##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295127_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3407142
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.793210262442834	33.0	27.0	33.0	15.0	33.0
2	23.014320506747296	33.0	6.0	33.0	2.0	33.0
3	25.314930225978255	33.0	15.0	33.0	15.0	33.0
4	26.06596701869191	33.0	15.0	37.0	2.0	37.0
5	27.639901712344248	33.0	15.0	37.0	2.0	37.0
6	28.34294285357053	33.0	22.0	37.0	6.0	37.0
7	28.174424194823697	33.0	15.0	37.0	6.0	37.0
8	28.794077264757384	33.0	27.0	37.0	6.0	37.0
9	29.873185209187056	37.0	27.0	37.0	15.0	37.0
10-11	29.38845416481027	37.0	27.0	37.0	10.5	37.0
12-13	28.245301193786464	33.0	22.0	37.0	4.0	37.0
14-15	30.034282838813294	37.0	27.0	37.0	10.5	40.0
16-17	30.453470680118407	37.0	27.0	40.0	10.5	40.0
18-19	30.5395887520978	37.0	27.0	40.0	10.5	40.0
20-21	30.053163619244515	37.0	27.0	40.0	6.0	40.0
22-23	30.778229671672037	37.0	27.0	40.0	15.0	40.0
24-25	30.88777808497562	37.0	27.0	40.0	15.0	40.0
26-27	29.46191089188534	35.0	24.5	40.0	4.0	40.0
28-29	30.148951672692245	37.0	27.0	40.0	10.5	40.0
30-31	30.816601568117797	37.0	27.0	40.0	15.0	40.0
32-33	29.58419696038498	33.0	27.0	40.0	6.0	40.0
34-35	29.281614473362133	33.0	22.0	37.0	6.0	40.0
36-37	29.57086173690442	33.0	27.0	37.0	6.0	40.0
38-39	29.696193026295937	33.0	24.5	37.0	6.0	40.0
40-41	28.835330168217233	33.0	22.0	37.0	6.0	40.0
42-43	29.514404301317644	33.0	22.0	37.0	6.0	40.0
44-45	29.34597838892538	33.0	22.0	37.0	6.0	40.0
46-47	28.95951665648218	33.0	22.0	37.0	6.0	40.0
48-49	28.86543839382098	33.0	22.0	37.0	6.0	40.0
50-51	28.779723885884415	33.0	22.0	37.0	6.0	40.0
52-53	28.977388673556902	33.0	22.0	37.0	6.0	40.0
54-55	28.957161309977685	33.0	22.0	37.0	6.0	40.0
56-57	28.924574614148753	33.0	22.0	37.0	6.0	40.0
58-59	28.782160825700835	33.0	22.0	37.0	10.5	40.0
60-61	28.737002009308682	33.0	22.0	37.0	10.5	40.0
62-63	28.618001245618764	33.0	22.0	37.0	10.5	40.0
64-65	28.18801990055008	33.0	22.0	37.0	6.0	40.0
66-67	28.414379412422495	33.0	22.0	37.0	10.5	40.0
68-69	27.99651335342055	33.0	22.0	37.0	6.0	37.0
70-71	28.123986614000824	33.0	22.0	37.0	10.5	37.0
72-73	28.134113870217327	33.0	22.0	37.0	10.5	37.0
74-75	28.20204221015737	33.0	22.0	37.0	15.0	37.0
76-77	27.989354420801952	33.0	22.0	37.0	15.0	37.0
78-79	27.511400757585097	33.0	22.0	37.0	10.5	37.0
80-81	27.347190695310026	33.0	22.0	33.0	10.5	37.0
82-83	27.446865584117127	33.0	22.0	33.0	15.0	37.0
84-85	27.371192043067182	30.0	22.0	33.0	15.0	37.0
86-87	27.16108530257911	27.0	22.0	33.0	15.0	37.0
88-89	27.086256751259562	27.0	22.0	33.0	15.0	37.0
90-91	27.251184570528615	27.0	22.0	33.0	15.0	37.0
92-93	27.211874057494523	27.0	22.0	33.0	15.0	37.0
94-95	27.090693460971103	27.0	22.0	33.0	15.0	37.0
96-97	27.169602118138897	27.0	22.0	33.0	15.0	37.0
98-99	27.22062141818568	27.0	22.0	33.0	15.0	37.0
100	26.917812348296607	27.0	22.0	33.0	15.0	37.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	7.354380231634845
1101	2	11.380971511490394
1101	3	8.532982463727102
1101	4	13.019551264975643
1101	5	10.89814159906281
1101	6	10.056285007344751
1101	7	9.617823172693853
1101	8	9.103824361840253
1101	9	7.367558267491901
1101	10-11	7.8765205806895295
1101	12-13	9.213612887169319
1101	14-15	8.135893584479557
1101	16-17	8.317378496775849
1101	18-19	8.319821360690604
1101	20-21	7.337524604283747
1101	22-23	7.5259604197961245
1101	24-25	7.485382066828553
1101	26-27	8.671405974844038
1101	28-29	8.36834752245019
1101	30-31	5.57636267720865
1101	32-33	7.066577490583896
1101	34-35	8.89749212501027
1101	36-37	8.444767498334532
1101	38-39	8.439236132950324
1101	40-41	9.212562633415683
1101	42-43	8.752013311605857
1101	44-45	8.766290557658138
1101	46-47	7.587058170698061
1101	48-49	5.5900860908496846
1101	50-51	6.958388036516663
1101	52-53	6.0778682930317665
1101	54-55	4.020917256437929
1101	56-57	6.284643756091519
1101	58-59	6.347276476841962
1101	60-61	6.817733706215261
1101	62-63	6.395358492035047
1101	64-65	6.849957136319219
1101	66-67	6.02076766151918
1101	68-69	6.8940483885360315
1101	70-71	6.39698988267352
1101	72-73	5.553704883906221
1101	74-75	4.9851446049693
1101	76-77	5.193633511351507
1101	78-79	5.278222469736612
1101	80-81	5.1755119656783535
1101	82-83	4.583584614246959
1101	84-85	4.397964631900788
1101	86-87	4.23823405932794
1101	88-89	4.211354808567847
1101	90-91	4.06480376859659
1101	92-93	3.929711550572634
1101	94-95	3.8658924830478156
1101	96-97	3.9457379836117106
1101	98-99	3.725683245362493
1101	100	3.929071187175797
1102	1	6.791170252777189
1102	2	10.613745500589413
1102	3	8.23040880148832
1102	4	12.85584872671392
1102	5	10.619752998763616
1102	6	9.720771908025977
1102	7	6.760076066798522
1102	8	8.058031793586053
