##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295128_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1873195 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.409055650906605 33.0 33.0 33.0 27.0 33.0 2 29.98383296987233 33.0 33.0 33.0 27.0 33.0 3 30.87718470314089 33.0 33.0 33.0 27.0 33.0 4 35.15757409132525 37.0 37.0 37.0 33.0 37.0 5 35.45591943177298 37.0 37.0 37.0 33.0 37.0 6 35.250114910620624 37.0 37.0 37.0 33.0 37.0 7 35.15323765011117 37.0 37.0 37.0 33.0 37.0 8 35.16315226124349 37.0 37.0 37.0 33.0 37.0 9 35.11267593603442 37.0 37.0 37.0 33.0 37.0 10-11 35.08720928680677 37.0 37.0 37.0 33.0 37.0 12-13 34.99640400492207 37.0 37.0 37.0 33.0 37.0 14-15 36.45619009232888 40.0 37.0 40.0 33.0 40.0 16-17 36.45594639105913 38.5 37.0 40.0 33.0 40.0 18-19 36.487356094800596 40.0 37.0 40.0 33.0 40.0 20-21 36.50004110623827 40.0 37.0 40.0 33.0 40.0 22-23 36.48045825447964 40.0 37.0 40.0 33.0 40.0 24-25 36.45495423594447 40.0 37.0 40.0 33.0 40.0 26-27 36.42412188800419 40.0 37.0 40.0 33.0 40.0 28-29 36.36311168885247 40.0 37.0 40.0 33.0 40.0 30-31 36.259892589933244 37.0 37.0 40.0 27.0 40.0 32-33 36.19712763486983 37.0 37.0 40.0 27.0 40.0 34-35 36.10923342204095 37.0 37.0 40.0 27.0 40.0 36-37 36.104865216915485 37.0 37.0 40.0 27.0 40.0 38-39 36.03134911207856 37.0 37.0 40.0 27.0 40.0 40-41 35.95823899807549 37.0 37.0 40.0 27.0 40.0 42-43 35.83634725695937 37.0 37.0 40.0 27.0 40.0 44-45 35.70714180851433 37.0 37.0 40.0 27.0 40.0 46-47 35.55902749046415 37.0 37.0 40.0 27.0 40.0 48-49 35.42326853317461 37.0 37.0 40.0 27.0 40.0 50-51 35.33577684117243 37.0 37.0 40.0 27.0 40.0 52-53 35.26542831899509 37.0 35.0 40.0 27.0 40.0 54-55 35.15701862326132 37.0 33.0 40.0 27.0 40.0 56-57 35.02217201092252 37.0 33.0 40.0 27.0 40.0 58-59 34.904864149220984 37.0 33.0 40.0 27.0 40.0 60-61 34.744222304671965 37.0 33.0 40.0 27.0 40.0 62-63 34.55735307856363 37.0 33.0 40.0 27.0 40.0 64-65 34.399542759830126 37.0 33.0 40.0 27.0 40.0 66-67 34.17756266699409 37.0 33.0 40.0 22.0 40.0 68-69 33.97151497841922 37.0 33.0 38.5 22.0 40.0 70-71 33.720185565304206 37.0 33.0 37.0 22.0 40.0 72-73 33.484083611156336 37.0 33.0 37.0 22.0 40.0 74-75 33.2560384797098 37.0 33.0 37.0 22.0 40.0 76-77 32.99789931107012 37.0 33.0 37.0 22.0 38.5 78-79 32.74717341227155 37.0 33.0 37.0 22.0 37.0 80-81 32.56202744508714 37.0 33.0 37.0 22.0 37.0 82-83 32.37934972066442 37.0 33.0 37.0 22.0 37.0 84-85 32.1897893705674 33.0 33.0 37.0 22.0 37.0 86-87 32.18771377245829 33.0 33.0 37.0 22.0 37.0 88-89 32.190807684197324 33.0 33.0 37.0 22.0 37.0 90-91 32.197016060794525 33.0 33.0 37.0 22.0 37.0 92-93 32.135679680972885 33.0 33.0 37.0 22.0 37.0 94-95 32.17274442863663 33.0 33.0 37.0 22.0 37.0 96-97 32.13226225780018 33.0 33.0 37.0 22.0 37.0 98-99 32.05490191891394 33.0 33.0 37.0 22.0 37.0 100 31.51239086160277 33.0 33.0 37.0 22.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5165433705233085 1101 2 4.083330080221231 1101 3 2.33917374567584 1101 4 1.2176315258789856 1101 5 0.49034000889308516 1101 6 0.47207654533051624 1101 7 0.4653705277429623 1101 8 0.6075556796669801 1101 9 0.5843874922178358 1101 10-11 0.5857480493333398 1101 12-13 0.6498749887032176 1101 14-15 0.8328854471934335 1101 16-17 0.8977823209418361 1101 18-19 0.8451910064589399 1101 20-21 0.8700199586038337 1101 22-23 0.9118178154695187 1101 24-25 0.8681125583027978 1101 26-27 0.9399433970812723 1101 28-29 0.7783035697193839 1101 30-31 0.7188489848471633 1101 32-33 0.909326592531329 1101 34-35 1.0187339048656199 1101 36-37 0.8980034735827687 1101 38-39 0.9012990775388445 1101 40-41 0.926409738592767 1101 42-43 0.9433364218701286 1101 44-45 1.0878695861722605 1101 46-47 0.9674314780438422 1101 48-49 0.8121714870095929 1101 50-51 0.8947479421465516 1101 52-53 0.8524417747655946 1101 54-55 0.8361383063290972 1101 56-57 0.8769847451482633 1101 58-59 0.8706121179261714 1101 60-61 0.926013018535869 1101 62-63 0.8888388265643457 1101 64-65 0.8624686736582277 1101 66-67 0.8791850180030991 1101 68-69 0.8505346994505487 1101 70-71 0.8457804486350256 1101 72-73 0.7361201056158393 1101 74-75 0.7070121203233981 1101 76-77 