FastQCFastQC Report
Wed 25 May 2016
SRR1295126_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295126_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3241674
Sequences flagged as poor quality0
Sequence length100
%GC42

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT38450.11861155686845747No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTCG1350.00710232618.4705379
GTATACG2502.4116605E-416.923011
GTATATA25950.016.3034781
GTCTCGC4654.2611646E-815.5972441
GTACATG147400.014.9253131
CTAATAC16250.014.4656053
TACATCG3351.3570845E-414.4300962
CTGTCGC6600.014.3566459
TACATGG148800.014.3349382
GTATAAG12650.014.3334361
TACGGGT3101.6438495E-414.1083344
GTACTAA11000.013.7362091
TCTGTCG5852.3101165E-1013.6360678
GGACCGT2200.007230813.5896836
GTATTAT17000.013.5099671
ACATGGG146900.013.4712613
TACACCG2952.463555E-413.4511665
GGTATAC5905.3933036E-813.317141
GTATTAA17750.013.2796261
TAACCGT2650.001622359713.16233356
Produced by FastQC (version 0.11.5)