1102	9	6.657519736365202
1102	10-11	7.605296782236657
1102	12-13	9.165723650908518
1102	14-15	6.32055814748966
1102	16-17	7.505819996052725
1102	18-19	7.560986894828439
1102	20-21	7.080545927229583
1102	22-23	6.6684749159613865
1102	24-25	7.07084330038224
1102	26-27	8.636892010523692
1102	28-29	7.763127471193133
1102	30-31	4.2927230543932
1102	32-33	4.479815991635235
1102	34-35	5.369628189898698
1102	36-37	5.400942220445135
1102	38-39	4.874048673940774
1102	40-41	6.49342187611774
1102	42-43	7.202493567072274
1102	44-45	6.594323030341009
1102	46-47	5.675909003016113
1102	48-49	3.8348358846886086
1102	50-51	5.143334254619472
1102	52-53	5.964322432165982
1102	54-55	5.511617262993891
1102	56-57	6.4867612976718405
1102	58-59	6.2205709790206285
1102	60-61	6.213683797525629
1102	62-63	4.461815002367043
1102	64-65	6.260102513685048
1102	66-67	3.8975283156449017
1102	68-69	6.274425345349837
1102	70-71	5.477981726573688
1102	72-73	5.127588174924448
1102	74-75	4.33679339541143
1102	76-77	3.456262102989804
1102	78-79	4.638284717027588
1102	80-81	4.649223972822753
1102	82-83	4.038860232079205
1102	84-85	3.1797608158966923
1102	86-87	3.486087819723597
1102	88-89	3.613606592429484
1102	90-91	3.4552869512195947
1102	92-93	3.3646622858407156
1102	94-95	3.217187377244418
1102	96-97	3.4828611948833412
1102	98-99	3.2589932993164545
1102	100	3.4453040203778578
1103	1	6.742555097519254
1103	2	11.065987331412835
1103	3	8.064776537708884
1103	4	12.209073107170969
1103	5	10.480846118932263
1103	6	9.155756355441216
1103	7	8.266944028541744
1103	8	8.136044250575665
1103	9	7.2078276341345955
1103	10-11	7.54126317732657
1103	12-13	8.844814580963941
1103	14-15	8.733566472158177
1103	16-17	8.211834151075298
1103	18-19	7.44651488575056
1103	20-21	8.594320876308945
1103	22-23	7.121385853843979
1103	24-25	5.324559150656899
1103	26-27	6.157673270167756
1103	28-29	7.176216348144582
1103	30-31	6.263923268995811
1103	32-33	8.162075871053524
1103	34-35	7.3346329920051225
1103	36-37	4.188005486501499
1103	38-39	6.465704352736825
1103	40-41	6.890333936865741
1103	42-43	7.657329671451613
1103	44-45	5.890319341943453
1103	46-47	6.641340017501786
1103	48-49	5.886067619897208
1103	50-51	6.806173513602911
1103	52-53	6.372267239142328
1103	54-55	5.213475942699496
1103	56-57	6.196986791498624
1103	58-59	5.720212287784541
1103	60-61	5.222605931505758
1103	62-63	2.842158753151285
1103	64-65	5.1350094643949475
1103	66-67	4.197098381294037
1103	68-69	5.379590238018011
1103	70-71	3.868854061760718
1103	72-73	4.616026120246904
1103	74-75	3.4930866050430467
1103	76-77	3.8496536194719475
1103	78-79	3.0520206574706172
1103	80-81	4.286462375023586
1103	82-83	3.644602623826632
1103	84-85	3.360212520814038
1103	86-87	2.9250677909380904
1103	88-89	3.179397586852126
1103	90-91	2.9344462332911228
1103	92-93	2.476702923327121
1103	94-95	2.9592381869894346
1103	96-97	3.1392047147188222
1103	98-99	2.942650130482395
1103	100	3.1341549604173196
1104	1	6.31920405773095
1104	2	9.820066953306991
1104	3	7.4386662604274
1104	4	12.320322994314065
1104	5	9.409125761368443
1104	6	8.190189550088252
1104	7	3.9768439330931002
1104	8	6.560972664093313
1104	9	6.665251934414869
1104	10-11	7.199790703920073
1104	12-13	7.290393875814658
1104	14-15	7.326644930418237
1104	16-17	7.254243136638724
1104	18-19	6.983195175315842
1104	20-21	7.760896480911345
1104	22-23	6.565827606288789
1104	24-25	6.08314224633499
1104	26-27	8.305058196157095
1104	28-29	7.32488351151969
1104	30-31	6.4856257282133924
1104	32-33	7.693257095640014
1104	34-35	8.151581262540308
1104	36-37	6.626220971684045
1104	38-39	7.442529859806012
1104	40-41	7.290200851997074
1104	42-43	7.341284739354897
1104	44-45	6.8073789025041975
1104	46-47	6.6915691584391475
1104	48-49	5.629702438551686
1104	50-51	5.801104616359151
1104	52-53	5.99279455079002
1104	54-55	4.939955734893694
1104	56-57	6.122440127686858
1104	58-59	3.861851865342153
1104	60-61	4.330674220219542
1104	62-63	3.9507864600949603
1104	64-65	2.8989166623325744
1104	66-67	3.32582686980113
1104	68-69	4.104777904904733
1104	70-71	2.491659150219821
1104	72-73	2.439101209365898
1104	74-75	3.141593128517421
1104	76-77	3.8430023959097994
1104	78-79	4.464964323167887
1104	80-81	3.185129918039671
1104	82-83	2.9852717424217836
1104	84-85	2.96989454250852
1104	86-87	0.7653708320969699
1104	88-89	0.29704521818236174
1104	90-91	2.283725074201673
1104	92-93	2.521069009874772
1104	94-95	2.533650906563423
1104	96-97	2.8848451076071946
1104	98-99	2.5893204858788046
1104	100	0.9590305141064199
1105	1	5.884929839695694
1105	2	-2.739829933809059
1105	3	0.43302615245841025
1105	4	8.812827380019947
1105	5	8.717380357317424
1105	6	8.24605140008357