0.7578674151951503 1101 78-79 0.713818566296716 1101 80-81 0.6569750175304065 1101 82-83 0.7401575365658033 1101 84-85 0.7731696477093166 1101 86-87 0.8840272280074721 1101 88-89 0.8405870436466749 1101 90-91 0.6801193226471227 1101 92-93 0.7533978878032883 1101 94-95 0.8882532267059737 1101 96-97 0.8918695814261319 1101 98-99 0.8086742840385845 1101 100 0.892957019367806 1102 1 0.6677022745959604 1102 2 4.10603295584772 1102 3 2.352886381591208 1102 4 1.1263684736529243 1102 5 0.6212366611250317 1102 6 0.5041828458219015 1102 7 0.5535918091685019 1102 8 0.5410670057939839 1102 9 0.614257064949328 1102 10-11 0.5978045126596356 1102 12-13 0.691783466536954 1102 14-15 0.9366400880189687 1102 16-17 0.9514966667156628 1102 18-19 0.901163683043201 1102 20-21 0.880728943144014 1102 22-23 0.9348520297987477 1102 24-25 0.8391804353939989 1102 26-27 0.8764430226284006 1102 28-29 0.8797223845361941 1102 30-31 0.8595035051858986 1102 32-33 0.9762001684153461 1102 34-35 1.0017658271265049 1102 36-37 0.9244945130663993 1102 38-39 0.8469419414230757 1102 40-41 0.9571297360923268 1102 42-43 1.090778835185212 1102 44-45 0.9926615717775888 1102 46-47 1.0233898751649022 1102 48-49 0.9827049260804017 1102 50-51 0.9213552091063235 1102 52-53 0.9512153956329783 1102 54-55 0.9267075176001853 1102 56-57 0.9695597920008368 1102 58-59 0.851705710308174 1102 60-61 0.9116584502720713 1102 62-63 0.9967036545918049 1102 64-65 0.9767540815176972 1102 66-67 0.9314322868259453 1102 68-69 0.8576421337190538 1102 70-71 0.8853555518700276 1102 72-73 0.7745462636312794 1102 74-75 0.7374703991639251 1102 76-77 0.7998636228638958 1102 78-79 0.7635878886166836 1102 80-81 0.8036658898278759 1102 82-83 0.9395969297437716 1102 84-85 0.9396636840416619 1102 86-87 0.8467138875568772 1102 88-89 0.878934572686692 1102 90-91 0.9593822020487792 1102 92-93 0.8939974352730005 1102 94-95 0.9015479278607081 1102 96-97 0.8567566572249525 1102 98-99 1.0284948530843927 1102 100 0.9662725636359752 1103 1 0.5675655876693462 1103 2 4.043273916626763 1103 3 2.324871644134447 1103 4 1.2213544124195863 1103 5 0.5576884019488588 1103 6 0.5895667129771098 1103 7 0.5960171787838036 1103 8 0.5433834347122684 1103 9 0.5805409563843682 1103 10-11 0.5354791493651234 1103 12-13 0.6584409300269058 1103 14-15 0.8987819542250577 1103 16-17 0.8872177551544453 1103 18-19 0.8891081068085427 1103 20-21 0.8730602911161469 1103 22-23 0.9537499474018247 1103 24-25 0.8005378473143949 1103 26-27 0.7979345429973677 1103 28-29 0.8140923429594977 1103 30-31 0.9117595104311462 1103 32-33 0.9832372124661788 1103 34-35 1.0266391418352683 1103 36-37 1.0053931327278534 1103 38-39 0.8689891200463933 1103 40-41 0.8932429262616068 1103 42-43 0.9481987192640631 1103 44-45 1.0103286925205879 1103 46-47 0.9943880170554493 1103 48-49 0.9234605681314676 1103 50-51 0.8513027202730399 1103 52-53 0.9405143355474124 1103 54-55 0.951010237443036 1103 56-57 0.9364847600457651 1103 58-59 0.8731408452819664 1103 60-61 0.940306802303553 1103 62-63 1.0747917206530175 1103 64-65 0.8641578024521266 1103 66-67 0.8391164398209838 1103 68-69 0.8374296124491138 1103 70-71 0.8153591223597942 1103 72-73 0.7860161955173197 1103 74-75 0.7704242200770892 1103 76-77 0.7944436457290749 1103 78-79 0.7890122338638577 1103 80-81 0.8805112135021105 1103 82-83 0.9195027663846531 1103 84-85 0.8596470581256526 1103 86-87 0.9231571392183433 1103 88-89 0.844167038283576 1103 90-91 0.9143123247923555 1103 92-93 0.8659870245425196 1103 94-95 0.8221677696505232 1103 96-97 0.8017527104981177 1103 98-99 0.8248384495796692 1103 100 0.9828485655302046 1104 1 0.4560734074470929 1104 2 3.5583364691811106 1104 3 2.171118104444581 1104 4 1.0026223169644055 1104 5 0.38920013032738154 1104 6 0.3771158925110001 1104 7 0.33579316845429474 1104 8 0.27718697058936925 1104 9 0.3207243710567198 1104 10-11 0.4777644931942078 1104 12-13 0.3953901591782838 1104 14-15 0.6409518395020086 1104 16-17 0.7157253842938758 1104 18-19 0.6384989014508804 1104 20-21 0.5542731552868645 1104 22-23 0.614014075504592 1104 24-25 0.6477377246360589 1104 26-27 0.6602591879463802 1104 28-29 0.6388810435687162 