FastQCFastQC Report
Wed 25 May 2016
SRR1295127_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295127_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3407142
Sequences flagged as poor quality0
Sequence length100
%GC45

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG93100.020.6204431
GTATCAA59200.020.3952431
GTATAGA11650.018.655081
TACATGG99550.018.4925692
CATGGGG58400.018.1150744
TCAACGC62700.018.0092374
ATCAACG63900.017.8412443
ACATGGG101800.017.8214443
TATCAAC66650.017.7498472
CAACGCA62250.017.6339845
AACGCAG62950.016.7722646
GTATATA15750.016.4819451
GTATTAG13100.016.1293661
CGCAGAG67700.015.7526778
ACGCAGA67000.015.6413857
GTATAGG9000.014.757091
TATAACG2907.5331755E-414.5693032
GTATAAA18300.014.5151721
TACGACT2657.8955025E-414.4793094
GTACATA13400.014.4166971
Produced by FastQC (version 0.11.5)