1105	7	5.454325909612127
1105	8	6.142124291173076
1105	9	5.119771885461674
1105	10-11	5.2117249650679796
1105	12-13	5.177111563024866
1105	14-15	6.153856626417205
1105	16-17	6.310292774135593
1105	18-19	6.466669788277535
1105	20-21	7.296383843227023
1105	22-23	2.0963610336806973
1105	24-25	4.142109755212683
1105	26-27	7.213592465042797
1105	28-29	6.179530788558342
1105	30-31	5.4906914196862715
1105	32-33	7.288172210892306
1105	34-35	6.412974173573861
1105	36-37	6.43429935553765
1105	38-39	4.299867462853637
1105	40-41	5.681291270553679
1105	42-43	5.845883408377844
1105	44-45	6.460673084082828
1105	46-47	5.582563245631299
1105	48-49	5.305398568010471
1105	50-51	6.162769683267609
1105	52-53	5.772481052986965
1105	54-55	5.4983016218650995
1105	56-57	5.340702553894076
1105	58-59	1.9807119075232116
1105	60-61	4.631961985894666
1105	62-63	-0.621933465583286
1105	64-65	-0.15078272645469326
1105	66-67	0.977782032378407
1105	68-69	3.0661170911064026
1105	70-71	3.1663785482959206
1105	72-73	2.7963229019273506
1105	74-75	2.7836145156271925
1105	76-77	1.8942955408465032
1105	78-79	3.3193327128679435
1105	80-81	1.8522978496178268
1105	82-83	1.0272718523359963
1105	84-85	1.4592285878394051
1105	86-87	-0.17266151709625888
1105	88-89	1.8011257748057545
1105	90-91	1.4957586718007185
1105	92-93	1.8365928131680889
1105	94-95	1.0847022541266718
1105	96-97	2.135945570696439
1105	98-99	2.0460019962948124
1105	100	2.326055550250018
1106	1	6.117998724211773
1106	2	10.373371985084116
1106	3	6.752557330718158
1106	4	11.770359923798136
1106	5	9.757365364567292
1106	6	9.328804431880759
1106	7	6.633158295269837
1106	8	7.5008951907870625
1106	9	6.904906932757456
1106	10-11	5.701619824858138
1106	12-13	7.897337521189428
1106	14-15	7.750541169899687
1106	16-17	7.6744778371236215
1106	18-19	7.14862970229585
1106	20-21	8.253702927691084
1106	22-23	6.19785738372865
1106	24-25	4.875161651381067
1106	26-27	7.658928066310583
1106	28-29	6.234988980848993
1106	30-31	5.3028705513091765
1106	32-33	7.301821379822506
1106	34-35	6.717160701433581
1106	36-37	5.837484613563181
1106	38-39	7.013444586172266
1106	40-41	6.58883853216258
1106	42-43	6.0715982667007395
1106	44-45	4.244863225998074
1106	46-47	5.915695153418991
1106	48-49	5.807511258043402
1106	50-51	6.517937884207047
1106	52-53	5.6341298606140064
1106	54-55	5.133448791383675
1106	56-57	4.877990031669505
1106	58-59	4.919981243722852
1106	60-61	4.8343064495508585
1106	62-63	4.979057141950914
1106	64-65	5.2134847355658565
1106	66-67	2.87026546754484
1106	68-69	3.27192565036329
1106	70-71	2.743541406856501
1106	72-73	2.6927687092091084
1106	74-75	2.763926309061034
1106	76-77	3.4255849373239755
1106	78-79	4.112460918543984
1106	80-81	3.5743235572627157
1106	82-83	1.9445043407668159
1106	84-85	2.49105842879451
1106	86-87	3.344105529857405
1106	88-89	3.309490390106543
1106	90-91	2.9991365543099207
1106	92-93	2.7731211549795844
1106	94-95	1.2752579563760769
1106	96-97	1.2808715863995452
1106	98-99	1.7821195852859795
1106	100	2.670487618864719
1107	1	6.1749329100969845
1107	2	10.533294618321694
1107	3	6.280126048557658
1107	4	11.625394283944683
1107	5	9.571677335826301
1107	6	8.235039872422366
1107	7	3.8099469240760833
1107	8	6.496298608098311
1107	9	5.821046958591708
1107	10-11	4.807986951604274
1107	12-13	6.258622660352444
1107	14-15	6.327681167552651
1107	16-17	7.256118551997506
1107	18-19	6.450535980989763
1107	20-21	5.69837293736947
1107	22-23	5.896164436370601
1107	24-25	4.750662097164248
1107	26-27	7.440063505323959
1107	28-29	6.667783703894887
1107	30-31	5.215282251677266
1107	32-33	5.145938021422776
1107	34-35	6.848460534827456
1107	36-37	6.740480493556294
1107	38-39	6.724818426305259
1107	40-41	7.949678378856344
1107	42-43	6.389821294925078
1107	44-45	4.300895111027934
1107	46-47	3.7049693428177513
1107	48-49	4.271029733124198
1107	50-51	5.095613387765393
1107	52-53	4.03153947402415
1107	54-55	5.394421882314891
1107	56-57	5.457053332461619
1107	58-59	3.1526963405956963
1107	60-61	2.8676030064137663
1107	62-63	0.5034469731449711
1107	64-65	2.3795357221845066
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2103	4	12.359355773964143
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2103	10-11	7.738121161312733
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2104	4	12.406506980687173
2104	5	10.275323485956314
2104	6	9.226123551130332
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2206	100	1.6605515340072152
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2207	18-19	4.008406264703652
2207	20-21	7.515733146586502
2207	22-23	6.372880150877098
2207	24-25	4.238321786410342
2207	26-27	3.8624046655780973
2207	28-29	5.067602609162652
