208091 1.0676945005725512E-4 84-85 2.1353890011451023E-4 2.669236251431378E-4 0.0 2.2680233504787273 1.0676945005725512E-4 86-87 2.1353890011451023E-4 2.669236251431378E-4 0.0 2.497817899364455 1.0676945005725512E-4 88 2.1353890011451023E-4 2.669236251431378E-4 0.0 2.7048438630254723 1.0676945005725512E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCGTTC 185 4.065674E-6 21.705101 8 CTGTGCG 295 1.5133992E-9 20.42535 9 CATGGGT 2135 0.0 19.793823 4 CTATTAG 630 1.8189894E-12 16.802944 1 GTACTGT 680 0.0 15.488408 6 GTACATG 20115 0.0 15.385562 1 TAGACTG 785 0.0 15.321139 5 TACATGG 20280 0.0 15.103862 2 CATGGGG 2980 0.0 14.937473 4 ATGGGTA 1380 0.0 14.888614 5 GTATAGA 735 1.6370905E-11 14.402523 1 TTATCGT 235 0.004955564 14.386319 4 ACATGGG 20290 0.0 14.383588 3 CACCGTC 280 1.5142722E-4 14.244726 7 GTGCTAG 710 1.4551915E-10 14.032616 1 ATCGTCC 215 0.005915851 14.007361 8 AACCGTT 215 0.006185531 13.913454 7 GTCCTAG 585 3.25781E-8 13.837718 1 TGTACTG 805 0.0 13.69545 5 CTTACAC 660 2.237357E-10 13.658266 3 >>END_MODULE