FastQCFastQC Report
Wed 25 May 2016
SRR1295127_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295127_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3407142
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT39760.11669604612898435No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA89050.024.2429081
CAACGCA99850.021.282335
TCAACGC100650.021.2533024
ATCAACG102200.020.9288143
TATCAAC102700.020.8122752
AACGCAG102100.020.6993226
ACGCAGA112450.018.7913787
CGCAGAG114150.018.4703878
GTACATG124300.018.0466541
ACATGGG126950.017.0707383
TACATGG131650.017.0266932
GCAGAGT129250.016.0214149
TACGACG1800.00280594515.6713915
GAGTACT40500.015.18740712-13
CATGGGG73100.015.1783094
GGTATCA66050.015.1026321
TATACAG18700.014.8333475
AGTACTT47700.014.61757612-13
GTATATA17100.014.5111981
GTATTAG12700.014.4586391
Produced by FastQC (version 0.11.5)