2207	30-31	5.359209188897751
2207	32-33	6.705830367665634
2207	34-35	6.327913427879697
2207	36-37	6.700710864101584
2207	38-39	6.8225645869961475
2207	40-41	3.9143054530527515
2207	42-43	5.469772525452562
2207	44-45	3.818956889852629
2207	46-47	5.422138678450974
2207	48-49	2.375721758092787
2207	50-51	5.379328048315653
2207	52-53	2.8264497197648915
2207	54-55	2.8658778345278613
2207	56-57	5.143878471717269
2207	58-59	3.701089357319809
2207	60-61	3.478483244638589
2207	62-63	4.251154582363345
2207	64-65	3.2908760343782433
2207	66-67	3.3337766471816686
2207	68-69	3.280324977973141
2207	70-71	3.4367157683435785
2207	72-73	3.630863995630744
2207	74-75	3.2294316940963483
2207	76-77	3.872489125894372
2207	78-79	4.002537889661447
2207	80-81	4.044292893164545
2207	82-83	3.6886928625045208
2207	84-85	2.999784651145294
2207	86-87	3.2078145860890466
2207	88-89	3.1111704839290724
2207	90-91	0.987902425302476
2207	92-93	-0.94543166343
2207	94-95	1.079615774144667
2207	96-97	-1.211290588720221
2207	98-99	0.5336080674476982
2207	100	1.8255205305569113
2208	1	0.9337953712073599
2208	2	8.633282029772698
2208	3	6.7036920983854245
2208	4	11.95305180293241
2208	5	10.1986864627968
2208	6	9.247818475812775
2208	7	7.829127226975103
2208	8	7.9644628625047105
2208	9	6.678393834696955
2208	10-11	6.803370094447246
2208	12-13	6.9871382033957445
2208	14-15	8.03926872254581
2208	16-17	7.323458842275269
2208	18-19	6.637160674927266
2208	20-21	6.673746792751562
2208	22-23	5.945136310227898
2208	24-25	6.272849074174072
2208	26-27	8.158092609940244
2208	28-29	7.24116748542961
2208	30-31	6.217421896287803
2208	32-33	8.483383784395109
2208	34-35	6.564363618803977
2208	36-37	7.10503577853979
2208	38-39	7.412604972470628
2208	40-41	7.758632502042108
2208	42-43	7.073928176883179
2208	44-45	6.090899482960154
2208	46-47	4.66864511193177
2208	48-49	4.766554599323182
2208	50-51	6.011442554888458
2208	52-53	5.661576119780751
2208	54-55	6.494070261253103
2208	56-57	6.558955252406086
2208	58-59	5.869758926665135
2208	60-61	5.292189661952374
2208	62-63	3.483812617359881
2208	64-65	3.371989779430251
2208	66-67	2.9920320455946197
2208	68-69	5.53554729288366
2208	70-71	5.480944916538167
2208	72-73	4.805463381089876
2208	74-75	2.133538757041414
2208	76-77	3.3886765192761175
2208	78-79	4.166612733223236
2208	80-81	3.709462702197513
2208	82-83	1.3814306133191359
2208	84-85	2.0220021029323227
2208	86-87	1.0771725506075036
2208	88-89	0.7661721734986884
2208	90-91	1.9668797197797865
2208	92-93	0.7963927482179471
2208	94-95	-1.1625537896626703
2208	96-97	1.4668412505633341
2208	98-99	0.20800478227924657
2208	100	1.7490653195025594
2209	1	5.337191242980332
2209	2	9.782177319566387
2209	3	5.6953011983480515
2209	4	-3.4899575294878495
2209	5	0.4957417060809739
2209	6	4.327272139988654
2209	7	2.2041956578064266
2209	8	5.33252230900473
2209	9	5.065743563932191
2209	10-11	5.302740130862954
2209	12-13	7.309624587419066
2209	14-15	6.793399821402499
2209	16-17	6.417668975569956
2209	18-19	5.393210832566332
2209	20-21	5.881303575484505
2209	22-23	5.455081194859023
2209	24-25	5.249638173002101
2209	26-27	6.977823378106006
2209	28-29	6.364839263954057
2209	30-31	4.260243920488662
2209	32-33	6.331843206494213
2209	34-35	5.578379828711512
2209	36-37	6.268676976890941
2209	38-39	6.9367459900635104
2209	40-41	7.267172550935175
2209	42-43	6.807746345186182
2209	44-45	6.538622600236131
2209	46-47	6.855648376052709
2209	48-49	5.209391212357293
2209	50-51	4.656586002486584
2209	52-53	4.9299532552708065
2209	54-55	5.3866547041440604
2209	56-57	5.111116667638871
2209	58-59	3.1820553648182752
2209	60-61	4.112459982447984
2209	62-63	4.23420265141516
2209	64-65	5.071832935418755
2209	66-67	4.597619396872204
2209	68-69	3.7776894642841228
2209	70-71	3.685709470790602
2209	72-73	0.7875483645209549
2209	74-75	2.386499842071551
2209	76-77	3.522673410825078
2209	78-79	3.52865783162261
2209	80-81	3.777447259460555
2209	82-83	3.148791493543449
2209	84-85	3.0809035494931187
2209	86-87	1.5140568779121146
2209	88-89	2.1255334277033953
2209	90-91	2.4028935918119316
2209	92-93	2.3492391704475324
2209	94-95	0.7148583710413483
2209	96-97	-0.008043758977631654
2209	98-99	0.3507845995704528
2209	100	-0.05805517471950594
2210	1	1.665312359362332
2210	2	-17.30317154709184
2210	3	-7.711004336661622
2210	4	-8.175824255866228
2210	5	-1.524118794554532
2210	6	2.4211971477989067
2210	7	-8.671596435314864
2210	8	-8.61094646983091
2210	9	-3.7078314834998594
2210	10-11	-1.1023901920693184
2210	12-13	3.4079097266081924
2210	14-15	-5.400219664128397
2210	16-17	-1.5187575869165464
2210	18-19	-0.34781313410633885
2210	20-21	2.4166033424732447
2210	22-23	2.5505728847347875