1104 30-31 0.5954404967835032 1104 32-33 0.7199294019112088 1104 34-35 0.826945921397602 1104 36-37 0.6722564058950411 1104 38-39 0.5788447475865226 1104 40-41 0.751917330439575 1104 42-43 0.7571306268632085 1104 44-45 0.7460899744447858 1104 46-47 0.7409807435337612 1104 48-49 0.6525127015288348 1104 50-51 0.6444745387255892 1104 52-53 0.6294375536458929 1104 54-55 0.6503607919429228 1104 56-57 0.7118235230503913 1104 58-59 0.7850139350344847 1104 60-61 0.7952351957171544 1104 62-63 0.8275689216496929 1104 64-65 0.728587358501855 1104 66-67 0.7691036799345881 1104 68-69 0.7404608518867235 1104 70-71 0.673737876131348 1104 72-73 0.6502238446995321 1104 74-75 0.6321960783060661 1104 76-77 0.6543697901257559 1104 78-79 0.6776936246785894 1104 80-81 0.6264073694607433 1104 82-83 0.6742633465537295 1104 84-85 0.7097631864880967 1104 86-87 0.7503891339443562 1104 88-89 0.5843333534715747 1104 90-91 0.5624661858881339 1104 92-93 0.5116099043352662 1104 94-95 0.5494539546067791 1104 96-97 0.6430867884372411 1104 98-99 0.6418224109717947 1104 100 0.5820129514068952 1105 1 0.46822652944422316 1105 2 3.7576608282822797 1105 3 2.1893540533203684 1105 4 0.9958582994074092 1105 5 0.46173709118756534 1105 6 0.587994086934998 1105 7 0.4838805309350107 1105 8 0.5751671641424068 1105 9 0.5749467910022688 1105 10-11 0.4898325782472668 1105 12-13 0.47749937078287985 1105 14-15 0.5997066838105525 1105 16-17 0.6571494756181764 1105 18-19 0.663613443976736 1105 20-21 0.6120031701504232 1105 22-23 0.5149695327057202 1105 24-25 0.5944437290946851 1105 26-27 0.5579886120269677 1105 28-29 0.5757702041779851 1105 30-31 0.5842590337631606 1105 32-33 0.7283932632240067 1105 34-35 0.785151606072553 1105 36-37 0.6746557904065966 1105 38-39 0.6753334826530235 1105 40-41 0.6729923048277868 1105 42-43 0.7794145580584697 1105 44-45 0.728970023569282 1105 46-47 0.6932685282452908 1105 48-49 0.5762540316767115 1105 50-51 0.6225094960706059 1105 52-53 0.5716652440917258 1105 54-55 0.6228768516173702 1105 56-57 0.5179180313197875 1105 58-59 0.5751463375564114 1105 60-61 0.5872160328655838 1105 62-63 0.7075690380160395 1105 64-65 0.6905850195383323 1105 66-67 0.6384242750320794 1105 68-69 0.5240342206431663 1105 70-71 0.5355617932641863 1105 72-73 0.6852306454172421 1105 74-75 0.5846608741301296 1105 76-77 0.6270028874890272 1105 78-79 0.636418706056709 1105 80-81 0.5774927765393087 1105 82-83 0.6596853289618281 1105 84-85 0.6269947147854822 1105 86-87 0.7235219352447935 1105 88-89 0.6015448309526725 1105 90-91 0.5971059850577163 1105 92-93 0.5642614233860073 1105 94-95 0.6320194565507684 1105 96-97 0.6437384399665937 1105 98-99 0.6109126007071275 1105 100 0.4875753969440879 1106 1 0.47505049785316444 1106 2 3.78774470144673 1106 3 2.2336213700007086 1106 4 0.9982291133646015 1106 5 0.33326626340665655 1106 6 0.2529654978170157 1106 7 0.16411586194501382 1106 8 0.1286189031320646 1106 9 0.24865646138855624 1106 10-11 0.20600385379911046 1106 12-13 0.23422454754555133 1106 14-15 0.31421951528211167 1106 16-17 0.28337832525293294 1106 18-19 0.27461795817820445 1106 20-21 0.34207875526942644 1106 22-23 0.23699866271144288 1106 24-25 0.279516742754808 1106 26-27 0.23657808565257454 1106 28-29 0.17353425651266718 1106 30-31 0.16052559709728342 1106 32-33 0.2604072613616708 1106 34-35 0.3091260145674326 1106 36-37 0.18398141874403962 1106 38-39 0.08228180266930707 1106 40-41 0.31848640686290963 1106 42-43 0.23591422576351562 1106 44-45 0.31281387060783317 1106 46-47 0.2330074795539545 1106 48-49 0.13967975300725755 1106 50-51 0.0448369957126431 1106 52-53 0.06536991691030636 1106 54-55 0.08372168177893258 1106 56-57 0.05899135794639676 1106 58-59 0.10646021233208813 1106 60-61 0.06473975043745384 1106 62-63 0.0999674777605577 1106 64-65 0.11342405858275129 1106 66-67 0.02852160699272588 1106 68-69 -0.02034189209651771 1106 70-71 0.07625640569445835 1106 72-73 0.11100943562857424 1106 74-75 0.050261403328022425 1106 76-77 0.11895603034369628 1106 78-79 0.07327072702545934 1106 80-81 0.049641418304936735 1106 82-83 0.05939660809484337 1106 84-85 0.030844542823999177 1106 86-87 0.24979686393823286 