FastQCFastQC Report
Wed 25 May 2016
SRR1295128_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295128_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1873195
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT169190.9032161627593497No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA130940.6990195895248493No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG51580.27535841169766095No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC45330.2419929585547687No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC42280.22571061742103732No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC40420.2157810585657126No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG38290.20441011213461494No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG30620.16346402803765758No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT28640.15289385248198933No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG26370.1407755199004909No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACT1405.205921E-420.1464614
CGTCTAC1350.00709117518.4751221
CATGGGT27200.018.3194544
GAGTACT40650.017.55549812-13
AGTACTT44100.017.08702912-13
CTAGTAC5950.016.5898723
CATGGGG29950.015.6956294
ATGGGTG12950.015.2459725
GTACTTT47500.015.02320914-15
AAGACCG1900.004024083714.8447615
CCTATAC5652.1827873E-1114.1429893
AGACCGT2000.0057000214.0863276
CCTAGAC7150.013.8055583
CATGGGC20850.013.7530594
TATACTG9950.013.7009445
CTAGACT8000.013.5149174
GTATAAG7800.013.4299931
CTTACAC8850.013.2781463
GTAATAC5352.1263986E-913.1788713
GTACATG256500.013.1659551
Produced by FastQC (version 0.11.5)