2210	24-25	2.017677825228649
2210	26-27	4.140378771128727
2210	28-29	3.491925954626506
2210	30-31	2.558641598728407
2210	32-33	4.6687468917069594
2210	34-35	2.5165944694070355
2210	36-37	3.942664512260894
2210	38-39	2.933432176367262
2210	40-41	4.103365602783406
2210	42-43	2.234374178023124
2210	44-45	3.330790136919479
2210	46-47	2.888935537860494
2210	48-49	2.889752674519368
2210	50-51	2.064161127231987
2210	52-53	2.377437386240036
2210	54-55	2.6678443790962802
2210	56-57	2.706728014671846
2210	58-59	1.706672784054156
2210	60-61	2.6252125840240907
2210	62-63	1.5262272567250754
2210	64-65	2.5287894007062235
2210	66-67	2.482047983685078
2210	68-69	3.125607093378658
2210	70-71	2.196942559724402
2210	72-73	0.9236128997437838
2210	74-75	0.29909896319367846
2210	76-77	-0.34168598364475855
2210	78-79	0.05256845987430836
2210	80-81	0.6055535885000651
2210	82-83	0.6712262078791618
2210	84-85	-1.271987762789216
2210	86-87	-0.9638721013410496
2210	88-89	-1.5799536181964626
2210	90-91	-1.8021958647533616
2210	92-93	-1.270420729472562
2210	94-95	-0.510322814656675
2210	96-97	-0.07425158160859624
2210	98-99	-0.4739343154303519
2210	100	-0.20658959253730202
2211	1	1.0347727003750293
2211	2	-17.30317154709184
2211	3	-8.162706203304875
2211	4	-19.465771129589154
2211	5	-21.505524597578642
2211	6	-13.67117135035081
2211	7	-11.58848739557824
2211	8	-19.223753111219555
2211	9	-16.14473895923613
2211	10-11	-10.197327520976394
2211	12-13	-22.768632978745302
2211	14-15	-18.193067254382814
2211	16-17	-16.114443371401997
2211	18-19	-19.42927300821514
2211	20-21	-8.643394519433006
2211	22-23	-4.429105992637336
2211	24-25	-2.178247750841283
2211	26-27	-17.694113056432684
2211	28-29	-3.9635809724731423
2211	30-31	-4.803186130628802
2211	32-33	-1.0163859023789001
2211	34-35	-5.893422303383527
2211	36-37	-6.97862800420474
2211	38-39	-2.6966002132486153
2211	40-41	-5.479604834068336
2211	42-43	-1.8985877781490004
2211	44-45	-0.19129763845830539
2211	46-47	0.5286313598956696
2211	48-49	0.39050650725129543
2211	50-51	-4.993787891852438
2211	52-53	-1.325996979634457
2211	54-55	-0.8318329671375047
2211	56-57	-6.974363394286755
2211	58-59	-2.4644823080807896
2211	60-61	-1.341108289928851
2211	62-63	-0.648116806656784
2211	64-65	-3.7420619976532485
2211	66-67	-2.820239310115813
2211	68-69	0.06428637803946913
2211	70-71	-0.3747688541900267
2211	72-73	-1.369254234867828
2211	74-75	-2.020644265202172
2211	76-77	-2.034442255591035
2211	78-79	-1.3813257965105379
2211	80-81	-1.2380459095144118
2211	82-83	-1.7035683185615547
2211	84-85	-1.913383354623285
2211	86-87	-2.272913566511779
2211	88-89	-2.8276420805534883
2211	90-91	-2.500802995379498
2211	92-93	-2.4314070595205806
2211	94-95	-2.636061092928447
2211	96-97	-3.3171983766630255
2211	98-99	-3.2772418197662034
2211	100	-2.6083541042470735
2212	1	-21.403343137164025
2212	2	-17.30317154709184
2212	3	-20.239610793547605
2212	4	-19.465771129589154
2212	5	-21.505524597578642
2212	6	-22.256367216729085
2212	7	-22.79276521341049
2212	8	-23.14588721359879
2212	9	-24.6369283367896
2212	10-11	-11.845239673460878
2212	12-13	-22.768632978745302
2212	14-15	-24.16278607197474
2212	16-17	-24.562893263254917
2212	18-19	-19.111369044883993
2212	20-21	-24.24349065017081
2212	22-23	-19.25008678388609
2212	24-25	-25.865342199291174
2212	26-27	-17.714896496173466
2212	28-29	-18.718341473564276
2212	30-31	-6.428764767440818
2212	32-33	-17.79517818588984
2212	34-35	-14.176452715360439
2212	36-37	-18.23202244369875
2212	38-39	-24.307270725144793
2212	40-41	-10.742479942070444
2212	42-43	-14.474297434971286
2212	44-45	-17.699426871158476
2212	46-47	-17.64320027214267
2212	48-49	-18.33016359873091
2212	50-51	-9.485921536773073
2212	52-53	-2.361358272064546
2212	54-55	-24.17982623798586
2212	56-57	-24.06268350964364
2212	58-59	-13.032065245003693
2212	60-61	-6.416333556076335
2212	62-63	-3.2807106033892417
2212	64-65	-17.88175258811633
2212	66-67	-18.202978108339842
2212	68-69	-15.037152361488111
2212	70-71	-10.02462726753029
2212	72-73	-18.08200463707703
2212	74-75	-6.580347445441504
2212	76-77	-11.647950041729782
2212	78-79	-4.941685268770826
2212	80-81	-2.7388936254107143
2212	82-83	-3.3432489222522825
2212	84-85	-5.669422549161101
2212	86-87	-5.495734501106153
2212	88-89	-3.537852878767211
2212	90-91	-4.348571467203588
2212	92-93	-5.6655133690344925
2212	94-95	-4.015762752601152
2212	96-97	-5.671880401052658
2212	98-99	-4.037258777949976
2212	100	-4.199169881801062
2213	1	-21.403343137164025
2213	2	-17.30317154709184
2213	3	-20.239610793547605
2213	4	-19.465771129589154
2213	5	-21.505524597578642
2213	6	-22.256367216729085
2213	7	-10.99725486616391
2213	8	-10.707444561897633