1106 88-89 0.19069877923217504 1106 90-91 0.14800417105815455 1106 92-93 0.15603615678463 1106 94-95 0.2339553646004795 1106 96-97 0.04551133948053376 1106 98-99 0.10004202503123949 1106 100 0.019550088716936642 1107 1 0.42377693367737024 1107 2 3.530202893989248 1107 3 2.0727523390571996 1107 4 0.9091037133895412 1107 5 0.26457636559929654 1107 6 0.19555516910803306 1107 7 0.0926281665882911 1107 8 0.10488154101222591 1107 9 0.05080552148499606 1107 10-11 0.16885393412705696 1107 12-13 0.16311927253583036 1107 14-15 0.16981147837120858 1107 16-17 0.050089534482118836 1107 18-19 0.10906190961097906 1107 20-21 0.16339299189969836 1107 22-23 0.16219233962481638 1107 24-25 0.17406067735926456 1107 26-27 0.11447334212693505 1107 28-29 0.020557975956869257 1107 30-31 -0.0013349119409724608 1107 32-33 0.07103225152497572 1107 34-35 0.19732897489740964 1107 36-37 0.06579258693151502 1107 38-39 -0.1570065534710352 1107 40-41 -0.10660206283826312 1107 42-43 0.061685210607144825 1107 44-45 0.10548971132726592 1107 46-47 0.1179682356899292 1107 48-49 0.06972939652551702 1107 50-51 -0.05233245811785281 1107 52-53 -0.09196834667431375 1107 54-55 0.019277482158834403 1107 56-57 -0.06438322694516785 1107 58-59 -0.14248995528515707 1107 60-61 -0.05607302673028869 1107 62-63 0.008519377362127045 1107 64-65 0.04643457688263197 1107 66-67 -0.025150536340902363 1107 68-69 -0.08006929161394538 1107 70-71 -0.05643316402136378 1107 72-73 -0.1053548561477129 1107 74-75 -0.11889614598559461 1107 76-77 -0.08534157531449438 1107 78-79 -0.044402389686744925 1107 80-81 0.0242039730120851 1107 82-83 -0.011192921547802825 1107 84-85 0.04276516162158828 1107 86-87 0.06474194795266541 1107 88-89 0.10493005228787311 1107 90-91 0.016541766595146612 1107 92-93 0.0486537945497858 1107 94-95 0.0071356369769866035 1107 96-97 -0.07528748471688473 1107 98-99 0.05286330957619256 1107 100 -0.03855873597685644 1108 1 0.4182981619240529 1108 2 3.565905259053096 1108 3 2.1240190773679366 1108 4 0.9242280088722978 1108 5 0.280497491093719 1108 6 0.12577004895025823 1108 7 0.1274683506281704 1108 8 0.0012545955399687614 1108 9 0.028217164297551278 1108 10-11 0.06120623488741472 1108 12-13 0.023283283340532535 1108 14-15 0.03986485907788051 1108 16-17 -0.0055839928108056824 1108 18-19 -0.043947367613967 1108 20-21 0.04033590487404837 1108 22-23 -0.0540751105239039 1108 24-25 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0.2908176564136298 2210 74-75 -0.22658670918477242 2210 76-77 0.19315012533392917 2210 78-79 0.3328706583777148 2210 80-81 0.36248745400610716 2210 82-83 0.2639134561204628 2210 84-85 0.3601260659350487 2210 86-87 0.3554129448904355 2210 88-89 0.3765658154959972 2210 90-91 0.35696682972460536 2210 92-93 0.42472148723881276 2210 94-95 0.4160482954031117 2210 96-97 0.3148761957438815 2210 98-99 0.32631347473698824 2210 100 0.30757431528926205 2211 1 0.5225607676069366 2211 2 3.936963633769931 2211 3 2.22573040219212 2211 4 1.122648130856092 2211 5 0.4634498734407799 2211 6 0.23903932886389612 2211 7 0.1838421801524106 2211 8 0.25830828536304296 2211 9 0.182222888720446 2211 10-11 0.22589353359466458 2211 12-13 0.18794098434349138 2211 14-15 0.3085242006393045 2211 16-17 0.18196082914818135 2211 18-19 0.36784776099578664 2211 20-21 0.31859155437452813 2211 22-23 0.17901243123515087 2211 24-25 0.14198511275228043 2211 26-27 0.2787242740832241 2211 28-29 0.270056988421878 2211 30-31 0.26198205874296576 2211 32-33 0.43983382285459527 2211 34-35 0.42670309213088586 2211 36-37 0.2153470534444537 2211 38-39 0.2417158426092456 2211 40-41 0.16554309254734534 2211 42-43 0.26478176547507104 2211 44-45 0.3338763974523715 2211 46-47 0.1995242276566742 2211 48-49 0.23961891851536166 2211 50-51 0.1805973980174329 2211 52-53 0.25659260104346515 2211 54-55 0.19146302820356453 2211 56-57 0.1392566487746052 2211 58-59 0.3167848744777757 2211 60-61 0.37857477253977123 2211 62-63 0.4167617832325661 2211 64-65 0.27591933210983655 2211 66-67 0.27309687473004374 2211 68-69 0.2527700491373679 2211 70-71 0.27265767839399047 2211 72-73 0.24247733520760306 2211 74-75 0.20931800720055094 2211 76-77 0.3619169814175507 2211 78-79 0.19389875924466793 2211 80-81 0.1532983579209386 2211 82-83 -0.08600731982047449 2211 84-85 0.2020283977694035 2211 86-87 0.3042511287245482 2211 88-89 0.33859172940610804 2211 90-91 0.3782509230993938 2211 92-93 0.45229682838693464 2211 94-95 0.5045407864535925 2211 96-97 0.473102561063353 2211 98-99 0.49679529868733496 2211 100 0.37360892930592016 2212 1 0.4101476331477123 2212 2 4.054700626815176 2212 3 2.108194686638573 2212 4 1.1191357696953403 2212 5 0.388050428952873 2212 6 0.4041888894137813 2212 7 0.1629592957356607 2212 8 0.1867265648058165 2212 9 0.12229251556782827 2212 10-11 0.1896391147093226 2212 12-13 0.25075386486221163 2212 14-15 0.3233835803944416 2212 16-17 0.45186756942818107 2212 18-19 0.4518838710049664 2212 20-21 0.3738535994020964 2212 22-23 0.2852061256662779 2212 24-25 0.40004112372879774 2212 26-27 0.13583914045943146 2212 28-29 0.09800540210364517 2212 30-31 0.28631095857192435 2212 32-33 0.35025120826686873 2212 34-35 0.43145965148517007 2212 36-37 0.49366281578122795 2212 38-39 0.3746282624299724 2212 40-41 0.16564062941404245 2212 42-43 0.1841187767180017 2212 44-45 0.3382922670796944 2212 46-47 0.36741447076472866 2212 48-49 0.3786203271131541 2212 50-51 0.010497582992826437 2212 52-53 0.031167669448514346 2212 54-55 0.12195353524707286 2212 56-57 0.3181535654033283 2212 58-59 0.3145968433886708 2212 60-61 0.16429110155540627 2212 62-63 0.359678245835795 2212 64-65 0.3328998181880891 2212 66-67 0.31073852290319337 2212 68-69 0.3943768638969303 2212 70-71 0.47584661695506014 2212 72-73 0.44001637321837705 2212 74-75 0.4283084351461355 2212 76-77 0.4954875165985442 2212 78-79 0.39300665880030294 2212 80-81 0.4693165997275841 2212 82-83 0.3780434517374971 2212 84-85 0.4727047487325464 2212 86-87 0.47671629892983347 2212 88-89 0.5426111099470496 2212 90-91 0.4816370722439487 2212 92-93 0.38808603433000144 2212 94-95 0.4883887847248083 2212 96-97 0.3230636324799825 2212 98-99 0.49483336012314894 2212 100 0.5909317525536082 2213 1 -0.6372983076604868 2213 2 -25.873812776921625 2213 3 -14.800331054082063 2213 4 -6.305261619062158 2213 5 -2.0446124302949045 2213 6 -1.3114019610509615 2213 7 -1.1491483889499605 2213 8 -1.0446911748303194 2213 9 -1.046705642116109 2213 10-11 -1.1080428390820387 2213 12-13 -1.1401934745319195 2213 14-15 -1.4081602043226198 2213 16-17 -1.319185688991503 2213 18-19 -1.3074397350779137 2213 20-21 -1.317482576204938 2213 22-23 -1.3249968892291548 2213 24-25 -1.382558893042912 2213 26-27 -1.378320095120877 2213 28-29 -1.4974251293075582 2213 30-31 -1.3888484884554444 2213 32-33 -1.3837931565147983 2213 34-35 -1.3539017814982017 2213 36-37 -1.5778405007478042 2213 38-39 -1.49710157511592 2213 40-41 -1.385591294049334 2213 42-43 -1.4214313740473514 2213 44-45 -1.2134610714429215 2213 46-47 -1.2280309802683007 2213 48-49 -1.3109362977391328 2213 50-51 -1.463370699383134 2213 52-53 -1.483097804128569 2213 54-55 -1.3955119399763234 2213 56-57 -1.2723817866405795 2213 58-59 -1.224120861417866 2213 60-61 -1.437730558648667 2213 62-63 -1.3294346488846642 2213 64-65 -1.2981116961586707 2213 66-67 -1.3229685555313182 2213 68-69 -1.439071024693618 2213 70-71 -1.4681361194990785 2213 72-73 -1.3403320668322678 2213 74-75 -1.3958820853377318 2213 76-77 -1.4808214101198693 2213 78-79 -1.2939382605282717 2213 80-81 -1.3941289891833293 2213 82-83 -1.506032372867427 2213 84-85 -1.517656918334545 2213 86-87 -1.3484524857229232 2213 88-89 -1.34129648306007 2213 90-91 -1.1256548157518793 2213 92-93 -1.2098602540024999 2213 94-95 -1.4214393360870865 2213 96-97 -1.3719412266865056 2213 98-99 -1.3537401954130779 2213 100 -1.4868992842726492 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 9.0 4 17.0 5 32.0 6 29.0 7 29.0 8 22.0 9 56.0 10 256.0 11 1124.0 12 3344.0 13 6317.0 14 8582.0 15 9881.0 16 10698.0 17 11105.0 18 11434.0 19 11922.0 20 13579.0 21 15929.0 22 18165.0 23 19259.0 24 19039.0 25 18940.0 26 19885.0 27 22665.0 28 26357.0 29 31347.0 30 38179.0 31 46987.0 32 59280.0 33 80196.0 34 111220.0 35 158422.0 36 244625.0 37 418753.0 38 432711.0 39 2796.