2213	9	-4.172529880107735
2213	10-11	-18.136764776829907
2213	12-13	-16.700586679201283
2213	14-15	-24.16278607197474
2213	16-17	-18.45065194441942
2213	18-19	-19.037350333609176
2213	20-21	-18.22086700934303
2213	22-23	-20.34327575948658
2213	24-25	-7.8167627534826885
2213	26-27	-23.81007075890805
2213	28-29	-24.74836828321575
2213	30-31	-20.017626208278564
2213	32-33	-23.956304191251768
2213	34-35	-18.472551539628864
2213	36-37	-24.373845499999018
2213	38-39	-11.917407519252123
2213	40-41	-17.69958393549545
2213	42-43	-23.928989123979328
2213	44-45	-17.74851928311496
2213	46-47	-17.56384069462742
2213	48-49	-6.433761884643477
2213	50-51	-24.050264700311946
2213	52-53	-18.44794131568877
2213	54-55	-18.29645198333837
2213	56-57	-18.109859938966686
2213	58-59	-18.558892951382056
2213	60-61	-19.985253355629286
2213	62-63	-18.07040285191123
2213	64-65	-18.095574651396888
2213	66-67	-8.954996919292775
2213	68-69	-23.333935203311167
2213	70-71	-17.97038854972938
2213	72-73	-9.746324103554624
2213	74-75	-4.5476487921304525
2213	76-77	-3.4685392146177136
2213	78-79	-4.95871508663258
2213	80-81	-3.5692812510310006
2213	82-83	-3.5310122111787017
2213	84-85	-3.2859976091325365
2213	86-87	-3.439829748246755
2213	88-89	-3.062727928488723
2213	90-91	-3.021002757480364
2213	92-93	-3.87292199194372
2213	94-95	-3.9162340608199884
2213	96-97	-3.5687438316912683
2213	98-99	-4.002141425776852
2213	100	-3.4774106194943393
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	843.0
3	783.0
4	3851.0
5	15131.0
6	24534.0
7	28422.0
8	25440.0
9	24873.0
10	28777.0
11	35484.0
12	44517.0
13	59321.0
14	44561.0
15	49510.0
16	57942.0
17	62336.0
18	62840.0
19	63702.0
20	62963.0
21	61766.0
22	64789.0
23	72470.0
24	80074.0
25	89478.0
26	98930.0
27	107726.0
28	117938.0
29	125959.0
30	135105.0
31	150183.0
32	172419.0
33	208813.0
34	253607.0
35	296095.0
36	321450.0
37	271937.0
38	82358.0
39	215.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.89266690340909	20.599222554788962	14.20552835836039	24.302582183441558
2	14.998200761085783	21.234841605199108	39.93248606941201	23.8344715643031
3	17.268932632648585	26.809113725700573	29.992261860390983	25.929691781259855
4	12.295248371197738	17.7296019068171	39.27817649336637	30.696973228618795
5	13.594585700371967	37.21054538564586	35.5125268836863	13.68234203029588
6	30.219480991132574	37.828762257057804	19.028921262283784	12.922835489525838
7	25.90626954814561	32.35749202786013	24.332289491553926	17.403948932440336
8	24.057974823768944	35.55306035577004	22.145941621300043	18.243023199160962
9	24.878884027917252	16.82669499694715	21.857537598946834	36.43688337618876
10-11	23.523926039327176	26.954953187397873	29.11087655288706	20.410244220387888
12-13	24.20384510468087	24.713618422354322	29.144249647344484	21.93828682562032
14-15	21.837981214571826	25.895453496422803	26.263463891321457	26.00310139768391
16-17	21.634959698946574	29.35999786577029	27.059904842573562	21.945137592709575
18-19	21.687512756574215	28.563514609559988	28.307376751444224	21.441595882421574
20-21	23.046089254760112	27.974434353801325	27.269556506939374	21.709919884499186
22-23	22.209687535778823	27.940039051805243	27.819822213483203	22.03045119893273
24-25	22.433592785831063	27.710709794024673	27.30519461414893	22.550502805995333
26-27	22.34789461763557	27.792743066324665	27.409294814166785	22.45006750187298
28-29	22.124953895261616	27.695548641300988	27.832802662643513	22.346694800793884
30-31	22.68765832851082	28.00798684699669	27.126766285081384	22.177588539411104
32-33	22.035305264165014	27.90181473886581	27.66172455896513	22.401155438004047
34-35	22.173620855611514	27.319847374114637	27.49552832904685	23.011003441227
36-37	22.592475489972017	27.896385336318787	27.16307833531351	22.348060838395686
38-39	22.259421373437345	27.599032219400595	27.479158474151085	22.66238793301098
40-41	22.61008036486272	27.632677208249678	27.049931600983008	22.70731082590459
42-43	22.252159600914737	27.823731915000877	27.588049422872224	22.33605906121216
44-45	22.63496736454963	27.307142753307506	27.410386353024958	22.6475035291179
46-47	22.5036365978422	27.753639362575544	27.44174628968892	22.300977749893345
48-49	22.298454978508524	27.424570075172483	27.602588893084206	22.674386053234784
50-51	22.62611164311529	27.532517579654826	27.244169223735877	22.597201553494013
52-53	22.126404191322234	27.584497031049555	27.550288607674194	22.738810169954014
54-55	22.481746177547244	27.518084291849092	27.253092546448304	22.747076984155363
56-57	22.585176352486716	27.534596099095214	27.250691598750976	22.629535949667094
58-59	22.37620658080522	27.049716253370644	27.59493370628962	22.979143459534516