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.90847096659693 21.181665151849245 10.203182730005754 29.70668115154807 2 12.255502917720838 22.82036914404089 37.13514339564974 27.788984542588537 3 15.5019098385379 28.616348004345515 28.49681960500642 27.384922552110165 4 10.177530461745771 19.00093268492067 37.63108707710854 33.19044977622502 5 10.617840723031497 39.692191863008155 35.56482290498074 14.12514450897961 6 29.2232977037422 39.5718146093253 18.30938293379797 12.895504753134526 7 25.445743368455908 33.64648142313176 23.761675629776395 17.146099578635933 8 24.778835739204556 37.32319143072608 20.789226881097907 17.10874594897146 9 25.564473963192828 16.759710896188704 22.221664413773286 35.45415072684518 10-11 22.32073068832761 28.627118069250677 28.96740844431781 20.084742798103903 12-13 23.94297181569717 25.89436327295207 29.684537153370727 20.478127757980033 14-15 21.92574160655517 26.91606394944546 26.851252487510376 24.306941956489 16-17 19.892803157642053 30.752404559270275 28.707958429648766 20.646833853438906 18-19 20.48128079527984 29.527957454920813 30.65902900116359 19.331732748635755 20-21 21.188957358890644 28.717103673538258 30.12577435905377 19.968164608517327 22-23 20.16643456567568 28.776989749822185 29.600165104968738 21.45641057953339 24-25 20.265498231675945 29.041095012677413 29.418627592583054 21.274779163063588 26-27 20.683722260056108 28.319710019495947 29.8152890608336 21.181278659614346 28-29 20.221596368470106 28.323818712908533 30.528248985019644 20.926335933601717 30-31 20.934152353963437 28.978528341995847 29.657992940232266 20.42932636380845 32-33 19.523826683483147 28.928323089961495 30.289924125643132 21.25792610091223 34-35 19.443489411772244 28.950952369258104 30.600608183787674 21.00495003518198 36-37 20.970231326510454 28.819022908425023 29.79403238464372 20.4167133804208 38-39 20.88355504160417 28.703152766087964 29.7059185470694 20.707373645238473 40-41 20.270648319486103 28.967522628770702 29.750013080214284 21.01181597152891 42-43 20.77801156078005 28.64608498299838 29.76970268390124 20.80620077232033 44-45 21.380484727824776 28.206401337298033 29.39872041592489 21.014393518952307 46-47 20.74088193772572 28.848065755554558 30.138914695900848 20.27213761081887 48-49 21.18065549312035 28.901124955078156 29.6076727842251 20.310546767576387 50-51 20.559894060386647 29.094413874671943 29.403315418645693 20.942376646295717 52-53 20.02383242860351 29.295677775739676 28.730812371823266 21.94967742383355 54-55 20.58489556008549 29.44595516432583 28.433661597856602 21.53548767773208 56-57 21.12317153651801 28.590558731880883 28.751086559552007 21.535183172049095 58-59 20.528257883049434 28.327293715504904 29.6222030838625 21.522245317583163 60-61 21.11096428528525 28.96603918647814 28.871269789785458 21.051726738451148 62-63 19.92665212915462 29.19623825857407 29.183850760549664 21.693258851721648 64-65 19.72992981934361 29.006607452599958 29.62802676940714 21.63543595864929 66-67 20.94471531084255 28.772926270172366 29.068500216521066 21.213858202464014 68-69 21.199683872139097 28.63531020046351 28.842608909252082 21.32239701814531 70-71 20.460954600334603 28.87707930723845 28.954214790682226 21.70775130174472 72-73 20.869782988111215 28.728330783136823 29.12574493460488 21.27614129414708 74-75 21.427278431163145 28.234204167723504 28.739537305263717 21.598980095849633 76-77 20.58629973876677 28.696116475046686 29.711895923186482 21.00568786300006 78-79 21.086991052753117 28.779541064833168 29.34766387824376 20.785804004169954 80-81 20.45503690173828 28.859012027569385 29.254885482611094 21.431065588081243 82-83 19.886969272523856 29.02414029029426 28.748117022120034 22.34077341506185 84-85 20.186263525488503 28.90010445856268 28.593189024082598 22.320442991866223 86-87 20.79563145879293 28.455767879604576 28.771326429717014 21.97727423188548 88-89 20.42351632211842 28.15267451889465 29.42730237982677 21.996506779160157 90-91 20.771930405284017 28.489098897543546 29.00729267243784 21.731678024734595 92-93 19.822855680045784 28.802435313974573 29.124760991839487 22.249948014140152 94-95 19.566325727101358 28.691108113201402 29.549365055892274 22.193201103804967 96-97 20.68556935211003 28.41899813845277 29.068751257892355 21.826681251544837 98-99 20.734893494122037 28.338048124502517 28.825373708815423 22.101684672560022 100 20.019410118379277 28.520261715946383 29.359140097552945 22.10118806812139 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4.0 1 8.5 2 10.0 3 10.0 4 15.0 5 29.5 6 70.5 7 135.5 8 206.5 9 256.5 10 243.5 11 255.5 12 333.0 13 401.5 14 468.5 15 587.5 16 749.0 17 934.5 18 1140.0 19 1399.0 20 1741.5 21 2245.5 22 2954.0 23 3970.5 24 5462.5 25 7683.0 26 10748.0 27 14730.5 28 19891.5 29 26240.5 30 33338.0 31 40661.5 32 48018.0 33 55345.0 34 62409.5 35 68634.5 36 73971.0 37 77994.5 38 80849.0 39 82139.0 40 81706.5 41 80500.5 42 78035.5 43 74194.0 44 69705.5 45 65470.0 46 61910.5 47 59275.5 48 57072.0 49 59992.5 50 84453.0 51 81248.5 52 47634.0 53 34992.5 54 30509.0 55 27397.0 56 24736.5 57 21952.0 58 19372.0 59 16793.0 60 14230.0 61 11933.5 62 9714.0 63 7773.5 64 6223.5 65 4856.5 66 3748.5 67 2833.5 68 2227.0 69 1792.5 70 1363.0 71 1023.5 72 750.5 73 555.0 74 380.0 75 254.0 76 158.0 77 70.5 78 31.5 79 18.5 80 11.0 81 6.0 82 3.0 83 1.5 84 1.5 85 1.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.735908434519631 2 5.6804016666711155 3 0.0 4 0.005765550303091777 5 0.163837721112858 6 0.056694577980402464 7 0.03763623114518243 8 0.0443627064987895 9 0.04206716332255851 10-11 0.0439623210610748 12-13 0.0420404709600442 14-15 0.04489655374907577 16-17 0.035153841431351245 18-19 0.029148059865630647 20-21 0.02330243247499593 22-23 0.02386297208779652 24-25 0.020099348973278277 26-27 0.012892411094413556 28-29 0.02004596424824965 30-31 0.025117513125969264 32-33 0.0017350035634303958 34-35 0.004110623827204321 36-37 0.004537701627433343 38-39 0.006699782991092759 40-41 0.007313707328921976 42-43 0.007367092053950604 44-45 0.008781787267209233 46-47 0.01702972728413219 48-49 0.026585593064256525 50-51 0.022955431762309852 52-53 0.028640904977858685 54-55 0.024957358950883385 56-57 0.01660264948390317 58-59 0.015161261908130228 60-61 0.01214502494401277 62-63 0.01809742178470474 64-65 0.020579811498535924 66-67 0.02124712056139377 68-69 0.02856082789031574 70-71 0.02754651811477182 72-73 0.024743820050768875 74-75 0.025090820763454952 76-77 0.018551191947448076 78-79 0.01454733757030101 80-81 0.013239411807099635 82-83 0.008781787267209233 84-85 0.010863791543325708 86-87 0.008915249079780802 88-89 0.007393784416464918 90-91 0.008034401116808447 92-93 0.004777932890062167 94-95 0.002028619551087847 96-97 0.0011744639506298064 98-99 6.406167003435307E-4 100 0.10708975840742689 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1873195.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.14737967540529 #Duplication Level Percentage of deduplicated Percentage of total 1 93.2253529048783 80.311198720702 2 5.608153622961416 9.662554788705137 3 0.7554592177358415 1.952424961787226 4 0.18082325003012237 0.6230979669794278 5 0.07014167853091473 0.30212609057364676 6 0.03819512615415484 0.19742460207311874 7 0.02740468641599339 0.1652589347894803 8 0.015217289176611134 0.1048743670662364 9 0.01314297598763933 0.10190096482247102 >10 0.055412978517027645 0.8446377655491416 >50 0.0043939764275095945 0.2635340057109366 >100 0.004118985477602779 0.7198009821652274 >500 5.614219817729403E-4 0.35279604351427923 >1k 0.0014347450645308367 2.5083180696226064 >5k 6.238022019699289E-5 0.2771853758486889 >10k+ 1.2476044039398578E-4 1.6128663600904807 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 16919 0.9032161627593497 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 13094 0.6990195895248493 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 5158 0.27535841169766095 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 4533 0.2419929585547687 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 4228 0.22571061742103732 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 4042 0.2157810585657126 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 3829 0.20441011213461494 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 