60-61	22.694495998561006	27.52110846636029	27.146492649472354	22.637902885606355
62-63	22.22342408672816	27.638404418821498	27.568564981354687	22.569606513095653
64-65	22.37211124816917	27.13591478273228	27.41879463499812	23.07317933410043
66-67	22.42328741362643	27.41191112617135	27.23396700486113	22.93083445534109
68-69	22.687079694014617	27.242576531092876	27.271980383429938	22.798363391462566
70-71	22.85549217761312	27.28722094420673	27.0044556320494	22.85283124613075
72-73	22.48872374169266	27.519926518239828	27.428580965464377	22.562768774603136
74-75	22.667054231744686	27.093920183782767	27.08835818113638	23.15066740333617
76-77	22.709532948507118	27.427945954236794	27.15367049979841	22.708850597457676
78-79	22.550811096480622	27.162461147090173	27.426466855967302	22.860260900461906
80-81	22.830139509806123	27.144220040587392	27.091696058829257	22.933944390777224
82-83	22.31315396942664	27.343389366990507	27.545412258185227	22.79804440539763
84-85	22.545709385414835	26.99577047785221	27.09972567831623	23.35879445841673
86-87	22.68018076783433	27.331840643987988	27.187672149049586	22.800306439128097
88-89	22.471988013922655	26.935205258617952	27.469685557648997	23.1231211698104
90-91	22.70944665950251	26.988564882356048	27.089725826016426	23.21226263212501
92-93	22.321219333254042	27.23346295024901	27.43829555718591	23.007022159311045
94-95	22.40224641550395	26.772160181630106	27.351144505439944	23.474448897425997
96-97	22.492287525533648	27.018493413042183	27.40941420606221	23.079804855361964
98-99	22.349261329313823	26.862571143415366	27.425864952506178	23.36230257476463
100	22.676677447946403	26.530820380421087	27.210769057088513	23.581733114543997
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	341.0
1	431.0
2	423.5
3	322.0
4	356.5
5	483.0
6	657.5
7	936.5
8	1415.5
9	2042.0
10	2971.5
11	4032.0
12	5078.5
13	6122.0
14	7188.5
15	8236.0
16	9099.5
17	9949.0
18	10798.0
19	11533.5
20	12400.0
21	13398.0
22	14426.0
23	15595.5
24	17214.0
25	19388.5
26	22512.5
27	27184.0
28	33744.5
29	41516.0
30	50532.0
31	60033.5
32	69632.0
33	79952.5
34	89578.0
35	98477.5
36	107137.0
37	115039.5
38	121162.5
39	125843.5
40	128985.5
41	129792.5
42	128807.0
43	126799.5
44	123420.5
45	118517.5
46	113458.0
47	108826.0
48	103883.0
49	99942.0
50	98158.5
51	94258.5
52	87586.0
53	82258.0
54	77554.0
55	72630.5
56	68082.5
57	62765.0
58	57031.0
59	51574.5
60	45709.0
61	39780.0
62	34147.0
63	28597.0
64	23414.5
65	18972.5
66	15074.0
67	11674.5
68	9079.5
69	7139.5
70	5534.5
71	4278.5
72	3323.0
73	2555.5
74	1958.0
75	1437.0
76	1020.5
77	691.0
78	462.0
79	302.0
80	194.0
81	127.5
82	96.0
83	76.0
84	49.5
85	30.0
86	24.5
87	14.5
88	10.5
89	7.5
90	5.0
91	3.0
92	0.5
93	1.0
94	1.0
95	0.5
96	0.0
97	0.5
98	1.5
99	1.0
100	2.5
>>END_MODULE
>>Per base N content	fail
#Base	N-Count
1	7.432094112895793
2	23.98367898960478
3	5.89775829712997
4	21.930756041280347
5	11.705176948891475
6	9.405272806357939
7	7.8499223102529925
8	8.43445914493731
9	5.157841968429845
10-11	6.653523686421053
12-13	9.512518116356759
14-15	7.162953583971551
16-17	7.58597675118912
18-19	6.674479666535765
20-21	7.645630854246756
22-23	5.6631041500471655
24-25	5.2291040408647484
26-27	9.484591484593246
28-29	6.221387309363684
30-31	4.271307154207251
32-33	8.584291467746281
34-35	6.953232357207302
36-37	6.893431503588639
38-39	5.846058074480019
40-41	7.871934894407101
42-43	6.264443923969121
44-45	6.116460658229096
46-47	5.783953237053225
48-49	3.8061078757504094
50-51	6.396680854510907
52-53	4.37822961297181
54-55	4.780839777150468
56-57	4.657583980943559
58-59	3.487409682367216
60-61	4.055980056011754
62-63	3.1320825489515847
64-65	4.3848627383302485
66-67	2.297761584342537
68-69	4.973992865574725
70-71	2.3843737654609054
72-73	1.5189123318018445
74-75	0.7943461117851854
76-77	1.0696061391042697
78-79	1.7014847047760264
80-81	2.015384154813624
82-83	0.7802580579265554
84-85	1.2253671845787466
86-87	0.44839633921920485
88-89	0.7303628671772412
90-91	0.3248617169463439
92-93	0.30717827434254286
94-95	0.5364173257234363
96-97	0.5422726731084293
98-99	0.4700860721390538
100	0.42654518068222574
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3407142.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	90.77688115474119
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.6403520071556	85.91155986596436
2	3.8749007634136863	7.035028121736402
3	0.765661106582013	2.0851298183100906
4	0.2717697792543985	0.9868165181130711
5	0.14247900704593544	0.6466899944827207
6	0.08156788688533807	0.4442687024300225
7	0.04644726079962392	0.2951436231499511
8	0.03564884686124575	0.25888729078614997
9	0.026428807124631446	0.21592122150728213