3062 0.16346402803765758 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 2864 0.15289385248198933 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 2637 0.1407755199004909 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 5.338472502862756E-5 0.0 0.0 0.0 6 0.0 5.338472502862756E-5 0.0 0.0 0.0 7 0.0 5.338472502862756E-5 0.0 1.0676945005725512E-4 0.0 8 0.0 5.338472502862756E-5 0.0 1.0676945005725512E-4 0.0 9 0.0 5.338472502862756E-5 0.0 2.1353890011451023E-4 0.0 10-11 0.0 5.338472502862756E-5 0.0 2.1353890011451023E-4 0.0 12-13 0.0 5.338472502862756E-5 0.0 2.1353890011451023E-4 0.0 14-15 0.0 5.338472502862756E-5 0.0 2.669236251431378E-4 0.0 16-17 0.0 8.007708754294133E-5 0.0 3.736930752003929E-4 0.0 18-19 0.0 1.334618125715689E-4 0.0 5.872319753149032E-4 0.0 20-21 0.0 1.6015417508588267E-4 0.0 7.473861504007858E-4 0.0 22-23 0.0 1.6015417508588267E-4 0.0 8.007708754294133E-4 0.0 24-25 0.0 1.6015417508588267E-4 0.0 9.075403254866685E-4 0.0 26-27 5.338472502862756E-5 1.6015417508588267E-4 0.0 9.342326880009823E-4 0.0 28-29 1.0676945005725512E-4 1.8684653760019646E-4 0.0 0.0012545410381727476 0.0 30-31 1.0676945005725512E-4 2.1353890011451023E-4 0.0 0.0032831605892605946 0.0 32-33 1.0676945005725512E-4 2.1353890011451023E-4 0.0 0.008434786554523154 0.0 34-35 1.0676945005725512E-4 2.1353890011451023E-4 0.0 0.016789496021503366 0.0 36-37 1.334618125715689E-4 2.40231262628824E-4 0.0 0.030616139803917905 0.0 38-39 1.6015417508588267E-4 2.936159876574516E-4 0.0 0.05800250374360384 0.0 40-41 1.6015417508588267E-4 3.2030835017176533E-4 0.0 0.09326311462501236 0.0 42-43 1.6015417508588267E-4 3.2030835017176533E-4 0.0 0.12486687184195985 0.0 44-45 1.6015417508588267E-4 3.2030835017176533E-4 0.0 0.1648253385258876 0.0 46-47 1.6015417508588267E-4 3.2030835017176533E-4 0.0 0.21498028769028318 0.0 48-49 1.8684653760019646E-4 3.2030835017176533E-4 0.0 0.26486831322953563 0.0 50-51 2.40231262628824E-4 3.2030835017176533E-4 0.0 0.3206553508844514 0.0 52-53 2.669236251431378E-4 3.2030835017176533E-4 0.0 0.38303540208040276 0.0 54-55 2.669236251431378E-4 3.2030835017176533E-4 0.0 0.44755084227749914 0.0 56-57 2.669236251431378E-4 3.2030835017176533E-4 0.0 0.5250921553815806 0.0 58-59 2.669236251431378E-4 3.2030835017176533E-4 0.0 0.6154991872175615 0.0 60-61 2.669236251431378E-4 3.2030835017176533E-4 0.0 0.7142075437954938 0.0 62-63 2.669236251431378E-4 3.2030835017176533E-4 0.0 0.8123286683981112 0.0 64-65 2.669236251431378E-4 3.2030835017176533E-4 0.0 0.921794047069312 0.0 66-67 2.669236251431378E-4 3.2030835017176533E-4 0.0 1.0479688446744733 0.0 68-69 3.2030835017176533E-4 3.2030835017176533E-4 0.0 1.1962716108040006 0.0 70-71 3.2030835017176533E-4 3.2030835017176533E-4 0.0 1.3841057658172269 0.0 72-73 3.2030835017176533E-4 3.2030835017176533E-4 0.0 1.5668950643152475 0.0 74-75 3.2030835017176533E-4 3.2030835017176533E-4 0.0 1.7402619588457156 0.0 76-77 3.2030835017176533E-4 3.2030835017176533E-4 0.0 1.9386129046895813 0.0 78-79 3.2030835017176533E-4 3.2030835017176533E-4 0.0 2.1576504314820397 0.0 80-81 3.2030835017176533E-4 3.2030835017176533E-4 0.0 2.400871238712467 0.0 82-83 3.2030835017176533E-4 3.2030835017176533E-4 0.0 2.6838369737267076 0.0 84-85 3.2030835017176533E-4 3.2030835017176533E-4 0.0 2.9618646216758 0.0 86-87 3.2030835017176533E-4 3.2030835017176533E-4 0.0 3.2442431247147256 0.0 88 3.2030835017176533E-4 3.2030835017176533E-4 0.0 3.49338963642333 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGTACT 140 5.205921E-4 20.146461 4 CGTCTAC 135 0.007091175 18.475122 1 CATGGGT 2720 0.0 18.319454 4 GAGTACT 4065 0.0 17.555498 12-13 AGTACTT 4410 0.0 17.087029 12-13 CTAGTAC 595 0.0 16.589872 3 CATGGGG 2995 0.0 15.695629 4 ATGGGTG 1295 0.0 15.245972 5 GTACTTT 4750 0.0 15.023209 14-15 AAGACCG 190 0.0040240837 14.844761 5 CCTATAC 565 2.1827873E-11 14.142989 3 AGACCGT 200 0.00570002 14.086327 6 CCTAGAC 715 0.0 13.805558 3 CATGGGC 2085 0.0 13.753059 4 TATACTG 995 0.0 13.700944 5 CTAGACT 800 0.0 13.514917 4 GTATAAG 780 0.0 13.429993 1 CTTACAC 885 0.0 13.278146 3 GTAATAC 535 2.1263986E-9 13.178871 3 GTACATG 25650 0.0 13.165955 1 >>END_MODULE