>10	0.11035738140463087	1.6299275117811702
>50	0.003167578380474898	0.1958578875725191
>100	0.0011220642603815218	0.19837411607588226
>500	3.2503614552546205E-5	0.0151364587548737
>1k	6.50072174999362E-5	0.0812588693355355
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	2.9350112205478963E-5	0.0
4	0.0	0.0	0.0	2.9350112205478963E-5	0.0
5	0.0	0.0	0.0	2.9350112205478963E-5	0.0
6	0.0	0.0	0.0	2.9350112205478963E-5	0.0
7	0.0	0.0	0.0	5.8700224410957926E-5	0.0
8	0.0	0.0	0.0	8.805033661643689E-5	0.0
9	0.0	0.0	0.0	8.805033661643689E-5	0.0
10-11	0.0	0.0	0.0	8.805033661643689E-5	0.0
12-13	0.0	0.0	0.0	8.805033661643689E-5	0.0
14-15	0.0	1.4675056102739481E-5	0.0	8.805033661643689E-5	0.0
16-17	0.0	2.9350112205478963E-5	0.0	8.805033661643689E-5	0.0
18-19	0.0	2.9350112205478963E-5	0.0	1.1740044882191585E-4	0.0
20-21	0.0	2.9350112205478963E-5	0.0	1.3207550492465533E-4	0.0
22-23	0.0	5.8700224410957926E-5	0.0	1.614256171301343E-4	2.9350112205478963E-5
24-25	0.0	5.8700224410957926E-5	0.0	2.2012584154109223E-4	2.9350112205478963E-5
26-27	0.0	5.8700224410957926E-5	0.0	2.494759537465712E-4	2.9350112205478963E-5
28-29	0.0	8.805033661643689E-5	0.0	3.5220134646574754E-4	2.9350112205478963E-5
30-31	2.9350112205478963E-5	8.805033661643689E-5	0.0	0.0013354301053492927	4.402516830821844E-5
32-33	2.9350112205478963E-5	8.805033661643689E-5	0.0	0.004006290316047878	5.8700224410957926E-5
34-35	2.9350112205478963E-5	8.805033661643689E-5	0.0	0.008438157259075202	7.33752805136974E-5
36-37	2.9350112205478963E-5	8.805033661643689E-5	0.0	0.016641513620506573	8.805033661643689E-5
38-39	2.9350112205478963E-5	8.805033661643689E-5	0.0	0.03021594051554059	8.805033661643689E-5
40-41	2.9350112205478963E-5	8.805033661643689E-5	0.0	0.04941091389792383	8.805033661643689E-5
42-43	2.9350112205478963E-5	1.1740044882191585E-4	0.0	0.06831238615825228	8.805033661643689E-5
44-45	2.9350112205478963E-5	1.467505610273948E-4	0.0	0.09108807322970396	8.805033661643689E-5
46-47	2.9350112205478963E-5	1.467505610273948E-4	0.0	0.11867717870285419	8.805033661643689E-5
48-49	2.9350112205478963E-5	1.467505610273948E-4	0.0	0.14975894752845642	8.805033661643689E-5
50-51	2.9350112205478963E-5	1.467505610273948E-4	0.0	0.1835115765647572	8.805033661643689E-5
52-53	2.9350112205478963E-5	1.467505610273948E-4	0.0	0.21877573637964018	8.805033661643689E-5
54-55	2.9350112205478963E-5	1.467505610273948E-4	0.0	0.2590440903255573	8.805033661643689E-5
56-57	4.402516830821844E-5	1.467505610273948E-4	0.0	0.3094529080384674	1.0272539271917637E-4
58-59	5.8700224410957926E-5	1.467505610273948E-4	0.0	0.3664655009976103	1.1740044882191585E-4
60-61	5.8700224410957926E-5	1.467505610273948E-4	0.0	0.42567935237216414	1.1740044882191585E-4
62-63	5.8700224410957926E-5	1.467505610273948E-4	0.0	0.48954519653128636	1.1740044882191585E-4
64-65	5.8700224410957926E-5	1.467505610273948E-4	0.0	0.5614236213225043	1.1740044882191585E-4
66-67	5.8700224410957926E-5	1.467505610273948E-4	0.0	0.6481972280580028	1.1740044882191585E-4
68-69	5.8700224410957926E-5	1.467505610273948E-4	0.0	0.7477528086589875	1.1740044882191585E-4
70-71	5.8700224410957926E-5	1.467505610273948E-4	0.0	0.861910070082198	1.1740044882191585E-4
72-73	5.8700224410957926E-5	1.467505610273948E-4	0.0	0.9838891364081684	1.467505610273948E-4
74-75	5.8700224410957926E-5	1.467505610273948E-4	0.0	1.1150401127983511	1.467505610273948E-4
76-77	8.805033661643689E-5	2.0545078543835274E-4	0.0	1.2603525183276776	1.467505610273948E-4
78-79	1.467505610273948E-4	2.0545078543835274E-4	0.0	1.425931176334887	1.467505610273948E-4
80-81	1.467505610273948E-4	2.0545078543835274E-4	0.0	1.6110276589587402	1.467505610273948E-4
82-83	1.467505610273948E-4	2.0545078543835274E-4	0.0	1.8145266619354286	1.467505610273948E-4
84-85	1.467505610273948E-4	2.0545078543835274E-4	0.0	2.02741770081787	1.467505610273948E-4
86-87	1.467505610273948E-4	2.0545078543835274E-4	0.0	2.258872685670277	1.467505610273948E-4
88	1.467505610273948E-4	2.0545078543835274E-4	0.0	2.4534345794803976	1.467505610273948E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATG	9310	0.0	20.620443	1
GTATCAA	5920	0.0	20.395243	1
GTATAGA	1165	0.0	18.65508	1
TACATGG	9955	0.0	18.492569	2
CATGGGG	5840	0.0	18.115074	4
TCAACGC	6270	0.0	18.009237	4
ATCAACG	6390	0.0	17.841244	3
ACATGGG	10180	0.0	17.821444	3
TATCAAC	6665	0.0	17.749847	2
CAACGCA	6225	0.0	17.633984	5
AACGCAG	6295	0.0	16.772264	6
GTATATA	1575	0.0	16.481945	1
GTATTAG	1310	0.0	16.129366	1
CGCAGAG	6770	0.0	15.752677	8
ACGCAGA	6700	0.0	15.641385	7
GTATAGG	900	0.0	14.75709	1
TATAACG	290	7.5331755E-4	14.569303	2
GTATAAA	1830	0.0	14.515172	1
TACGACT	265	7.8955025E-4	14.479309	4
GTACATA	1340	0.0	14.416697	1
>>END_MODULE